CN106069696A - Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage - Google Patents
Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage Download PDFInfo
- Publication number
- CN106069696A CN106069696A CN201610530778.2A CN201610530778A CN106069696A CN 106069696 A CN106069696 A CN 106069696A CN 201610530778 A CN201610530778 A CN 201610530778A CN 106069696 A CN106069696 A CN 106069696A
- Authority
- CN
- China
- Prior art keywords
- cotton
- salt
- resistance
- semi
- wild
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to plant stress-resistance morphology and physiology field, the method being specifically related to semi-wild cotton secondary saline and alkaline Resistance Identification in seedling stage.Described method includes step: seed preparation；Germination；Transplant seedlings water planting；Stress treatment；Character is investigated；Evaluation of resistance.The present invention is first in the secondary saline and alkaline resistance that qualification semi-wild in seedling stage in indoor is cotton, and qualification is not subject to seasonal restrictions, and is not affected by outside climatic；Qualification cycle is short, land for growing field crops cotton in seedling stage Tolerant salt about 50d, and indoor water planting is identified and only needed about 25d.
Description
Technical field
The present invention relates to plant stress-resistance morphology and physiology field, be specifically related to semi-wild cotton secondary saline and alkaline resistance in seedling stage
The method identified.
Background technology
Semi-wild cotton (semi-wild cotton) is Gossypium hirsutum L. Races (race), originates in the Mexico in Central America
Highland and Caribbean.Semi-wild cotton genetic diversity enriches, and under environment stress, has the cultivation of many conventional land cottons
Plant the Phenotype not possessed.
In cotton in seedling stage anti-salt identification research, due to (1) field test cycle length, limited by growing environment, survey nature
Shape climate condition limited, easy affects, and causes qualification result instability, identifies and can only complete in particular season；(2) indoor qualification
No matter using sand culture or water planting authentication method, the most only considering single salt stress (NaCl), and often have ignored actual life
The salt that in product, alkaline land soil is complicated is grouped into the impact on cotton seedling of the higher alkali content with association, as there is the world in China
The Xinjiang region of the title in saline-alkali soil museum, saline-alkali soil area is big, salification heavy and ion composition is complicated, and sieve occurs in mono-salt evaluation meeting
The problem that the anti-salt material selected may not apply to practice；(3) current index selected by cotton in seedling stage Salt-Tolerance Identification is multiple
Various, have phenotypic correlation index, Physiology and biochemistry index of correlation etc., the mensuration of some index not only extend Tolerant salt time,
Improve appraisal cost, and due to the impact of various factors, measurement error is bigger, it is difficult to confirm the accuracy of result, the most very
Whether person's index can be used in Salt-Tolerance Identification all needs to be investigated.
The present invention is directed to the saline-alkali environment that Xinjiang is complicated, the method using indoor water planting secondary Saline Alkali Stress Resistance Identification,
Phenotypic character, Biomass and the SPAD value of chlorophyll content (hand-held chlorophyll meter mensuration) that investigation salt tolerance is relevant, identifies that half is wild
The resistance level of raw cotton material.Adopting this method, not only authentication method is easy and simple to handle, and qualification result is reliable, resistance is steady
Fixed, not only substantially uniform with single salt stress (NaCl) resistance level, and with the resistance under Saline Alkali Stress in actual production
Level is consistent, thus more puts into practice value and meaning.
Summary of the invention
The method that it is an object of the invention to provide semi-wild cotton secondary saline and alkaline Resistance Identification in seedling stage.
Secondary saline and alkaline Resistance Identification method is comprised the following steps seedling stage by the semi-wild cotton according to the present invention:
(1) seed preparation: cleaning and sterilizing, Dormancy breaking.Then at one osculum of hole of bead end-grain cutting, radicle is not injured, standby；
(2) germination；
(3) transplant seedlings water planting: after germination 3d, select the consistent seedling replanting of growing way and carry out water planting；
(4) Stress treatment: cultivate to tri-leaf period, selects the consistent seedling of growing way and carries out the training of secondary saline solution Stress treatment
Support and the Hoagland solution control of 1/2 concentration process cultivation,
Wherein, the formula of described secondary saline solution is: 0.0086mol/L NaHCO3、0.1411mol/L Na2SO4、
0.0097mol/L K2SO4、0.0583mol/L MgSO4·7H2O、0.0970mol/L CaCl2, pH=7.5；
(5) character investigation: secondary Saline Alkali Stress process 48h time investigation root length, stem length, root fresh weight, root dry weight, stem fresh weight,
Stem weight, leaf fresh weight, leaf dry weight, chlorophyll relative value, each character weight is respectively 0.08,0.14,0.13,0.12,0.11,
0.11、0.11、0.09、0.12；
(6) evaluation of resistance: according to survey result, calculates the comprehensive ST of resistance to salt figure of each material, evaluates semi-wild cotton seedling stage
Secondary saline and alkaline resistance,
ST=Σ (WiRi), Wi is the weight of i index, and Ri is the relative value of i index
ST > 0.8 is Salt-tolerant Materials, and ST≤0.8 is salt density value material, and the comprehensive the biggest salt tolerance of resistance to salt figure is the strongest, is worth the least
Material is the most sensitive to salt.
According to the detailed description of the invention of the present invention, secondary saline and alkaline Resistance Identification method is included seedling stage by described semi-wild cotton
Following steps:
(1) seed preparation: the seed of chosen material, through concentrated sulphuric acid (98%) lint, after clear water is cleaned, uses 5% hypochlorous acid
Sodium is sterilized 10 minutes, and clear water is cleaned and dried, and is placed in 40 DEG C of baking oven 24h Dormancy breaking.Then plumpness, color and luster are selected consistent
Seed, at one osculum of hole of bead end-grain cutting, does not injure radicle, standby.
(2) germination: using three metafiltration paper rolls to germinate, seed is uniformly placed in double-layer filter paper side, then by the 3rd
Filter paper is bedded in the double-layer filter paper putting seed, add suitable quantity of water moistening after, three metafiltration paper are folded into filter paper volume, are placed in
In tank, seed end is upper, and tank middle water level about reaches the 1/3 of filter paper volume height.Shading 3d, ambient temperature 28 DEG C, humidity
85%.
(3) transplant seedlings water planting: after germination 3d, selecting the consistent seedling replanting of growing way and carry out water planting, clear water cultivates about 5d, first
When true leaf grows, it is changed to the Hoagland Solution culture method of 1/2 concentration, then changes the Hoagland battalion of 1/2 concentration every 3d
Nutrient solution, ambient temperature 28 DEG C, humidity 85%, 16/8h illumination.In incubation, note picking up except dead Seedling and selecting replacing growing way
Consistent seedling.
(4) Stress treatment: cultivate to tri-leaf period, about 20d, selects the consistent seedling of growing way and carries out secondary saline solution and coerce
Process and cultivate and the Hoagland solution control process cultivation of 1/2 concentration.
Secondary saline solution formula: 0.0086mol/L NaHCO3、0.1411mol/L Na2SO4、0.0097mol/L
K2SO4、0.0583mol/L MgSO4·7H2O、0.0970mol/L CaCl2, pH=7.5.
(5) character investigation: investigation root length (RL), stem length (SL), root fresh weight (RFW), root when secondary Saline Alkali Stress processes 48h
Dry weight (RDW), stem fresh weight (SFW), stem weight (SDW), leaf fresh weight (LFW), leaf dry weight (LDW), chlorophyll relative value (CHC),
Each character weight is respectively 0.08,0.14,0.13,0.12,0.11,0.11,0.11,0.09,0.12.
(6) evaluation of resistance: according to survey result, calculates the comprehensive resistance to salt figure (ST) of each material, evaluates semi-wild cotton Seedling
Phase secondary saline and alkaline resistance.
ST=Σ (WiRi), Wi is the weight of i index, and Ri is the relative value of i index
ST > 0.8 is Salt-tolerant Materials, and ST≤0.8 is salt density value material, and the comprehensive the biggest salt tolerance of resistance to salt figure is the strongest, is worth the least
Material is the most sensitive to salt.
Existing cotton in seedling stage water planting Salt And Alkali Tolerance is identified, does not has the concrete technical side of lab simulation complicated soil salt and alkali component
Method.The present invention simulates double wild cotton of secondary saline-alkali environment, and to carry out Tolerant salt technical method the most perfect, and can be real
Judge the secondary saline and alkaline resistance that semi-wild is cotton.The present invention improves existing Salt Tolerance at Seedling Stage authentication method, eliminates outside Saline Alkali Stress environment
The impact on result of other factors, step (3) is constantly selected the consistent seedling of growing way, rejecting abnormalities seedling is identified, step
Suddenly (6) use index relative value to identify.In step (4), the formula of salt and alkali component is simulation Xinjiang secondary salinization of land soil
Ion component, the method design identified in conjunction with indoor water planting, this formula, for use first, makes up the improvement saline and alkaline qualification of water planting in seedling stage
In deficiency.In step (5), the indicator combination of investigation is for use first, improves evaluation index in Salt And Alkali Tolerance is identified and selects many
Sample, the complexity of mensuration, coordinate this set index to use, it is possible to the cotton saline and alkaline resistance of characterization and evaluation semi-wild accurately, even
Can use in other Cotton Gossypii germline Salt And Alkali Tolerances are identified.Step (6) passes through comprehensive resistance to salt figure (ST), double this Sal of wild cotton
Alkali resistance carries out classification, improves the ambiguity of Saline alkali tolerance characterization and evaluation, subjectivity.The present invention is with digitized comprehensive salt tolerant
Value carries out classification to Saline alkali tolerance, and result is the most objective, accurate.
It is an advantage of the current invention that:
1, simple operating steps and scientific and reasonable, during seed preparation to Stress treatment, constantly selects consistent kind
Son or seedling, decrease the interference of other factors, compensate in Tolerant salt technology thinking little of this factor, or be limited to technology
The deficiency of the interference of this factor can not be got rid of.
2, the method is first in the secondary saline and alkaline resistance that qualification semi-wild in seedling stage in indoor is cotton, and qualification is not subject to seasonal restrictions,
Do not affected by outside climatic；Qualification cycle is short, land for growing field crops cotton in seedling stage Tolerant salt about 50d, and indoor water planting is identified and only needed
About 25d.
Step (4) 3, uses ciltivating process simulation Kurle region, Xinjiang secondary salinization of land growing environment, water planting first
Kurle region, secondary saline and alkaline compound basis Xinjiang secondary salinization of land ion composition and water planting condition design, stressful environmental and Xinjiang
Kurle region secondary salinization of land environment is similar to, and supplements the deficiency in existing water planting Tolerant salt technology.Experiment proves that, identify
Qualification result during result is identified with land for growing field crops is consistent, and conclusion utilizes the most aborning.
4, numerous and diverse to investigation index in Tolerant salt technology in step (5), that needed for measurement index, various resources are many shortcoming
Improved.Selected investigation index is scientific and reasonable, is cotton seedling physiological and biochemical activity under secondary Saline Alkali Stress
Final performance, manpower and materials are few, the time is short, measuring method is simple, data are accurate needed for investigation measurement index.The weight of index
By confirming the related data of 6 parts of materials of resistance in land for growing field crops and indoor qualification, with the entitled methods analyst of principal component analysis
Gained, the statistical method science used, reliable, the weight of each index of gained, and can be in semi-wild for use first
The secondary saline and alkaline Resistance Identification of cotton, even other germline cotton seedlings uses.The comprehensive resistance to salt figure of gained is accurate, objective, visual
Change, it is possible to judge the secondary saline and alkaline resistance of cotton seedling, compare existing Tolerant salt technology, simplify Tolerant salt data
Process and interpretation of result.
Accompanying drawing explanation
Fig. 1 water planting complex salt alkaline stress 48h later half wild cotton growing state: a-1, a-2, Mary adds Lang Te 85, b-1, b-
2, broad-leaved cotton 32, c-1, c-2, CCRI 16, d-1, d-2, CCRI 12, e-1, e-2, broad-leaved cotton 40, f-1, f-2, broad-leaved is cotton
130, wherein, 1, nutritional solution processes.2, secondary Saline Alkali Stress processes.
Detailed description of the invention
Embodiment 1 uses semi-wild cotton secondary saline and alkaline Resistance Identification method in seedling stage anti-through repeatedly screen to big Tanaka
The resistance level of 4 parts of stable semi-wild cotton materials of perception and 2 parts of Gossypium hirsutum L. conventional cultivation kinds is identified.
1 test material
4 parts of semi-wild cottons originate in Mexico, by the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute 2007 quoted from American National
Cotton Germplasms storehouse.2 parts of Gossypium hirsutum L. conventional cultivation kinds.6 parts of test material resistance levels are known.
Table 1 test material and resistance level thereof
2 experimental techniques
1) seed preparation: the seed of used material, after concentrated sulphuric acid (98%) lint, cleans and dries, be placed in 40 DEG C of bakings
Case 24h Dormancy breaking, then selects the seed that plumpness, color and luster are consistent, at one osculum of hole of bead end-grain cutting, does not injure radicle, standby
With.
2) germination: using three metafiltration paper rolls to germinate, seed is uniformly placed in double-layer filter paper side, then by the 3rd
Filter paper is bedded in the double-layer filter paper putting seed, add suitable quantity of water moistening after, three metafiltration paper are folded into filter paper volume, are placed in water
In groove, seed end is upper, and tank middle water level about reaches the 1/3 of filter paper volume height.Shading 3d, ambient temperature 28 DEG C, humidity 85%.
3) transplant seedlings water planting: after germination 3d, select the consistent seedling replanting of growing way and carry out water planting.Water planting use 30*20*10's is black
Color (shading) box, every box 5L clear water, plant 12 strain seedling, ambient temperature 28 DEG C, humidity 85%, 16/8h illumination.Cultivate about
5d, when rough leaf grows, is changed to the Hoagland Solution culture method of 1/2, then changes the Hoagland battalion of 1/2 every 3d
Nutrient solution, solution is 5L/ box.
Hoagland ' s nutrient solution prescription: 945mg/L calcium nitrate, 607mg/L potassium nitrate, 115mg/L ammonium phosphate, 493mg/
L magnesium sulfate, 2.5ml/L iron salt solutions, 5ml/L trace element, pH=6.0.
Iron salt solutions: ferrous sulfate heptahydrate 2.78g, disodiumedetate 3.73g, distilled water 500ml
Liquid microelement: potassium iodide 0.83mg/l, boric acid 6.2mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, molybdenum
Acid sodium 0.25mg/L, copper sulfate 0.025mg/L, cobaltous chloride 0.025mg/L
4) Stress treatment: cultivate about 20d, when the 3rd true leaf grows, selects the consistent seedling of growing way and carries out secondary saline and alkaline
Solution Stress treatment is cultivated and the Hoagland solution control of 1/2 concentration processes cultivation, and solution is 5L/ box.
Secondary saline solution formula: 0.1411mol/L Na2SO4、0.0097mol/L K2SO4、0.0583mol/L
MgSO4·7H2O、0.0970mol/L CaCl2、0.0086mol/L NaHCO3, pH=7.5.
5) character investigation: investigation root length (RL), stem length (SL), root fresh weight (RFW), root when secondary Saline Alkali Stress processes 48h
Dry weight (RDW), stem fresh weight (SFW), stem weight (SDW), leaf fresh weight (LFW), leaf dry weight (LDW), chlorophyll relative value (CHC).
6) according to survey result, semi-wild cotton secondary saline and alkaline resistance in seedling stage is evaluated.
29 index relative value of table and comprehensive resistance to salt figure
Broad-leaved cotton 130, broad-leaved cotton 32, broad-leaved cotton 40, Mary add the comprehensive salt tolerant of Lang Te 85, CCRI 12, CCRI 16
Value is 0.69,0.86,0.79,1.41,0.8,0.81 respectively, the most comprehensive resistance to salt figure more than 0.8 CCRI 16, Mary add youth
Spy 85 and broad-leaved cotton 32 are Salt-tolerant Materials；Comprehensive resistance to salt figure CCRI 12, broad-leaved cotton 40 and broad-leaved cotton 130 less than or equal to 0.8
For salt density value material.The order of 6 Cotton Varieties at Seeding Stage salt tolerances is Mary Jia Langte 85 > broad-leaved cotton 32 > CCRI 16 > in 12
> broad-leaved cotton 40 > broad-leaved cotton 130.The Mary wherein having specific resistivity comparison cultigen CCRI 16 resistance adds Lang Te 85, broad-leaved cotton
32 and the broad-leaved cotton 130 more weak than sensitive control cultigen CCRI 12 resistance, broad-leaved cotton 40.Use this method to 6 parts of materials
Being identified, acquired results is consistent with its field resistance level, and is sorted its resistance power.Result accurately,
Objective, visualization.
The feasibility of the method is verified, can double wild cotton material even other germline Cotton Gossypii according to this method
The secondary saline and alkaline resistance in seedling stage of material is identified, it is also possible to secondary saline and alkaline anti-for seedling stage according to double wild cotton material of test demand
Property power is ranked up.
Claims (2)
1. semi-wild cotton seedling stage is to secondary saline and alkaline Resistance Identification method, it is characterised in that said method comprising the steps of:
(1) seed preparation: cleaning and sterilizing, Dormancy breaking.Then at one osculum of hole of bead end-grain cutting, radicle is not injured, standby；
(2) germination；
(3) transplant seedlings water planting: after germination 3d, select the consistent seedling replanting of growing way and carry out water planting；
(4) Stress treatment: cultivate to tri-leaf period, select the consistent seedling of growing way carry out secondary saline solution Stress treatment cultivate and
The Hoagland solution control of 1/2 concentration processes to be cultivated,
Wherein, the formula of described secondary saline solution is: 0.0086mol/L NaHCO3、0.1411mol/L Na2SO4、
0.0097mol/L K2SO4、0.0583mol/L MgSO4·7H2O、0.0970mol/L CaCl2, pH=7.5；
(5) character investigation: investigation root length, stem length, root fresh weight, root dry weight, stem fresh weight, stem when secondary Saline Alkali Stress processes 48h
Weight, leaf fresh weight, leaf dry weight, chlorophyll relative value, each character weight is respectively 0.08,0.14,0.13,0.12,0.11,
0.11、0.11、0.09、0.12；
(6) evaluation of resistance: according to survey result, calculates the comprehensive ST of resistance to salt figure of each material, evaluates semi-wild cotton secondary for seedling stage
Saline and alkaline resistance,
ST=Σ (WiRi), Wi is the weight of i index, and Ri is the relative value of i index
ST > 0.8 is Salt-tolerant Materials, and ST≤0.8 is salt density value material, and the comprehensive the biggest salt tolerance of resistance to salt figure is the strongest, is worth the least material
The most sensitive to salt.
Semi-wild the most according to claim 1 cotton seedling stage is to secondary saline and alkaline Resistance Identification method, it is characterised in that in step
Suddenly in (3), clear water cultivates 5d, when rough leaf grows, is changed to the Hoagland Solution culture method of 1/2 concentration, then every
3d changes the Hoagland nutritional solution of 1/2 concentration, ambient temperature 28 DEG C, humidity 85%, 16/8h illumination, in incubation, picks up
Except dead Seedling and select replacing the consistent seedling of growing way.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610530778.2A CN106069696B (en) | 2016-07-07 | 2016-07-07 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610530778.2A CN106069696B (en) | 2016-07-07 | 2016-07-07 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106069696A true CN106069696A (en) | 2016-11-09 |
| CN106069696B CN106069696B (en) | 2019-05-17 |
Family
ID=57213390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610530778.2A Active CN106069696B (en) | 2016-07-07 | 2016-07-07 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106069696B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136418A (en) * | 2017-05-31 | 2017-09-08 | 福建农林大学 | A kind of production technology of Job's tears seedling powder |
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
| CN111771702A (en) * | 2020-07-27 | 2020-10-16 | 黑龙江省科学院大庆分院 | Method for evaluating saline-alkali resistance of industrial hemp in seedling stage by using water culture method |
| CN113133390A (en) * | 2021-04-14 | 2021-07-20 | 陕西国际商贸学院 | Method for testing influence of double stress on growth physiological characteristics of gentiana straminea maxim |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860159A (en) * | 2012-10-10 | 2013-01-09 | 江苏省农业科学院 | Salt-tolerance determining method for barley at seedling stage |
| CN103704103A (en) * | 2013-12-20 | 2014-04-09 | 德州市农业科学研究院 | Soybean sprout stage sand culture salt tolerance identification method |
| CN103869058A (en) * | 2014-03-21 | 2014-06-18 | 上海交通大学 | Rapid evaluation method for salt tolerance of tomatoes |
| CN103875469A (en) * | 2012-12-20 | 2014-06-25 | 盐城工学院 | Method for quickly evaluating salt tolerance of grapes |
| CN104429933A (en) * | 2014-09-22 | 2015-03-25 | 中国农业科学院棉花研究所 | Method for screening semi-wild cotton material with high salt and alkali resistance |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
| CN105325192A (en) * | 2015-11-16 | 2016-02-17 | 江苏省农业科学院 | Identification method of salt-tolerant resource in seedling period of cowpea |
-
2016
- 2016-07-07 CN CN201610530778.2A patent/CN106069696B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860159A (en) * | 2012-10-10 | 2013-01-09 | 江苏省农业科学院 | Salt-tolerance determining method for barley at seedling stage |
| CN103875469A (en) * | 2012-12-20 | 2014-06-25 | 盐城工学院 | Method for quickly evaluating salt tolerance of grapes |
| CN103704103A (en) * | 2013-12-20 | 2014-04-09 | 德州市农业科学研究院 | Soybean sprout stage sand culture salt tolerance identification method |
| CN103869058A (en) * | 2014-03-21 | 2014-06-18 | 上海交通大学 | Rapid evaluation method for salt tolerance of tomatoes |
| CN104429933A (en) * | 2014-09-22 | 2015-03-25 | 中国农业科学院棉花研究所 | Method for screening semi-wild cotton material with high salt and alkali resistance |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
| CN105325192A (en) * | 2015-11-16 | 2016-02-17 | 江苏省农业科学院 | Identification method of salt-tolerant resource in seedling period of cowpea |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136418A (en) * | 2017-05-31 | 2017-09-08 | 福建农林大学 | A kind of production technology of Job's tears seedling powder |
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
| CN109874665B (en) * | 2019-04-11 | 2022-06-14 | 新疆农业科学院经济作物研究所 | Breeding method of salt-tolerant cotton variety based on rapid embryo seedling establishment |
| CN111771702A (en) * | 2020-07-27 | 2020-10-16 | 黑龙江省科学院大庆分院 | Method for evaluating saline-alkali resistance of industrial hemp in seedling stage by using water culture method |
| CN113133390A (en) * | 2021-04-14 | 2021-07-20 | 陕西国际商贸学院 | Method for testing influence of double stress on growth physiological characteristics of gentiana straminea maxim |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106069696B (en) | 2019-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhou et al. | Drought‐induced changes in root biomass largely result from altered root morphological traits: Evidence from a synthesis of global field trials | |
| Wen et al. | Tradeoffs among root morphology, exudation and mycorrhizal symbioses for phosphorus‐acquisition strategies of 16 crop species | |
| Pellegrino et al. | Impact on soil quality of a 10-year-old short-rotation coppice poplar stand compared with intensive agricultural and uncultivated systems in a Mediterranean area | |
| Xue et al. | A new method for soil health assessment based on Analytic Hierarchy Process and meta-analysis | |
| Garnier et al. | A trait-based approach to comparative functional plant ecology: concepts, methods and applications for agroecology. A review | |
| Bayuelo-Jiménez et al. | Genotypic variation for root traits of maize (Zea mays L.) from the Purhepecha Plateau under contrasting phosphorus availability | |
| Jarwar et al. | Genetic divergence on the basis of principal component, correlation and cluster analysis of yield and quality traits in cotton cultivars | |
| CN106069696A (en) | Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage | |
| Li et al. | Mixed‐cropping systems of different rice cultivars have grain yield and quality advantages over mono‐cropping systems | |
| Heredia et al. | Breeding rice for a changing climate by improving adaptations to water saving technologies | |
| Sharififar | Assessment of different methods of soil suitability classification for wheat cultivation. | |
| Tepe et al. | Comparison of some winter lentil cultivars in weed–crop competition | |
| Fenta et al. | Role of fixing nitrogen in common bean growth under water deficit conditions | |
| Singh et al. | Variation of aluminium tolerance in lentil (Lens culinaris Medik.) | |
| Jia et al. | Screening and comprehensive evaluation of rice (Oryza sativa L. subsp. japonica Kato) germplasm resources for nitrogen efficiency in Xinjiang, China | |
| Fu et al. | Quantifying key internal and external yield-limiting factors for Chinese pear in smallholder dominant areas | |
| Wu et al. | Effects of nitrogen fertilization on chlorophyll fluorescence change in maize (Zea mays L.) under waterlogging at seedling stage | |
| Wang et al. | Large-scale screening for Aegilops tauschii tolerant genotypes to phosphorus deficiency at seedling stage | |
| McCauley | Nitrogen fixation by annual legume green manures in a semi-arid cropping system | |
| Kakade et al. | Differential response of root morphology of rice (Oryza sativa L.) genotypes under different phosphorus conditions | |
| Stojšin et al. | Utilizing the Stability of Yield Parameters as a Technique to Select Salinity-Tolerant Wheat Genotypes | |
| Chantarachot et al. | Improvement of growth and phosphorus utilization efficiency in Thai rice (Oryza sativa L. ssp indica cv. Chaew Khing) by inoculation of arbuscular mycorrhizal fungi under high phosphorus supply. | |
| Duan et al. | Changes in dominant weeds of wheat in a rice–wheat rotation system as affected by composted manure and straw amendments | |
| Ozdemir et al. | Line x Tester analysis of stomatal conductance, chlorophyll content, photosynthetic efficiency, and transpiration rate traits in maize | |
| Bernas et al. | Greenhouse gasses emissions during maize growing for energy purposes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |