CN105210861A - A kind of cotton seeds selection of salt tolerance method - Google Patents
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Abstract
The invention discloses a kind of cotton seeds selection of salt tolerance method, comprise the steps: after cotton seeds process, with the artificial seawater nutrient solution of variable concentrations, filter after seed soaking; Be placed in culture dish, add artificial seawater nutrient solution, every day records germinative number, and calculates germination vigor, germination index and vitality index; By clean for the root distilled water flushing of the cotton seeds after germination, cultivate in the shading-glass bottle dividing individual plant immigration that the artificial seawater nutrient solution of variable concentrations is housed, and timing every day supplements corresponding artificial seawater nutrient solution in cotton seedling whole root immersion culture fluid; Within 8-11 days, take out the fibrous root quantity of checking every strain cotton; Measure after 20d that the Seedling Height of each processed group, stem number, stem are thick, root Fresh Yuxincao, Root dry weight and root volume carry out data and calculate assessment.The present invention, by ciltivating process, to its bud phase and economical character index multi-variate statistical analysis in seedling stage, thus has entered comprehensive identification evaluation to the salt resistance of cotton.
Description
Technical field
The present invention relates to plant physiology field, be specifically related to a kind of cotton seeds selection of salt tolerance method.
Background technology
Relatively stable relevant not only with osmotic adjustment ability and Protective substances of the salt resistance of cotton, more mainly depends on that root system is to the Selective absorber of salinity and the compartmentalization at organ, tissue and cell three levels thereof.If the salt ion absorbed can morely stay in root, reduce the transport to overground part and distribution, then can show stronger Salt-endurance.Simultaneously because the Saline alkali tolerance of plant is by controlled by multiple genes, resistance mechanism more complicated, and root system is that cotton is injured the earliest in saline and alkaline adverse circumstance, the most direct position, so the fresh weight of root system, dry weight, root are long, radical is all morphological index important in Salt And Alkali Tolerance qualification, simultaneously, the change of quantity to salt concentration of coring is the most responsive, and therefore it can be used as the ideal indicator of the Forepart identification of Salt And Alkali Tolerance cotton Forepart identification system and screening this system applicable.
For a long time, the screening of antagonism/Salt And Alkali Tolerance cotton material or qualification are all adopt saline land, field, artificial salt pond to test, or the parameter of some Physiology and biochemistries of indoor measurement, as comparing reference.Existing method carries out salt damage according to cotton material upgrowth situation in saline land or salt pond to check and carry out judging whether to have anti-/ patience to saline and alkaline.Although the method is directly at field test, qualification result is relatively direct, and annual cotton only has a season of growth, namely can only carry out a collection of test every year; To higher required by verification field, as salinity will have certain gradient, salt pond or place, saline land cannot be changed, because be not available to through the saline land of this kind of approval test or salt pond plant other plant, in case bring salt damage to other crops, floor space is large, and determination rates is low; Salt damage symptom is individual scope qualitatively, does not have concrete digital quantization, and the result difference that different testing persons observes is comparatively large, and the conclusion drawn has difference and instability; Saline land, field is nature, and the not easily unification of the degree of mixedness of soil and saline and alkaline, although the salinity in salt pond is the NaCl be manually sprinkled into, but after entering soil, the factors such as the content of soil, pH value and weather rainfall all can cause the change at any time of salinity level, between year and parallel between the comparativity of result of the test poor.
Water planting is the one of soilless culture, it is a new and high technology in modern agriculture science and technology, because it instead of soil environment with the root system environment of artificial creation, because it has many advantages in Tolerant salt, it provide a homogeneous root system ecotope especially, thus the reaction of plant to salt damage can be gone out by Accurate Determining, so be widely applied in plant salt tolerance qualification.
Summary of the invention
For solving the problem, the invention provides a kind of cotton seeds selection of salt tolerance method, by ciltivating process, to its bud phase and economical character index multi-variate statistical analysis in seedling stage, thus comprehensive identification evaluation having been entered to the salt resistance of cotton.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of cotton seeds selection of salt tolerance method, comprises the steps:
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, adopt carbendazim spraying can kill bulbil and the mycelia of attachment the surface of the seed, distilled water flushing 3 times; With 1/2Hoagland complete nutrition liquid for solution, mix the artificial seawater nutrient solution being mixed with 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% respectively with artificial seawater, filter after seed soaking 48h; Be placed in be covered with 2 metafiltration paper culture dish in, every ware adds artificial seawater nutrient solution 10mL, vernalization at 28 DEG C, and every day records germinative number, and calculates germination vigor, germination index cleaning seed change artificial seawater nutrient solution;
Meanwhile, adopt glass vertical panel sproutung method to measure plumular axis and radicle long, calculating vitality index; Each process repetition 3 times,
S2, by clean for the root distilled water flushing of cotton seeds after germinateing, dividing individual plant to move into is equipped with in the shading-glass bottle of artificial seawater nutrient solution of 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt%, cotton cotyledon is stayed outside bottleneck, be placed in 23 scholar 2 DEG C, light intensity 2000lx, cultivate under photoperiod 12/12h condition, in vial, timing every day supplements corresponding artificial seawater nutrient solution in cotton seedling whole root immersion culture fluid;
Within S3,8-11 days, take out the radical amount of checking every strain cotton, comprise the fibrous root quantity on taproot and lateral root and side root;
After S4, process 20d, measure that the Seedling Height of each processed group, stem number, stem are thick respectively, root Fresh Yuxincao, Root dry weight and root volume;
S5, data preparation, calculating mean value, and according to the salt tolerant efficiency α of each single index of following formulae discovery:
α=processed group measured value/control group measured value
Calculate the comprehensive D of resistance to salt figure of each kind according to the following formula:
D＝X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+X7*0.643+X8*0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent that germination vigor, germination index, coring quantity, radicle length, plant height, stem number, stem are thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume respectively;
S6, judge the salt resistance of kind according to the size of comprehensive resistance to salt figure, D value is larger, and the salt damage be subject to is less, and the salt resistance of kind is stronger; D value is less, and the salt damage be subject to is larger, and the salt resistance of kind is more weak.
Wherein, described artificial seawater often rises and comprises 26.726 grams, sodium chloride, 2.26 grams, magnesium chloride, 3.248 grams, magnesium sulfate, 1.153 grams, calcium chloride, sodium bicarbonate 0.198 gram, 0.721 gram, potassium chloride.
The present invention has following beneficial effect:
Cotton plants is cultivated by utilizing ciltivating process, in nutrient solution, field arranges different salinity gradients, transplanted by specific seedling raising manners and specific time, and check specific parameter in the specific time, according to specific, can directly observe, simple parameter carries out judging the resistance level of this cotton material, simultaneously to its bud phase and economical character index multi-variate statistical analysis in seedling stage, thus comprehensive identification evaluation is entered to the salt resistance of cotton.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The artificial seawater used in following examples often rises and comprises 26.726 grams, sodium chloride, 2.26 grams, magnesium chloride, 3.248 grams, magnesium sulfate, 1.153 grams, calcium chloride, sodium bicarbonate 0.198 gram, 0.721 gram, potassium chloride.
Embodiment
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, adopt carbendazim spraying can kill bulbil and the mycelia of attachment the surface of the seed, distilled water flushing 3 times; With 1/2Hoagland complete nutrition liquid for solution, mix the artificial seawater nutrient solution being mixed with 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% respectively with artificial seawater, filter after seed soaking 48h; Be placed in be covered with 2 metafiltration paper culture dish in, every ware adds artificial seawater nutrient solution 10mL, vernalization at 28 DEG C, and every day records germinative number, and calculates germination vigor, germination index cleaning seed change artificial seawater nutrient solution;
Meanwhile, adopt glass vertical panel sproutung method to measure plumular axis and radicle long, calculating vitality index; Each process repetition 3 times,
S2, by clean for the root distilled water flushing of cotton seeds after germinateing, dividing individual plant to move into is equipped with in the shading-glass bottle of artificial seawater nutrient solution of 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt%, cotton cotyledon is stayed outside bottleneck, be placed in 23 scholar 2 DEG C, light intensity 2000lx, cultivate under photoperiod 12/12h condition, in vial, timing every day supplements corresponding artificial seawater nutrient solution in cotton seedling whole root immersion culture fluid;
Within S3,8-11 days, take out the radical amount of checking every strain cotton, comprise the fibrous root quantity on taproot and lateral root and side root;
After S4, process 20d, measure that the Seedling Height of each processed group, stem number, stem are thick respectively, root Fresh Yuxincao, Root dry weight and root volume;
S5, data preparation, calculating mean value, and according to the salt tolerant efficiency α of each single index of following formulae discovery:
α=processed group measured value/control group measured value
Calculate the comprehensive D of resistance to salt figure of each kind according to the following formula:
D＝X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+X7*0.643+X8*0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent that germination vigor, germination index, coring quantity, radicle length, plant height, stem number, stem are thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume respectively;
S6, judge the salt resistance of kind according to the size of comprehensive resistance to salt figure, D value is larger, and the salt damage be subject to is less, and the salt resistance of kind is stronger; D value is less, and the salt damage be subject to is larger, and the salt resistance of kind is more weak.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. a cotton seeds selection of salt tolerance method, is characterized in that, comprises the steps:
S1, selection maturity and cotton seeds of the same size, except mould impurity elimination, adopt carbendazim spraying can kill bulbil and the mycelia of attachment the surface of the seed, distilled water flushing 3 times; With 1/2Hoagland complete nutrition liquid for solution, mix the artificial seawater nutrient solution being mixed with 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt% respectively with artificial seawater, filter after seed soaking 48h; Be placed in be covered with 2 metafiltration paper culture dish in, every ware adds artificial seawater nutrient solution 10mL, vernalization at 28 DEG C, and every day records germinative number, and calculates germination vigor, germination index cleaning seed change artificial seawater nutrient solution;
Meanwhile, adopt glass vertical panel sproutung method to measure plumular axis and radicle long, calculating vitality index; Each process repetition 3 times,
S2, by clean for the root distilled water flushing of cotton seeds after germinateing, dividing individual plant to move into is equipped with in the shading-glass bottle of artificial seawater nutrient solution of 0.0wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt% and 0.8wt%, cotton cotyledon is stayed outside bottleneck, be placed in 23 scholar 2 DEG C, light intensity 2000lx, cultivate under photoperiod 12/12h condition, in vial, timing every day supplements corresponding artificial seawater nutrient solution in cotton seedling whole root immersion culture fluid;
Within S3,8-11 days, take out the radical amount of checking every strain cotton, comprise the fibrous root quantity on taproot and lateral root and side root;
After S4, process 20d, measure that the Seedling Height of each processed group, stem number, stem are thick respectively, root Fresh Yuxincao, Root dry weight and root volume;
S5, data preparation, calculating mean value, and according to the salt tolerant efficiency α of each single index of following formulae discovery:
α=processed group measured value/control group measured value
Calculate the comprehensive D of resistance to salt figure of each kind according to the following formula:
D＝X1*0.903+X2*0.906+X3*0.789+X4*0.961+X5*0.621+X6*0.831+X7*0.643+X8*0.958+X9*0.945+X10*0.935+X11*0.906
Wherein, X1, X2, X3, X4, X5, X6, X7, X8, X9, X10, X11 represent that germination vigor, germination index, coring quantity, radicle length, plant height, stem number, stem are thick, the salt tolerant efficiency of vitality index, root Fresh Yuxincao, Root dry weight and root volume respectively;
S6, judge the salt resistance of kind according to the size of comprehensive resistance to salt figure, D value is larger, and the salt damage be subject to is less, and the salt resistance of kind is stronger; D value is less, and the salt damage be subject to is larger, and the salt resistance of kind is more weak.
2. a kind of cotton seeds selection of salt tolerance method according to claim 1, it is characterized in that, described artificial seawater often rises and comprises 26.726 grams, sodium chloride, 2.26 grams, magnesium chloride, 3.248 grams, magnesium sulfate, 1.153 grams, calcium chloride, sodium bicarbonate 0.198 gram, 0.721 gram, potassium chloride.
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Cited By (11)
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| CN105850274A (en) * | 2016-04-20 | 2016-08-17 | 运城学院 | Method for screening salt-resistant cotton varieties |
| CN105874967A (en) * | 2016-04-20 | 2016-08-24 | 运城学院 | Method for identifying salt-stressed resistance in cotton seeds |
| CN105900636A (en) * | 2016-04-20 | 2016-08-31 | 运城学院 | Method for screening salt-tolerance kidney bean variety |
| CN106069696A (en) * | 2016-07-07 | 2016-11-09 | 中国农业科学院棉花研究所 | Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage |
| CN106613826A (en) * | 2016-11-09 | 2017-05-10 | 中国农业科学院棉花研究所 | Low-potassium-tolerant cotton seedling nutrient solution and application thereof |
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
| CN111247898A (en) * | 2020-01-16 | 2020-06-09 | 邯郸市农业科学院 | Screening method of storage-resistant wheat seeds |
| CN112273222A (en) * | 2020-10-22 | 2021-01-29 | 河北省农林科学院旱作农业研究所 | Cotton variety breeding method |
| CN114532221A (en) * | 2022-03-10 | 2022-05-27 | 河北省农林科学院旱作农业研究所 | Method for breeding salt-tolerant cotton variety by using natural seawater |
| CN116058130A (en) * | 2023-04-06 | 2023-05-05 | 安徽中青检验检测有限公司 | Method and device for detecting seed vitality |
| CN117929248A (en) * | 2024-01-25 | 2024-04-26 | 新疆农业科学院经济作物研究所(新疆维吾尔自治区棉花研究所新疆维吾尔自治区甜菜改良中心) | Method and system for identifying salt-tolerant alkalinity of rape in whole growth period based on saline-alkali soil |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105850274A (en) * | 2016-04-20 | 2016-08-17 | 运城学院 | Method for screening salt-resistant cotton varieties |
| CN105874967A (en) * | 2016-04-20 | 2016-08-24 | 运城学院 | Method for identifying salt-stressed resistance in cotton seeds |
| CN105900636A (en) * | 2016-04-20 | 2016-08-31 | 运城学院 | Method for screening salt-tolerance kidney bean variety |
| CN105874967B (en) * | 2016-04-20 | 2018-08-14 | 运城学院 | A kind of method of cotton seeds to the Resistance Identification of salt stress |
| CN105850274B (en) * | 2016-04-20 | 2018-09-25 | 运城学院 | A method of screening salt tolerant cotton variety |
| CN106069696A (en) * | 2016-07-07 | 2016-11-09 | 中国农业科学院棉花研究所 | Semi-wild is cotton to water planting Resistance Identification method in secondary saline and alkaline seedling stage |
| CN106069696B (en) * | 2016-07-07 | 2019-05-17 | 中国农业科学院棉花研究所 | Semi-wild cotton is to secondary saline and alkaline seedling stage water planting Resistance Identification method |
| CN106613826A (en) * | 2016-11-09 | 2017-05-10 | 中国农业科学院棉花研究所 | Low-potassium-tolerant cotton seedling nutrient solution and application thereof |
| CN109874665A (en) * | 2019-04-11 | 2019-06-14 | 新疆农业科学院经济作物研究所 | A kind of selection of the salt tolerant cotton variety based on embryo sprouting and rooting |
| CN109874665B (en) * | 2019-04-11 | 2022-06-14 | 新疆农业科学院经济作物研究所 | Breeding method of salt-tolerant cotton variety based on rapid embryo seedling establishment |
| CN111247898A (en) * | 2020-01-16 | 2020-06-09 | 邯郸市农业科学院 | Screening method of storage-resistant wheat seeds |
| CN112273222A (en) * | 2020-10-22 | 2021-01-29 | 河北省农林科学院旱作农业研究所 | Cotton variety breeding method |
| CN114532221A (en) * | 2022-03-10 | 2022-05-27 | 河北省农林科学院旱作农业研究所 | Method for breeding salt-tolerant cotton variety by using natural seawater |
| CN116058130A (en) * | 2023-04-06 | 2023-05-05 | 安徽中青检验检测有限公司 | Method and device for detecting seed vitality |
| CN117929248A (en) * | 2024-01-25 | 2024-04-26 | 新疆农业科学院经济作物研究所(新疆维吾尔自治区棉花研究所新疆维吾尔自治区甜菜改良中心) | Method and system for identifying salt-tolerant alkalinity of rape in whole growth period based on saline-alkali soil |
| CN117929248B (en) * | 2024-01-25 | 2024-10-01 | 新疆农业科学院经济作物研究所(新疆维吾尔自治区棉花研究所新疆维吾尔自治区甜菜改良中心) | Method and system for identifying salt-tolerant alkalinity of rape in whole growth period based on saline-alkali soil |
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