CN105850274B - A method of screening salt tolerant cotton variety - Google Patents
A method of screening salt tolerant cotton variety Download PDFInfo
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- CN105850274B CN105850274B CN201610248475.1A CN201610248475A CN105850274B CN 105850274 B CN105850274 B CN 105850274B CN 201610248475 A CN201610248475 A CN 201610248475A CN 105850274 B CN105850274 B CN 105850274B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- Botany (AREA)
- Soil Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
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Abstract
The present invention provides a kind of method of screening salt tolerant cotton variety, the cotton seeds of different cultivars are carried out concentrated sulfuric acid lint by the method, and the uniform healthy seed of size, plumpness is then selected after constant temperature drying.It is divided into salt treatment group after sterilizing and blank control group carries out presoaking and germinating, soaking seed, a period of time, each kind chose more growing points crackings, the identical seed of buds length later, it is transplanted respectively into salt treatment aerial fog cultivation turnover box and blank control aerial fog cultivation turnover box and is cultivated according to salt treatment group and blank control group, after culture terminates, the bud length of the germination cotton seeds of salt treatment group and blank control group is measured respectively, the cotton variety more resistant to salt can be filtered out by the analysis comparison of the long data of the bud of cotton seeds.This method cost is not high, easily operated convenient in each local promotion and implementation.
Description
Technical field
The present invention relates to a kind of method of screening cotton variety more particularly to a kind of methods of screening salt tolerant cotton variety.
Background technology
Cotton occupies very important status as the main industrial crops in China in the national economic development.Due to grain
Cotton strives particularly thorny, and the production of cotton is severely impacted.Since cotton itself has saline-alkali tolerant characteristic, development salt can be used as
The pioneer crop of alkali regional economy.And it is the premise of salt tolerant breeding that evaluation and mirror, which select Salinity tolerance germplasm, current salt tolerance of cotton
Identification method mostly heavy workload, it is of high cost, and limited by the season of growth.
For a long time, to the screening or identification of saline-alkali tolerant cotton, planted using field salt-soda soil, artificial salt pond
The parameter of some Physiology and biochemistries of experiment or indoor measurement, as comparison reference.Existing method is according to cotton material in salt-soda soil
Or upgrowth situation progress salt damage investigation is judged whether there is patience to saline and alkaline in salt pond.Although this method is directly in field
Between identify, qualification result is relatively direct, but annual cotton can only carry out a collection of examination every year only there are one the season of growth
It tests；To higher required by verification field, if salinity will have certain gradient, salt pond or salt-soda soil place that can not replace, because through
The salt-soda soil of this kind of qualification test or salt pond is crossed to be not available to plantation other plant and account for prevent bringing salt damage to other crops
Ground area is big, and determination rates are low；And salt damage symptom is a qualitative range, without specific digital quantization, different calibratings
The result difference of personal observations is larger, and the conclusion obtained has difference and unstable；Field salt-soda soil is nature, and soil
The degree of mixedness and saline and alkaline of earth are not easy unification, although the salinity in salt pond is the NaCl being manually sprinkled into, but after entrance soil, soil
The factors such as ingredient, pH value and weather rainfall can cause the variation at any time of salinity level, knot is tested between year and between parallel
The comparativity of fruit is poor.
Patent CN103843491B is disclosed through presoaking and germinating, with seed growth point whether crack and (spit bud) for judge according to
According to judging cotton variety salt tolerant situation by spitting the calculating of bud rate.In practical operation, the defect of the method is outside some
Full uniform health seed is seen, spits that bud situation is also fine, but the elongation of later stage plumular axis is undesirable, in production practice, this
The seed of sample can spit bud and show money or valuables one carries unintentionally under salt stress, but plumular axis does not extend and leads to not break ground.
Therefore lack a kind of method for screening salt tolerant cotton variety that planting environment is easy to control, easy to operate and high accuracy.
Invention content
To solve the above-mentioned problems, the present invention passes through salt treatment cotton plants selection and breeding salt tolerant cotton product using aerial fog cultivation
Kind.The planting environment of this method is easy to be uniformly controlled, cost is lower, detection cycle is shorter, the accuracy higher of salt tolerant detection simultaneously
It is easily operated to be convenient in each local promotion and implementation.
A method of screening salt tolerant cotton variety, it is characterised in that include the following steps：
Step 1：The culture solution of predetermined salinity is filled into container；
Step 2：The cotton seeds of different cultivars are immersed in culture solution；
Step 3：After cultivating the predetermined time, the cotton seeds normally to germinate are selected, the bud of cotton seeds is measured and record
It is long；
Step 4：The bud for comparing each kind cotton seeds of measurement is long.
A method of screening salt tolerant cotton variety includes the following steps：
The cotton seeds of different cultivars are respectively put into container by step S1, and the concentrated sulfuric acid is uniformly poured on seed and is stirred
It mixes until cotton seed short flannel purifies, plants shell nigrescence, clear water is then poured into container, and stir to cotton seeds and velveteen point
From, then it is clean with clear water elutriation；The uniform whole seed of size, plumpness is selected, respectively then with 70%
Alcoholic solution sterilization treatment 1min, washed 3 times after sterilizing or more；
Seed after washing is divided into salt treatment group and blank control group, salt treatment group and blank control group by step S2
It is respectively put into light culture 10~15 hours in the NaCl solution of distilled water and 150mmol/L, temperature is 28 DEG C ± 1 DEG C, relatively wet
Degree is 80% ± 2%；
Step S3, after culture the salt treatment group of each kind and blank control group choose respectively more growing points cracking,
The identical seed of bud length simultaneously records the long X1 of bud, and salt treatment group and blank control group are transplanted respectively to filling corresponding culture
It is cultivated in the aerial fog cultivation turnover box of liquid, the NaCl that 150mmol/L is filled in the corresponding aerial fog cultivation turnover box of salt treatment group is molten
Liquid fills distilled water in the corresponding aerial fog cultivation turnover box of blank control group, and aerial fog cultivation turnover box is controlled using time switch
In ultrasonic ultrasonic delay line memory often work 2~4 minutes, rest 3~7 minutes, often cross 20~24 hours replace culture solution；
Step S4, after cultivating 2~4 days, the bud for measuring each cotton seeds respectively is long, for the salt treatment group and sky of each kind
The cotton seeds of white control group calculate the long mean value of bud, and the long mean value of bud grows it to remove a maximum bud length and a most budlet
The long mean value of bud of average value afterwards, wherein salt treatment group is denoted as X2, and the long mean value of bud of space management group is denoted as X3；
Step S5 calculates amplitude of variation Y1, wherein Y1=X2- that each kind cotton seeds are grown by bud before and after salt treatment
X1,
Y1 values are bigger, show that the increased amplitude of the length of the cotton seeds bud after salt treatment is bigger, the cotton of corresponding kind
The salt tolerance of seed is better；
Step S6, if Y1 numerical value is identical, the cotton seeds salt treatment group of more same kind and the bud of blank control group
Long increment Y2, wherein Y2=X3-X2,
When Y2 values are positive value, numerical value is smaller, then shows salt treatment compared with blank control, and the bud length of the cotton variety declines
Amplitude it is smaller, illustrate that the salt tolerance of corresponding kind is better,
When Y2 values are negative value, the absolute value of the negative value is bigger, then shows salt treatment compared with blank control, the cotton variety
Bud grow that increased amplitude is bigger, illustrate that the salt tolerance of corresponding kind is better,
Y2 values correspond to the salt tolerance of kind when being negative value, got well when corresponding to Variety utilization when Y2 values are positive value.
Further, the aerial fog cultivation turnover box in the step S3 includes porous foam germination plate and babinet, is inserted per hole
Enter 1 cotton seeds, porous foam germination plate is placed in the babinet of turnover box.Further, the porous foam germination
Plate is expanded polystyrene foam germination plate.
Further, each kind of the cotton plants of salt treatment group and blank control group includes at least in the step S3
5 cotton seeds.
The cotton seeds of different cultivars are carried out concentrated sulfuric acid lint, so by the method for the screening salt tolerant cotton variety of the present invention
The uniform healthy seed of size, plumpness is selected after constant temperature drying afterwards, is divided into salt treatment group and sky after sterilizing
White control group carries out presoaking and germinating.The other factors that ensure that the germination percentage of cotton seeds and eliminate except kind tie screening
The influence of fruit.Soaking seed, a period of time, each kind chose more growing points crackings, the identical seed of buds length later, according to salt treatment
Group and blank control group, which are transplanted respectively into salt treatment aerial fog cultivation turnover box and blank control aerial fog cultivation turnover box, cultivates, and trains
It supports after terminating, the bud length of the germination cotton seeds of salt treatment group and blank control group is measured respectively, passes through cotton kind
The analysis comparison of the long data of bud of son can filter out the cotton variety more resistant to salt.This method compared with the prior art for, plant
Training environment is easy to be uniformly controlled, cost is lower, detection cycle is shorter, the accuracy higher of salt tolerant detection and it is easily operated convenient for
Each place promotion and implementation.
Description of the drawings
Fig. 1 is the method flow diagram of present invention screening salt tolerant cotton variety.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes.Based on the embodiments of the present invention, those of ordinary skill in the art institute without creative efforts
The every other embodiment obtained, shall fall within the protection scope of the present invention.
A method of screening salt tolerant cotton variety specifically includes following steps：
The selection and processing of seed：The cotton seeds of different cultivars are subjected to concentrated sulfuric acid lint respectively, concentrated sulfuric acid lint is
Cotton seeds are put into container, are uniformly poured on sulfuric acid on seed according to use ratio, side bevelling stirs de- to cotton seed short flannel
Only, until kind shell blacks, 10~15 minutes (seed moisture content is fewer, and the time is shorter) is about needed, clear water is then poured into container, and
Stirring is detached to cotton seeds with velveteen, then is eluriated totally with clear water.Size is picked out from the cotton seeds after lint, is satisfied
The uniform whole seed of full scale carries out a large amount of subtle villus that lint processing eliminates the surface of the seed to cotton seeds, has
Conducive to the selected of cotton seeds.Then cotton seeds after lint are put into sterilization treatment in 70% alcoholic solution again
1min or so is washed with distilled water for 3 times or more again after sterilizing.Sterilization treatment eliminates the most germ of the surface of the seed, disappears
In addition to the pest and disease damage hidden danger of seed germination phase, it ensure that the germination percentage of cotton seeds and eliminate the other factors pair except kind
As a result influence.
The grouping and vernalization of seed：Each kind cotton seeds after washing are all divided into salt treatment group and blank control
The each kind of group, the wherein cotton seeds of salt treatment group and blank control group includes at least 5, better assures that different plants
Influence of the strain individual to experimental result.Salt treatment group and blank control group are respectively put into distilled water and the NaCl of 150mmol/L is molten
It is cultivated 10~15 hours in liquid, condition of culture is：The phjytotron of 28 DEG C ± 1 DEG C of temperature, relative humidity 80% ± 2%, and
For light culture.Since cotton seeds are to steep progress vernalization processing in a liquid, indoor humidity is to germination percentage and speed of germination
Influence it is little, but the present invention or preferably relative humidity be 80% ± 2%.Simultaneously because cotton is thermophilic crops, cotton seed hair
The minimum temperature of bud is 10.5~12 DEG C, and preference temperature is 28~30 DEG C, and maximum temperature is 40~45 DEG C.In preference temperature scope
Interior, germination percentage is maintained at a higher level and germination is more quick.Table 1 shows that indoor relative humidity is 80% ± 2%
When different temperatures under cotton seeds germination percentage and speed of germination.
Table 1
As can be seen from the above table 28 DEG C when cotton seeds germination percentage relative to the significant raising of other temperature.Germination
The differentiation of seed is cultivated：Choose more lifes after vernalization culture respectively from the salt treatment group and blank control group of each kind
Long point cracking, the identical seed of bud length simultaneously record the long X1 of bud, then transplant respectively to the aerosol for filling corresponding culture solution
It cultivates in turnover box, the culture solution wherein filled in the corresponding aerial fog cultivation turnover box of salt treatment group is the NaCl of 150mmol/L
Solution, the culture solution filled in the corresponding aerial fog cultivation turnover box of blank control group are distilled water.The aerial fog cultivation has enough to meet the need luggage
Include the turnover box for filling corresponding culture solution and the porous foam being placed in turnover box germination plate, it is preferred to use the polystyrene in 40 holes
Foam germination plate, 1 cotton seeds is inserted into per hole.It is punched at a certain distance on polystyrene foam plate, self-control obtains polyphenyl
Vinyl foam germination plate.In the case where ensureing not damaging seed and its plumule, 1 cotton seeds is placed per hole, kind when placement
The hypocotyl of sub- plumule is inserted vertically into downward in foam plate hole, and ensures that hypocotyl reaches in the case where seed is not fallen
Cystosepiment bottom can be absorbed into the culture solution in aerial fog cultivation turnover box.The present invention implement when be preferably in each volume
The culture solution of 10L is poured into the aerial fog cultivation turnover box of 25L.Using timing when cotton seeds are cultivated in aerial fog cultivation turnover box
Atomizer service intermittent in switch control aerial fog cultivation turnover box, the plumule for generating culture solution aerosol for cotton seeds are inhaled
It receives, it is preferred to use ultrasonic ultrasonic delay line memory, the factors such as convenient and efficient and noiseless when using, vibrations of ultrasonic ultrasonic delay line memory influence, surpass
Then Sonic nebulizers, which work 2~4 minutes, rests 3~7 minutes alternately, by experimental verification work then rest 5 in 3 minutes
Minute is best discontinuous operation frequency, and the preferred working frequency of the ultrasonic ultrasonic delay line memory of the present invention, different operating frequency
Under influence of the ultrasonic ultrasonic delay line memory to experimental result it is as shown in table 2.
Table 2
In general, culture solution consumes slower, smaller to the loss of atomizer, plumule growth conditions and plumule to culture solution
The better scheme of absorptivity more meet the requirement of the present invention, wherein the scheme works that 3 minutes rest 5 minutes that work are best.Often
20~24 hours culture solutions replaced in turnover box are crossed, prevent culture solution volatilization or the concentration difference caused by absorbing excessive,
To keep, culture solution is fresh enough and the enough cotton germ growths of concentration.The volume of turnover box and the volume of culture solution can be with
It voluntarily selectes, portion of the present invention limits this, and the present invention is preferably one with the culture solution of 10L is added in the turnover box of 25L
Example.Wherein, select growing point cracking, the identical seed of bud length be consistent in order to control primary condition, be unlikely to interfere
Test result.Selection for the concentration of NaCl, inventor have found that NaCl is dense by carrying out research to common salinized soil
Degree is that the culture solution liquid of 150mmol/L more agrees with common salt-soda soil environment.It is excessively high, it has been more than salinized soil to cotton
Growth influence, general cotton cannot survive or shaky in excessively high salinized soil.It is too low then no good
Screening effect, the difference unobvious between salt treatment group and blank control group.Table 3 is containing for the various concentration of inventor's experiment
Influence of the culture solution of NaCl to cotton.Wherein, NaCl concentration is too low, does not have a due salt treatment effect, it is excessively high then significantly
The salt tolerant for having exceeded existing salt-tolerant plant is withstood forces without referential.
Table 3
Bud length measures：Cultivating 2~4 days after processing again, (preferably 3 days, the time, too short bud grew no significant change, and too long bud is long
Easily it is affected by other factors), the bud for measuring each cotton seeds respectively is long, the salt treatment group and blank control group for each kind
Cotton seeds calculate the long mean value of bud, since the salt treatment group and blank control group of the cotton seeds of each kind include at least 5
Grain is then the average value after removing a maximum bud length and a most budlet length in the long mean value of bud, further eliminates accidentally
The influence of situation and other factors.The long mean value of bud of wherein salt treatment group is denoted as X2, and the long mean value of bud of space management group is denoted as X3.
Salt tolerance preliminary comparison：The amplitude of variation Y1 that each kind cotton seeds are grown by bud before and after salt treatment is calculated, wherein
Y1=X2-X1, Y1 value are bigger, show that the increased amplitude of the length of the cotton seeds bud after salt treatment is bigger, the cotton of corresponding kind
The salt tolerance of flower seed is better；
Salt tolerance further compares：If Y1 numerical value is identical, the cotton seeds salt treatment group and blank of more same kind
The long increment Y2 of bud of control group, wherein Y2=X3-X2, when Y2 values are positive value, numerical value is smaller, then shows the same blank control of salt treatment
It compares, the amplitude that the bud length of the cotton variety declines is smaller, illustrates that the salt tolerance of corresponding kind is better；It, should when Y2 values are negative value
The absolute value of negative value is bigger, then shows salt treatment compared with blank control, and the increased amplitude of bud length of the cotton variety is bigger, says
The salt tolerance of bright corresponding kind is better；Y2 values correspond to the salt tolerance of kind when being negative value, more resistance to correspond to kind when positive value than Y2 value
Salt will be got well.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (4)
1. a kind of method of screening salt tolerant cotton variety, which is characterized in that include the following steps：
The cotton seeds of different cultivars are respectively put into container by step S1, the concentrated sulfuric acid are uniformly poured on seed and are stirred extremely
Cotton seed short flannel purifies, plants shell black until, clear water is then poured into container, and stir and detached to cotton seeds with velveteen, then
It is eluriated with clear water clean；The uniform whole seed of size, plumpness is selected, it is then molten with 70% alcohol respectively
Liquid sterilization treatment 1min, washed 3 times after sterilizing or more；
Seed after washing is divided into salt treatment group and blank control group, salt treatment group and blank control group difference by step S2
Light culture 10~15 hours in the NaCl solution of distilled water and 150mmol/L are put into, temperature is 28 DEG C ± 1 DEG C, relative humidity is
80% ± 2%；
Step S3, it is long to choose more growing point crackings, buds respectively for the salt treatment group of each kind and blank control group after culture
The identical seed of length simultaneously records the long X1 of bud, and salt treatment group and blank control group are transplanted respectively to filling corresponding culture solution
It is cultivated in aerial fog cultivation turnover box, the NaCl solution of 150mmol/L is filled in the corresponding aerial fog cultivation turnover box of salt treatment group, it is empty
Distilled water is filled in the corresponding aerial fog cultivation turnover box of white control group, is controlled using time switch super in aerial fog cultivation turnover box
Sonic nebulizers often work 2~4 minutes, rest 3~7 minutes, often spend 20~24 hours and replace culture solution；
Step S4, after cultivating 2~4 days, the bud for measuring each cotton seeds respectively is long, for the salt treatment group and blank pair of each kind
The long mean value of bud is calculated according to the cotton seeds of group, the long mean value of bud is after removing a maximum bud length and a most budlet length
The long mean value of bud of average value, wherein salt treatment group is denoted as X2, and the long mean value of bud of space management group is denoted as X3；
Step S5 calculates amplitude of variation Y1, wherein Y1=X2-X1 that each kind cotton seeds are grown by bud before and after salt treatment,
Y1 values are bigger, show that the increased amplitude of the length of the cotton seeds bud after salt treatment is bigger, the cotton seeds of corresponding kind
Salt tolerance it is better；
Step S6, if Y1 numerical value is identical, the cotton seeds salt treatment group of more same kind and the bud length of blank control group increase
Y2, wherein Y2=X3-X2 are measured,
When Y2 values are positive value, numerical value is smaller, then shows salt treatment compared with blank control, the width of the bud length decline of the cotton variety
It spends smaller, illustrates that the salt tolerance of corresponding kind is better,
When Y2 values are negative value, the absolute value of the negative value is bigger, then shows salt treatment compared with blank control, the bud of the cotton variety
Long increased amplitude is bigger, illustrates that the salt tolerance of corresponding kind is better,
Y2 values correspond to the salt tolerance of kind when being negative value, got well when corresponding to Variety utilization when Y2 values are positive value.
2. the method for screening salt tolerant cotton variety according to claim 1, which is characterized in that the aerosol in the step S3
Cultivation turnover box includes porous foam germination plate and babinet, and 1 cotton seeds is inserted into per hole, and porous foam germination plate is placed in week
In the babinet of turnning box.
3. the method for screening salt tolerant cotton variety according to claim 2, which is characterized in that the porous foam germination plate
For expanded polystyrene foam germination plate.
4. the method for screening salt tolerant cotton variety according to claim 1, which is characterized in that salt treatment in the step S3
The each kind of the cotton plants of group and blank control group includes at least 5 cotton seeds.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521606A (en) * | 2014-12-11 | 2015-04-22 | 南京农业大学 | Soybean salt tolerance and saline-alkaline tolerance screening device and identification method |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521606A (en) * | 2014-12-11 | 2015-04-22 | 南京农业大学 | Soybean salt tolerance and saline-alkaline tolerance screening device and identification method |
| CN104604555A (en) * | 2015-02-03 | 2015-05-13 | 中国农业科学院棉花研究所 | Method for screening saline-alkaline resistant/tolerant cotton |
| CN105210861A (en) * | 2015-10-29 | 2016-01-06 | 邯郸市农业科学院 | A kind of cotton seeds selection of salt tolerance method |
Non-Patent Citations (2)
| Title |
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| 新疆主要棉花品种耐盐性筛选与鉴定;杨淑萍 等;《干旱区研究》;20131130;第30卷(第6期);第1129-1134页 * |
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