CN106034463A - Method for promoting germination of oat aged seeds and growth of seedlings - Google Patents
Method for promoting germination of oat aged seeds and growth of seedlings Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention belongs to the technical field of seed vigor improvement and seed germination promotion, and discloses a method for promoting germination of oat aged seeds and growth of seedlings. The method comprises the following steps: firstly, determining the initial water content of the oat aged seeds to be tested, and after adjusting the water content, soaking the oat aged seeds for 24-36 hours by using a melatonin solution with the concentration of 1 (mu) M-1,000 (mu) M, then taking out the oat aged seeds to be tested and washing with distilled water; and finally, drying until the water content of the oat aged seeds to be tested is equal to the initial water content. In an existing seed priming technology, priming time and concentrate of a priming agent are affected by kinds of seeds and initial quality of the seeds and are not determined, specific parameters of the oat aged seeds primed by melatonin are determined by research, improvement and application of a technology of priming and treating the oat aged seeds by using the melatonin are realized, and the standard operation method for promoting germination of the oat aged seeds and improving vigor of the aged seeds is provided.
Description
Technical field
The invention belongs to improve seed vitality and promote seed germination technical field, be specifically related to one and utilize melatonin to promote
Enter Herba bromi japonici Aged Seeds to sprout and the method for growth of seedling.
Background technology
Herba bromi japonici (Avena sativa L.) is the annual forage plant of grass family Avena.High quality grass as poultry
Material, oat leaf and straw viridescent succulence, good palatability, crude protein, crude fat content are high, are of high nutritive value, and both can make blue or green dry
Grass, the most modulated ensilage.Meanwhile, Herba bromi japonici drought resisting, the feature of strong stress resistance determine its evil that can adapt to arid area
Bad environment, increases pastoral area and half farming and pastoral area vegetative coverage area, is beneficial to reduce rainwash, and soil protection fertility, to improving life
State environment has important effect.Additionally, keep healthy crop as green nourishing, containing a large amount of protein, solvable in oat seed
Property dietary fiber, vitamin, mineral etc., these nutrient substance are most important to human health.But, oils and fats in oat seed
Content higher (3.1%-11.6%), and linoleic acid content accounts for the 38.1%-52.0% of fatty acid, causes it in storage
It is easier to because fat derivant becomes sour aging, reduces the value of seed, and then to forage grass production, animal husbandry development and life
State environmental conservation adversely affects.
Melatonin (Melatonin, MT) is the indole derivatives of a kind of tryptophan.In animal body, the physiology of melatonin is made
With being furtherd investigate, there is the functions such as regulation biological clock, enhancing human body immunity ability, antioxidation.In recent years, melatonin
Being detected in increasing plant, the functional study of growth and development of plants and metabolism is the most increasingly closed by it by people
Note.Numerous studies show, in plant, melatonin has the physiological functions such as removing free radical, regulation photoperiod, protection chlorophyll.This
Outward, melatonin also plays important work in the abiotic stress such as plant resistant heavy metal and chemical contamination, ultraviolet and ionizing radiation
With.But, appear in the newspapers not yet about melatonin research in terms of improving Aged Seeds activity level, promotion Aged Seeds sprouting
Road.
Seed priming is that a kind of seed that controls slowly absorbs water and the preconditioning technique of progressively Hui Gan, and the principle of this technology is logical
Cross seed fully to absorb water to a certain extent, make cell membrane and organelle be fully repaired, promote Interior Seed substance metabolism, carry
High material converts and synthesis capability, it is ensured that it has certain presprouting metabolism, prevents again radicle from stretching out.Elicitation technique includes liquid
Body causes, solid matrix causes, biology causes, plant growth regulator causes and chemistry initiation.Seed priming techniques improves plants
The effect of sub-vigor is affected by many factors, and the initiation time is one of most important factor.If the time of initiation is the shortest, seed can not
Fully water suction, causes and is difficult to have effect；If the time of initiation is oversize, seed radicle in elicitation procedure breaks through seed coat, and then returns
Dry damage seed, causes seed vitality to decline.It addition, the concentration of initiator is also the key factor determining seed priming effect,
There are differences because seed or kind are different, the suitableeest initiation concentration need to be determined according to the effect reached required for causing.
So far, it is showed no both at home and abroad about using melatonin initiation to process the Aged Seeds vigor that improves, promote always
Change seed germination report, more lack science, system be applicable to a certain or initiation conditions of certain class seed.Therefore, probe into
Melatonin initiation processes the effect to Aged Seeds vigor, and screening determines the suitable initiation bar being applicable to a certain or certain class seed
Part, is the problem needing in actual agricultural production to solve.
Summary of the invention
It is an object of the invention to provide a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the side of growth of seedling
Method, the technical scheme taked is as follows:
A kind of melatonin promotion Herba bromi japonici Aged Seeds sprouting and method of growth of seedling of utilizing: first mensuration supplies examination Herba bromi japonici old
Changing the initial water content of seed, after water content adjusts, the melatonin solution soaking using concentration to be 1 μM-1000 μMs processes
24h-36h, then will take out for examination Herba bromi japonici Aged Seeds and after distilled water cleaning, Hui Gan will be to its initial water content.
The described germination percentage for examination Herba bromi japonici Aged Seeds is 52%-70%.
Use the adjustment to carrying out water content for examination Herba bromi japonici Aged Seeds of the saturated salt solution balance static weighing method.
The water content for examination Herba bromi japonici Aged Seeds after water content adjusts is 9%-11%.
Preferably, the water content for examination Herba bromi japonici Aged Seeds after water content adjusts is 10%.
The melatonin solution using concentration to be 1 μM is to for examination Herba bromi japonici Aged Seeds immersion treatment 36h.
The invention have the benefit that and the present invention is directed to existing Seed priming techniques causes time and initiator concentration
The uncertainty affected by Bamboo resource and seed initial mass (seed vitality level), by having determined melatonin initiation
The design parameter of Herba bromi japonici Aged Seeds, i.e. melatonin cause the time to be 36h, and melatonin concentration is 1 μM.At technology application program
On, define a set of by the standardization behaviour selected for examination Herba bromi japonici Aged Seeds, seed moisture content adjusts and melatonin initiation processes
Make system, it is to avoid due to laboratory or technical staff is different and the error that causes in technology application process；Achieve melatonin
Initiation processes improving and application of Herba bromi japonici Aged Seeds technology, for promoting Herba bromi japonici Aged Seeds to sprout and improving Aged Seeds vigor
Provide standardized operational approach.The technology of the present invention can be used for the aged seed of pretreatment Herba bromi japonici storage before field sowing, is beneficial to
Improve the Seedling emergence rate of Herba bromi japonici Aged Seeds, improve the upgrowth situation of seedling, it is to avoid cause economic loss to agricultural production.
Accompanying drawing explanation
Fig. 1 is that the initiation process of variable concentrations melatonin affects schematic diagram to Herba bromi japonici Aged Seeds germination percentage.
Fig. 2 is that the initiation process of variable concentrations melatonin affects schematic diagram to Herba bromi japonici Aged Seeds germination index.
Fig. 3 is that the initiation process of variable concentrations melatonin affects schematic diagram to Herba bromi japonici Aged Seeds vitality index.
Fig. 4 is that the initiation process of variable concentrations melatonin affects schematic diagram to Herba bromi japonici Aged Seeds seedling root length.
Fig. 5 is that the initiation of variable concentrations melatonin processes Herba bromi japonici Aged Seeds seedling root ghost image sound schematic diagram.
Fig. 6 is that the initiation process of variable concentrations melatonin affects schematic diagram to Herba bromi japonici Aged Seeds seedling Seedling length.
The initiation that Fig. 7 is variable concentrations melatonin processes and Herba bromi japonici Aged Seeds seedling Seedling ghost image is rung schematic diagram.
Fig. 8 is that the initiation of variable concentrations melatonin processes the impact signal of Herba bromi japonici Aged Seeds seedling vigor indexes Index
Figure.
Detailed description of the invention
With embodiment, the present invention is described further below in conjunction with the accompanying drawings, but protection scope of the present invention is not limited to this.
Embodiment 1
Select germination percentage to be respectively the Herba bromi japonici Aged Seeds of 52% and 70% as material to be tested, be denoted as respectively G52 and
G70, concrete germination is shown in Table 1.
Table 1: for examination Herba bromi japonici Aged Seeds germination
With reference to the regulation of international Seed Inspection association (ISTA, 2015) Seed Inspection code the 9th chapter, measure oat seed
Initial water content.According to the initial water content of oat seed, calculate weight corresponding when oat seed water content is 10%.
Use saturated salt solution balance static weighing method, oat seed is put into and fills saturated NaBr solution (equilibrium relative humidity (ERH) 57%)
Hermetic container inner equilibrium, weigh oat seed weight, till treating that oat seed reaches required weight.
Herba bromi japonici Aged Seeds with 0 μM, 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs of melatonin solution (be denoted as C,
M1, M10, M100, M200, M500, M1000), under 20 DEG C of dark conditions process 0h, 12h, 24h and 36h (be denoted as T0, T12,
T24, T36), change a solution every 12h, seed weight: liquor capacity (w/v)=1:3；After reaching the stipulated time, seed is taken
Going out, distilled water flushing 3 times, filter paper blots the surface of the seed moisture, 20 DEG C, 33%RH dark condition does next time to initial water content.
With reference to ISTA (2015) regulation, each process is respectively chosen uniform full 50 consistent, seed, is respectively put into
Filling in 11cm × 11cm Petri dish of three metafiltration paper, filter paper 10mL distilled water moistening, then in illumination box
(GXZ-380B-LED), in, cultivate under the conditions of 20 DEG C of illumination 8h, dark 16h, after germination in 10 days terminates, calculate 10d and send out
Bud terminate after germination percentage (G), result is as shown in table 2；Measure the root length (as shown in table 5) of seedling, root weight (such as table 6 institute simultaneously
Show), Seedling length (as shown in table 7) and Seedling weight (as shown in table 8), calculating germination index (GI), Seed Integrated vigor Index (VI) and seedling
Vitality index (SVI), result is respectively as shown in table 3, table 4 and table 9.
Germination percentage (G)=(G10/N) × 100%, wherein G10 is front 10d all normal seedling numbers, and N is for total for planting experimentally son
Number.
Germination index (GI)=∑ (Gt/Dt)；
Seed Integrated vigor Index (VI)=∑ (Gt/Dt) × WF；
Seedling vigor index (SVI)=(ARL+ASL) × G；
Wherein, Gt is the germinative number of t d, and Dt is germination natural law, and WF is the fresh weight (g) of seedling, and ARL is average root length
(cm), ASL is average Seedling length (cm).
Table 2: melatonin causes the time impact on Herba bromi japonici Aged Seeds germination percentage
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Table 3: melatonin causes the time impact on Herba bromi japonici Aged Seeds germination index
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Table 4: melatonin causes the time impact on Herba bromi japonici Aged Seeds vitality index
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Knowable to table 2, table 3 and table 4, compared with the control (MT causes the time to be 0h), the process of melatonin initiation 12h is notable
(P ＜ 0.05) reduces the average germination percentage of Herba bromi japonici Aged Seeds, mean germination index and average vitality index, and causes 24h
Process with 36h is then notable (P ＜ 0.05) improves the average germination percentage of seed, mean germination index and average vitality index,
And cause the result notable (P ＜ 0.05) of 36h higher than the result causing 24h.After melatonin causes 36h, G52 seed
Average germination percentage, mean germination index and average vitality index be respectively increased 22%, 2.88 and 9.71 compared with the control；
The average germination percentage of G70 seed, mean germination index and average vitality index have been respectively increased 10%, 0.87 and compared with the control
3.69.Visible, melatonin cause 36h process to the average germination percentage of G52 and G70 Herba bromi japonici Aged Seeds, mean germination index and
Average vitality index all increases significantly effect, but becomes apparent from the action effect of G52 seed.
Table 5: melatonin causes the time impact on Herba bromi japonici Aged Seeds seedling root length
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
As known from Table 5, Herba bromi japonici Aged Seeds causes the process of different time through melatonin, although causing 24h and 36h process
Average root length notable (P ＜ 0.05) higher than causing process of 12h, but the average root length causing 12h, 24h and 36h to process is the most aobvious
Write (P ＜ 0.05) less than comparison.The seedling root length of G52 Herba bromi japonici Aged Seeds is notable (P ＜ after melatonin causes 12h and 24h
0.05) less than comparison, and not notable (P ＞ 0.05) with contrast difference after causing 36h；The seedling root length of G70 Herba bromi japonici Aged Seeds
Then after melatonin causes 12h and 36h, notable (P ＜ 0.05) is less than comparison, and causes 24h result not show with contrast difference
Write (P ＞ 0.05).
Table 6: melatonin causes the time impact on Herba bromi japonici Aged Seeds seedling root weight
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
As known from Table 6, after the average seedling root of Herba bromi japonici Aged Seeds focuses on melatonin initiation 12h, notable (P ＜ 0.05) is low
In comparison, and cause 24h and 36h and contrast difference not notable (P ＞ 0.05).G52 Herba bromi japonici Aged Seeds causes not through melatonin
Same time-triggered protocol, compared with the control, causes the process notable (P ＜ 0.05) of 12h and 24h to reduce the root weight of oat seedlings, and
Cause process then notable (P ＜ 0.05) the root weight that improve oat seedlings of 36h；The seedling root weight warp of G70 Herba bromi japonici Aged Seeds
Melatonin causes different time to process, and compared with the control, when causing the process of 12h, notable (P ＜ 0.05) is less than comparison, causes
The process of 36h and contrast difference be not notable (P ＞ 0.05), causes the process notable (P ＜ 0.05) of 24h higher than comparison.
Table 7: melatonin causes the time impact on Herba bromi japonici Aged Seeds seedling Seedling length
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Table 8: melatonin causes the time impact on Herba bromi japonici Aged Seeds seedling Seedling weight
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Table 9: melatonin causes the time impact on Herba bromi japonici Aged Seeds seedling vigor index
Note: the meansigma methods that when data are the same initiation time in table, variable concentrations processes；Different lower cases represent same column
Significant difference (P ＜ 0.05) between meansigma methods.
Knowable to table 7,8 and 9, compared with the control, melatonin causes the process notable (P ＜ 0.05) of 12h to reduce seedling
Average Seedling length, average Seedling weight and average seedling vigor index；The process notable (P ＜ 0.05) causing 24h and 36h improves
The average Seedling weight of seedling and average seedling vigor index, and cause the process notable (P ＜ 0.05) of 36h higher than causing 24h process；
Average Seedling length only obtains notable (P ＜ 0.05) and improves when melatonin causes 24h, and and causes difference between the process of 36h not show
Write (P ＞ 0.05).But, affected by for examination oat seed sample germination percentage (activity level) is different, swallow after melatonin initiation
The maximum increase rate of wheat seedling average Seedling length, average Seedling weight and average seedling vigor index is the most different, and G52 seedling is respectively increased
0.38cm (T36), 0.60g (T24) and 306.59 (T36), G70 seedling has been respectively increased 0.86cm (T36), 0.22g (T24)
With 158.78 (T24).
In sum, for examination Herba bromi japonici Aged Seeds in melatonin causes the process of 24h and 36h, germination percentage, germination refer to
Number, Seed Integrated vigor Index and seedling vigor index are all significantly improved.
Embodiment 2
The Herba bromi japonici Aged Seeds selecting germination percentage to be 52% germination levels different with 70% two is as material to be tested, respectively
It is denoted as G52 and G70.
With reference to the regulation of international Seed Inspection association (ISTA, 2015) Seed Inspection code the 9th chapter, measure oat seed
Initial water content.According to the initial water content of oat seed, calculate weight corresponding when oat seed water content is 10%.
Use saturated salt solution balance static weighing method, oat seed is put into and fills saturated NaBr solution (equilibrium relative humidity (ERH) 57%)
Hermetic container inner equilibrium, weigh oat seed weight, till treating that oat seed reaches required weight.
Oat seed is respectively with the melatonin solution of 0 μM, 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs concentration
(being denoted as C, M1, M10, M100, M200, M500, M1000), causes 12h, 24h and 36h (to be denoted as under 20 DEG C of dark conditions respectively
T12, T24, T36), change a solution every 12h, seed weight: liquor capacity (w/v)=1:3；After reaching the stipulated time, will plant
Son takes out, distilled water flushing 3 times, and filter paper blots the surface of the seed moisture, 20 DEG C, 33%RH dark condition does next time to initially containing
The water yield.
With reference to ISTA (2015) regulation, each process is respectively chosen uniform full 50 consistent, seed, is respectively put into
Filling in 11cm × 11cm Petri dish of three metafiltration paper, filter paper 10mL distilled water moistening, then in illumination box
(GXZ-380B-LED), in, cultivate under the conditions of 20 DEG C of illumination 8h, dark 16h, after germination in 10 days terminates, calculate 10d and send out
Bud terminate after germination percentage (G), result is as shown in Figure 1；Measure the root length (as shown in Figure 4) of seedling, root weight (such as Fig. 5 institute simultaneously
Show), Seedling length (as shown in Figure 6) and Seedling weight (as shown in Figure 7), calculate germination index (GI), Seed Integrated vigor Index (VI) and seedling
Vitality index (SVI), result is respectively as shown in Fig. 2, Fig. 3 and Fig. 8.
From fig. 1, it can be seen that G52 and G70 Herba bromi japonici Aged Seeds is after the melatonin initiation of variable concentrations processes, and to photograph
Ratio, when melatonin causes 24h, the melatonin of 100 μMs, 200 μMs and 500 μMs concentration all notable (P ＜ 0.05) can improve G52 Herba bromi japonici
The germination percentage of Aged Seeds, and only the melatonin of 200 μMs of concentration significantly (P ＜ 0.05) can improve G70 Herba bromi japonici Aged Seeds
Germination percentage；When melatonin causes 36h, the melatonin of 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs concentration all can show
Write (P ＜ 0.05) and improve the germination percentage of G52 and G70 Herba bromi japonici Aged Seeds.
As can be seen from Figure 2, when melatonin causes 24h, the melatonin of 100 μMs and 200 μMs concentration all can notable (P ＜ 0.05) carry
The germination index of high G52 and G70 Herba bromi japonici Aged Seeds；When causing 36h, 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs
The melatonin of concentration all notable (P ＜ 0.05) can improve the germination index of G52 Herba bromi japonici Aged Seeds, and only 1 μM, 10 μMs and
The melatonin of 100 μMs of concentration can significantly (P ＜ 0.05) improve G70 Herba bromi japonici Aged Seeds germination index.
As can be seen from Figure 3, G52 and G70 Herba bromi japonici Aged Seeds is after the melatonin initiation of variable concentrations processes, and to photograph
Ratio, when melatonin causes 24h, the melatonin of 100 μMs and 200 μMs concentration all significantly (P ＜ 0.05) can improve G52 and G70 swallow
The vitality index of wheat Aged Seeds；When melatonin causes 36h, 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs of concentration
Melatonin all significantly (P ＜ 0.05) vitality index of G52 Herba bromi japonici Aged Seeds, and only 1 μM and 10 μMs of concentration can be improved
Melatonin notable (P ＜ 0.05) vitality index of G70 Herba bromi japonici Aged Seeds can be improved.
As can be seen from Figure 4, compared with the control, melatonin cause 12h, 24h and 36h time, 1 μM, 10 μMs, 100 μMs, 200 μMs,
Processing the seedling root length of G52 and G70 Herba bromi japonici Aged Seeds all significantly (P ＞ of 500 μMs and 1000 μMs each concentration
0.05) effect is improved.
As can be seen from Figure 5, compared with the control, G52 Herba bromi japonici Aged Seeds only causes 36h at melatonin, and concentration is 1 μM, 10 μ
M and when 100 μMs seedling root recuperation improve to notable (P ＜ 0.05)；And the process of each concentration all can not show during melatonin initiation 24h
Write (P ＞ 0.05) and improve its seedling root weight.G70 Herba bromi japonici Aged Seeds the most only melatonin cause 24h time seedling root recuperation to show
Write (P ＜ 0.05) to improve, and initiation concentration is 100 μMs and 200 μMs；And each concentration processes the most not when melatonin causes 36h
Notable (P ＞ 0.05) its seedling root weight can be improved.
As can be seen from Figure 6, compared with the control, G52 Herba bromi japonici Aged Seeds only melatonin cause 36h time seedling Seedling length shown
Write (P ＜ 0.05) to improve, and initiation concentration is 1 μM and 500 μMs；And during melatonin initiation 24h, each concentration processes and all can not show
The seedling Seedling writing (P ＞ 0.05) raising G70 Herba bromi japonici Aged Seeds is long.G70 Herba bromi japonici Aged Seeds melatonin cause 24h time, 1 μ
M, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs of each concentration all energy notable (P ＜ 0.05) raising seedling Seedlings are long；And melatonin
Cause 36h time, only the concentration of 1 μM can significantly (P ＜ 0.05) raising seedling Seedling long.
As can be seen from Figure 7, compared with the control, when melatonin causes 24h, 100 μMs and 200 μMs of concentration notable (P ＜ of all energy
0.05) the seedling Seedling weight of G52 and G70 Herba bromi japonici Aged Seeds is improved；During additionally, melatonin causes 36h, kind aging to G52 Herba bromi japonici
For son, 1 μM, 10 μMs, 100 μMs, 200 μMs, 500 μMs and 1000 μMs of each concentration all significantly (P ＜ 0.05) can improve its seedling
Miao Chong, and only 1 μM and 10 μMs of concentration significantly (P ＜ 0.05) can improve the seedling Seedling weight of G70 Herba bromi japonici Aged Seeds.
As it can be observed in the picture that compared with the control, G52 Herba bromi japonici Aged Seeds when melatonin causes 24h, only 100 μMs of concentration
Melatonin significantly (P ＜ 0.05) can improve its seedling vigor index, and when melatonin causes 36h, 1 μM, 10 μMs, 100 μMs,
The melatonin of 200 μMs, 500 μMs and 1000 μMs concentration all has significantly (P ＜ 0.05) raising effect to its seedling vigor index.G70
Herba bromi japonici Aged Seeds only melatonin cause 24h time seedling vigor index be significantly improved (P ＜ 0.05), and cause concentration be
1 μM, 100 μMs, 200 μMs, 500 μMs and 1000 μMs；And during melatonin initiation 36h, each concentration processes all can not significantly (P ＞
0.05) the seedling vigor index of G70 Herba bromi japonici Aged Seeds is improved.
In sum, the initiation of melatonin variable concentrations processes Herba bromi japonici Aged Seeds germination percentage, germination index, seed vitality
Index, root length, root weight, Seedling length, Seedling weight are different with the action effect of seedling vigor index, cause time length to have with melatonin
Close, affected by seed vitality level simultaneously.Table 10 reaches remarkable result for melatonin initiation to Herba bromi japonici Aged Seeds indices
Cause the time with cause concentration combination.
Table 10: melatonin causes the initiation time that Herba bromi japonici Aged Seeds indices reaches remarkable result and causes concentration
Combination
As known from Table 10, the melatonin of 1 μM of-1000 μMs of concentration is under 24h or the 36h initiation time, G52 or G70 Herba bromi japonici is old
Changing seed indices and all have action effect, but comprehensive two groups of results of G52 and G70 Herba bromi japonici Aged Seeds show, 1 μM of melatonin draws
Sending out 36h is the suitable initiation conditions processing G52 and G70 Herba bromi japonici Aged Seeds.
The two factor variance statistical results that melatonin initiation processes Herba bromi japonici Aged Seeds are as shown in table 11.
Table 11: melatonin initiation processes two factor variance statistical results of Herba bromi japonici Aged Seeds
As known from Table 11, melatonin causes time, melatonin to cause the time and that causes concentration makees mutually to G52 and G70 swallow
Wheat Aged Seeds germination percentage, germination index, Seed Integrated vigor Index, root length, root weight, Seedling length, Seedling weight and the shadow of seedling vigor index
Ring and all reach pole significant level (P ＜ 0.01).For G52 Herba bromi japonici Aged Seeds, melatonin cause concentration except to seedling root weight,
The impact of Miao Chong and seedling vigor index notable (P ＞ 0.05) outward, to percentage of seedgermination, germination index, Seed Integrated vigor Index,
Root length and Seedling length are respectively provided with significantly (P ＜ 0.05) impact, and to percentage of seedgermination, germination index, Seed Integrated vigor Index and seedling
The impact of root length reaches pole significant level (P ＜ 0.01).For G70 Herba bromi japonici Aged Seeds, melatonin causes concentration to seed
The impact of germination percentage, vitality index and seedling vigor index is not notable (P ＞ 0.05), and to seed germination index, root length, root
Weight, Seedling length and Seedling weight average have significantly (P ＜ 0.05) impact, and the impact on root length, root weight and Seedling length reaches pole significant level
(P ＜ 0.01).Visible, during melatonin causes Herba bromi japonici Aged Seeds, melatonin causes the time to be to affect the aging kind of Herba bromi japonici
Son is sprouted and the key factor of growth of seedling.In the melatonin set by this test causes time range, the long period causes
May advantageously facilitate Herba bromi japonici Aged Seeds to sprout and growth of seedling.Therefore, the different germination of G52 with G70 two kinds is processed according to melatonin
The initiation time of horizontal Herba bromi japonici Aged Seeds and initiation concentration results, determine that germination level is old the Herba bromi japonici of 52%-70% scope
The suitable condition changing seed employing melatonin initiation process is initiation time 36h, causes concentration 1 μM.
Claims (6)
1. one kind utilizes melatonin to promote the sprouting of Herba bromi japonici Aged Seeds and the method for growth of seedling, it is characterised in that described method
For: first measuring the initial water content for examination Herba bromi japonici Aged Seeds, after water content adjusts, using concentration is 1 μM-1000 μMs
Melatonin solution soaking processes 24h-36h, then will take out for examination Herba bromi japonici Aged Seeds and distilled water cleans, last Hui Ganzhi
Initial water content for examination Herba bromi japonici Aged Seeds.
The most according to claim 1 a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the method for growth of seedling,
It is characterized in that, the described germination percentage for examination Herba bromi japonici Aged Seeds is 52%-70%.
The most according to claim 1 a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the method for growth of seedling,
It is characterized in that, use saturated salt solution balance static weighing method to carry out water content adjustment to for examination Herba bromi japonici Aged Seeds.
The most according to claim 1 a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the method for growth of seedling,
It is characterized in that, the water content for examination Herba bromi japonici Aged Seeds after water content adjusts is 9%-11%.
The most according to claim 4 a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the method for growth of seedling,
It is characterized in that, the water content for examination Herba bromi japonici Aged Seeds after water content adjusts is 10%.
The most according to claim 1 a kind of utilize melatonin promote Herba bromi japonici Aged Seeds sprout and the method for growth of seedling,
It is characterized in that, the melatonin solution using concentration to be 1 μM is to for examination Herba bromi japonici Aged Seeds immersion treatment 36h.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107041181A (en) * | 2017-04-14 | 2017-08-15 | 四川农业大学 | It is a kind of to promote pea seedling growth, the method for reduction Cd accumulation |
| CN107079631A (en) * | 2017-06-21 | 2017-08-22 | 四川农业大学 | A kind of method for significantly reducing broad bean Cd accumulation and promoting it to grow |
| CN109104932A (en) * | 2018-08-11 | 2019-01-01 | 山东省潍坊市农业科学院 | A kind of pre-sowing treatment method improving asparagus Aged Seeds vigor |
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| CN111903844A (en) * | 2020-08-07 | 2020-11-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN112385352A (en) * | 2020-11-23 | 2021-02-23 | 河北农业大学 | Method for enhancing drought resistance of cotton seeds by melatonin and application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104718840A (en) * | 2015-02-13 | 2015-06-24 | 中国农业大学 | Method for promoting plant seed germination under cadmium stress |
-
2016
- 2016-05-26 CN CN201610363270.8A patent/CN106034463B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104718840A (en) * | 2015-02-13 | 2015-06-24 | 中国农业大学 | Method for promoting plant seed germination under cadmium stress |
Non-Patent Citations (1)
| Title |
|---|
| 赵燕等: "植物中褪黑素的研究进行", 《细胞植物学报》 * |
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| CN109104932A (en) * | 2018-08-11 | 2019-01-01 | 山东省潍坊市农业科学院 | A kind of pre-sowing treatment method improving asparagus Aged Seeds vigor |
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| CN111903844A (en) * | 2020-08-07 | 2020-11-10 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN111903844B (en) * | 2020-08-07 | 2022-05-13 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN112385352A (en) * | 2020-11-23 | 2021-02-23 | 河北农业大学 | Method for enhancing drought resistance of cotton seeds by melatonin and application |
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| CN115152771A (en) * | 2022-05-13 | 2022-10-11 | 中国科学院东北地理与农业生态研究所 | Application of melatonin in cultivation of suaeda glauca |
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