CN106034463B - A method of promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin - Google Patents
A method of promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
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Abstract
The invention discloses belong to a kind of method for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin for improving seed vitality and promoting seed Germination Technique field.The method is：The initial water content for trying oat Aged Seeds is measured first, after water content adjusts, use concentration for 1 μM -1000 μM of epiphysin solution immersion treatment -36h for 24 hours, then will for examination oat Aged Seeds take out and wash with distilled water, last Hui Gan to for examination oat Aged Seeds initial water content.The present invention is for the uncertainty that initiation time and initiator concentration are influenced by Bamboo resource and seed initial mass in existing Seed priming techniques, cause the design parameter of oat Aged Seeds by having determined epiphysin, it realizes epiphysin and causes improving and applying for processing oat Aged Seeds technology, provide standardized operating method to promote oat Aged Seeds to sprout and improving Aged Seeds vigor.
Description
Technical field
The invention belongs to improve seed vitality and promote seed Germination Technique field, and in particular to a kind of to be promoted using epiphysin
Into the sprouting of oat Aged Seeds and the method for growth of seedling.
Background technique
Oat (Avena sativa L.) is the annual forage plant of grass family Avena.High quality grass as livestock and poultry
Material, oat leaf and stalk viridescent succulence, palatability is good, and crude protein, crude fat content are high, and nutritive value is high, can both make green dry
Grass, and modulated ensilage.Meanwhile the strong feature of oat drought resisting, resistance determines that it can adapt to the evil of arid area
Bad environment increases pastoral area and half farming and pastoral area vegetative coverage area, is conducive to reduce rainwash, soil protection fertility is given birth to improving
State environment plays an important role.In addition, as green nourishing health care crop, a large amount of protein, solvable are contained in oat seed
Property dietary fiber, vitamin, minerals etc., these nutriments are most important to human health.However, grease in oat seed
Content is higher (3.1%-11.6%), and linoleic acid content accounts for the 38.1%-52.0% of fatty acid, causes it in storage
More easily aging due to fat derivant is rancid reduces the utility value of seed, and then to forage grass production, animal husbandry development and life
State environmental protection adversely affects.
Epiphysin (Melatonin, MT) is a kind of indole derivatives of tryptophan.The physiology of epiphysin is made in animal body
With having been furtherd investigate, have the function of to adjust biological clock, enhancing body immunity, anti-oxidant etc..In recent years, epiphysin
It is detected in more and more plants, the functional study of plant growth and development and metabolism is also increasingly closed by people
Note.A large number of studies show that epiphysin has removing free radical, adjusts the physiological functions such as photoperiod, protection chlorophyll in plant.This
Outside, epiphysin also plays important work in the abiotic stress such as plant resistant heavy metal and chemical contamination, ultraviolet and ionising radiation
With.However, the research in relation to epiphysin in terms of improving Aged Seeds activity level, promoting Aged Seeds sprouting does not appear in the newspapers yet
Road.
Seed priming is a kind of preconditioning technique that control seed slowly absorbs water with gradually Hui Gan, and the principle of the technology is logical
It crosses seed sufficiently to absorb water to a certain extent, cell membrane and organelle is made to be fully repaired, promote Interior Seed metabolism, mention
High substance conversion and synthesis capability, guarantee that it has certain presprouting metabolism, and prevent radicle from stretching out.Elicitation technique includes liquid
Body causes, solid matrix causes, biology causes, plant growth regulator causes and chemistry initiation.Seed priming techniques improve kind
The effect of sub- vigor is affected by many factors, and causing the time is one of most important factor.If causing, the time is too short, and seed cannot
Sufficiently water suction, initiation are difficult to have effect；If causing, the time is too long, and seed radicle in elicitation procedure is prominent breaks in the seed coat, and then returns
Dry damage seed, causes seed vitality to decline.In addition, the concentration of initiator is also to determine the key factor of seed priming effect,
It is had differences because seed kind or kind are different, need to determine most suitable initiation concentration according to effect to be achieved needed for causing.
So far, it is showed no about handling using epiphysin initiation the Aged Seeds vigor that improves both at home and abroad, promotes always
Change the report that seed is sprouted, more lacks science, system suitable for a certain or certain class seed initiation conditions.Therefore, it probes into
Epiphysin causes effect of the processing to Aged Seeds vigor, and screening, which determines, is suitable for a certain or certain class seed suitable initiation item
Part is problem to be solved in practical agricultural production.
Summary of the invention
The purpose of the present invention is to provide a kind of sides for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin
Method, the technical solution taken are as follows：
A method of promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin：Measurement is old for examination oat first
The initial water content for changing seed uses concentration for 1 μM -1000 μM of epiphysin solution immersion treatment after water content adjusts
- 36h for 24 hours, then will for examination oat Aged Seeds take out and wash with distilled water after, Hui Gan to its initial water content.
The germination percentage for trying oat Aged Seeds is 52%-70%.
Adjustment using saturated salt solution balance static weighing method to water content is carried out for examination oat Aged Seeds.
It is 9%-11% through the water content water content adjusted for trying oat Aged Seeds.
It preferably, is 10% through the water content water content adjusted for trying oat Aged Seeds.
Using concentration is 1 μM of epiphysin solution to for trying oat Aged Seeds immersion treatment 36h.
Beneficial effects of the present invention are：The present invention is for initiation time and initiator concentration in existing Seed priming techniques
By the uncertainty that Bamboo resource and seed initial mass (seed vitality is horizontal) influence, by having determined epiphysin initiation
The design parameter of oat Aged Seeds, i.e. epiphysin cause the time as 36h, and epiphysin concentration is 1 μM.In technical application program
On, it forms a set of standardization by causing processing for the selection of examination oat Aged Seeds, seed moisture content adjustment and epiphysin and grasps
Make system, avoid during technical application as laboratory or technical staff it is different and caused by error；Realize epiphysin
Cause improving and applying for processing oat Aged Seeds technology, to promote oat Aged Seeds to sprout and improving Aged Seeds vigor
Provide standardized operating method.The technology of the present invention can be used for the aged seed of pretreatment oat storage before the sowing of field, be conducive to
The Seedling emergence rate for improving oat Aged Seeds, improves the upgrowth situation of seedling, avoids causing economic loss to agricultural production.
Detailed description of the invention
Fig. 1 is that the initiation processing of various concentration epiphysin influences schematic diagram to oat Aged Seeds germination percentage.
Fig. 2 is that the initiation processing of various concentration epiphysin influences schematic diagram to oat Aged Seeds germination index.
Fig. 3 is that the initiation processing of various concentration epiphysin influences schematic diagram to oat Aged Seeds vitality index.
Fig. 4 is that the initiation processing of various concentration epiphysin influences schematic diagram to oat Aged Seeds seedling root long.
Fig. 5 is that the initiation processing of various concentration epiphysin rings schematic diagram to oat Aged Seeds seedling root ghost image.
Fig. 6, which is that the initiation processing of various concentration epiphysin is long on oat Aged Seeds seedling seedling, influences schematic diagram.
Fig. 7 is that the initiation processing of various concentration epiphysin rings schematic diagram to oat Aged Seeds seedling seedling ghost image.
Fig. 8 is that the initiation processing of various concentration epiphysin influences to illustrate on oat Aged Seeds seedling vigor indexes Index
Figure.
Specific embodiment
The present invention is described further with reference to the accompanying drawings and examples, but the scope of the present invention is not limited thereto.
Embodiment 1
Select germination percentage be respectively 52% and 70% oat Aged Seeds as material to be tested, be denoted as respectively G52 with
G70, specific germination are shown in Table 1.
Table 1：For trying oat Aged Seeds germination
Referring to the regulation of the 9th chapter of international Seed Inspection association (ISTA, 2015) Seed Inspection regulation, oat seed is measured
Initial water content.According to the initial water content of oat seed, weight corresponding when oat seed water content is 10% is calculated.
Static weighing method is balanced using saturated salt solution, oat seed is put into and fills saturation NaBr solution (equilibrium relative humidity (ERH) 57%)
Closed container inner equilibrium, the weight of oat seed is weighed, until reaching required weight to oat seed.
Oat Aged Seeds with 0 μM, 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM epiphysin solution (be denoted as C,
M1, M10, M100, M200, M500, M1000), handle under 20 DEG C of dark conditions 0h, 12h, for 24 hours with 36h (be denoted as T0, T12,
T24, T36), a solution, seed weight are changed every 12h：Liquor capacity (w/v)=1：3；After reaching the stipulated time, seed is taken
Out, distilled water flushing 3 times, filter paper blot the surface of the seed moisture, and 20 DEG C, 33%RH dark condition done next time to initial water content.
It is provided referring to ISTA (2015), each processing is each to choose uniformly full 50, consistent seed, is respectively put into
In the 11cm × 11cm Petri dish for filling three layers of filter paper, filter paper is soaked with 10mL distilled water, then in illumination box
(GXZ-380B-LED) it in, is cultivated under the conditions of 20 DEG C of illumination 8h, dark 16h, after germination in 10 days, calculating 10d hair
Germination percentage (G) after bud, the results are shown in Table 2；Root long (as shown in table 5), the root for measuring seedling simultaneously are heavy (such as 6 institute of table
Show), seedling long (as shown in table 7) and seedling weigh (as shown in table 8), calculating germination index (GI), Seed Integrated vigor Index (VI) and seedling
Vitality index (SVI), as a result respectively as shown in table 3, table 4 and table 9.
Germination percentage (G)=(G10/N) × 100%, wherein G10 is preceding 10d all normal seedling numbers, and N is sub total for planting experimentally
Number.
Germination index (GI)=∑ (Gt/Dt)；
Seed Integrated vigor Index (VI)=∑ (Gt/Dt) × WF；
Seedling vigor index (SVI)=(ARL+ASL) × G；
Wherein, Gt is the germinative number of t d, and Dt is germination number of days, and WF is the fresh weight (g) of seedling, and ARL is average root long
(cm), ASL is that average seedling is long (cm).
Table 2：Epiphysin causes influence of the time to oat Aged Seeds germination percentage
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
Table 3：Epiphysin causes influence of the time to oat Aged Seeds germination index
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
Table 4：Epiphysin causes influence of the time to oat Aged Seeds vitality index
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
From table 2, table 3 and table 4 it is found that (MT causes the time as 0h), and the processing that epiphysin causes 12h is significant compared with the control
(P < 0.05) reduces average germination percentage, mean germination index and the average vitality index of oat Aged Seeds, and causes for 24 hours
Processing with 36h then significant (P < 0.05) improves average germination percentage, mean germination index and the average vitality index of seed,
And the processing result significant (P < 0.05) for causing 36h is higher than the processing result caused for 24 hours.After epiphysin causes 36h, G52 seed
Average germination percentage, mean germination index and average vitality index 22%, 2.88 and 9.71 have been respectively increased compared with the control；
10%, 0.87 and has been respectively increased in average germination percentage, mean germination index and the average vitality index of G70 seed compared with the control
3.69.As it can be seen that epiphysin cause 36h processing to the average germination percentage of G52 and G70 oat Aged Seeds, mean germination index and
Average vitality index increases significantly effect, but becomes apparent to the function and effect of G52 seed.
Table 5：Epiphysin causes influence of the time to oat Aged Seeds seedling root long
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
As known from Table 5, oat Aged Seeds cause the processing of different time through epiphysin, handle for 24 hours with 36h although causing
Average root long significant (P < 0.05) be higher than the processing for causing 12h, but cause 12h, aobvious with the average root long of 36h processing for 24 hours
(P < 0.05) is write lower than control.The seedling root long of G52 oat Aged Seeds causes 12h and significant (P < rear for 24 hours in epiphysin
0.05) lower than control, and cause not significant (P > 0.05) with contrast difference after 36h；The seedling root long of G70 oat Aged Seeds
Then significant (P < 0.05) is lower than control after epiphysin causes 12h and 36h, and causes processing result for 24 hours and do not show with contrast difference
It writes (P > 0.05).
Table 6：Epiphysin causes influence of the time to oat Aged Seeds seedling root weight
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
As known from Table 6, the average seedling root of oat Aged Seeds focuses on low after epiphysin causes 12h significant (P < 0.05)
In control, and cause not significant (P > 0.05) with 36h and contrast difference for 24 hours.G52 oat Aged Seeds cause not through epiphysin
Same time-triggered protocol, compared with the control, causing 12h and processing for 24 hours significantly (P < 0.05) reduces the root weight of oat seedlings, and
Cause the processing of 36h and then significant (P < 0.05) improves the root weights of oat seedlings；The seedling root of G70 oat Aged Seeds passes through again
Epiphysin causes different time processing, and compared with the control, significant (P < 0.05) causes lower than control when causing the processing of 12h
The processing of 36h and contrast difference be not significant (P > 0.05), and the processing significant (P < 0.05) caused for 24 hours is higher than control.
Table 7：Epiphysin causes the time influence long to oat Aged Seeds seedling seedling
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
Table 8：Epiphysin causes influence of the time to oat Aged Seeds seedling seedling weight
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
Table 9：Epiphysin causes influence of the time to oat Aged Seeds seedling vigor index
Note：The average value of various concentration processing when data are the same initiation time in table；Different lowercases indicate same column
Significant difference (P < 0.05) between average value.
From table 7,8 and 9 it is found that compared with the control, the processing significant (P < 0.05) that epiphysin causes 12h reduces seedling
Average seedling is long, average seedling weight and average seedling vigor index；Cause and is improved for 24 hours with the processing of 36h significant (P < 0.05)
The heavy and average seedling vigor index of the average seedling of seedling, and the processing significant (P < 0.05) for causing 36h is higher than initiation and handles for 24 hours；
Averagely seedling is long only obtains significant (P < 0.05) raising when epiphysin causes for 24 hours, and difference is not shown between the processing of initiation 36h
It writes (P > 0.05).However, by for examination, oat seed sample germination percentage (activity level) is different is influenced, swallow after epiphysin causes
Wheat seedling is averaged, and seedling is long, average seedling is heavy and the maximum increase rate of averagely seedling vigor index is also different, and G52 seedling is respectively increased
0.86cm (T36), 0.22g (T24) has been respectively increased in 0.38cm (T36), 0.60g (T24) and 306.59 (T36), G70 seedling
With 158.78 (T24).
In conclusion causing in the processing with 36h for 24 hours for examination oat Aged Seeds in epiphysin, germination percentage, germination refer to
Number, Seed Integrated vigor Index and seedling vigor index are significantly improved.
Embodiment 2
Selecting germination percentage is the horizontal oat Aged Seeds of 52% and 70% two different germination as material to be tested, respectively
It is denoted as G52 and G70.
Referring to the regulation of the 9th chapter of international Seed Inspection association (ISTA, 2015) Seed Inspection regulation, oat seed is measured
Initial water content.According to the initial water content of oat seed, weight corresponding when oat seed water content is 10% is calculated.
Static weighing method is balanced using saturated salt solution, oat seed is put into and fills saturation NaBr solution (equilibrium relative humidity (ERH) 57%)
Closed container inner equilibrium, the weight of oat seed is weighed, until reaching required weight to oat seed.
Oat seed uses the epiphysin solution of 0 μM, 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM concentration respectively
(being denoted as C, M1, M10, M100, M200, M500, M1000) is caused 12h under 20 DEG C of dark conditions respectively, (is denoted as with 36h for 24 hours
T12, T24, T36), a solution, seed weight are changed every 12h：Liquor capacity (w/v)=1：3；After reaching the stipulated time, it will plant
Son takes out, and distilled water flushing 3 times, filter paper blots the surface of the seed moisture, and 20 DEG C, 33%RH dark condition done next time to initially containing
Water.
It is provided referring to ISTA (2015), each processing is each to choose uniformly full 50, consistent seed, is respectively put into
In the 11cm × 11cm Petri dish for filling three layers of filter paper, filter paper is soaked with 10mL distilled water, then in illumination box
(GXZ-380B-LED) it in, is cultivated under the conditions of 20 DEG C of illumination 8h, dark 16h, after germination in 10 days, calculating 10d hair
Germination percentage (G) after bud, as a result as shown in Figure 1；Root long (as shown in Figure 4), the root for measuring seedling simultaneously are heavy (such as Fig. 5 institute
Show), seedling long (as shown in Figure 6) and seedling weigh (as shown in Figure 7), calculating germination index (GI), Seed Integrated vigor Index (VI) and seedling
Vitality index (SVI), as a result respectively as shown in Fig. 2, Fig. 3 and Fig. 8.
From fig. 1, it can be seen that G52 and G70 oat Aged Seeds are after the epiphysin initiation of various concentration processing, and to photograph
Than when epiphysin is caused for 24 hours, the epiphysin of 100 μM, 200 μM and 500 μM concentration can significant (P < 0.05) raising G52 oat
The germination percentage of Aged Seeds, and the epiphysin of only 200 μM concentration being capable of significant (P < 0.05) raising G70 oat Aged Seeds
Germination percentage；When epiphysin causes 36h, the epiphysin of 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM concentration can be shown
Write the germination percentage that (P < 0.05) improves G52 and G70 oat Aged Seeds.
As can be seen from Figure 2, when epiphysin is caused for 24 hours, the epiphysin of 100 μM and 200 μM concentration can significantly (P < 0.05) be mentioned
The germination index of high G52 and G70 oat Aged Seeds；When causing 36h, 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM
The epiphysin of concentration can significant (P < 0.05) improve the germination index of G52 oat Aged Seeds, and only 1 μM, 10 μM and
The epiphysin of 100 μM of concentration is capable of the germination index of significant (P < 0.05) raising G70 oat Aged Seeds.
As can be seen from Figure 3, G52 and G70 oat Aged Seeds are after the epiphysin initiation of various concentration processing, and to photograph
Than when epiphysin is caused for 24 hours, the epiphysin of 100 μM and 200 μM concentration being capable of significant (P < 0.05) raising G52 and G70 swallow
The vitality index of wheat Aged Seeds；When epiphysin causes 36h, 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM concentration
Epiphysin be capable of the vitality index that significant (P < 0.05) improves G52 oat Aged Seeds, and only 1 μM and 10 μM of concentration
Epiphysin can significant (P < 0.05) improve the vitality index of G70 oat Aged Seeds.
As can be seen from Figure 4, compared with the control, epiphysin cause 12h, for 24 hours with 36h when, 1 μM, 10 μM, 100 μM, 200 μM,
The processing of 500 μM and 1000 μM each concentration is to the seedling root long of G52 and G70 oat Aged Seeds without significant (P >
0.05) improvement effect.
As can be seen from Figure 5, compared with the control, G52 oat Aged Seeds only cause 36h in epiphysin, and concentration is 1 μM, 10 μ
Seedling root restores significant (P < 0.05) and improves at M and 100 μM；And epiphysin when causing for 24 hours the processing of each concentration cannot show
It writes (P > 0.05) and improves its seedling root weight.But only when epiphysin causes for 24 hours, seedling root restores aobvious to G70 oat Aged Seeds
It writes (P < 0.05) to improve, and causing concentration is 100 μM and 200 μM；And each concentration is handled not when epiphysin causes 36h
Significantly (P > 0.05) its seedling root weight can be improved.
As can be seen from Figure 6, compared with the control, only when epiphysin causes 36h, seedling seedling length is shown G52 oat Aged Seeds
It writes (P < 0.05) to improve, and causing concentration is 1 μM and 500 μM；And epiphysin is when causing for 24 hours, the processing of each concentration cannot be shown
The seedling seedling for writing (P > 0.05) raising G70 oat Aged Seeds is long.G70 oat Aged Seeds are when epiphysin causes for 24 hours, 1 μ
M, it is long that 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM each concentration energy significant (P < 0.05) improves seedling seedling；And epiphysin
When causing 36h, only 1 μM of concentration can significant (P < 0.05) to improve seedling seedling long.
As can be seen from Figure 7, compared with the control, when epiphysin is caused for 24 hours, 100 μM and significant (the P < of 200 μM of concentration energy
0.05) the seedling seedling weight of G52 and G70 oat Aged Seeds is improved；In addition, when epiphysin causes 36h, to G52 oat aging kind
For son, 1 μM, 10 μM, 100 μM, 200 μM, 500 μM and 1000 μM each concentration significantly (P < 0.05) can improve its seedling
Miao Chong, and only 1 μM and 10 μM of concentration are capable of the seedling seedling weight that significant (P < 0.05) improves G70 oat Aged Seeds.
As it can be observed in the picture that compared with the control, when G52 oat Aged Seeds are caused for 24 hours through epiphysin, only 100 μM of concentration
Epiphysin can significant (P < 0.05) improve its seedling vigor index, and when epiphysin causes 36h, 1 μM, 10 μM, 100 μM,
The epiphysin of 200 μM, 500 μM and 1000 μM concentration has significant (P < 0.05) raising effect to its seedling vigor index.G70
Only when epiphysin causes for 24 hours, seedling vigor index is significantly improved (P < 0.05) oat Aged Seeds, and causes concentration and be
1 μM, 100 μM, 200 μM, 500 μM and 1000 μM；And when epiphysin initiation 36h, each concentration processing cannot significant (P >
0.05) the seedling vigor index of G70 oat Aged Seeds is improved.
In conclusion epiphysin various concentration causes processing to oat Aged Seeds germination percentage, germination index, seed vitality
Index, root long, root weight, seedling are long, seedling weight is different with the function and effect of seedling vigor index, and causing length of time with epiphysin has
It closes, while being influenced by seed vitality level.Table 10 is that epiphysin initiation reaches remarkable result to oat Aged Seeds indices
Cause the time and cause concentration combination.
Table 10：Epiphysin, which causes, to be reached the initiation time of remarkable result to oat Aged Seeds indices and causes concentration
Combination
As known from Table 10, for the epiphysin of 1 μM of -1000 μM of concentration for 24 hours or under the 36h initiation time, G52 or G70 oat is old
Changing seed indices has a function and effect, but comprehensive two groups of G52 and G70 oat Aged Seeds the result shows that, 1 μM of epiphysin draws
Hair 36h is the suitable initiation conditions for handling G52 and G70 oat Aged Seeds.
The two factor variance statistical results that epiphysin causes processing oat Aged Seeds are as shown in table 11.
Table 11：Epiphysin causes two factor variance statistical results of processing oat Aged Seeds
As known from Table 11, epiphysin causes the time, epiphysin causes the time and causes the mutual work of concentration to G52 and G70 swallow
Wheat Aged Seeds germination percentage, germination index, Seed Integrated vigor Index, root long, root weight, seedling is long, seedling is heavy and the shadow of seedling vigor index
It rings and reaches extremely significant horizontal (P < 0.01).For G52 oat Aged Seeds, epiphysin cause concentration remove to seedling root weight,
The influence of Miao Chong and seedling vigor index not significant (P > 0.05) outside, to percentage of seedgermination, germination index, Seed Integrated vigor Index,
Root long and long all have significantly (P < 0.05) of seedling influence, and to percentage of seedgermination, germination index, Seed Integrated vigor Index and seedling
The influence of root long reaches extremely significant horizontal (P < 0.01).For G70 oat Aged Seeds, epiphysin causes concentration to seed
The influence of germination percentage, vitality index and seedling vigor index is not significant (P > 0.05), and to seed germination index, root long, root
Heavy, seedling length and seedling all have significant (P < 0.05) again to be influenced, and the influence to root long, root weight and seedling length reaches extremely significant level
(P < 0.01).As it can be seen that the epiphysin initiation time is to influence oat aging kind during epiphysin causes oat Aged Seeds
Son is sprouted and the key factor of growth of seedling.Cause in time range in the set epiphysin of this test, the long period causes
It may advantageously facilitate the sprouting of oat Aged Seeds and growth of seedling.Therefore, G52 and two kinds of G70 different germinations are handled according to epiphysin
The initiation time of horizontal oat Aged Seeds and initiation concentration results determine that the horizontal oat in 52%-70% range of germination is old
Changing seed uses epiphysin to cause the suitable condition of processing to cause time 36h, causes 1 μM of concentration.
Claims (4)
1. a kind of method for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin, which is characterized in that the method
For：Measure first uses concentration for 1 μM -1000 μM for the initial water content for trying oat Aged Seeds after water content adjusts
24 h-36 h of epiphysin solution immersion treatment, then will for examination oat Aged Seeds take out and wash with distilled water, finally return
It does to the initial water content for trying oat Aged Seeds；
It is 9%-11% through the water content water content adjusted for trying oat Aged Seeds；
The germination percentage for trying oat Aged Seeds is 52%-70%.
2. a kind of method for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin according to claim 1,
It is characterized in that, carrying out water content adjustment to for examination oat Aged Seeds using saturated salt solution balance static weighing method.
3. a kind of method for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin according to claim 1,
It is characterized in that, being 10% through the water content water content adjusted for trying oat Aged Seeds.
4. a kind of method for promoting the sprouting of oat Aged Seeds and growth of seedling using epiphysin according to claim 1,
It is characterized in that, using concentration is 1 μM of epiphysin solution to for trying 36 h of oat Aged Seeds immersion treatment.
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| CN107079631A (en) * | 2017-06-21 | 2017-08-22 | 四川农业大学 | A kind of method for significantly reducing broad bean Cd accumulation and promoting it to grow |
| CN109104932B (en) * | 2018-08-11 | 2020-12-18 | 山东省潍坊市农业科学院 | Pretreatment-before-sowing method for improving activity of aged asparagus seeds |
| CN110999592A (en) * | 2020-02-11 | 2020-04-14 | 山西农业大学 | Device and method for concentrated sulfuric acid delinting and vitality restoration of white sheep grass seeds |
| CN111903844B (en) * | 2020-08-07 | 2022-05-13 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving fermentation quality of silage and prepared feed |
| CN112385352A (en) * | 2020-11-23 | 2021-02-23 | 河北农业大学 | Method for enhancing drought resistance of cotton seeds by melatonin and application |
| CN113079738B (en) * | 2021-03-23 | 2022-05-20 | 浙江省农业科学院 | Seed priming method for improving oil crop seed vigor |
| CN115152771A (en) * | 2022-05-13 | 2022-10-11 | 中国科学院东北地理与农业生态研究所 | Application of melatonin in cultivation of suaeda glauca |
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| CN104718840A (en) * | 2015-02-13 | 2015-06-24 | 中国农业大学 | Method for promoting plant seed germination under cadmium stress |
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