CN106068779B - A method of causing seed high temperature resistant using epiphysin enhancing oat and stores - Google Patents
A method of causing seed high temperature resistant using epiphysin enhancing oat and stores Download PDFInfo
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- CN106068779B CN106068779B CN201610453578.1A CN201610453578A CN106068779B CN 106068779 B CN106068779 B CN 106068779B CN 201610453578 A CN201610453578 A CN 201610453578A CN 106068779 B CN106068779 B CN 106068779B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01F—PROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
- A01F25/00—Storing agricultural or horticultural produce; Hanging-up harvested fruit
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- Health & Medical Sciences (AREA)
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Abstract
The invention belongs to seeds to sprout and storage technique field, and in particular to a method of it utilizes epiphysin enhancing oat to cause seed high temperature resistant and stores.The following steps are included: (1) selection is for trying oat seed；(2) adjustment of seed moisture content；(3) seed priming is handled；(4) simulation high-temperature storage processing；(5) epiphysin causes the determination of seed high temperature resistant holding conditions.The present invention can be used as the effective ways that enhancing oat causes seed high temperature resistant storage performance, promotes seed sprouting and growth of seedling.
Description
Technical field
The invention belongs to seeds to sprout and storage technique field, and in particular to a kind of to utilize epiphysin enhancing oat initiation kind
The method of sub- high temperature resistant storage.
Background technique
Seed priming is a kind of preconditioning technique that control seed slowly absorbs water with gradually Hui Gan.In practical agricultural production
In, often using the aged seed stored, and initiation processing can make the vigor of aged seed be restored or improve, and promote low
Seed under the adverse environmental factors such as temperature, arid is sprouted, and seed dosage is saved, and lowers production cost.It is unfavorable for seed storage however, causing
Hiding shortens seed longeivity, and then constrains its application in seed commodity production and sale.Currently, both at home and abroad about initiation
The research of technology and it is concentrated on using majority vegetables and Vigor of Crop are improved and improve aspect, rarely have and be related to causing pair
Seed longeivity, storage potential and the research for causing seed sprouting and growth of seedling influence aspect.
Epiphysin (Melatonin, MT) is a kind of indole derivatives of tryptophan, is found in earliest 1917,
The intracorporal physiological action of animal has been furtherd investigate.In recent years, epiphysin was detected in more and more plants,
It is also increasingly paid close attention to the functional study of plant growth and development and metabolism by people.Existing research shows epiphysin in plant
With removing free radical, adjust the physiological functions such as photoperiod, protection chlorophyll, enhancing stress resistance of plant.However, so far,
It is showed no both at home and abroad about epiphysin to the report for causing the storage of seeds service life and Seed Germination.
Oat (Avena sativa L.) is the annual Grain feed plant of grass family Avena, is mainly produced in China
In Qinghai, Gansu and Sichuan.High quality grass material of the oat as livestock and poultry, leaf and stalk succulence are tender, and palatability is good, thick egg
It is white, crude fat content is high, nutritive value is high, and crude fiber content is low, green hay, but also modulated ensilage can be not only made, to solution
Certainly extremely frigid zones domestic animal forage grass demand supplies seasonal uneven contradiction with forage grass and is of great significance.Meanwhile it is oat drought resisting, anti-
The inverse strong feature of property determines that it can adapt to the adverse circumstances of arid area.In addition, as green nourishing health care crop, oat
Contain a large amount of protein, soluble dietary fiber, vitamin and mineral in seed, fat content is higher, and linoleic acid content
Height, it is most important to human health.
In China, with the fast development of grassland agriculture and the continuous expansion of raising dairy cattle scale, Oatplanting area
Also it increases considerably, therefore the demand substantial increase of high-quality oat seed.Elicitation technique is as a kind of improvement seed vitality
And the effective ways of seedling growth, it is of great significance to seed Seed practical value is improved.It is unfavorable for seed storage however, causing
Hiding shortens seed longeivity, constrains its application in seed commodity production and sale, and then send out forage grass production and animal husbandry
Exhibition adversely affects.Therefore, it is used as the shortage that enhancing oat causes seed high temperature resistant storage technique, research for epiphysin
And determine that epiphysin enhancing oat causes the Water Content Conditions and operation sequence of the storage of seed high temperature resistant, it is of great significance.
Summary of the invention
The present invention provides a kind of method for causing the storage of seed high temperature resistant using epiphysin enhancing oat, specific steps
Are as follows:
(1) it selects for trying oat seed: being 100% by germination percentage using normal seedling as criterion calculation percentage of seedgermination
High activity level oat seed is used as trying oat seed；
(2) adjustment of seed moisture content: according to seed initial water content and seed aqueous amount adjustment method, in seed safety
It stores in water content ranges, 7%, 10% and 13% will be adjusted to for the water content for trying oat seed；
(3) seed priming is handled: being caused using 100-300 μM of epiphysin for trying oat seed 4-8h；
(4) simulation high-temperature storage processing: using the method simulation seeds high-temperature storage processing of control deterioration, by different water cut
The oat of amount causes seed and is placed in progress deterioration processing in 45 DEG C of high constant water bath box；
(5) epiphysin causes the determination of seed high temperature resistant holding conditions: causing seed in different moisture content item according to oat
Simulation high-temperature storage treated germination percentage, seedling length, Miao Chong, seedling vigor index variation is carried out under part, in conjunction with statistical analysis,
Determine that epiphysin enhancing oat causes the appropriate aqueous amount condition of seed high temperature resistant storage.
It preferably, is 7% or 10% for the water content for trying oat seed.
When carrying out deterioration processing in step (4), the oat of water content 7%, 10% and 13% causes seed and distinguishes high temperature
Handle 40d, 20d and 12d.
Preferably, concentration is used to handle 6h to for examination oat seed for 200 μM of epiphysin solution；Cause seed high temperature resistant
The appropriate aqueous amount of storage is 7% or 10%.
The invention has the following advantages:
(1) present invention can be used as enhancing oat and cause seed high temperature resistant storage performance, promote seed sprouting and seedling raw
Long effective ways.On the one hand, test determined oat cause seed under the high temperature conditions safe storage water content be 7%,
It is that change of moisture content a series of within the scope of 7%-13% are carried out with analysis and comparison result；On the other hand, test has determined
Under 10% Water Content Conditions, epiphysin can make the oat initiation resistance to 45 DEG C of high-temperature storages of seed extend to 12d, cause oat kind than water
The sub- more 4d of high temperature resistant storage time.Therefore, the performance that oat causes the storage of seed high temperature resistant can be enhanced using this technology, promoted
Cause seed sprouting and growth of seedling.
(2) it establishes the standardized operation sequence of this technology and advantageously accounts for what elicitation technique faced in production application
Problem.
Detailed description of the invention
Fig. 1 is the influence schematic diagram that epiphysin causes percentage of seedgermination to different moisture content oat；
Fig. 2 is that epiphysin causes the long influence schematic diagram of seed seedling seedling to different moisture content oat；
Fig. 3 is the influence schematic diagram that epiphysin causes seed seedling Miao Chong to different moisture content oat；
Fig. 4 is the influence schematic diagram that epiphysin causes seed seedling vigor index to different moisture content oat.
In the accompanying drawings, under the identical deterioration time, different lowercases indicate significant difference (P < 0.05) between C, HP and MP.
Specific embodiment
The present invention is described further combined with specific embodiments below, but protection not thereby limiting the invention
Range.Method flow
1.1 for examination oat seed material selection
For the practicability for ensuring to cause seed high temperature resistant storage research and application, sent out by criterion calculation seed of normal seedling
The method of bud rate come determine seed vitality level, select germination percentage be 100% seed (initial water content 8.9%) as examination
Material.
The measurement of 1.2 seed moisture contents
Referring to the 9th chapter of international Seed Inspection association (ISTA, 2015) Seed Inspection regulation, seed moisture content is measured.It weighs
Clean seed about 4.5g weighs (being accurate to 0.001g) after being put into sample box, and two repetitions are arranged.It, will at 130 DEG C -133 DEG C
Sample box cover is put into drying in oven 2h after opening.After time as defined in reaching, sample box cover is covered, is put into drier cooling
It is re-weighed after 30min.It is calculated by formula seed moisture content (MC), i.e. seed moisture content (%)=(M2-M3)/(M2-M1)×
100%.
M1: the weight (g) of sample box and lid；
M2: weight (g) before the baking of sample box and lid and sample；
M3: weight (g) after the baking of sample box and lid and sample.
The adjustment of 1.3 seed moisture contents
According to seed initial water content, kind corresponding when seed moisture content is adjusted to 7%, 10% and 13% is calculated
Sub- weight.Using the method for saturated salt solution balance static weighing, oat seed is put into and fills saturation MgCl2, NaBr and KBr
The closed container inner equilibrium 7d of saturated solution (equilibrium relative humidity (ERH) is respectively 33%, 57% and 80%) weighs seed weight repeatedly
Amount, until seed reaches weight corresponding to specified moisture content.
The processing of 1.4 seed primings
Oat seed is used to 15mL distilled water (H respectively2O it) is handled under 20 DEG C of dark conditions with 200 μM of epiphysin solution
6h (is denoted as HP and MP) respectively, seed weight: liquor capacity (w/v)=1:3, not cause processing as control (being denoted as C).It will kind
Son takes out, and distilled water flushing 3 times, filter paper blots the surface of the seed moisture, 20 DEG C, under 33%RH dark condition natural air drying adjust to
Seed moisture content is 7%, 10% and 13%.After reaching specified moisture content, seed is packed into immediately close in 9cm × 13cm aluminium foil bag
Envelope, 200 every bag.
1.5 simulation high-temperature storage processing
Using the method simulation seeds high-temperature storage processing of control deterioration.The oat of different moisture content is caused seed to be placed in
Deterioration processing is carried out in 45 DEG C of high constant water bath box.7%, 10% and 13% water content initiation seed difference high-temperature process 40d,
20d and 12d, and it is primary every 8d, 4d and 2d sampling respectively, in case used in test.
The measurement of 1.6 germinations
It is provided referring to ISTA (2015), chooses uniformly full 50, consistent seed, put it into and contain the filter paper that haves three layers
In 11cm × 11cm Petri dish, filter paper is soaked with 10mL distilled water, 20 in illumination box (GXZ-380B-LED)
It is cultivated under the conditions of DEG C illumination 8h, dark 16h.Using normal seedling as germination standard, the hair after 10d germination is calculated
Bud rate.
Germination percentage (G)=(G10/ N) × 100%, wherein G10For preceding 10d, all normal seedling number, N are sub total for planting experimentally
Number.
The measurement of 1.7 seedling growths
It is carried out according to germinating method in 1.6, using normal seedling as germination standard, after 10d germination, survey
Root long, seedling length and the Miao Xianchong of fixed all normal seedling, calculate seedling vigor index (SVI).
Seedling vigor index (SVI)=(ARL+ASL) × G, wherein ARL is average root long (cm), and ASL is that average seedling is long
(cm)。
1.8 data processings and analysis
Test data carries out conspicuousness statistical analysis using Excel2010 and SPSS17.0 software, uses
The mapping of SigmaPlot10.0 software.As a result it is indicated with average value ± standard error.
Embodiment 1
1. for examination oat seed selection
The present invention selects the oat seed sample of germination percentage 100%, and kind herdsman originates in the U.S., seed lot in 2014
Number Y16-14-CC-10.Essential information for trying oat seed sample is shown in Table 1.
Table 1 is for trying oat seed essential information
2. seed moisture content measurement and adjustment
Referring to international the 9th chapter of Seed Inspection association (ISTA, 2015) Seed Inspection regulation regulation, seed moisture content is measured
(MC)。
According to the initial water content of seed, calculate corresponding when seed moisture content is adjusted to 7%, 10% and 13%
Seed weight.Using the method for saturated salt solution balance static weighing, oat seed is put into and fills saturation MgCl2, NaBr and
The closed container inner equilibrium 7d of KBr saturated solution (equilibrium relative humidity (ERH) is respectively 33%, 57% and 80%), weighs seed repeatedly
Weight, until seed reaches weight corresponding to specified moisture content.
3. seed priming is handled
Oat seed is used to 15mL distilled water (H respectively2O it) is handled under 20 DEG C of dark conditions with 200 μM of epiphysin solution
6h, seed weight: liquor capacity (w/v)=1:3, not cause processing as control.Seed is taken out, distilled water flushing 3 times, is filtered
Paper blots the surface of the seed moisture, and 20 DEG C, natural air drying is adjusted to seed moisture content under 33%RH dark condition is 7%, 10% and
13%.After reaching specified moisture content, seed is packed into 9cm × 13cm aluminium foil bag immediately and is sealed, 200 every bag.
4. simulating high-temperature storage processing
The oat of different moisture content is caused into seed and is placed in progress deterioration processing in 45 DEG C of high constant water bath box.7%, 10%
High-temperature process 40d, 20d and 12d are distinguished with the initiation seed of 13% water content, and primary every 8d, 4d and 2d sampling respectively, with
Used in standby test.
5. epiphysin causes seed sprouting and the influence of growth of seedling to different moisture content oat
5.1 epiphysins cause the influence that seed is sprouted to different moisture content oat
For 7%MC oat seed during 40d high temperature deterioration, the germination percentage of HP and MP initiation seed is poor compared with compareing C
Different not significant (P > 0.05), is held in 100% (Fig. 1).
For 10%MC oat seed during 20d high temperature deterioration, control C percentage of seedgermination is always held at 100%, and HP
It is reduced with the MP germination percentage for causing seed, and drops to 5% or less in deterioration 20d；When high temperature deterioration 4d and 8d, HP causes
The germination percentage of seed significant (P < 0.05) lower than control C, respectively 96% and 77%, and MP cause the germination percentage of seed with it is right
It is not significant (P > 0.05) compared to difference according to C, respectively 99% and 94%, and MP causes the germination percentage of seed when high temperature deterioration 8d
Significantly (P < 0.05) is higher than the germination percentage that HP causes seed；When high temperature deterioration 12d and 16d, the germination percentage of HP and MP initiation seed
Significant (P < 0.05) is lower than control C, and MP causes the germination percentage (respectively 70% and 25%) of seed significantly (P < 0.05)
Higher than the germination percentage (respectively 30% and 10%) that HP causes seed.
After 13%MC oat seed high temperature deterioration 8d, control C percentage of seedgermination is reduced to 66% by 100%, and HP and MP
The germination percentage for causing seed is reduced to 0%；When high temperature deterioration 2d, the germination percentage of HP and MP initiation seed inequality compared with compareing C
Different not significant (P > 0.05), respectively 90% and 96%；When high temperature deterioration 4d-8d, the germination percentage of HP and MP initiation seed is aobvious
(P < 0.05) being write lower than control C, and when deterioration 4d, the germination percentage difference of HP and MP initiation seed is not significant (P > 0.05), and
When deterioration 6d, the germination percentage significant (P < 0.05) that MP causes seed is higher than the germination percentage (Fig. 1) that HP causes seed.As it can be seen that
10%MC seed is during 8d-16d high temperature deterioration, and compared with water causes HP, epiphysin causes MP and is conducive to enhance seed pair
The tolerance of high temperature slows down the reduction of percentage of seedgermination.
The long influence of the seedling seedling that 5.2 epiphysins cause seed to different moisture content oat
For 7%MC oat seed during 40d high temperature deterioration, control C, HP and MP have caused the seedling Miao Changjun of seed
It reduces, 10.70cm, 10.57cm and 10.90cm (deterioration is dropped to by 11.53cm, 11.60cm and 11.55cm (deterioration 0d) respectively
40d), but the long equal difference compared with compareing C of the seedling seedling of HP and MP initiation seed is not significant (P > 0.05) (Fig. 2).
During 20d high temperature deterioration, the seedling seedling length of control C seed is dropped to 10%MC oat seed by 11.80cm
10.66cm, and the seedling seedling length that HP and MP causes seed drops to 3.41cm and 0.81cm by 10.75cm and 10.84cm respectively,
And 20d high temperature deterioration process HP and MP cause the seedling seedling long significant (P < 0.05) of seed lower than control C；High temperature deterioration 4d
When with 8d, the seedling seedling that the seedling seedling long significant (P < 0.05) that MP causes seed is higher than HP initiation seed is long；Deterioration 12d and 16d
When, difference is not significant (P > 0.05) between the seedling seedling of HP and MP initiation seed is long.
After 13%MC oat seed high temperature deterioration 8d, the seedling seedling of control C seed is long to be reduced to 8.49cm by 11.58cm,
And the seedling Miao Changjun that HP and MP causes seed is reduced to 0cm (Fig. 2)；When high temperature deterioration 4d and 6d, MP causes the seedling seedling of seed
The seedling seedling that long significant (P < 0.05) is higher than HP initiation seed is long.
5.3 epiphysins cause the influence of the seedling Miao Chong of seed to different moisture content oat
For 7%MC oat seed during 40d high temperature deterioration, control C, HP and MP have caused the seedling Miao Chong of seed
Decline, reduces 0.388g, 0.314g and 0.188g (Fig. 3) respectively.
For 10%MC oat seed during 20d high temperature deterioration, control C, HP and MP cause the seedling Miao Chong difference of seed
Reduce 0.180g, 3.617g and 3.750g, when deterioration 4d, HP and MP cause not significant (the P > of difference between the seedling Miao Chong of seed
0.05), and when high temperature deterioration 8d-16d, the seedling Miao Chong significant (P < 0.05) that MP causes seed is higher than the children that HP causes seed
Miao Miao weight.
During 8d high temperature deterioration, the seedling Miao Chong that control C, HP and MP cause seed drops 13%MC oat seed respectively
Low 2.259g, 4.526g and 4.434g, when deterioration 2d, HP and MP cause not significant (the P > of difference between the seedling Miao Chong of seed
0.05), and when high temperature deterioration 4d-6d, the seedling Miao Chong significant (P < 0.05) that MP causes seed is higher than the seedling that HP causes seed
Miao Chong.
5.4 epiphysins cause the influence of the seedling vigor index of seed to different moisture content oat
For 7%MC oat seed during 40d high temperature deterioration, the seedling vigor index that control C, HP and MP cause seed is equal
Declined, reduce 167.075,147.565 and 63.715 (Fig. 4) respectively, MP causes the children of seed when except deterioration 24d-32d
Seedling vitality index significant (P < 0.05) is higher than HP and causes outside seedling vigor index of seed, and HP and MP draws when other deterioration times
Send out seed seedling vigor index with compare C difference not significantly (P > 0.05).
For 10%MC oat seed during 20d high temperature deterioration, control C, HP and MP cause the seedling vigor index of seed
Decline, reduces 302.600,1941.900 and 1949.500 (Fig. 4) respectively；During deterioration 4d-16d, HP and MP cause
The seedling vigor index of seed significant (P < 0.05) is lower than the seedling vigor index for compareing C seed, but MP causes the children of seed
Seedling vitality index significant (P < 0.05) is higher than the seedling vigor index that HP causes seed.
For 13%MC oat seed during 8d high temperature deterioration, the seedling vigor index that control C, HP and MP cause seed is equal
Decline, reduces 1006.190,2119.540 and 2057.750 (Fig. 4) respectively；MP causes the seedling vigor of seed when deterioration 4d
Index significant (P < 0.05) is higher than the seedling vigor index that HP causes seed.
In conclusion HP and MP cause seed and are resistant to high-temperature storage 40d, and germination percentage can be kept under the conditions of 7%MC
100%；Under the conditions of 10%MC, HP causes seed and is resistant to high-temperature storage 8d, germination percentage 77%, and MP causes seed and is resistant to height
Temperature storage 12d, germination percentage 70%, while during 8d-12d high temperature deterioration, compared with water causes HP, epiphysin, which causes MP, to be had
Conducive to enhancing seed to the tolerance of high temperature, slow down the reduction of percentage of seedgermination；Under the conditions of 13%MC, HP and MP initiation seed are equal
It is resistant to high-temperature storage 4d, germination percentage is respectively 64% and 72%.Therefore, in seed safety storage water content ranges, cause kind
The water content of son is lower, and it is longer to cause the storage time of seed under the high temperature conditions；Compared with 10%MC and 13%MC, 7%MC
It is more advantageous to the high-temperature storage that oat causes seed.In addition, seed longeivity can be reduced although causing the storage for being unfavorable for seed,
But compared with water causes, epiphysin causes the high temperature resistant Storage for being more advantageous to enhancing seed.
Claims (2)
1. a kind of method for causing the storage of seed high temperature resistant using epiphysin enhancing oat, which is characterized in that specific steps are as follows:
(1) select for trying oat seed: using normal seedling as criterion calculation percentage of seedgermination, the height for being 100% by germination percentage is living
The horizontal oat seed of power is used as trying oat seed；
(2) adjustment of seed moisture content: 7%, 10% and 13% will be adjusted to for the water content for trying oat seed；
(3) seed priming is handled: being caused using 100-300 μM of epiphysin for trying oat seed 4-8h；
(4) simulation high-temperature storage processing: the oat initiation seed of different moisture content is placed in 45 DEG C of high constant water bath box and is carried out
Deterioration processing；The oat that water content is 7%, 10% and 13% causes seed and distinguishes high-temperature process 40d, 20d and 12d；
(5) according to oat cause seed carried out under the conditions of different moisture content simulation high-temperature storage treated germination percentage, seedling it is long,
Miao Chong, seedling vigor index variation determine that epiphysin enhancing oat causes the suitable of seed high temperature resistant storage in conjunction with statistical analysis
Suitable Water Content Conditions are 7% or 10%, are then stored.
2. the method according to claim 1, wherein using concentration is 200 μM of epiphysin solution to for trying swallow
Wheat seeds subprocessing 6h；The appropriate aqueous amount for causing the storage of seed high temperature resistant is 7% or 10%.
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| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
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| CN104412979A (en) * | 2013-08-19 | 2015-03-18 | 中国科学院遗传与发育生物学研究所 | New applications of melatonin |
| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
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| 褪黑素与植物抗逆性研究进展;姜超强 祖朝龙;《生物技术通报》;20151231;第31卷(第4期);第47-51页 |
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