CN104412979A - New applications of melatonin - Google Patents
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- CN104412979A CN104412979A CN201310361933.9A CN201310361933A CN104412979A CN 104412979 A CN104412979 A CN 104412979A CN 201310361933 A CN201310361933 A CN 201310361933A CN 104412979 A CN104412979 A CN 104412979A
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Abstract
The invention discloses new applications of melatonin. One of the new applications of the melatonin is of an application of the melatonin in enhancement of salt tolerance of plants and promotion of growth of the plants. An experiment proves that by spraying seedlings with a melatonin solution with a molar concentration being 50-100 [mu]M, widths and areas of a first true leaf and a second true leaf of soybean seedlings can be significantly increased; by wrapping soybean seeds with the melatonin solution with the molar concentration being 50-100 [mu]M, the area of the first true leaf, plant height, and the area of a fifth true leaf can be significantly increased, number of pods, the number of seeds and weight of the seeds in a single plant of the soybean can be increased; and by wrapping the soybean seeds with the melatonin solution with the molar concentration being 50-100 [mu]M, biomass and leaf area of the soybean, under salt stress, can be significantly increased. The experimental results hereinbefore prove that the melatonin can be applied for increasing the biomass, yield and the salt tolerance of the soybean.
Description
Technical field
The present invention relates to the novelty teabag of epiphysin.
Background technology
In the plant husbandry of agricultural, often prior to seeding seed is wrapped up one deck chemical substance, to ensure the normal germination of seed.Just on the books in " book of agriculture victory " of China between Western Han Dynastry's year, with medicament seed soaking seed prior to seeding.The fifties in last century, the technology such as seed soaking, seed dressing of having promoted is for preventing and treating soil insect, and the normal growth of protection seed is grown.Through nearly 20 years of researches, the nineties, seed coat agent was developed to the stage of ripeness, was mainly used in corn, Wheat-cotton, paddy rice, soybean, on the crops such as vegetables.
Seed coat agent is for seed pelleting, a class preparation with film forming characteristics.Main employing adhesive or film forming agent, be coated on extraseminal preparation by agricultural chemicals or agricultural chemicals and chemical fertilizer etc., can make breeding standardization, has again the several functions such as plant protection effect.The benefit of seed pelleting is desinsection, sterilization, kills mouse; Change subenvironment around seed, provide nutrition and oxygen, coordinate plant growth, promote plant germination, growth and maturation etc.
Epiphysin, has another name called melatonin and melatonin, and chemical name is MLT, and molecular formula is C
13h
16n
2o
2, molecular weight is 232.28, structure as shown in Equation 1:
formula 1.
Epiphysin is present in the endocrinal glands pineal gland in mammalian brain, the generation of adjustable melanocyte agglutinin.Epiphysin is a kind of endogenous material, is worked in animal/mankind by the adjustment of endocrine system.In view of epiphysin and the mankind sleep relevant, be therefore usually used in the health medicine of improving water flood quality, in fact it also has significant immunoregulation effect.In view of its metabolic pathway of human body be the melatonin overwhelming majority in blood after liver metabolism becomes 6-hydroxyl melatonin sulphate form, excrete, fraction forms 6-hydroxyl melatonin Portugal candy thuja acid.Its biological half-life is short, and be namely down to the physiological level of normal person at oral 7 ~ 8h, therefore toxicity is minimum, is subject to the common people in recent years welcomes as health products.
Summary of the invention
Technical problem to be solved by this invention is to provide the novelty teabag of epiphysin.
Novelty teabag provided by the present invention is following 1) to 6) in any one:
1) epiphysin is improving the application in plant salt endurance and Promoting plant growth.
2) application in the product improving plant salt endurance and Promoting plant growth prepared by epiphysin.
3) epiphysin is improving the application in plant salt endurance.
4) epiphysin improves the application in the product of plant salt endurance in preparation.
5) application of epiphysin in Promoting plant growth.
6) application of epiphysin in the product preparing Promoting plant growth.
In above-mentioned application, described plant can be dicotyledon or monocotyledon.
In an embodiment of the invention, described plant is soybean.
In above-mentioned application, described epiphysin can the concentration of 50 μMs-100 μMs (as 50 μMs or 100 μMs) spray as described in the seed of plant or plant as described in wrapping up with the concentration of 50 μMs-100 μMs (as 50 μMs or 100 μMs).
In above-mentioned application, the period of spraying epiphysin to plant can be seedling stage.
In above-mentioned application, described raising plant salt endurance can be presented as following A 1) and/or A2:
A1) biomass of described plant under salt stress is improved;
A2) leaf area of described plant under salt stress is improved;
In a specific embodiment of the present invention, with 1%(plasmid percentage composition) NaCl aqueous solution analog salt coerces.
In above-mentioned application, described Promoting plant growth is presented as following B1) to B5) in all or part of:
B1) single-strain legumen number of soybean is improved;
B2) the single-strain seed number of soybean is improved;
B3) the single-strain seed weight of soybean is improved;
B4) leaf area of soybean is improved;
B5) plant height of soybean is improved.
In above-mentioned application, described product can be seed coat agent or plant growth promoter.Under described raising salt stress, the biomass of described plant specifically can be the plant height improving described plant under salt stress.
Experiment of the present invention proves, with 50 μMs-100 μMs epiphysin spray solution seedling, significantly can increase the rough leaf of soybean seedling and the width of second true leaf and leaf area; Significantly can increase the rough leaf area of soybean seedling and plant height and the 5th true leaf area with 50 μMs-100 μMs epiphysin solution parcel soya seeds, improve the single-strain legumen number of soybean, single-strain seed number and single-strain seed weight; The plant height of Soybean Under Salt Stress and the leaf area of soybean can be significantly improved with 50 μMs-100 μMs epiphysin solution parcel soya seeds.Illustrate that epiphysin can be applicable to increase the biomass of soybean, output and salt resistance.
Accompanying drawing explanation
Fig. 1 is that after variable concentrations epiphysin sprays, rough leaf width compares.
Fig. 2 is that the rear second true leaf width of variable concentrations epiphysin sprinkling compares.
Fig. 3 be soya seeds parcel seed coat agent and the plant height of contrast and leaf area ratio comparatively.
Fig. 4 is that epiphysin process seed adds biomass.
Fig. 5 is the phenotype of epiphysin process seed plant after 6 weeks.
Fig. 6 is pod number and the seed number that epiphysin process seed adds soybean per plant.
Fig. 7 is epiphysin process and adjoining tree 100-grain weight statistics.
Fig. 8 is that under salt stress, seed coat agent wraps up seed to the impact of plant strain growth
Fig. 9 is the phenotype of contrast and epiphysin different disposal plant under salt stress.
In Fig. 1-Fig. 2,0 μM represents the process of sprinkling distilled water, and 25 μMs represent sprinkling 25 μMs of epiphysin solution-treated, and 50 μMs represent sprinkling 50 μMs of epiphysin solution-treated, and 100 μMs represent sprinkling 100 μMs of epiphysin solution-treated.
In Fig. 3 and Fig. 8, one group of experimental plants contrast is for seed is without any process, and another group is for seed is through the process of parcel seed coat agent.
In Fig. 4-Fig. 7 and Fig. 9,0 represents that seed coat agent parcel process, 50 represents 50 μMs of epiphysins/seed coat agent mixed liquor parcel process, and 100 μMs represent 100 μMs of epiphysins/seed coat agent mixed liquor parcel process.
Embodiment
Below in conjunction with embodiment, the present invention is further described in detail, the embodiment provided only in order to illustrate the present invention, instead of in order to limit the scope of the invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Epiphysin (MLT) in following embodiment eats Chemical Co., Ltd. purchased from Guangzhou Kang.
Quantitative test in following examples, all arranges and repeats experiment for three times, and data are the mean value or mean+SD that repeat for three times to test.
Soybean in following embodiment is pacified agriculture 28, from peaceful agriculture No. 14 variants, forms through pedigree method seed selection.2006 by Heilongjiang Province's crop varietal approval committee.Authorization numbering: black careful beans 2006002.Seed selection unit: Suihua branch of Heilongjiang Institute of Agricultural Sciences contact address: No. 420, Suihua City workers and peasants West Road, Heilongjiang Province postcode: 152052 contact persons: ginger becomes happiness e-mail: chengxijiang@126.com.Website http://www.farmers.org.cn/Article/ShowArticle.asp Article ID=74257.
The agriculture 28 of pacifying of soybean in following embodiment is all grown on 22 DEG C, and the illumination condition of every day is that 16h illumination/8h is dark).
The seed coat agent that following all embodiments adopt is Bayer Bitterfeld GmbH--high skilful 60% Imidacloprid suspension seed-coating agent (50ml packaging).Manufacturing enterprise's agriculture chemical registration card number: PD20050056, packing enterprise agriculture chemical registration card number: PD20050056F070087, product standard card number: Q/3201SKSH026-2010.Production standard card number: HNP32153-P0475.Its feature is pest control, comprises grub, soil insect, thrips, aphid, wireworm, leafhopper and plant hopper.
Embodiment 1, epiphysin promote soybean leaves growth experiment
Take absolute ethyl alcohol as solvent, preparation 30mM epiphysin mother liquor, adds distilled water afterwards, prepares 25,50 and 100 μMs of epiphysin solution respectively.Soybean is pacified after agriculture 28 soya seeds germinates one week, and spray distilled water, 25,50 and 100 μMs of epiphysin solution respectively, 2ml/, 2 days once, sprays 3 times altogether.Each concentration sprays 20 plant, in triplicate.Fig. 1 is the phenotype of seedling (spraying 2 epiphysin solution altogether) and the width statistics of rough leaf of germinateing latter 10 days.Fig. 2 is phenotype and second true leaf width statistics of the seedling (spraying 3 epiphysin solution altogether) germinateed latter 28 days.
Experimental result shows, with 50 μMs-100 μMs epiphysin spray solution seedling, significantly can increase the rough leaf of seedling and the width of second true leaf and leaf area.Spray 50 μMs and 100 μMs of epiphysins do not have significant difference.
Embodiment 2, epiphysin parcel seed promotes soybeans they grow experiment
Owing to sowing with after seed coat agent parcel seed in Soybean production, therefore in this experiment, first carrying out seed through wrapping up the growth fraction of seed coat agent process and untreated plant comparatively, can soybeans they grow have been improved to study seed coat agent.Setup Experiments two groups, does not wrap up seed coat agent processed group (seed not wrapping up seed coat agent is sowed) and parcel seed coat agent processed group (seed of parcel seed coat agent is sowed), and often group to be pacified agriculture 28 seedling containing 30 soybean.All soybean are pacified and to plant after agriculture 28 seed sprouting in being in the flowerpot of 1:1 containing vermiculite and Nutrition Soil.Fig. 3 shows the growing state of 14 days seedlings after seed coat agent parcel and the seed sprouting of not wrapping up and plant height and leaf area statistics (in Fig. 3, contrast as not wrapping up seed coat agent processed group, seed parcel seed coat agent is parcel seed coat agent processed group).The plant height not wrapping up seed coat agent processed group and parcel seed coat agent processed group seedling is about 15.0 and 14.3cm respectively, and leaf area is about 15.2 and 13.4cm respectively
2, the growth after seed coat agent process seed is slightly inferior to the growth of untreated seed, but does not present significant difference.Above-mentioned experiment shows, the use of seed coat agent does not improve soybeans they grow, and therefore following experiment no longer arranges the contrast not doing seed coat agent process.
Then, test epiphysin as follows and can promote soybeans they grow: to pacify agriculture 28 seed with seed coat agent and epiphysin mixing parcel soybean.Take absolute ethyl alcohol as solvent, preparation 30mM epiphysin mother liquor.Epiphysin mother liquor is added and is 50 μMs to epiphysin concentration in above-mentioned seed coat agent and obtains 50 μMs of epiphysins/seed coat agent mixed liquor, epiphysin mother liquor is added and is 100 μMs to epiphysin concentration in above-mentioned seed coat agent and obtains 100 μMs of epiphysins/seed coat agent mixed liquor.
3 process are established in epiphysin experiment, are respectively: the process of seed coat agent parcel, 50 μMs of epiphysin/seed coat agent mixed liquor parcel process, 100 μMs of epiphysin/seed coat agent mixed liquor parcel process.Three repetitions are all established in each process, and each reprocessing 30 soybean are pacified agriculture 28 seed, and the pacify size of agriculture 28 seed of soybean of each process is similar.With above-mentioned seed coat agent parcel seed in the process of seed coat agent parcel, with 50 μMs of epiphysins/seed coat agent mixed liquor parcel seed in 50 μMs of epiphysin/seed coat agent mixed liquor parcel process, with 100 μMs of epiphysins/seed coat agent mixed liquor parcel seed in 100 μMs of epiphysin/seed coat agent mixed liquor parcel process, after drying, (about needing 3-5 minute), all seeds are planted in the flowerpot containing 150 grams of vermiculites and 150 grams of Nutrition Soils simultaneously, 2, every basin.Fig. 4 shows the germinate latter 14 days growing states through epiphysin process and untreated plant and rough leaf area and plant height statistics.The mean value of the rough leaf area of the process of seed coat agent parcel, 50 μMs of epiphysins/seed coat agent mixed liquor parcel process and 100 μMs of epiphysin/seed coat agent mixed liquor parcel process plant is respectively 8.7,14.0 and 14.7cm
2, the mean value of plant height is respectively 21,27 and 26cm; 50 μMs of epiphysins/seed coat agent mixed liquor parcel process and 100 μMs of epiphysin/seed coat agent mixed liquors parcel process are all and seed coat agent wraps up the rough leaf area between processing and plant height all presents significant difference, sow the plant growing way difference after 6 weeks more remarkable, blade and the plant height of all epiphysin process plant are all greater than contrast.Added up the area that seed coat agent parcel processes, 50 μMs of epiphysin/seed coat agent mixed liquors wrap up the 5th true leaf of process and 100 μMs of epiphysin/seed coat agent mixed liquor parcel process plant, its mean value is respectively 28,42 and 43cm
2(Fig. 5).Maturation, calculates single-strain legumen number, single-strain seed number, single-strain seed weight and 100-grain weight after results.Fig. 6 shows, the mean value of the average pod number of individual plant of the process of seed coat agent parcel, 50 μMs of epiphysins/seed coat agent mixed liquor parcel process and 100 μMs of epiphysin/seed coat agent mixed liquor parcel process plant is respectively 12.5,17 and 22, the mean value of single-strain seed number is respectively 23,34 and 40, and the mean value that single-strain seed is heavy is respectively 5.8,8.0 and 9.0 grams.The process of seed coat agent parcel, 50 μMs of epiphysin/seed coat agent mixed liquor parcel process and 100 μMs of epiphysin/seed coat agent mixed liquors wrap up the 100-grain weight of process plant without significant difference, are all about 25 grams (Fig. 7).
The above results shows, and significantly can increase true leaf area and the plant height of soybean seedling, improve single-strain legumen number, single-strain seed number, the single-strain seed weight of soybean, add biomass and the seed production of plant with 50 μMs-100 μMs epiphysin solution parcel soya seeds.
Embodiment 3, epiphysin process seed add the salt resistance of plant
First the impact of seed coat agent on plant salt resistance is identified.Experiment is divided into 4 groups, do not wrap up seed coat agent water planting processed group (seed not wrapping up seed coat agent is sowed) and parcel seed coat agent water planting processed group (seed of parcel seed coat agent is sowed), do not wrap up seed coat agent salt stress processed group (seed not wrapping up seed coat agent is sowed) and parcel seed coat agent salt stress processed group (seed of parcel seed coat agent is sowed), often group 30 soybean are pacified agriculture 28 seedling.
First by in-built mixed uniformly 150 grams of vermiculites and 150 grams of Nutrition Soils, onesize flowerpot is placed in the glass basin that 500ml water or the 1%NaCl aqueous solution are housed respectively, record water/salting liquid institute arrives highly, soil is made fully to draw water or salting liquid, being pacified by soybean after the seedling after agriculture 28 seed sprouting grows 7 days moves in above-mentioned flowerpot respectively, observe every day and keep water or salting liquid height, to keep NaCl concentration for 1%.Wherein, do not wrap up in seed coat agent water planting processed group and the glass basin of parcel seed coat agent water planting processed group all as dress water, do not wrap up in seed coat agent salt stress processed group and the glass basin of parcel seed coat agent salt stress processed group all as the dress 1%NaCl aqueous solution.Within 7 days, add up plant height and leaf area afterwards.The plant height not wrapping up seed coat agent water planting processed group and parcel seed coat agent water planting processed group is about 15.0 and 14.3cm respectively, and leaf area is about 15.2 and 13.4cm respectively
2, the plant height not wrapping up seed coat agent salt stress processed group and parcel seed coat agent salt stress processed group is about 7.3 and 6.3cm respectively, is respectively 48.7% and 44.1%, without significant difference with the relative plant height of water planting; Leaf area is about 5.0 and 3.8cm respectively
2, seed coat agent process seedling leaf area after salt stress obviously reduces, but leaf area relative to water planting is respectively 32.9% and 28.4%, and difference is (Fig. 8, contrasts as not wrapping up seed coat agent process, and seed coat agent is treated to the process of parcel seed coat agent) very not obviously.Above-mentioned experiment shows that seed coat agent process seed does not increase the salt resistance of large bean seedlings.
Then, test epiphysin as follows and can increase soybean salt-tolerance: 6 processed group are established in experiment, are respectively: seed coat agent parcel water planting processed group, seed coat agent parcel salt stress processed group, 50 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group, 50 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group, 100 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group, 100 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group.Seed is wrapped up by above-mentioned seed coat agent in seed coat agent parcel water planting processed group and seed coat agent parcel salt stress processed group, seed is wrapped up with the 50 μMs of epiphysins/seed coat agent mixed liquor of embodiment 2 in 50 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group and 50 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group, seed is wrapped up with the 100 μMs of epiphysins/seed coat agent mixed liquor of embodiment 2 in 100 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group and 100 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group, the seed that these six kinds process is germinateed simultaneously.First by in-built mixed uniformly 150 grams of vermiculites and 150 grams of Nutrition Soils, onesize flowerpot is placed in the glass basin that 500ml water or the 1%NaCl aqueous solution are housed respectively, record water/salting liquid institute arrives highly, soil is made fully to draw water/salting liquid, the normal growth large bean seedlings of 7 days after germination are moved in above-mentioned flowerpot respectively, observe every day and keep water/salting liquid height, to keep NaCl concentration for 1%.Three repetitions are all established in each process, eachly repeat often to organize process 30 soybean and to pacify agriculture 28 seed, and the pacify size of agriculture 28 seed of soybean of each process is similar.Wherein, seed coat agent parcel water planting processed group, 50 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group and 100 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group are all placed in the glass basin filling water; Seed coat agent parcel salt stress processed group, 50 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group and 100 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group are all placed in the glass basin filling the 1%NaCl aqueous solution.
Fig. 9 shows the phenotype respectively organizing plant when salt stress cultivates 7 days and 21 days, and the growth of 2 groups of (50 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group and 100 μMs of epiphysin/seed coat agent mixed liquor parcel salt stress processed group) plant of epiphysin process is obviously better than untreated fish group (seed coat agent parcel salt stress processed group).Salt stress has added up plant height and relative plant height percentage (salt stress cultivates plant height/Aquaponic plant height), the 3rd true leaf area and relative leaf area percentage (salt stress cultivation leaf area/Aquaponic leaf area) after 21 days, list in table 1.Seed coat agent parcel water planting processed group: the water planting plant height of 21 days is 61.5 ± 9.4cm, the plant height that seed coat agent parcel salt stress processed group salt stress is cultivated 21 days is 18.2 ± 5.4cm, and the relative plant height that salt stress is cultivated 21 days is 29.6 ± 6.2%; 50 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group water planting plant heights of 21 days are 76.6 ± 10.1cm, 50 μMs of plant heights that epiphysin/seed coat agent mixed liquor parcel salt stress processed group salt stress is cultivated 21 days are 30.2 ± 6.1cm, and the relative plant height that salt stress is cultivated 21 days is 39.4 ± 7.4%; 100 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group water planting plant heights of 21 days are 79.2 ± 9.8cm, 100 μMs of plant heights that epiphysin/seed coat agent mixed liquor parcel salt stress processed group salt stress is cultivated 21 days are 31.6 ± 5.1cm, and the relative plant height that salt stress is cultivated 21 days is 39.9 ± 6.8%; Seed coat agent parcel water planting processed group water planting the 3rd true leaf area of 21 days is 20.1 ± 5.5cm
2, the 3rd true leaf area that seed coat agent parcel salt stress processed group salt stress is cultivated 21 days is 1.8 ± 0.8cm
2, the 3rd relative leaf area of true leaf that salt stress is cultivated 21 days is 9.0%; 50 μMs of epiphysin/seed coat agent mixed liquor parcel water planting processed group water planting the 3rd true leaf areas of 21 days are 23.2 ± 8.2cm
2, 50 μMs of the 3rd true leaf areas that epiphysin/seed coat agent mixed liquor parcel water planting salt stress group salt stress is cultivated 21 days are 8.0 ± 3.9cm
2, the 3rd relative leaf area of true leaf that salt stress is cultivated 21 days is 34.5%; 100 μMs of epiphysins/seed coat agent mixed liquor parcel water planting processed group water planting the 3rd true leaf area of 21 days is the 3rd true leaf area that 25.5 ± 5.3,100 μMs of epiphysins/seed coat agent mixed liquor parcel water planting salt stress group salt stress is cultivated 21 days is 10.2 ± 4.3cm
2, the 3rd relative leaf area of true leaf that salt stress is cultivated 21 days is 40.0%.
The above results illustrates, significantly can increase the biomass (plant height and leaf area) of Soybean Under Salt Stress with 50 μMs-100 μMs epiphysin solution parcel soya seeds, illustrates with 50 μMs and 100 μMs of epiphysin parcel seeds, improves the salt resistance of plant.
Under table 1 salt stress contrast and epiphysin wrap up seed plant plant height and leaf area
Claims (10)
1. epiphysin is improving the application in plant salt endurance and Promoting plant growth.
2. the application in the product improving plant salt endurance and Promoting plant growth prepared by epiphysin.
3. epiphysin is improving the application in plant salt endurance.
4. epiphysin improves the application in the product of plant salt endurance in preparation.
5. the application of epiphysin in Promoting plant growth.
6. the application of epiphysin in the product preparing Promoting plant growth.
7., according to described application arbitrary in claim 1-6, it is characterized in that: described plant is dicotyledon or monocotyledon.
8., according to described application arbitrary in claim 1-7, it is characterized in that: described plant is soybean.
9., according to described application arbitrary in claim 1-8, it is characterized in that: described epiphysin sprays described plant with the concentration of 50 μMs-100 μMs or wraps up the seed of described plant with the concentration of 50-100 μM.
10., according to described application arbitrary in claim 1-9, it is characterized in that: described raising plant salt endurance is presented as following A 1) and/or A2:
A1) biomass of described plant under salt stress is improved;
A2) leaf area of described plant under salt stress is improved;
Described Promoting plant growth is presented as following B1) to B5) in all or part of:
B1) single-strain legumen number of soybean is improved;
B2) the single-strain seed number of soybean is improved;
B3) the single-strain seed weight of soybean is improved;
B4) leaf area of soybean is improved;
B5) plant height of soybean is improved.
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| CN104839160A (en) * | 2015-03-24 | 2015-08-19 | 安徽省农业科学院烟草研究所 | Drought-resistant maize soaking liquid and preparation method therefor |
| CN105076136A (en) * | 2015-07-13 | 2015-11-25 | 中国农业大学 | Novel application of melatonin in resistance to waterlogging stress of plants |
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