CN106069693A - A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced - Google Patents
A kind of compound adjusting control agent is in the application alleviated during Brassica campestris L salt damage is coerced Download PDFInfo
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- CN106069693A CN106069693A CN201610525765.6A CN201610525765A CN106069693A CN 106069693 A CN106069693 A CN 106069693A CN 201610525765 A CN201610525765 A CN 201610525765A CN 106069693 A CN106069693 A CN 106069693A
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- Prior art keywords
- melatonin
- sodium chloride
- brassinosteroid
- brassica campestris
- control agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses the compound adjusting control agent of a kind of melatonin and brassinosteroid in the application alleviated during Brassica campestris L salt damage is coerced, step is: a, test process: at rectangular plastic magazine attached last layer nylon wire, tightened with rubber band, be placed in the hydroponic box equipped with 1/4 nutritional solution, be arranged on nylon wire, it is placed in culturing room, light light culture, after select the Brassica campestris L seedling that growing way is consistent, snap in plastic plate with holes, root is immersed in culture fluid and cultivate, treat that Brassica Napus Seedling length is to true leaf;B, the mensuration of physical signs: after process, measure the plant height of rapeseed plants, leaf area, aerial parts and under ground portion fresh weight respectively, complete subsequently, dries and claims its dry weight to constant weight;C, data process;The application of the compound formulation of d, melatonin and brassinosteroid.This application promotes the growth of rapeseed plants and the accumulation (dry weight, underground dry weight on the ground) of dry, simple, simple to operate, nontoxic, good stability.
Description
Technical field
The invention belongs to agriculture plant production field, be more particularly to a plant growth regulators and alleviating Brassica campestris L salt
Evil coerce in application, the various physiological process of wide participation plant, it is possible to hestening rooting, promote growth, carry Seedling, strong sprout, keep a full stand of seedings,
Improving quality, improves yield, especially has important regulation effect at growth and development of plants and to aspects such as the responses of adverse circumstance.
This research takes the compound formulation applying melatonin and brassinosteroid to alleviate the salt stress infringement to Brassica campestris L, gives Brassica campestris L and supports
The ability of anti-poor environment.
Background technology
Crop is to be restricted by inherited character and two kinds of factors of crop body physiological situation the adaptation of adverse circumstance, the latter again with
Crop hormone in vivo close relation.Utilize plant growth regulator to strengthen the crop resistivity to adverse circumstance, it has also become
One of important channel of crop Adverse Resistance Cultivation at present.Salinized soil is a global problem to the threat of agricultural.For a long time,
The most concerned to the harm of plant about how solving the soil salinization.Brassica campestris L is as the big oil crop in third place in the world
Edible vegetable oil material crop big with China first, planting range is widely.Saline Land can make plant water suction difficulty, wilts
Lethal, affect plant nutrient and absorb, thus cause the underproduction.Therefore the salt tolerance studying Brassica campestris L, the salt resistance ability improving Brassica campestris L show
Obtain particularly significant.
Melatonin, as a kind of indole hormone, has many physiological functions in plant, mainly includes regulation
Photoperiod, removing free radical, protection chlorophyll etc., have been reported and point out, melatonin processes can effectively alleviate adverse circumstance environment pair
The depression effect of growth increment, the degraded slowing down leaf chlorophyll is run off, and maintains the photosynthetic efficiency that plant is higher, reduces adverse circumstance
The oxidative damage caused.Brassinosteroid is as a kind of new green environment protection plant growth regulator, it is possible to hestening rooting, carry
Seedling, strong sprout, volume increase, improve its oxidation resistance, thus improve the resistance of plant.Melatonin and brassinosteroid are all to adverse circumstance
There is certain relieving effect, but Brassica campestris L salt stress aspect is combined to the research the freshest understatement road of adjusting control agent.Outside the present invention applies
The compound adjusting control agent of source material melatonin and brassinosteroid alleviates the infringement that Brassica campestris L is caused by salt to a greater degree, for agricultural
The anti-salt of chemistry provides the new way of a safe noresidue.
Summary of the invention
It is an object of the invention to the compound adjusting control agent that there are provided a kind of melatonin and brassinosteroid and alleviate Brassica campestris L
Salt damage coerce in application (in Brassica campestris L salt damage is coerced promote plant growing thus alleviate the application of damage), promote Brassica campestris L and plant
The growth (root length, stem length, on the ground fresh weight, underground fresh weight) of strain and the accumulation (dry weight, underground dry weight on the ground) of dry, the most easily
OK, simple to operate, nontoxic, good stability.
In order to realize above-mentioned purpose, the present invention uses techniques below measure,
A kind of compound adjusting control agent, in the application alleviated during Brassica campestris L salt damage is coerced, the steps include:
1, test processes: at rectangle (22cm х 15cm х 7cm) plastic casing attached last layer nylon wire, with rubber band by it
Tighten, be placed in the hydroponic box (52cm х 32cm х 14cm) equipped with 1/4 nutritional solution, it is ensured that liquid level just touches nylon wire.
Selected full uniform seed, is arranged on nylon wire, is placed in 24-26 DEG C of culturing room, 16/8h light light culture 6-8d, then
Select the Brassica campestris L seedling that growing way is consistent, snap in plastic plate with holes, root is immersed Huo Gelan (Hoagland) culture fluid relaying
Continuous cultivation, after 4-6d when Brassica Napus Seedling length to 2 slice true leaf (Fig. 1), under condition of salt stress, carries out following 10 kinds of process: right
According to (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L melatonin (N+M30), 0.75% sodium chloride+
45umol/L melatonin (N+M45), 0.75% sodium chloride+60umol/L melatonin (N+M60), 0.75% sodium chloride+75umol/
L melatonin (N+M75), 0.75% sodium chloride+0.1umol/L brassinosteroid (N+BR1), 0.75% sodium chloride+0.2umol/L
Brassinosteroid (N+BR2), 0.75% sodium chloride+0.3umol/L brassinosteroid (N+BR3), 0.75% sodium chloride+
0.4umol/L brassinosteroid (N+BR4), often processes 7-10 strain, repeats for 2-4 time, and 2-4d changes single treatment liquid.
2, the mensuration of physical signs: process after 8-10d, measure respectively the plant height of 7-10 strain rapeseed plants, leaf area,
Upper part and under ground portion fresh weight, subsequently in the 105-107 DEG C of 30-45min that completes, dry to constant weight for 80-85 DEG C and claim it to do
Weight.
3, data process: each process is carried out significant difference analysis, according to the root length of rapeseed plants, stem length, overground part
Point dry weight and fresh weight of plant seedlings and under ground portion dry weight and fresh weight of plant seedlings filter out optium concentration 30umol/L of melatonin and brassinosteroid the most respectively
Good concentration 0.2umol/L.
4, the application of the compound formulation of melatonin and brassinosteroid: under condition of salt stress, arranges following 4 kinds of process:
Comparison (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L melatonin (N+M30), 0.75% sodium chloride+
0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+30umol/L melatonin+0.2umol/L brassinosteroid (N+
M30+BR2), find that Brassica campestris L salt damage is coerced tool by the compound adjusting control agent of 30umol/L melatonin and 0.2umol/L brassinosteroid
There is significant mitigation, promote the growth of rapeseed plants.
The present invention compared with prior art, has the following advantages and effect:
The compound adjusting control agent of melatonin and brassinosteroid is applied to during Brassica campestris L salt damage coerces by the present invention, can significantly alleviate
The salt stress injury to rapeseed plants, hence it is evident that improve the ability of rapeseed plants opposing salt stress, thus reduce salt stress and caused
The decline of yield and quality.Comparing single adjusting control agent, effect is more preferable, promote to a greater degree rapeseed plants growth and
The accumulation of dry.
Accompanying drawing explanation
Fig. 1 is growing state schematic diagram before a kind of rapeseed plants processes.
The method utilizing water planting cultivates rapeseed plants, long to process during 2 true leaves.
Fig. 2 is alleviated salt damage for a kind of adjusting control agent and is coerced schematic diagram.
Can be seen that from figure, the applying of compound adjusting control agent substantially alleviates salt damage and coerces, and plant growth condition is substantially better than salt
The plant of harm reason.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further elaborated, but the present invention is not limited to following example.
During embodiment 1:(salt stress, the alleviation of growth of rape is applied by a kind of compound formulation)
The compound adjusting control agent of a kind of melatonin and brassinosteroid is in the application alleviated during Brassica campestris L salt damage is coerced, its step
It is:
1, test processes: at rectangle (22cm х 15cm х 7cm) plastic casing attached last layer nylon wire, with rubber band by it
Tighten, be placed in the hydroponic box (52cm х 32cm х 14cm) equipped with 1/4 nutritional solution, it is ensured that liquid level just touches nylon wire.
Selected full uniform seed, is arranged on nylon wire, is placed in 25 DEG C of culturing room, 16/8h light light culture 6 or 7 or 8 days.So
After select the Brassica campestris L seedling that growing way is the same, snap in plastic plate with holes, root immersed in Huo Gelan (Hoagland) culture fluid
Continue to cultivate, after 4 or 5 or 6 days when Brassica Napus Seedling grows 2 true leaves (Fig. 1), under condition of salt stress, carry out following 10 kinds
Process: comparison (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L melatonin (N+M30), 0.75% chlorine
Change sodium+45umol/L melatonin (N+M45), 0.75% sodium chloride+60umol/L melatonin (N+M60), 0.75% sodium chloride+
75umol/L melatonin (N+M75), 0.75% sodium chloride+0.1umol/L brassinosteroid (N+BR1), 0.75% sodium chloride+
0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+0.3umol/L brassinosteroid (N+BR3), 0.75% chlorination
Sodium+0.4umol/L brassinosteroid (N+BR4), often processes 7 or 9 or 10 strains, repeats for 2 or 3 or 4 times, within 2 or 3 or 4 days, change one
Secondary treatment fluid.
2, the mensuration of physical signs: after processing 8 or 9 or 10 days, measures the plant height of 7 or 9 or 10 strain rapeseed plants, leaf respectively
Area, aerial parts and under ground portion fresh weight, subsequently in 105 or 106 or 107 DEG C of complete 28 or 29 or 30 or 31 or 32min, 78
Or 79 or 80 or 81 or 82 DEG C of drying claim its dry weight to constant weight.
3, data process: each process is carried out significant difference analysis, according to the root length of rapeseed plants, stem length, overground part
Point dry weight and fresh weight of plant seedlings and under ground portion dry weight and fresh weight of plant seedlings filter out optium concentration 30umol/L of melatonin and brassinosteroid the most respectively
Good concentration 0.2umol/L.
4, the application of the compound formulation of melatonin and brassinosteroid:
Test processing method according to above-mentioned steps 1,2, weigh melatonin, brassinosteroid, addition sodium chloride respectively to battalion
In nutrient solution, carry out following 5 kinds of process: comparison (CK1), 0.75% sodium chloride (CK2), 0.75% sodium chloride+30umol/L take off black
Element (N+M30), 0.75% sodium chloride+0.2umol/L brassinosteroid (N+BR2), 0.75% sodium chloride+30umol/L melatonin
In+0.2umol/L Brassica campestris L element (N+M30+BR2).
When often processing rapeseed plants 7 or 8 or 9 strain, repeat for 2 or 3 or 4 times, within 2 or 3 or 4 days, change single treatment liquid.Process 8
Or after 9 or 10 days, measure root length, stem length, aerial parts and the under ground portion fresh weight of 7 or 8 or 9 strain rapeseed plants respectively, subsequently
In 105 or 106 or 107 DEG C of complete 28 or 29 or 30 or 31 or 32min, dry for 78 or 79 or 80 or 81 or 82 DEG C and claim to constant weight
Its dry weight.Acquired results is all shown in Table 1.
The impact that rapeseed plants is grown by table 1 adjusting control agent
Note: different lower cases represent that between different disposal, difference reaches 5% significant level
As shown in Table 1, coercing lower rapeseed plants CK2 growing way at salt damage weak, root length, stem length, on the ground dry weight and fresh weight of plant seedlings and underground are dry
Weight average is substantially less than the comparison CK1 of normal growth, illustrates that salt stress significantly suppress the growth of plant, shows obvious salt
Evil symptom.Apply melatonin M30 and significantly improve the plant height of Brassica campestris L and ground dry weight and fresh weight of plant seedlings, root length and underground dry weight and fresh weight of plant seedlings under salt stress
Though increasing, but not up to significant level, illustrate that the mitigation of Brassica campestris L salt stress is focused on its aerial parts by melatonin.
Another aspect, applies brassinosteroid BR2 and significantly improves root length and the underground dry weight and fresh weight of plant seedlings of rapeseed plants, plant height and on the ground dry weight and fresh weight of plant seedlings
Slightly improve, but not up to significant level, illustrate that the mitigation of Brassica campestris L salt stress is focused on its under ground portion by melatonin.With
Time apply both adjusting control agents compared with applying single adjusting control agent, the fresh and dried weight average of root length, on the ground dry weight and fresh weight of plant seedlings and underground obtains
Significantly improve, farthest promote the growth of rapeseed plants.
In sum, in alleviating the application that Brassica campestris L salt damage is coerced, melatonin and the compound adjusting control agent ratio of brassinosteroid
Single adjusting control agent effect is more preferable, promotes the growth of rapeseed plants and the accumulation of dry to a greater degree, hence it is evident that improve
Salt damage coerces the upgrowth situation (Fig. 2) of lower rapeseed plants.
Claims (1)
1. the compound adjusting control agent of melatonin and brassinosteroid is in the application alleviated during Brassica campestris L salt damage is coerced, and the steps include:
A, test process: at rectangular plastic magazine attached last layer nylon wire, tightened with rubber band, be placed in equipped with 1/4 nutrition
In the hydroponic box of liquid, liquid level touches nylon wire, selects full seed, is arranged on nylon wire, is placed in 24-26 DEG C of culturing room
In, 16/8h light light culture 6-8d, then selects the Brassica campestris L seedling that growing way is consistent, snaps in plastic plate with holes, immersed suddenly by root
Glan culture fluid continues cultivate, after 4-6d, treat that Brassica Napus Seedling length, to 2 true leaves, under condition of salt stress, carries out following 10 kinds
Process: comparison, 0.75% sodium chloride, 0.75% sodium chloride+30umol/L melatonin, 0.75% sodium chloride+45umol/L take off black
Element, 0.75% sodium chloride+60umol/L melatonin, 0.75% sodium chloride+75umol/L melatonin, 0.75% sodium chloride+
0.1umol/L brassinosteroid, 0.75% sodium chloride+0.2umol/L brassinosteroid, 0.75% sodium chloride+0.3umol/L oil
Rape lactone, 0.75%NaCl+0.4umol/L brassinosteroid, often process 7-10 strain, repeat for 2-4 time, and 2-4d changes one
Secondary treatment fluid;
B, the mensuration of physical signs: after processing 8-10d, measure the plant height of 7-10 strain rapeseed plants, leaf area, overground part respectively
Dividing and under ground portion fresh weight, subsequently in the 105-107 DEG C of 30-45min that completes, 80-85 DEG C of drying claims its dry weight to constant weight;
C, data process: each process is carried out variation analysis, according to the root length of rapeseed plants, stem length, aerial parts dry weight and fresh weight of plant seedlings and
Under ground portion dry weight and fresh weight of plant seedlings filters out concentration 30umol/L of melatonin and concentration 0.2umol/L of brassinosteroid respectively;
The application of the compound formulation of d, melatonin and brassinosteroid: under condition of salt stress, arranges following 4 kinds of process: comparison,
0.75% sodium chloride, 0.75% sodium chloride+30umol/L melatonin, 0.75% sodium chloride+0.2umol/L brassinosteroid,
0.75% sodium chloride+30umol/L melatonin+0.2umol/L brassinosteroid, finds 30umol/L melatonin and 0.2umol/L
Brassica campestris L salt damage has been coerced alleviation by the compound adjusting control agent of brassinosteroid, promotes the growth of rapeseed plants.
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Cited By (6)
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CN107347605A (en) * | 2017-08-03 | 2017-11-17 | 中国农业科学院油料作物研究所 | A kind of application of epiphysin in peanut salt damage stress is alleviated |
CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
CN111574289A (en) * | 2020-07-01 | 2020-08-25 | 南京彩韵农林科技有限公司 | Special foliar fertilizer for saline-alkali soil and use method thereof |
CN114868544A (en) * | 2022-04-15 | 2022-08-09 | 江汉大学 | Method for relieving double stress poison of waterlogging and salt of plant seedlings |
CN116548437A (en) * | 2023-03-30 | 2023-08-08 | 中国热带农业科学院橡胶研究所 | Environment-friendly preparation for preventing and treating anthracnose of rubber tree, preparation method and application thereof |
CN117296595A (en) * | 2023-10-25 | 2023-12-29 | 玉林师范学院 | Application of 2,4-epibrassinolide in promoting growth of Yulan in salt stress and method for relieving Yulan salt stress toxicity |
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CN114868544A (en) * | 2022-04-15 | 2022-08-09 | 江汉大学 | Method for relieving double stress poison of waterlogging and salt of plant seedlings |
CN116548437A (en) * | 2023-03-30 | 2023-08-08 | 中国热带农业科学院橡胶研究所 | Environment-friendly preparation for preventing and treating anthracnose of rubber tree, preparation method and application thereof |
CN116548437B (en) * | 2023-03-30 | 2024-02-20 | 中国热带农业科学院橡胶研究所 | Environment-friendly preparation for preventing and treating anthracnose of rubber tree, preparation method and application thereof |
CN117296595A (en) * | 2023-10-25 | 2023-12-29 | 玉林师范学院 | Application of 2,4-epibrassinolide in promoting growth of Yulan in salt stress and method for relieving Yulan salt stress toxicity |
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