CN104255119B - Different Nutrient Elements of feeding is improving the application method in salt tolerance of corn - Google Patents

Different Nutrient Elements of feeding is improving the application method in salt tolerance of corn Download PDF

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CN104255119B
CN104255119B CN201410511838.7A CN201410511838A CN104255119B CN 104255119 B CN104255119 B CN 104255119B CN 201410511838 A CN201410511838 A CN 201410511838A CN 104255119 B CN104255119 B CN 104255119B
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corn
nacl
cacl
kcl
mgcl
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CN104255119A (en
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彭云玲
赵小强
石菁
闫慧萍
武博洋
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ANHUI YINGNUO MANAGEMENT CONSULTING Co.,Ltd.
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Gansu Agricultural University
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Abstract

Different Nutrient Elements of feeding is improving an application method for salt tolerance of corn, key step: based on normal Yoshida nutrient solution, be made into containing different K +, Ca 2+, Mg 2+concentration, but 7 kinds of mixed treating liquids and two the control treatment liquid of determining salinity, carry out paper bed germination test and the research of nutritive cube pot experiment with these 9 kinds for the treatment of fluids and to feed the performance of Different Nutrient Elements at salt tolerance of corn.Result of study shows, Ca 2+maximum to the alleviation of corn, be secondly Mg 2+, K +impact minimum, in germination period and the seedling stage of corn, with 180? mmolL -1naCl+40mgL -1?kCl+60mgL -1?caCl 2+ 40mgL -1?mgCl 2treatment fluid remission effect best.Invention significantly improves the salt resistance of corn germplasm, scientific basis can be provided for salinization soil area corn planting and the regulation and control corn rational application of fertilizer, there is very high application potential.

Description

Different Nutrient Elements of feeding is improving the application method in salt tolerance of corn
Technical field
The invention belongs to agrotechnique application, be specifically related to a kind of Different Nutrient Elements of feeding at the application method improving salt tolerance of corn.
Background technology
The soil salinization is the global problem affecting agricultural production and ecotope.According to statistics, the whole world is subject to the anthropogenic soil of salt stress more than 30%.Excessive salinity not only can cause osmotic stress to plant, interference nutrient ions balance, but also can suppress and induce multiple enzyme system to affect the metabolism of plant, therefore the soil salinization has become one of important envirment factor of improving further of restriction crop yield.
Corn ( zeamaysL.) to salinity medium sensitivity, salt resistance is relatively poor, the existence of a small amount of sodium has certain meaning to its growth, excessively will produce toxic action.The injury of salt stress to corn is mainly manifested in salt ion, particularly Na +can accumulate in a large number in cell, cause Ion toxicity and ion imbalance, and then affect K +and Ca 2+born of the same parents' intensive amount and distribution, the normal growth thus having influence on corn is grown, and finally causes the corn significantly underproduction.
At present salt stress is mainly tended to each several part relevant ions (K in plant corpus to the research of crop ion concentration under salt stress +, Ca 2+, Mg 2+) impact of content, the change of physical signs and different parts ion concentration difference, seldom report as the impact of relevant ions concentration on plant salt endurance in growing environment under condition of salt stress, especially Na in growing environment +, K +, Ca 2+and Mg 2+element acting in conjunction is on the impact of different genotype salt tolerance of corn and the rare bibliographical information of the change of physiological and biochemical index.
In recent years, because people only focus on the contribution of fertilizers input amount to crop yield, and have ignored the harm that environment is brought of excessively applying fertilizer, so cause soil secondary salinization degree very serious, and in the trend increased year by year; Therefore how to improve the salt resistance of crop, the control of salinization soil and comprehensive exploitation are the key subjects that the future of agriculture will solve.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Different Nutrient Elements of feeding at the application method improving salt tolerance of corn.This method by Different Nutrient Elements of feeding, inquires into corn to salt stress (180mmolL in germination period and seedling stage -1naCl) form, Physiology and biochemistry respond, and the cumulative change of each nutritive element in Corn, to disclose Different Nutrient Elements in the inward nature of improving salt tolerance of corn, for the Recent Progress in Study on Salt Tolerance improving crop provides theoretical foundation, for the regulation and control corn rational application of fertilizer provides Technical Reference, for salinization soil area corn planting provides scientific theory foundation.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme: a kind of Different Nutrient Elements of feeding, at the application method improving salt tolerance of corn, comprises the steps:
(1) collection material: collect the Seed of maize inbred that 3 parts of salt resistances are different;
(2) preparation 9 kinds for the treatment of fluids are prepared:
A, redistilled water ddH 2o, blank liquid;
B, 180mmolL -1naCl, salt stress contrast liquid;
C、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2;
D、180mmol·L -1NaCl+60mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
E、180mmol·L -1NaCl+20mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
F、180mmol·L -1NaCl+40mg·L -1KCl+60mg·L -1CaCl 2+40mg·L -1MgCl 2；
G、180mmol·L -1NaCl+40mg·L -1KCl+20mg·L -1CaCl 2+40mg·L -1MgCl 2；
H、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+60mg·L -1MgCl 2；
I、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+20mg·L -1MgCl 2；
Then these 9 kinds for the treatment of fluids are carried out sterilizing at high temperature under high pressure;
Wherein: treatment fluid C is based on normal Yoshida nutrient solution, then to be mixed with salinity be 180mmol.L -1treatment fluid, normal Yoshida nutrient solution comprises 40mgL -1kCl, 40mgL -1caCl 2and 40mgL -1mgCl 2; Treatment fluid D ~ I is based on normal Yoshida nutrient solution, by K +, Ca 2+, Mg 2+concentration improves respectively or reduces 20mgL -1, obtain 20mgL respectively separately -1and 60mgL -1k +, Ca 2+, Mg 2+after the nutrient solution of concentration, then to be made into salinity be 180mmolL -1treatment fluid;
(3) seed cleaning and sterilization: 3 parts of Seed of maize inbreds that selected full consistent, unabroken step (1) obtains, is the hypochlorite disinfectant 10min of 0.5% by percent by volume, then uses ddH 2o rinses 3 ~ 5 times, blots attached water, obtain 3 parts of corn inbred line disinfection seeds with sterilizing filter paper;
(4) soak seed: the disinfection seed of step (3) gained is immersed in respectively 12h, wherein ddH in each treatment fluid of step (2) gained 2o is blank, 180mmolL -1naCl is salt stress contrast;
(5) sprouting test: prepare 3 × 9 × 3 germination boxes, the length × wide × height of germination box is 50cm × 40cm × 30cm; By the hypochlorite disinfectant 10min that germination box percent by volume is 0.5%, use ddH 2o rinses 3 ~ 5 times, and germination box is laid sterilizing double-layer filter paper and made germinating bed, the seed of step (4) gained is placed in germination box respectively, often germination box 30 seeds, repeats for 3 times; Every 1d changes the treatment fluid of corresponding concentration to keep concentration constant, is placed in light culture at phytotron 25 DEG C, adds up percentage of seedgermination, plumule is long, radicle is grown after 7d, plumule fresh weight and radicle fresh weight;
(6) growth of seedling test: after 9 kinds for the treatment of fluids of step (2) gained are mixed by the ratio uniform of 1mL:5g with sterilizing vermiculite respectively, be sub-packed in the nutritive cube of 15cm × 13cm, again the seed of step (4) gained is directly seeded in the sterilizing vermiculite containing same concentrations treatment fluid, 30, every alms bowl, often processes and repeats for 3 times; After planting cultivate in phytotron, growth room daytime/night temperature be 25 ± 2 DEG C/20 ± 2 DEG C, illumination every day 12h, intensity of illumination is 600 μm of ol/ (sm 2), relative moisture 60% ~ 80%, each nutritive cube waters the treatment fluid of same concentrations at regular time and quantity; Seedling grows in the vermiculite being added with different disposal liquid, until blank leaf growth to three leaves wholeheartedly time, carry out the mensuration of every growth indexes and physiological and biochemical index;
(7) determination of ion content: the seedling of 3 parts of inbred lines of gained in step (5) and (6) is used ddH respectively successively 2o rinses well, puts 10min in 105 DEG C of baking ovens, then at 80 DEG C, dries 48h, then measure Na with Plasma-Mass Spectroscopy +, K +, Ca 2+and Mg 2+the content of ion;
(8) interpretation of result: variance analysis and descriptive statistics are carried out to the relative program of the data statistic software SPSS 16.0 recorded in step (5), (6) and (7), result of the test mean value ± standard deviation (SD) represents, by analyzing the performance of Different Nutrient Elements to 3 parts of corn inbred line salt resistances of feeding two periods, and the allotment ratio of Different Nutrient Elements in Corn, disclose K +, Ca 2+and Mg 2+three kinds of nutritive elements, in the inward nature of improving salt tolerance of corn, provide scientific basis for regulating and controlling the corn rational application of fertilizer and making full use of salinization land.
The present invention with the different 3 parts of corn inbred lines of salt resistance for examination material, corn germination period and have studied the K that feeds seedling stage +, Ca 2+and Mg 2+deng three kinds of nutritive elements, pass through 180mmolL -1the performance of salt tolerance of corn after the process of NaCl salt stress, and Na +, K +, Ca 2+and Mg 2+the accumulation of plasma in Corn discloses Different Nutrient Elements in the inward nature of improving salt tolerance of corn, the change of research corn inbred line in form, physio-biochemical characteristics, ion concentration etc., overall scientific ground discloses Different Nutrient Elements in the essence improving salt tolerance of corn, for the regulation and control corn rational application of fertilizer and salinization soil area corn planting provide scientific basis.The method that the present invention passes through is carried out in indoor, simple and easy to do, reproducible, reliable results, Different Nutrient Elements can not only be disclosed in the reason improving salt tolerance of corn from inward nature, but also provide Technical Reference for the Appropriate application of salinization land, improving the salt resistance field of corn and making full use of in salinization land there is very high application potential.
The inventive method shows at the germination period of corn and the K that feeds seedling stage +, Ca 2+and Mg 2+three kinds of nutritive elements, and by salt stress (180mmolL -1naClNaCl) process, the salt resistance of corn germplasm can be significantly improved.
It will be apparent to those skilled in that, Seed of maize inbred test material is not limited to 3 parts, and the present invention, for 3 parts of Seed of maize inbred test materials, is the content in order to clear description technical scheme.
Embodiment
Method therefor in the following embodiment of the present invention, if no special instructions, is conventional method; Equipment used, reagent are conventional equipment, the reagent that commercial sources is bought from Reagent Company.
Different Nutrient Elements of feeding is improving the application method of salt tolerance of corn, carries out according to the following steps:
1. collection material: collect salt-tolerant inbred lines 8723,81162 and salt density value inbred line P138 tri-parts of kind matter.
2. prepare treatment fluid:
A, redistilled water ddH 2o, blank liquid;
B, 180mmolL -1naCl, salt stress contrast liquid;
C、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2;
D、180mmol·L -1NaCl+60mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
E、180mmol·L -1NaCl+20mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
F、180mmol·L -1NaCl+40mg·L -1KCl+60mg·L -1CaCl 2+40mg·L -1MgCl 2；
G、180mmol·L -1NaCl+40mg·L -1KCl+20mg·L -1CaCl 2+40mg·L -1MgCl 2；
H、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+60mg·L -1MgCl 2；
I、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+20mg·L -1MgCl 2；
Then these 9 kinds for the treatment of fluids are carried out sterilizing at high temperature under high pressure.Wherein treatment fluid C is based on normal Yoshida nutrient solution, then to be mixed with salinity be 180mmol.L -1treatment fluid, normal Yoshida nutrient solution comprises 40mgL -1kCl, 40mgL -1caCl 2and 40mgL -1mgCl 2; Treatment fluid D ~ I is based on normal Yoshida nutrient solution, by K +, Ca 2+, Mg 2+concentration improves respectively or reduces 20mgL -1, obtain 20mgL respectively separately -1and 60mgL -1k +, Ca 2+, Mg 2+after nutrient solution, then to be made into salinity be 180mmolL -1the treatment fluid of NaCl.
3. seed disinfection: select full consistent, unabroken seed from 3 parts of corn germplasms, after percent by volume 0.5% hypochlorite disinfectant 10min, then uses ddH 2o rinses 3 ~ 5 times, blots attached water, obtain 3 parts of corn inbred line disinfection seeds with sterilizing filter paper.
4. soak seed: the disinfection seed of step 3 gained is immersed in respectively 12h in each treatment fluid of step 2 gained, for subsequent use.
5. sprouting test: prepare 3 × 9 × 3 germination boxes, the length × wide × height of germination box is 50cm × 40cm × 30cm; By the hypochlorite disinfectant 10min that germination box percent by volume is 0.5%, use ddH 2o rinses 3 ~ 5 times, and germination box is laid sterilizing double-layer filter paper and made germinating bed, and after step 4 being soaked seed, the seed of gained is placed in germination box respectively, often germination box 30 seeds, repeats for 3 times; Every 1d changes the treatment fluid of corresponding concentration to keep concentration constant, is placed in light culture at phytotron 25 DEG C, adds up percentage of seedgermination, plumule is long, radicle is long after 7d, plumule fresh weight and radicle fresh weight etc. sprout index.
6. growth of seedling test: after 9 of step 2 gained kinds for the treatment of fluids are mixed by the ratio uniform of 1mL:5g with sterilizing vermiculite respectively, be sub-packed in the nutritive cube of 15cm × 13cm, again step 4 is directly seeded in the sterilizing vermiculite containing same concentrations treatment fluid through the seed of seed soaking, 30, every alms bowl, often processes and repeats for 3 times; After planting cultivate in phytotron, growth room daytime/night temperature be 25 ± 2 DEG C/20 ± 2 DEG C, illumination every day 12h, intensity of illumination is 600 μm of ol/ (sm 2), relative moisture 60% ~ 80%, each nutritive cube waters the treatment fluid of same concentrations at regular time and quantity; Seedling grows in the vermiculite being added with different disposal liquid, until blank leaf growth to three leaves wholeheartedly time, carry out the mensuration of every growth indexes and physiological and biochemical index.
7. determination of ion content: the seedling of 3 parts of inbred lines of step 5 germination period and step 6 gained in seedling stage is used ddH respectively successively 2o rinses well, puts 10min in 105 DEG C of baking ovens, then at 80 DEG C, dries 48h, then measure Na with Plasma-Mass Spectroscopy +, K +, Ca 2+and Mg 2+the content of ion.
8. experimental result:
8.1 germination periods are fed the performance of Different Nutrient Elements to salt tolerance of corn
As shown in Table 1, in the corn germinal phase, after Applied potassium of feeding, calcium, magnesium, corn inbred line upgrowth situation comparatively only has ddH 2the upgrowth situation of O blank is poor, but more only feeds 180mmolL -1the upgrowth situation of NaCl salt stress contrast will be got well, illustrate that Applied potassium, calcium, magnesium still have certain mitigation to salt stress, effectively can improve the salt resistance of corn, but these three kinds of nutritive elements of potassium, calcium, magnesium are different to the influence degree of germination period seed growth situation, Ca 2+maximum to the alleviation of Course of Corn Seed Germination, be secondly Mg 2+, K +impact minimum, and as can be seen from the index such as plumule length, radicle length, plumule fresh weight, radicle fresh weight, F process, i.e. 180mmolL -1naCl+40mgL -1kCl+60mgL -1caCl 2+ 40mgL -1mgCl 2the remission effect of solution-treated is best.
Note: in table, A-I represents 9 kinds of different disposal containing A-I9 kind treatment fluid respectively.Lower same.
As shown in Table 2, the upgrowth situation of salt-tolerant inbred lines will significantly better than salt density value inbred line.Salt-tolerant inbred lines 8723 is with 81162 compared with salt density value inbred line P138, and plumule is long, and radicle is long, plumule fresh weight, and radicle fresh weight increases 11.55%, 14.67%, 10.71%, 12.20% and 17.73%, 11.05%, 17.86% respectively, and 26.83%.
Note: same index indicates different lowercase or capitalization person between different inbred line measured value is significant difference under 5% or 1% level.Lower same.
The performance of Different Nutrient Elements of feeding for 8.2 seedling stages to salt tolerance of corn
Shown in table 3 and table 4, in seedling growth stage, corn inbred line upgrowth situation comparatively ddH after Applied potassium of feeding, calcium, magnesium 2the upgrowth situation of O blank is poor, but comparatively 180mmolL -1the upgrowth situation of NaCl salt stress contrast will be got well, and illustrates that the growth to salt stress Maize Seedling inbred line of Applied potassium, calcium, magnesium has certain mitigation, but these three kinds of nutritive elements are different to the influence degree of seedling growth, Ca 2+maximum to the alleviation of Course of Corn Seed Germination, be secondly Mg 2+, K +impact minimum, and in plant height, root length, overground part fresh weight, underground part fresh weight, relative conductivity, mda content, proline content, soluble sugar content, SOD are active, CAT is active, POD is active etc., and index can be found out, F process, i.e. 180mmolL -1naCl+40mgL -1kCl+60mgL -1caCl 2+ 40mgL -1mgCl 2the remission effect of solution-treated is best.
Shown in table 5 and table 6, the upgrowth situation of salt-tolerant inbred lines will significantly better than salt density value inbred line.Salt-tolerant inbred lines 8723 with 81162 compared with salt density value inbred line P138, plant height, root length, overground part fresh weight, underground part fresh weight, relative water content, proline content, soluble sugar content, SOD are active, POD is active, CAT is active increases 25.69%, 29.97%, 67.98%, 30.00%, 8.14%, 33.21%, 5.66%, 16.28%, 70.34%, 43.86% respectively, and relative conductivity, mda content reduce 6.05%, 11.44% respectively.
The accumulation of 8.3 Different Nutrient Elements in corn seedling body
Shown in table 7 and table 8, generally speaking, after Applied potassium of feeding, calcium, magnesium, K in germination period and Maize Seedling plant body +, Ca 2+, Mg 2+content is ddH all comparatively 2o blank and 180mmolL -1the content of NaCl salt stress contrast is high, and Na +content, Na +/ K +, Na +/ Ca 2+, Na +/ Mg 2+ratio is between therebetween.Illustrate that Applied potassium, calcium, magnesium have certain mitigation to salt stress, but these three kinds of nutritive elements are different to the influence degree of seedling growth.
Shown in table 9 and table 10, after feeding Applied potassium, calcium, magnesium process two periods, the K of salt-tolerant inbred lines plant +, Ca 2+, Mg 2+content and Na +/ K +, Na +/ Ca 2+, Na +/ Mg 2+ratio all pole is significantly higher than salt density value inbred line, and Na +content is extremely remarkable in salt density value inbred line.
As can be seen from above-mentioned example, the inventive method in corn two growthdevelopmental stages and germination period and seedling stage, by the K that feeds +, Ca 2+, Mg 2+after three kinds of nutritive elements, have studied Different Nutrient Elements to the form of corn salt stress, Physiology and biochemistry response and the accumulation of Different Nutrient Elements in Corn, science, objective, disclose Different Nutrient Elements of feeding improving the essence of salt tolerance of corn all sidedly, demonstrate K +, Ca 2+, Mg 2+etc. the salt resistance that can improve corn significantly, for salinization soil area corn planting and scientific and reasonable regulation and control corn fertilizing lay a solid foundation, there is very high using value.
By research germination period and the performance of Different Nutrient Elements to 3 parts of corn inbred line salt resistances of feeding seedling stage, and Na +, K +, Ca 2+and Mg 2+show Deng after the allotment ratio of nutritive element in corn seedling body, corn this two period Ca 2+maximum to the mitigation of corn, be secondly Mg 2+, K +impact minimum, and as can be seen from the index such as growth indexes and the growth in seedling stage, Physiology and biochemistry of germination period, F process, i.e. 180mmolL -1naCl+40mgL -1kCl+60mgL -1caCl 2+ 40mgL -1mgCl 2the remission effect of solution-treated is best.This result of study provides scientific basis for improving Recent Progress in Study on Salt Tolerance of Corn, for salinization soil area corn planting and the regulation and control corn rational application of fertilizer are taken a firm foundation, has very high application potential.

Claims (1)

1. Different Nutrient Elements of feeding, improving the application method in salt tolerance of corn, comprises the steps:
(1) collection material: collect the Seed of maize inbred that 3 parts of salt resistances are different;
(2) preparation 9 kinds for the treatment of fluids are prepared:
A, redistilled water ddH 2o, blank liquid;
B, 180mmolL -1naCl, salt stress contrast liquid;
C、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2;
D、180mmol·L -1NaCl+60mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
E、180mmol·L -1NaCl+20mg·L -1KCl+40mg·L -1CaCl 2+40mg·L -1MgCl 2；
F、180mmol·L -1NaCl+40mg·L -1KCl+60mg·L -1CaCl 2+40mg·L -1MgCl 2；
G、180mmol·L -1NaCl+40mg·L -1KCl+20mg·L -1CaCl 2+40mg·L -1MgCl 2；
H、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+60mg·L -1MgCl 2；
I、180mmol·L -1NaCl+40mg·L -1KCl+40mg·L -1CaCl 2+20mg·L -1MgCl 2；
Then these 9 kinds for the treatment of fluids are carried out sterilizing at high temperature under high pressure;
Wherein: treatment fluid C is based on normal Yoshida nutrient solution, then to be mixed with salinity be 180mmol.L -1treatment fluid, normal Yoshida nutrient solution comprises 40mgL -1kCl, 40mgL -1caCl 2and 40mgL -1mgCl 2; Treatment fluid D ~ I is based on normal Yoshida nutrient solution, by K +, Ca 2+, Mg 2+concentration improves respectively or reduces 20mgL -1, obtain 20mgL respectively separately -1and 60mgL -1k +, Ca 2+, Mg 2+after nutrient solution, then to be made into salinity be 180mmolL -1the treatment fluid of NaCl;
(3) seed cleaning and sterilization: 3 parts of Seed of maize inbreds that selected full consistent, unabroken step (1) obtains, is the hypochlorite disinfectant 10min of 0.5% by percent by volume, then uses ddH 2o rinses 3 ~ 5 times, blots attached water, obtain 3 parts of corn inbred line disinfection seeds with sterilizing filter paper;
(4) soak seed: the disinfection seed of step (3) gained is immersed in respectively 12h, wherein ddH in each treatment fluid of step (2) gained 2o is blank, 180mmolL -1naCl is salt stress contrast;
(5) sprouting test: prepare 3 × 9 × 3 germination boxes, the length × wide × height of germination box is 50cm × 40cm × 30cm; By the hypochlorite disinfectant 10min that germination box percent by volume is 0.5%, use ddH 2o rinses 3 ~ 5 times, and germination box is laid sterilizing double-layer filter paper and made germinating bed, the seed of step (4) gained is placed in germination box respectively, often germination box 30 seeds, repeats for 3 times; Every 1d changes the treatment fluid of corresponding concentration to keep concentration constant, is placed in light culture at phytotron 25 DEG C, adds up percentage of seedgermination, plumule is long, radicle is grown after 7d, plumule fresh weight and radicle fresh weight;
(6) growth of seedling test: after 9 kinds for the treatment of fluids of step (2) gained are mixed by the ratio uniform of 1mL:5g with sterilizing vermiculite respectively, be sub-packed in the nutritive cube of 15cm × 13cm, again the seed of step (4) gained is directly seeded in the sterilizing vermiculite containing same concentrations treatment fluid, 30, every alms bowl, often processes and repeats for 3 times; After planting cultivate in phytotron, growth room daytime/night temperature be 25 ± 2 DEG C/20 ± 2 DEG C, illumination every day 12h, intensity of illumination is 600 μm of ol/ (sm 2), relative moisture 60% ~ 80%, each nutritive cube waters the treatment fluid of same concentrations at regular time and quantity; Seedling grows in the vermiculite being added with different disposal liquid, until blank leaf growth to three leaves wholeheartedly time, carry out the mensuration of every growth indexes and physiological and biochemical index;
(7) determination of ion content: the seedling of 3 parts of inbred lines of gained in step (5) and (6) is used ddH respectively successively 2o rinses well, puts 10min in 105 DEG C of baking ovens, then at 80 DEG C, dries 48h, then measure Na with Plasma-Mass Spectroscopy +, K +, Ca 2+and Mg 2+the content of ion;
(8) interpretation of result: variance analysis and descriptive statistics are carried out to the relative program of the data statistic software SPSS 16.0 recorded in step (5), (6) and (7), result of the test mean value ± standard deviation (SD) represents, by analyzing the performance of Different Nutrient Elements to 3 parts of corn inbred line salt resistances of feeding two periods, and the allotment ratio of Different Nutrient Elements in Corn, disclose K +, Ca 2+and Mg 2+three kinds of nutritive elements are in the inward nature of improving salt tolerance of corn.
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CN103718698B (en) * 2014-01-07 2015-05-06 江苏省农业科学院 Method for distinguishing salt tolerance of wheat in germination period
CN103828713A (en) * 2014-02-25 2014-06-04 江苏沿海地区农业科学研究所 Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley
CN104012267A (en) * 2014-04-30 2014-09-03 中国农业科学院作物科学研究所 Maize salt tolerance identification method

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