CN109187863A - A kind of greening seedling Seedling Salt-tolerance identification method - Google Patents
A kind of greening seedling Seedling Salt-tolerance identification method Download PDFInfo
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- CN109187863A CN109187863A CN201811020545.3A CN201811020545A CN109187863A CN 109187863 A CN109187863 A CN 109187863A CN 201811020545 A CN201811020545 A CN 201811020545A CN 109187863 A CN109187863 A CN 109187863A
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Abstract
The present invention relates to a kind of greening seedling Seedling Salt-tolerance identification methods, the relatively uniform greening seedling seedling of growth and development is selected first, is transplanted in the basin for being filled with extruding vermiculite, then pours Hoagland nutrient solution daily, progress rain cover light transmission maintenance management, slow seedling 1-2 weeks；It is again that the stress salt water that basic liquid adds sodium chloride to prepare various concentration carries out salt stress processing from low concentration to high concentration step by step with Hoagland nutrient solution；After treatment, clip nursery stock aerial part carry out weighing record, carry out regression curve estimation analysis to data using SPSS, obtain response curve formula, after calculating salt stress attenuation coefficient, then calculate the salt-tolerant threshold of the nursery stock.This method solve Distribution of soil salinity present in existing identification method, and uneven, moisture and salt regime controls the problems such as difficult, and qualification result repeatability of the present invention is strong, can accurately determine the practical salt resistance ability of nursery stock, be suitable for the fields such as nursery stock New salt-tolerant cultivar breeding.
Description
Technical field
The invention belongs to ornamental plant technical fields, are related to a kind of greening seedling Seedling Salt-tolerance identification method.
Background technique
Salinized soil is a kind of soil types widely distributed on the earth, serious to hinder local agriculture and forestry development, influences ecology
The improvement of environment becomes one of the key constraints of salty area economic development.Plantation salt-tolerant plant is improvement salinized soil
One of effective way.And planting salt-tolerant plant first needs the salt resistance ability of clear plant, needs to carry out accurate salt tolerant to plant
Identification, Tolerant salt method applied by people is various at present, and standard is different, and there are respective defects.Such as: salt-soda soil
Distribution of soil salinity existing for the identification method of crop field is uneven, satisfactory solonchak is not easy to find problem；Solonchak method for potted
There are moisture and salt regimes to control difficult problem；There are Activities of Some Plants root systems to breathe for water planting Tolerant salt, and root system is caused to be easy corruption
Rotten problem.It differs greatly between above-mentioned each method qualification result, and repeatability is poor, it is difficult to which the accurate reality for determining nursery stock is resistance to
Salt ability.
Summary of the invention
Present invention aim to address the deficiencies of above-mentioned background technique, provide a kind of greening seedling Seedling Salt-tolerance identification side
Method is mainly used in saline land greening engineering, vegetation ecological recovery project and the choosing of nursery stock New salt-tolerant cultivar based on the sodium chloride
Educate equal fields.
The technical solution adopted in the present invention is as follows:
A kind of greening seedling Seedling Salt-tolerance identification method, includes the following steps:
(1) the relatively uniform greening seedling seedling of growth and development is selected, the extruding leech for being filled with that partial size is 1-3mm is transplanted to
In the basin of stone, then daily pour Hoagland nutrient solution, carry out rain cover light transmission maintenance management, slow seedling 1-2 weeks, until there is young leaves
Expansion；
(2) the stress salt water for adding sodium chloride preparation various concentration for basic liquid with Hoagland nutrient solution, to step (1)
After greening seedling slow seedling, from low concentration to high concentration, salt stress processing is carried out step by step；
(3) salt stress after treatment, the aerial part that clip respectively handles nursery stock carries out weighing record, using SPSS logarithm
According to regression curve estimation analysis is carried out, response curve formula I is obtained:
Y=ae-bx (I)
Wherein, y- plant above ground fresh weight, unit g；A-CK measured value, unit g；The bottom of e- natural logrithm；The b- salt side of body
Compel attenuation coefficient；
With P50Indicate salinity when biomass reduces half, i.e., the salt-tolerant threshold of identified greening seedling, through taking
Logarithm letter
Calculation formula II after change is as follows:
P50=0.6931/b+x0 (II)
Wherein, b- salt stress attenuation coefficient；x0The salinity of biomass maximum processing；
The salt-tolerant threshold P of the nursery stock is calculated according to formula I and II50。
In step (1), the greening seedling is selected from red-spotted stonecrop, willow, poplar, elm, Mains micromalus, Chinese tamarisk, birch-leaf pear, gold and silver
Any one in flower, lotus certain herbaceous plants with big flowers, chrysanthemum, rose, Chinese rose, Fructus Forsythiae, leaflet winter creeper, Tartar's honeysuckle or false indigo.
In step (1), the height of the basin is 30-50cm, and basin mouth internal diameter is 30-50cm, and every basin uniformly transplants 4 plants of greenings
Nursery stock seedling.
In step (2), the salt stress processing method particularly includes: first day, first use the stress salt water of minimum concentration
Excessive pouring carried out to nursery stock, second day, excessive pouring is carried out to nursery stock using the stress salt water of the second low concentration, third day,
Excessive pouring is carried out to nursery stock using the stress salt water of the second low concentration, and so on, until the maximum concentration using setting
After coercing the excessive pouring of salt water progress, continue stress salt water irrigation 55-60 days using the concentration, salt stress terminates.Due to leech
Stone water-holding capacity is larger, is irrigated until must having salt water to flow out from flowerpot bottom every time.
The utility model has the advantages that the present invention does matrix using vermiculite, seedling stage Salt Strees Condition is carried out with heavy irrigation saline methods, with seedling
The wooden ground fresh weight is salt-tolerant threshold parameter, evaluates the salt tolerance of greening seedling, solves salt-soda soil crop field identification method and deposit
Distribution of soil salinity is uneven, satisfactory solonchak is not easy to find problem, also solving solonchak method for potted, there are water
Salt move control difficult problem, also solving water planting Tolerant salt, there are Activities of Some Plants root systems to breathe, and root system is caused to be easy
Rotten problem；Qualification result repeatability of the present invention is strong, can accurately determine the practical salt resistance ability of nursery stock, and can realize not
With the lateral comparison of salt resistance ability between nursery stock, qualification result may be directly applied to saline land greening engineering based on the sodium chloride,
The fields such as vegetation ecological recovery project and nursery stock New salt-tolerant cultivar breeding.
Detailed description of the invention
Fig. 1 is the salt stress regression curve of the red-spotted stonecrop of three kinds in embodiment 1.
Specific embodiment
The following describes the present invention in detail with reference to the accompanying drawings and specific embodiments.
In following embodiments, for greening seedling red-spotted stonecrop, Salt-Tolerance Identification is carried out, but it is not limited to this.
Embodiment 1
By safflower, eight treasures (choice ingredients of certain special dishes), three kinds of long medicine red-spotted stonecrop Salt-Tolerance Identification for, illustrate that the method for the present invention completely walks
Suddenly.
A kind of greening seedling Seedling Salt-tolerance identification method, includes the following steps:
(1) the relatively uniform greening seedling seedling of growth and development is selected, the extruding leech for being filled with that partial size is 1-2mm is transplanted to
(every potting plants 4 plants, is arranged 6 processing, 4 basins of each processing, totally 24 basin), then daily pouring Hoagland nutrition in the basin of stone
Liquid, carries out daily maintenance management, and slow seedling 1-2 weeks, until there is young leaves expansion；
It (2) is that the stress salt water that basic liquid adds sodium chloride to prepare various concentration (it is resistance to search red-spotted stonecrop with Hoagland nutrient solution
Salt related data, it is contemplated that red-spotted stonecrop threshold value of surviving is 0.8, the stress brine strength for setting 6 various concentrations is respectively as follows: 0,
0.2%, 0.4%, 0.6%, 0.8%, 1.0%), after the greening seedling slow seedling of step (1), from low concentration to high concentration, by
Grade carries out salt stress processing；
Salt stress processing method particularly includes: i.e. Hoagland nutrient solution is control to the stress salt water for being 0 using concentration, and first
It, in addition to Hoagland nutrient solution is poured in control, the NaCl solution of remaining processing pouring 0.1%, second day, except control is poured
Outside Hoagland nutrient solution, 0.2% NaCl solution, third day, except control and 0.2% processing (are poured are poured in remaining processing
0.2%) outside, 0.3% NaCl solution is poured in remaining processing, and so on, the tenth day, each processing respectively reach CK, 0.2%,
0.4%, 0.6%, 0.8%, 1.0%, it is excessive daily to pour containing setting NaCl concentration after each authentication process reaches setting concentration
Nutrient solution, this section salt stress handling duration 60 days, until red-spotted stonecrop salt damage symptom is in 5 days without substantially changeing.
(3) salt stress after treatment, the aerial part that clip respectively handles nursery stock carries out weighing record, using SPSS logarithm
According to regression curve estimation analysis (curve graph is shown in Fig. 1) is carried out, three kind response curve formula I are obtained:
Eight treasures (choice ingredients of certain special dishes) formula I:y=110.606e-1.565x
Safflower formula I:y=128.884e-1.982x
Long medicine formula I:y=91.785e-1.696x
With P50Indicate salinity when biomass reduces half, i.e., the salt-tolerant threshold of identified plant.Through taking logarithm
Simplified calculation formula (2) is as follows:
P50=0.6931/b+x0
The salt-tolerant threshold P of the nursery stock is calculated according to formula I and II50Namely nursery stock biomass is reduced to control biology
Suffered salt stress concentration when half is measured, the results are shown in Table 1:
Table 1
According to the test result of table 1, it may infer that safflower red-spotted stonecrop is no more than 0.349 in the total salt content based on sodium chloride
Saline-alkali soil on can transplant, and have preferable afforestation effect；Eight treasures (choice ingredients of certain special dishes) red-spotted stonecrop does not surpass in the total salt content based on sodium chloride
Crossing on 0.443 saline-alkali soil can transplant, and have preferable afforestation effect；Sedum spectabile contains in the total salt based on sodium chloride
Amount can transplant on the saline-alkali soil no more than 0.409, and have preferable afforestation effect.
Claims (4)
1. a kind of greening seedling Seedling Salt-tolerance identification method, which comprises the steps of:
(1) the relatively uniform greening seedling seedling of growth and development is selected, the extruding vermiculite for being filled with that partial size is 1-3mm is transplanted to
In basin, then daily pour Hoagland nutrient solution, carry out rain cover light transmission maintenance management, slow seedling 1-2 weeks, until there is young leaves exhibition
It opens；
(2) add the stress salt water of sodium chloride preparation various concentration, the greening to step (1) for basic liquid with Hoagland nutrient solution
After nursery stock slow seedling, from low concentration to high concentration, salt stress processing is carried out step by step；
(3) salt stress after treatment, the aerial part that clip respectively handles nursery stock carry out weighing record, using SPSS to data into
The estimation analysis of row regression curve, obtains response curve formula I:
Y=ae-bx (I)
Wherein, y- plant above ground fresh weight, unit g；A-CK measured value, unit g；The bottom of e- natural logrithm；B- salt stress declines
Subtract coefficient；
With P50Indicate salinity when biomass reduces half, i.e., the salt-tolerant threshold of identified greening seedling, through taking logarithm
Simplified calculation formula II is as follows:
P50=0.6931/b+x0 (II)
Wherein, b- salt stress attenuation coefficient；x0The salinity of biomass maximum processing；
The salt-tolerant threshold P of the nursery stock is calculated according to formula I and II50。
2. greening seedling Seedling Salt-tolerance identification method as described in claim 1, which is characterized in that described green in step (1)
Change nursery stock and is selected from red-spotted stonecrop, willow, poplar, elm, Mains micromalus, Chinese tamarisk, birch-leaf pear, honeysuckle, lotus certain herbaceous plants with big flowers, chrysanthemum, rose, the moon
Any one in season, Fructus Forsythiae, leaflet winter creeper, Tartar's honeysuckle or false indigo.
3. greening seedling Seedling Salt-tolerance identification method as described in claim 1, which is characterized in that in step (1), the basin
Height be 30-50cm, basin mouth internal diameter is 30-50cm, and every basin uniformly transplants 4 plants of greening seedling seedling.
4. the greening seedling Seedling Salt-tolerance identification method as described in claims 1 or 2 or 3, which is characterized in that in step (2),
The salt stress processing method particularly includes: first day, excessive pouring is first carried out to nursery stock using the stress salt water of minimum concentration,
Second day, excessive pouring, third day, using the stress of the second low concentration are carried out to nursery stock using the stress salt water of the second low concentration
Salt water carries out excessive pouring to nursery stock, and so on, until the stress salt water of the maximum concentration using setting carries out excessive pouring
Afterwards, continue stress salt water irrigation 55-60 days using the concentration, salt stress terminates.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110731223A (en) * | 2019-11-15 | 2020-01-31 | 南京林业大学 | Method for promoting growth of salix jinguiensis under stress of salts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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Application publication date: 20190111 |