CN106472137A - A kind of method improving Salt Tolerance in Rice Seedlings - Google Patents
A kind of method improving Salt Tolerance in Rice Seedlings Download PDFInfo
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- CN106472137A CN106472137A CN201610834610.0A CN201610834610A CN106472137A CN 106472137 A CN106472137 A CN 106472137A CN 201610834610 A CN201610834610 A CN 201610834610A CN 106472137 A CN106472137 A CN 106472137A
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- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
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- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention relates to a kind of method improving Salt Tolerance in Rice Seedlings, belong to Rice Salt application.The method is described as：Before rice paddy seed fresh water presoaking and germinating, first use 1.8%NaCl saline solution to soak seed 1 day, then carry out conventional fresh water presoaking and germinating, the salt tolerance of rice seedling can be significantly improved in pole.The present invention is to solving the problems, such as that Coastal beach salt tolerant rice kind is few, existing salt-enduring cultivars yield is not high, disease resistance particularly blast resistance is poor, quality is bad provides fine approach.
Description
First, technical field
The present invention relates to a kind of method improving Salt Tolerance in Rice Seedlings, belong to Rice Salt application.
2nd, background technology
China mainland coastline is up to 1.8 ten thousand kilometers, is distributed in 11 coastal state such as the Liao Dynasty, Ji, Tianjin, Shandong, Soviet Union, Shanghai, Zhejiang, Fujian
(city, autonomous region).According to investigations, 35,180,000 mu of China's existing beach gross area, wherein 10,310,000 mu of Jiangsu, nearly account for 30%, occupy
The hat in the whole nation.7,500,000 mu of the beach do not enclosed and cultivated, is still become silted up with annual 1.9 ten thousand mu of speed at present and grows, be the rare soil of China
Ground reserved resources, have huge potentiality to be exploited and important value.In China, existing cultivated land resource has obtained sufficiently
Excavate, conventional Farmland Productivity level is lifted in the case of facing bottleneck further, strengthen coastal marsh development using to lifting
China's arable land farming production capacity, observes the red line of 1,800,000,000 mu of cultivated areas, ensures national food security, has important reality meaning
Adopted and long-range strategic importance.
Develop Tidal Flat Resources, be human use's nature, the struggle of nature remodeling, be the weight promoting socio-economic development
Want one of approach.Oryza sativa L. is China the first generalized grain crop, the grain ration of China more than 60% population based on rice, rice yield
The big staple food crop of China three comes out at the top.China coast beach is all located in China the Changjiang river, the Yellow River, Huaihe River, the Zhujiang River, black
, there are more sufficient freshwater resources in the estuary region of the trunk rivers such as Longjiang, and the Coastal beach after reclaiming is conducive to rice seed
Plant.Coastal marsh development using during, due to paddy growth during have special water environment, beach earth's surface through water cover can
Acted on the saline and alkaline leaching pressure salt salt reduction that plays of solubility to the soil body, beach earth's surface covers through water and can also play soil body principle of balance fertilizer
The effect of power, along with the biological agent reducing salinity specific to Oryza sativa L. itself, it is organic that rice straw also field is to increase soil
Matter, improvement salt-soda soil, effective measures of fertilizing soil, therefore Oryza sativa L. is the first-selected grain of Coastal beach and alkaline land improving
Crop.
For China coast beach salt tolerant rice kind few, existing salt-enduring cultivars yield is not high, disease resistance particularly
The present situation that blast resistance is poor, quality is bad, screens salt tolerant product from existing high yield, disease-resistant, high-grade rice kind or strain
Plant or improve by technological means research the salt tolerance of existing kind (being), Coastal beach can be met salt tolerant rice kind is compeled
It is essential and ask.
3rd, content of the invention
Technical problem
The object of the invention in order to solve China coast beach to high yield, high-quality, disease-resistant salt tolerant rice kind urgent needss
And provide a kind of method improving Salt Tolerance in Rice Seedlings.
Technical scheme
A kind of improve Salt Tolerance in Rice Seedlings method it is characterised in that Oryza sativa L. through saline seed soaking a period of time after, its Seedling
The salt tolerance of phase increases substantially.
Described saline seed soaking：It is the NaCl saline solution seed soaking with 1.8%.
Described saline seed soaking a period of time：Refer to 1.8% NaCl saline solution soak seed 1 day, after with fresh water continue seed soaking
To showing money or valuables one carries unintentionally.
Described Seedling Salt-tolerance authentication method is：Judge that Seedling Salt-tolerance is in 0.5%NaCl saline with rice seedling
Survival rate after growing 21 days under stress is index.Specifically the seed showing money or valuables one carries unintentionally is moved in the PCR plate fined away in 96 holes, will
PCR plate is placed in the cuboid tank body filling with water.When Seedling grows to 1 leaf 1 heart, the water in tank body is changed into mill water culture nutrient solution.Treat
When Seedling grows to 2 leaf 1 heart, the nutritional solution in tank body is changed into the rice nutrition liquid containing 0.5%NaCl, carry out salt stress process, every 3
It changes the nutritional solution of a saliferous, and the salt treatment time is 21 days, after process terminates, is placed in rehydration in nutritional solution and, after 7 days, investigates
The survival rate of each kind.Described rice nutrition formula of liquid contains:N 40mg/L, P 10mg/L, K 40mg/L, Ca 40mg/L,
Mg 40mg/L, Fe 2mg/L, Mn 0.50mg/L, Mo 0.05mg/L, B 0.20mg/L, Zn 0.01mg/L, Cu 0.01mg/
L, Si 56mg/L (Laboratory manual for physiological studies of rice, Ed 3.The
International Rice Research Institute, 1976).
Beneficial effect
The present invention provides a kind of method improving Salt Tolerance in Rice Seedlings, can solve China coast beach to high yield, excellent
Matter, the urgent needss of disease-resistant salt tolerant rice kind, have greatly saved the Rice Salt breed breeding time, have saved manpower and thing
Power.
The present invention can make Salt Resistance of Rice highest increase by 1.9 times (Fig. 2).
4th, brief description
Fig. 1 difference salinity, different disposal time rice shoot survival rate
The impact to different salt tolerant type Varieties In The Seedling Stage salt tolerances for Fig. 2 high salt concentration water logging kind
The impact to different salt tolerant type kind germination percentages for Fig. 3 high salt concentration water logging kind
5th, specific embodiment
1) determination of salt treatment time and concentration：Test (is authorized numbering Soviet Union and is examined rice from the kind south round-grained rice 46 of moderate salt tolerant
200814), first processed respectively 1 day and 2 days with the saline solution of 0%NaCl, 0.6%NaCl, 1.2%NaCl, 1.8%NaCl, place
Reason continues seed soaking with fresh water, until showing money or valuables one carries unintentionally after terminating.The seed showing money or valuables one carries unintentionally is moved in the PCR plate fined away in 96 holes, 48/product
Kind, 2 repetitions, PCR plate is placed in the cuboid tank body filling with water.When Seedling grows to 1 leaf 1 heart, the water in tank body is changed into
Mill water culture nutrient solution.When Seedling grows to 2 leaf 1 heart, the nutritional solution in tank body is changed into the rice nutrition liquid containing 0.5%NaCl, carries out
Salt stress is processed, the nutritional solution changing a saliferous in every 3 days, and the salt treatment time is 21 days, after process terminates, is placed in nutritional solution
After rehydration 7 days, investigate the survival rate of each kind.Result shows：1.8%NaCl seed soaking continues fresh water seed soaking after 1 day, this
Method can make Salt Tolerance in Rice Seedlings can bring up to 1.9 times (Fig. 1).Described rice nutrition formula of liquid is:N 40mg/L, P
10mg/L, K40mg/L, Ca 40mg/L, Mg 40mg/L, Fe 2mg/L, Mn 0.50mg/L, Mo 0.05mg/L, B 0.20mg/
L, Zn 0.01mg/L, Cu 0.01mg/L, Si 56mg/L (Laboratory manual for physiological
Studies of rice, Ed 3.The International Rice Research Institute, 1976).
2) a kind of checking of the method improving Salt Tolerance in Rice Seedlings:In order to verify the reliability of said method, go forward side by side one
Step research the impact to Salt Tolerance in Rice Seedlings for the more high salt concentration, selected strong salt tolerant (peaceful round-grained rice 4 (state examines rice 2009040)),
Moderate salt tolerant (Japan fine (national crops preserving seed center, numbering：WD-10576；Agricultural University Of Jiangxi's journal, 2015,37
(5)：781-787))) and salt density value (prick Sigma (Jiangsu Province agricultural germ plasm resource mid-term storehouse, numbering M3A00405468；North China agriculture
Journal, 2013,28 (1)：58-61)) rice varieties of 3 kinds of different salt tolerant types are tested, use respectively 0%NaCl, 1.8%
NaCl, 2.2%NaCl saline solution continues fresh water after soaking seed 1 day and soaks seed to showing money or valuables one carries unintentionally.Seedling Salt-tolerance is identified ibid.Experimental result table
Bright：The kind of 3 kinds of different salt tolerant types equally can significantly improve rice seedling salt tolerant in pole through 1.8%NaCl seed soaking after 1 day
Property, peaceful No. 4 salt tolerances of round-grained rice improve 87.9%, Japanese fine salt tolerance and improve 39.4%, prick Sigma's salt tolerance and improve
90.3% (Fig. 2).Meanwhile, salt treatment promotes the raising of percentage of seedgermination, and peaceful No. 4 germination percentages of round-grained rice improve -0.9%, and Japan is fine
Germination percentage improves 4.4%, pricks Sigma's germination percentage and improves 1.9% (Fig. 3).
Test, from the high yield of spread, high-quality, disease-resistant rice varieties (being), first uses the salt of 1.8%NaCl molten
Immersion kind 1 day, continues seed soaking with fresh water after terminating, until showing money or valuables one carries unintentionally.The seed showing money or valuables one carries unintentionally is moved in the PCR plate fined away in 96 holes,
48/kind (being), 2 repetitions, PCR plate is placed in the cuboid tank body filling with water.When Seedling grows to 1 leaf 1 heart, by tank body
In water change mill water culture nutrient solution into.When Seedling grows to 2 leaf 1 heart, the nutritional solution in tank body is changed into the Oryza sativa L. battalion containing 0.5%NaCl
Nutrient solution, carries out salt stress process, the nutritional solution changing a saliferous in every 3 days, the salt treatment time is 21 days, after process terminates, puts
Rehydration, after 7 days, can significantly improve the salt tolerance of each material to be tested in the nutritional solution.Peaceful No. 4 salt tolerances of round-grained rice are 1.9 times of comparison,
The fine salt tolerance of Japan is 1.4 times of comparison, pricks 1.9 times (Fig. 2) that Sigma's salt tolerance is comparison.
Claims (5)
1. a kind of method improving Salt Tolerance in Rice Seedlings is it is characterised in that rice paddy seed, after saline seed soaking, makes the resistance to of seedling stage
Salt increases substantially.
2. a kind of method improving Salt Tolerance in Rice Seedlings according to claim 1 is it is characterised in that be to use 1.8%
NaCl seed soaking was soaked seed with fresh water after 1 day.
3. a kind of method improving Salt Tolerance in Rice Seedlings according to claim 2 is it is characterised in that described fresh water soaks
Kind of time is seed soaking to showing money or valuables one carries unintentionally.
4. the authentication method of the Salt Tolerance in Rice Seedlings described in one of claim 1-3 is it is characterised in that be to showing money or valuables one carries unintentionally by seed soaking
Seed afterwards is moved in the PCR plate fined away in 96 holes immediately, the PCR plate with the seed after showing money or valuables one carries unintentionally is placed in and fills with the rectangular of water
In body tank body, when Seedling grows to 1 leaf 1 heart, the water in tank body is changed into mill water culture nutrient solution；When Seedling grows to 2 leaf 1 heart, by tank body
In nutritional solution change rice nutrition liquid containing 0.5%NaCl into, carry out salt stress process, the nutrition changing a saliferous in every 3 days
Liquid, the salt treatment time is 21 days, after process terminates, is placed in rehydration in nutritional solution and, after 7 days, investigates the survival rate of each kind.
5. the authentication method of Salt Tolerance in Rice Seedlings according to claim 4 is it is characterised in that described rice nutrition liquid
Formula contains:N 40mg/L, P 10mg/L, K40mg/L, Ca 40mg/L, Mg 40mg/L, Fe 2mg/L, Mn 0.50mg/L,
Mo 0.05mg/L, B 0.20mg/L, Zn 0.01mg/L, Cu 0.01mg/L, Si 56mg/L.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187863A (en) * | 2018-09-03 | 2019-01-11 | 河北省农林科学院滨海农业研究所 | A kind of greening seedling Seedling Salt-tolerance identification method |
| CN109819714A (en) * | 2019-04-03 | 2019-05-31 | 扬州大学 | A kind of method that can largely screen rice seedling Salinity tolerance germplasm |
| CN110249942A (en) * | 2019-07-29 | 2019-09-20 | 海南广陵高科实业有限公司 | A kind of hybrid rice breeding method |
| CN110731223A (en) * | 2019-11-15 | 2020-01-31 | 南京林业大学 | Method for promoting growth of salix jinguiensis under stress of salts |
| CN112020930A (en) * | 2020-07-31 | 2020-12-04 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN115119734A (en) * | 2022-07-25 | 2022-09-30 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109187863A (en) * | 2018-09-03 | 2019-01-11 | 河北省农林科学院滨海农业研究所 | A kind of greening seedling Seedling Salt-tolerance identification method |
| CN109819714A (en) * | 2019-04-03 | 2019-05-31 | 扬州大学 | A kind of method that can largely screen rice seedling Salinity tolerance germplasm |
| CN110249942A (en) * | 2019-07-29 | 2019-09-20 | 海南广陵高科实业有限公司 | A kind of hybrid rice breeding method |
| CN110249942B (en) * | 2019-07-29 | 2021-07-20 | 海南广陵高科实业有限公司 | Hybrid rice breeding method |
| CN110731223A (en) * | 2019-11-15 | 2020-01-31 | 南京林业大学 | Method for promoting growth of salix jinguiensis under stress of salts |
| CN112020930A (en) * | 2020-07-31 | 2020-12-04 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN112020930B (en) * | 2020-07-31 | 2021-10-08 | 江苏沿海地区农业科学研究所 | Method for inducing rice to improve salt tolerance |
| CN115119734A (en) * | 2022-07-25 | 2022-09-30 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
| CN115119734B (en) * | 2022-07-25 | 2023-08-11 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of plants |
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Application publication date: 20170308 |