CN105474959B - Pinus massoniana tissue culture seedling twig short shoot cutting seedling method - Google Patents
Pinus massoniana tissue culture seedling twig short shoot cutting seedling method Download PDFInfo
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- CN105474959B CN105474959B CN201510905708.6A CN201510905708A CN105474959B CN 105474959 B CN105474959 B CN 105474959B CN 201510905708 A CN201510905708 A CN 201510905708A CN 105474959 B CN105474959 B CN 105474959B
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- 238000005520 cutting process Methods 0.000 title claims abstract description 94
- 235000011609 Pinus massoniana Nutrition 0.000 title claims abstract description 46
- 241000018650 Pinus massoniana Species 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000003337 fertilizer Substances 0.000 claims abstract description 20
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 15
- 230000000366 juvenile effect Effects 0.000 claims abstract description 14
- 238000010828 elution Methods 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims description 24
- 239000007921 spray Substances 0.000 claims description 23
- 238000010899 nucleation Methods 0.000 claims description 18
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 16
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 16
- 230000003020 moisturizing effect Effects 0.000 claims description 16
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 16
- 230000000474 nursing effect Effects 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 12
- 241000737241 Cocos Species 0.000 claims description 10
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 10
- 235000010204 pine bark Nutrition 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 10
- 239000004575 stone Substances 0.000 claims description 10
- 238000007654 immersion Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- 239000007836 KH2PO4 Substances 0.000 claims description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 8
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 8
- 210000003608 fece Anatomy 0.000 claims description 8
- 244000037666 field crops Species 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 229960003512 nicotinic acid Drugs 0.000 claims description 8
- 235000001968 nicotinic acid Nutrition 0.000 claims description 8
- 239000011664 nicotinic acid Substances 0.000 claims description 8
- 229910017464 nitrogen compound Inorganic materials 0.000 claims description 8
- 239000004745 nonwoven fabric Substances 0.000 claims description 8
- 229910052698 phosphorus Inorganic materials 0.000 claims description 8
- 239000011574 phosphorus Substances 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 7
- 239000012286 potassium permanganate Substances 0.000 claims description 7
- 239000002002 slurry Substances 0.000 claims description 7
- 229960002685 biotin Drugs 0.000 claims description 6
- 235000020958 biotin Nutrition 0.000 claims description 6
- 239000011616 biotin Substances 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- 230000008635 plant growth Effects 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 102100040431 Activator of basal transcription 1 Human genes 0.000 claims 1
- 101000964349 Homo sapiens Activator of basal transcription 1 Proteins 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 235000019796 monopotassium phosphate Nutrition 0.000 claims 1
- 235000010333 potassium nitrate Nutrition 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 229940085298 biotin 10 mg Drugs 0.000 description 2
- -1 biotin 10 mg/L Chemical compound 0.000 description 2
- OQVYMXCRDHDTTH-UHFFFAOYSA-N 4-(diethoxyphosphorylmethyl)-2-[4-(diethoxyphosphorylmethyl)pyridin-2-yl]pyridine Chemical compound CCOP(=O)(OCC)CC1=CC=NC(C=2N=CC=C(CP(=O)(OCC)OCC)C=2)=C1 OQVYMXCRDHDTTH-UHFFFAOYSA-N 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
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- 238000012797 qualification Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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Abstract
The invention discloses a pinus massoniana tissue culture seedling twig short shoot cutting seedling method, which comprises the procedures of container selection, matrix preparation, cutting shoot collection, cutting shoot treatment, cutting and nursery management, wherein a proper seedling container is selected, a matrix is prepared according to a certain proportion and a disinfection method, young shoots are collected to be used as cutting shoots, elution and disinfection treatment is carried out, the cutting shoots are directly cut after being treated by a root promoting agent, the control of light, temperature, water and fertilizer conditions is strengthened in the process, the cutting shoots take root about one month, the rooting rate reaches more than 98%, and the survival rate reaches more than 97%. According to the invention, the masson pine tissue culture seedling is used as the scion stock plant for cuttage, so that the problems of long tissue culture subculture period and high seedling raising cost are solved, the propagation multiple is high, the quantity of qualified juvenile scions for cuttage is large, cuttage rooting is easy to occur, the effect is obvious, and the economic benefit, the social benefit and the ecological benefit are good.
Description
Technical field
The invention belongs to technical field of plant propagation, is related to tissue cultivating and seedling technology, especially a kind of masson pine tissue culture
Seedling spray single-node cutting seed-raising method.
Background technology
Masson pine（Pinus massoniana Lamb.）It is south China Major Tree Species Planted, due to the dual-purpose spy of fat material
Property, comprehensive utilization value is high, and promotion prospect is wide.In recent years, as the growing life requirement of people and timber resources are short
Lack and the non-renewable resources such as oil peter out and wait intensification of contradictions, ecology, economic and social sustainable development are increasingly
It is subject to social concerns.Based on China's ecology is peaceful, resource security and the problems such as social safety, the masson pine work main as China
Industry commerical tree species and Tree Species as Bio-energy, it is necessary to greatly develop Masson Pine Plantation.
Guangxi is collected in masson pine germ plasm resource, selection and breeding and store method, belongs to antecedent in the whole nation.From " six or five " national section
Since skill is tackled key problems, a large amount of masson pine choiceness of selection and breeding.Modern forestry is particular about fast growing, high-quality and efficient, is intended to reach
To target, most effective approach is to realize that choiceness nursery stock is afforested.The use of tissue culture, cuttage Fast Asexual Propagation Technique,
It is the effective way for realizing masson pine clone industrialization.
At present, masson pine mother plant for cutting carries out cuttage based on seedling, cuttage seeding, grafting by maternal plant of tissue-cultured seedling
Report it is still fresh rare.Masson pine cuttage root-taking has obvious age effect, i.e. the more easier cuttage root-taking of juvenile form cuttings.Grind
Study carefully discovery, using cuttage, grafting as mother plant for cutting, obtain fringe bar aging seriously, multiple children is difficult, has by force to the elite stand age
Strong Memorability, rooting rate are extremely low；And using the seedling of young age as mother plant for cutting, fringe bar breeding coefficient is low.
The content of the invention
The present invention overcomes cuttings breeding coefficient in existing Cuttage Seedling-Raising of Masson Pine technology is low, rooting rate is undesirable, take root
The technical problems such as effect is unstable, there is provided a kind of qualification cuttings yield is high, cuttage root-taking survival rate is high, effective masson pine
The method of tissue-cultured seedling spray single-node cutting seed-raising.
In order to realize above goal of the invention, technical scheme is as follows：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, including container selection, matrix prepare, cuttings gathers,
Cuttings processing, cuttage and nursery bed management process, select suitable container for plant growth, according to a certain ratio and sterilization method gets out base
After matter, juvenile form spray is collected as cuttings and carries out elution and disinfects, skewer is directly carried out after promoter is handled
Insert, during which strengthen the control of light temperature water and fertilizer condition, Pinus massoniana Seedlings are obtained after carrying out nursery bed management；Its operating procedure is as follows：
（1）Container selects：Selection cave size is the cm of 6 cm × 8, and 70 cave seedlings nursing plates of the cave side with zigzag hole, is made
For cuttage and seedling culture container；
（2）Matrix prepares：Seedling medium is loaded to the non-woven fabrics Seedling bag of the cm size specifications of 8 cm × 9, is subsequently placed in and educates
In seed plate, then the seedling medium installed is drenched water, it is after disinfection to reserve, during cuttage, the water content of seedling medium in Seedling bag
Control is in 65-75%；
（3）Cuttings gathers：Choose and newly take out 3-4 cm long then, 4 more than mm of diameter, first lignifying, top needle is in fasciation
The softness of the shape and juvenile form masson pine spray to flush is cuttings；
（4）Cuttings processing：The cuttings that will be collected, it is rapid to immerse in treatment fluid, carry out at the elution and disinfection of cuttings
Reason, soaking time 5-10 minutes, then with clear water cleaned standby seam；
（5）Cuttage：The cuttings handled well is put into immersion 4-12 h in promoter, then promoter is tuned into talcum powder
Pulpous state, step is inserted directly into after cuttings is dipped in slurry（2）In the seedling medium got ready, by spraying and moisturizing, seedling raising environment phase is controlled
To humidity 90-95 %, 20-30 DEG C of temperature, illumination 1500-2000 lx, 1 month or so cuttings top slightly extracts sprouting, root system shape out
Into obtaining cuttage seeding of taking root；
（6）Nursery bed management：The cuttage seeding that will take root is removed to land for growing field crops, controls light temperature water and fertilizer condition in 1-2 months, and 2
Routinely method for culturing seedlings carries out liquid manure and pest management after a month.
Above step（2）Described in the raw material of seedling medium include fermented- pine bark, coconut palm chaff, yellow soil and floating
Stone, pine bark, coconut palm chaff, yellow soil, float stone are uniformly mixed obtain seedling medium by volume=2: 2: 4: 2.
Above step（2）Described in disinfection be to use volumetric concentration 0.1-0.3 % carbendazim+0.5 % high of volumetric concentration
The mixed liquor of potassium manganate.
Above step（4）The treatment fluid for 1.5 % absolute ethyl alcohols of volumetric concentration+0.1 % malonic acid of volumetric concentration+
The mixed solution of volumetric concentration 0.1-0.2 % Mancozebs+0.0 2% streptomysin of volumetric concentration.
Above step（5）The promoter be containing：NAA 50-100 mg/L、IBA 0-50 mg/L、ABT1 0-50
mg/L、6-BA 5-10 mg/L、VB1 50-100 mg/L、VB6 20-50 mg/L, nicotinic acid 20-50 mg/L, biotin 10-30
mg/L、KNO3 850 mg/L、KH2PO4The mix preparation of 240 mg/L.
Above step（6）In the nursery bed management, in the 1st month, shade is paid attention to, illumination is controlled in 1500-2000
Lx, at 20-30 DEG C, relative humidity 75-85 %, are answered temperature every 7-10 d with the high phosphorus of 1 ‰ 6N-35P-10K of volumetric concentration
Hefei carries out Foliar Spraying；In 2nd month, moisturizing of divulging information is paid attention to, relative humidity 60-70 % are dense every 10-14 d volumes
The high-nitrogen compound fertilizer for spending ‰ 30N-13P-8K of 1-3 carries out Foliar Spraying.
Compared with the prior art, advantages of the present invention and good effect are as follows：
1st, the present invention is using masson pine tissue-cultured seedling as mother plant for cutting, and sprout juvenile formization is obvious, flushes, and breeding coefficient is
10-30, for one month rooting rate of cuttage cuttings up to more than 98%, survival rate more than 97%, drastically increases the production of qualified cuttings
Amount and cutting experiment, have obvious progressive, remarkable benefit.
2nd, the present invention according to masson pine tissue-cultured seedling growth characteristics, it is determined that adapt to Cuttage Seedling-Raising of Masson Pine containers size,
The technical measures such as substrate composition, cuttings type, cuttings processing method, the proportioning of rooting nutrition solution, and nursery stock management after cuttage is said
Bright, whole technically reliable, easy to operate, nursery effect is good, and achievements conversion potentiality are big, and popularizing application prospect is wide.
3rd, the present invention using masson pine tissue-cultured seedling for mother plant for cutting carry out cuttage, on the one hand overcome tissue culture subculture cycle length,
The problem of seedling cost is higher, while breeding rate is high, more for the qualified juvenile form fringe bar quantity of cuttage, easily cuttage is given birth to
Root, significant effect, has preferable economic benefit, social benefit and ecological benefits.
Attached drawing identifies
Fig. 1 is masson pine tissue-cultured seedling mother plant for cutting in the present invention.
Fig. 2 is taken root cuttage seeding for masson pine in the present invention.
Fig. 3 is the masson pine cutting survival seedling that the present invention obtains.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.1 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 65%.
Choose and newly take out 3 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.1 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 10 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 50 mg/L, 6-BA 5 mg/L, VB1 50 mg/L、VB6 20 mg/L、
20 mg/L of nicotinic acid, biotin 10 mg/L, KNO3 850 mg/L、KH2PO4The promoter that the mix preparation of 240 mg/L is formed
Promoter, is then tuned into pulpous state by 12 h of middle immersion with talcum powder, is inserted directly into after cuttings is dipped in slurry in the seedling medium got ready,
By spraying and moisturizing, seedling raising environment relative humidity 90-95 %, 20-30 DEG C of temperature, illumination 1500-2000 lx, 1 month left side are controlled
Sprouting is slightly extracted on right cuttings top out, and root system is formed, and obtains cuttage seeding of taking root, rooting rate 98.3%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 20-30 DEG C, relative humidity 75-85 %, are carried out degree every 7 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 10 d, 3 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 97.8% to count Pinus massoniana Seedlings survival rate.
Embodiment 2：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.3 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 75%.
Choose and newly take out 4 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.2 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 5 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 100 mg/L, 6-BA 10 mg/L, VB1 100 mg/L、VB6 50
Mg/L, 50 mg/L of nicotinic acid, biotin 30 mg/L, KNO3 850 mg/L、KH2PO4What the mix preparation of 240 mg/L was formed
4 h are soaked in promoter, promoter is then tuned into pulpous state with talcum powder, cuttings is dipped in the nursery base for being inserted directly into and getting ready after starching
In matter, by spraying and moisturizing, seedling raising environment relative humidity 90-95 % are controlled, 20-30 DEG C of temperature, illumination 1500-2000 lx, 1
Sprouting is slightly extracted on cuttings top out within a month or so, and root system is formed, and obtains cuttage seeding of taking root, rooting rate 98.1%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 20-30 DEG C, relative humidity 75-85 %, are carried out degree every 10 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 14 d, 1 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 97.0% to count Pinus massoniana Seedlings survival rate.
Embodiment 3：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.2 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 70%.
Choose and newly take out 3 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.1 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 10 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 50 mg/L, IBA 50 mg/L, ABT150 mg/L、6-BA 5 mg/
L、VB1 50 mg/L、VB6 20 mg/L, 20 mg/L of nicotinic acid, biotin 10 mg/L, KNO3 850 mg/L、KH2PO4 240
12 h are soaked in the promoter that the mix preparation of mg/L is formed, promoter is then tuned into pulpous state with talcum powder, cuttings is dipped in into slurry
It is inserted directly into afterwards in the seedling medium got ready, passes through spraying and moisturizing, control seedling raising environment relative humidity 90-95 %, temperature 20-25
DEG C, illumination 1500-2000 lx, sprouting is slightly extracted on cuttings top out within 1 month or so, and root system is formed, and obtains cuttage seeding of taking root, rooting rate
For 98%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 20-25 DEG C, relative humidity 75-85 %, are carried out degree every 8 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 10 d, 2 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 99.2% to count Pinus massoniana Seedlings survival rate.
Embodiment 4：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.1 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 65%.
Choose and newly take out 3 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.2 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 5 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 100 mg/L, IBA50 mg/L, ABT1 50 mg/L、6-BA 10
mg/L、VB1 100 mg/L、VB6 50 mg/L, 50 mg/L of nicotinic acid, biotin 30 mg/L, KNO3 850 mg/L、KH2PO4
4 h are soaked in the promoter that the mix preparation of 240 mg/L is formed, promoter is then tuned into pulpous state with talcum powder, cuttings is dipped in
It is inserted directly into after slurry in the seedling medium got ready, passes through spraying and moisturizing, control seedling raising environment relative humidity 90-95 %, temperature 25-
30 DEG C, illumination 1500-2000 lx, sprouting is slightly extracted on cuttings top out within 1 month or so, and root system is formed, and obtains cuttage seeding of taking root, and is taken root
Rate is 98.2%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 25-30 DEG C, relative humidity 75-85 %, are carried out degree every 10 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 12 d, 2 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 97.5% to count Pinus massoniana Seedlings survival rate.
Embodiment 5：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.2 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 70%.
Choose and newly take out 4 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.1 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 10 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 50 mg/L, IBA 50 mg/L, ABT1 50 mg/L、6-BA 10
mg/L、VB1 100 mg/L、VB6 30 mg/L, 30 mg/L of nicotinic acid, biotin 20 mg/L, KNO3 850 mg/L、KH2PO4
8 h are soaked in the promoter that the mix preparation of 240 mg/L is formed, promoter is then tuned into pulpous state with talcum powder, cuttings is dipped in
It is inserted directly into after slurry in the seedling medium got ready, passes through spraying and moisturizing, control seedling raising environment relative humidity 90-95 %, temperature 20-
30 DEG C, illumination 1500-2000 lx, sprouting is slightly extracted on cuttings top out within 1 month or so, and root system is formed, and obtains cuttage seeding of taking root, and is taken root
Rate is 99.4%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 20-30 DEG C, relative humidity 75-85 %, are carried out degree every 10 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 14 d, 3 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 98.9% to count Pinus massoniana Seedlings survival rate.
Embodiment 6：
A kind of masson pine tissue-cultured seedling spray single-node cutting seed-raising method, selection cave size are the cm of 6 cm × 8, and cave side band
70 cave seedlings nursing plates of zigzag hole, as cuttage and seedling culture container.Body is pressed with fermented- pine bark, coconut palm chaff, yellow soil, float stone
Product ratio=2: 2: 4: 2, which are uniformly mixed, obtains seedling medium, and the non-woven fabrics that seedling medium is loaded to the cm size specifications of 8 cm × 9 is educated
Seedling bag, is subsequently placed in seedlings nursing plate, then the seedling medium installed is drenched water, using 0.3 % carbendazim of volumetric concentration+volume
The mixed liquor of 0.5 % potassium permanganate of concentration is after disinfection to reserve to seedling medium, during cuttage, the water content of seedling medium in Seedling bag
Control is 75%.
Choose and newly take out 3 cm long then, 4 more than mm of diameter, first lignifying, top needle is in the softness and vigor of fasciation shape
Vigorous juvenile form masson pine spray is cuttings；The cuttings that will be collected, 1.5 % absolute ethyl alcohols of rapid immersion volumetric concentration+
The place that 0.1 % malonic acid of volumetric concentration+0.2 % Mancozebs of volumetric concentration+0.0 2% streptomysin of volumetric concentration is obtained by mixing
Manage in liquid, carry out the elution of cuttings and disinfect.Soaking time 5 minutes in treatment fluid, then with clear water cleaned standby seam.
The cuttings handled well is put into containing NAA 75 mg/L, IBA 25 mg/L, ABT1 25 mg/L、6-BA 10
mg/L、VB1 75 mg/L、VB6 40 mg/L, 40 mg/L of nicotinic acid, biotin 20 mg/L, KNO3 850 mg/L、KH2PO4
10 h are soaked in the promoter that the mix preparation of 240 mg/L is formed, promoter are then tuned into pulpous state with talcum powder, by cuttings
It is inserted directly into after dipping in slurry in the seedling medium got ready, by spraying and moisturizing, controls seedling raising environment relative humidity 90-95 %, temperature
20-30 DEG C, illumination 1500-2000 lx, sprouting is slightly extracted on cuttings top out within 1 month or so, and root system is formed, and obtains cuttage seeding of taking root, raw
Root rate is 98.6%.
The cuttage seeding that will take root is removed to land for growing field crops, in the 1st month, pays attention to shade, illumination control is in 1500-2000 lx, temperature
At 20-30 DEG C, relative humidity 75-85 %, are carried out degree every 9 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration
Foliar Spraying；In 2nd month, moisturizing of divulging information, relative humidity 60-70 %, every 12 d, 3 ‰ 30N- of volumetric concentration are paid attention to
The high-nitrogen compound fertilizer of 13P-8K carries out Foliar Spraying；Routinely method for culturing seedlings carries out liquid manure and pest management, system after 2 months
It is 97.5% to count Pinus massoniana Seedlings survival rate.
Claims (3)
- A kind of 1. masson pine tissue-cultured seedling spray single-node cutting seed-raising method, it is characterised in that：Including container selection, matrix prepare, Cuttings collection, cuttings processing, cuttage and nursery bed management process, select suitable container for plant growth, according to a certain ratio and disinfection side After method gets out matrix, juvenile form spray is collected as cuttings and carries out elution and disinfects, after promoter is handled Cuttage is directly carried out, during which strengthens the control of light temperature water and fertilizer condition, Pinus massoniana Seedlings are obtained after carrying out nursery bed management；It operates step It is rapid as follows：（1）Container selects：Selection cave size is the cm of 6 cm × 8, and 70 cave seedlings nursing plates of the cave side with zigzag hole, as skewer Plug in container for plant growth；（2）Matrix prepares：Seedling medium is loaded to the non-woven fabrics Seedling bag of the cm size specifications of 8 cm × 9, is subsequently placed in seedlings nursing plate In, then the seedling medium installed is drenched water, it is after disinfection to reserve, during cuttage, the water content control of seedling medium in Seedling bag In 65-75%；（3）Cuttings gathers：Choose and newly take out 3-4 cm long then, 4 more than mm of diameter, first lignifying, top needle is in fasciation shape Juvenile form masson pine spray that is soft and flushing is cuttings；（4）Cuttings processing：The cuttings that will be collected, it is rapid to immerse in treatment fluid, carry out the elution of cuttings and disinfect, Soaking time 5-10 minutes, then with clear water cleaned standby seam；（5）Cuttage：The cuttings handled well is put into immersion 4-12 h in promoter, then promoter is slurred with talcum powder Shape, step is inserted directly into after cuttings is dipped in slurry（2）It is opposite by spraying and moisturizing, control seedling raising environment in the seedling medium got ready Humidity 90-95 %, 20-30 DEG C of temperature, illumination 1500-2000 lx, sprouting is slightly extracted on cuttings top out within 1 month or so, and root system is formed, Obtain cuttage seeding of taking root；（6）Nursery bed management：The cuttage seeding that will take root is removed to land for growing field crops, controls light temperature water and fertilizer condition in 1-2 months, 2 months Routinely method for culturing seedlings carries out liquid manure and pest management afterwards；Step（2）Described in the raw material of seedling medium include fermented- pine bark, coconut palm chaff, yellow soil and float stone, pine bark, Coconut palm chaff, yellow soil, float stone are uniformly mixed obtain seedling medium by volume=2: 2: 4: 2；Step（4）The treatment fluid is 1.5 % absolute ethyl alcohols of volumetric concentration+0.1 % malonic acid of volumetric concentration+volumetric concentration The mixed solution of 0.0 2% streptomysin of 0.1-0.2 % Mancozebs+volumetric concentration；Step（5）The promoter be containing：NAA 50-100 mg/L、IBA 0-50 mg/L、ABT1 0-50 mg/L、6- BA 5-10 mg/L, VB1 50-100 mg/L, VB6 20-50 mg/L, nicotinic acid 20-50 mg/L, biotin 10-30 mg/L, KNO3 850 mg/L, KH2PO4 240 mg/L mix preparation.
- 2. masson pine tissue-cultured seedling spray single-node cutting seed-raising method according to claim 1, it is characterised in that：Step（2） Described in disinfection be mixed liquor using volumetric concentration 0.1-0.3 % carbendazim+0.5 % potassium permanganate of volumetric concentration.
- 3. masson pine tissue-cultured seedling spray single-node cutting seed-raising method according to claim 1, it is characterised in that：Step（6） In the nursery bed management, in the 1st month, shade is paid attention to, illumination is controlled in 1500-2000 lx, and temperature is at 20-30 DEG C, relatively Humidity 75-85 %, Foliar Spraying is carried out every 7-10 d with the high phosphorus composite fertilizer of 1 ‰ 6N-35P-10K of volumetric concentration；2nd In a month, moisturizing of divulging information, relative humidity 60-70 %, every 10-14 d with ‰ 30N-13P-8K's of volumetric concentration 1-3 are paid attention to High-nitrogen compound fertilizer carries out Foliar Spraying.
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| CN105875375A (en) * | 2016-04-18 | 2016-08-24 | 广西壮族自治区林业科学研究院 | Soilless substrate seedling-raising method of keteleeria cyclolepis |
| CN106212273B (en) * | 2016-07-15 | 2018-03-13 | 广西壮族自治区林业科学研究院 | Promote masson pine epicormic branch cuttage take root liquid fertilizer, preparation method and applications |
| CN106165634B (en) * | 2016-07-15 | 2021-06-08 | 广西壮族自治区林业科学研究院 | Seedling growing method for promoting asexual seedling growth of pinus massoniana |
| CN106134904A (en) * | 2016-07-28 | 2016-11-23 | 杭州凌萤科技有限公司 | Pinus massoniana Lamb fast seedling-cultivating method |
| CN109156185A (en) * | 2018-09-03 | 2019-01-08 | 广西壮族自治区林业科学研究院 | A kind of masson pine Water culture method for culturing seedlings |
| CN109566126A (en) * | 2018-12-25 | 2019-04-05 | 福建农林大学 | A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication |
| CN109566622B (en) * | 2019-01-08 | 2021-04-06 | 广西壮族自治区林业科学研究院 | Pine cuttage rooting agent and preparation method and use method thereof |
| CN110558071A (en) * | 2019-10-14 | 2019-12-13 | 广西壮族自治区林业科学研究院 | Cutting seedling method for olive twigs |
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