CN109566126A - A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication - Google Patents
A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication Download PDFInfo
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- CN109566126A CN109566126A CN201811594491.1A CN201811594491A CN109566126A CN 109566126 A CN109566126 A CN 109566126A CN 201811594491 A CN201811594491 A CN 201811594491A CN 109566126 A CN109566126 A CN 109566126A
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- pearl color
- cuttage
- color osmanthus
- seedling
- tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication, including cuttage outside test material preparation, the pretreatment of cutting medium, cuttings processing, Pearl color osmanthus tissue-cultured seedling bottle, the tissue culture seedling rooting management of Pearl color osmanthus.The present invention expands numerous a large amount of sterile Pearl color osmanthus single plant by tissue cultures approach, establish the mode that Pearl color osmanthus carries out cutting propagation throughout the year, the prompt phenanthrene Jiffy nutrient matrix seedling growing block of this mode cuttage Norway import, eliminate the transplanting and management process in later period, not only simplify cuttage process, it reduces costs, and then sets up a whole set of efficient Pearl color osmanthus seedling-raising technique.The method can directly apply to nursery lot or directly afforestation uses with forming seedling through one step culture after taking root.
Description
Technical field
The present invention relates to a kind of methods of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication.
Background technique
Pearl color osmanthus is the mutation of wooden slippers category sweet osmanthus, and leaf is oval sawtooth, and blade is compared to common greenery sweet osmanthus
It is thicker.Leaf can be with perenniaity turn colors.Early spring tender shoots be purplish red rhodo, after switch to it is pink, and then turn golden yellow
To white.Started again from central main lobe arteries and veins to both sides gradual change green.Until dark green, one Nian Si under conditions of weather conditions are suitable for
Season constantly has tender shoots to emerge, therefore entire plant pearl is of a riot of colour from top to bottom, deep purples and bright reds-beautiful flowersa riot of brilliant purple and tender crimson.
Correlative study and report currently without Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication.
The technical solution adopted by the present invention is as follows:
A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication, comprising the following steps:
1. test material prepares
By the Pearl color osmanthus tissue-cultured seedling of tissue culture squamous subculture, the unrooted by plant height 5cm or so, with 2 pairs with blade is chosen
Strong sprout is cut on superclean bench, and the residual MS culture medium on plant surface is cleaned in clear water, is then put in clear water and protects
It is wet, it is spare.
2. cutting medium and pretreatment
Cuttage material is the prompt phenanthrene Jiffy nutrient matrix seedling growing block of Norway's import, by the prompt phenanthrene Jiffy nutrient matrix nursery of Norway's import
Block is immersed in water, is taken out after substrate block absorbs water expand completely, is drained the moisture in substrate block, no longer dripped with substrate block for
Standard is dried, spare.
3. cuttings is handled
The spare Pearl color osmanthus tissue-cultured seedling single plant base portion of moisturizing is inserted into the IBA solution of prepared 3mg/L, is taken after impregnating 2h
Out, moisturizing, it is spare.
4. the outer cuttage of Pearl color osmanthus tissue-cultured seedling bottle
The good substrate block of cuttage pre-treatment is uniformly placed on the seedbed of cuttage greenhouse, using bamboo stick by substrate block when cuttage
Perforating (fineness degree of bamboo stick should be similar with the rugosity of Pearl color osmanthus tissue culture seeding stem segment), perforating depth 2cm or so, by step 3 place
The Pearl color osmanthus tissue-cultured seedling single plant managed inserts in the eye that bamboo stick is beaten, and is compressed the matrix around fringe bar with hand after cuttage, cuttage
Afterwards, it is drenched the seedbed with watering can.
5. the tissue culture seedling rooting management of Pearl color osmanthus
Automatic sprinkling system is prepared in cuttage greenhouse, every 15-30min, automatic irrigation is primary, to guarantee sprinkling irrigation uniformly, water spray
System is using rotary sprinkling irrigation, and after sensor connects solenoid valve, water spray drives nozzle in 360 ° of rotations, and spray head is every 1 meter ampere
Set one.Cuttage greenhouse is in arcuation, has plastic film above, when fine day, plastic film is rolled, to keep ventilation saturating
Light rains and has wind weather to pull down plastic film, and rainwater is avoided to drench seedbed pollution nursery stock.
Every 15 days early in the morning or at dusk after cuttage, the carbendazim or methyl Tobe for being 0.1wt%-0.5wt% with concentration
Saliva sprays blade disinfection once, and automatic sprinkling system starts to spray water again after 5 hours.Withered branch is cleared up, in time to avoid sense
Contaminate other branches.
10d or so after Pearl color osmanthus is taken root, with the ammonium nitrate of 0.5wt% and 0.5wt% potassium dihydrogen phosphate mixed solution into
Row foliage-spray is grown with the element that supplements the nutrients for seedling.
6. Pearl color osmanthus tissue culture seedling rooting counts
35-40d counts the situation of taking root of Pearl color osmanthus tissue-cultured seedling after cuttage, and statistics Pearl color osmanthus tissue-cultured seedling rooting rate is 100%.
Remarkable result of the invention is:
The present invention expands numerous a large amount of sterile Pearl color osmanthus single plant by tissue cultures approach, establish Pearl color osmanthus throughout the year into
The mode of row cutting propagation, the prompt phenanthrene Jiffy nutrient matrix seedling growing block of this mode cuttage Norway import, eliminates the shifting in later period
Management process is planted, cuttage process is not only simplified, reduces costs, and then set up a whole set of efficient Pearl color osmanthus nursery skill
Art.The method can directly apply to nursery lot or directly afforestation uses with forming seedling through one step culture after taking root.
Simple, easy to operate, at low cost using the prompt phenanthrene Jiffy nutrient matrix cuttage process of Norway's import, which has
Ventilative, water conservation, moisturizing, and plant nutrient can be provided, and substrate block is sterilized, it can directly use, save general skewer
The process of inserting matrix.Can be with save the cost and simple flow using single auxin IBA, and root induction rate is higher.
Specific embodiment
Below will by example, the invention will be further described, these descriptions are not to make to the content of present invention into one
The restriction of step.It should be understood by those skilled in the art that equivalent replacement made by the content of present invention, or be correspondingly improved, still belong to
Within protection scope of the present invention.
Embodiment 1
A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication, comprising the following steps:
1. test material prepares
By the Pearl color osmanthus tissue-cultured seedling of tissue culture squamous subculture, the unrooted by plant height 5cm or so, with 2 pairs with blade is chosen
Strong sprout is cut on superclean bench, and the residual MS culture medium on plant surface is cleaned in clear water, is then put in clear water and protects
It is wet, it is spare.
2. cutting medium and pretreatment
Cuttage material is the prompt phenanthrene Jiffy nutrient matrix seedling growing block of Norway's import, by the prompt phenanthrene Jiffy nutrient matrix nursery of Norway's import
Block is immersed in water, is taken out after substrate block absorbs water expand completely, is drained the moisture in substrate block, no longer dripped with substrate block for
Standard is dried, spare.
3. cuttings is handled
The spare Pearl color osmanthus tissue-cultured seedling single plant base portion of moisturizing is inserted into the IBA solution of prepared 3mg/L, is taken after impregnating 2h
Out, moisturizing, it is spare.
4. the outer cuttage of Pearl color osmanthus tissue-cultured seedling bottle
The good substrate block of cuttage pre-treatment is uniformly placed on the seedbed of cuttage greenhouse, using bamboo stick by substrate block when cuttage
Perforating (fineness degree of bamboo stick should be similar with the rugosity of Pearl color osmanthus tissue culture seeding stem segment), perforating depth 2cm or so, by step 3 place
The Pearl color osmanthus tissue-cultured seedling single plant managed inserts in the eye that bamboo stick is beaten, and is compressed the matrix around fringe bar with hand after cuttage, cuttage
Afterwards, it is drenched the seedbed with watering can.
5. the tissue culture seedling rooting management of Pearl color osmanthus
Automatic sprinkling system is prepared in cuttage greenhouse, every 30min, automatic irrigation is primary, to guarantee sprinkling irrigation uniformly, water injection system
Using rotary sprinkling irrigation, after sensor connects solenoid valve, water spray drives nozzle in 360 ° of rotations, and spray head sets one every 1 meter ampere
It is a.Cuttage greenhouse is in arcuation, has plastic film above, and when fine day, plastic film is rolled, to keep ventilation light transmission,
It rains and there is wind weather to pull down plastic film, rainwater is avoided to drench seedbed pollution nursery stock.
Every 15 days early in the morning after cuttage, the carbendazim sprinkling blade disinfection for being 0.1wt% with concentration is primary, after 5 hours
Automatic sprinkling system starts to spray water again.Withered branch is cleared up, in time to avoid other branches are infected.
10d or so after Pearl color osmanthus is taken root, with the ammonium nitrate of 0.5wt% and 0.5wt% potassium dihydrogen phosphate mixed solution into
Row foliage-spray is grown with the element that supplements the nutrients for seedling.
6. Pearl color osmanthus tissue culture seedling rooting counts
35-40d counts the situation of taking root of Pearl color osmanthus tissue-cultured seedling after cuttage, and statistics Pearl color osmanthus tissue-cultured seedling rooting rate is 100%.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, is all covered by the present invention.
Claims (2)
1. a kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication, it is characterised in that: the following steps are included:
(1) test material prepares
By the Pearl color osmanthus tissue-cultured seedling of tissue culture squamous subculture, the unrooted strong sprout by plant height 5cm, with 2 pairs with blade is chosen,
It is cut on superclean bench, the residual MS culture medium on plant surface is cleaned in clear water, is then put in moisturizing in clear water, it is standby
With；
(2) pretreatment of cutting medium
Substrate block is immersed in water, is taken out after substrate block absorbs water expand completely, the moisture in substrate block is drained, with substrate block
Subject to no longer dripping, dry, it is spare；
(3) cuttings is handled
The spare Pearl color osmanthus tissue-cultured seedling single plant base portion of moisturizing is inserted into the IBA solution of prepared 3mg/L, is taken after impregnating 2h
Out, moisturizing, it is spare；
(4) the outer cuttage of Pearl color osmanthus tissue-cultured seedling bottle
The substrate block handled well is uniformly placed on the seedbed of cuttage greenhouse, using bamboo stick by substrate block perforating when cuttage,
Perforating depth 2cm inserts in the Pearl color osmanthus tissue-cultured seedling single plant that step (3) is handled well in the eye that bamboo stick is beaten, will with hand after cuttage
Matrix around fringe bar compresses, and after cuttage, is drenched the seedbed with watering can；
(5) Pearl color osmanthus tissue culture seedling rooting management
Automatic sprinkling system is prepared in cuttage greenhouse, every 15-30min, automatic irrigation is primary, to guarantee sprinkling irrigation uniformly, water spray
System is using rotary sprinkling irrigation, and after sensor connects solenoid valve, water spray drives nozzle in 360 ° of rotations, and spray head is every 1 meter ampere
Set one；Cuttage greenhouse is in arcuation, has plastic film above, when fine day, plastic film is rolled, to keep ventilation saturating
Light rains and has wind weather to pull down plastic film, and rainwater is avoided to drench seedbed pollution nursery stock；
Every 15 days early in the morning or at dusk after cuttage, the carbendazim or thiophanate methyl for being 0.1wt%-0.5wt% with concentration spray
Spill blade disinfection once, automatic sprinkling system starts to spray water again after 5 hours, clears up withered branch in time；
Pearl color osmanthus take root after 10d, carry out blade face with the ammonium nitrate of 0.5wt% and 0.5wt% potassium dihydrogen phosphate mixed solution
It sprays, is grown for seedling.
2. a kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication according to claim 1, it is characterised in that: the matrix
Block is the prompt phenanthrene Jiffy nutrient matrix seedling growing block of Norway's import.
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| CN201811594491.1A CN109566126A (en) | 2018-12-25 | 2018-12-25 | A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication |
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| CN201811594491.1A CN109566126A (en) | 2018-12-25 | 2018-12-25 | A kind of method of Pearl color osmanthus tissue culture outside sprout-cultivating-bottle radication |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111512808A (en) * | 2019-10-30 | 2020-08-11 | 福建农林大学 | Method for rapidly inducing adventitious root regeneration of brachypodium distachyon tissue culture seedlings |
| CN114208517A (en) * | 2022-01-05 | 2022-03-22 | 南昌工程学院 | Rapid rooting method for citral type cinnamomum longepaniculatum test-tube plantlets |
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| CN114208517A (en) * | 2022-01-05 | 2022-03-22 | 南昌工程学院 | Rapid rooting method for citral type cinnamomum longepaniculatum test-tube plantlets |
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