CN105474959A - Pinus massoniana tissue culture seedling twig short shoot cutting seedling method - Google Patents
Pinus massoniana tissue culture seedling twig short shoot cutting seedling method Download PDFInfo
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- CN105474959A CN105474959A CN201510905708.6A CN201510905708A CN105474959A CN 105474959 A CN105474959 A CN 105474959A CN 201510905708 A CN201510905708 A CN 201510905708A CN 105474959 A CN105474959 A CN 105474959A
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- seedling
- cuttings
- cuttage
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- masson pine
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- 238000005520 cutting process Methods 0.000 title claims abstract description 97
- 235000011609 Pinus massoniana Nutrition 0.000 title claims abstract description 49
- 241000018650 Pinus massoniana Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 39
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000003337 fertilizer Substances 0.000 claims abstract description 19
- 230000000366 juvenile effect Effects 0.000 claims abstract description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- 239000011159 matrix material Substances 0.000 claims abstract description 8
- 239000007921 spray Substances 0.000 claims description 26
- 238000005507 spraying Methods 0.000 claims description 24
- 238000000338 in vitro Methods 0.000 claims description 21
- 238000010899 nucleation Methods 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 17
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 16
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 16
- 230000003020 moisturizing effect Effects 0.000 claims description 16
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims description 16
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 241000737241 Cocos Species 0.000 claims description 10
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 10
- 239000002689 soil Substances 0.000 claims description 10
- 239000004575 stone Substances 0.000 claims description 10
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 239000005802 Mancozeb Substances 0.000 claims description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 8
- 241000607479 Yersinia pestis Species 0.000 claims description 8
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- 239000002131 composite material Substances 0.000 claims description 8
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 8
- 210000003608 fece Anatomy 0.000 claims description 8
- 244000037666 field crops Species 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 229960003512 nicotinic acid Drugs 0.000 claims description 8
- 235000001968 nicotinic acid Nutrition 0.000 claims description 8
- 239000011664 nicotinic acid Substances 0.000 claims description 8
- 229910017464 nitrogen compound Inorganic materials 0.000 claims description 8
- 239000004745 nonwoven fabric Substances 0.000 claims description 8
- 229910052698 phosphorus Inorganic materials 0.000 claims description 8
- 239000011574 phosphorus Substances 0.000 claims description 8
- 239000012286 potassium permanganate Substances 0.000 claims description 8
- 230000002786 root growth Effects 0.000 claims description 8
- 239000002002 slurry Substances 0.000 claims description 8
- 229960005322 streptomycin Drugs 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 229940088594 vitamin Drugs 0.000 claims description 8
- 235000013343 vitamin Nutrition 0.000 claims description 8
- 239000011782 vitamin Substances 0.000 claims description 8
- 229930003231 vitamin Natural products 0.000 claims description 8
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 8
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000011017 operating method Methods 0.000 claims description 2
- 235000010204 pine bark Nutrition 0.000 claims description 2
- 230000008635 plant growth Effects 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 10
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 230000008901 benefit Effects 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000007654 immersion Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000550 effect on aging Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/78—1,3-Thiazoles; Hydrogenated 1,3-thiazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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Abstract
The invention discloses a pinus massoniana tissue culture seedling twig short shoot cutting seedling method, which comprises the procedures of container selection, matrix preparation, cutting shoot collection, cutting shoot treatment, cutting and nursery management, wherein a proper seedling container is selected, a matrix is prepared according to a certain proportion and a disinfection method, young shoots are collected to be used as cutting shoots, elution and disinfection treatment is carried out, the cutting shoots are directly cut after being treated by a root promoting agent, the control of light, temperature, water and fertilizer conditions is strengthened in the process, the cutting shoots take root about one month, the rooting rate reaches more than 98%, and the survival rate reaches more than 97%. According to the invention, the masson pine tissue culture seedling is used as the scion stock plant for cuttage, so that the problems of long tissue culture subculture period and high seedling raising cost are solved, the propagation multiple is high, the quantity of qualified juvenile scions for cuttage is large, cuttage rooting is easy to occur, the effect is obvious, and the economic benefit, the social benefit and the ecological benefit are good.
Description
Technical field
The invention belongs to technical field of plant propagation, relate to tissue cultivating and seedling technology, especially a kind of masson pine plantlet in vitro spray single-node cutting seed-raising method.
Background technology
Masson pine (
pinusmassonianalamb.) be south China Major Tree Species Planted, due to the dual-purpose characteristic of fat material, comprehensive utilization value is high, and promotion prospect is wide.In recent years, the life requirement growing along with people and the non-renewable resources such as timber resource shortage and oil such as to be petered out at the intensification of contradictions, and ecological, economic and social sustainable development is more and more subject to social concerns., the problem such as resource security and social safety peaceful based on China's ecology, masson pine, as the main industrial cut stock seeds of China and Tree Species as Bio-energy, greatly develops Masson Pine Plantation necessary.
Guangxi, at the collection of masson pine germ plasm resource, seed selection and store method, belongs to antecedent in the whole nation.Since " six or five " National Key Research Programs, a large amount of masson pine choiceness of seed selection.Modern forestry is particular about fast growing, high-quality and efficient, and for reaching re-set target, the most effective approach realizes choiceness nursery stock afforestation.The use of group training, cuttage Fast Asexual Propagation Technique is the effective way realizing the industrialization of masson pine clone.
At present, masson pine mother plant for cutting is based on seedling, cuttage seeding, grafting, and it is still fresh rare with plantlet in vitro to be that maternal plant carries out the report of cuttage.Masson pine cuttage root-taking has obvious age effect, i.e. the more easier cuttage root-taking of juvenile form cuttings.Research finds, using cuttage, grafting as mother plant for cutting, institute's fringe bar aging that obtains is serious, multiple children's difficulty, and have strong Memorability to the elite stand age, rooting rate is extremely low; And using the seedling in young age as mother plant for cutting, fringe bar reproduction coefficient is low.
Summary of the invention
Instant invention overcomes in existing Cuttage Seedling-Raising of Masson Pine technology that cuttings reproduction coefficient is low, rooting rate is undesirable, the technical problems such as rooting efficiency is unstable, provide that a kind of qualified cuttings output is high, cuttage root-taking survival rate is high, the method for the masson pine plantlet in vitro spray single-node cutting seed-raising of Be very effective.
In order to realize above goal of the invention, technical scheme of the present invention is as follows:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, comprise selection of container, matrix preparation, cuttings collection, cuttings process, cuttage and nursery bed management operation, select suitable container for plant growth, according to a certain ratio and after sterilization method gets out matrix, juvenile form spray is collected as cuttings and carries out wash-out and disinfect, after rooting promoter process, directly carry out cuttage, period adds the control of the fertile condition of high light warm water, obtains Pinus massoniana Seedlings after carrying out nursery bed management; Its operating procedure is as follows:
(1) selection of container: select cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container;
(2) matrix prepares: non-woven fabrics Seedling bag seedling medium being loaded 8cm × 9cm size specification, then seedling-cultivating tray is placed in, water of then being drenched by the seedling medium installed, for subsequent use after sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is at 65-75%;
(3) cuttings collection: choose that newly to take out 3-4cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings;
(4) cuttings process: by the cuttings collected, immerses in treatment fluid rapidly, carries out the wash-out of cuttings and disinfect, soak time 5-10 minute, then using clear water cleaned standby seam;
(5) cuttage: the cuttings handled well is put into rooting promoter and soaks 4-12h, then use talcum powder rooting promoter furnishing pulpous state, in the seedling medium that after cuttings being dipped in slurry, directly inserting step (2) is got ready, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, and root growth, obtains cuttage seeding of taking root;
(6) nursery bed management: cuttage seeding of taking root is removed to nursery, land for growing field crops, controls light temperature water and fertilizer condition well in 1-2 month, after 2 months, seedling-cultivating method carries out liquid manure and pest management routinely.
The raw material of the seedling medium described in above step (2) comprises fermented-pine bark, coconut palm chaff, yellow soil and float stone, and pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2 mix and obtain seedling medium.
Sterilization described in above step (2) is the mixed liquor adopting volumetric concentration 0.1-0.3% carbendazim+volumetric concentration 0.5% potassium permanganate.
Treatment fluid described in above step (4) is the mixed solution of volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1-0.2% mancozeb+volumetric concentration 0.02% streptomycin.
Rooting promoter described in above step (5) is for containing: NAA50-100mg/L, IBA0-50mg/L, ABT
10-50mg/L, 6-BA5-10mg/L, VB
150-100mg/L, VB
620-50mg/L, nicotinic acid 20-50mg/L, vitamin h 10-30mg/L, KNO
3850mg/L, KH
2pO
4the mix preparation of 240mg/L.
In nursery bed management described in above step (6), in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 7-10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 10-14d volumetric concentration 1-3 ‰ 30N-13P-8K carries out Foliar Spraying.
Compared to prior art, advantage of the present invention and good effect as follows:
1, the present invention is using masson pine plantlet in vitro as mother plant for cutting, bud seedling juvenile formization is obvious, flush, reproduction coefficient is 10-30, a cuttage cuttings month rooting rate reaches more than 98%, survival rate more than 97%, drastically increases output and the cutting experiment of qualified cuttings, there is obvious progressive, remarkable benefit.
2, the present invention is according to masson pine plantlet in vitro growth characteristics, determine the technical measures such as containers size, substrate composition, cuttings type, cuttings processing method, the proportioning of rooting nutrition solution adapting to Cuttage Seedling-Raising of Masson Pine, and nursery stock management after cuttage is described, whole technically reliable, simple to operate, nursery effect good, achievements conversion potentiality are large, and popularizing application prospect is wide.
3, the present invention with masson pine plantlet in vitro for mother plant for cutting carries out cuttage, overcome the problem that group training subculture cycle is long, seedling cost is higher on the one hand, breeding rate is high simultaneously, can be many for the qualified juvenile form fringe bar quantity of cuttage, very easily cuttage root-taking, Be very effective, has good economic benefit, social benefit and ecological benefits.
accompanying drawing identifies
Fig. 1 is masson pine plantlet in vitro mother plant for cutting in the present invention.
Fig. 2 is that in the present invention, masson pine takes root cuttage seeding.
Fig. 3 is the masson pine cutting survival seedling that the present invention obtains.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.1% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 65%.
Choose that newly to take out 3cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 10 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA50mg/L, 6-BA5mg/L, VB
150mg/L, VB
620mg/L, nicotinic acid 20mg/L, vitamin h 10mg/L, KNO
3850mg/L, KH
2pO
412h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 98.3%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 7d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 10d volumetric concentration 3 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 97.8%.
Embodiment 2:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.3% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 75%.
Choose that newly to take out 4cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.2% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 5 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA100mg/L, 6-BA10mg/L, VB
1100mg/L, VB
650mg/L, nicotinic acid 50mg/L, vitamin h 30mg/L, KNO
3850mg/L, KH
2pO
44h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 98.1%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 14d volumetric concentration 1 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 97.0%.
Embodiment 3:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.2% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 70%.
Choose that newly to take out 3cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 10 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA50mg/L, IBA50mg/L, ABT
150mg/L, 6-BA5mg/L, VB
150mg/L, VB
620mg/L, nicotinic acid 20mg/L, vitamin h 10mg/L, KNO
3850mg/L, KH
2pO
412h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-25 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 98%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-25 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 8d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 10d volumetric concentration 2 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 99.2%.
Embodiment 4:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.1% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 65%.
Choose that newly to take out 3cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.2% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 5 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA100mg/L, IBA50mg/L, ABT
150mg/L, 6-BA10mg/L, VB
1100mg/L, VB
650mg/L, nicotinic acid 50mg/L, vitamin h 30mg/L, KNO
3850mg/L, KH
2pO
44h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 25-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 98.2%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 25-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 12d volumetric concentration 2 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 97.5%.
Embodiment 5:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.2% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 70%.
Choose that newly to take out 4cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 10 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA50mg/L, IBA50mg/L, ABT
150mg/L, 6-BA10mg/L, VB
1100mg/L, VB
630mg/L, nicotinic acid 30mg/L, vitamin h 20mg/L, KNO
3850mg/L, KH
2pO
48h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 99.4%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 14d volumetric concentration 3 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 98.9%.
Embodiment 6:
A kind of masson pine plantlet in vitro spray single-node cutting seed-raising method, selects cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container.Mix obtain seedling medium with fermented-pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2, seedling medium is loaded the non-woven fabrics Seedling bag of 8cm × 9cm size specification, then seedling-cultivating tray is placed in, then the seedling medium installed to be drenched water, adopt the mixed liquor of volumetric concentration 0.3% carbendazim+volumetric concentration 0.5% potassium permanganate to for subsequent use after seedling medium sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is 75%.
Choose that newly to take out 3cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings; By the cuttings collected, in the treatment fluid that immersion volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.2% mancozeb+volumetric concentration 0.02% streptomycin is obtained by mixing rapidly, carry out the wash-out of cuttings and disinfect.In treatment fluid, soak time 5 minutes, then uses clear water cleaned standby seam.
The cuttings handled well is put into containing NAA75mg/L, IBA25mg/L, ABT
125mg/L, 6-BA10mg/L, VB
175mg/L, VB
640mg/L, nicotinic acid 40mg/L, vitamin h 20mg/L, KNO
3850mg/L, KH
2pO
410h is soaked in the rooting promoter of the mix preparation formation of 240mg/L, then use talcum powder rooting promoter furnishing pulpous state, directly insert in the seedling medium got ready after cuttings being dipped in slurry, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, root growth, obtain cuttage seeding of taking root, rooting rate is 98.6%.
Cuttage seeding of taking root is removed to nursery, land for growing field crops, and in the 1st month, note shading, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 9d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 12d volumetric concentration 3 ‰ 30N-13P-8K carries out Foliar Spraying; After 2 months, seedling-cultivating method carries out liquid manure and pest management routinely, and statistics Pinus massoniana Seedlings survival rate is 97.5%.
Claims (6)
1. a masson pine plantlet in vitro spray single-node cutting seed-raising method, it is characterized in that: comprise selection of container, matrix preparation, cuttings collection, cuttings process, cuttage and nursery bed management operation, select suitable container for plant growth, according to a certain ratio and after sterilization method gets out matrix, juvenile form spray is collected as cuttings and carries out wash-out and disinfect, after rooting promoter process, directly carry out cuttage, period adds the control of the fertile condition of high light warm water, obtains Pinus massoniana Seedlings after carrying out nursery bed management; Its operating procedure is as follows:
(1) selection of container: select cave size to be 6cm × 8cm, and 70 cave seedling-cultivating trays of side, cave band zigzag hole, as cuttage and seedling culture container;
(2) matrix prepares: non-woven fabrics Seedling bag seedling medium being loaded 8cm × 9cm size specification, then seedling-cultivating tray is placed in, water of then being drenched by the seedling medium installed, for subsequent use after sterilization, during cuttage, in Seedling bag, the water content control of seedling medium is at 65-75%;
(3) cuttings collection: choose that newly to take out 3-4cm then long, more than diameter 4mm, first lignification, the softness of top needle in the shape that clusters and the juvenile form masson pine spray flushed is cuttings;
(4) cuttings process: by the cuttings collected, immerses in treatment fluid rapidly, carries out the wash-out of cuttings and disinfect, soak time 5-10 minute, then using clear water cleaned standby seam;
(5) cuttage: the cuttings handled well is put into rooting promoter and soaks 4-12h, then use talcum powder rooting promoter furnishing pulpous state, in the seedling medium that after cuttings being dipped in slurry, directly inserting step (2) is got ready, by spraying and moisturizing, control seedling raising environment relative moisture 90-95%, temperature 20-30 DEG C, illumination 1500-2000lx, within about 1 month, cuttings top slightly extracts sprouting out, and root growth, obtains cuttage seeding of taking root;
(6) nursery bed management: cuttage seeding of taking root is removed to nursery, land for growing field crops, controls light temperature water and fertilizer condition well in 1-2 month, after 2 months, seedling-cultivating method carries out liquid manure and pest management routinely.
2. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, it is characterized in that: the raw material of the seedling medium described in step (2) comprises fermented-pine bark, coconut palm chaff, yellow soil and float stone, and pine bark, coconut palm chaff, yellow soil, float stone by volume=2: 2: 4: 2 mix and obtain seedling medium.
3. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the sterilization described in step (2) is the mixed liquor adopting volumetric concentration 0.1-0.3% carbendazim+volumetric concentration 0.5% potassium permanganate.
4. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the treatment fluid described in step (4) is the mixed solution of volumetric concentration 1.5% absolute ethyl alcohol+volumetric concentration 0.1% malonic acid+volumetric concentration 0.1-0.2% mancozeb+volumetric concentration 0.02% streptomycin.
5. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, is characterized in that: the rooting promoter described in step (5) is for containing: NAA50-100mg/L, IBA0-50mg/L, ABT
10-50mg/L, 6-BA5-10mg/L, VB
150-100mg/L, VB
620-50mg/L, nicotinic acid 20-50mg/L, vitamin h 10-30mg/L, KNO
3850mg/L, KH
2pO
4the mix preparation of 240mg/L.
6. masson pine plantlet in vitro spray single-node cutting seed-raising method according to claim 1, it is characterized in that: in the nursery bed management described in step (6), in 1st month, attention is shaded, illumination controls at 1500-2000lx, temperature at 20-30 DEG C, relative moisture 75-85%, the high phosphorus composite fertilizer every 7-10d volumetric concentration 1 ‰ 6N-35P-10K carries out Foliar Spraying; In 2nd month, note ventilation moisturizing, relative moisture 60-70%, the high-nitrogen compound fertilizer every 10-14d volumetric concentration 1-3 ‰ 30N-13P-8K carries out Foliar Spraying.
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| CN106212273A (en) * | 2016-07-15 | 2016-12-14 | 广西壮族自治区林业科学研究院 | Promote Pinus massoniana Lamb epicormic branch cuttage take root liquid fertilizer, preparation method and applications |
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| CN106212273A (en) * | 2016-07-15 | 2016-12-14 | 广西壮族自治区林业科学研究院 | Promote Pinus massoniana Lamb epicormic branch cuttage take root liquid fertilizer, preparation method and applications |
| CN106212273B (en) * | 2016-07-15 | 2018-03-13 | 广西壮族自治区林业科学研究院 | Promote masson pine epicormic branch cuttage take root liquid fertilizer, preparation method and applications |
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| CN109566622A (en) * | 2019-01-08 | 2019-04-05 | 广西壮族自治区林业科学研究院 | A kind of pine tree cuttage root-taking agent and its preparation method and application method |
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