CN103039366B - Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants - Google Patents

Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants Download PDF

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CN103039366B
CN103039366B CN201310018959.3A CN201310018959A CN103039366B CN 103039366 B CN103039366 B CN 103039366B CN 201310018959 A CN201310018959 A CN 201310018959A CN 103039366 B CN103039366 B CN 103039366B
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rhododendron
mycorrhizal
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azalea
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CN103039366A (en
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顾地周
杨丽娟
朱俊义
姜云天
徐洪伟
顾川岳
潘雨
禚玲玲
张学士
王秋爽
付航
倪伟佳
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Tonghua Normal University
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Abstract

The invention relates to the field of plant propagation, and particularly provides an industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants, which comprises the following steps: (1) treating branches of Changbai Mountain rhododendron plants, and taking the treated branches as explants for later use; (2) preparing a culture medium for enabling stems to take root and axillary buds to germinate and grow simultaneously: adding kinetin KT (0.03-0.010 mg.L<-1>), indolebutyric acid IBA (0.10-0.45 mg.L<-1>), naphthylacetic acid NAA (0.06-0.13 mg.L<-1>), gibberellin GA3 (1.40-2.60 mg.L<-1>), agar powder (7.8 g.L<-1>) and sucrose (10.0 g.L<-1>) into a basic culture medium, and regulating the pH value to 5.6; and culturing under the conditions that the illumination period is 9 h.d<-1>, the illumination intensity is 1000 lx and the temperature is 23+/-2 DEG C; (3) performing mycorrhizal treatment on tissue culture seedlings; and (4) culturing the tissue culture seedlings, and propagating to realize the industrial production of mycorrhizal seedlings of rhododendrons. According to the invention, the rhododendron plants can be subjected to mycorrhizal treatment at a high survival rate and can quickly propagate, thus finally realizing the industrial production of mycorrhizal seedlings of 9 rhododendrons on Changbai Mountain.

Description

A kind of batch production method of Rhododendron in Changbai platymiscium Mycorrhizal seedling
technical field
The present invention relates to a kind of plant propagation field, i.e. a kind of batch production method of Rhododendron in Changbai platymiscium Mycorrhizal seedling.
Background technology
In the prior art, Rhododendron in Changbai platymiscium comprise Rhododendron aureum georgi ( rhododendron chrysanthumpall.), short fruit cuckoo ( rhododendron brachycarpumd. Don), according to white cuckoo ( rhododendron micranthumturcz.), capitate rhododendron branchlet and leaf ( rhododendron parvifoliumadams.), large word cuckoo ( rhododendron schlippenbachiimaxim.), rhododendron dauricum ( rhododendron dauricuml.), korean rhododendron ( rhododendron mucronulatumturcz.), felt cuckoo ( rhododendron confertissimumnakai) and Rhododendron redowskianum ( rhododendron redowskianummaxim.) etc. have 9 kinds, be Jilin Province and lay special stress on protecting plant, wherein Rhododendron aureum georgi and Rhododendron redowskianum are the Precious And Rare Plants of China country three-level protective, and short fruit cuckoo is Changbaishan area Precious And Rare Plants.Wherein, Rhododendron aureum georgi, felt cuckoo, the white cuckoo of photograph and capitate rhododendron branchlet and leaf are evergreen shrubss, and short fruit cuckoo is evergreen dungarunga, and rhododendron dauricum is half evergreen shrubs.Rhododendron aureum georgi, Rhododendron redowskianum and felt cuckoo distribution height above sea level reach 2500 meters high, belong to alpine rose.Rhododendron aureum georgi, large word cuckoo, higher according to white cuckoo, capitate rhododendron branchlet and leaf, korean rhododendron and rhododendron dauricum complete stool volatile oil content are essential oil and medicinal plant.These 9 kinds of cuckoos can be all garden plants by artificial introduction and acclimatization, have important society, economy and the ecological value.
Above 9 kinds of cuckoos are to water and soil conservation and maintain the ecological balance and play an important role, and are the germ plasm resource of azalea breeding.Dan Qi China distributes very narrow, only on Changbai Mountain, has larger population to distribute, and other area is only fragmentary distribution, and because people disorderly adopt disorderly and dig, wild resource suffers great threat.These 9 kinds of Rhododendron seeds are tiny, and germination rate is low, and the seedling survival rate forming after sprouting is extremely low; Cottage propagation needs a large amount of Rhododendron simsii Planch branches, wild resource is destroyed greatly, and rooting rate and transplanting survival rate extremely low; And utilize tissue culture technique to breed, and thering is the advantages such as to produce test-tube plantlet amount per year large, and speed is fast, but still have the problems such as acclimatization and transplants survival rate is low, poor growth, this just causes applying being subject to certain restrictions.
Through lot of experiments, find, azalea root system does not have root hair, and absorbing the nutrient component energy force rates such as moisture, macroelement, trace element and organic substance, to have the root system of root hair much smaller.But under natural conditions, azalea radicula has the parasitism of azalea class EM (Eriocdimyochrriaz, EM) endotrophic mycorrhiza fungi, thus overcome that cuckoo causes because root system is undeveloped to absorption of nutrient ingredients difficulty.Rhododendron Mycorrhizal is processed and is conducive to improve survival rate.
Summary of the invention
The object of the invention is to provide for above-mentioned deficiency a kind of rhododendron Mycorrhizal is processed, realize the Fast-propagation of Mycorrhizal seedling, the batch production method of the Rhododendron in Changbai platymiscium Mycorrhizal seedling that survival rate is high.
Technical solution of the present invention is: a kind of batch production method of Rhododendron in Changbai platymiscium Mycorrhizal seedling, is characterized in that step is as follows:
(1) get Rhododendron in Changbai platymiscium branch, short its sprouting of lateral bud growth of water planting, cuts processing by fresh and tender lateral bud, cuts into 1 section, 1 leaf standby as explant.
(2) medium (stem section take root axillary bud sprouting growth medium): 87 mgL simultaneously -1(NH 4) 2sO 4, 375 mgL -1nH 4nO 3, 180 mgL -1kNO 3, 320 mgL -1caCl 22H 2o, 245 mgL -1mgSO 47H 2o, 436 mgL -1kH 2pO 4; 18.4 mgL -1feSO 47H 2o, 24.9 mgL -1na 2eDTA2H 2o; 12.4 mgL -1mnSO 44H 2o, 7.2 mgL -1znSO 47H 2o, 5.7 mgL -1h 3bO 3, 0.32 mgL -1kI, 0.05 mgL -1na 2moO 42H 2o; Medium supplemented kinetin KT (0.03～0.010 mgL -1), indolebutyric acid IBA (0.10～0.45 mgL -1), methyl α-naphthyl acetate NAA (0.06～0.13 mgL -1), gibberellin GA 3(1.40～2.60 mgL -1), agar powder 7.8 gL -1, add sucrose 10.0 gL -1, regulate pH value to 5.6; Periodicity of illumination 9 hd -1, cultivate under intensity of illumination 1000 lx and temperature (23 ± 2) ℃ condition.
(3) Mycorrhizal of test-tube plantlet: test-tube plantlet is taken out from blake bottle, clean root agar with clear water, then put into bacterium liquid and soak 18～24 h; Described bacterium fluid component be configured to: the composition of 1000 mL medium and content are: KH 2pO 4380 mg, MgSO 47H 2o149 mg, Catergen 6 mg, calcium pantothenate 67 mg, nicotinic acid 1.4 mg, peptone 3.0 g, glucose 16.5 g; By carrying out Mixed culture in the triangular pyramidal bottle that contains six bacterial classification access liquid-based basal culture mediums, obtain liquid spawn mother liquor; Again by liquid spawn mother liquor 500 mL, after brown sugar 500 g dissolve, add water to after 50 L fermentation 7～10 d bacterium liquid; Six kinds of main ericoid mycorrhizal fungis are that 9 kinds of wild-type azalea section plant root separation and purification obtain from Changbai Mountain, are respectively phialocephala fortinii, pezizilla ericace, oidiodendron maius, meliniomyces variabilis, cryptosporiopsis ercae, acaulospora lacunosa.
(4) cultivation of Mycorrhizal seedling: the test-tube plantlet after step (3) Mycorrhizal is planted in the matrix of having filled with bacterium liquid, matrix is humus soil, pine needle and vermiculite become thoroughly decomposed, ratio is 3:2:1, cover the good plastic film heat and moisture preserving of light transmission, start temperature and be controlled at (25 ± 2) ℃, after 15 d, temperature is controlled at (20 ± 2) ℃, keeps relative moisture between 70%～75%, 10 hd -1natural lighting; Expand numerously, realize the batch production of azalea Mycorrhizal seedling and produce.
Described rhododendron is Rhododendron aureum georgi, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.13～0.14 mgL -1)+IBA (0.05～0.08 mgL -1)+GA 3(2.70～2.90 mgL -1).
Described rhododendron is short fruit cuckoo, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.09～0.12 mgL -1)+IBA (0.03～0.07 mgL -1)+GA 3(2.80～3.00 mgL -1).
Described rhododendron is for according to white cuckoo, and the take root medium of axillary bud sprouting growth simultaneously of stem section is: medium+IAA (0.20～0.25 mgL -1)+NAA (0.02～0.05 mgL -1)+GA 3(2.30～2.60 mgL -1).
Described rhododendron is capitate rhododendron branchlet and leaf, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.08～0.12 mgL -1)+IBA (0.15～0.20 mgL -1)+GA 3(2.00～2.30 mgL -1)
Described rhododendron is large word cuckoo, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.07～0.10 mgL -1)+NAA (0.01～0.03 mgL -1)+GA 3(2.20～2.50 mgL -1).
Described rhododendron is rhododendron dauricum, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+NAA (0.02～0.04 mgL -1)+GA 3(1.00～1.50 mgL -1).
Described rhododendron is korean rhododendron, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.03～0.06 mgL -1)+NAA (0.03～0.07 mgL -1)+GA 3(0.09～1.30 mgL -1).
Described rhododendron is felt cuckoo, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+KT (0.08～0.12 mgL -1)+NAA (0.05～0.07 mgL -1)+GA 3(1.80～2.00 mgL -1).
Described rhododendron is Rhododendron redowskianum, and the take root medium of simultaneously axillary bud sprouting growth of stem section is: medium+IAA (0.14～0.18 mgL -1)+GA 3(1.30～1.80 mgL -1).
The present invention combines the group culturation rapid propagating technology of azalea and group cultivation nursery garden rooting technology, carries out the Fast-propagation of azalea Mycorrhizal seedling.Utilize a kind of medium to complete that the tender stem section of azalea is taken root in vitro simultaneously and axillary bud sprouting growth after, when azalea test tube seedling and propagating grows to certain altitude to needs quantity and height of seedling, test-tube plantlet is taken out from blake bottle, put into the mixed-matrix of plant after homemade bacterium liquid soaks turfy soil, rot pine needle and river sand, under natural lighting, moisture-heat preservation can complete the Fast-propagation of azalea Mycorrhizal seedling, and improves survival rate.
Advantage of the present invention is: the parasitism of endotrophic mycorrhiza fungi has improved nutrition status of the plant, regulate metabolic activity, improve utilization rate of fertilizer, strengthen plant resistance, can add and promote growing of azalea, improve transplanted seedling survival rate, save production cost, increase economic benefit, realize the batch production of the 9 kinds of azalea Mycorrhizal seedlings in Changbai Mountain and produce.
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.
Embodiment
A batch production method for Rhododendron in Changbai platymiscium Mycorrhizal seedling, step is as follows:
1 material and processing
In Changbaishan area, gather Rhododendron aureum georgi ( rhododendron chrysanthumpall.), short fruit cuckoo ( rhododendron brachycarpumd. Don), according to white cuckoo ( rhododendron micranthumturcz.), capitate rhododendron branchlet and leaf ( rhododendron parvifoliumadams.), large word cuckoo ( rhododendron schlippenbachiimaxim.), rhododendron dauricum ( rhododendron dauricuml.), korean rhododendron ( rhododendron mucronulatumturcz.), felt cuckoo ( rhododendron confertissimumnakai) and Rhododendron redowskianum ( rhododendron redowskianumthe azalea branch of 9 kinds such as Maxim.), and branch terminal bud is removed to short its sprouting of lateral bud growth of water planting in the Gibberellins solution of 500 times of liquid.When sprouting of lateral bud grows to 2.50～3.00 cm, fresh and tender lateral bud is cut, remove tender leaf and petiole, with 70% ethanol (volume fraction), rinse and wash 30 s, move to and in saturated liquor natrii hypochloritis, soak 10 min, aseptic water washing 8 times, aseptic filter paper blots surface moisture, removes that after the tissue of ethanol and clorox poison wound, to be cut into 1 section, 1 leaf standby as explant.
2 methods
The Fast-propagation of 2.1 9 kinds of azaleas
Medium component and content: 87 mgL -1(NH 4) 2sO 4, 375 mgL -1nH 4nO 3, 180 mgL -1kNO 3, 320 mgL -1caCl 22H 2o, 245 mgL -1mgSO 47H 2o, 436 mgL -1kH 2pO 4; 18.4 mgL -1feSO 47H 2o, 24.9 mgL -1na 2eDTA2H 2o; 12.4 mgL -1mnSO 44H 2o, 7.2 mgL -1znSO 47H 2o, 5.7 mgL -1h 3bO 3, 0.32 mgL -1kI, 0.05 mgL -1na 2moO 42H 2o.Medium supplemented kinetin KT (0.03～0.010 mgL -1), indolebutyric acid IBA (0.10～0.45 mgL -1), methyl α-naphthyl acetate NAA (0.06～0.13 mgL -1) and gibberellin GA 3(1.40～2.60 mgL -1), agar powder 7.8 gL -1, add sucrose 10.0 gL -1, regulating pH value to 5.6,9 kinds of tender stem sections of azalea are at periodicity of illumination 9 hd -1, cultivate under intensity of illumination 1000 lx and temperature (23 ± 2) ℃ condition.And then screen and determine that 9 kinds of cuckoo scape sections are taken root and kinetin, indolebutyric acid, methyl α-naphthyl acetate and the gibberellin best concentration ratio of axillary bud sprouting growth.
Until 9 kinds of cuckoo scape sections, take root and axillary bud sprouting while growing to 2.00～3.50 cm, when axillalry bud sends 5～7 leaves, stay 1 leaf to cut seedling axillalry bud dry, cut into after 1 section, 1 leaf seedling is dry again, again little stem section is transferred to corresponding stem section is taken root and axillary bud sprouting growth medium in organize and trains the numerous soon of seedling, to obtain a large amount of azalea plantlets.
2.2 bacterium fluid component and configurations
Minimal medium composition and content (content of 1000 mL medium) are: KH 2pO 4380 mg, MgSO 47H 2o149 mg, Catergen 6 mg, calcium pantothenate 67 mg, nicotinic acid 1.4 mg, peptone 3.0 g, glucose 16.5 g.
From Changbai Mountain, the sociales of 6 kinds of main ericoid mycorrhizal fungis of 9 kinds of wild-type azalea section plant root separation and purification acquisitions, are respectively phialocephala fortinii, pezizilla ericace, oidiodendron maius, meliniomyces variabilis, cryptosporiopsis ercae, acaulospora lacunosa.
By containing 6 bacterial classifications, in the triangular pyramidal bottle of liquid-based basal culture medium, carry out Mixed culture, obtain liquid spawn mother liquor, then by liquid spawn mother liquor 500 mL, after brown sugar 500 g dissolve, add water to after 50 L fermentation 7～10 d bacterium liquid.
Mycorrhizal and the cultivation of the group training seedling of 2.3 9 kinds of azaleas
Utilize 9 kinds of tender stem sections of azalea to complete that stem section is taken root in vitro simultaneously and axillary bud sprouting growth after, until azalea test tube seedling and propagating, to needs quantity and height of seedling, grow to 2.50 cm when above, test-tube plantlet is taken out from blake bottle, with clear water, clean root agar, then (bacterium liquid contains to put into the immersion of bacterium liquid phialocephala fortinii, pezizilla ericace, oidiodendron maius, meliniomyces variabilis, cryptosporiopsis ercae, acaulospora lacunose6 kinds of ericoid mycorrhizal fungis, all equal proportion.In other composition 1000mL, contain KH 2pO 4380 mg, MgSO 47H 2o149 mg, Catergen 6 mg, calcium pantothenate 67 mg, nicotinic acid 1.4 mg, peptone 3.0 g, glucose 16.5 g, agar powder 8.5 g, 1.5% deoxycholic acid sodium solution 4 mL and 0.05 mg heteroauxin), take out and plant in the matrix (matrix is humus soil, pine needle and vermiculite become thoroughly decomposed) of having filled with bacterium liquid, cover the good plastic film heat and moisture preserving of light transmission, be placed under natural lighting condition, when humidity temperature at too high or noon raises, film opened to the osculum ventilation of taking a breath, when humidity reduces, can spray in the morning proper amount of clear water.
3 ?result
The take root medium of simultaneously axillary bud sprouting growth of 3.1 9 kinds, Changbai Mountain cuckoo stem sections
The take root medium of simultaneously axillary bud sprouting growth of Rhododendron aureum georgi stem section is: medium+KT (0.13～0.14 mgL -1)+IBA (0.05～0.08 mgL -1)+GA 3(2.70～2.90 mgL -1).The average rooting rate 99.9% of tender stem section, the average growth length after axillary bud sprouting is 3.06 cm.
The take root medium of simultaneously axillary bud sprouting growth of short fruit cuckoo stem section is: medium+KT (0.09～0.12 mgL -1)+IBA (0.03～0.07 mgL -1)+GA 3(2.80～3.00 mgL -1).The average rooting rate 99.7% of tender stem section, the average growth length after axillary bud sprouting is 3.17 cm.
According to the take root medium of axillary bud sprouting growth simultaneously of white cuckoo stem section, be: medium+IAA (0.20～0.25 mgL -1)+NAA (0.02～0.05 mgL -1)+GA 3(2.30～2.60 mgL -1).The average rooting rate 99.4% of tender stem section, the average growth length after axillary bud sprouting is 3.22 cm.
The take root medium of simultaneously axillary bud sprouting growth of capitate rhododendron branchlet and leaf stem section is: medium+KT (0.08～0.12 mgL -1)+IBA (0.15～0.20 mgL -1)+GA 3(2.00～2.30 mgL -1).The average rooting rate 99.5% of tender stem section, the average growth length after axillary bud sprouting is 2.96 cm.
The take root medium of axillary bud sprouting growth simultaneously of large word cuckoo stem section is: medium+KT (0.07～0.10 mgL -1)+NAA (0.01～0.03 mgL -1)+GA 3(2.20～2.50 mgL -1).The average rooting rate 99.9% of tender stem section, the average growth length after axillary bud sprouting is 2.87 cm.
The take root medium of simultaneously axillary bud sprouting growth of rhododendron dauricum stem section is: medium+NAA (0.02～0.04 mgL -1)+GA 3(1.00～1.50 mgL -1).The average rooting rate 99.5% of tender stem section, the average growth length after axillary bud sprouting is 3.30 cm.
The take root medium of simultaneously axillary bud sprouting growth of korean rhododendron stem section is: medium+KT (0.03～0.06 mgL -1)+NAA (0.03～0.07 mgL -1)+GA 3(0.09～1.30 mgL -1).The average rooting rate 99.0% of tender stem section, the average growth length after axillary bud sprouting is 3.37 cm.
The take root medium of simultaneously axillary bud sprouting growth of felt cuckoo stem section is: medium+KT (0.08～0.12 mgL -1)+NAA (0.05～0.07 mgL -1)+GA 3(1.80～2.00 mgL -1).The average rooting rate 99.6% of tender stem section, the average growth length after axillary bud sprouting is 2.80 cm.
The take root medium of simultaneously axillary bud sprouting growth of Rhododendron redowskianum stem section is: medium+IAA (0.14～0.18 mgL -1)+GA 3(1.30～1.80 mgL -1).The average rooting rate 99.8% of tender stem section, the average growth length after axillary bud sprouting is 2.77 cm.
Fast-propagation, hardening and the transplanting of 3.2 9 kinds of azalea Mycorrhizal seedlings
By 2.1, method in 2.2 and 2.3, when 9 kinds of azalea seeding propagations are after needs quantity, the healthy and strong plantlet of all choosing more than 2.50～3.00 cm is cleaned after root agar, plantlet is put into bacterium liquid and soak 18～24 h, taking-up is planted, and (matrix is humus soil to the matrix of having filled with bacterium liquid, pine needle and vermiculite become thoroughly decomposed, ratio is 3:2:1) in, cover the good plastic film heat and moisture preserving of light transmission, start temperature and be controlled at (25 ± 2) ℃, be beneficial to all and the growth of fungi and building together of indian azalea root, after 15 d, temperature is controlled at (20 ± 2) ℃, keep relative moisture between 70%～75%, 10 hd -1natural lighting, when humidity temperature at too high or noon raises, film is opened to the osculum ventilation of take a breath, during humidity reduction, can spray in the morning proper amount of clear water, survival rate all reaches more than 97.8%, and growing way is extremely strong.
Average by 12 stem sections of every bottle graft kind, it within average 28 days, is 1 cycle, each stem section on average can on average cut into 7 sections of calculating in 1 cultivation cycle, has every year n the cycle (9 kinds of cuckoos are 12 cycles every year) to expand numerous, and a year production cuckoo mycorhiza flower seedling number is: ∑ is produced Va Mycorrhiza Seedling=12 * 7 per year n* 97.8% strain, visible the present invention can meet the batch production of the 9 kinds of azalea Mycorrhizal seedlings in Changbai Mountain and produce.

Claims (1)

1. a batch production method for Rhododendron in Changbai platymiscium Mycorrhizal seedling, is characterized in that step is as follows:
(1) get Rhododendron in Changbai platymiscium branch, short its sprouting of lateral bud growth of water planting, cuts processing by fresh and tender lateral bud, cuts into 1 section, 1 leaf standby as explant;
(2) medium: 87 mgL -1(NH 4) 2sO 4, 375 mgL -1nH 4nO 3, 180 mgL -1kNO 3, 320 mgL -1caCl 22H 2o, 245 mgL -1mgSO 47H 2o, 436 mgL -1kH 2pO 4; 18.4 mgL -1feSO 47H 2o, 24.9 mgL -1na 2eDTA2H 2o; 12.4 mgL -1mnSO 44H 2o, 7.2 mgL -1znSO 47H 2o, 5.7 mgL -1h 3bO 3, 0.32 mgL -1kI, 0.05 mgL -1na 2moO 42H 2o; Medium supplemented kinetin KT 0.03～0.010 mgL -1, indolebutyric acid IBA 0.10～0.45 mgL -1, methyl α-naphthyl acetate NAA 0.06～0.13 mgL -1, gibberellin GA 31.40～2.60 mgL -1, agar powder 7.8 gL -1, add sucrose 10.0 gL -1, regulate pH value to 5.6; Periodicity of illumination 9 hd -1, cultivate under intensity of illumination 1000 lx and 23 ± 2 ℃ of conditions of temperature;
(3) Mycorrhizal of test-tube plantlet: test-tube plantlet is taken out from blake bottle, clean root agar with clear water, then put into bacterium liquid and soak 18～24 h; Described bacterium fluid component be configured to: the composition of 1000 mL minimal mediums and content are KH 2pO 4380 mg, MgSO 47H 2o149 mg, Catergen 6 mg, calcium pantothenate 67 mg, nicotinic acid 1.4 mg, peptone 3.0 g, glucose 16.5 g; Six bacterial classifications are poured in the triangular pyramidal bottle that contains minimal medium liquid and are carried out Mixed culture, obtain liquid spawn mother liquor, then by liquid spawn mother liquor 500 mL, after brown sugar 500 g dissolve, add water to after 50 L fermentation 7～10 d bacterium liquid; Six kinds of azalea class bacterial classifications are that 9 kinds of wild-type azalea section plant root separation and purification obtain from Changbai Mountain, are respectively phialocephala fortinii, pezizilla ericace, oidiodendron maius, meliniomyces variabilis, cryptosporiopsis ercae, acaulospora lacunosa;
(4) cultivation of Mycorrhizal seedling: the test-tube plantlet after step (3) Mycorrhizal is planted in the matrix of having filled with bacterium liquid, matrix is humus soil, pine needle and vermiculite become thoroughly decomposed, ratio is 3:2:1, cover the good plastic film heat and moisture preserving of light transmission, start temperature and be controlled at 25 ± 2 ℃, after 15 d, temperature is controlled at 20 ± 2 ℃, keeps relative moisture between 70%～75%, 10 hd -1natural lighting; Expand numerously, realize the batch production of azalea Mycorrhizal seedling and produce.
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CN105075632A (en) * 2015-09-01 2015-11-25 大理苍山植物园生物科技有限公司 Rhododendron decorum aseptic seedling mycorrhization technology
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CN109618932B (en) * 2019-01-11 2021-06-18 东北林业大学 Method for inducing adventitious buds of rhododendron dauricum and regenerating plants

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1630711A1 (en) * 1989-03-06 1991-02-28 Латвийский Государственный Университет Им.П.Стучки Method of nutrient substrate preparation for plant tissues of rhododendrons and azaleas cultivation
JP2970277B2 (en) * 1992-12-25 1999-11-02 王子製紙株式会社 A method for promoting rooting of cultured shoots in Rhododendron plants
CN1732759A (en) * 2005-08-17 2006-02-15 中国林业科学研究院林业研究所 Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz.
CN101138322A (en) * 2007-08-09 2008-03-12 贵州省生物技术研究所 Cremastra appendiculata tissue culturing rhizome fungus root seedling raising technique
CN101935616A (en) * 2009-12-16 2011-01-05 上海市园林科学研究所 Rhododendron mycorrhizal fungi bacterial strain, culture medium and culture method thereof
CN102703333A (en) * 2012-06-25 2012-10-03 上海市园林科学研究所 Mycorrhizal fungi strain efficiently growing with rhododendron seedling

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1630711A1 (en) * 1989-03-06 1991-02-28 Латвийский Государственный Университет Им.П.Стучки Method of nutrient substrate preparation for plant tissues of rhododendrons and azaleas cultivation
JP2970277B2 (en) * 1992-12-25 1999-11-02 王子製紙株式会社 A method for promoting rooting of cultured shoots in Rhododendron plants
CN1732759A (en) * 2005-08-17 2006-02-15 中国林业科学研究院林业研究所 Tissue culturing, rapid propagating and transplanting method of Rhododendron mucronulatum Turcz.
CN101138322A (en) * 2007-08-09 2008-03-12 贵州省生物技术研究所 Cremastra appendiculata tissue culturing rhizome fungus root seedling raising technique
CN101935616A (en) * 2009-12-16 2011-01-05 上海市园林科学研究所 Rhododendron mycorrhizal fungi bacterial strain, culture medium and culture method thereof
CN102703333A (en) * 2012-06-25 2012-10-03 上海市园林科学研究所 Mycorrhizal fungi strain efficiently growing with rhododendron seedling

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
JP第2970277号B2 1999.11.02
Martin Vohník et al..The inoculation with Oidiodendron maius and Phialocephala fortinii alters phosphorus and nitrogen uptake, foliar C:N ratio and root biomass distribution in Rhododendron cv. Azurro.《SYMBIOSIS》.2005,(第40期),摘要，第88页"Fungal Compatibility Experiment"部分.
The inoculation with Oidiodendron maius and Phialocephala fortinii alters phosphorus and nitrogen uptake, foliar C:N ratio and root biomass distribution in Rhododendron cv. Azurro;Martin Vohník et al.;《SYMBIOSIS》;20051231(第40期);摘要，第88页"Fungal Compatibility Experiment"部分 *
刘振华.杜鹃花菌根真菌分离鉴定及多样性分析.《中国林业科学研究院硕士学位论文》.2011,摘要第3点，正文16页第1.3.1.4节，正文第11页倒数第1行-第12页第4行.
基于均匀设计法优化照白杜鹃高效快繁体系;顾地周等;《林业实用技术》;20081231(第12期);第1.1、1.2、2.1、2.3节 *
杜鹃花菌根真菌分离鉴定及多样性分析;刘振华;《中国林业科学研究院硕士学位论文》;20110331;摘要第3点，正文16页第1.3.1.4节，正文第11页倒数第1行-第12页第4行 *
顾地周等.基于均匀设计法优化照白杜鹃高效快繁体系.《林业实用技术》.2008,(第12期),第1.1、1.2、2.1、2.3节.

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