CN102498899B - Method for cuttage propagation of rhododendron lapponicum - Google Patents
Method for cuttage propagation of rhododendron lapponicum Download PDFInfo
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Abstract
The invention relates to a method for cuttage propagation of rhododendron lapponicum, which includes: making a mixture of peat and pearlite into cuttage substrate according to a volute ratio of from 1:3 to 1:4, cutting a stem of a current year growing semi-lignified branch of a rhododendron lapponicum plant which is under no pest and disease damage and 3-5 years old as a cutting, and retaining two to three complete leaves at the top of the cutting; dipping the base of the cutting into rooting reagent A or B prior to inserting into the substrate; and performing intermittent spray for subsequent water management, maintaining mass water content of soil to be 60-65%, and maintaining air humidity to be more than 80% during a period from cuttage to rooting of a cutting seedling. The rooting reagent A includes IBA (indole-3-butyric acid) 0.8-1.0% and thiophanate-methyl 2-4%. The rooting reagent B includes IBA 0.015-0.02% and thiophanate-methyl 1-2%. By the method, rooting rate can reach morethan 85%, rooting of the cutting only requires about 60 days, and production cycle is short.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to the cuttage breeding method of alpine rose.
Background technology
Azalea is universally acknowledged famous and precious ornamental flower, also is one of China's ten large famous flowers.Alpine rose be the Ericaceae Rhododendron (
Rhododendron)In a large class, generally refer to without squama azalea subgenus, the monkeyhead rhododendron leaf in squama azalea subgenus, the root of Egg-shaped Leaves Rhododendron subgenus arranged, and their Hybrid.Alpine rose mainly is distributed in cheuch broad leaved forest or the mountain top of China Midwest height above sea level 1400～4500m and fills with in the thick grass, its corolla is very large, color abundant, the flower appearance is graceful, plant type is beautiful, the four seasons are evergreen, the low temperature of ability-26 ℃, be not only and enjoy the annual flower of making a pet of, or the good kind that township afforestation beautifies has vast potential for future development.Yet the alpine rose potted flower on the China market more than 90% is basically all from external import, and the alpine rose seedling that domestic enterprise provides is compared with import seed, and quality gap is larger, is difficult to and external competition.
The seed of alpine rose is very little, not only sows difficulty and seedling growth is very different, very easily produces variation, is not suitable for large-scale breeding.Although tissue cultivate have reproduction coefficient high, can effectively keep the advantage such as maternal plant merit, its cost is high, the cycle long, the hardening survival rate is low, has limited the Rapid Popularization of improved seeds.Cottage propagation is the most frequently used a kind of method of azalea breeding, belgian azalea and similar type thereof are all take cottage propagation as main, but the cottage propagation difficulty of alpine rose is large, and conventional cottage propagation rooting rate is extremely low or do not take root, and can't satisfy the growing market demand.In great shortage for domestic alpine rose seedling, the tissue culture propagation cost is high, is not suitable with the present situation of improved seeds fast breeding, Effective Raise alpine rose reproductive survival rate how, shorten growing-seedling period, reduce production costs, become problem demanding prompt solution in the alpine rose industrialized development.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the defective that cuttage with existing cuttage breeding method cottage propagation alpine rose is difficult for taking root, rooting rate is low, its objective is the cuttage breeding method that a kind of easy and simple to handle, with low cost, alpine rose that rooting rate is high is provided.The present invention also provides root-growing agent and the using method thereof of the cottage propagation of suitable alpine rose.
For solving the problems of the technologies described above, the cuttage breeding method of alpine rose provided by the present invention may further comprise the steps:
(1) prepare in the seedbed: build shed in the seedbed, plastic covering film on shed, laying depth is the cutting medium of 12～15cm on the seedbed, below cutting medium, lay the ground hot line, cutting medium is peat and perlitic mixture, peat and perlitic volume ratio are 1:3～1:4, water after the matrix on the seedbed is smooth permeablely, use the liquor natrii hypochloritis of 1000ppm to ground sterilization in seedbed and the shed again;
(2) transplant a cutting to select: gathers life in 3～5 years without the alpine rose plant of the damage by disease and insect maternal material as cutting, 9～October, the semi-lignified branch that clip is given birth to then from this female parent material is for transplanting a cutting material, be that the stem section of 7～8cm is for transplanting a cutting with transplanting a cutting that material cuts, node more than 2 is arranged in the described cutting, and the top keeps 2～3 intact leafs;
(3) processing of taking root before the cuttage: the base portion of will transplanting a cutting dips in natural air drying behind root-growing agent A 10s～20s, and the base portion of maybe will transplanting a cutting is immersed among the root-growing agent B natural air drying behind 24h～36h; The prescription of described root-growing agent A is 0.8～1.0%IBA+2～4% thiophanate methyl, and the prescription of described root-growing agent B is 0.015～0.02% IBA+1～2% thiophanate methyl; Thiophanate methyl among root-growing agent A and the root-growing agent B is matching while using; Described percentage is mass fraction;
(4) cuttage: 5～6cm in the described matrix of cutting inserting step (1) that will process with root-growing agent makes to distance and transplants a cutting the 1st blade of base portion from stromal surface 2cm ± 0.3cm;
(5) manage after the cuttage:
A. water supply in media and ambient humidity management: after cuttage, immediately matrix spraying is watered permeable, and adopt the intermittent spraying mode to carry out follow-up water management, the mass water content of soil of matrix is 60～65%, from the beginning cuttage to the long root of cuttage seeding, keeps air humidity more than 80%;
B. illumination and temperature treatment: adopt the natural daylight full exposure to process after the cuttage, the temperature on daytime is controlled at 18～25 ℃, and the temperature at night is controlled at 12～18 ℃, and the substrate temperature whole day remains on 18～22 ℃;
C. the extermination of disease and insect pest: after the cuttage, adopting mass fraction is 1000～1500 times of liquid sprayings of thiophanate methyl cuttage seeding of 50%, sprays once every 10～15 days, and in time removes the cutting of catching an illness;
D. nutrient management: before the long root of cutting, sprayed one time 0.1～0.5% urea every 5～15 days, after cutting sends out roots, spray once every 10～15 days potassium dihydrogen phosphates with 0.1～0.5% urea and 0.1～0.2%; When cuttage seeding formation diameter is the root ball of 1.5～3cm, transplant to the seedling alms bowl; Described percentage is mass fraction.
The branch of the medium growth rate of clip and medium-coarse is taken root easily than eugonic branch, transplant a cutting the loss of water of material for making cutting more easily take root and reduce, all right, the first day at step (2) clip cutting material waters for maternal material, and 8 o'clock to 14 o'clock next day, from this female parent material clip give birth to then diameter at the semi-lignified branch of 0.5～0.8cm for transplanting a cutting material.
In step (2) lower end of described cutting is cut into flat mouth, to reduce the formation of callus.
Comprise that also automatic spraying system is installed to carry out after step (5) cuttage management A. water supply in media and ambient humidity and manage described spraying and water permeable and the intermittent spraying mode is carried out follow-up water management.
Can also after step (5) cuttage, manage in the C. extermination of disease and insect pest and the D. nutrient management process with automatic spraying system and spray thiophanate methyl, urea and dihydrogen phosphate solution.
It is that fine day, half cloudy half fine, overcast and rainy 3 kinds of patterns are set that the intermittent spraying mode of described automatic spraying system is carried out follow-up water management, and air humidity is more than 80% in the maintenance shed; The taking root early stage of the 1st～4 week after cuttage, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 10 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 15 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; During the taking root of the 5th～10 week, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 12 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 20 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; Before the 11st thoughtful transplanting, spraying system 10:00 opens, and 17:00 closes, fine spray intervals 60 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 180 minutes, 30 seconds duration of spraying; Overcast and rainyly do not spray, keeping the mass water content of soil of matrix is 60～65%, avoids the blade face dry.
Described fine day, half cloudy half finely, overcast and rainy is as the criterion with weather forecast.
Described natural daylight full exposure is processed and is referred to utilize the natural lighting of whole day to cultivate cuttage seeding, cuttage seeding is not carried out the measure of any shading.
The root-growing agent A of alpine rose cottage propagation provided by the present invention is by forming in 0.8～1.0%IBA+2 of mass fraction～4% thiophanate methyl.
The root-growing agent B of alpine rose cottage propagation provided by the present invention is by in 0.015～0.02% IBA+1 of mass fraction～2% thiophanate methyl.
Described IBA is the 3-indolebutyric acid; The chemical name of thiophanate methyl is 1,2-two (3-carbometoxyl-2-ghiourea group) benzene.
Thiophanate methyl matching while using when the using method of the root-growing agent A of described alpine rose cottage propagation is use among the root-growing agent A; Alpine rose is transplanted a cutting base portion dip in root-growing agent A 10s～20s, insert again matrix behind the natural air drying.
Thiophanate methyl matching while using when the using method of the root-growing agent B of described alpine rose cottage propagation is use among the root-growing agent B; Alpine rose is transplanted a cutting base portion be immersed in 24h～36h among the root-growing agent B, insert again matrix behind the natural air drying.
The present invention has following advantage with respect to prior art:
1, the present invention is by selecting the aggregate measures such as suitable matrix, suitable root-growing agent and processing mode and suitable fertilising, temp. and humidity, effectively solved the difficult problem that the alpine rose cuttage is difficult for taking root, the cottage propagation rate is low, effectively increase rooting rate and quality of rooting, rooting rate can reach more than 85%, is one of approach of the effective breeding of alpine rose.
2, the inventive method has promoted the cutting Rapid Rooting, and rooting of cuttings only needed about 60 days, and is with short production cycle, with low cost.
3, the inventive method is fully suitable adopts auto spraying water and fertilizer management system and the operation of temp. and humidity automatic control system, and method of operating is simple, be the effective modernization method of alpine rose scale cuttage Fast-propagation.
Embodiment
The present invention is described in further detail below in conjunction with embodiment, but they are not limitation of the invention.Used test material alpine rose kind among the embodiment
Rhododendron' Nova Zembla ', alpine rose kind
Rhododendron' Furnlvall daughter ', automatic spraying system, ground hot line, peat, perlite, each reagent of root-growing agent etc. all can buy from the commercial channel and obtain.
Embodiment 1
Test material: alpine rose kind
Rhododendron' Nova Zembla '.
(1) prepare in the seedbed: setting up width in the breeding spot of choosing is 1m, and length is the seedbed of 15m, builds shed in the seedbed, plastic covering film on shed
,Laying depth is the cutting medium of 12～15cm on the seedbed, cutting medium with peat and perlite by volume 1:3 mix, below cutting medium, lay the ground hot line, after stromal surface is smooth, start spraying system, make water content of substrate saturated, use again the liquor natrii hypochloritis of 1000ppm to ground sterilization in seedbed and the shed; The used automatic spraying system of floral nursery is installed in the shed;
(2) cutting is selected: the alpine rose kind without damage by disease and insect of choosing seedling age life in 3 years
Rhododendron' Nova Zembla' is as female parent, in point in the mornings 9 September 14, from this female parent material clip give birth to then diameter at the semi-lignified branch of 0.5～0.8cm for transplanting a cutting material.Cut be the stem section of 7～8cm for transplanting a cutting, the top keeps 3 intact leafs, can keep terminal bud, transplanting a cutting has 2 nodes, the lower end is cut into flat mouth.
(3) processing of taking root before the cuttage: will transplant a cutting take 15 as a bundle, the base portion alignment is dipped in 10s in root-growing agent A, cuttage is in the described matrix of step (1) behind the natural air drying, the cuttage degree of depth is 5～6cm, makes the 1st blade of distance cutting base portion from stromal surface 2cm ± 0.3cm; Cutting density is with overlapping being advisable not between the cuttage rear blade; The prescription of described root-growing agent A is the 0.8%IBA+2% thiophanate methyl.2% thiophanate methyl is that matching while using is in IBA solution among the root-growing agent A; Described percentage is mass fraction;
(4) cuttage: 5～6cm in the described matrix of cutting inserting step (1) that will process with root-growing agent, transplant a cutting the 1st blade of base portion from stromal surface 2cm ± 0.3cm to distance;
(5) manage after the cuttage:
A. water supply in media and ambient humidity management: after cuttage, start immediately spraying system and matrix spraying is watered permeable, and adopt the intermittent spraying mode to carry out follow-up water management, the mass water content of soil of matrix is 64～65%, from the beginning cuttage to the long root of cuttage seeding, keeps air humidity more than 80%; The automatic intermittent spraying mode is adopted in air humidity and water supply in media management, and fine day, half cloudy half fine, overcast and rainy 3 kinds of patterns are set; Before the taking root of the 1st～4 week, automatic spraying system 9:00 opens after the cuttage, and 18:00 closes.Fine spray intervals 10 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 15 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying.During the taking root of the 5th～10 week, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 12 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 20 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying.Enter the hardening phase after the 11st week beginning to take root, before the 11st thoughtful transplanting, automatic spraying system 10:00 opens, and 17:00 closes.Fine spray intervals 60 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 180 minutes, 30 seconds duration of spraying; Overcast and rainyly do not spray, keeping the mass water content of soil of matrix is 64～65%, avoids the blade face dry.
B. illumination and temperature treatment: adopt the natural daylight full exposure to process after the cuttage, the temperature on daytime is controlled at 18 ℃, and the temperature at night is controlled at 12 ℃, and the substrate temperature whole day remains on 18 ℃; The temperature of matrix is to be advisable a little more than nighttime temperature, in order to avoid Rooting effect survives after germinateing first;
C. the extermination of disease and insect pest: after the cuttage, close moisture spray in the afternoon after, adopting mass fraction is 1000 times of liquid sprayings of thiophanate methyl cuttage seeding of 50%, sprays once every 10 days, and in time removes the cutting of catching an illness;
D. nutrient management: before the long root of cutting, sprayed mass fraction every 5 days and be 0.1% urea, after cutting sends out roots, every 10 days, be that 0.1% urea liquid and mass fraction are that 0.1% potassium dihydrogen phosphate sprays once with mass fraction, close afternoon and carry out fertilizer after the moisture spray and spray; After 80～90 days, when cutting base portion formation diameter is the root ball of 1.5-3cm, cuttage seeding is transplanted the paramount 12cm that is, diameter is in the pot for growing seedlings of 14cm.
Statistics: alpine rose
Rhododendron' Nova Zembla' began to form callus in 25 days after cuttage, begin to have new root to grow after 60 days, rooting rate reaches 85%, well developed root system, and to form diameter be the root ball of 1.5～3cm transplanting a cutting base portion, without damage by disease and insect.
Embodiment 2
Except following difference was arranged, all the other steps were identical with embodiment 1, repeat no more:
(1) substrate composition: peat and perlitic volume ratio are 1 ︰ 4;
(2) cutting is selected: the alpine rose kind without damage by disease and insect, healthy growth of selecting seedling age life in 5 years
Rhododendron' Furnlvall daughter ' waters for maternal material as maternal material in the first day of clip cutting material, and the cutting top keeps 2 intact leafs, and transplanting a cutting has 3 nodes.
(3) processing of taking root before the cuttage: the base portion of will transplanting a cutting dips in natural air drying behind the root-growing agent A 20s.Used root-growing agent A prescription is the 1.0%IBA+4% thiophanate methyl.
(5) manage after the cuttage:
A. the mass water content of soil of matrix is 62～63%;
B. illumination and temperature treatment: the temperature on daytime is controlled at 25 ℃, and the temperature at night is controlled at 18 ℃, and the substrate temperature whole day remains on 22 ℃;
C. the extermination of disease and insect pest: adopting mass fraction is 1500 times of liquid sprayings of thiophanate methyl cuttage seeding of 50%, sprays once every 15 days, starts spraying system and sprays;
D. nutrient management: before the long root of cutting, sprayed mass fraction every 15 days and be 0.5% urea, wait transplant a cutting send out roots after, every 15 days, be that 0.5% urea and mass fraction are that 0.2% potassium dihydrogen phosphate sprays once with mass fraction, start spraying system and spray.
Statistics: alpine rose Rhododendron ' Furnlvall daughter ' began to form callus in 20 days after cuttage, begin to have new root to grow after 55 days, rooting rate reaches 86%, and well developed root system, forming diameter at the cutting base portion is the root ball of 1.5～3cm, without damage by disease and insect.
Embodiment 3
Except following difference was arranged, all the other steps were identical with embodiment 1, repeat no more:
(1) substrate composition: peat and perlitic volume ratio are 1 ︰ 4;
(2) cutting is selected:: the alpine rose kind without damage by disease and insect, healthy growth of selecting seedling age life in 4 years
Rhododendron' Furnlvall daughter ' waters for maternal material as maternal material in the first day of clip cutting material, and the cutting top keeps 2 intact leafs, and transplanting a cutting has 3 nodes.
(3) processing of taking root before the cuttage: the base portion of will transplanting a cutting dips in natural air drying behind the root-growing agent A 15s.Used root-growing agent A prescription is the 0.9%IBA+3% thiophanate methyl.
(5) manage after the cuttage:
A. the mass water content of soil of matrix is 60～61%; Entering the hardening phase after the 10th week began to take root is before extremely transplanting behind the long root of cuttage seeding, to open the shed ventilation air humidity is reduced to 70～79%, to promote Root growth.
B. illumination and temperature treatment: the temperature on daytime is controlled at 23 ℃, and the temperature at night is controlled at 15 ℃, and the substrate temperature whole day remains on 20 ℃;
C. the extermination of disease and insect pest: adopting mass fraction is 1300 times of liquid sprayings of thiophanate methyl cuttage seeding of 50%, sprays once every 12 days;
D. nutrient management: transplanting a cutting before the long root, spraying mass fraction every 10 days and be 0.3% urea, after cutting sends out roots, every 12 days, is that 0.3% urea and mass fraction are that 0.2% potassium dihydrogen phosphate sprays once with mass fraction.
Statistics: alpine rose Rhododendron ' Furnlvall daughter ' began to form callus in 22 days after cuttage, begin to have new root to grow after 57 days, rooting rate reaches 87%, and well developed root system, forming diameter at the cutting base portion is the root ball of 1.5～3cm, without damage by disease and insect.
Embodiment 4
Except used root-growing agent and using method difference, all the other steps are identical with embodiment 1, repeat no more.Used root-growing agent is root-growing agent B, and its prescription is: 0.015% IBA+1% thiophanate methyl; Thiophanate methyl among the described root-growing agent B be matching while using in 0.015% IBA solution, will transplant a cutting and be immersed among this root-growing agent B natural air drying behind the 24h take 15 as a bundle.
Statistics: alpine rose
Rhododendron' Nova Zembla' began to form callus in 25 days after cuttage, begin to have new root to grow after 60 days, rooting rate reaches 85%, well developed root system, and to form diameter be the root ball of 1.5～3cm transplanting a cutting base portion, without damage by disease and insect.
Embodiment 5
Except used root-growing agent and using method difference, all the other steps are identical with embodiment 2, repeat no more.Used root-growing agent is root-growing agent B, and its prescription is: 0.02% IBA+2% thiophanate methyl; Thiophanate methyl among the described root-growing agent B be matching while using in 0.02% IBA solution, will transplant a cutting and be immersed among this root-growing agent B natural air drying behind the 30h take 15 as a bundle.
Statistics: alpine rose kind
Rhododendron' Furnlvall daughter ' began to form callus in 25 days after cuttage, begin to have new root to grow after 54 days, and rooting rate reaches 89%, well developed root system, and to form diameter be the root ball of 1.5～3cm transplanting a cutting base portion, without damage by disease and insect.
Embodiment 6
Except used root-growing agent and using method difference, all the other steps are identical with embodiment 3, repeat no more.Used root-growing agent is root-growing agent B, and its prescription is: 0.018% IBA+1.5% thiophanate methyl; Thiophanate methyl among the described root-growing agent B be matching while using in 0.018% IBA solution, will transplant a cutting and be immersed among this root-growing agent B natural air drying behind the 36h take 15 as a bundle.
Statistics: alpine rose kind
Rhododendron' Furnlvall daughter ' began to form callus in 25 days after cuttage, begin to have new root to grow after 55 days, and rooting rate reaches 87%, well developed root system, and to form diameter be the root ball of 1.5～3cm transplanting a cutting base portion, without damage by disease and insect.
Embodiment 1-embodiment 6 shows:Of the present invention by selecting the aggregate measures such as suitable matrix, suitable root-growing agent and processing mode and suitable fertilising, temp. and humidity, effectively solved the difficult problem that the alpine rose cuttage is difficult for taking root, rooting rate can reach more than 85%, is one of method of the efficient breeding of alpine rose.
Embodiment 7
Root-growing agent of the cottage propagation of suitable alpine rose provided by the present invention and preparation method thereof
The prescription of root-growing agent A: 0.8～1.0%IBA+2～4% thiophanate methyl.
The prescription of root-growing agent B: 0.015～0.02% IBA+1～2% thiophanate methyl.
The preparation method of described root-growing agent A and root-growing agent B is as follows: be the highly concentrated solution that 95% alcohol is dissolved into IBA 10000～15000ppm with a small amount of mass fraction first, add again pure water and be diluted to this described concentration of filling a prescription, when processing cutting, again thiophanate methyl is dissolved in this IBA solution by the described concentration of prescription.Percentage described in root-growing agent A and the root-growing agent B is mass fraction.
Claims (9)
1. the cuttage breeding method of alpine rose may further comprise the steps:
(1) prepare in the seedbed: build shed in the seedbed, plastic covering film on shed, laying depth is the cutting medium of 12～15cm on the seedbed, below cutting medium, lay the ground hot line, cutting medium is peat and perlitic mixture, peat and perlitic volume ratio are 1:3～1:4, water after the matrix on the seedbed is smooth permeablely, use the liquor natrii hypochloritis of 1000ppm to ground sterilization in seedbed and the shed again;
(2) transplant a cutting to select: gathers life in 3～5 years without the alpine rose plant of the damage by disease and insect maternal material as cutting, 9～October, the semi-lignified branch that clip is given birth to then from this female parent material is for transplanting a cutting material, be that the stem section of 7～8cm is for transplanting a cutting with transplanting a cutting that material cuts, node more than 2 is arranged in the described cutting, and the top keeps 2～3 intact leafs;
(3) processing of taking root before the cuttage: the base portion of will transplanting a cutting dips in natural air drying behind root-growing agent A 10s～20s, and the base portion of maybe will transplanting a cutting is immersed among the root-growing agent B natural air drying behind 24h～36h; The prescription of described root-growing agent A is 0.8～1.0%IBA+2～4% thiophanate methyl, and the prescription of described root-growing agent B is 0.015～0.02% IBA+1～2% thiophanate methyl; Thiophanate methyl among root-growing agent A and the root-growing agent B is matching while using; Described percentage is mass fraction;
(4) cuttage: 5～6cm in the described matrix of cutting inserting step (1) that will process with root-growing agent makes and transplants a cutting the 1st blade of base portion from stromal surface 2cm ± 0.3cm;
(5) manage after the cuttage:
A. water supply in media and ambient humidity management: after cuttage, immediately matrix spraying is watered permeable, and adopt the intermittent spraying mode to carry out follow-up water management, the mass water content of soil of matrix is 60～65%, from the beginning cuttage to the long root of cuttage seeding, keeps air humidity more than 80%;
B. illumination and temperature treatment: adopt the natural daylight full exposure to process after the cuttage, the temperature on daytime is controlled at 18～25 ℃, and the temperature at night is controlled at 12～18 ℃, and the substrate temperature whole day remains on 18～22 ℃;
C. the extermination of disease and insect pest: after the cuttage, adopting mass fraction is 1000～1500 times of liquid sprayings of 50% thiophanate methyl cuttage seeding, sprays once every 10～15 days, and in time removes the cutting of catching an illness;
D. nutrient management: before the long root of cutting, sprayed one time 0.1～0.5% urea every 5～15 days, after cutting sends out roots, spray once every 10～15 days potassium dihydrogen phosphates with 0.1～0.5% urea and 0.1～0.2%; When cuttage seeding formation diameter is the root ball of 1.5～3cm, transplant to the seedling alms bowl; Described percentage is mass fraction.
2. the cuttage breeding method of alpine rose according to claim 1 is characterized in that: the described semi-lignified branch that clip is given birth to then from this female parent material of step (2) for transplant a cutting material be from this female parent material clip give birth to then diameter at the semi-lignified branch of 0.5～0.8cm for transplanting a cutting material.
3. the cuttage breeding method of alpine rose according to claim 1 and 2, it is characterized in that: the lower end in the described cutting of step (2) is cut into flat mouth.
4. the cuttage breeding method of alpine rose according to claim 1 and 2 comprises that also automatic spraying system is installed to carry out after step (5) cuttage management A. water supply in media and ambient humidity and manage described spraying and water permeable and the intermittent spraying mode is carried out follow-up water management.
5. the cuttage breeding method of alpine rose according to claim 3 comprises that also automatic spraying system is installed to carry out after step (5) cuttage management A. water supply in media and ambient humidity and manage described spraying and water permeable and the intermittent spraying mode is carried out follow-up water management.
6. the cuttage breeding method of alpine rose according to claim 4 is characterized in that: manage in the C. extermination of disease and insect pest and the D. nutrient management process spraying thiophanate methyl, urea and potassium dihydrogen phosphate with automatic spraying system after step (5) cuttage.
7. the cuttage breeding method of alpine rose according to claim 5 is characterized in that: manage in the C. extermination of disease and insect pest and the D. nutrient management process spraying thiophanate methyl, urea and potassium dihydrogen phosphate with automatic spraying system after step (5) cuttage.
8. the cuttage breeding method of alpine rose according to claim 4, it is characterized in that: it is that fine day, half cloudy half fine, overcast and rainy 3 kinds of patterns are set that the intermittent spraying mode of described automatic spraying system is carried out follow-up water management, and air humidity is more than 80% in the maintenance shed; The taking root early stage of the 1st～4 week after cuttage, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 10 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 15 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; During the taking root of the 5th～10 week, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 12 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 20 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; Before the 11st thoughtful transplanting, spraying system 10:00 opens, and 17:00 closes, fine spray intervals 60 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 180 minutes, 30 seconds duration of spraying; Overcast and rainyly do not spray, keeping the mass water content of soil of matrix is 60～65%.
9. the cuttage breeding method of alpine rose according to claim 5, it is characterized in that: it is that fine day, half cloudy half fine, overcast and rainy 3 kinds of patterns are set that the intermittent spraying mode of described automatic spraying system is carried out follow-up water management, and air humidity is more than 80% in the maintenance shed; The taking root early stage of the 1st～4 week after cuttage, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 10 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 15 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; During the taking root of the 5th～10 week, automatic spraying system 9:00 opens, and 18:00 closes, fine spray intervals 12 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 20 minutes, 30 seconds duration of spraying; Overcast and rainy spray intervals 60 minutes, 30 seconds duration of spraying; Before the 11st thoughtful transplanting, spraying system 10:00 opens, and 17:00 closes, fine spray intervals 60 minutes, 30 seconds duration of spraying; Half cloudy half fine spray intervals 180 minutes, 30 seconds duration of spraying; Overcast and rainyly do not spray, keeping the mass water content of soil of matrix is 60～65%.
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| CN1568670A (en) * | 2004-04-26 | 2005-01-26 | 上海市闵行区农业科学研究所 | Tissue culture and tachytelic propagating method for alpine rhododendron |
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