Background technology
" blue maiden " Chinese ilex (Ilex * meserveae S.Y.Hu. ' Blue Girl ') belongs to Aquifoliaceae (Aquifoliaceae) Ilex (Ilex) plant.The gardening crossbreed hybridizes generation by I.rugosa ' Long Island ' * I.aquifolium ' Lawrence White ', and the breeding people is K.K.Meserve., and this kind individual plant all is a female plant, and another kind " blue young boy " Chinese ilex all is a staminiferous plant.Evergreen shrubs, plant height 1-3 rice, the weak point that the branch tool is sparse and thin fine hair, blade olive-green, avette, crooked slightly, long 2.5-3.5 centimetre, wide is 1.3-1.8 centimetre, the base portion circle, most advanced and sophisticated sharp point, 4-6 piece little evagination spine of leaf margin tool, long 5 millimeters of petiole, the stem lavender, the inflorescence armpit is given birth to, floral white, fruit ruby look, sphere, footpath 6-8 millimeter, 4 pieces of pyrenes, pedicel pale red, long 6 millimeters, the florescence 4-6 month, the fruit phase 10-12 month.Like warmly, moistening, the environment that light is good requires fertile soil, and draining is good, is advisable to be rich in organic sand loam, and the growth fertilising of fully watering in season is in order to its well-grown.These seeds are suitable for the flower garden greening, can make hedgerow, landscape tree.In addition, this kind is a undershrub, and anti-pruning can be used as potted landscape and uses.
At present this kind seedling quantity is considerably less at home, and the seed germination that correlative study shows " blue maiden " Chinese ilex is difficulty relatively, and therefore " blue maiden " Chinese ilex resource lacks very much, and uses and also be " have city out of stock ".
Cottage propagation is the first-selected approach of present domestic solution nursery stock production.Existing report carries out the research of cottage propagation to Holly, but does not see have report research and utilization horse liver soil to carry out the cuttage of Holly.Utilize 3: 1 perlite and peat soil matrix that Central China Chinese ilex is carried out cuttage (seeing northern gardening o. 11th in 2010) as Huanghai Sea swallow, Qian Yongqiu utilizes 1: 3 perlite and peat soil matrix that purplefruit holly leaf is carried out cuttage (seeing Anhui agricultural circular, 2008 the 21st phases).The used matrix of cuttage requires relatively harsher, mostly is peat soil, perlite and so on, and these matrix exist watering property too big, loosely is difficult for shortcomings such as solid root, extensive cuttage cost be relatively large.The method of suiting measures to local conditions, saving into local large tracts of land industrialization cuttage " blue maiden " Chinese ilex yet there are no report.Horse liver soil is the distinctive a kind of soil in THE LOWER YANGTZE VALLEY area, and Sha Xingqiang does and can consolidate root, but wet water conservation, but water permeable ability is strong, is most appropriate to the moisture control that plugged ear is given birth to the front.
In sum, utilizing the cuttage of horse liver soil to breed " blue maiden " Chinese ilex fast is one of important channel that solves this seeds shortage of resources, and exploitation and the utilization and extention of " blue maiden " Chinese ilex all had very important meaning.
Summary of the invention
The technical problem that the present invention mainly solves is openly to utilize the method for quickly breeding of horse liver soil simple cuttage " blue maiden " Chinese ilex.
Adopt following technical scheme for solving this technology:
A) matrix treatments: after the pulverizing of horse liver soil, sneak into loess., pour in the cuttage pond that has puzzled horse liver soil and loess mixing according to 4: 1 ratios, cuttage pond specification is width 2m, height 0.3m, and length is decided according to cuttage quantity.Then, carbendazim is admixed in the soil, usage ratio is 4g/m
2
B) plugged ear is handled: early July, choose healthy and strong no damage by disease and insect branch as plugged ear.The truncation of plugged ear lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 10-15cm; Plugged ear lower cut place has the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Plugged ear keeps 1 pair of blade, and cuts off a half vane, and complete full lateral bud need be arranged.Plugged ear is with adopting with cuttage simultaneously.
C) HORMONE TREATMENT: use the IBA of 1000mg/kg to soak plugged ear hypomere 2min, insert slotting machine immediately, use sprinkling irrigation that matrix is irrigated.
D) full exposure spraying management: use the timer control of pump, water spraying time is controlled to be: fine day, particularly hot weather, spray time are 3min/ time, and to reach cooling effect, the cloudy day is lacked spray or do not spray, and is generally 12min/ time, and the rainy day does not spray.Temperature is 20-28 ℃, relative moisture 80%.
E) fertilizing management: step C) finish after 45d, use nutrient solution to spray the plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4H
2PO
4Form adds, and potash fertilizer is with KNO
3Form adds, and nitrogenous fertilizer is divided by NH
4H
2PO
4And KNO
3Form add outside, insufficient section is with (NH
4)
2SO
4Replenish.It is 25L that nutrient solution sprays volume.Cuttage 60d and 75d, repeat spraying 1 time.
The beneficial effect of the relative prior art of the present invention is: utilize horse liver soil simple cuttage " blue maiden " Chinese ilex, can obtain a large amount of " blue maiden " Chinese ilex seedlings fast and effectively, solve the problem of " blue maiden " Chinese ilex shortage of resources; By to matrix, plugged ear, hormone, sprinkling irrigation condition and survive back the choosing of nutrient formulation, can make " blue maiden " Chinese ilex cuttage survival rate reach more than 90%, reach seedling specification (more than the plant height 20cm) after surviving fast, can obtain " blue maiden " Chinese ilex high quality seedling efficiently.
Embodiment
Embodiment one
1, prepare in matrix, cuttage pond.After horse liver soil solarization dry grinding, sneak into loess., pour in the cuttage pond that has puzzled horse liver soil and loess mixing according to 4: 1 ratios (i.e. 4 go-cart horse livers soil and 1 go-cart loess), cuttage pond specification is the high 2m*5m*0.3m of length and width, and pond mesostroma height is 15cm.Then, the 40g carbendazim is admixed in the soil.Spray 2h, carbendazim is dissolved in the matrix.
2, plugged ear is handled.On July 1st, 2009, choose healthy and strong lifes in 6 years " blue maiden " the Chinese ilex seedling of 50 strains, the current-year branch of the healthy and strong no damage by disease and insect of clip is as plugged ear, and the clip plugged ear is 1000 altogether.The truncation of plugged ear lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 10cm; Plugged ear lower cut place should have the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Plugged ear keeps 1 pair of blade, and cuts off a half vane, and complete full lateral bud is arranged.After plugged ear is ready to, use the IBA of 1000mg/kg to soak plugged ear hypomere 2min, insert slotting machine immediately, insertion density is 10cm*10cm.Use and spray 1h, matrix is irrigated normal root.
3, full exposure spraying management: use the timer control of pump, water spraying time is controlled to be: fine day, and high temperature (more than 28 ℃) weather, spray time is 3min/ time, sprays 10h every day; At the cloudy day, spray time is 12min/ time, sprays 8h every day; Rainy day does not spray.The plugged ear surface temperature control is being 20-28 ℃, relative moisture 80%.
4,15d after the cuttage, the plugged ear base portion begins the callus of growing.25d, the callus differentiation begins to take root, 35d, rooting rate reaches 94.59%, the long 6.14cm that on average reaches of root.
5, fertilizing management: 45d after the cuttage, use nutrient solution to spray the plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4H
2PO
4Form adds, and potash fertilizer is with KNO
3Form adds, and nitrogenous fertilizer is divided by NH
4H
2PO
4And KNO
3Form add outside, insufficient section is with (NH
4)
2SO
4Replenish.Use sprayer to carry out foliage fertilizer and spray, volume is 25L.Cuttage 60d and 75d, repeat spraying 1 time.
6,70d after the cuttage, cuttage seeding is extracted the tender tip out, 90d after the cuttage, tender tip exhibition leaf finishes, and plant height on average reaches 20.21cm, the long average out to 13.34cm of root, the above radical of the long 10cm of root on average reaches 11.
Embodiment two
1, prepare in matrix, cuttage pond.After horse liver soil solarization dry grinding, sneak into loess., pour in the cuttage pond that has puzzled horse liver soil and loess mixing according to 4: 1 ratios (i.e. 4 go-cart horse livers soil and 1 go-cart loess), cuttage pond specification is the high 2m*10m*0.3m of length and width, and pond mesostroma height is 15cm.Then, the 80g carbendazim is admixed in the soil.Spray 2h, carbendazim is dissolved in the matrix.
2, plugged ear is handled.On July 10th, 2010, choose healthy and strong lifes in 6 years " blue maiden " the Chinese ilex seedling of 100 strains, the current-year branch of the healthy and strong no damage by disease and insect of clip is as plugged ear, and the clip plugged ear is 2000 altogether.The truncation of plugged ear lower cut, upper cut are cut sth. askew, otch level and smooth, crack-free; Length is 15cm; Plugged ear lower cut place should have the adventitious root primordia of hiding (being internode side shoot base portion, indefinite bud base portion or petiole base); Plugged ear keeps 1 pair of blade, and cuts off a half vane, and complete full lateral bud is arranged.After plugged ear is ready to, use the IBA of 1000mg/kg to soak plugged ear hypomere 2min, insert slotting machine immediately, insertion density is 10cm*10cm.Use and spray 1h, matrix is irrigated normal root.
3, full exposure spraying management: use the timer control of pump, water spraying time is controlled to be: fine day, and high temperature (more than 28 ℃) weather, spray time is 3min/ time, sprays 10h every day; At the cloudy day, spray time is 12min/ time, sprays 8h every day; Rainy day does not spray.The plugged ear surface temperature control is being 20-28 ℃, relative moisture 80%.
4,17d after the cuttage, the plugged ear base portion begins the callus of growing.23d, the callus differentiation begins to take root, 35d, rooting rate reaches 90.15%, the long 4.75cm that on average reaches of root.
5, fertilizing management: 45d after the cuttage, use nutrient solution to spray the plant blade face, nutrient composition is: N200mg/L, P33mg/L, K66mg/L, phosphate fertilizer is with NH
4H
2PO
4Form adds, and potash fertilizer is with KNO
3Form adds, and nitrogenous fertilizer is divided by NH
4H
2PO
4And KNO
3Form add outside, insufficient section is with (NH
4)
2SO
4Replenish.Use sprayer to carry out foliage fertilizer and spray, volume is 25L.Cuttage 60d and 75d, repeat spraying 1 time.
6,68d after the cuttage, cuttage seeding is extracted the tender tip out, 90d after the cuttage, tender tip exhibition leaf finishes, and plant height on average reaches 20.94cm, the long average out to 12.73cm of root, the above radical of the long 10cm of root on average reaches 9.