CN105830718A - Cutting propagation method for Torreya jackii - Google Patents
Cutting propagation method for Torreya jackii Download PDFInfo
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- CN105830718A CN105830718A CN201610228504.8A CN201610228504A CN105830718A CN 105830718 A CN105830718 A CN 105830718A CN 201610228504 A CN201610228504 A CN 201610228504A CN 105830718 A CN105830718 A CN 105830718A
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- cuttage
- cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Environmental Sciences (AREA)
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- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of asexual reproduction of plants and particularly provides a cutting propagation method for Torreya jackii. The method specifically comprises the following steps: (1) construction of a full-sunlight and automatic spraying cutting pool; (2) cutting shoot collection and cutting shoot preparation; (3) cutting; (4) maintenance management after cutting; (5) management after transplanting. According to the method, hormone treatment is adopted during cutting and transplanting, so that the cutting survival rate and the rooting rate are increased greatly, the plant growing conditions are improved, the propagation survival rate is high, the heredity changes little, management is convenient, large-scale propagation is facilitated, the endangered plant Torreya jackii can be saved effectively, and the existing background of the plant is enabled to move northwards.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, be specifically related to the cuttage breeding method of evergreen dungarunga Torreya jackii.
Background technology
Torreya jackii (Torreya jackii
Chan); have another name called Zhejiang Chinese torreya, Jia Shi Chinese torreya; belong to taxaceae Torreya; it it is the few survivors gymnosperm of remaining in the Tertiary Period in the Cenozoic; the distinctive rare tree of China; the wild plant that country's II level is laid special stress on protecting, the classification, the flora that belong to research all have more important meaning to the climatic similarity etc. of Quaternary ice age.Additionally, Torreya jackii material is hard, there is fragrance, can utensil processed.
Torreya jackii is evergreen dungarunga or is often multi-branched shrub；Branch is verticillate or to life, carries out or sprig is sagging；Leaf, to life, arranges into two row, and matter is hard, and wire lanceolar shape is long 3-13 centimetre, wide 3-4 millimeter, first has gradually thorn-like tip, base portion wedge shape, has short handle, above light green, micro-tool 2 and unconspicuous middle arteries and veins.Monoecism, male cone list leave armpit, tool 4-8 wheel, often 4 pieces of stamens of wheel.Oval or Long Circle, base portion have decussate bract, have short stalk.Female cone is born in leaf in pairs, the squamella that each tool 2 is raw to decussate bract and 1 piece of side, has short stalk；Ovule Dan Sheng, uprightly, only 1 globe daisy grows.Seed is all wrapped by meat aril, oval, long 2-3 centimetre, reddish yellow time ripe, by white powder, and embryo the deepest wrinkle significantly.
The Chinese torreya that comes into leaves is grown on mountain valley limes marginis moistening, nice and cool more, under hillside private parts hardwood forest, bamboo grove and in the rock gap of overhanging cliff.Being distributed mainly on China's Southern Zhejiang, western part, In Western Fujian Province, the north, some areas that east, Jiangxi Province, the north and Hubei Province are northern, distribution is narrower.Geographical coordinate: east longitude 112 °～122 °, north latitude 26 °～32 °, its distribution the northern fringe carries in Cao Fei ridge, Tonglu County Lu Ci township of Zhejiang Province and yellow township, Baokang County, Hubei Province, the big region of rivers and lakes one；Nan Yuan is in Mei Kou township, Taining County, Fujian Province；East is cheated along in An Ling township, Xianju County in Zhejiang Province stone length, west to Zixi County, Jiangxi Province and Jingan County state-owned forest farms.
Torreya jackii distribution is narrow, resource scarcity, and along with the mankind's activity destruction to natural environment, wild Torreya jackii quantity gradually reduces, and Torreya jackii seed maturity is long, and natural regeneration ability is poor.Temperature sand storage is used to pass the winter or directly sow, germination percentage the most about 0.5-1%.Thus it is badly in need of the artificial breeding taking measures to accelerate Torreya jackii.Institute of Botany from phase early 1960s i.e. start Torreya jackii introduce a fine variety work, through the Domestication tests of more than 50 years, Torreya jackii has adapted to the weather in Nanjing, preserve 2 strains and reach the big tree of 13 centimetres from the height of tree 6 meters in kind source, Zhejiang, the diameter of a cross-section of a tree trunk 1.3 meters above the ground, thus by the distributed areas of Torreya jackii from primary border northward extension.
In prior art, the cottage propagation for Torreya jackii is studied considerably less, owing to Torreya jackii is the rarest, suitableeer on market do not have, Torreya jackii seed maturity is the longest, natural regeneration ability is poor, temperature sand storage is used to pass the winter or directly sow, germination percentage is only 0.5-1%, in addition, Torreya jackii poor growth, requirement to environment is high, the 2 strain Torreya jackii that the Chinese Academy of Sciences of Jiangsu Province preserves, more than 50 year in the past, the height of tree 6 meters, 13 centimetres of chest neck, but it is the most solid, the situation of prior art does not utilize the large-scale breeding of Torreya jackii, therefore, the effective invisible propagation method of research Torreya jackii can save Endangered Plant Torreya jackii, and the existence background of these species is northwards elapsed.Cottage method disclosed in technology (application number 2013101994490) before applicant, rooting rate is relatively low, cottage propagation excessive cycle, and the application improves on this basis, it is thus achieved that excellent technique effect.
Summary of the invention
It is an object of the invention to provide the cuttage breeding method of a kind of Torreya jackii, the method can keep the good characteristic of Torreya jackii, and method is simple, and reproductive survival rate is high, for large-scale application preparing the offer technical guarantee of Torreya jackii nursery stock.
The cuttage breeding method of a kind of Torreya jackii that the present invention provides, specifically comprises the following steps that
1, the construction in full exposure auto spraying cuttage pond: select that illumination is sufficient, physical features is smooth, well-ventilated and draining convenient local construction cuttage pond.It is arranged over the automatic spraying system controlled by the time, does not spread sunshade net, for full exposure condition.Include several plastic crates in cuttage pond, in each basket, be covered with a gauze, to prevent matrix from spilling.Fragment of brick on both sides, the lower section pad in the direction that plastic crate is wide, to facilitate draining.
2, the preparation of cutting medium: cuttage pond uses horse liver soil: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, then press mixed-matrix gross weight 3% adds bio-organic fertilizer, and fully mixes, and laying depth is 20-22cm.
3, cuttings collection and fringe processed: cuttage Best Times is generally late June to late July.The collection of cuttings typically select overcast and rainy or morning dew the most dry time carry out, from Torreya jackii kind tree, sturdy branch is given birth in collection then, carries out fringe processed, and fringe bar makes processing preferably to be carried out in indoor, or in outdoor shady and cool place.Cuttings length 10-12cm, stays leaf 4-6 sheet, lower clip to be trimmed to 45 degree and cuts sth. askew, be conducive to taking root；
In under fringe bar, clip is immersed in cuttage hormone, 45-60min.
Described cuttage hormone is 30mg/L DA-6 amine fresh fat+50mg/L KT kinetin+40mg/L GGR6 root-inducing powder+25mg/LCPPU.
4, cuttage: the cuttings cuttage degree of depth is 3-4cm, density seeding row spacing is 4 cm × 4 cm；Water after having inserted, make cuttings be in close contact with matrix.
5, maintenance management after cuttage: cuttings curing condition is full exposure, and according to weather condition regulation water spray frequency and water spraying time length.
6, manage after transplanting: transplanting medium is horse liver soil: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, and add " father Bio likes me " efficient biologic-organic fertilizer by 5wt% in matrix, and after transplanting, fill Shi Dinggen hormone, described determine consisting of of root hormone: 30mg/L DA-6+50mg/L
KT+20mg/L GGR6+20mg/L CPPU+15mg/L GA3Contribute to taking root and survive.Seedling early stage is positioned under sunshade net, Jiao in case sunshine burns, and after waiting about 30 days slow seedlings, then moves to outdoor, and regards climate condition, spray weekly the most permeable.Additionally, change big nutritive cube, beneficially Torreya jackii root growth and stretching, extension in time.
In the present invention, in step 5, the automatic spraying system controlled with the time is sprayed water, its after cuttage first 30 days, and the time controls water spray requirement and is: within every 10 minutes in the case of fine day, spray 10 seconds；Within under overcast sky every 20-25 minute, spray 10 seconds；After 30 days, the time controls water spray requirement and is: within every 20 minutes in the case of fine day, spray 10 seconds；Within under overcast sky every 40-50 minute, spray 10 seconds.
The beneficial effects of the present invention is: its method is simple, reproductive survival rate is high, heredity change little, convenient management, beneficially large-scale breeding, can effectively save Endangered Plant Torreya jackii, and the existence background of these species is northwards elapsed.
For plant vegetative propagation field, how to utilize plant tissue differentiation regeneration plant, improve the reproductive number of plant, it is that to study important technology emphasis be also technological difficulties to scientist, hormone kind and the concentration effect to the highly significant that grows after cuttage, the application is found by great many of experiments, uses 30mg/L DA-6+50mg/L
KT+40mg/L GGR6+25mg/LCPPU, soak cuttage after cutting, cuttage efficiency can be greatly improved, the Torreya jackii speed of growth and rooting rate significantly improve, than the technology (cn103238448) before applicant, its survival rate of situation of taking root is greatly improved, for transplanting, applicant finds after-applied to determine root hormone transplanting through test in recent years, described determines consisting of of root hormone: 30mg/L DA-6+50mg/L
KT+20mg/L GGR6+20mg/LCPPU+15mg/LGA3, contributing to taking root survives, and after transplanting, survival rate is to 98%.
Horse liver soil is the distinctive a kind of soil of Lower Changjiang River region, and Sha Xingqiang does and can consolidate root, wet can water conservation, water permeable ability is strong, is most appropriate to the moisture before cuttage rooting and controls, its shortcoming is that water permeable ability is strong, and dehydration is too fast, easily air-dry, and single use can make cuttage seeding survive rear base portion dehydration to dry up.Red soil has good permeability and bigger salt balance ability, and turfy soil quality is soft the most fragmentary, has inspiratory, in subacidity, does not possess water-retaining property.Present inventor is through a large amount of creative works, carry out screening contrast experiment, from a large amount of cutting mediums, screen to go into action liver soil, red soil and these 3 kinds of matrix of turfy soil according to horse liver soil: red soil: turfy soil=3:3:2 weight ratio is used in combination, optimum Torreya jackii is taken root survival, interaction mutual induction between each matrix, reach to breathe freely, moisturizing, the effect of solid root.Cuttage rooting can either be rapidly promoted, the growth of plant after cutting survival can be promoted again.Composite substrate is utilized to cultivate Torreya jackii, it is possible to obtain Torreya jackii fast and effectively, the problem solving its shortage.
Detailed description of the invention
Embodiment 1
The construction of cuttage facility: cuttage place is positioned at Institute of Botany nursery, selects that illumination is sufficient, physical features is smooth, well-ventilated and draining convenient local construction cuttage facility.Cutting bed is the plastics Turnround basket of 55 cm × 18, cm × 36 cm, is covered with a gauze bottom basket, to prevent matrix from spilling.Fragment of brick is all padded on the both sides, lower section in the direction that Turnround basket is wide, to facilitate draining.The height of top 80 cm sets up the automatic spraying system controlled by the time；Top does not spread sunshade net, for full exposure condition.
Cutting medium: the preparation of cutting medium: cuttage pond uses horse liver soil: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, then press mixed-matrix gross weight 3% adds bio-organic fertilizer, and fully mixes, and laying depth is 20-22cm.Irrigate 2 times with water before cuttage, make matrix suction moisture.
Cuttings gathers and fringe processed: morning on July 15th, 2014, and from Torreya jackii kind tree, sturdy branch is given birth in collection then, and inserts in bucket by perpendicular for branch, has the water of deep 10 cm in bucket.Take indoor after gathering 300 branches to and carry out fringe processed, cutting length 10-12 cm, stay leaf 4-6 sheet, lower clip is trimmed to 45 degree and cuts sth. askew, and 1 branch can be cut into 1-2 cuttings, finally there are 488 cuttings for cuttage, in under fringe bar, clip is immersed in cuttage hormone, 45-60min.
Described cuttage hormone is 30mg/L DA-6+50mg/L
KT+40mg/L GGR6+25mg/LCPPU
Cuttage: the cuttage degree of depth is 3-4cm, density seeding row spacing is 4cm × 4cm.Water with water sprager after having inserted, make cuttings be in close contact with matrix.
Maintenance management after cuttage: cuttings curing condition is full exposure, first 30 days after cuttage, time control water spray requirement is: within every 10 minutes in the case of fine day, spray 10 seconds；Within under overcast sky every 20-25 minute, spray 10 seconds.After 30 days, the time controls water spray requirement and is: within every 20 minutes in the case of fine day, spray 10 seconds；Within under overcast sky every 40-50 minute, spray 10 seconds.Cuttage seeding moves into greenhouse in December.
Carrying out take root investigation and transplanting in January, 2015, its rooting rate is 94.3%, and condition survey of taking root the 460 strain cuttage seeding taken root, every strain average root of hair number is 4.51, average root length 6.02 cm, the thick 0.207cm of root.Not only save the time than the technology (rooting rate 75%, mean elements 2.73, root length 4.35, root thick 0.168) before the present inventor, reduce by the cultivation of nearly 3 months, and obtain significant effect on rooting rate and the parameter such as root length, radical.
Transplanting medium is horse liver soil: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, and add " father Bio likes me " efficient biologic-organic fertilizer by 5wt% in matrix, and after transplanting, fill Shi Dinggen hormone, described determine consisting of of root hormone: 30mg/L DA-6+50mg/L
KT+20mg/L GGR6+ 20mg/LCPPU+15mg/LGA3 (gibberellin) contributes to taking root and survives.Seedling early stage is positioned under sunshade net, Jiao in case sunshine burns, and after waiting about 30 days slow seedlings, then moves to outdoor, and regards climate condition, spray weekly the most permeable.Additionally, change big nutritive cube, beneficially Torreya jackii root growth and stretching, extension in time.Investigating on March 31st, 2015, acquisition survives transplanting Torreya jackii 450 strain altogether, and transplanting survival rate is: 97.8%.
Through the Torreya jackii plant that the inventive method obtains, growing way is strong, disease-resistant, it is possible to safety is passed the winter, and efficiently solves the vacancy of Torreya jackii market resource, has broad application prospects.
Claims (4)
1. the cuttage breeding method of a Torreya jackii, it is characterised in that comprise the steps:
(1) select that illumination is sufficient, physical features is smooth, well-ventilated and draining convenient local construction cuttage pond；It is arranged over the automatic spraying system controlled by the time, does not spread sunshade net, for full exposure condition；
(2) preparation of cutting medium: cuttage pond uses horse liver native: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, and according to 3% addition bio-organic fertilizer of gross weight in mixed-matrix, fully mix, laying depth is 20-22cm；
(3) cuttings collection and fringe processed: cutting time is that late June is to late July, the collection of cuttings select overcast and rainy or morning dew the most dry time carry out, from Torreya jackii kind tree, sturdy branch is given birth in collection then, carry out fringe processed, cuttings length 10-12cm, stay leaf 4-6 sheet, lower clip to be trimmed to 45 degree to cut sth. askew, be conducive to taking root；Again by during under fringe bar, clip is immersed in cuttage hormone, 45-60min；
(4) cuttage: the cuttings cuttage degree of depth is 3-4cm, waters after having inserted, makes cuttings be in close contact with the matrix of step (2)；
(5) maintenance management after cuttage: cuttings curing condition is full exposure, and according to weather condition regulation water spray frequency and water spraying time length；
(6) managing after transplanting: transplanting medium is horse liver soil: red soil: the mixed-matrix of turfy soil=3:3:2 weight ratio, and add " father Bio likes me " efficient biologic-organic fertilizer by 5wt% in matrix, and fill Shi Dinggen hormone after transplanting, contributing to taking root survives.
Method the most according to claim 1, it is characterised in that described cuttage hormone is 30mg/L
DA-6+50mg/L KT+40mg/L GGR6+25mg/L CPPU。
Method the most according to claim 1, it is characterised in that described to determine root hormone be 30mg/L
DA-6+50mg/L KT+20mg/L GGR6+20mg/L CPPU+15mg/L GA3。
4. method described in claim 1-3 is for the application of cottage propagation.
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| CN201610228504.8A CN105830718B (en) | 2016-04-13 | 2016-04-13 | A kind of cuttage breeding method of Torreya jackii |
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| CN201610228504.8A CN105830718B (en) | 2016-04-13 | 2016-04-13 | A kind of cuttage breeding method of Torreya jackii |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108966877A (en) * | 2018-07-10 | 2018-12-11 | 思南县华丰果蔬专业合作社 | A kind of method for culturing seedlings of cherry tree |
| CN110558130A (en) * | 2019-09-23 | 2019-12-13 | 台州学院 | Cutting method of cauliflower |
| CN112930891A (en) * | 2021-02-04 | 2021-06-11 | 浙江省林业科学研究院 | Method for full-exposure cutting seedling raising of Chinese torreya |
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| KR20130134480A (en) * | 2012-05-31 | 2013-12-10 | (주)아모레퍼시픽 | Extract of torreya sp.treated enzyme |
| CN103749302A (en) * | 2014-01-15 | 2014-04-30 | 江苏沿海地区农业科学研究所 | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings |
| CN105123687A (en) * | 2015-08-25 | 2015-12-09 | 何其家 | Compounding method of medium-concentration KT and glycerine gel |
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2016
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| CN102090256A (en) * | 2010-11-23 | 2011-06-15 | 江苏省中国科学院植物研究所 | Method for easily cutting IlexXmeserveae S.Y.Hu. 'Blue Girl' by using magan soil |
| KR20130134480A (en) * | 2012-05-31 | 2013-12-10 | (주)아모레퍼시픽 | Extract of torreya sp.treated enzyme |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108966877A (en) * | 2018-07-10 | 2018-12-11 | 思南县华丰果蔬专业合作社 | A kind of method for culturing seedlings of cherry tree |
| CN110558130A (en) * | 2019-09-23 | 2019-12-13 | 台州学院 | Cutting method of cauliflower |
| CN110558130B (en) * | 2019-09-23 | 2021-06-15 | 台州学院 | Cutting method of cauliflower |
| CN112930891A (en) * | 2021-02-04 | 2021-06-11 | 浙江省林业科学研究院 | Method for full-exposure cutting seedling raising of Chinese torreya |
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