CN105767012B - A kind of accelerator beneficial to plant cuttage root-taking - Google Patents
A kind of accelerator beneficial to plant cuttage root-taking Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The invention belongs to technical field of plant asexual propagation, specially a kind of accelerator beneficial to plant cuttage root-taking, the accelerator is by Sinorhizobium fredii zymotic fluid：Trichoderma aureoviride zymotic fluid：Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=4：3：4：3：2：5：2 are prepared, and the accelerator is the nutritional preparation specifically for the asexual cuttage root-taking of Torreya jackii, can form good microecosystem, be advantageous to plant growth, and form beneficial microbe colony, improve plant rhizosphere nutrient environment, cuttage root-taking and survival rate are greatly promoted, there is application prospect.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, and in particular to a kind of accelerator beneficial to plant cuttage root-taking.
Background technology
Torreya jackii（Torreya jackii Chan）, also known as Zhejiang Chinese torreya, Jia Shi Chinese torreyas, belong to taxaceae Torreya, be the Cenozoic
The few survivors gymnosperm of Tertiary Period remaining, the distinctive rare tree in China, the wild plant that national II levels are laid special stress on protecting, to research
The classification of category, flora have more important meaning to climatic similarity of Quaternary ice age etc..In addition, Torreya jackii material is hard
Firmly, there is fragrance, can utensil processed.
Torreya jackii is evergreen dungarunga or is often multi-branched shrub；Branch is verticillate or to life, carries out or sprig is sagging；Leaf pair
It is raw, two row are arranged into, matter is hard, wire lanceolar shape, long 3-13 centimetres, wide 3-4 millimeters, first has gradually thorn-like tip, base portion wedge shape, has
Short handle, above light green, micro- tool 2 and unconspicuous middle arteries.Monoecism, male cone list leave armpit, tool 4-8 wheels, often take turns 4 pieces
Stamen.Ellipse or Long Circle, base portion have decussate bract, have short stalk.Female cone is born in leaf in pairs, respectively has 2 pairs of interactions
To raw bract and the squamella of 1 piece of side life, has short stalk；Ovule Dan Sheng, uprightly, only 1 globe daisy development.Seed is all by meat
Matter aril is wrapped, oval, long 2-3 centimetres, reddish yellow when ripe, and by white powder, embryo is significantly inwardly deep to wrinkle.
The Chinese torreya that comes into leaves is grown on moistening, the mountain valley limes marginis felt nice and cool more, under hillside private parts hardwood forest, bamboo grove and overhanging cliff
Rock gap in.It is distributed mainly on China's Southern Zhejiang, western part, In Western Fujian Province, the north, Jiangxi Province east, the north and Hubei Province north
Some areas in portion, distribution are narrower.Geographical coordinate：112 °~122 ° of east longitude, 26 °~32 ° of north latitude, it is distributed the northern fringe
In Tonglu County Lu Ci townshiies of Zhejiang Province Cao Fei ridges and Hubei Province Baokang County yellow township, the band of the big region of rivers and lakes one；Nan Yuan is in Fujian Province Taining County
Mei Kou townshiies；Hole is grown in east along in Xianju County in Zhejiang Province An Ling townshiies stone, west to Jiangxi Province Zixi County and Jingan County state-owned forest farms.
Torreya jackii distribution is narrow, resource scarcity, with destruction of the mankind's activity to natural environment, wild Torreya jackii number
Amount gradually reduces, and Torreya jackii seed maturity is grown, and natural regeneration ability is poor.Passed the winter using the storage of warm sand or directly sowed,
Germination percentage only about 0.5-1%.Thus it is badly in need of the artificial breeding for taking measures to accelerate Torreya jackii.The Chinese Academy of Sciences of Jiangsu Province plant is ground
Study carefully since phase early 1960s is i.e. Torreya jackii introduce a fine variety work, by the Domestication tests of more than 50 years, Torreya jackii has been fitted
The weather in Nanjing is answered, has preserved 6 meters of 2 plants of height of trees from Zhejiang introduces a collection, the diameter of a cross-section of a tree trunk 1.3 meters above the ground up to 13 centimetres of big tree, so as to by Torreya jackii
Distributed areas from primary border northward extension.
Cutting propagation research in the prior art for Torreya jackii is considerably less, because Torreya jackii is extremely rare in the wild, city
More suitable on field not have, Torreya jackii seed maturity is very long, and natural regeneration ability is poor, is passed the winter using the storage of warm sand or directly broadcast
Kind, germination percentage is only 0.5-1%, in addition, Torreya jackii is slow-growing, the requirement to environment is high, and growth is extremely slow, every year
Growing height seems more precious rare less than 1 centimetre.2 plants of Torreya jackiis that the Chinese Academy of Sciences of Jiangsu Province preserves, more than 50 years
Past, but it is no so far solid, and the situation of prior art does not utilize the large-scale breeding of Torreya jackii, and therefore, research is come into leaves
Effective invisible propagation method of Chinese torreya can save Endangered Plant Torreya jackii, and the existence background of the species is northwards elapsed.It is existing
There is technology（Application number 2013101994490）Disclosed cottage method, rooting rate is relatively low, cutting propagation excessive cycle, the application
Inventor cooperates to be improved on the basis of existing technology with the Chinese Academy of Sciences, obtains excellent technique effect.
The content of the invention
Promote it is an object of the invention to provide a kind of accelerator beneficial to plant cuttage root-taking and using the accelerator
The method of Torreya jackii cuttage root-taking, this method can keep the good characteristic of Torreya jackii, and method is simple and easy, reproductive survival rate
Height, prepare Torreya jackii nursery stock for energy large-scale application and technical guarantee is provided.
Accelerator is used for the asexual reproduction method of Torreya jackii, comprises the following steps that：
1st, the construction in full exposure auto spraying cuttage pond：Select flat illumination abundance, physical features, well-ventilated and draining convenient
Local construction cuttage pond.Top is provided with the automatic spraying system by time control, does not spread sunshade net, is full exposure condition.Skewer
Inserting in pond includes several plastic crates, a gauze is covered with each basket, to prevent matrix from spilling.The lower section in the wide direction of plastic crate
The upper fragment of brick of both sides pad, to facilitate draining.
2nd, the preparation of cutting medium：Cuttage pond is using horse liver soil：Red soil：Turfy soil=4:3:3 weight than mixed base
Matter, bio-organic fertilizer then is added by the 3% of mixed-matrix gross weight, and fully mixed, laying depth 20-22cm.
3rd, cuttings collection and fringe processed：Cuttage Best Times are generally late June to late July.The collection of cuttings is typically selected
Rainy days or morning dew it is not dry when carry out, gathered from Torreya jackii kind tree and give birth to sturdy branch then, carry out fringe processed, fringe bar make plus
Work is preferably carried out indoors, or in outdoor shady and cool place.Cuttings length 10-12cm, stays leaf 4-6 pieces, and lower clip is trimmed to
45 degree of bevelings, are advantageous to take root；
Clip under fringe bar is immersed in accelerator, 30min.
The accelerator includes the raw material of following weight proportion：Sinorhizobium fredii zymotic fluid：Trichoderma aureoviride zymotic fluid：
Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=4：3：4：3：2：5：2；
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1 is (referring to Fei Shi
The research of putA genes, Tang Meiqiong etc. in Sinorhizobium HN01,2009)；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC32248
The candidiasis are specially candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC
NO.2276 (referring to CN101215537A)；
The preparation method of the accelerator is first by Sinorhizobium fredii, trichoderma aureoviride, candidiasis, lichens bud
Spore bacillus activates in a conventional manner, cultivates into bacterium solution viable count reaches 108Individual/gram acquisition zymotic fluid, by above-mentioned zymotic fluid
According to 4：3：4：3 mass ratios mix, and adding gibberellin, turf and dipotassium hydrogen phosphate according to weight proportion produces.
4th, cuttage：Cuttings cuttage depth is 3-4cm, and density seeding row spacing is the cm of 4 cm × 4；Watering after having inserted, make cuttings with
Matrix is in close contact.
5th, maintenance management after cuttage：Cuttings curing condition is full exposure, and adjusts water spray frequency and spray according to weather condition
Water time length.
6th, managed after transplanting：Transplanting medium is horse liver soil：Red soil：Turfy soil=4:3:3 weight than mixed-matrix, and
According to 8% weight than addition accelerator in mixed-matrix, helping to take root survives.Seedling is positioned under sunshade net early stage, with
Bright Jiao of anti-sunshine, after waiting 30 days or so slow seedlings, then moves to outdoor, and regards climate condition, sprays weekly once permeable.In addition,
Big nutritive cube is changed in time, is advantageous to Torreya jackii root growth and stretching, extension.
In the present invention, in step 5, sprayed water with the automatic spraying system of time control, its first 30 day after cuttage,
Time control water spray requirement be：Spray 10 seconds within every 10 minutes in the case of fine day；Sprayed 10 seconds per 20-25 minutes under overcast sky；30
After it, time control water spray requirement is：Spray 10 seconds within every 20 minutes in the case of fine day；Under overcast sky 10 are sprayed per 40-50 minutes
Second.
The beneficial effects of the present invention are：Its method is simple and easy, and reproductive survival rate is high, and heredity changes small, manager
Just, beneficial to large-scale breeding, Endangered Plant Torreya jackii can be effectively saved, and the existence background of the species is northwards elapsed.
For plant vegetative propagation field, how using plant tissue differentiation regeneration plant, the reproductive number of plant is improved,
It is that the important technology emphasis of scientist's research is also technological difficulties, accelerator of the invention is specifically for the asexual cuttage of Torreya jackii
The nutritional preparation taken root, it the moderate concentration of gibberellin, stimulates the stretching, extension of root cell prosperous by substantial amounts of investigation and experiment
Contain, collocation Sinorhizobium fredii, trichoderma aureoviride, candidiasis, bacillus licheniformis form a good microecosystem
System, brings into matrix after immersion and is built with beneficial to plant growth and prevents and treats the beneficial microbe colony of pest and disease damage, improves plant roots
Border nutrient environment, it, which is metabolized, can also strengthen the decomposition of soil organic matter, and reasonable compatibility between each strain, symbiosis is coordinated, mutually not short of money
Anti-, microorganism secretion root system regulator and ablastins can take good care of the rotten mould root rot of seedlings root infection, phytophthora root rot, bacterium
Property the root rot and sprout term disease such as base rot disease and damping-off, samping off, dipotassium hydrogen phosphate is continuing to supply nutrition, maintains cuttage seeding
The strong impetus, greatly improves the adaptability of plant, improves whole survival rate, and turf aeration is good, adsorption capacity is strong, and water conservation is protected
Fertilizer, be advantageous to microbial activities, strengthen biological property, rich in organic matter, humic acid and other nutritional ingredients.
Horse liver soil is a kind of distinctive soil of Lower Changjiang River region, Sha Xingqiang, dry to consolidate root, it is wet can water conservation, water permeable ability
By force, the control of the moisture before cuttage rooting is most appropriate to, its shortcoming is that water permeable ability is strong, and dehydration is too fast, easily air-dries, single use
Cuttage seeding can be made to survive rear base portion dehydration to dry up.Red soil has good permeability and larger salt balance ability, turf
Soil property it is soft easily it is fragmentary, have it is inspiratory, in subacidity, do not possess water-retaining property.Present inventor is by a large amount of creative
Work, carries out screening contrast experiment, and must go into action liver soil, red soil and turfy soil these four matrix are screened from a large amount of cutting mediums
According to horse liver soil：Red soil：Turfy soil=4:3:3 weight than being used in combination, take root survival by optimum Torreya jackii, mutual between each matrix
Make mutual induction, reach ventilative, moisturizing, the effect of solid root.Cuttage rooting can either be rapidly promoted, plant after cutting survival can be promoted again
Growth.Using composite substrate culture Torreya jackii, Torreya jackii can be fast and effectively obtained, solves the problems, such as that its is short.
Embodiment
Embodiment 1
The construction of cuttage facility：Select flat illumination abundance, physical features, well-ventilated and the convenient local construction cuttage of draining
Facility.Cutting bed is the cm of the cm of 55 cm × 36 × 18 plastics Turnround basket, and basket bottom is covered with a gauze, to prevent matrix from spilling.
Fragment of brick is padded on the lower section both sides in the wide direction of Turnround basket, to facilitate draining.The cm of top 80 height is set up by time control
Automatic spraying system；Top does not spread sunshade net, is full exposure condition.
Cutting medium：The preparation of cutting medium：Cuttage pond is using horse liver soil：Red soil：Turfy soil=4:3:3 weight than
Mixed-matrix, bio-organic fertilizer then is added by the 3% of mixed-matrix gross weight, and fully mixed, laying depth 20-
22cm.Irrigated 2 times with water before cuttage, matrix is suctioned moisture.
Cuttings gathers and fringe processed：Morning on July 17th, 2014, gathered from Torreya jackii kind tree and give birth to sturdy branch then, and
By the perpendicular water for inserting in bucket, there is 10 cm deeply in bucket of branch.Take interior after 300 branches of collection to and carry out fringe processed, cuttings length
10-12 cm are spent, stay leaf 4-6 pieces, lower clip is trimmed to 45 degree of bevelings, and 1 branch can be cut into 1-2 cuttings, finally there are 479
Individual cuttings is used for cuttage, clip under fringe bar is immersed in accelerator, 30min.
The accelerator includes the raw material of following weight proportion：Sinorhizobium fredii zymotic fluid：Trichoderma aureoviride zymotic fluid：
Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=4：3：4：3：2：5：2；
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1 is (referring to Fei Shi
The research of putA genes, Tang Meiqiong etc. in Sinorhizobium HN01,2009)；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC No.32248
The candidiasis are specially candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is specially bacillus licheniformis（Bacilluslicheniformis）CGMCC
NO.2276 (referring to CN101215537A)；
The preparation method of the accelerator is first by Sinorhizobium fredii, trichoderma aureoviride, candidiasis, lichens bud
Spore bacillus activates in a conventional manner, cultivates into bacterium solution viable count reaches 108Individual/gram acquisition zymotic fluid, by above-mentioned zymotic fluid
According to 4：3：4：3 mass ratios mix, and adding gibberellin, turf and dipotassium hydrogen phosphate according to weight proportion produces.
Cuttage：Cuttage depth is 3-4cm, and density seeding row spacing is 4cm × 4cm.Watered after having inserted with water sprager, make to insert
Fringe is in close contact with matrix.
Maintenance management after cuttage：Cuttings curing condition is full exposure, first 30 days after cuttage, and time control water spray requires
For：Spray 10 seconds within every 10 minutes in the case of fine day；Sprayed 10 seconds per 20-25 minutes under overcast sky.After 30 days, time control water spray
It is required that it is：Spray 10 seconds within every 20 minutes in the case of fine day；Sprayed 10 seconds per 40-50 minutes under overcast sky.Cuttage seeding is moved in December
Enter greenhouse.
Carry out take root investigation and transplanting in January, 2015, its rooting rate is 92.9%, and the 445 plants of cuttage seedings taken root are given birth to
Root condition survey, every plant of average root of hair number is 4.33, the average cm of root long 6.03, thick 0.227 cm of root.Than prior art（It is raw
Root rate 75%, mean elements 2.73, root long 4.35, root thick 0.168）Not only save the time, reduce by the culture of nearly 3 months, and
Significant effect is obtained in the parameter such as rooting rate and root long, radical.
Transplanting medium is horse liver soil：Red soil：Turfy soil=4:3:3 weight than mixed-matrix, and pressed in mixed-matrix
According to 8% weight ratio addition accelerator, helping to take root survives.Seedling is positioned under sunshade net early stage, is burnt to prevent sunshine burnt,
After 30 days or so slow seedlings, outdoor is then moved to, and regards climate condition, is sprayed weekly once permeable.In addition, big nutrition is changed in time
Alms bowl, be advantageous to Torreya jackii root growth and stretching, extension.Investigated on March 31st, 2015, obtain survive 435 plants of Torreya jackii of transplanting altogether,
Transplanting survival rate is：97.8%.
The Torreya jackii plant obtained through the inventive method, growing way is strong, disease-resistant, can safely pass the winter, efficiently solve length
The vacancy of leaf Chinese torreya market resource, has broad application prospects.
Claims (3)
1. a kind of accelerator beneficial to plant cuttage root-taking, it is characterised in that the accelerator includes the original of following weight proportion
Material：Sinorhizobium fredii zymotic fluid：Trichoderma aureoviride zymotic fluid：Candida fermented liquid：The lichen bacillus ferments liquid：It is red
Mycin：Turf：Dipotassium hydrogen phosphate=4：3：4：3：2：5：2；The plant is Torreya jackii.
2. accelerator according to claim 1, it is characterised in that
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC No.32248
The candidiasis are candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276.
3. accelerator according to claim 1 or 2, it is characterised in that the preparation method of the accelerator is：It will take first
Family name's Sinorhizobium, trichoderma aureoviride, candidiasis, bacillus licheniformis activate, cultivate into bacterium solution and live in a conventional manner
Bacterium number reaches 108Individual/gram, zymotic fluid is obtained, then by above-mentioned zymotic fluid according to 4：3：4：3 weight proportions mix, and finally add
Gibberellin, turf and dipotassium hydrogen phosphate produce.
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| CN106386902A (en) * | 2016-08-31 | 2017-02-15 | 山东胜伟园林科技有限公司 | Acetic starch-containing rooting agent and preparation method thereof |
| CN106900769A (en) * | 2017-03-02 | 2017-06-30 | 申翰昭 | The growth regulating composition and its application process of bulbus fritillariae cirrhosae |
| CN106748323A (en) * | 2017-03-02 | 2017-05-31 | 申翰昭 | The growth regulating composition and its application process of Chinese herbaceous peony |
| CN108605987B (en) * | 2018-04-09 | 2021-03-19 | 南宁汉和生物科技股份有限公司 | Composite solution for promoting rooting of Chinese flowering crabapple cuttings |
| CN109121778B (en) * | 2018-08-07 | 2022-01-28 | 云南元江大有为食品有限公司 | Extraction method of bradyrhizobium extract and application of bradyrhizobium extract in mango grafting |
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| 微生物源的植物生长促进剂研究新进展;袁志林等;《农药市场信息》;20050228;第15-17页 * |
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