CN103493677A - Method of cutting propagation of peony immature stem - Google Patents
Method of cutting propagation of peony immature stem Download PDFInfo
- Publication number
- CN103493677A CN103493677A CN201310505898.3A CN201310505898A CN103493677A CN 103493677 A CN103493677 A CN 103493677A CN 201310505898 A CN201310505898 A CN 201310505898A CN 103493677 A CN103493677 A CN 103493677A
- Authority
- CN
- China
- Prior art keywords
- bacterium liquid
- cuttings
- herbaceous peony
- cultivation
- chinese herbaceous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y02P60/216—
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the field of plant tissue cultivation, and particularly relates to a method of cutting propagation of a peony immature stem. The method includes the steps of material selection, cutting cultivation, indoor management, oversummer maintenance, overwinter management and strong seedling cultivation. By using low-frequency ultrasonic waves to conduct processing, rooting can be promoted, plant cell division can be remarkably accelerated and induced, the cell growth is stimulated, protein synthesis of protoplast can be accelerated, and the adventitious bud reproducibility can be improved. Due to the fact that intermittent irradiation is adopted, no drastic cavitation effect can be produced, and partial damage to cells caused by the ultrasonic waves can be avoided. Due to the fact that cultivation and rooting are conducted in the dark cultivation process, growth inhibitor is greatly reduced, and rooting is facilitated. The complete and specific method including the steps of oversummer maintenance, auxiliary bud inducing, overwinter management and strong seedling cultication is provided, and therefore application of the peony rapid cutting propagation technology in actual production is promoted, and the method has significance in large-scale production of peony seedlings.
Description
Technical field
The invention belongs to the Plant Tissue Breeding field, be specifically related to the method for the tender stem cottage propagation of a kind of Chinese herbaceous peony.
Background technology
Chinese herbaceous peony (Paeonia lactiflora Pall), be Paeoniaceae Paeonia perennial root herbaceous plant, and the reputation of " flower phase " is just arranged from ancient times, and its flower has U.S. of look, perfume (or spice), rhythm concurrently, and in blooming, its flower is very large, and flower holds handsome.Chinese herbaceous peony is as Chinese traditional famous flower, and except at afforestation, the aspect such as medicinal, Chinese herbaceous peony application prospect aspect fresh cut-flowers production is also wide.But the production of Chinese herbaceous peony seedling relatively lags behind, and can't meet the need of market.Most Herbaceous peony kinds due to female, stamen lobe rate is high loses or partly lose fecundity, and seed propagation can not keep maternal character, therefore adopt traditional offshoot to be bred more.The living Chinese herbaceous peony seedling of one strain 3 years adopts the offshoot breeding, only can get the strain of 3-5 stalk, and reproduction coefficient is lower.
It is simple, with low cost that cottage propagation has, and the advantage that reproduction coefficient is larger is applied on the Fast-propagation of many gardening plants.Chinese herbaceous peony is herbaceos perennial, and the acrial part stem breaks up without axillalry bud, even traditional view is thought the Chinese herbaceous peony cuttage root-taking, and also can not seedling owing to lacking growing point.Therefore the research of Chinese herbaceous peony cuttage and quick-propagation is started late, and mainly concentrates on cuttage rooting and the cuttings initial stage survives aspect.Zhang Yandong etc. (2009) select 10 Central Plains Cultivars of Chinese Herbaceous Peonies to carry out the cuttage test, think that its survival rate (take root and form callus) is 20%-93%, and whole survival rate is 86%.
At present, still have that the adventitious bud proliferation rate is low, group training seedling rooting culture survives the problems such as difficulty, the requirement that does not also reach actual application level.In addition, take root that more summer maintenance, the axillalry bud of seedling induced, the research of the aspects such as overwinter management and strong seedling cultivation also exists large stretch of blank.Therefore, if can solve the problems referred to above of Chinese herbaceous peony cuttage and quick-propagation, make Chinese herbaceous peony cuttage and quick-propagation technology reach application level, significant to the large-scale production of Chinese herbaceous peony seedling.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art; the method of the tender stem cottage propagation of a kind of Chinese herbaceous peony is provided; not only can greatly improve the adventitious bud proliferation rate; but also more summer maintenance, the axillalry bud of the seedling that proposed to take root are induced, the concrete grammar of overwinter management and strong seedling cultivation; promoted the application of Chinese herbaceous peony cuttage and quick-propagation technology in actual production, significant to the large-scale production of Chinese herbaceous peony seedling.
The technical solution used in the present invention is: the method for the tender stem cottage propagation of a kind of Chinese herbaceous peony comprises the following steps:
Step 1, draw materials
The first tenday period of a month in late April to May, the Chinese herbaceous peony SPRING WHEAT BEFORE AND AFTER FLOWERING was chosen growing way stalwartness, disease-free tender stem, the tender stem of Chinese herbaceous peony was cut to be made as have after three joints standbyly as cuttings, and the cuttings upper cut is apart from upper bud 1cm;
Step 2, cuttage are cultivated
Remove cuttings a slice compound leaf bottom, soak the 30-60 min of 1-2cm place, cuttings lower end with root-growing agent, then cuttings being placed in to temperature is 20-25 ℃, standing 12h in the greenhouse that relative moisture is 85-90%, insert cuttings in the triangular flask that matrix is housed and secretly cultivate afterwards, and insertion depth is 3cm, triangular flask is applied to the ultrasonic that frequency is 25kHz simultaneously, the every work 5s of ultrasonic, stop 5～8s, amounts to work 8min; Described matrix is comprised of peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, the volume ratio of each component is 3:1:3:1:0.005:0.005, and wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10
9individual/mL;
Step 3, Room management
Keeping the temperature in greenhouse is 20～30 ℃, relative moisture 75～85%, and cuttings is carried out to intermittent spraying, spray method is: before taking root, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration, the KH that the mass percent concentration of rear every 5 d sprays of taking root is 0.2%
2pO
4the urea liquid that solution and mass percent concentration are 0.2% is to supplement the nutrients;
Step 4, more summer maintenance
Cuttage rooting started hardening after 28～32 days, entered the more maintenance stage in summer after hardening completes, and in greenhouse, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration; Start to carry out axillalry bud after entering more the maintenance stage in summer and induce, during regularly spray composite fertilizer's solution and trace element, and smear kryptoblast bottom with the rudiment agent; Described rudiment agent is fast by 6-benzyl gland, kinetin, gibberellin, glycerine and lanolin form, and the each component proportioning is the fast 0.04%-0.10% of 6-benzyl gland, kinetin 0.01%-0.05%, gibberellin 0.005%-0.02%, methyl-sulfoxide 1.0-3.0%, glycerine 1% and lanolin 94.93%-97.59%;
Step 5, overwinter management
Enter after the winter seedling of taking root and be placed in warm canopy, keep temperature to be not less than 0 ℃, relative moisture is not less than 70%;
Step 6, strong seedling cultivation
The seedling of taking root after Second Year thaws March takes out in container, together with the matrix agglomerate, is transplanted into land for growing field crops, waters once permeablely, plays the high rib insulation of 8～12cm soil moisture conservation.
The preparation method of the described matrix of step 2 is: for 3:1:3:1:0.005:0.005 takes peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10 by volume
9individual/mL; Then peat, perlite, vermiculite and human and animal excreta are stirred in proportion, and carry out sterilizing, add azotobacter chroococcum bacterium liquid afterwards in matrix, while prepare using, within first 7 days, add hay bacillus bacterium liquid.
Bacillus subtilis in the present invention (Bacillus subtilis) culture presevation is numbered ATCC6633, is preserved in American Type Culture Collecti, claims again American type culture collection (ATCC), is positioned at the Virginia, The United States state.Bacillus subtilis ATCC6633 in the present invention just examines bio tech ltd purchased from Nanjing.
Azotobacter chroococcum in the present invention (Azotobacter chroococcum) culture presevation numbering ACCC10098, contain in China Committee for Culture Collection of Microorganisms's common micro-organisms center.Azotobacter chroococcum ATCC6633 in the present invention is purchased from Chinese agriculture microorganism fungus kind preservation administrative center.
Beneficial effect of the present invention has the following aspects:
One, the ultrasonic that proportion of the present invention is 25kHz is processed, and frequency is than the low energy hestening rooting, obviously accelerate and the division of inducing plant cell, and stimulating cellular growth, the protein that accelerates protoplast synthesizes, and has improved the adventitious bud proliferation rate.And in the present invention, the every work 5s of ultrasonic, stop 5～8s, amount to work 8min, there is the short-term gap between irradiation, therefore can not produce fierce cavitation effect, avoided the ultrasonic local damage that cell is caused.
Two, growth inhibitor is a kind of material that blanketing is arranged taking root in plant corpus, in life cycle along with the prolongation increased content of time.And the plant that the material that cottage propagation is got has grown up to from some, its body contains the raised growth inhibitor, has greatly suppressed to take root after cuttage.The present invention has greatly reduced growth inhibitor by taking to cultivate to take root in dark the cultivation, is beneficial to and takes root.
Three, contain azotobacter chroococcum in the matrix of root induction of the present invention and greatly improved intramatrical nitrogen content, promoted the growth of plant, be beneficial to the growth of seedling.In addition, the secondary metabolite of azotobacter chroococcum produces growth hormone, has further promoted the generation of inducing of root.
Four, because bacillus subtilis is widespread in nature, nontoxic to people and animals, free from environmental pollution, there is significant antibacterial activity and extremely strong anti-adversity ability, and bacillus subtilis can promote growing of plant seed, seedling and root system, strengthened the disease resistance of plant.Matrix of the present invention adds bacillus subtilis both can play disease-resistant effect before use also can arrive the effect that promotes seedling and root growth and development, has improved rooting rate.
Five, the invention provides that complete more summer maintenance, axillalry bud are induced, the concrete grammar of overwinter management and strong seedling cultivation, promoted the application of Chinese herbaceous peony cuttage and quick-propagation technology in actual production, significant to the large-scale production of Chinese herbaceous peony seedling.
Six, the present invention is by take multiple channel to promote plant establishment, growth simultaneously, and reproduction rate is high as a result, and whole survival rate reaches 94%, survives seedling growing way stalwartness, and proterties is good.
Embodiment
Below embodiments of the invention are elaborated, the present embodiment is implemented take technical solution of the present invention under prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The method of the tender stem cottage propagation of a kind of Chinese herbaceous peony comprises the following steps:
Step 1, draw materials
The first tenday period of a month in late April to May, the Chinese herbaceous peony SPRING WHEAT BEFORE AND AFTER FLOWERING was chosen growing way stalwartness, disease-free tender stem, the tender stem of Chinese herbaceous peony was cut to be made as have after three joints standbyly as cuttings, and the cuttings upper cut is apart from upper bud 1cm;
Step 2, cuttage are cultivated
Remove cuttings a slice compound leaf bottom, soak the 30-60 min of 1-2cm place, cuttings lower end with root-growing agent, then cuttings being placed in to temperature is 20-25 ℃, standing 12h in the greenhouse that relative moisture is 85-90%, insert cuttings in the triangular flask that matrix is housed and secretly cultivate afterwards, and insertion depth is 3cm, triangular flask is applied to the ultrasonic that frequency is 25kHz simultaneously, the every work 5s of ultrasonic, stop 5～8s, amounts to work 8min; Described matrix is comprised of peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, the volume ratio of each component is 3:1:3:1:0.005:0.005, and wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10
9individual/mL;
Step 3, Room management
Keeping the temperature in greenhouse is 20～30 ℃, relative moisture 75～85%, and cuttings is carried out to intermittent spraying, spray method is: before taking root, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration, the KH that the mass percent concentration of rear every 5 d sprays of taking root is 0.2%
2pO
4the urea liquid that solution and mass percent concentration are 0.2% is to supplement the nutrients;
Step 4, more summer maintenance
Cuttage rooting started hardening after 28～32 days, entered the more maintenance stage in summer after hardening completes, and in greenhouse, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration; Start to carry out axillalry bud after entering more the maintenance stage in summer and induce, during regularly spray composite fertilizer's solution and trace element, and smear kryptoblast bottom with the rudiment agent; Described rudiment agent is fast by 6-benzyl gland, kinetin, gibberellin, glycerine and lanolin form, and the each component proportioning is the fast 0.04%-0.10% of 6-benzyl gland, kinetin 0.01%-0.05%, gibberellin 0.005%-0.02%, methyl-sulfoxide 1.0-3.0%, glycerine 1% and lanolin 94.93%-97.59%;
Step 5, overwinter management
Enter after the winter seedling of taking root and be placed in warm canopy, keep temperature to be not less than 0 ℃, relative moisture is not less than 70%;
Step 6, strong seedling cultivation
The seedling of taking root after Second Year thaws March takes out in container, together with the matrix agglomerate, is transplanted into land for growing field crops, waters once permeablely, plays the high rib insulation of 8～12cm soil moisture conservation.
The preparation method of the described matrix of step 2 is: for 3:1:3:1:0.005:0.005 takes peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10 by volume
9individual/mL; Then peat, perlite, vermiculite and human and animal excreta are stirred in proportion, and carry out sterilizing, add azotobacter chroococcum bacterium liquid afterwards in matrix, while prepare using, within first 7 days, add hay bacillus bacterium liquid.
Embodiment 1
The method of the tender stem cottage propagation of a kind of Chinese herbaceous peony comprises the following steps:
Step 1, draw materials
April 25, the Chinese herbaceous peony SPRING WHEAT BEFORE AND AFTER FLOWERING is chosen growing way stalwartness, disease-free tender stem, the tender stem of Chinese herbaceous peony is cut to be made as have after three joints standbyly as cuttings, and the cuttings upper cut is apart from upper bud 1cm;
Step 2, cuttage are cultivated
Remove cuttings a slice compound leaf bottom, soak 45 min of 1-2cm place, cuttings lower end with root-growing agent, then cuttings being placed in to temperature is 22 ℃, standing 12h in the greenhouse that relative moisture is 87%, insert cuttings in the triangular flask that matrix is housed and secretly cultivate afterwards, and insertion depth is 3cm, triangular flask is applied to the ultrasonic that frequency is 25kHz simultaneously, the every work 5s of ultrasonic, stop 6s, amounts to work 8min; Described matrix is comprised of peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, the volume ratio of each component is 3:1:3:1:0.005:0.005, and wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10
9individual/mL;
Step 3, Room management
Keeping the temperature in greenhouse is 25 ℃, relative moisture 80%, and cuttings is carried out to intermittent spraying, spray method is: before taking root, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration, the KH that the mass percent concentration of rear every 5 d sprays of taking root is 0.2%
2pO
4the urea liquid that solution and mass percent concentration are 0.2% is to supplement the nutrients;
Step 4, more summer maintenance
Cuttage rooting started hardening after 28～32 days, entered the more maintenance stage in summer after hardening completes, and in greenhouse, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration; Start to carry out axillalry bud after entering more the maintenance stage in summer and induce, during regularly spray composite fertilizer's solution and trace element, and smear kryptoblast bottom with the rudiment agent; Described rudiment agent is fast by 6-benzyl gland, kinetin, gibberellin, glycerine and lanolin form, and the each component proportioning is that 6-benzyl gland is fast 0.07%, kinetin 0.03%, gibberellin 0.012%, methyl-sulfoxide 2.0%, glycerine 1% and lanolin 96.26%;
Step 5, overwinter management
Enter after the winter seedling of taking root and be placed in warm canopy, keep temperature to be not less than 0 ℃, relative moisture is not less than 70%;
Step 6, strong seedling cultivation
The seedling of taking root after Second Year thaws March takes out in container, together with the matrix agglomerate, is transplanted into land for growing field crops, waters once permeablely, plays the high rib insulation of 10cm soil moisture conservation.
The preparation method of the described matrix of step 2 is: for 3:1:3:1:0.005:0.005 takes peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10 by volume
9individual/mL; Then peat, perlite, vermiculite and human and animal excreta are stirred in proportion, and carry out sterilizing, add azotobacter chroococcum bacterium liquid afterwards in matrix, while prepare using, within first 7 days, add hay bacillus bacterium liquid.
Result shows: the present invention is by take multiple channel to promote plant establishment, growth simultaneously, and reproduction rate is high as a result, and whole survival rate reaches 94%, survives seedling growing way stalwartness, and proterties is good.
Claims (2)
1. the method for the tender stem cottage propagation of Chinese herbaceous peony, is characterized in that, comprises the following steps:
Step 1, draw materials
The first tenday period of a month in late April to May, the Chinese herbaceous peony SPRING WHEAT BEFORE AND AFTER FLOWERING was chosen growing way stalwartness, disease-free tender stem, the tender stem of Chinese herbaceous peony was cut to be made as have after three joints standbyly as cuttings, and the cuttings upper cut is apart from upper bud 1cm;
Step 2, cuttage are cultivated
Remove cuttings a slice compound leaf bottom, soak the 30-60 min of 1-2cm place, cuttings lower end with root-growing agent, then cuttings being placed in to temperature is 20-25 ℃, standing 12h in the greenhouse that relative moisture is 85-90%, insert cuttings in the triangular flask that matrix is housed and secretly cultivate afterwards, and insertion depth is 3cm, triangular flask is applied to the ultrasonic that frequency is 25kHz simultaneously, the every work 5s of ultrasonic, stop 5～8s, amounts to work 8min; Described matrix is comprised of peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, the volume ratio of each component is 3:1:3:1:0.005:0.005, and wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10
9individual/mL;
Step 3, Room management
Keeping the temperature in greenhouse is 20～30 ℃, relative moisture 75～85%, and cuttings is carried out to intermittent spraying, spray method is: before taking root, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration, the KH that the mass percent concentration of rear every 5 d sprays of taking root is 0.2%
2pO
4the urea liquid that solution and mass percent concentration are 0.2% is to supplement the nutrients;
Step 4, more summer maintenance
Cuttage rooting started hardening after 28～32 days, entered the more maintenance stage in summer after hardening completes, and in greenhouse, spraying weekly is once the solution after 25% carbendazim solution dilutes 500 times by mass percent concentration; Start to carry out axillalry bud after entering more the maintenance stage in summer and induce, during regularly spray composite fertilizer's solution and trace element, and smear kryptoblast bottom with the rudiment agent; Described rudiment agent is fast by 6-benzyl gland, kinetin, gibberellin, glycerine and lanolin form, and the each component proportioning is the fast 0.04%-0.10% of 6-benzyl gland, kinetin 0.01%-0.05%, gibberellin 0.005%-0.02%, methyl-sulfoxide 1.0-3.0%, glycerine 1% and lanolin 94.93%-97.59%;
Step 5, overwinter management
Enter after the winter seedling of taking root and be placed in warm canopy, keep temperature to be not less than 0 ℃, relative moisture is not less than 70%;
Step 6, strong seedling cultivation
The seedling of taking root after Second Year thaws March takes out in container, together with the matrix agglomerate, is transplanted into land for growing field crops, waters once permeablely, plays the high rib insulation of 8～12cm soil moisture conservation.
2. the method for the tender stem cottage propagation of a kind of Chinese herbaceous peony according to claim 1, it is characterized in that: the preparation method of the described matrix of step 2 is: for 3:1:3:1:0.005:0.005 takes peat, perlite, vermiculite, human and animal excreta, hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 * 10 by volume
9individual/mL; Then peat, perlite, vermiculite and human and animal excreta are stirred in proportion, and carry out sterilizing, add azotobacter chroococcum bacterium liquid afterwards in matrix, while prepare using, within first 7 days, add hay bacillus bacterium liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310505898.3A CN103493677B (en) | 2013-10-24 | 2013-10-24 | Method of cutting propagation of peony immature stem |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310505898.3A CN103493677B (en) | 2013-10-24 | 2013-10-24 | Method of cutting propagation of peony immature stem |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103493677A true CN103493677A (en) | 2014-01-08 |
| CN103493677B CN103493677B (en) | 2015-01-28 |
Family
ID=49859022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310505898.3A Active CN103493677B (en) | 2013-10-24 | 2013-10-24 | Method of cutting propagation of peony immature stem |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103493677B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843558A (en) * | 2014-02-20 | 2014-06-11 | 河南省林业学校 | Propagation method of itoh hybrid peony cuttage |
| CN104556388A (en) * | 2014-05-28 | 2015-04-29 | 湖南农业大学 | Method for promoting aquatic plants to remove heavy metals in sediments employing ultrasound |
| CN105165440A (en) * | 2015-08-26 | 2015-12-23 | 扬州大学 | Method for improving mechanical strength of stalks of peony cut flowers |
| CN105210697A (en) * | 2015-08-24 | 2016-01-06 | 扬州大学 | A kind of method suppressing Chinese herbaceous peony collateral development |
| CN105432410A (en) * | 2015-12-08 | 2016-03-30 | 北京农学院 | Peat soil and hokutolite complex culture medium for southern magnolia and preparation method thereof |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN105767012A (en) * | 2016-04-18 | 2016-07-20 | 杭州富阳飞博科技有限公司 | Accelerant facilitating plant cutting rooting |
| CN105815206A (en) * | 2016-05-16 | 2016-08-03 | 张亮 | Vegetable growing system |
| CN106171641A (en) * | 2016-07-27 | 2016-12-07 | 阜南县腾祥工艺品有限公司 | A kind of method for planting improving Pericarpium Citri Reticulatae Viride purple willow quality |
| CN106211968A (en) * | 2016-07-22 | 2016-12-14 | 阜南县永盛工艺品有限公司 | A kind of implantation methods of Vitex chinensis Mill. |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106576714A (en) * | 2016-10-27 | 2017-04-26 | 安徽省霍山县祥佑堂中药材开发有限公司 | Dendrobium huoshanese cutting seedling-raising method |
| CN106613718A (en) * | 2016-11-26 | 2017-05-10 | 阜阳金满地生态农业科技股份有限公司 | Cultivation method for grape cutting seedlings with high cold resistance |
| CN108668657A (en) * | 2018-04-19 | 2018-10-19 | 北京林业大学 | The stem of distant hybridization Cultivars of Chinese Herbaceous Peony inserts propagation method between a kind of group |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN114190187A (en) * | 2021-11-04 | 2022-03-18 | 山东省农业科学院 | Continuous soaking and spraying system after peony is divided into plants |
| CN114885689A (en) * | 2022-04-28 | 2022-08-12 | 四川省农业科学院经济作物育种栽培研究所 | Cultivation method of peony seedlings |
| CN115125176A (en) * | 2022-08-02 | 2022-09-30 | 河南省林业科学研究院 | Composite microbial agent and method for promoting growth and disease resistance of poplar cutting seedlings |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002096185A1 (en) * | 2001-05-23 | 2002-12-05 | Won-Gon Kim | Landscape plant |
| CN101861822A (en) * | 2010-06-07 | 2010-10-20 | 北京林业大学 | Peony cut-flower forcing culture method |
| CN102511488A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Plant sprouting agent and preparation method thereof |
| CN102960222A (en) * | 2012-11-12 | 2013-03-13 | 北京林业大学 | Peony soilless culture industrialization pot flower production method |
| CN103299888A (en) * | 2013-04-27 | 2013-09-18 | 北京林业大学 | Paeonia lactiflora soilless culture substrate and application thereof |
-
2013
- 2013-10-24 CN CN201310505898.3A patent/CN103493677B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002096185A1 (en) * | 2001-05-23 | 2002-12-05 | Won-Gon Kim | Landscape plant |
| CN101861822A (en) * | 2010-06-07 | 2010-10-20 | 北京林业大学 | Peony cut-flower forcing culture method |
| CN102511488A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Plant sprouting agent and preparation method thereof |
| CN102960222A (en) * | 2012-11-12 | 2013-03-13 | 北京林业大学 | Peony soilless culture industrialization pot flower production method |
| CN103299888A (en) * | 2013-04-27 | 2013-09-18 | 北京林业大学 | Paeonia lactiflora soilless culture substrate and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 付喜玲等: "《3个芍药品种茎插生根技术的研究》", 《山东林业科技》 * |
| 张延东，张莉: "《不同芍药品种扦插试验初探》", 《甘肃林业科技》 * |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843558A (en) * | 2014-02-20 | 2014-06-11 | 河南省林业学校 | Propagation method of itoh hybrid peony cuttage |
| CN103843558B (en) * | 2014-02-20 | 2016-01-06 | 河南省林业学校 | A kind of propagation method of her rattan hybrid tree peony cuttage |
| CN104556388A (en) * | 2014-05-28 | 2015-04-29 | 湖南农业大学 | Method for promoting aquatic plants to remove heavy metals in sediments employing ultrasound |
| CN105210697A (en) * | 2015-08-24 | 2016-01-06 | 扬州大学 | A kind of method suppressing Chinese herbaceous peony collateral development |
| CN105165440A (en) * | 2015-08-26 | 2015-12-23 | 扬州大学 | Method for improving mechanical strength of stalks of peony cut flowers |
| CN105432410A (en) * | 2015-12-08 | 2016-03-30 | 北京农学院 | Peat soil and hokutolite complex culture medium for southern magnolia and preparation method thereof |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN105767012A (en) * | 2016-04-18 | 2016-07-20 | 杭州富阳飞博科技有限公司 | Accelerant facilitating plant cutting rooting |
| CN105767012B (en) * | 2016-04-18 | 2018-03-27 | 河南莱泰园林股份有限公司 | A kind of accelerator beneficial to plant cuttage root-taking |
| CN105815206A (en) * | 2016-05-16 | 2016-08-03 | 张亮 | Vegetable growing system |
| CN106211968A (en) * | 2016-07-22 | 2016-12-14 | 阜南县永盛工艺品有限公司 | A kind of implantation methods of Vitex chinensis Mill. |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106171641A (en) * | 2016-07-27 | 2016-12-07 | 阜南县腾祥工艺品有限公司 | A kind of method for planting improving Pericarpium Citri Reticulatae Viride purple willow quality |
| CN106576714A (en) * | 2016-10-27 | 2017-04-26 | 安徽省霍山县祥佑堂中药材开发有限公司 | Dendrobium huoshanese cutting seedling-raising method |
| CN106613718A (en) * | 2016-11-26 | 2017-05-10 | 阜阳金满地生态农业科技股份有限公司 | Cultivation method for grape cutting seedlings with high cold resistance |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN108668657A (en) * | 2018-04-19 | 2018-10-19 | 北京林业大学 | The stem of distant hybridization Cultivars of Chinese Herbaceous Peony inserts propagation method between a kind of group |
| CN114190187A (en) * | 2021-11-04 | 2022-03-18 | 山东省农业科学院 | Continuous soaking and spraying system after peony is divided into plants |
| CN114885689A (en) * | 2022-04-28 | 2022-08-12 | 四川省农业科学院经济作物育种栽培研究所 | Cultivation method of peony seedlings |
| CN114885689B (en) * | 2022-04-28 | 2023-10-17 | 四川省农业科学院经济作物育种栽培研究所 | Cultivation method of paeonia lactiflora seedlings |
| CN115125176A (en) * | 2022-08-02 | 2022-09-30 | 河南省林业科学研究院 | Composite microbial agent and method for promoting growth and disease resistance of poplar cutting seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103493677B (en) | 2015-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103493677B (en) | Method of cutting propagation of peony immature stem | |
| CN103798030B (en) | Anji white tea ecological and efficient cultivation method | |
| CN103283450B (en) | Golden camellia bud seedling stock grafting propagation method | |
| CN104429581B (en) | A kind of Organic tomato health cultural method | |
| CN106233996B (en) | Organic crop rotation cultivation method for early-maturing peppers, rice and water chestnuts | |
| CN105340554A (en) | Planting method of Chinese wolfberries | |
| CN103609413A (en) | Organic ecotype soilless culture technology for Cucurbita pepo L in sunlight greenhouse | |
| CN105917902A (en) | Breeding method for beautiful millettia root | |
| CN104823674A (en) | Muskmelon organic cultivation annual production method | |
| CN101401524A (en) | Cutting propagation method for beach plum epicormic branch | |
| CN103609414A (en) | Organic ecotype soilless culture technology for muskmelons in sunlight greenhouse | |
| CN105766298A (en) | Crop rotation organic cultivation method for summer and autumn watermelons, vegetable broad beans, rice and chufas | |
| CN104756698A (en) | Yield-increase type culture method of polygonum capitatum | |
| CN103329802A (en) | Pollution-free high-yield planting method for strawberries | |
| CN104170656A (en) | Grafting method for thin-skin muskmelons | |
| CN106258484A (en) | A kind of high altitudes and cold area efficient implantation methods of Delicious lactarius | |
| CN103875383A (en) | High-yield cultivation method capable of reducing strawberry malformed fruits | |
| CN106718483A (en) | A kind of apple tree implantation methods | |
| CN107197739A (en) | A kind of cultural method for improving tree peony survival rate | |
| CN105123253B (en) | A kind of method for culturing seedlings of paris polyphylla | |
| CN103274837B (en) | A kind of Polyelement organic wintersweet compound fertilizer | |
| CN107307003A (en) | A kind of Bougainvillea spectabilis root-growing agent and preparation method thereof | |
| CN104823620A (en) | Planting method for green tea | |
| CN103583196A (en) | Method for cultivating greenhouse thin-skin melons | |
| CN103304328A (en) | Multielement organic compound fertilizer for preventing and controlling wintersweet anthracnose |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |