CN106576714A - Dendrobium huoshanese cutting seedling-raising method - Google Patents
Dendrobium huoshanese cutting seedling-raising method Download PDFInfo
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- CN106576714A CN106576714A CN201610952763.5A CN201610952763A CN106576714A CN 106576714 A CN106576714 A CN 106576714A CN 201610952763 A CN201610952763 A CN 201610952763A CN 106576714 A CN106576714 A CN 106576714A
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- cutting
- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a dendrobium huoshanese cutting seedling-raising method, and belongs to the field of planting. Specifically, the method comprises the following steps: (1) preparing cuttings; (2) preparing a steeping liquid; (3) processing the cuttings; (4) performing cuttage processing; (5) managing after germination; (6) transplanting buds. The method is rational in matching of each step, bud ratio of cuttage can be maintained above 96%, budding duration is shortened to about 23 days, transplanting survival rate reaches high up to 98%, and obtained seedlings have better quality and economic benefit is relatively high.
Description
Technical field
The invention belongs to field of planting, and in particular to a kind of Herba Dendrobii cuttage and seedling culture method.
Background technology
Herba Dendrobii is commonly called as a meter dry measure used in former times, is the herbaceous plant of orchid family Dendrobium.China national geography famous special product.Main product is in big
The Huoshan County of Anhui Province in other mountain area, be grown in mostly between the overhanging cliff cliffstone gap that cloud and mist is curled up and old trees that reach into the skies on.Huoshan
Herba Dendrobii can increase substantially SOD in human body（The main matter of slow down aging）Level, Jing is often stayed up late, with brain, tobacco and wine excessively,
The weak crowd of body void, Jing often drinks and is particularly suitable for.Herba Dendrobii has function of improving eyesight, also can YIN and YANG balance regulating, strengthening yang and invigorating kidney, skin care
In appearance, so as to reach effect of health-care.
Herba Dendrobii habitat is unique, and miniclimate is required very strictly, to be frequently grown between 500 ~ 1500m of height above sea level, grows nonparasitically upon another plant
On overhanging cliff or on the trunk of broad leaf tree and on limestone, warm, moistening, the environment of half light are liked.Under wild state,
Herba Dendrobii proliferative speed is low, poor growth, in addition predatory excavation so as to which wild resource is increasingly reduced, and far can not be met
Domestic and international market economic development and clinical medical needs, it is therefore desirable to artificial breeding.
It is first to sprout take root afterwards that cuttage is the characteristics of Herba Dendrobii breeds more conventional means, its cutting propagation, false squama
Stem must first differentiate a complete sprout.After a period of time, sprout base portion differentiates root, is individually formed a planting
Strain, rather than the base portion in cutting forms root system.This characteristic causes Herba Dendrobii cutting propagation speed slow.Existing cuttage side
In method, sprout could sprout for 2 months or so typically after cuttage, and bud ratio is relatively low, the incubation of seedling then needs 4 months left sides
The right side, transplanting survival rate is relatively low, and exactly above-mentioned factor limits the development of Herba Dendrobii industry.Therefore, a kind of educating for high-quality is sought
Seedling-growing method is the problem of those skilled in the art's urgent need to resolve.
The content of the invention
The present invention is intended to provide a kind of Herba Dendrobii cuttage and seedling culture method, with bud ratio height, survival rate height, seedling raise period
Short the advantages of.
The present invention is achieved through the following technical solutions：
A kind of Herba Dendrobii cuttage and seedling culture method, comprises the steps：
（1）It is prepared by cutting：
Best in quality, no disease and pests harm, the spring Herba Dendrobii of robust growth are chosen, selects the full pseudobulb do not bloomed to do cutting, will
It is cut into several sections, and per section has 2 ~ 3 sections, after the completion of be obtained cutting it is standby；
（2）It is prepared by impregnation liquid：
Material being weighed by correspondence weight portion and being configured to impregnation liquid, the percentage by weight of each material is in the impregnation liquid：150~
160ppm auxin, 60 ~ 70ppm gibberellins, 50 ~ 60ppm basic elements of cell division, 20 ~ 30ppm abscisic acid, 0.2 ~ 0.4% magnesium sulfate,
0.2 ~ 0.3% ferrous sulfate, 0.1 ~ 0.2% manganese sulfate, 0.2 ~ 0.4% zinc sulfate, 0.05 ~ 0.15% copper sulfate, 0.2 ~ 0.4% nitric acid
Calcium, 0.3 ~ 0.5% sodium humate, 0.6 ~ 0.8% potassium chloride, 2 ~ 4% glucoses, 1 ~ 2% shitosan, 1 ~ 2% compound vitamin, 0.3 ~
0.5% boric acid, 1 ~ 1.5% compound sodium nitrophenolate, balance of water；
（3）Cutting process：
By step（1）Cutting after process puts into step（2）In obtained impregnation liquid, take out after 30 ~ 40min of immersion treatment,
Then it is drawn off again carrying out a supersound process, processes and take out standby after 3 ~ 4min；
（4）Cuttage is processed：
By step（3）Cutting after process presses the positive upright cuttage of stipes in cutting medium, and depth during cuttage is former cutting
The half of length, keeps the temperature after cuttage to be 19 ~ 24 DEG C, until sprout completing；
（5）Manage after sprouting：
When the bud on cutting differentiates 2 ~ 3 leaf buds, the twice ultrasonic for proceeding by two weeks by a definite date is processed, and one is processed daily
It is secondary, each 3 ~ 4h of continuous processing；
（6）Sprout is transplanted：
By step（5）Single sprout on cutting after process cuts respectively, and is transplanted into the healthy and strong seedling of incubation in nutritive cube i.e.
Can.
Further, step（2）Described in compound vitamin in contain vitamin A, vitamin B2, FA, dimension
Raw element B12, vitamin C, vitamin D, Vitamin E and vitamin K.
Further, step（3）Described in a supersound process frequency be 53 ~ 55kHz.
Further, step（4）Described in cutting medium by Cortex Pini, Pericarppium arachidis hypogaeae, meerschaum and kieselguhr press quality
Than 4：2：2：1 mixing composition.
Further, step（5）Described in twice ultrasonic process frequency be 25 ~ 27kHz.
The present invention has the advantages that：
The present invention has carried out first special process according to the characteristic of Herba Dendrobii cuttage growth before cuttage, wherein prepare
The phytohormone constituents containing several special proportionings, can well regulate and control the germination and growth of cutting sprout, root system in impregnation liquid,
Other nutritional labelings not only can provide the material base of abundance for the development of sprout, while can also improve bud ratio and spy of taking root
Property；The ultrasonic Treatment of applying can break the resting state of stem section, improve the activity of various enzymes, induce the division minute of cutting bud point
Change and the subsequently growth of root system, further improve bud ratio, and shorten the duration that sprouts；The twice ultrasonic carried out after sprouting
Process, can prevent leaf bud from changing to bud, it is to avoid the phenomenon for not forming plantlet and directly blooming after part cutting rudiment
Occur, improve cuttage planting percent, while and the growth promoter of sprout root system can be promoted, it is that survival after transplant is ready.Each
Under the reasonable coordination of step, the bud ratio of cuttage can be maintained at more than 96% by the inventive method, and the duration that sprouts foreshortens to 23 days left sides
The right side, the survival rate of transplanting is up to more than 98%, and more preferably, economic benefit is higher for the seedling quality educated.
Specific embodiment
Embodiment 1
A kind of Herba Dendrobii cuttage and seedling culture method, comprises the steps：
（1）It is prepared by cutting：
Best in quality, no disease and pests harm, the spring Herba Dendrobii of robust growth are chosen, selects the full pseudobulb do not bloomed to do cutting, will
It is cut into several sections, and per section has 2 sections, after the completion of be obtained cutting it is standby；
（2）It is prepared by impregnation liquid：
Material being weighed by correspondence weight portion and being configured to impregnation liquid, the percentage by weight of each material is in the impregnation liquid：150ppm
Auxin, 60ppm gibberellins, the 50ppm basic elements of cell division, 20ppm abscisic acid, 0.2% magnesium sulfate, 0.2% ferrous sulfate, 0.1% sulfur
Sour manganese, 0.2% zinc sulfate, 0.05% copper sulfate, 0.2% calcium nitrate, 0.3% sodium humate, 0.6% potassium chloride, 2% glucose, 1% shell
Polysaccharide, 1% compound vitamin, 0.3% boric acid, 1% compound sodium nitrophenolate, balance of water；
（3）Cutting process：
By step（1）Cutting after process puts into step（2）In obtained impregnation liquid, take out after immersion treatment 30min, then
It is drawn off again carrying out a supersound process, processes and take out standby after 3min；
（4）Cuttage is processed：
By step（3）Cutting after process presses the positive upright cuttage of stipes in cutting medium, and depth during cuttage is former cutting
The half of length, keeps the temperature after cuttage to be 19 ~ 24 DEG C, until sprout completing；
（5）Manage after sprouting：
When the bud on cutting differentiates 2 ~ 3 leaf buds, the twice ultrasonic for proceeding by two weeks by a definite date is processed, and one is processed daily
It is secondary, each continuous processing 3h；
（6）Sprout is transplanted：
By step（5）Single sprout on cutting after process cuts respectively, and is transplanted into the healthy and strong seedling of incubation in nutritive cube i.e.
Can.
Further, step（2）Described in compound vitamin in contain vitamin A, vitamin B2, FA, dimension
Raw element B12, vitamin C, vitamin D, Vitamin E and vitamin K.
Further, step（3）Described in a supersound process frequency be 53kHz.
Further, step（4）Described in cutting medium by Cortex Pini, Pericarppium arachidis hypogaeae, meerschaum and kieselguhr press quality
Than 4：2：2：1 mixing composition.
Further, step（5）Described in twice ultrasonic process frequency be 25kHz.
Embodiment 2
A kind of Herba Dendrobii cuttage and seedling culture method, comprises the steps：
（1）It is prepared by cutting：
Best in quality, no disease and pests harm, the spring Herba Dendrobii of robust growth are chosen, selects the full pseudobulb do not bloomed to do cutting, will
It is cut into several sections, and per section has 3 sections, after the completion of be obtained cutting it is standby；
（2）It is prepared by impregnation liquid：
Material being weighed by correspondence weight portion and being configured to impregnation liquid, the percentage by weight of each material is in the impregnation liquid：155ppm
Auxin, 65ppm gibberellins, the 55ppm basic elements of cell division, 25ppm abscisic acid, 0.3% magnesium sulfate, 0.25% ferrous sulfate, 0.15%
Manganese sulfate, 0.3% zinc sulfate, 0.1% copper sulfate, 0.3% calcium nitrate, 0.4% sodium humate, 0.7% potassium chloride, 3% glucose, 1.5%
Shitosan, 1.5% compound vitamin, 0.4% boric acid, 1.2% compound sodium nitrophenolate, balance of water；
（3）Cutting process：
By step（1）Cutting after process puts into step（2）In obtained impregnation liquid, take out after immersion treatment 35min, then
It is drawn off again carrying out a supersound process, processes and take out standby after 3.5min；
（4）Cuttage is processed：
By step（3）Cutting after process presses the positive upright cuttage of stipes in cutting medium, and depth during cuttage is former cutting
The half of length, keeps the temperature after cuttage to be 19 ~ 24 DEG C, until sprout completing；
（5）Manage after sprouting：
When the bud on cutting differentiates 2 ~ 3 leaf buds, the twice ultrasonic for proceeding by two weeks by a definite date is processed, and one is processed daily
It is secondary, each continuous processing 3.5h；
（6）Sprout is transplanted：
By step（5）Single sprout on cutting after process cuts respectively, and is transplanted into the healthy and strong seedling of incubation in nutritive cube i.e.
Can.
Further, step（2）Described in compound vitamin in contain vitamin A, vitamin B2, FA, dimension
Raw element B12, vitamin C, vitamin D, Vitamin E and vitamin K.
Further, step（3）Described in a supersound process frequency be 54kHz.
Further, step（4）Described in cutting medium by Cortex Pini, Pericarppium arachidis hypogaeae, meerschaum and kieselguhr press quality
Than 4：2：2：1 mixing composition.
Further, step（5）Described in twice ultrasonic process frequency be 26kHz.
Embodiment 3
A kind of Herba Dendrobii cuttage and seedling culture method, comprises the steps：
（1）It is prepared by cutting：
Best in quality, no disease and pests harm, the spring Herba Dendrobii of robust growth are chosen, selects the full pseudobulb do not bloomed to do cutting, will
It is cut into several sections, and per section has 3 sections, after the completion of be obtained cutting it is standby；
（2）It is prepared by impregnation liquid：
Material being weighed by correspondence weight portion and being configured to impregnation liquid, the percentage by weight of each material is in the impregnation liquid： 160ppm
Auxin, 70ppm gibberellins, the 60ppm basic elements of cell division, 30ppm abscisic acid, 0.4% magnesium sulfate, 0.3% ferrous sulfate, 0.2% sulfur
Sour manganese, 0.4% zinc sulfate, 0.15% copper sulfate, 0.4% calcium nitrate, 0.5% sodium humate, 0.8% potassium chloride, 4% glucose, 2% shell
Polysaccharide, 2% compound vitamin, 0.5% boric acid, 1.5% compound sodium nitrophenolate, balance of water；
（3）Cutting process：
By step（1）Cutting after process puts into step（2）In obtained impregnation liquid, take out after immersion treatment 40min, then
It is drawn off again carrying out a supersound process, processes and take out standby after 4min；
（4）Cuttage is processed：
By step（3）Cutting after process presses the positive upright cuttage of stipes in cutting medium, and depth during cuttage is former cutting
The half of length, keeps the temperature after cuttage to be 19 ~ 24 DEG C, until sprout completing；
（5）Manage after sprouting：
When the bud on cutting differentiates 2 ~ 3 leaf buds, the twice ultrasonic for proceeding by two weeks by a definite date is processed, and one is processed daily
It is secondary, each continuous processing 4h；
（6）Sprout is transplanted：
By step（5）Single sprout on cutting after process cuts respectively, and is transplanted into the healthy and strong seedling of incubation in nutritive cube i.e.
Can.
Further, step（2）Described in compound vitamin in contain vitamin A, vitamin B2, FA, dimension
Raw element B12, vitamin C, vitamin D, Vitamin E and vitamin K.
Further, step（3）Described in a supersound process frequency be 55kHz.
Further, step（4）Described in cutting medium by Cortex Pini, Pericarppium arachidis hypogaeae, meerschaum and kieselguhr press quality
Than 4：2：2：1 mixing composition.
Further, step（5）Described in twice ultrasonic process frequency be 27kHz.
Comparative example 1
This comparative example 1 compared with Example 1, does not contain gibberellins, the basic element of cell division and abscisic acid composition in its impregnation liquid,
With etc. the auxin of mass parts replace, method and step all same in addition.
Comparative example 2
This comparative example 2 compared with Example 2, in step（3）During cutting process, a ultrasonic Treatment operation is not carried out,
Method and step all same in addition.
Comparative example 3
This comparative example 3 carries out compared with Example 3, not step（5）Management operation after sprouting, method and step in addition is equal
It is identical.
Matched group
Existing Herba Dendrobii cuttage and seedling culture method.
In order to contrast effect of the present invention, from cutting is made with a batch of Herba Dendrobii maternal plant, respectively with above-mentioned seven kinds
Mode is cultivated, and the growth data of each group during counting cutting propagation, table 1 below is corresponding correction data：
Table 1
By upper table 1 as can be seen that the inventive method can well improve bud ratio, shorten the duration that sprouts, educate sprout entirety product
Matter is good, and transplanting survival rate is high, it is ensured that the yield of cutting seedling, improves planting benefit.
In order to further contrast effect of the present invention, the Herba Dendrobii Seedling after cultivating 4 months above-mentioned seven kinds of methods is surveyed
Amount, concrete correction data is as shown in table 2 below：
Table 2
| Plant height（cm） | Take root number（Bar） | Root is long（cm） | |
| Embodiment 1 | 9.62 | 5.6 | 3.22 |
| Embodiment 2 | 9.71 | 5.8 | 3.31 |
| Embodiment 3 | 9.66 | 5.5 | 3.24 |
| Comparative example 1 | 9.21 | 5.1 | 3.10 |
| Comparative example 2 | 8.72 | 4.7 | 2.88 |
| Comparative example 3 | 8.54 | 4.6 | 2.76 |
| Matched group | 7.35 | 4.2 | 2.52 |
By upper table 2 as can be seen that the seedling quality educated within the same period of method for culturing seedlings of the present invention more preferably, characteristic is more preferable, great city
Field competitiveness.
Claims (5)
1. a kind of Herba Dendrobii cuttage and seedling culture method, it is characterised in that comprise the steps：
（1）It is prepared by cutting：
Best in quality, no disease and pests harm, the spring Herba Dendrobii of robust growth are chosen, selects the full pseudobulb do not bloomed to do cutting, will
It is cut into several sections, and per section has 2 ~ 3 sections, after the completion of be obtained cutting it is standby；
（2）It is prepared by impregnation liquid：
Material being weighed by correspondence weight portion and being configured to impregnation liquid, the percentage by weight of each material is in the impregnation liquid：150~
160ppm auxin, 60 ~ 70ppm gibberellins, 50 ~ 60ppm basic elements of cell division, 20 ~ 30ppm abscisic acid, 0.2 ~ 0.4% magnesium sulfate,
0.2 ~ 0.3% ferrous sulfate, 0.1 ~ 0.2% manganese sulfate, 0.2 ~ 0.4% zinc sulfate, 0.05 ~ 0.15% copper sulfate, 0.2 ~ 0.4% nitric acid
Calcium, 0.3 ~ 0.5% sodium humate, 0.6 ~ 0.8% potassium chloride, 2 ~ 4% glucoses, 1 ~ 2% shitosan, 1 ~ 2% compound vitamin, 0.3 ~
0.5% boric acid, 1 ~ 1.5% compound sodium nitrophenolate, balance of water；
（3）Cutting process：
By step（1）Cutting after process puts into step（2）In obtained impregnation liquid, take out after 30 ~ 40min of immersion treatment,
Then it is drawn off again carrying out a supersound process, processes and take out standby after 3 ~ 4min；
（4）Cuttage is processed：
By step（3）Cutting after process presses the positive upright cuttage of stipes in cutting medium, and depth during cuttage is former cutting
The half of length, keeps the temperature after cuttage to be 19 ~ 24 DEG C, until sprout completing；
（5）Manage after sprouting：
When the bud on cutting differentiates 2 ~ 3 leaf buds, the twice ultrasonic for proceeding by two weeks by a definite date is processed, and one is processed daily
It is secondary, each 3 ~ 4h of continuous processing；
（6）Sprout is transplanted：
By step（5）Single sprout on cutting after process cuts respectively, and is transplanted into the healthy and strong seedling of incubation in nutritive cube i.e.
Can.
2. a kind of Herba Dendrobii cuttage and seedling culture method according to claim 1, it is characterised in that step（2）Described in
In compound vitamin containing vitamin A, vitamin B2, FA, Vitamin B12, vitamin C, vitamin D, Vitamin E and
Vitamin K.
3. a kind of Herba Dendrobii cuttage and seedling culture method according to claim 1, it is characterised in that step（3）Described in
The frequency of supersound process is 53 ~ 55kHz.
4. a kind of Herba Dendrobii cuttage and seedling culture method according to claim 1, it is characterised in that step（4）Described in
Cutting medium is by Cortex Pini, Pericarppium arachidis hypogaeae, meerschaum and kieselguhr in mass ratio 4：2：2：1 mixing composition.
5. a kind of Herba Dendrobii cuttage and seedling culture method according to claim 1, it is characterised in that step（5）Described in
The frequency of twice ultrasonic process is 25 ~ 27kHz.
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| CN201610952763.5A CN106576714A (en) | 2016-10-27 | 2016-10-27 | Dendrobium huoshanese cutting seedling-raising method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431128A (en) * | 2022-02-28 | 2022-05-06 | 广州市林业和园林科学研究院 | Method for promoting rooting of ornamental dendrobium aduncum |
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|---|---|---|---|---|
| CN103314740A (en) * | 2013-05-24 | 2013-09-25 | 安徽中升生物科技有限公司 | Method for planting dendrobium huoshanense seedlings in cottage mode |
| CN103493677A (en) * | 2013-10-24 | 2014-01-08 | 河南科技大学 | Method of cutting propagation of peony immature stem |
| CN106008030A (en) * | 2016-07-10 | 2016-10-12 | 合肥扬扬农业科技有限公司 | Tree nutrient solution |
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2016
- 2016-10-27 CN CN201610952763.5A patent/CN106576714A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314740A (en) * | 2013-05-24 | 2013-09-25 | 安徽中升生物科技有限公司 | Method for planting dendrobium huoshanense seedlings in cottage mode |
| CN103493677A (en) * | 2013-10-24 | 2014-01-08 | 河南科技大学 | Method of cutting propagation of peony immature stem |
| CN106008030A (en) * | 2016-07-10 | 2016-10-12 | 合肥扬扬农业科技有限公司 | Tree nutrient solution |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114431128A (en) * | 2022-02-28 | 2022-05-06 | 广州市林业和园林科学研究院 | Method for promoting rooting of ornamental dendrobium aduncum |
| CN114431128B (en) * | 2022-02-28 | 2023-09-12 | 广州市林业和园林科学研究院 | Method for promoting rooting of ornamental dendrobium attached trees |
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