CN105532294A - Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese - Google Patents
Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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Abstract
Provided is a cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese. The method is characterized by comprising the following steps: acquiring 2-5 years old thick branches and making the branches into cuttings, after standing completes, using NAA+2,4-D mixed liquor to soak 0-4 cm of cutting bases, while soaking, using an ultrasonic cell crusher to perform ultrasonic treatment on the cuttings, establishing a full-automatic mist-spraying cutting pool, paving a medium which is disinfected in the cutting pool, putting the cuttings in the medium, after cuttage, using a watering can to watering, to make the cuttings tightly contact with the medium, and performing management after cuttage.
Description
Technical field
The present invention relates to a kind of cultivation method, particularly a kind of cuttage breeding method improving Chinese tulip tree rooting percent.
Background technology
The preservation of Chinese tulip tree resource and the fast breeding of seedling are the bases solving the construction of taxol timber forest, and it is the crux solving taxol raw material that large area builds aged artificial Liriodendron chinense.The poky seeds of Liriodendron, be again dioecian plant, seed-setting amount is little, and kind embryo after-ripening resting stage is long, there is seed sprouting irregular, the problems such as germination rate is not high.And the development of the recent raw material forest of southern Chinese tulip tree, be to be utilized as strategy the seedling stage of intensive plantation, therefore take the modes of reproduction of cuttage in enormous quantities to be the basis realizing this strategy.
The object of this invention is to provide a kind of cuttage breeding method improving Chinese tulip tree rooting percent, that can improve seedling breeds speed, reduce production cost, and survival rate is high.
Summary of the invention
For achieving the above object, the technical solution used in the present invention is:
Improve a cuttage breeding method for Chinese tulip tree rooting percent, gather the raw sturdy branch of 2-5 and carry out fringe processed, cutting length is 5-10cm, stays leaf 3-4 sheet; Cuttings being placed in temperature is 20-25 DEG C, relative moisture be 85-90% greenhouse in leave standstill 12h, cuttings is inserted in the triangular flask that liquid is housed afterwards and carry out light culture, insertion depth is 3cm, apply frequency to triangular flask is the ultrasonic of 25kHz simultaneously, ultrasonic often works 5s, stops 5 ~ 8s, amounts to work 8min; Described liquid is mixed by benzyladenine 0.2wt%, plant Vc 0.1wt%, boric acid 0.3wt%, citric acid 0.15wt%, water;
Leave standstill after terminating and adopt NAA+2,4-D mixed liquor carries out immersion treatment to cuttings base portion 0-4cm, carries out ultrasonic process while immersion with ultrasonic cell disruption instrument to cuttings; The power of described ultrasonic cell disruption instrument is 100-150w; Described ultrasonic process comprises: each ultrasonic 5-7s, the interval 5-7s of adjacent twice ultrasonic process, and the total time of ultrasonic process is 2-3min,
Set up full-automatic spraying cuttage pond, the matrix through disinfecting is laid in cuttage pond, matrix adopts volume ratio to be peat soil: perlite: hay bacillus bacterium liquid: the mixing matrix of azotobacter chroococcum bacterium liquid composition=1: 1: 0.005: 0.005, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 × 109/mL, and laying depth is 10-12cm; By cuttings cuttage to Medium Culture; The cuttings cuttage degree of depth is 3-4cm, and density seeding row spacing is 4cm × 5cm, waters after cuttage completes with water sprager, makes cuttings and matrix close contact, enters the management after cuttage afterwards;
First 30 days after cuttage, the temperature controlling matrix was 25 DEG C-28 DEG C, water content of substrate 50%-70%; And intermittent spraying is carried out to cuttings, spray method is: spraying weekly before taking root is once the solution that 25% carbendazim solution dilutes after 500 times by mass percent concentration, after taking root every 5d spray mass percent concentration be 0.2% KH2PO4 solution and mass percent concentration be that the urea liquid of 0.2% is to supplement the nutrients;
Rooting and transplant: have the cuttage seeding of 60% to take root, cuttage seeding is transplanted, start to enter the hardening phase when rooting percent reaches 75-90%, 10-15 days hardening time got final product field planting.
Sterilization in the 7th day 1 time after cuttage, within every 7-10 days later, the concentration of NAA is the concentration of 1000-2000ppm, 2,4-D is 200ppm-600ppm to seedling raising environment and cuttage is good nursery stock sterilization 1 time thus pre-disease prevention, wherein bactericide employing carbendazim.
A kind of cuttage breeding method improving Chinese tulip tree rooting percent provided is provided, under control matrix has the condition of specific temperature and water content, be aided with specific ultrasonic processing method under the functions of hormones of employing root-inducing powder simultaneously, can greatly divide by irritation cell, not only significantly reduce the time of taking root, also considerably improve rooting rate simultaneously, its ability of withstanding natural calamities and growth promoting effects can also be improved, there is the features such as high, the simple to operate practicality of rooting rate, cost be low, seedling raise period is short, is applicable to extensive cottage propagation.
In addition, for concrete ultrasonic way choice, each ultrasonic time is long, and each sonication times interval is shorter, stimulates, easily cause the destruction to cell while adding root-inducing powder hormone; Each ultrasonic time is too short, each hyperacoustic time interval is longer, ultrasonic does not have the fissional effect of ultrasonic stimulation together with root-inducing powder hormone, and the ultrasonic frequency that the present invention selects, sonication times and ultrasonic interval, the cell division of cuttings can be stimulated to greatest extent, be conducive to root-inducing powder hormone and enter cell interior, impel the formation layer in branch and bundle sheath tissue, form root initial body, thus development growth goes out adventive root, and form root system, improve rooting rate.
Compared with prior art, beneficial effect of the present invention is:
Cuttage breeding method provided by the invention, has that practicality simple to operate, cost are low, rooting rate high, and seedling raise period is short, is applicable to extensive cottage propagation; Ensure that cuttings is germinateed after taking root simultaneously, be conducive to the growth of root system, impel educated seedling root luxuriant.
Embodiment
Embodiment 1
Improve a cuttage breeding method for Chinese tulip tree rooting percent, gather 2 years sturdy branches of life and carry out fringe processed, cutting length is 5cm, stays 3, leaf; Cuttings being placed in temperature is 20 DEG C, relative moisture be 90% greenhouse in leave standstill 12h, cuttings is inserted in the triangular flask that liquid is housed afterwards and carry out light culture, insertion depth is 3cm, apply frequency to triangular flask is the ultrasonic of 25kHz simultaneously, ultrasonic often works 5s, stops 5s, amounts to work 8min; Described liquid is mixed by benzyladenine 0.2wt%, plant Vc 0.1wt%, boric acid 0.3wt%, citric acid 0.15wt%, water;
Leave standstill after terminating and adopt NAA+2,4-D mixed liquor carries out immersion treatment to cuttings base portion 4cm, carries out ultrasonic process while immersion with ultrasonic cell disruption instrument to cuttings; The power of described ultrasonic cell disruption instrument is 100w; Described ultrasonic process comprises: each ultrasonic 5-7s, the interval 5-7s of adjacent twice ultrasonic process, and the total time of ultrasonic process is 2min,
Set up full-automatic spraying cuttage pond, the matrix through disinfecting is laid in cuttage pond, matrix adopts volume ratio to be peat soil: perlite: hay bacillus bacterium liquid: the mixing matrix of azotobacter chroococcum bacterium liquid composition=1: 1: 0.005: 0.005, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 × 10
9individual/mL, laying depth is 10-12cm; By cuttings cuttage to Medium Culture; The cuttings cuttage degree of depth is 3-4cm, and density seeding row spacing is 4cm × 5cm, waters after cuttage completes with water sprager, makes cuttings and matrix close contact, enters the management after cuttage afterwards;
First 30 days after cuttage, the temperature controlling matrix was 25 DEG C-28 DEG C, water content of substrate 50%-70%; And intermittent spraying is carried out to cuttings, spray method is: spraying weekly before taking root is once the solution that 25% carbendazim solution dilutes after 500 times by mass percent concentration, after taking root every 5d spray mass percent concentration be 0.2% KH2PO4 solution and mass percent concentration be that the urea liquid of 0.2% is to supplement the nutrients;
Rooting and transplant: have the cuttage seeding of 60% to take root, cuttage seeding is transplanted, start to enter the hardening phase when rooting percent reaches 75-90%, 10-15 days hardening time got final product field planting.
Sterilization in the 7th day 1 time after cuttage, within every 7-10 days later, the concentration of NAA is the concentration of 1000ppm, 2,4-D is 200ppmppm to seedling raising environment and cuttage is good nursery stock sterilization 1 time thus pre-disease prevention, wherein bactericide employing carbendazim.
Embodiment 2
The present embodiment adopts the method for embodiment 1, and the difference of embodiment 1 is: the power of described ultrasonic cell disruption instrument is 100-150w; Ultrasonic often works 5s, stops 6s, amounts to work 8min; , the total time of ultrasonic process is 4min.The concentration of NAA is the concentration of 2000ppm, 2,4-D is 600ppm.
Comparative group 1-2 adopts the rooting method of the embodiment 1-2 corresponding with its numbering respectively, and difference is that the sample in comparative group does not all adopt ultrasonic process.Clear water control group adopts the rooting method of embodiment 1, and difference is: do not adopt root-inducing powder treatment fluid to soak, and adopts clear water to soak 6h, adopts ultrasonic process and the rooting rate after not adopting ultrasonic process to be 0.
Table 1 hormon and ultrasonic process are on the impact of pecan tree cutting plantation
The rooting rate of embodiment | The rooting rate of comparative example | |
1 | 86.33% | 57.0% |
2 | 91.67% | 58.33% |
Claims (3)
1. improve a cuttage breeding method for Chinese tulip tree rooting percent, it is characterized in that, comprise the steps:
(1) gather the raw sturdy branch of 2-5 and carry out fringe processed, cutting length is 5-10cm, stays leaf 3-4 sheet; Cuttings being placed in temperature is 20-25 DEG C, relative moisture be 85-90% greenhouse in leave standstill 12h, cuttings is inserted in the triangular flask that liquid is housed afterwards and carry out light culture, insertion depth is 3cm, apply frequency to triangular flask is the ultrasonic of 25kHz simultaneously, ultrasonic often works 5s, stops 5 ~ 8s, amounts to work 8min; Described liquid is formed by the mixed preparing that added water by yellow soil, plant growth promoter, bactericide, PH=5 ~ 6.5 of described medicine slurry, described plant growth promoter is mixed by benzyladenine 0.2wt%, plant Vc 0.1wt%, boric acid 0.3wt%, citric acid 0.15wt%, water;
(2) adopt NAA+2 after leaving standstill end, 4-D mixed liquor carries out immersion treatment to cuttings base portion 0-4cm, carries out ultrasonic process while immersion with ultrasonic cell disruption instrument to cuttings; The power of described ultrasonic cell disruption instrument is 100-150w; Described ultrasonic process comprises: each ultrasonic 5-7s, the interval 5-7s of adjacent twice ultrasonic process, and the total time of ultrasonic process is 2-3min,
(3) full-automatic spraying cuttage pond is set up, the matrix through disinfecting is laid in cuttage pond, matrix adopts volume ratio to be peat soil: perlite: hay bacillus bacterium liquid: the mixing matrix of azotobacter chroococcum bacterium liquid composition=1: 1: 0.005: 0.005, wherein the concentration of hay bacillus bacterium liquid and azotobacter chroococcum bacterium liquid is 2.4 × 10
9individual/mL, laying depth is 10-12cm; By cuttings cuttage to Medium Culture; The cuttings cuttage degree of depth is 3-4cm, and density seeding row spacing is 4cm × 5cm, waters after cuttage completes with water sprager, makes cuttings and matrix close contact, enters the management after cuttage afterwards;
(4) first 30 days after cuttage, the temperature controlling matrix was 25 DEG C-28 DEG C, water content of substrate 50%-70%; And intermittent spraying is carried out to cuttings, spray method is: spraying weekly before taking root is once the solution that 25% carbendazim solution dilutes after 500 times by mass percent concentration, and after taking root, every 5d sprays a mass percent concentration is the KH of 0.2%
2pO
4solution and mass percent concentration are that the urea liquid of 0.2% is to supplement the nutrients;
(5) rooting and transplant: have the cuttage seeding of 60% to take root, cuttage seeding is transplanted, start to enter the hardening phase when rooting percent reaches 75-90%, 10-15 days hardening time got final product field planting.
2. the cuttage breeding method improving Chinese tulip tree rooting percent as claimed in claim 1, it is characterized in that: wherein sterilization in the 7th day 1 time after cuttage, every 7-10 days later to seedling raising environment and cuttage is good nursery stock sterilization 1 time thus pre-disease prevention, wherein bactericide employing carbendazim.
3. the as claimed in claim 1 cuttage breeding method improving Chinese tulip tree rooting percent, is characterized in that: the concentration of NAA is the concentration of 1000-2000ppm, 2,4-D is 200ppm-600ppm.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105993522A (en) * | 2016-05-31 | 2016-10-12 | 宜宾云辰乔木园林有限责任公司 | Method for improving tulip tree cutting rooting survival rate |
CN106376607A (en) * | 2016-08-31 | 2017-02-08 | 山东胜伟园林科技有限公司 | Saline land original soil planting method for planting paulownia by employing rooting agent containing sophora bud extract product |
CN107087493A (en) * | 2017-06-15 | 2017-08-25 | 潍坊职业学院 | A kind of golden-rimmed Liriodendron hardwood cuttage method in U.S. |
CN115125176A (en) * | 2022-08-02 | 2022-09-30 | 河南省林业科学研究院 | Composite microbial agent and method for promoting growth and disease resistance of poplar cutting seedlings |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105993522A (en) * | 2016-05-31 | 2016-10-12 | 宜宾云辰乔木园林有限责任公司 | Method for improving tulip tree cutting rooting survival rate |
CN106376607A (en) * | 2016-08-31 | 2017-02-08 | 山东胜伟园林科技有限公司 | Saline land original soil planting method for planting paulownia by employing rooting agent containing sophora bud extract product |
CN107087493A (en) * | 2017-06-15 | 2017-08-25 | 潍坊职业学院 | A kind of golden-rimmed Liriodendron hardwood cuttage method in U.S. |
CN115125176A (en) * | 2022-08-02 | 2022-09-30 | 河南省林业科学研究院 | Composite microbial agent and method for promoting growth and disease resistance of poplar cutting seedlings |
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