CN108781805A - A kind of cuttage processing method improving safflower oil tea cuttage radication capability - Google Patents
A kind of cuttage processing method improving safflower oil tea cuttage radication capability Download PDFInfo
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- CN108781805A CN108781805A CN201810321375.6A CN201810321375A CN108781805A CN 108781805 A CN108781805 A CN 108781805A CN 201810321375 A CN201810321375 A CN 201810321375A CN 108781805 A CN108781805 A CN 108781805A
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- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
Abstract
The invention belongs to cuttage technique field, especially a kind of cuttage processing method improving safflower oil tea cuttage radication capability, the specific method is as follows：（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2 blades, is immersed in purslane extract and impregnates and be passed through ultrasonic wave and be ultrasonically treated, filtrate is pulled out；（2）Steam treatment is taken out, cooling treatment；（3）Magnetization treatment takes out, is immersed in roots soaking agent and impregnates, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；The roots soaking agent is made of following raw material：Barium metaborate, citric acid chelated iron, curcumin, momordica grosvenori polysaccharide, water；The effectively cellular metabolic activity of enhancing cuttage item, enhances the Absorption And Transportation conversion capability to nutriment, and the generation rate of raising cuttage callus and root system promotes cell quickly division and increase, breaks through cortex, promotes root restriction development and the growth of adventitious root elongation.
Description
Technical field
The invention belongs to cuttage technique field, especially a kind of cuttage item processing improving safflower oil tea cuttage radication capability
Method.
Background technology
Safflower oil tea belongs to Theaceae Camellia Section Camellia evergreen shrubs or dungarunga, is China's endemic tree, safflower oil
The fruit of tea is big, and tea seed oil content is high, is high quality food oil tannin plant, at present its area and the yield position in China oil tea is cultivated
Occupy the 4th；Safflower oil tea winter or early spring bloom, and flower is larger, and pattern is red gorgeous, and landscape planting seeds well, still
Safflower oil tea vegetative propagation technique is ripe not as good as C. olelfera, causes its cuttage radication capability weak, undesirable so as to cause nursery；
In addition, the cuttage radication capability in different month safflower oil tea cuttage items is different, especially in safflower oil tea cuttage item in July
Rootability it is weaker, cause rooting rate relatively low, in turn result in that nursery stock is small and weak, and survival rate is low.
Invention content
In view of the above-mentioned problems, the present invention is intended to provide a kind of cuttage item processing side for improving safflower oil tea cuttage radication capability
Method.
The invention is realized by the following technical scheme：
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, the specific method is as follows：
（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2
Blade is immersed in purslane extract and impregnates 66 ~ 74min and be passed through ultrasonic wave and be ultrasonically treated, and filtrate is pulled out, and single treatment is obtained
Cuttage item；
（2）Constant temperature steam handles 6 ~ 7min under conditions of being 48 ~ 54 DEG C by single treatment cuttage item merging temperature, takes out, merging
Cooling treatment is carried out in cooler bin, obtains after-treatment cuttage item；
（3）After-treatment cuttage item is placed in magnetic field and carries out magnetization treatment, takes out, 3.4 ~ 3.8cm of lower end is immersed in leaching root
32 ~ 38min is impregnated in agent, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；
The roots soaking agent is made of the raw material of following parts by weight：0.82 ~ 0.88 part of barium metaborate, citric acid chelated iron 2.1 ~
2.5 parts, 0.23 ~ 0.27 part of curcumin, 0.31 ~ 0.35 part of momordica grosvenori polysaccharide, 170 ~ 190 parts of water.
As invention further embodiment：Step（1）The purslane extract, is prepared according to the following steps：By 43 ~ 45
The purslane of parts by weight crushes, and constant temperature steam handles 25 ~ 30min in the steam box that merging temperature is 210 ~ 230 DEG C, takes out, and is added
18 ~ 20 parts by weight, the ethanol solution that mass fraction is 74% ~ 78% are mixed evenly, the fermenting case that merging temperature is 38 ~ 40 DEG C
Interior 80 ~ 90h of ferment at constant temperature takes out, puts into the water of 120 ~ 130 parts by weight and be boiled by fire, and takes out, cooling, and filtering takes filter
Liquid, the urea that 0.7 ~ 0.8 parts by weight are added are stirred to dissolving, obtain purslane extract.
As invention further embodiment：Step（1）The supersound process is 161 ~ 177W, frequency 119 in power
23 ~ 25min is ultrasonically treated under conditions of ~ 125kHz, pause 14 ~ 18min, power is 103 ~ 115W, frequency is 81 ~ 87kHz
Under conditions of be ultrasonically treated 29 ~ 31min.
As invention further embodiment：Step（2）The cooling treatment, under conditions of temperature is 0.8 ~ 1.4 DEG C
Constant temperature 8 ~ 9min of cooling treatment is warming up to 4.3 ~ 4.9 DEG C and continues 17 ~ 19min of constant temperature cooling treatment.
As invention further embodiment：Step（3）The magnetization treatment, in the condition that magnetic field intensity is 640 ~ 680G
200 ~ 220s of lower magnetization treatment, pause 50 ~ 60s, 330 ~ 350s of magnetization treatment under conditions of magnetic field intensity is 1830 ~ 1890G,
Pause 35 ~ 45s, 160 ~ 180s of magnetization treatment under conditions of magnetic field intensity is 1140 ~ 1180G.
Beneficial effects of the present invention：A kind of cuttage item processing improving safflower oil tea cuttage radication capability provided by the invention
Method uses purslane extract to impregnate cuttage item and combines supersound process first, not only the cellular metabolic activity of effective enhancing cuttage item,
Enhance the Absorption And Transportation conversion capability to nutriment, and effectively improves indoleacetic acid oxidase activity, endogenous indoles of degrading
Acetic acid, the inherent plasmid for adjusting cuttage item is horizontal, so that inherent plasmid is in preferred concentration, is conducive to luring for root restriction
Activation is led, the differentiation of callus and root system is conducive to, improves the generation rate of cuttage callus and root system；Then to cuttage item
Steam treatment and cooling are carried out, the further maturation of cuttage item is promoted, enhances cellular metabolic activity, is conducive to the nutrition of branches and leaves piece
Substance is transported to basal part of stem, and stimulation is taken root, and can effectively enhance the anti-adversity ability of cuttage item, effectively adapts to extraneous bad ring
Border；In the formation and rooting process of adventitious root primordia, needs to consume a large number of nutrients, magnetization treatment finally is carried out to cuttage item
It is used in combination roots soaking agent to carry out leaching root processing, the nutriment for effectively facilitating roots soaking agent penetrates into inside cuttage item, makes it by cuttage item
Metabolism is consumed or is established for the formation of callus and adventitious root as the energy reserve of substance by histoorgan dynamic accumulation
Abundant material base is determined, has promoted cell quickly division and increase, break through cortex, promote root restriction development and the life of adventitious root
Long elongation so that nursery stock is strong.
Specific implementation mode
Illustrate the present invention with specific embodiment below, but is not limitation of the present invention.
Embodiment 1
In the embodiment of the present invention, a kind of cuttage processing method improving safflower oil tea cuttage radication capability, the specific method is as follows：
（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2
Blade is immersed in purslane extract and impregnates 66min and be passed through ultrasonic wave and be ultrasonically treated, and filtrate is pulled out, and single treatment skewer is obtained
Cutting；
（2）Constant temperature steam handles 6min under conditions of being 48 DEG C by single treatment cuttage item merging temperature, takes out, is placed in cooler bin
Interior carry out cooling treatment, obtains after-treatment cuttage item；
（3）After-treatment cuttage item is placed in magnetic field and carries out magnetization treatment, takes out, 3.4 ~ 3.8cm of lower end is immersed in leaching root
32 ~ 38min is impregnated in agent, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；
The roots soaking agent is made of the raw material of following parts by weight：0.82 part of barium metaborate, 2.1 parts of citric acid chelated iron, turmeric
0.23 part of element, 0.31 part of momordica grosvenori polysaccharide, 170 parts of water.
As invention further embodiment：Step（1）The purslane extract, is prepared according to the following steps：By 43 weights
The purslane crushing of part is measured, constant temperature steam handles 25min in the steam box that merging temperature is 210 DEG C, takes out, 18 parts by weight of addition,
The ethanol solution that mass fraction is 74% is mixed evenly, and ferment at constant temperature 80h in the fermenting case that merging temperature is 38 DEG C takes out,
Put into the water of 120 parts by weight and be boiled by fire, take out, cooling, filtering takes filtrate, be added 0.7 parts by weight urea stir to
Dissolving, obtains purslane extract.
As invention further embodiment：Step（1）The supersound process, power be 161W, frequency 119kHz
Under conditions of be ultrasonically treated 23min, pause 14min is ultrasonically treated under conditions of power is 103W, frequency is 81kHz
29min。
As invention further embodiment：Step（2）The cooling treatment, constant temperature under conditions of temperature is 0.8 DEG C
Cooling treatment 8min is warming up to 4.3 DEG C and continues constant temperature cooling treatment 17min.
As invention further embodiment：Step（3）The magnetization treatment, magnetic under conditions of magnetic field intensity is 640G
Change processing 200s, pause 50s, magnetization treatment 330s, pause 35s under conditions of magnetic field intensity is 1830G are in magnetic field intensity
Magnetization treatment 160s under conditions of 1140G.
Embodiment 2
In the embodiment of the present invention, a kind of cuttage processing method improving safflower oil tea cuttage radication capability, the specific method is as follows：
（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2
Blade is immersed in purslane extract and impregnates 70min and be passed through ultrasonic wave and be ultrasonically treated, and filtrate is pulled out, and single treatment skewer is obtained
Cutting；
（2）Constant temperature steam handles 6.5min under conditions of being 51 DEG C by single treatment cuttage item merging temperature, takes out, merging cooling
Cooling treatment is carried out in case, obtains after-treatment cuttage item；
（3）After-treatment cuttage item is placed in magnetic field and carries out magnetization treatment, takes out, 3.4 ~ 3.8cm of lower end is immersed in leaching root
35min is impregnated in agent, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；
The roots soaking agent is made of the raw material of following parts by weight：0.85 part of barium metaborate, 2.3 parts of citric acid chelated iron, turmeric
0.25 part of element, 0.33 part of momordica grosvenori polysaccharide, 180 parts of water.
As invention further embodiment：Step（1）The purslane extract, is prepared according to the following steps：By 44 weights
The purslane crushing of part is measured, constant temperature steam handles 28min in the steam box that merging temperature is 220 DEG C, takes out, 19 parts by weight of addition,
The ethanol solution that mass fraction is 76% is mixed evenly, and ferment at constant temperature 85h in the fermenting case that merging temperature is 39 DEG C takes out,
It puts into the water of 125 parts by weight and is boiled by fire, take out, cooling, filtering takes filtrate, and the urea stirring of 0.75 parts by weight is added
To dissolving, purslane extract is obtained.
As invention further embodiment：Step（1）The supersound process, power be 169W, frequency 122kHz
Under conditions of be ultrasonically treated 24min, pause 16min is ultrasonically treated under conditions of power is 109W, frequency is 84kHz
30min。
As invention further embodiment：Step（2）The cooling treatment, constant temperature under conditions of temperature is 1.1 DEG C
Cooling treatment 8.5min is warming up to 4.6 DEG C and continues constant temperature cooling treatment 18min.
As invention further embodiment：Step（3）The magnetization treatment, magnetic under conditions of magnetic field intensity is 660G
Change processing 210s, pause 55s, magnetization treatment 340s, pause 40s under conditions of magnetic field intensity is 1860G are in magnetic field intensity
Magnetization treatment 170s under conditions of 1160G.
Embodiment 3
In the embodiment of the present invention, a kind of cuttage processing method improving safflower oil tea cuttage radication capability, the specific method is as follows：
（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2
Blade is immersed in purslane extract and impregnates 74min and be passed through ultrasonic wave and be ultrasonically treated, and filtrate is pulled out, and single treatment skewer is obtained
Cutting；
（2）Constant temperature steam handles 7min under conditions of being 54 DEG C by single treatment cuttage item merging temperature, takes out, is placed in cooler bin
Interior carry out cooling treatment, obtains after-treatment cuttage item；
（3）After-treatment cuttage item is placed in magnetic field and carries out magnetization treatment, takes out, 3.4 ~ 3.8cm of lower end is immersed in leaching root
38min is impregnated in agent, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；
The roots soaking agent is made of the raw material of following parts by weight：0.88 part of barium metaborate, 2.5 parts of citric acid chelated iron, turmeric
0.27 part of element, 0.35 part of momordica grosvenori polysaccharide, 190 parts of water.
As invention further embodiment：Step（1）The purslane extract, is prepared according to the following steps：By 45 weights
The purslane crushing of part is measured, constant temperature steam handles 30min in the steam box that merging temperature is 230 DEG C, takes out, 20 parts by weight of addition,
The ethanol solution that mass fraction is 78% is mixed evenly, and ferment at constant temperature 90h in the fermenting case that merging temperature is 40 DEG C takes out,
Put into the water of 130 parts by weight and be boiled by fire, take out, cooling, filtering takes filtrate, be added 0.8 parts by weight urea stir to
Dissolving, obtains purslane extract.
As invention further embodiment：Step（1）The supersound process, power be 177W, frequency 125kHz
Under conditions of be ultrasonically treated 25min, pause 18min is ultrasonically treated under conditions of power is 115W, frequency is 87kHz
31min。
As invention further embodiment：Step（2）The cooling treatment, constant temperature under conditions of temperature is 1.4 DEG C
Cooling treatment 9min is warming up to 4.9 DEG C and continues constant temperature cooling treatment 19min.
As invention further embodiment：Step（3）The magnetization treatment, magnetic under conditions of magnetic field intensity is 680G
Change processing 220s, pause 60s, magnetization treatment 350s under conditions of magnetic field intensity is 1890G, pause 35 ~ 45s, strong in magnetic field
Degree is magnetization treatment 180s under conditions of 1180G.
Comparative example 1
The safflower oil tea cuttage processing method of the prior art
Comparative example 2
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in by step with embodiment 1
（1）The purslane extract is replaced with water, other conditions all same.
Comparative example 3
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in steps with embodiment 1（1）
Without being ultrasonically treated, other conditions all same.
Comparative example 4
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in steps with embodiment 1（2）
Without cooling treatment, other conditions all same.
Comparative example 5
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in steps with embodiment 1（3）
Without magnetization treatment, other conditions all same.
Comparative example 6
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in steps with embodiment 1（3）
Barium metaborate and citric acid chelated iron, other conditions all same are added without in the roots soaking agent.
Comparative example 7
A kind of cuttage processing method improving safflower oil tea cuttage radication capability, difference lies in steps with embodiment 1（3）
Curcumin and momordica grosvenori polysaccharide, other conditions all same are added without in the roots soaking agent.
Same a collection of safflower oil tea cuttage item is handled using the processing method of embodiment and comparative example, every group of processing
200, cuttage experiment is carried out after processing, in addition to the processing method of cuttage item is different, other conditions all same（Cuttage is tested complete
It is carried out under the conditions of light auto spraying；Cutting medium is turfy soil, and back cut diameter 6cm, depth 7cm, mesh hole are poured into after disinfection
In 50 plastic seeding culturing plate；Cuttage depth is the 1/2 of cutting length；The spray intervals time is adjusted according to Changes in weather after inserting, it is fine
It sprays 1 time every 5min when its high temperature, sprays 1 time every 10min when cloudy, spraying every time continues 30s）, right after cuttage 40 days
The cuttage root-taking situation of safflower oil tea cuttage item is detected, and detects its rooting rate, indefinite radical, average adventitious root length peace
Indefinite root long rugosity, as a result such as table 1：
The rootability comparing result of 1 embodiment and comparative example of table
| Rooting rate（%） | Indefinite radical（Item） | Average adventitious root length（cm） | Average indefinite root long rugosity（mm） | |
| Embodiment 1 | 98.5 | 15.4 | 8.91 | 3.39 |
| Embodiment 2 | 99 | 15.1 | 8.78 | 3.31 |
| Embodiment 3 | 98.5 | 14.9 | 8.58 | 3.36 |
| Comparative example 1 | 68.5 | 6.8 | 3.43 | 1.66 |
| Comparative example 2 | 89.5 | 12.1 | 6.33 | 2.78 |
| Comparative example 3 | 90.5 | 12.6 | 6.46 | 2.85 |
| Comparative example 4 | 93.0 | 13.5 | 7.18 | 3.01 |
| Comparative example 5 | 88.5 | 11.4 | 5.78 | 2.72 |
| Comparative example 6 | 88.5 | 11.8 | 5.97 | 2.69 |
| Comparative example 7 | 87.5 | 11.1 | 5.82 | 2.61 |
As it can be seen from table 1 the processing method of the safflower oil tea cuttage item of the present invention can dramatically increase safflower oil tea cuttage item
Rooting rate, indefinite radical, average adventitious root length and the long rugosity of average adventitious root, improve its rootability.
Claims (5)
1. a kind of cuttage processing method improving safflower oil tea cuttage radication capability, which is characterized in that the specific method is as follows：
（1）At the beginning of 7 months, select the lower part of safflower oil tea current-year branch as cuttage item, length is trimmed to 14 ~ 15cm, retains 2
Blade is immersed in purslane extract and impregnates 66 ~ 74min and be passed through ultrasonic wave and be ultrasonically treated, and filtrate is pulled out, and single treatment is obtained
Cuttage item；
（2）Constant temperature steam handles 6 ~ 7min under conditions of being 48 ~ 54 DEG C by single treatment cuttage item merging temperature, takes out, merging
Cooling treatment is carried out in cooler bin, obtains after-treatment cuttage item；
（3）After-treatment cuttage item is placed in magnetic field and carries out magnetization treatment, takes out, 3.4 ~ 3.8cm of lower end is immersed in leaching root
32 ~ 38min is impregnated in agent, filtering agent is pulled out, must wait for cuttage safflower oil tea cuttage item；
The roots soaking agent is made of the raw material of following parts by weight：0.82 ~ 0.88 part of barium metaborate, citric acid chelated iron 2.1 ~
2.5 parts, 0.23 ~ 0.27 part of curcumin, 0.31 ~ 0.35 part of momordica grosvenori polysaccharide, 170 ~ 190 parts of water.
2. the cuttage processing method according to claim 1 for improving safflower oil tea cuttage radication capability, which is characterized in that
Step（1）The purslane extract, is prepared according to the following steps：The purslane of 43 ~ 45 parts by weight is crushed, merging temperature is
Constant temperature steam handles 25 ~ 30min in 210 ~ 230 DEG C of steam box, takes out, and 18 ~ 20 parts by weight is added, mass fraction is 74% ~ 78%
Ethanol solution be mixed evenly, merging temperature is 80 ~ 90h of ferment at constant temperature in 38 ~ 40 DEG C of fermenting case, takes out, puts into
It is boiled by fire, takes out in the water of 120 ~ 130 parts by weight, cooling, filtering takes filtrate, and the urea stirring of 0.7 ~ 0.8 parts by weight is added
To dissolving, purslane extract is obtained.
3. the cuttage processing method according to claim 1 for improving safflower oil tea cuttage radication capability, which is characterized in that
Step（1）The supersound process, under conditions of power is 161 ~ 177W, frequency is 119 ~ 125kHz be ultrasonically treated 23 ~
25min, pause 14 ~ 18min, and 29 ~ 31min is ultrasonically treated under conditions of power is 103 ~ 115W, frequency is 81 ~ 87kHz.
4. the cuttage processing method according to claim 1 for improving safflower oil tea cuttage radication capability, which is characterized in that
Step（2）The cooling treatment, constant temperature 8 ~ 9min of cooling treatment under conditions of temperature is 0.8 ~ 1.4 DEG C, it is warming up to 4.3 ~
4.9 DEG C are continued 17 ~ 19min of constant temperature cooling treatment.
5. the cuttage processing method according to claim 1 for improving safflower oil tea cuttage radication capability, which is characterized in that
Step（3）The magnetization treatment, 200 ~ 220s of magnetization treatment under conditions of magnetic field intensity is 640 ~ 680G, pause 50 ~
60s, 330 ~ 350s of magnetization treatment under conditions of magnetic field intensity is 1830 ~ 1890G, pause 35 ~ 45s, is in magnetic field intensity
160 ~ 180s of magnetization treatment under conditions of 1140 ~ 1180G.
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| CN111903525A (en) * | 2020-08-17 | 2020-11-10 | 重庆福林农业生物技术研究院有限公司 | Induction method of embryogenic callus of camellia oleifera abel with large fruit |
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