CN104705083A - Method for promoting early blossoming of chrysanthemum by utilizing curcumin - Google Patents
Method for promoting early blossoming of chrysanthemum by utilizing curcumin Download PDFInfo
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- CN104705083A CN104705083A CN201510174074.1A CN201510174074A CN104705083A CN 104705083 A CN104705083 A CN 104705083A CN 201510174074 A CN201510174074 A CN 201510174074A CN 104705083 A CN104705083 A CN 104705083A
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- chrysanthemum
- curcumin
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- blooming
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Wood Science & Technology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a method for promoting precocious flowering of chrysanthemum by utilizing curcumin. The method comprises the following steps: by taking germination strips at the roots of robust chrysanthemum cutting branches as explants, performing tissue culture to establish a single-bud strain material, carrying out subculture on the single-bud strain material, adding curcumin with final concentration of 100-400mu mol/l into the subculture medium after performing subculture for 2-3 generations, and continuously treating the chrysanthemum buds for 30-40 days, performing conventional rooting and seedling exercising after treatment, and transplanting in farmland and performing normal culture management. The method is simple to operate, obvious in effect (the blossoming time of chrysanthemum is 5-8 days earlier than before), the appreciation value of the chrysanthemum cannot be influenced, and the method is applicable to large-scale production.
Description
Technical field
The present invention relates to the regulate and control method at a kind of plant florescence, be specifically related to a kind of method utilizing curcumin to promote chrysanthemum Blooming.
Background technology
Chrysanthemum has long cultivation history in China, because of features such as its pattern are various, flower pattern is different and the florescence is long, for having the flower variety of economic worth in modern flowers industry, has wide market and good prospect.At present, flouring due to large-scale activity festivals or holidays in various places, market need badly cultivate bloom during National Day in the early blossoming system of flower variety and late flowering variety.Therefore, the florescence, as one of chrysanthemum important economic worth, needs people to break through traditional control temperature and illumination method, seeks the control methods of effective, convenient practicality more economically.
Plant is called " flowering transition " from nourishing and growing to the conversion process of reproductive growth, and the key of this process is floral induction.Flowering Induction Process is determined by own genetic material and outside environmental elements two aspect.The regulatory pathway of external environment has a lot, mainly utilizes chrysanthemum flowers Bud polarization to the requirement of light application time and intensity in traditional chrysanthemum florescence control technique, by shading or light filling process, artificially manufactures short-day environment, impels chrysanthemum Blooming; In addition, people also can calculate sowing, cuttage, time of pruning, pinching according to cultivation experience, utilize pinching, prune, pluck the object that the measures such as flower bud, stripping bud priming leaf, girdling reach florescence control.These two kinds of methods all need veteran professional technician, and process is loaded down with trivial details, great loss human resources.
Chinese patent CN200810049322 discloses a kind of method of regulating florescence of chrysanthemum, and the method test-tube plantlet of 5-aza-cytidine process chrysanthemum tissue cultures, then transplants, make chrysanthemum Blooming.The medicine 5-azacitidine that the method uses, primarily of microbial metabolism generation or chemical method synthesis, does not have the biogenetic derivation of curcumin extensive.Curcumin can refine processing from multiple zingiberaceous plant (root tuber of aromatic turmeric, turmeric, curcuma zedoary etc.), cheap.
Summary of the invention
The object of the invention is to provide a kind of method utilizing curcumin to promote chrysanthemum Blooming, and the method is simple and easy to do, under the prerequisite not affecting chrysanthemum appreciation effect, makes chrysanthemum Blooming.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method utilizing curcumin to promote chrysanthemum Blooming, with the chrysanthemum cuttage seeding base portion rudiment bar of robust growth for explant, tissue cultures sets up simple bud based material, squamous subculture is carried out to simple bud based material, wherein, after squamous subculture 2-3 generation, in squamous subculture subculture medium used, add final concentration is that the curcumin of 100-400 μm of ol/l processes chrysanthemum young shoot 30-40 days continuously, through the taking root of routine, hardening after process, move into the normal cultivation management in field.
Concrete: described subculture medium consists of: MS+0.1mg/L NAA+1.0 mg/L 6-BA, pH 5.8.
Described cultivation management comprises to be extractd side shoot, lateral bud, superfluous bud in time and smears flower bud.
With the concrete grammar of methylation inhibitor curcumin process chrysanthemum young shoot be: curcumin is first used anhydrous alcohol solution, is mixed with suitable concentration; Then add in subculture medium, make its final concentration in subculture medium at 100-400 μm of ol/l.Processing time was advisable with 30-40 days.
In general, the 2-3 month processes sprout in containing the subculture medium of curcumin, and the 3-4 month carries out culture of rootage, (generally needing about one month) hardening when well developed root system stalwartness, move into land for growing field crops between late May to early June, liquid manure is sufficient to adopt conventional cultivation management method to ensure.
The inventive method is after adopting medicine curcumin process chrysanthemum material a period of time, by suppressing DNA methylation level, thus changes chrysanthemum epigenetic information, promotes that chrysanthemum florescence in advance.The inventive method in operation, by means of only adding medicine curcumin disposable process chrysanthemum material in subculture medium, reduces the input of manpower in operating process.Meanwhile, the inventive method utilizes vegetative propagation technique, and can not rearrange because of the epigenetic in gamete emergence period and these two stages of period of preimplantation and lose acquired epigenetic modification, the proterties of the advance flowering period obtained be comparatively stable.
Compared to the prior art, beneficial effect of the present invention:
On the quality base of flower guaranteeing chrysanthemum, achieve the object (florescence can shift to an earlier date about 5-8 days) promoting chrysanthemum Blooming, and can not affect the ornamental value of chrysanthemum, the early blossoming system that can cultivate improved seeds is to meet the demand in market.Meanwhile, the method simple operations, does not need the input of too much human and material resources, economical and effective, is applicable to lot-sizeization and produces.
Embodiment
Do to introduce in detail further to technical scheme of the present invention below in conjunction with embodiment, but protection scope of the present invention is not limited thereto.
Embodiment
The present embodiment tests " refreshing horse " Dendranthema morifolium Varieties, concrete control method is: early Febuary, with the chrysanthemum cuttage seeding base portion rudiment bar of robust growth for explant, tissue cultures (culture fluid consists of: MS+0.1mg/L NAA+2 mg/L 6-BA) sets up simple bud based material.Squamous subculture is carried out to simple bud based material, wherein, after squamous subculture 3 generation, (subculture medium consists of: MS+0.1mg/L NAA+1.0 mg/L 6-BA, pH 5.8), in squamous subculture subculture medium used, add final concentration is respectively that the curcumin of 100,200 and 400 μm of ol/l processes chrysanthemum young shoot 30 days continuously, through the taking root of routine, hardening after process, move into the normal cultivation management in field.Ensure in whole life cycle that liquid manure is sufficient, extract side shoot, lateral bud, superfluous bud in time, smear flower bud in time and ensure that a chrysanthemum opens a flower, be convenient to add up flowering time.
First day was counted the same day to land for growing field crops, flowering time in record process of the test (flowering time is told look for standard with bud and added up, and so-called bud tells look and bud has just shown colored color) from chrysanthemum transplantation of seedlings.Control group curcumin concentration counts 0 μm of ol/l, and result of the test is in table 1.
The results show: after the curcumin process for different Dendranthema morifolium Varieties concentration 100-400 μm ol/l, all can make chrysanthemum Blooming (in advance 5-8 days) to a certain extent, and the method is time saving and energy saving, simple to operation, compensate for the deficiency of light, warm regulation florescence.
It should be noted last that: above embodiment is only in order to explanation, and unrestricted technical scheme of the present invention, although with reference to above-described embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: still can modify to the present invention or equivalent replacement, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (3)
1. utilize curcumin to promote the method for chrysanthemum Blooming, with the chrysanthemum cuttage seeding base portion rudiment bar of robust growth for explant, tissue cultures sets up simple bud based material, squamous subculture is carried out to simple bud based material, it is characterized in that, after squamous subculture 2-3 generation, in squamous subculture subculture medium used, add final concentration is that the curcumin of 100-400 μm of ol/l processes chrysanthemum young shoot 30-40 days continuously, through the taking root of routine, hardening after process, move into the normal cultivation management in field.
2. utilize curcumin to promote the method for chrysanthemum Blooming as claimed in claim 1, it is characterized in that: described subculture medium consists of: MS+0.1mg/L NAA+1.0 mg/L 6-BA, pH 5.8.
3. utilize curcumin to promote the method for chrysanthemum Blooming as claimed in claim 1, it is characterized in that: described cultivation management comprises to be extractd side shoot, lateral bud, superfluous bud in time and smears flower bud.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510174074.1A CN104705083A (en) | 2015-04-14 | 2015-04-14 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510174074.1A CN104705083A (en) | 2015-04-14 | 2015-04-14 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
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| CN104705083A true CN104705083A (en) | 2015-06-17 |
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| CN201510174074.1A Pending CN104705083A (en) | 2015-04-14 | 2015-04-14 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN111034482A (en) * | 2020-01-15 | 2020-04-21 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN114711076A (en) * | 2022-03-23 | 2022-07-08 | 昆明学院 | High-yield and high-quality cultivation method for promoting differentiation of long-spike mulberry buds |
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| JP2006321721A (en) * | 2005-05-17 | 2006-11-30 | Shinto Paint Co Ltd | Blooming and fruit-bearing promoter |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN111034482A (en) * | 2020-01-15 | 2020-04-21 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN114711076A (en) * | 2022-03-23 | 2022-07-08 | 昆明学院 | High-yield and high-quality cultivation method for promoting differentiation of long-spike mulberry buds |
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Application publication date: 20150617 |
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