CN102487698B - Method for promoting chrysanthemum to bloom in advance by using 5-azaC - Google Patents
Method for promoting chrysanthemum to bloom in advance by using 5-azaC Download PDFInfo
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- CN102487698B CN102487698B CN 201110413963 CN201110413963A CN102487698B CN 102487698 B CN102487698 B CN 102487698B CN 201110413963 CN201110413963 CN 201110413963 CN 201110413963 A CN201110413963 A CN 201110413963A CN 102487698 B CN102487698 B CN 102487698B
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Abstract
The invention relates to a method for promoting chrysanthemum to bloom in advance by using 5-azaC. The method comprises the following steps of: cutting and raising a seedling; after rooting, planting in a cultivation pot, and adopting the conventional cultivation management method to ensure water and fertilizer; and when a chrysanthemum plant has the height of 20-40cm, treating the apical meristem of the chrysanthemum by using the 5-azaC at the concentration of 200-500mu mol/l for 10 to 15 days, and restoring normal management. The method is easy to implement, has an obvious effect (the chrysanthemum can bloom 10 to15 days earlier), cannot generate any adverse effect on the enjoyment effect of the chrysanthemum, and is suitable for scale production in field.
Description
Technical field
The present invention relates to the regulate and control method at a kind of plant florescence, be specifically related to the method that a kind of 5-azaC of utilization promotes the chrysanthemum Blooming.
Background technology
Chrysanthemum is divided into spring chrysanthemum (late April at florescence is to late May) according to the nature florescence; Xia Ju (late May at florescence is to August); Qiu Ju (late October at florescence is to late November); Winter chrysanthemum (February of early December at florescence to next year).Chrysanthemum is wide in variety because of it, the flower type is good, nice selection becomes the most widely kind of cultivation.Chrysanthemum grow, germinate the differentiation and bloom, with environmental factors such as sunshine, temperature and cultivations extremely close relation is arranged.Chrysanthemum is carried out the regulation and control such as artificial sunshine, temperature, chrysanthemum is done sth. in advance or the delay florescence according to people's wish.Florescence is called " forcing culture " ahead of time than the nature florescence, and the florescence then is called " suppressing cultivation " than nature flowering delay.
The complete life cycle of plant is divided into nourishes and grows and two stages of reproductive growth, wherein is called " becoming flower to change " by nourishing and growing to the conversion process of reproductive growth, and the key of this process is floral induction.The floral induction process of higher plant is by self genetic material and the decision of external environment factor two aspects.Chrysanthemum is the short-day flowers, finishes under the long-day condition and nourishes and grows, and carries out reproductive growth under the short-day condition.In order to satisfy people's demand to chrysanthemum when the non-natural florescence, can be the physiological property of short-day plant according to chrysanthemum, artificial reforming Dendranthema Growth environment or transformation chrysanthemum hereditary information, impel chrysanthemum to accelerate to grow, turn to reproductive growth from nourishing and growing, cause flower bud differentiation, thereby reach the purpose of Blooming.
Light application time is envirment factor important in the Dendranthema Growth growth course; flower bud differentiation plays an important role to chrysanthemum; tradition chrysanthemum florescence control technique is exactly to utilize the chrysanthemum flower bud differentiation to the requirement of light application time and intensity, processes that by shading or light filling chrysanthemum was bloomed at the improper florescence.This technology is strict to illumination condition, must carry out continuously, and interruption or interval are processed and all can be caused short-day to be processed unsuccessfully.In addition, this operating process is loaded down with trivial details, and the short-day processing time is long, and time-consuming, effort is not easy to the land for growing field crops large-scale production.Chinese patent CN200810049322 discloses a kind of method of regulating florescence of chrysanthemum, and the method is processed the test-tube plantlet that the chrysanthemum tissue is cultivated with 5-aza-cytidine (5-azaC), then transplants, and makes the chrysanthemum Blooming.The method is cultivated because relating to tissue, and is the processing time long (needing 30 days), also more loaded down with trivial details.
Summary of the invention
The object of the invention is to provide a kind of 5-azaC of utilization to promote the method for chrysanthemum Blooming, and the method is simple and easy to do, under the prerequisite that does not affect the chrysanthemum appreciation effect, makes the chrysanthemum Blooming.
For achieving the above object, the present invention adopts following technical scheme:
A kind of method of utilizing 5-azaC to promote the chrysanthemum Blooming, comprise cuttage and seedling culture, be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height is 20～40cm, use methylation inhibitor 5-aza-cytidine (5-azaC) to process the apical meristem of chrysanthemum, recover normal management after processing.
The described apical meristem of processing chrysanthemum with methylation inhibitor 5-azaC is specially: drawing 400～500 μ l concentration with cotton balls is that the 5-azaC(water of 200～500 μ mol/l is mixed with respective concentration) be placed on chrysanthemum plant terminal bud (also can adopt the mode that sprays to carry out medication), once a day, processed 10-15 days.
Preferably, cultivation in the alms bowl used cultivation matrix can for: volume ratio is refuse soil, the peat composed of rotten mosses and the chicken manure of 3:1:1; Conventional cultivation management comprises timely excision side shoot, lateral bud, superfluous bud and smears flower bud.
Further, the described method of utilizing 5-azaC to promote the chrysanthemum Blooming, in the early June cuttage and seedling culture, be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height is 25～35cm (this moment is generally to early August), drawing 500 μ l concentration with cotton balls is that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud, once a day, keep recovering normal management after 10 days.
The inventive method is to adopt medicine 5-azaC to process the chrysanthemum apical meristem after a period of time, by changing the chrysanthemum epigenetic information, promote chrysanthemum to enter in advance reproductive growth after nourishing and growing finishing, can remedy like this defective of light, temperature control technology, regulating florescence of chrysanthemum makes its Blooming more easily.Adopt the inventive method can exempt the loaded down with trivial details work that the flower-grower carries out shading or light filling every day, and the method is simple and easy to operate in implementation process, does not need the professional and technical personnel to implement.Process change chrysanthemum epigenetic information by 5-azaC and realize Blooming, treatment effect is more stable, has also reduced short-day and has processed the risk that causes, and is more suitable for the large tracts of land production application.
The inventive method solved the operating process of traditional chrysanthemum florescence control technique loaded down with trivial details, have a big risk, time-consuming, the effort problem, and realized equally promoting the purpose (chrysanthemum can shift to an earlier date 10-15 days and bloom) of Blooming, can be for giving security with flower during " 11 " National Day.The method is simple to operate, and successful can not produce any negative effect to the chrysanthemum appreciation effect, is applicable to the land for growing field crops large-scale production.
Embodiment
Below in conjunction with embodiment technical scheme of the present invention is done to introduce in detail further, but protection scope of the present invention is not limited to this.
Embodiment 1
Present embodiment is that " hydrangea " Dendranthema morifolium Varieties is tested, and concrete control method is: early June cuttage and seedling culture, (cultivation matrix in the cultivation alms bowl consists of V to be colonizated in the cultivation alms bowl after taking root
Refuse soil: V
The peat composed of rotten mosses: V
Chicken manure=3:1:1), cultivation alms bowl bore 19cm, high 15cm.Guarantee that liquid manure is sufficient in vegetative period, in time extract side shoot, lateral bud, superfluous bud, in time smear flower bud and guarantee that a chrysanthemum opens a flower, be convenient to add up flowering time (be colonizated in the cultivation alms bowl from the chrysanthemum seedling and counted first day the same day).August 15 is when being 30cm when chrysanthemum seedling plant height, experimental group is drawn the 5-azaC aqueous solution that 500 μ l concentration are respectively 200,250,300,400 and 500 μ mol/l with cotton balls and is placed on chrysanthemum plant terminal bud, every day, processing once (was changed treatment fluid every afternoon 1 time), recover normal management after continuous 10 days, flowering time in the record process of the test (tell look take bud and add up as standard, and it is that bud has just shown colored color that so-called bud is told look by flowering time; Together lower).Control group is smeared distilled water (5-azaC concentration is counted 0 μ mol/l), and result of the test sees Table 1.
Embodiment 2
Present embodiment is that " the spring township is soaring " Dendranthema morifolium Varieties is tested, and concrete control method is: early June cuttage and seedling culture, (cultivation matrix in the cultivation alms bowl consists of V to be colonizated in the cultivation alms bowl after taking root
Refuse soil: V
The peat composed of rotten mosses: V
Chicken manure=3:1:1), cultivation alms bowl bore 19cm, high 15cm.Guarantee that liquid manure is sufficient in vegetative period, in time extract side shoot, lateral bud, superfluous bud, in time smear flower bud and guarantee that a chrysanthemum opens a flower, be convenient to add up flowering time (be colonizated in the cultivation alms bowl from the chrysanthemum seedling and counted first day the same day).August 15 is when being 30cm when chrysanthemum seedling plant height, draw the 5-azaC aqueous solution that 500 μ l concentration are respectively 200,250,300,400 and 500 μ mol/l with cotton balls and be placed on chrysanthemum plant terminal bud, every day, processing once (was changed treatment fluid every afternoon 1 time), recovered normal management after continuous 10 days.Control group is smeared distilled water (5-azaC concentration is counted 0 μ mol/l), and result of the test sees Table 2.
The results show: smear after the top processes with the 5-azaC of concentration 200～500uM for different Dendranthema morifolium Varieties, all can make to a certain extent chrysanthemum Blooming (10～15 days in advance), and the method is time saving and energy saving, simple to operation, has remedied the deficiency of light, warm regulation florescence.
It should be noted last that: above embodiment is only in order to explanation, and unrestricted technical scheme of the present invention, although with reference to above-described embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1. method of utilizing 5-azaC to promote the chrysanthemum Blooming, comprise cuttage and seedling culture, be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient, it is characterized in that, when the chrysanthemum plant height was 20～40cm, working concentration was the apical meristem that the methylation inhibitor 5-azaC of 200～500 μ mol/l processes chrysanthemum, recovered normal management after processing.
2. utilize as claimed in claim 1 5-azaC to promote the method for chrysanthemum Blooming, it is characterized in that: the described apical meristem of processing chrysanthemum with methylation inhibitor 5-azaC is specially: drawing 400～500 μ l concentration with cotton balls is that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud, once a day, processed 10-15 days.
3. utilize as claimed in claim 1 5-azaC to promote the method for chrysanthemum Blooming, it is characterized in that: used cultivation matrix is in the cultivation alms bowl: volume ratio is refuse soil, the peat composed of rotten mosses and the chicken manure of 3:1:1; Conventional cultivation management comprises timely excision side shoot, lateral bud, superfluous bud and smears flower bud.
4. utilize as claimed in claim 1 5-azaC to promote the method for chrysanthemum Blooming, it is characterized in that, in the early June cuttage and seedling culture, be colonizated in after taking root in the cultivation alms bowl, adopt conventional cultivation management method to guarantee that liquid manure is sufficient, when the chrysanthemum plant height was 25～35cm, drawing 500 μ l concentration with cotton balls was that the 5-azaC of 200～500 μ mol/l is placed on chrysanthemum plant terminal bud, once a day, keep recovering normal management after 10 days.
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| CN 201110413963 CN102487698B (en) | 2011-12-13 | 2011-12-13 | Method for promoting chrysanthemum to bloom in advance by using 5-azaC |
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| CN 201110413963 CN102487698B (en) | 2011-12-13 | 2011-12-13 | Method for promoting chrysanthemum to bloom in advance by using 5-azaC |
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| CN102487698B true CN102487698B (en) | 2013-02-06 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104705083A (en) * | 2015-04-14 | 2015-06-17 | 河南大学 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
| CN105580806B (en) * | 2016-02-15 | 2018-09-28 | 河南师范大学 | A kind of method of chrysanthemum florescence composite regulator and regulating florescence of chrysanthemum |
| CN107950540B (en) * | 2017-11-22 | 2020-05-26 | 青岛农业大学 | Application of 5-azacytidine in preparation of bactericide for preventing and treating plant diseases caused by phytopathogens |
| CN108901580A (en) * | 2018-07-25 | 2018-11-30 | 河南科技大学 | A method of promoting eary maturity of grape |
| CN110042120A (en) * | 2019-05-07 | 2019-07-23 | 沈阳农业大学 | The method for improving soybean resistance inducing clumping bud efficiency using 5- aza-cytidine |
| CN111034482B (en) * | 2020-01-15 | 2021-10-01 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN115735772B (en) * | 2022-12-01 | 2023-08-29 | 青岛农业大学 | Application of 5-AzaC in apple stock tissue rooting culture or sugarless rooting culture |
| CN117204220B (en) * | 2023-09-13 | 2024-06-11 | 扬州大学 | Treatment method for improving seed production rate of cucumber seed melon |
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| JPH01235527A (en) * | 1988-03-14 | 1989-09-20 | Akita Pref Gov | Dwarfing of normal plant using 5-azacitidine |
| CN101347075B (en) * | 2008-03-07 | 2011-04-20 | 河南大学 | Method for regulating florescence of chrysanthemum |
| CN101755582A (en) * | 2009-09-29 | 2010-06-30 | 孔赟荣 | Chrysanthemum out-of-season blooming cultivation technique and method |
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