CN103250579B - The technology of a seasonal termination of diapause of fruit tree - Google Patents

The technology of a seasonal termination of diapause of fruit tree Download PDF

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CN103250579B
CN103250579B CN201310162772.0A CN201310162772A CN103250579B CN 103250579 B CN103250579 B CN 103250579B CN 201310162772 A CN201310162772 A CN 201310162772A CN 103250579 B CN103250579 B CN 103250579B
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fruit tree
fruit
seasonal
dormancy
planting
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CN103250579A (en
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高志红
庄维兵
章镇
王真
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Nanjing Agricultural Univ
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Nanjing Agricultural Univ
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Abstract

The present invention relates to the method for the seasonal termination of diapause of a planting fruit-trees.The processing method of the seasonal termination of diapause of one planting fruit-trees, comprising: (1) chilling requirement meets the fruit tree containing full bud that three/collection is for the moment in hibernation, and uses GA 4solution carries out water planting process; (2) fruit tree of water planting process is put in illumination box, within 10 days, adds up the germination rate of fruit tree afterwards, determine its resting state.Compared with chemicals for breaking dormancy-calcium cyanamide conventional at present, GA 4toxicity is little, prepares its solution and sprays also simple than smearing calcium cyanamide, GA 4more effective than calcium cyanamide to the broken dormancy effect of fruit tree bud; Adopt GA 4dormancy process is carried out brokenly to fruit tree there are good theoretical foundation and market prospects.

Description

The technology of a seasonal termination of diapause of fruit tree
Technical field
The present invention relates to the method for the seasonal termination of diapause of a planting fruit-trees, be exclusively used in the seasonal dormancy removing fruit tree.
Background technology
Chilling requirement is the demand to resting stage low temperature amount that plant is formed in long-term nature evolution process, only meet chilling requirement, guarantee its smoothly by natural dormancy, if chilling requirement is not enough, plant can not normally complete natural dormancy overall process, dysplasia can be caused, even if condition is suitable for, can not sprouts optimum period, or sprout irregular, and cause floral organ deformity or serious abortion, affect the yield and quality of fruit.Some areas of south China, " warm winter " problem becomes increasingly conspicuous, and the chilling requirement of fruit tree is not being met, and adopts chemicals for breaking dormancy can Substitute For Partial chilling requirement, makes trees breaking dormancy comparatively early, normal vital movement after starting.Chemicals for breaking dormancy conventional at present comprises the nitrogen-containing compounds such as calcium cyanamide, polyamines, salicylic acid and potassium nitrate, and also nobody adopts GA 4process of sleeping is carried out brokenly to fruit tree.
Summary of the invention
The present invention be directed to some areas of south China, " warm winter " problem becomes increasingly conspicuous, and the chilling requirement of fruit tree is not being met, and affects the yield and quality of fruit; Simultaneously along with the lifting of living standard, people increase greatly for the demand of anti-season fruit, and fruit tree bud has become the important means improving economy of the fruit tree benefit ahead of time.And adopt chemicals for breaking dormancy can Substitute For Partial chilling requirement, make trees breaking dormancy comparatively early, normal vital movement beginning after, do sth. in advance the time that protected fruit trees cultivation button canopy is heated, to gather ahead of time, produce economic benefit more better than common facility cultivation.
The object of the invention is to be achieved through the following technical solutions:
The processing method of the seasonal termination of diapause of one planting fruit-trees, comprising:
(1) chilling requirement meets the fruit tree containing full bud that three/collection is for the moment in hibernation, and uses GA 4solution carries out water planting process;
(2) fruit tree of water planting process is put in illumination box cultivates, within 10 days, add up the germination rate of fruit tree afterwards, determine its resting state.
The fruit tree of the 20-30cm containing full bud that described fruit tree is preferably in hibernation.
Preferred employing 50 μMs of-400 μMs of GA 4water planting process is carried out to fruit tree, most preferably adopts 200 μMs of GA 4water planting process is carried out to fruit tree.
Described condition of culture is: h/ in daytime 12 h at night 12, and temperature is 25 ± 1.0 DEG C/night 18 ± 1.0 DEG C in daytime, intensity of illumination 55 μm of olm 2s 1, relative air humidity 70%; Within every 2 days, change 1 culture fluid, wipe out base portion 2 mm at every turn when changing water, expose new stubble.
The standard of described germination rate: with bud top cracking, reveal green being as the criterion.
Cultivate and add up bud germination rate afterwards in 10 days, as germination rate is more than or equal to 50%, be then in breaking dormancy state, otherwise be then in resting state.
Compared to the prior art, beneficial effect of the present invention:
Compared with chemicals for breaking dormancy-calcium cyanamide conventional at present, GA 4toxicity is little, prepares its solution and sprays also simple than smearing calcium cyanamide, GA 4more effective than calcium cyanamide to the broken dormancy effect of fruit tree bud; Adopt GA 4dormancy process is carried out brokenly to fruit tree there are good theoretical foundation and market prospects.
Embodiment
1, GA is determined 4debita spissitudo
Gather the two planting fruit-trees cherries ' red light ' of the 20-30cm containing full bud be in hibernation and the branch of fruit plum ' after rich ', adopt 50 μMs of GA 4, 100 μMs of GA 4, 200 μMs of GA 4, 300 μMs of GA 4, 400 μMs of GA 4carry out water planting process; The fruit tree of water planting process is put in illumination box, within 10 days, adds up the germination rate of fruit tree afterwards; Condition of culture is: h/ in daytime 12 h at night 12, and temperature is 25 ± 1.0 DEG C/night 18 ± 1.0 DEG C in daytime, intensity of illumination 55 μm of olm 2s 1, relative air humidity 70%; Within every 2 days, change 1 culture fluid, wipe out base portion 2 mm at every turn when changing water, expose new stubble.Study the effect that it removes seasonal dormancy, result shows 200 μMs of GA 4the effect of breaking dormancy is best, and too high or too low effect is all not as 200 μMs of GA 4remarkable (see table 1 and table 2).
Table 1 different times adopts the GA of variable concentrations 4the broken dormancy effect of process to cherry ' red light '
Table 2 different times adopts the GA of variable concentrations 4process the broken dormancy effect to fruit plum ' after rich '
2, determine to adopt GA 4remove the toeatment period of the seasonal dormancy of fruit tree
Much research adopts 0-7.2 DEG C of model to draw, and fruit plum ' after rich ' and the chilling requirement of cherry ' red light ' are respectively 1148h and 1200h, and we are respectively 410h and 370h according to the low temperature cumulant on the December 31st, 2012 of the data acquisition 0-7.2 DEG C modeling statistics on moisture recorder and on December 17th, 2012, account for 1/3rd of its chilling requirement.Gather weekly two planting fruit-trees branch samples, with 200 μMs of GA 4carry out brokenly dormancy process to it, research shows to use 200 μMs of GA when fruit tree chilling requirement meets for three/a period of time 4most pronounced effects, all can not remove the seasonal dormancy of fruit tree too early or excessively evening preferably.
3, GA provided by the invention 4remove the processing method of the seasonal dormancy of fruit tree
Gather weekly the two planting fruit-trees branches of 20-30cm containing full bud be in hibernation, every planting fruit-trees branch is divided into two groups: one group with 200 μMs of GA 4carry out water planting process to it, another group with water treatment as a control group; Two groups of fruit trees are put in illumination box and cultivate, within 10 days, add up the germination rate of fruit tree afterwards, determine its resting state.
Condition of culture is: h/ in daytime 12 h at night 12, and temperature is 25 ± 1.0 DEG C/night 18 ± 1.0 DEG C in daytime, intensity of illumination 55 μm of olm 2s 1, relative air humidity 70%; Within every 2 days, change 1 culture fluid, wipe out base portion 2 mm at every turn when changing water, expose new stubble.Result is in table 1 and table 2.
(1) the water treatment contrast growing state of fruit tree
Result shows, cherry ' red light ' fruit tree of the 20-30cm containing full bud be in hibernation of collection on January 7th, 2013, and put into water, in illumination box after 10 days, germination rate reaches more than 50%, namely relieves seasonal dormancy; Fruit plum ' after the rich ' fruit tree of the 20-30cm containing full bud be in hibernation of collection on February 1st, 2013, put into water, in illumination box after 10 days, germination rate reaches more than 50%, namely relieves seasonal dormancy.
(2) 200 μMs of GA are adopted 4carry out the releasing process of the seasonal dormancy of fruit tree
Result shows, sample cherry ' red light ' fruit tree of the 20-30cm containing full bud be in hibernation of collection on December 17th, 2011, puts into 200 μMs of GA 4in, in illumination box after 10 days, germination rate reaches more than 50%, namely relieves seasonal dormancy; Fruit plum ' after the rich ' fruit tree of the 20-30cm containing full bud be in hibernation of collection on December 31st, 2012, puts into 200 μMs of GA 4in, in illumination box after 10 days, germination rate reaches more than 50%, namely relieves seasonal dormancy.
Cherry ' red light ' is compared with control group, and seasonal termination of diapause has done sth. in advance 21 days, and fruit plum ' after rich ' compared with control group, has done sth. in advance 32 days, Be very effective (table 3).
Table 32 planting fruit-trees 200 μMs of GA 4the date statistics of process and water treatment breaking dormancy

Claims (2)

1. the processing method of the seasonal termination of diapause of a planting fruit-trees, is characterized in that comprising:
(1) chilling requirement meets the fruit tree containing full bud that three/collection is for the moment in hibernation, and adopts 200 μMs of GA 4solution carries out water planting process to fruit tree;
(2) be put in illumination box by the fruit tree of water planting process and cultivate, condition of culture is: 12h/ in daytime 12h at night, and temperature is 25 ± 1.0 DEG C/night 18 ± 1.0 DEG C in daytime, intensity of illumination 55 μm of olm -2s -1, relative air humidity 70%; Within every 2 days, change 1 culture fluid, when changing water, wipe out base portion 2mm at every turn, expose new stubble; Within 10 days, add up the germination rate of fruit tree afterwards, determine its resting state.
2. the processing method of the seasonal termination of diapause of fruit tree according to claim 1, is characterized in that described fruit tree is the fruit tree of the 20-30cm containing full bud be in hibernation.
CN201310162772.0A 2013-05-03 2013-05-03 The technology of a seasonal termination of diapause of fruit tree Active CN103250579B (en)

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Publication number Priority date Publication date Assignee Title
CN103636684B (en) * 2013-12-05 2015-06-10 成都新朝阳作物科学有限公司 Fruit tree dormancy breaking agent and application thereof
CN107345247B (en) * 2017-06-23 2020-09-22 北京林业大学 Molecular detection method for plum blossom bud dormant state
CN107494008A (en) * 2017-09-26 2017-12-22 界首市朝鹏家庭农场 A kind of method for releasing pear tree bud dormancy

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CN101120634A (en) * 2007-09-11 2008-02-13 南京林业大学 Method for rapid relieving dormant state of nanjing linden seed
CN101861822A (en) * 2010-06-07 2010-10-20 北京林业大学 Peony cut-flower forcing culture method
CN102598984A (en) * 2012-03-22 2012-07-25 中国农业科学院蔬菜花卉研究所 Cultivation method for promoting pot-cultured tree peony to blossom on National Day

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101120634A (en) * 2007-09-11 2008-02-13 南京林业大学 Method for rapid relieving dormant state of nanjing linden seed
CN101861822A (en) * 2010-06-07 2010-10-20 北京林业大学 Peony cut-flower forcing culture method
CN102598984A (en) * 2012-03-22 2012-07-25 中国农业科学院蔬菜花卉研究所 Cultivation method for promoting pot-cultured tree peony to blossom on National Day

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