CN101347075B - Method for regulating florescence of chrysanthemum - Google Patents
Method for regulating florescence of chrysanthemum Download PDFInfo
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- CN101347075B CN101347075B CN200810049322XA CN200810049322A CN101347075B CN 101347075 B CN101347075 B CN 101347075B CN 200810049322X A CN200810049322X A CN 200810049322XA CN 200810049322 A CN200810049322 A CN 200810049322A CN 101347075 B CN101347075 B CN 101347075B
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Abstract
The invention discloses a method for regulating and controlling chrysanthemum flowering season. More particularly, the method comprises the following steps: tender stem apexes are taken from field chrysanthemum plants as an explant; tissue culture is carried out, then a single bud material is established, that is, a certain single bud is reproduced in successive generations; the single bud material is treated by methylation inhibitor for 30 days, the treated material undergoes rooting culture and is finally transplanted to the field for cultivation management. The method of the invention adopts 5-azacytidine chemical treatment to change the epigenetic information of chrysanthemum in tissue culture, therefore, the chrysanthemum blooms 7-9 days in advance, and the effect can be passed on by vegetative propagation, and in the next year, the plants growing from the treated material roots can still bloom 7-9 days in advance.
Description
Technical field
The present invention relates to a kind of plant florescence control method, relate in particular to a kind of method of regulating florescence of chrysanthemum.
Background technology
Chrysanthemum is one of fresh cut-flowers famous on the present International Flower market, also is to cultivate the most popular pot flowers.Chrysanthemum is at the cultivation history in existing more than 3,000 year of China.According to incompletely statistics, the nearly kind more than 7000 of present global chrysanthemum kind.Chrysanthemum is divided into spring chrysanthemum (late April at florescence is to late May) according to the nature florescence; Xia Ju (late May at florescence is to August); Qiu Ju (late October at florescence is to late November); Cold chrysanthemum (February of 12 the first tenday period of a month to next year at florescence).In general, growth and development of plant, flower bud differentiation and bloom have extremely close relation with environmental factors such as sunshine, temperature and cultivations.Chrysanthemum is no exception, and the intensity of the counterglow that grows of chrysanthemum and length are very responsive, but because of the difference of kind and kind, conditions such as needed sunshine, temperature are also different, and flowering stage is also just different.
Qiu Ju is the extended familys in the chrysanthemum, and it is wide in variety, good, the nice selection of flower type, is to cultivate chrysanthemum kind the most widely.To the autumn chrysanthemum carry out regulation and control such as artificial sunshine, temperature, can make Qiu Ju ahead of time or postpone the florescence by people's wish.Florescence is called forcing culture ahead of time than the nature florescence, and the florescence then is called than nature flowering delay and suppresses cultivation.The forcing culture of Qiu Ju is the characteristic according to the flower bud differentiation needs short-day condition of chrysanthemum, by to the autumn chrysanthemum carry out short-day and handle and to make its early flowering.Its method be with light-proof materials such as black plastic film, shading screen in the morning with the natural lighting that hides at dusk chrysanthemum last autumn, the light application time of every day was controlled at 9～10 hours.During doing short-day and handling, shading continuously can not be interrupted, and a bit printing opacity must not be arranged, the hole on the veil and night street lamp, auto lamp etc. all can influence the short-day treatment effect.When doing the short-day processing, guarantee in light application time, to have sufficient illumination and suitable water and fertilizer management again simultaneously.Different kinds can be different to the length in short-day processing time.Generally speaking, autumn 15～20 ℃ by day of the chrysanthemums of 25～30 centimetres of plant heights under night about 10 ℃ the condition, can be finished flower bud differentiation in 10～15 days; 45～55 days, after enriching and be painted, bud can remove veil.Existing chrysanthemum florescence control technique adopts light, temperature control technology, adjusts chrysanthemum normal physiological process of growth: in certain vegetative reproduction time, carry out shading or light filling every day and handle, do sth. in advance or the postponement flowering time.But the enforcement of this kind technology requires strict to illumination, and street lamp and auto lamp all can have influence on treatment effect, and the management of aspects such as soil, water, fertilizer and damage by disease and insect also will strengthen, otherwise is difficult to reach the effect of exceeding the time limit.Adopt this kind method to waste time and energy.
Summary of the invention
The object of the present invention is to provide a kind of method of regulating florescence of chrysanthemum,, and prolonged the florescence of chrysanthemum so that chrysanthemum blooms in advance.
To achieve these goals, technical scheme of the present invention has adopted a kind of method of regulating florescence of chrysanthemum, specifically may further comprise the steps: get young tender stem end as explant from field chrysanthemum plant, carry out tissue culture, set up the simple bud based material then, promptly breed by generation with certain single bud; The methylation inhibitor that the simple bud based material carried out 30 days is handled, handled material and carry out culture of rootage, change the field cultivation management at last again over to.
The medium of described tissue culture is: MS+6-BA 2mg/L+NAA 0.1mg/L; Culturing room's condition is: temperature 24-25 ℃, and illumination 12-13hd
-1, illuminance is 1500Lx.
Described methylation inhibitor is 5-aza-cytidine (5-azaC) or is 5-azadeoxycytidine (5-aza-dC).
The 5-azaC concentration of treatment that the simple bud based material is handled is 10uM and 1uM.
The culture fluid of described culture of rootage is 1/2MS+NAA 0.1mg/L.
Method of the present invention is to adopt medicine with after the chrysanthemum seedling processing certain hour, carries out the land for growing field crops again and cultivates, and can remedy the defective of light, temperature control technology like this, and regulating florescence of chrysanthemum is bloomed it in advance more easily.Adopt method of the present invention can exempt the loaded down with trivial details work that the flower-grower carries out shading or light filling every day, it is not very strict that the land for growing field crops is required, and plant can the lasting for years early flowering.Method of the present invention adds methylation inhibitor in medium, stem apex is carried out 30 days processing after, carry out culture of rootage and change grown in field then over to, guarantee that the required liquid manure of plant normal growth gets final product.Method of the present invention is passed through tissue culture, adopt pentaaza cytidine drug treating, change the epigenetic information of chrysanthemum, thereby making chrysanthemum shift to an earlier date 7-9 days blooms, and this effect may be transmitted by nourishing and generating, and the plant that grows on the material root of handling still can shift to an earlier date 7-9 days in 1 year and bloom.At present, obtaining success by test.Method of the present invention operation is simple relatively, the cost of throwing lower, time saving and energy saving, the method for regulating florescence of chrysanthemum more originally is easy, has broad application prospects.
Embodiment
Embodiment 1
Present embodiment is that " Huang on National Day " chrysanthemum kind is tested, concrete control method is: get young tender stem end as explant from field chrysanthemum plant, carry out tissue culture, culture fluid is MS+6-BA 2mg/L+NAA 0.1mg/L, the temperature 24-25 of culturing room ℃, illumination 12h/d, illuminance 1500Lx; Set up simple bud based material (breeding by generation) with certain single bud; The 5-aza-cytidine that the simple bud based material carried out 30 days is handled, and concentration of treatment is 10uM, 1uM; Handle material and carry out culture of rootage, culture fluid is: 1/2MS+NAA 0.1mg/L; Change the field cultivation management at last again over to.
Embodiment 2
Present embodiment is that " black kylin " chrysanthemum kind is tested, concrete control method is: get young tender stem end as explant from field chrysanthemum plant, carry out tissue culture, culture fluid is MS+6-BA 2mg/L+NAA 0.1mg/L, the temperature 24-25 of culturing room ℃, illumination 13h/d, illuminance 1500Lx; Set up simple bud based material (breeding by generation) with certain single bud; The 5-aza-cytidine that the simple bud based material carried out 30 days is handled, and concentration of treatment is 10uM, 1uM; Handle material and carry out culture of rootage, culture fluid is: 1/2MS+NAA 0.1mg/L; Change the field cultivation management at last again over to.
5-azaC handles the material flowering time relatively
| Different disposal concentration | Observation plant number | Flowering time (my god) |
| 10uM | 15 | 182±0.941 |
| 1uM | 15 | 180.5±0.256 |
| 0uM (contrast) | 15 | 189±0.527 |
Experimental results show that: only guaranteeing under the low level management condition of moisture, its result proves that 5-azaC (5-aza-cytidine) handles and still can do sth. in advance the florescence to a certain extent, these are handled material continue cultivation, in the newborn plant in 1 year, still have identical effect.Thereby the new technology that proves this regulation florescence can be done sth. in advance the florescence really, and time saving and energy saving, has remedied the deficiency of light, warm regulation florescence.
It should be noted last that: above embodiment is only in order to explanation, and unrestricted technical scheme of the present invention, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (5)
1. the method for a regulating florescence of chrysanthemum is characterized in that: specifically may further comprise the steps: get young tender stem end as explant from field chrysanthemum plant, carry out tissue culture, set up the simple bud based material then, promptly breed by generation with certain single bud; The methylation inhibitor that the simple bud based material carried out 30 days is handled, handled material and carry out culture of rootage, change the field cultivation management at last again over to.
2. the method for regulating florescence of chrysanthemum according to claim 1, it is characterized in that: the medium of described tissue culture is: MS+6-BA 2mg/L+NAA 0.1mg/L; Culturing room's condition is: temperature 24-25 ℃, and illumination 12-13hd
-1, illuminance is 1 500Lx.
3. the method for regulating florescence of chrysanthemum according to claim 1 is characterized in that: described methylation inhibitor is the 5-aza-cytidine or is the 5-azadeoxycytidine.
4. the method for regulating florescence of chrysanthemum according to claim 1 is characterized in that: the 5-azaC concentration of treatment that the simple bud based material is handled is 10uM or 1uM.
5. the method for regulating florescence of chrysanthemum according to claim 1, it is characterized in that: the culture fluid of described culture of rootage is 1/2MS+NAA 0.1mg/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810049322XA CN101347075B (en) | 2008-03-07 | 2008-03-07 | Method for regulating florescence of chrysanthemum |
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| CN200810049322XA CN101347075B (en) | 2008-03-07 | 2008-03-07 | Method for regulating florescence of chrysanthemum |
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| CN101347075A CN101347075A (en) | 2009-01-21 |
| CN101347075B true CN101347075B (en) | 2011-04-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901580A (en) * | 2018-07-25 | 2018-11-30 | 河南科技大学 | A method of promoting eary maturity of grape |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487698B (en) * | 2011-12-13 | 2013-02-06 | 河南大学 | Method for promoting chrysanthemum to bloom in advance by using 5-azaC |
| CN103250529B (en) * | 2013-04-17 | 2014-12-17 | 池州市天健生态农业发展有限公司 | Method of cultivating chrysanthemum by probiotic nutrients |
| CN103609351B (en) * | 2013-12-10 | 2015-11-25 | 河南师范大学 | A kind of method of regulating florescence of chrysanthemum |
| CN104705083A (en) * | 2015-04-14 | 2015-06-17 | 河南大学 | Method for promoting early blossoming of chrysanthemum by utilizing curcumin |
| CN106636193B (en) * | 2017-01-23 | 2020-11-03 | 河南大学 | Method for regulating chrysanthemum flowering time by using RNAi (ribonucleic acid interference) vector |
| CN111034482B (en) * | 2020-01-15 | 2021-10-01 | 河南大学 | Method for treating chrysanthemum cutting to promote chrysanthemum to bloom in advance |
| CN113853978A (en) * | 2021-11-01 | 2021-12-31 | 河南科技大学 | Application of methylthioadenosine in promoting grape fruit precocity, grape precocity promoter and method |
| CN115735772B (en) * | 2022-12-01 | 2023-08-29 | 青岛农业大学 | Application of 5-AzaC in apple stock tissue rooting culture or sugarless rooting culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108901580A (en) * | 2018-07-25 | 2018-11-30 | 河南科技大学 | A method of promoting eary maturity of grape |
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| CN101347075A (en) | 2009-01-21 |
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