CN106376328A - Wild bletila striata seed quick breeding method - Google Patents

Wild bletila striata seed quick breeding method Download PDF

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Publication number
CN106376328A
CN106376328A CN201610764723.8A CN201610764723A CN106376328A CN 106376328 A CN106376328 A CN 106376328A CN 201610764723 A CN201610764723 A CN 201610764723A CN 106376328 A CN106376328 A CN 106376328A
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China
Prior art keywords
seed
bletilla striata
wild
bletillae
rhizoma bletillae
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CN201610764723.8A
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Chinese (zh)
Inventor
陈蕾
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YUNNAN JINBI PHARMACEUTICAL CO Ltd
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YUNNAN JINBI PHARMACEUTICAL CO Ltd
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Priority to CN201610764723.8A priority Critical patent/CN106376328A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

Abstract

A wild bletila striata seed quick breeding method relates to a plant culturing and breeding method, and in particular relates to a medicine culturing and breeding method. In the method, matured seeds are cultured; and germination rate can be greatly improved by 100% when the seeds are cultured via culture medium, so quick seed source expansion can be achieved. The wild bletila striata seed quick breeding method is simple and adaptive, requires low cost and improves seed germination rate; seedlings cultured by the method are strong; seeds can be fully germinated in one month with proper culture medium, so bletila striata culturing and breeding time can be shortened; and large-scale plantation for wilde bletila striata domestication become possible.

Description

Wild bletilla striata seed method for quickly breeding
Technical field
The present invention relates to a kind of culturing and reproducing of plant, particularly to the culturing and reproducing of medicine.
Background technology
Pseudobulbus Bletillae (Rhizoma Bletillae) Bletilla striata (Thunb.) Reichb.f. belongs to herbaceos perennial for the orchid family Pseudobulbus Bletillae (Rhizoma Bletillae), has Astringing to arrest bleeding, detumescence and promoting granulation, treatment traumatic hemorrhage, sore swollen toxin, chapped skin and pulmonary tuberculosis hemoptysis, ulcer haemorrhage etc. are medicinal It is worth, also there is beauty treatment, whitening, speckle dispelling and ornamental value simultaneously and be widely used, its wild resource is endangered.
Bletilla striata seeds are tiny, like powder, do not have endosperm, the embryo of ateliosis, are difficult to germinate, are difficult to carry out seed numerous Grow;During using division propagation, low reproduction rate, reproduction speed are slow.
Content of the invention
The purpose of the present invention is intended to overcome the defect of prior art, provides one kind to be bred by tissue cultivating, and Breeding potential is high, fireballing wild bletilla striata seed method for quickly breeding.
Wild bletilla striata seed method for quickly breeding of the present invention, its operating procedure is:
A. seed selects:First-selected perennial, abundant ripe wild bletilla striata seed, embryo age and bletilla striata seeds germination rate are just in Correlation, the bletilla striata seeds germination rate in big embryo age is 100%;
B. take Pseudobulbus Bletillae (Rhizoma Bletillae) maturation capsule, 2~3 fruits are placed in and bind up with gauze, tap water rinses 1~2 hour, enter ultra-clean Chemical industry station;
C. use aseptic water washing 1 time in superpurgative working table through the good Pseudobulbus Bletillae (Rhizoma Bletillae) fruit of step b water process, mass concentration is 75% ethanol disinfection 25-40 second, aseptic water washing 1 time;0.1% mercuric chloride soaks 10-11 minute, aseptic water washing 3-4 time;
D. the Pseudobulbus Bletillae (Rhizoma Bletillae) mature fruit tip after step c aseptic process is cut, the seed in fruit is uniformly dispensed in sprouting Send out media surface;
E. breeding condition controls in 25 DEG C of temperature, light intensity 1500~25001x, daily illumination 9~10 hours;Day and night temperature 18-25 DEG C, promote seed germination and growth under luminous environment or completely black dark breeding condition;
F., after seed germination, cultivate 35-45 days under illumination condition, Pseudobulbus Bletillae (Rhizoma Bletillae) height of seedling degree about 1.5-2.0cm simultaneously has blade exhibition Transplant when opening to the perfect field of drainage system;
G. field is combined using booth and sunshade net, and every mu of the Pseudobulbus Bletillae (Rhizoma Bletillae) cultivates 6,000 12000 plants/acre;
H. the field management of Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling, weeding moisturizing, spray a potassium dihydrogen phosphate or thin people and animals' fecaluria every month, Spray 6-9 month, that is, enter rest period.
Culture medium described in step d, is with MS as minimal medium, and adds the AC of NAA, 6.0g/L of 1.0mg/L.
Culture medium described in step d, is with MS as minimal medium, and adds the 6- of NAA, 1.0mg/L of 1.0mg/L BA.
Culture medium described in step d, is with 1/2MS as minimal medium, and add NAA, 6.0g/L of 1.0mg/L AC.
Culture medium described in step d, is with 1/2MS as minimal medium, and add NAA, 1.0mg/L of 1.0mg/L 6-BA.
After weeding moisturizing described in step h is seed plantation, sprays Acetochlor closing and kill weeds, after Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling all sprouts Using sunshade, it is to avoid direct sunlight, moisturizing of simultaneously watering, shallow hoe during weeding, it is to avoid hinder root.
Moisturizing described in step h is sooner or later respectively to pour a water in 7,8,9 three months.
Wild bletilla striata seed method for quickly breeding of the present invention, using ripe cultivating seeds, through culture medium culturing Afterwards, its germination rate is greatly improved, and can reach 100%, to adapt to rapid expansion introduces a collection.Cultivation side of the present invention Method is simple, applicable, low cost, not only increases the germination rate of seed, and the Seedling cultivated is more thick and strong, in suitable culture In the case of base provisions, a substantially all rudiment in month, substantially reduce the ectogenesis time of the Pseudobulbus Bletillae (Rhizoma Bletillae), be the instruction of wild bletilla striata Support the possibility providing implantation in large scale.
Specific embodiment
With reference to embodiment, the present invention is described further, but is not limited to embodiment.
Embodiment 1
Described wild bletilla striata seed method for quickly breeding, its operating procedure is:
A. seed selects:First-selected perennial, abundant ripe wild bletilla striata seed, embryo age and bletilla striata seeds germination rate are just in Correlation, the bletilla striata seeds germination rate in big embryo age is 100%, and the bletilla striata seeds germination rate in low embryo age is relatively low;
B. take Pseudobulbus Bletillae (Rhizoma Bletillae) maturation capsule, 2~3 fruits are placed in and bind up with gauze, tap water rinses 1~2 hour, enter ultra-clean Chemical industry station;
C. use aseptic water washing 1 time in superpurgative working table through the good Pseudobulbus Bletillae (Rhizoma Bletillae) fruit of step b water process, mass concentration is 75% ethanol disinfection 25-40 second, aseptic water washing 1 time;0.1% mercuric chloride soaks 10-11 minute, aseptic water washing 3-4 time;
D. the Pseudobulbus Bletillae (Rhizoma Bletillae) mature fruit tip after step c aseptic process is cut, the seed in fruit is uniformly dispensed in sprouting Send out media surface, described culture medium, be with MS as minimal medium, and add the AC of NAA, 6.0g/L of 1.0mg/L, Under conditions of this culture medium, the germination rate highest of bletilla striata seeds, reach 100%, and seedling is sturdy;
E. breeding condition controls in 25 DEG C of temperature, light intensity 1500~25001x, daily illumination 9~10 hours;Day and night temperature 18-25 DEG C, promote seed germination and growth under luminous environment or completely black dark breeding condition;
F., after seed germination, cultivate 35-45 days under illumination condition, Pseudobulbus Bletillae (Rhizoma Bletillae) height of seedling degree about 1.5-2.0cm simultaneously has blade exhibition Transplant when opening to the perfect field of drainage system;
G. field is combined using booth and sunshade net, and every mu of the Pseudobulbus Bletillae (Rhizoma Bletillae) cultivates 6,000 12000 plants/acre;
H. the field management of Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling, weeding moisturizing, described weeding, after being seed plantation, spray Acetochlor closing and kill Go out weeds, the Pseudobulbus Bletillae (Rhizoma Bletillae) requires strict in field management weeding, will prevent weed growth, will accomplish timely weeding, during weeding will shallow hoe, So as not to hindering root;Described moisturizing is to adopt sunshade net after Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling all sprouts, it is to avoid direct sunlight, moisturizing of simultaneously watering, weeding When shallow hoe, it is to avoid hinder root, described moisturizing is sooner or later respectively to pour a water, because the Pseudobulbus Bletillae (Rhizoma Bletillae) is liked cloudy, often keep moistening, will when arid Water, 7~September part respectively pours a water sooner or later.The Pseudobulbus Bletillae (Rhizoma Bletillae) is afraid of flood again, and the timely draining of heavy rain avoids hindering root;The Pseudobulbus Bletillae (Rhizoma Bletillae) is the fertile plant of happiness, Spray a potassium dihydrogen phosphate or thin people and animals' fecaluria every month, after October, taper off growth, enter dormancy.Plantation 2 Year, nearly 2 tons of yield.
Embodiment 2
Described wild bletilla striata seed method for quickly breeding, the culture medium described in step d, is with MS as minimal medium, And add the 6-BA of NAA, 1.0mg/L of 1.0mg/L, under conditions of this culture medium, the germination rate of bletilla striata seeds is higher, reaches 98.6%, and seedling is sturdy;Remaining is with embodiment 1.
Embodiment 3
Described wild bletilla striata seed method for quickly breeding, the culture medium described in step d, is to be cultivated with 1/2MS for basic Base, and add the AC of NAA, 6.0g/L of 1.0mg/L, under conditions of this culture medium, the germination rate of bletilla striata seeds is higher, reaches 99%, and seedling is sturdy;Remaining is with embodiment 1.
Embodiment 4
Described wild bletilla striata seed method for quickly breeding, the culture medium described in step d, is to be cultivated with 1/2MS for basic Base, and add the 6-BA of NAA, 1.0mg/L of 1.0mg/L, under conditions of this culture medium, the germination rate highest of bletilla striata seeds, Reach 97%, and seedling is sturdy;Remaining is with embodiment 1.

Claims (7)

1. a kind of wild bletilla striata seed method for quickly breeding is it is characterised in that its operating procedure is:
A. seed selects:First-selected perennial, abundant ripe wild bletilla striata seed, embryo age and bletilla striata seeds germination rate are in positive Close, the bletilla striata seeds germination rate in big embryo age is 100%;
B. take Pseudobulbus Bletillae (Rhizoma Bletillae) maturation capsule, 2~3 fruits are placed in and bind up with gauze, tap water rinses 1~2 hour, enter ultra-clean chemical industry Station;
C. use aseptic water washing 1 time in superpurgative working table through the good Pseudobulbus Bletillae (Rhizoma Bletillae) fruit of step b water process, mass concentration is 75% The ethanol disinfection 25-40 second, aseptic water washing 1 time;0.1% mercuric chloride soaks 10-11 minute, aseptic water washing 3-4 time;
D. the Pseudobulbus Bletillae (Rhizoma Bletillae) mature fruit tip after step c aseptic process is cut, the seed in fruit is uniformly dispensed in sprouting training Foster primary surface;
E. breeding condition controls in 25 DEG C of temperature, light intensity 1500~25001x, daily illumination 9~10 hours;Day and night temperature 18-25 DEG C, promote seed germination and growth under luminous environment or completely black dark breeding condition;
F. after seed germination, cultivate 35-45 days under illumination condition, Pseudobulbus Bletillae (Rhizoma Bletillae) height of seedling degree about 1.5-2.0cm when having mounted blade Transplant to the perfect field of drainage system;
G. field is combined using booth and sunshade net, and every mu of the Pseudobulbus Bletillae (Rhizoma Bletillae) cultivates 6,000 12000 plants/acre;
H. the field management of Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling, weeding moisturizing, spray a potassium dihydrogen phosphate or thin people and animals' fecaluria every month, spray 6-9 month, that is, enter rest period.
2. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that culture described in step d Base, is with MS as minimal medium, and adds the AC of NAA, 6.0g/L of 1.0mg/L.
3. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that culture described in step d Base, is with MS as minimal medium, and adds the 6-BA of NAA, 1.0mg/L of 1.0mg/L.
4. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that culture described in step d Base, is with 1/2MS as minimal medium, and adds the AC of NAA, 6.0g/L of 1.0mg/L.
5. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that culture described in step d Base, is with 1/2MS as minimal medium, and adds the 6-BA of NAA, 1.0mg/L of 1.0mg/L.
6. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that weeding described in step h After moisturizing is seed plantation, sprays Acetochlor closing and kill weeds, adopt sunshade after Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling all sprouts, it is to avoid direct sunlight, Water moisturizing simultaneously, shallow hoe during weeding, it is to avoid hinder root.
7. wild bletilla striata seed method for quickly breeding according to claim 1 is it is characterised in that moisturizing described in step h It is sooner or later respectively to pour a water in 7,8,9 three months.
CN201610764723.8A 2016-08-30 2016-08-30 Wild bletila striata seed quick breeding method Pending CN106376328A (en)

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Cited By (6)

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Publication number Priority date Publication date Assignee Title
CN106922536A (en) * 2017-04-13 2017-07-07 毕节市农业科学研究所 A kind of method that fast energy-saving cultivates bletilla seedling
CN107046851A (en) * 2017-04-12 2017-08-18 西南林业大学 Bletilla greenhouse direct sowing and seedling method by material of seed
CN107173222A (en) * 2017-03-27 2017-09-19 河池乐康生态农业科技有限公司 A kind of wild bletilla striata quick breeding method for tissue culture
CN107306790A (en) * 2017-06-30 2017-11-03 福建省农业科学院农业生物资源研究所 The tissue culture and rapid propagation method of bletilla microtubers
CN109566312A (en) * 2019-01-10 2019-04-05 张家界长见农业科技有限公司 A kind of method of bletilla striata seeds direct sowing and seedling
CN109699422A (en) * 2019-03-14 2019-05-03 四川康养健生物科技有限公司 A kind of mating system of fritillaria cirrhosa seeds

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CN104335903A (en) * 2014-11-21 2015-02-11 广西中医药大学 Method for accelerating quick propagation of rhizoma bletillae
CN104429865A (en) * 2014-12-23 2015-03-25 湖北济世药业有限责任公司 Sexual propagation method of bletilla striata

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107173222A (en) * 2017-03-27 2017-09-19 河池乐康生态农业科技有限公司 A kind of wild bletilla striata quick breeding method for tissue culture
CN107046851A (en) * 2017-04-12 2017-08-18 西南林业大学 Bletilla greenhouse direct sowing and seedling method by material of seed
CN107046851B (en) * 2017-04-12 2020-07-10 西南林业大学 Bletilla striata greenhouse direct seeding seedling method taking seeds as materials
CN106922536A (en) * 2017-04-13 2017-07-07 毕节市农业科学研究所 A kind of method that fast energy-saving cultivates bletilla seedling
CN107306790A (en) * 2017-06-30 2017-11-03 福建省农业科学院农业生物资源研究所 The tissue culture and rapid propagation method of bletilla microtubers
CN107306790B (en) * 2017-06-30 2019-05-31 福建省农业科学院农业生物资源研究所 The tissue culture and rapid propagation method of bletilla microtubers
CN109566312A (en) * 2019-01-10 2019-04-05 张家界长见农业科技有限公司 A kind of method of bletilla striata seeds direct sowing and seedling
CN109699422A (en) * 2019-03-14 2019-05-03 四川康养健生物科技有限公司 A kind of mating system of fritillaria cirrhosa seeds

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Application publication date: 20170208