CN105532471A - Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method - Google Patents

Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method Download PDF

Info

Publication number
CN105532471A
CN105532471A CN201610019921.1A CN201610019921A CN105532471A CN 105532471 A CN105532471 A CN 105532471A CN 201610019921 A CN201610019921 A CN 201610019921A CN 105532471 A CN105532471 A CN 105532471A
Authority
CN
China
Prior art keywords
days
seedling
sealwort
culture
illumination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610019921.1A
Other languages
Chinese (zh)
Inventor
鞠建文
游永铃
苏志杭
叶祖云
刘群
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujian Zhe Canzhong Industry Co Ltd
Original Assignee
Fujian Zhe Canzhong Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Zhe Canzhong Industry Co Ltd filed Critical Fujian Zhe Canzhong Industry Co Ltd
Priority to CN201610019921.1A priority Critical patent/CN105532471A/en
Publication of CN105532471A publication Critical patent/CN105532471A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Abstract

The invention discloses a novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method and relates to the technical field of traditional Chinese medicine cultivation. The novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method comprises the six steps of explant obtaining and processing, aseptic seedling induction, tuber propagation, tuber adventitious-bud induction and strong seedling rooting, polygonatum cyrtonema seedling acclimatization and polygonatum cyrtonema seedling transplantation in fields. The novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method establishes an intermediate polygonatum cyrtonema propagation system, and a large amount of detoxified polygonatum cyrtonema seedlings can be quickly obtained; the survival rate of transplanted tuber seedlings is high, and the seedlings grow fast in a later period; the emerged polygonatum cyrtonema seedlings grow in order, do not easily fall ill and are excellent in quality, management is simplified, the cost is low, and the cultivation method has popularization value.

Description

A kind of large-scale planting new method of David's-harp high yield and high quality
Technical field
The present invention relates to Chinese medicine cultivation technical field, particularly a kind of large-scale planting new method of David's-harp high yield and high quality.
Background technology
David's-harp system Liliaceae Polygonatum herbaceos perennial, integrates medicinal, eats, views and admires and its cosmetic values.Sealwort kind is a lot, and what use as Chinese medicine has P. kingianum, sealwort, David's-harp, wherein with David's-harp performance optimal.David's-harp rhizome contains abundant carbohydrate, steroid saponin, cardiac glycoside, alkaloid, flavones and anthraquinone analog compound, lignanoid, vitamin, amino acid and micro-number of chemical composition, it is China's tradition bulk medicinal materials, there is the effects such as boosting qi and nourishing yin, invigorating the spleen, moistening lung, kidney-nourishing, inhibition tumor cell, there is very important value of exploiting and utilizing, in development new drug and exploitation health products, also there are bright prospects.Along with people grow with each passing day to the demand of sealwort, wild resource far can not meet need of production, and standardization artificial cultivation is imperative.
David's-harp modes of reproduction mainly contains seminal propagation, stem tuber breeds two kinds of modes.The factors such as David's-harp seed maturity is late, germination rate is low, seedling raise period is long have impact on sealwort large-scale production cultivation greatly; Stem tuber breeding is current tame common method, but because every sealwort only has green tape sprout tuber stem then to breed, reproduction coefficient is low, and rhizome consumption is large, both uneconomical, limit scale again, simultaneously because of the accumulation of long-term vegetative propagation stem tuber virus, seedling is degenerated, and is unfavorable for that cultivation scale specification is promoted, therefore, a kind of production method that can solve David's-harp factory culture is sought extremely urgent.
Summary of the invention
The invention provides a kind of large-scale planting new method of David's-harp high yield and high quality; overcoming the breeding of current David's-harp stem tuber, to there is reproduction coefficient low; rhizome consumption is large, both uneconomical, limit scale again; and vegetative propagation stem tuber virus accumulation throughout the year; seedling is degenerated, and is unfavorable for the defect that cultivation scale specification is promoted therefore the invention provides a kind of product of fine quality, with low cost; can a large amount of virus-elimination seedlings of quick obtaining, there is the large-scale planting new method of the David's-harp high yield and high quality of promotional value.
The present invention is achieved through the following technical solutions: a kind of large-scale planting new method of David's-harp high yield and high quality, comprises the steps:
(1) explant obtains and process: get the sprouting that David's-harp stem tuber is not sprouted, and peels off under layer 2-3 outsourcing sheet is placed on running water, through flowing water cleaning 2h, be put in sterilizing small beaker and be transferred on superclean bench under laboratory;
(2) aseptic seedling induction: be 70% ethanol sterilization treatment 30s by the explant concentration that step (1) is handled well, sterile water wash 2 times, putting into concentration is again that 0.1%-0.2% mercuric chloride is sterilized 8min, sterile water wash 5 times, peel off the crystal shaped stem apex cell mass of 0.2-0.5mm size with transfer needle under stereoscopic border, stem apex is transferred and to cultivate in MS+4.0mg/LBA+0.4mg/LNAA medium, the sucrose of medium supplemented 3%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, 7-15 days early stage cultivated until sealwort bud grows under complete darkness condition, turn intensity of illumination 2000Lx, illumination 12h, under dark 12h, Cyclic culture obtains aseptic detoxic seedling in 40 days afterwards, detoxic seedling inductivity reaches 30%,
(3) propagation of stem tuber: the aseptic seedling that step (2) obtains is forwarded in MS+6.0mg/LBA+0.2mg/LNAA medium and cultivates, the sucrose of described medium supplemented 3%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, within first 7 days, cultivates under half-light, turning intensity of illumination after 7 days is 2000Lx, within illumination 12h, dark 12h Cyclic culture 40-50 days, obtains propagation stem tuber; Greatly reduce Multiple Buds brownization in cultivation dead, improve the rate of increase;
(4) stem tuber adventitious bud inducing and shape seedling rooting: the increment stem tuber that step (3) obtains is transferred and to cultivate in MS+0.1mg/LNAA medium, the sucrose of described medium supplemented 4%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, and intensity of illumination is 2000Lx, within illumination 12h, dark 12h Cyclic culture 30-40 days, can obtain band bud, be with root to be good for the sealwort seedling of shape; The plantation of band sprout tuber stem can substantially increase transplanting survival rate;
(5) sealwort seedling domestication: the sealwort seedling that step (4) obtains is moved in the booth of ventilation March, cultivate 10 days in natural lighting situation, open after bottle cap cultivates 3 days, take out seedling cleaning and remove medium, transplant to on the seedbed of detritus soil or turfy soil 2 parts+vermiculite 1 part, overlay film cultivates 30 days, opens two film, open film after 7 days completely to cultivate, within first 7 days, carry out foliar spray management according to the growth of cereal crop seedlings;
(6) sealwort seedling field-transplanting: the sealwort will tamed through step (5) is transplanted to land for growing field crops, can obtain sealwort stem tuber after 1 year, then gather in the crops sealwort finished product through the plantation of 2 years.
Compared with prior art, the present invention has following outstanding advantages and effect: (1) the present invention establishes the rapid propagation system of detoxification sealwort, can obtain a large amount of detoxification sealwort seedling fast; (2) stem tuber seedling transplanting survival rate is high, and late growing stage is fast; (3) sealwort emerges growth neatly, and not easily fall ill, streamlining management, product are of fine quality, with low cost, have promotional value.
Embodiment
Following Examples further illustrates the present invention, but should as restriction of the present invention:
Embodiment 1: a kind of large-scale planting new method of David's-harp high yield and high quality, comprises the steps:
(1) explant obtains and process: get the sprouting that David's-harp stem tuber is not sprouted, and peels off under layer 2-3 outsourcing sheet is placed on running water, through flowing water cleaning 2h, be put in sterilizing small beaker and be transferred on superclean bench under laboratory;
(2) aseptic seedling induction: be 70% ethanol sterilization treatment 30s by the explant concentration that step (1) is handled well, sterile water wash 2 times, putting into concentration is again that 0.1% mercuric chloride is sterilized 8min, sterile water wash 5 times, the crystal shaped stem apex cell mass of 0.5mm size is got under stereoscopic border, stem apex is transferred and to cultivate in MS+4.0mg/LBA+0.4mg/LNAA medium, the sucrose of medium supplemented 3%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, 7 days early stages cultivated until sealwort bud grows under complete darkness condition, turn intensity of illumination 2000Lx, illumination 12h, under dark 12h, Cyclic culture obtained aseptic seedling after 40 days, detoxic seedling inductivity reaches 30%,
(3) propagation of stem tuber: the aseptic seedling that step (2) obtains is forwarded in MS+6.0mg/LBA+0.2mg/LNAA medium and cultivates, the sucrose of described medium supplemented 3%, 0.5% agar, pH is 5.8, controls condition of culture, described condition of culture is: temperature 25 DEG C, within first 7 days, cultivate under half-light, turning intensity of illumination after 7 days is 2000Lx, and under illumination 12h, dark 12h, Cyclic culture obtains propagation stem tuber for 50 days, greatly reduce Multiple Buds brownization in cultivation dead, improve the rate of increase;
(4) stem tuber adventitious bud inducing and shape seedling rooting: the increment stem tuber that step (3) obtains is transferred and to cultivate in MS+0.1mg/LNAA medium, the sucrose of described medium supplemented 4%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, and intensity of illumination is 2000Lx, the sealwort seedling that under illumination 12h, dark 12h, Cyclic culture can obtain band bud for 30 days, band root is good for shape, the plantation of band sprout tuber stem can substantially increase transplanting survival rate;
(5) sealwort seedling domestication: the sealwort seedling that step (4) obtains is moved in the booth of ventilation March, cultivate 10 days in natural lighting situation, open after bottle cap cultivates 3 days, take out seedling cleaning and remove medium, transplant extremely with on the seedbed of detritus soil 2 parts+vermiculite 1 part, overlay film cultivates 30 days, opens two film, open film after 7 days completely to cultivate, within first 7 days, carry out foliar spray management according to the growth of cereal crop seedlings;
(6) sealwort seedling field-transplanting: the sealwort will tamed through step (5) is transplanted to land for growing field crops, can obtain sealwort stem tuber after 1 year, then gather in the crops sealwort finished product through the plantation of 2 years.
Embodiment 2: a kind of large-scale planting new method of David's-harp high yield and high quality, comprises the steps:
(1) explant obtains and process: get the sprouting that David's-harp stem tuber is not sprouted, and peels off under layer 2-3 outsourcing sheet is placed on running water, through flowing water cleaning 2h, be put in sterilizing small beaker and be transferred on superclean bench under laboratory;
(2) aseptic seedling induction: be 70% ethanol sterilization treatment 30s by the explant concentration that step (1) is handled well, sterile water wash 2 times, putting into concentration is again that 0.2% mercuric chloride is sterilized 8min, sterile water wash 5 times, the crystal shaped stem apex cell mass of 0.5mm size is got under stereoscopic border, stem apex is transferred and to cultivate in MS+4.0mg/LBA+0.4mg/LNAA medium, the sucrose of medium supplemented 3%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, 10 days early stages cultivated until sealwort bud grows under complete darkness condition, turn intensity of illumination 2000Lx, illumination 12h, under dark 12h, Cyclic culture obtained aseptic seedling after 40 days, detoxic seedling inductivity reaches 30%,
(3) propagation of stem tuber: the aseptic seedling that step (2) obtains is forwarded in MS+6.0mg/LBA+0.2mg/LNAA medium and cultivates, the sucrose of described medium supplemented 3%, 0.5% agar, pH is 5.8, controls condition of culture, described condition of culture is: temperature 25 DEG C, within first 7 days, cultivate under half-light, turning intensity of illumination after 7 days is 2000Lx, and under illumination 12h, dark 12h, Cyclic culture obtains propagation stem tuber for 40 days, greatly reduce Multiple Buds brownization in cultivation dead, improve the rate of increase;
(4) stem tuber adventitious bud inducing and shape seedling rooting: the increment stem tuber that step (3) obtains is transferred and to cultivate in MS+0.1mg/LNAA medium, the sucrose of described medium supplemented 4%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, and intensity of illumination is 2000Lx, the sealwort seedling that under illumination 12h, dark 12h, Cyclic culture can obtain band bud for 40 days, band root is good for shape, the plantation of band sprout tuber stem can substantially increase transplanting survival rate;
(5) sealwort seedling domestication: the sealwort seedling that step (4) obtains is moved in the booth of ventilation March, cultivate 10 days in natural lighting situation, open after bottle cap cultivates 3 days, take out seedling cleaning and remove medium, transplant extremely with on the seedbed of turfy soil 2 parts+vermiculite 1 part, overlay film cultivates 30 days, opens two film, open film after 7 days completely to cultivate, within first 7 days, carry out foliar spray management according to the growth of cereal crop seedlings;
(6) sealwort seedling field-transplanting: the sealwort will tamed through step (5) is transplanted to land for growing field crops, can obtain sealwort stem tuber after 1 year, then gather in the crops sealwort finished product through the plantation of 2 years.
Embodiment 3: a kind of large-scale planting new method of David's-harp high yield and high quality, comprises the steps:
(1) explant obtains and process: get the sprouting that David's-harp stem tuber is not sprouted, and peels off under layer 2-3 outsourcing sheet is placed on running water, through flowing water cleaning 2h, be put in sterilizing small beaker and be transferred on superclean bench under laboratory;
(2) aseptic seedling induction: be 70% ethanol sterilization treatment 30s by the explant concentration that step (1) is handled well, sterile water wash 2 times, putting into concentration is again that 0.15% mercuric chloride is sterilized 8min, sterile water wash 5 times, get under stereoscopic border 0.5mm size crystal shaped stem apex cell mass, stem apex is transferred and to cultivate in MS+4.0mg/LBA+0.4mg/LNAA medium, the sucrose of medium supplemented 3%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, 15 days early stages cultivated until sealwort bud grows under complete darkness condition, turn intensity of illumination 2000Lx, illumination 12h, under dark 12h, Cyclic culture obtained aseptic seedling after 40 days, detoxic seedling inductivity reaches 30%,
(3) propagation of stem tuber: the aseptic seedling that step (2) obtains is forwarded in MS+6.0mg/LBA+0.2mg/LNAA medium and cultivates, the sucrose of described medium supplemented 3%, 0.5% agar, pH is 5.8, controls condition of culture, described condition of culture is: temperature 25 DEG C, within first 7 days, cultivate under half-light, turning intensity of illumination after 7 days is 2000Lx, and under illumination 12h, dark 12h, Cyclic culture obtains propagation stem tuber for 45 days, greatly reduce Multiple Buds brownization in cultivation dead, improve the rate of increase;
(4) stem tuber adventitious bud inducing and shape seedling rooting: the increment stem tuber that step (3) obtains is transferred and to cultivate in MS+0.1mg/LNAA medium, the sucrose of described medium supplemented 4%, 0.5% agar, pH is 5.8, control condition of culture, described condition of culture is: temperature 25 DEG C, and intensity of illumination is 2000Lx, the sealwort seedling that under illumination 12h, dark 12h, Cyclic culture obtains band bud for 35 days, band root is good for shape, the plantation of band sprout tuber stem can substantially increase transplanting survival rate;
(5) sealwort seedling domestication: the sealwort seedling that step (4) obtains is moved in the booth of ventilation March, cultivate 10 days in natural lighting situation, open after bottle cap cultivates 3 days, take out seedling cleaning and remove medium, transplant extremely with on the seedbed of detritus soil 2 parts+vermiculite 1 part, overlay film cultivates 30 days, opens two film, open film after 7 days completely to cultivate, within first 7 days, carry out foliar spray management according to the growth of cereal crop seedlings;
(6) sealwort seedling field-transplanting: the sealwort will tamed through step (5) is transplanted to land for growing field crops, can obtain sealwort stem tuber after 1 year, then gather in the crops sealwort finished product through the plantation of 2 years.
Embodiment 4: the Fast-propagation effect of each embodiment
Increment multiple Rooting rate Transplanting survival rate
Embodiment 1 11.8 96% 95%
Embodiment 2 12.3 94% 97%
Embodiment 3 12.6 93% 96%
The above; be only the specific embodiment of the present invention; but protection scope of the present invention is not limited thereto; any those of ordinary skill in the art are in the technical scope disclosed by the present invention; the change can expected without creative work or replacement, all should be encompassed within protection scope of the present invention.

Claims (5)

1. a large-scale planting new method for David's-harp high yield and high quality, is characterized in that comprising the steps:
(1) explant obtains and process: get the sprouting that David's-harp stem tuber is not sprouted, and peels off under layer 2-3 outsourcing sheet is placed on running water, through flowing water cleaning 2h, be put in sterilizing small beaker and be transferred on superclean bench under laboratory;
(2) aseptic seedling induction: the explant that step (1) is handled well is carried out sterilization treatment, peel off the crystal shaped stem apex cell mass of 0.2-0.5mm size with transfer needle under stereoscopic border, stem apex is transferred and to cultivate in MS+4.0mg/LBA+0.4mg/LNAA medium, the sucrose of medium supplemented 3%, 0.5% agar, pH is 5.8, controls condition of culture;
(3) propagation of stem tuber: the aseptic seedling that step (2) obtains be forwarded in MS+6.0mg/LBA+0.2mg/LNAA medium and cultivate, the sucrose of described medium supplemented 3%, 0.5% agar, pH is 5.8, controls condition of culture;
(4) stem tuber adventitious bud inducing and shape seedling rooting: transferred by the increment stem tuber that step (3) obtains and to cultivate in MS+0.1mg/LNAA medium, the sucrose of described medium supplemented 4%, 0.5% agar, pH is 5.8, controls condition of culture;
(5) sealwort seedling domestication: the sealwort seedling that step (4) obtains is moved in the booth of ventilation March, cultivate 10 days in natural lighting situation, open after bottle cap cultivates 3 days, take out seedling cleaning and remove medium, transplant to on the seedbed of detritus soil or turfy soil 2 parts+vermiculite 1 part, overlay film cultivates 30 days, opens two film, open film after 7 days completely to cultivate, within first 7 days, carry out foliar spray management according to the growth of cereal crop seedlings;
(6) sealwort seedling field-transplanting: the sealwort will tamed through step (5) is transplanted to land for growing field crops, can obtain sealwort stem tuber after 1 year, then gather in the crops sealwort finished product through the plantation of 2 years.
2. the large-scale planting new method of a kind of David's-harp high yield and high quality according to claim 1; it is characterized in that step (2) described sterilization treatment is for being 70% ethanol sterilization treatment 30s by concentration; sterile water wash 2 times; putting into concentration is again that 0.1%-0.2% mercuric chloride is sterilized 8min, sterile water wash 5 times.
3. the large-scale planting new method of a kind of David's-harp high yield and high quality according to claim 1; it is characterized in that step (2) described condition of culture is: temperature 25 DEG C; 7-15 days early stage cultivated until sealwort bud grows under complete darkness condition; turn intensity of illumination 2000Lx, under illumination 12h, dark 12h, Cyclic culture obtains aseptic detoxic seedling in 40 days afterwards.
4. the large-scale planting new method of a kind of David's-harp high yield and high quality according to claim 1; it is characterized in that step (3) described condition of culture is: temperature 25 DEG C; within first 7 days, cultivate under half-light; turning intensity of illumination after 7 days is 2000Lx, within illumination 12h, dark 12h Cyclic culture 40-50 days, obtains propagation stem tuber.
5. the large-scale planting new method of a kind of David's-harp high yield and high quality according to claim 1; it is characterized in that step (4) described condition of culture is: temperature 25 DEG C; intensity of illumination is 2000Lx, within illumination 12h, dark 12h Cyclic culture 30-40 days, can obtain band bud, be with root to be good for the sealwort seedling of shape.
CN201610019921.1A 2016-01-13 2016-01-13 Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method Pending CN105532471A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610019921.1A CN105532471A (en) 2016-01-13 2016-01-13 Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610019921.1A CN105532471A (en) 2016-01-13 2016-01-13 Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method

Publications (1)

Publication Number Publication Date
CN105532471A true CN105532471A (en) 2016-05-04

Family

ID=55812479

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610019921.1A Pending CN105532471A (en) 2016-01-13 2016-01-13 Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method

Country Status (1)

Country Link
CN (1) CN105532471A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106489740A (en) * 2016-11-29 2017-03-15 宁夏农林科学院 A kind of seedling method for quickly breeding with polygonatum sibiricum Redoute bulb as explant
CN106888968A (en) * 2017-02-15 2017-06-27 中国计量大学 A kind of method of polygonatum filipes tissue culture and rapid proliferation
CN107027627A (en) * 2017-04-20 2017-08-11 宁德师范学院 A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE
CN107047298A (en) * 2017-02-15 2017-08-18 中国计量大学 A kind of method of David's-harp tissue culture and rapid proliferation
CN108541594A (en) * 2018-06-28 2018-09-18 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 A kind of tissue culture and rapid propagation method of polygonatum cirrhifolium Royle
CN109302983A (en) * 2017-07-26 2019-02-05 湖南省林业科学院 It is a kind of using terminal bud as the polygonatum cyrtonema rapid propagation method of explant
CN111109032A (en) * 2020-02-11 2020-05-08 沿河湘农中药材科技开发有限公司 Planting method of polygonatum sibiricum
CN111937703A (en) * 2020-08-24 2020-11-17 三明市农业科学研究院 Method for breeding high-quality polygonatum cyrtonema seedlings

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550413A (en) * 2011-12-26 2012-07-11 福建省三明市农业科学研究所 Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua
CN103039364A (en) * 2013-01-17 2013-04-17 河南师范大学 Method for circularly inducing micro tubers of rhizoma dioscoreae
CN104012405A (en) * 2013-02-28 2014-09-03 江南大学 Cultivation method of rhizoma corydalis tubers
CN105010135A (en) * 2015-06-29 2015-11-04 贵州信邦中药材发展有限公司 Tissue culture and rapid propagation method of Polygonatum sibiricum

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550413A (en) * 2011-12-26 2012-07-11 福建省三明市农业科学研究所 Rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua
CN103039364A (en) * 2013-01-17 2013-04-17 河南师范大学 Method for circularly inducing micro tubers of rhizoma dioscoreae
CN104012405A (en) * 2013-02-28 2014-09-03 江南大学 Cultivation method of rhizoma corydalis tubers
CN105010135A (en) * 2015-06-29 2015-11-04 贵州信邦中药材发展有限公司 Tissue culture and rapid propagation method of Polygonatum sibiricum

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
周建金等: "多花黄精组培快繁技术研究", 《福建农业科技》 *
周新华等: "多花黄精组培生根技术研究", 《经济林研究》 *
尹宏等: "黄精无性系建立的研究", 《西南农业学报》 *
汤盛杰等: "地藏黄精组培技术研究", 《安徽农学通报》 *
牟小翎等: "泰山野生黄精的组培快繁技术研究", 《山东农业科学》 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106489740A (en) * 2016-11-29 2017-03-15 宁夏农林科学院 A kind of seedling method for quickly breeding with polygonatum sibiricum Redoute bulb as explant
CN106888968A (en) * 2017-02-15 2017-06-27 中国计量大学 A kind of method of polygonatum filipes tissue culture and rapid proliferation
CN107047298A (en) * 2017-02-15 2017-08-18 中国计量大学 A kind of method of David's-harp tissue culture and rapid proliferation
CN107047298B (en) * 2017-02-15 2019-03-12 中国计量大学 A kind of method of polygonatum cyrtonema tissue culture and rapid proliferation
CN106888968B (en) * 2017-02-15 2019-06-28 中国计量大学 A kind of method of polygonatum filipes tissue culture and rapid proliferation
CN107027627A (en) * 2017-04-20 2017-08-11 宁德师范学院 A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE
CN107027627B (en) * 2017-04-20 2020-04-24 宁德师范学院 Microtuber propagation method for young embryo culture of polygonatum cyrtonema
CN109302983A (en) * 2017-07-26 2019-02-05 湖南省林业科学院 It is a kind of using terminal bud as the polygonatum cyrtonema rapid propagation method of explant
CN108541594A (en) * 2018-06-28 2018-09-18 广西壮族自治区农业科学院农产品质量安全与检测技术研究所 A kind of tissue culture and rapid propagation method of polygonatum cirrhifolium Royle
CN111109032A (en) * 2020-02-11 2020-05-08 沿河湘农中药材科技开发有限公司 Planting method of polygonatum sibiricum
CN111937703A (en) * 2020-08-24 2020-11-17 三明市农业科学研究院 Method for breeding high-quality polygonatum cyrtonema seedlings

Similar Documents

Publication Publication Date Title
CN105532471A (en) Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method
CN103004445B (en) Method for directly seedling dendrobe seedlings by using plastic greenhouse
CN104429965B (en) The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture
CN103688854B (en) The tissue culture and rapid propagation method of high eyebrow roxburgh anoectochilus terminal bud
CN102301951B (en) Method for rapidly propagating roots of subprostrate sophora by tissue culture
CN104041412B (en) The quick breeding method for tissue culture of a kind of Guizhou half capsule lettuce tongue
CN103535281B (en) Tissue culture medium of sealwort roots and stems and in-vitro regeneration method
CN104067939A (en) Tissue culture rapid propagation method of radix gentianae
CN105123529A (en) Rapid propagation and efficient cultivation method of Bletilla striata
CN106376328A (en) Wild bletila striata seed quick breeding method
CN105340739A (en) Oldenlandia diffusa tissue culture seedling rapid breeding method
CN105613234A (en) Direct seeding method of bletilla ochracea protocorm
CN108934679B (en) Root-cutting seedling raising and seedling tube raising method for aralia elata seem
CN111226789A (en) Bud multiplication medium for polygonatum cyrtonema tissue culture and polygonatum cyrtonema seedling culture method
CN105706872A (en) Bletilla striata seed direct seeding natural reproduction seedling method
CN110036851B (en) Pinellia ternata water-drought segmented seed breeding method
CN110574665B (en) Outdoor transplanting method for sealwort tissue culture seedlings
CN105340708A (en) Industrialized seedling production method of panax notoginseng
CN103548695B (en) A kind of meadowrueleaf corydalis root quick breeding method for tissue culture
CN104303765B (en) The high-yield planting method of the stem of noble dendrobium
CN105993956A (en) Fast propagating method for atractylis lancea
CN102845238A (en) Yingde black tea No 9 bud-stock grafting propagation method
CN104839023A (en) Method for cultivating dendrobium officinale
CN105104197A (en) Method for culturing and breeding dendrobium guangxiense tissue
CN106212229B (en) A kind of mating system of the extremely resistance to bolting radish germplasm in Shandong District

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20160504