CN105532472A - Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes - Google Patents
Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes Download PDFInfo
- Publication number
- CN105532472A CN105532472A CN201610027593.XA CN201610027593A CN105532472A CN 105532472 A CN105532472 A CN 105532472A CN 201610027593 A CN201610027593 A CN 201610027593A CN 105532472 A CN105532472 A CN 105532472A
- Authority
- CN
- China
- Prior art keywords
- culture
- cymbidium
- seed
- seedling
- stock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention relates to the technical field of plant culture in vitro and discloses a direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes. The direct induction seedling development method includes the following steps of material source screening, explant treatment, seed culture and seedling development induction culture. The problems that traditional cymbidium breeding mainly aims at division propagation, the division propagation coefficient is low, and large-scale production is difficult to achieve are solved. By means of a tissue culture method, the cymbidium propagation coefficient can be increased, a large number of test tube seedlings can be produced within a short period, and the industrial production requirement of cymbidium is met. Culture parameters and steps are verified through experiments, multiple root induction seedling development steps in traditional terrestrial orchid tissue culture are optimized, the rhizomes are induced into sprouts and roots at a time to form newborn plants, the induction process is reduced, induction time is shortened, production cost is remarkably reduced, and a corresponding culture method and technological parameters are disclosed.
Description
Technical field
The present invention relates to Vitro Plant culture technique field, after specifically Cymbidium lianpan plant seed axenic germination formation root-like stock, property cultivates the method for seedling again, and particularly a kind of hybridization Cymbidium lianpan root-like stock directly induces seedling establishment method.
Background technology
Medicinal herbs and Yunnan lotus are all the outstanding kinds of Cymbidium Cymbidium lianpan plant, and the two plant is strong, flower delicate fragrance, and ornamental value is high, are important orchid fine germplasm resources.Two cymbidium varieties fecund, in northwestern Yunnan Province and South Sichuan and two places neighboring region therewith, is good potted plant floral material and hybrid strain.
Cymbidium seed is very little, includes the globular embryo of some ateliosis, without endosperm, is difficult to sprout, need cultivates one's ability succeed with blue bacterium symbiosis or aseptic condition under nature.The sprouting of Cymbidium (Cmbidium) plant seed, existing people did research, but the hybridization of above epidendrum, axenic germination and root-like stock induction seedling have no report.
Summary of the invention
A kind of hybridization Cymbidium lianpan root-like stock is the object of the present invention is to provide directly to induce seedling establishment method, overcome all drawbacks that orchid Traditional breeding processes brings, be conducive to the reproduction coefficient expanding orchid, the test tube seedling of enormous amount can be produced in a short time, effectively meet the great demand that orchid industryization is produced.
For realizing above-mentioned technical purpose, reach above-mentioned technique effect, the invention discloses a kind of hybridization Cymbidium lianpan root-like stock and directly induce seedling establishment method, induction seedling establishment method includes following steps:
Material source screens: medicinal herbs and the strain of Yunnan Holland are Wild plant, cultivates to reach ninety percent ripe to fruit in resource garden, wins when pericarp shows slightly yellow;
Explant process: by above-mentioned nine ripening fruitss with 75% alcohol disinfecting 30-60 second, proceed to 0.1% mercuric chloride surface sterilization 8-12 minute, aseptic water washing 3-4 time, desinfection chamber takes out seed and after binding up with gauze seed, with sterile water rinse 5 times, then uses 0.1mol/LKOH solution pretreatment 8-10 minute, with aseptic water washing 3 times, with 20% hypochlorite disinfectant 15-20 minute, after aseptic water washing 5 times, be inoculated into the MS solid culture medium of improvement;
Seed culture: in whole incubation, seed asepsis sprouting adopts camera bellows to cultivate, being cultured to seed germination is that ball stem becomes during root-like stock to cultivate employing illumination cultivation, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800-2500 lux (lx), illumination every day 12-15 hour, is transferred to another blake bottle until seed germination after growing root-like stock, carries out seedling Fiber differentiation;
Seedling Fiber differentiation: use the improvement MS solid inducing culture of the growth hormone NAA of the basic element of cell division 6-BA and 0.2-2.0mg/L containing 0.5-5.0mg/L to cultivate, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800-2500 lux (lx), illumination every day 12-15 hour, grow bud and Xin Gen after 2-5 months, form healthy and strong plant.
Preferably, the MS solid culture medium improved in explant process is, the improvement MS solid culture medium of the growth hormone NAA of the basic element of cell division 6-BA and 1.0-2.0mg/L containing 1.0-5.0mg/L, wherein medium pH 5.6, agar 4.0-6.5g/L.
Preferably, improve in seedling Fiber differentiation in MS solid inducing culture and also include mashed potatoes 50g/L and albumen powder 0.2g/L.
The present invention has following beneficial effect:
1. the present invention is cultivated by strict seed selection, optimization; overcoming traditional orchid breeds main based on division propagation; and division propagation coefficient is low; the ratio of every old seedling and newborn seedling is only about 1:1 ~ 3; be difficult to the problem of carrying out large-scale production; the method that have employed tissue cultures is conducive to the reproduction coefficient expanding orchid, can produce the test tube seedling of enormous amount in a short time, effectively meets the great demand that orchid industryization is produced.
2. through lot of experiment validation culture parameters and incubation step, multistep root induction seedling step in traditional Terrestrial orchid group training is optimized adjustment, the present invention is from the disposable induction buds sprouting of root-like stock and root, become nascent plant, decrease Induction Process and shorten induction time, significantly reduce production cost, and disclose corresponding breeding method and technological parameter.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.
Embodiment 1
The invention discloses a kind of hybridization Cymbidium lianpan root-like stock and directly induce seedling establishment method, induction seedling establishment method includes following steps:
Material source screens: medicinal herbs and the strain of Yunnan Holland are field and adopt, and cultivates to reach ninety percent ripe to fruit in resource garden, wins when pericarp shows slightly yellow;
Explant process: by above-mentioned nine ripening fruitss 75% alcohol disinfecting 30 seconds, proceed to 0.1% mercuric chloride surface sterilization 8 minutes, aseptic water washing 3 times, desinfection chamber takes out seed and after binding up with gauze seed, with sterile water rinse 5 times, then uses 0.1mol/LKOH solution pretreatment 8 minutes, with aseptic water washing 3 times, with 20% hypochlorite disinfectant 15 minutes, after aseptic water washing 5 times, be inoculated into the MS solid culture medium of improvement.Wherein, the MS solid culture medium of improvement is, the improvement MS solid culture medium of the growth hormone NAA of the basic element of cell division 6-BA and 1.0mg/L containing 1.0mg/L, wherein medium pH 5.6, agar 4.0g/L;
Seed culture: in whole incubation, seed asepsis sprouting adopts camera bellows to cultivate, being cultured to seed germination is that ball stem becomes during root-like stock to cultivate employing illumination cultivation, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800 lux (lx), illumination every day 12 hours, is transferred to another blake bottle until seed germination after growing root-like stock, carries out seedling Fiber differentiation;
Seedling Fiber differentiation: use growth hormone NAA, the mashed potatoes 50g/L of the basic element of cell division 6-BA, the 0.2mg/L containing 0.5mg/L and the improvement MS solid inducing culture of albumen powder 0.2g/L to cultivate, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800 lux (lx), illumination every day 12 hours, grow bud and Xin Gen after 2-5 month, form healthy and strong plant.
Embodiment 2
The invention discloses a kind of hybridization Cymbidium lianpan root-like stock and directly induce seedling establishment method, induction seedling establishment method includes following steps:
Material source screens: medicinal herbs and the strain of Yunnan Holland are field and adopt, and cultivates to reach ninety percent ripe to fruit in resource garden, wins when pericarp shows slightly yellow;
Explant process: by above-mentioned nine ripening fruitss 75% alcohol disinfecting 60 seconds, proceed to 0.1% mercuric chloride surface sterilization 12 minutes, aseptic water washing 4 times, desinfection chamber takes out seed and after binding up with gauze seed, with sterile water rinse 5 times, then uses 0.1mol/LKOH solution pretreatment 10 minutes, with aseptic water washing 3 times, with 20% hypochlorite disinfectant 20 minutes, after aseptic water washing 5 times, be inoculated into the MS solid culture medium of improvement.Wherein, the MS solid culture medium of improvement is, the improvement MS solid culture medium of the growth hormone NAA of the basic element of cell division 6-BA and 2.0mg/L containing 5.0mg/L, wherein medium pH 5.6, agar 6.5g/L;
Seed culture: in whole incubation, seed asepsis sprouting adopts camera bellows to cultivate, being cultured to seed germination is that ball stem becomes during root-like stock to cultivate employing illumination cultivation, condition of culture is: temperature 25 ± 2 DEG C, illumination 2500 lux (lx), illumination every day 15 hours, is transferred to another blake bottle until seed germination after growing root-like stock, carries out seedling Fiber differentiation;
Seedling Fiber differentiation: use growth hormone NAA, the mashed potatoes 50g/L of the basic element of cell division 6-BA, the 2.0mg/L containing 5.0mg/L and the improvement MS solid inducing culture of albumen powder 0.2g/L to cultivate, (lacking condition of culture), condition of culture is: temperature 25 ± 2 DEG C, illumination 2500 lux (lx), illumination every day 15 hours, grow bud and Xin Gen after 2-5 month, form healthy and strong plant.
The above; be only the present invention's preferably embodiment, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.
Claims (3)
1. hybridize Cymbidium lianpan root-like stock and directly induce a seedling establishment method, it is characterized in that, described induction seedling establishment method includes following steps:
Material source screens: medicinal herbs and the strain of Yunnan Holland are Wild plant, cultivates to reach ninety percent ripe to fruit in resource garden, wins when pericarp shows slightly yellow;
Explant process: by above-mentioned nine ripening fruitss with 75% alcohol disinfecting 30-60 second, proceed to 0.1% mercuric chloride surface sterilization 8-12 minute, aseptic water washing 3-4 time, desinfection chamber takes out seed and after binding up with gauze seed, with sterile water rinse 5 times, then uses 0.1mol/LKOH solution pretreatment 8-10 minute, with aseptic water washing 3 times, with 20% hypochlorite disinfectant 15-20 minute, after aseptic water washing 5 times, be inoculated into the MS solid culture medium of improvement;
Seed culture: in whole incubation, seed asepsis sprouting adopts camera bellows to cultivate, being cultured to seed germination is that ball stem becomes during root-like stock to cultivate employing illumination cultivation, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800-2500 lux (lx), illumination every day 12-15 hour, is transferred to another blake bottle until seed germination after growing root-like stock, carries out seedling Fiber differentiation;
Seedling Fiber differentiation: use the improvement MS solid inducing culture of the growth hormone NAA of the basic element of cell division 6-BA and 0.2-2.0mg/L containing 0.5-5.0mg/L to cultivate, condition of culture is: temperature 25 ± 2 DEG C, illumination 1800-2500 lux (lx), illumination every day 12-15 hour, grow bud and Xin Gen after 2-5 months, form healthy and strong plant.
2. a kind of hybridization Cymbidium lianpan root-like stock as claimed in claim 1 directly induces seedling establishment method, it is characterized in that: the MS solid culture medium of the improvement described in explant process is, the improvement MS solid culture medium of the growth hormone NAA of the basic element of cell division 6-BA and 1.0-2.0mg/L containing 1.0-5.0mg/L, wherein medium pH 5.6, agar 4.0-6.5g/L.
3. a kind of hybridization Cymbidium lianpan root-like stock as claimed in claim 1 or 2 directly induces seedling establishment method, it is characterized in that: also include mashed potatoes 50g/L and albumen powder 0.2g/L in the improvement MS solid inducing culture described in seedling Fiber differentiation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027593.XA CN105532472A (en) | 2016-01-16 | 2016-01-16 | Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027593.XA CN105532472A (en) | 2016-01-16 | 2016-01-16 | Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105532472A true CN105532472A (en) | 2016-05-04 |
Family
ID=55812480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610027593.XA Pending CN105532472A (en) | 2016-01-16 | 2016-01-16 | Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105532472A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122325A (en) * | 2018-11-09 | 2019-01-04 | 翁源县天下泽雨农业科技有限公司 | A kind of aseptic seeding quick-breeding method of sword-leaved cymbidium seed |
| CN112136687A (en) * | 2020-10-28 | 2020-12-29 | 中国科学院昆明植物研究所 | Rapid propagation and in-vitro preservation method of saprophytic orchid |
| CN115589944A (en) * | 2022-08-25 | 2023-01-13 | 云南省农业科学院花卉研究所（Cn） | Method for culturing cymbidium tortisepalum plants on large scale |
-
2016
- 2016-01-16 CN CN201610027593.XA patent/CN105532472A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109122325A (en) * | 2018-11-09 | 2019-01-04 | 翁源县天下泽雨农业科技有限公司 | A kind of aseptic seeding quick-breeding method of sword-leaved cymbidium seed |
| CN112136687A (en) * | 2020-10-28 | 2020-12-29 | 中国科学院昆明植物研究所 | Rapid propagation and in-vitro preservation method of saprophytic orchid |
| CN115589944A (en) * | 2022-08-25 | 2023-01-13 | 云南省农业科学院花卉研究所（Cn） | Method for culturing cymbidium tortisepalum plants on large scale |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103416305B (en) | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings | |
| CN101889547B (en) | Aseptic and rapid propagation method of dendrobium devonianum seeds | |
| CN104082123A (en) | Cultivation method of tetraploid Anoectochilus roxburghii | |
| CN102499088B (en) | Method for quickly breeding seedlings of Guangxi anoectochilus roxburghii capsules by utilizing Guangxi anoectochilus roxburghii capsules | |
| CN101822220A (en) | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii | |
| CN106376328A (en) | Wild bletila striata seed quick breeding method | |
| CN104429962A (en) | Cultivation method of dendrobium nobile tissue culture seedlings | |
| CN103766222A (en) | Stevia rebaudiana tissue culture method and culture medium thereof | |
| CN104160956A (en) | High efficient and fast culture method of anoectochilus roxburghii tissue cultured seedling | |
| WO2021077755A1 (en) | Method for sterilizing budded stem of kadsura coccinea and rapid proliferation method therefor | |
| CN101897297B (en) | Two-step tissue culture quick propagation method for hemerocallis | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN1320853C (en) | Method for breeding precious cross bred orchid | |
| CN106258960B (en) | A kind of orchid seed sprouting quick-breeding method | |
| CN105532472A (en) | Direct induction seedling development method for hybrid cymbidium tortisepalum rhizomes | |
| CN107079817A (en) | Pocket Lanzhou and Xinjiang kind " excellent pocket is blue " tissue culture and rapid propagation method | |
| CN105191803A (en) | Dendrobium officinale tissue culture bag seedling production method | |
| CN105028206A (en) | Tissue culture propagation method for Hainan dendrobe | |
| CN103039360B (en) | Method for quickly propagating leeka through tissue culture | |
| CN104813931A (en) | Tissue culture and rapid propagation method for Dendrobium officinale | |
| CN104686334A (en) | Tissue culture and rapid propagation method for androsace longifolia | |
| CN1203754C (en) | Breeding technology of konjak testtube taro | |
| CN102726288A (en) | Method for culturing and rapidly propagating large-flowered cymbidiums | |
| CN103125392A (en) | Dendrobium officinale sterile seed sowing ultrafast propagation method | |
| CN105594596A (en) | Tissue culture method for strawberry virus-free and rapid propagation for large-scale production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160504 |