CN103125392A - Dendrobium officinale sterile seed sowing ultrafast propagation method - Google Patents
Dendrobium officinale sterile seed sowing ultrafast propagation method Download PDFInfo
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- CN103125392A CN103125392A CN2013100762994A CN201310076299A CN103125392A CN 103125392 A CN103125392 A CN 103125392A CN 2013100762994 A CN2013100762994 A CN 2013100762994A CN 201310076299 A CN201310076299 A CN 201310076299A CN 103125392 A CN103125392 A CN 103125392A
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Abstract
The invention discloses a dendrobium officinale sterile seed sowing ultrafast propagation method, and belongs to the technical field of bioengineering. The preparation method comprises two steps of seed germination culture and rooting culture. According to the preparation method disclosed by the invention, culture processes are reduced, and only two culture periods are required in whole process; the culture duration is reduced, only 6-7 months are spent from seed sowing to seedling, and time, labor and cost are saved; and planting survival rate of the cultured strong seedling is high, which is greater than 93%.
Description
Technical field
The present invention relates to a kind of dendrobium candidum axenic and broadcast ultrafast propagation method, belong to technical field of bioengineering.
Background technology
The stem of noble dendrobium is Chinese traditional rare Chinese medicine, is divided into the kinds such as iron sheet, yellow grass, Jin Chai, Huoshan, ring grass, horsewhip, and wherein dendrobium candidum is of the best quality, and mainly is distributed in the south of the River and southwestern each province.The dendrobium candidum medical value is high, and health care is strong, has nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, makes an uproar and make eye bright, strengthen human immunity Lixing effect clearly, can be used for the pyreticosis wound when, dry polydipsia, abnormal heat row various disease conditions after being ill.Dendrobium candidum grows on overhanging cliff more, and is minimum due to seed, without endosperm, and sprouting under natural conditions needs and something mycosymbiosis; reproduction rate is extremely low; and the medicinal herb grower excavates all the year round in a large number because of the producing region, and resource is endangered, and country has classified it as the rareness species of focused protection.Some units and scientific research personnel's dendrobium candidum have carried out the research of artificial propagation techniques at present, but operation is many, and the cycle is long, ten to 11 months even one-year ages, and the time-consuming cost of taking a lot of work is high.
Summary of the invention
Many in order to overcome the prior art operation, the cycle is long, and the defective that cost is high reaches efficiently purpose fast, the invention provides a kind of dendrobium candidum axenic and broadcasts ultrafast propagation method.
Technical scheme is: a kind of dendrobium candidum axenic is broadcast ultrafast propagation method, is comprised of following steps:
A, seed germination are cultivated
Under aseptic condition, to evenly be sown in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.3～0.4mg/L+16～18%V/V banana pulp through the seed of sterilization, first three sky does not supply light, intensity of illumination 1200～1500Ix after three days, illumination every day 8～9 hours, 24～27 ℃ of cultivation temperature, incubation time 90～100 days, seed progressively expands, and grows plumule, leaf bud;
B, strengthening seedling and rooting are cultivated
The seedling of 2-3 sheet leaf bud will be grown to, be transferred in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.2～0.3mg/L+14～16%V/V banana pulp, intensity of illumination 1800～2600Ix, 25～28 ℃ of cultivation temperature, incubation time 90～100 days, Seedling Differentiation grows into 2-3 bar root, 4 leaves, 2.5 centimetres of high plant, namely can be moved to outdoor hardening, transplanting.
Advantage of the present invention is:
1, reduced the cultivation operation, omnidistance only with 2 culture periods;
2, shortened incubation time, broadcast seedling from son and only have 6～7 months, saving of work and time is saved cost;
In the strong sprout of 3, cultivating, planting survival rates is high, can reach more than 93%.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1
Under aseptic condition, to evenly be sown in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.3mg/L+16%V/V banana pulp through the seed of sterilization, first three sky does not supply light, after three days, intensity of illumination progressively increases to 1500Ix by 1200Ix, illumination every day 9 hours, 25 ℃ of cultivation temperature, incubation time 100 days, seed progressively expands, and grows plumule, leaf bud; The seedling of 2-3 sheet leaf bud will be grown to, be transferred in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.2/L+16%V/V banana pulp, intensity of illumination 2200Ix, 28 ℃ of cultivation temperature, incubation time 90 days, Seedling Differentiation grows into 2-3 bar root, 4 leaves, 2.5 centimetres of high plant, namely can be moved to outdoor hardening, transplanting.
Embodiment 2
Under aseptic condition, to evenly be sown in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.4mg/L+18%V/V banana pulp through the seed of sterilization, first three sky does not supply light, after three days, intensity of illumination progressively increases to 1500Ix by 1200Ix, illumination every day 8 hours, 26 ℃ of cultivation temperature, incubation time 90 days, seed progressively expands, and grows plumule, leaf bud; The seedling of 2-3 sheet leaf bud will be grown to, be transferred in the medium of MS minimal medium+a-methyl α-naphthyl acetate (NAA) 0.2/L+14%V/V banana pulp, intensity of illumination 2600Ix, 26 ℃ of cultivation temperature, incubation time 100 days, Seedling Differentiation grows into 2-3 bar root, 4 leaves, 2.5 centimetres of high plant, namely can be moved to outdoor hardening, transplanting.
Claims (1)
1. dendrobium candidum axenic is broadcast ultrafast propagation method, it is characterized in that being comprised of following steps:
A, seed germination are cultivated
Under aseptic condition, to evenly be sown in the medium of MS minimal medium+a-methyl α-naphthyl acetate 0.3～0.4mg/L+16～18%V/V banana pulp through the seed of sterilization, first three sky does not supply light, intensity of illumination 1200～1500Ix after three days, illumination every day 8～9 hours, 24～27 ℃ of cultivation temperature, incubation time 90～100 days, seed progressively expands, and grows plumule, leaf bud;
B, strengthening seedling and rooting are cultivated
The seedling of 2-3 sheet leaf bud will be grown to, be transferred in the medium of MS minimal medium+a-methyl α-naphthyl acetate 0.2～0.3mg/L+14～16%V/V banana pulp, intensity of illumination 1800～2600Ix, 25～28 ℃ of cultivation temperature, incubation time 90～100 days, Seedling Differentiation grows into 2-3 bar root, 4 leaves, 2.5 centimetres of high plant, namely can be moved to outdoor hardening, transplanting.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100762994A CN103125392A (en) | 2013-03-08 | 2013-03-08 | Dendrobium officinale sterile seed sowing ultrafast propagation method |
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| CN2013100762994A CN103125392A (en) | 2013-03-08 | 2013-03-08 | Dendrobium officinale sterile seed sowing ultrafast propagation method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104429972A (en) * | 2014-12-25 | 2015-03-25 | 安徽同创现代农业投资发展有限公司 | Explant induction culture medium for dendrobium officinale tissue culture seedling culture |
| CN104429973A (en) * | 2014-12-25 | 2015-03-25 | 安徽同创现代农业投资发展有限公司 | Method of culturing dendrobium officinale plantlets |
| CN104542270A (en) * | 2013-10-14 | 2015-04-29 | 厦门塔斯曼生物工程有限公司 | Rapid propagation method for medicinal dendrobium nobile |
| CN107251728A (en) * | 2017-05-09 | 2017-10-17 | 贵州济生农业科技有限公司 | A kind of extracting method of dendrobium candidum |
Citations (3)
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| CN1961632A (en) * | 2006-12-08 | 2007-05-16 | 蔡仲玉 | An officinal dendrobium stem plantation method |
| CN101843218A (en) * | 2010-05-06 | 2010-09-29 | 谢艾明 | Emulated wild zoology planting method for Dendrobium officinal |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
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2013
- 2013-03-08 CN CN2013100762994A patent/CN103125392A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961632A (en) * | 2006-12-08 | 2007-05-16 | 蔡仲玉 | An officinal dendrobium stem plantation method |
| CN101933456A (en) * | 2009-06-29 | 2011-01-05 | 西双版纳增靓生物科技有限公司 | Method for quickly breeding seedlings of dendrobium officinale capsule |
| CN101843218A (en) * | 2010-05-06 | 2010-09-29 | 谢艾明 | Emulated wild zoology planting method for Dendrobium officinal |
Non-Patent Citations (1)
| Title |
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| 洪香娇等: "铁皮石斛的组织培养与快速繁殖", 《现代园艺》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542270A (en) * | 2013-10-14 | 2015-04-29 | 厦门塔斯曼生物工程有限公司 | Rapid propagation method for medicinal dendrobium nobile |
| CN104429972A (en) * | 2014-12-25 | 2015-03-25 | 安徽同创现代农业投资发展有限公司 | Explant induction culture medium for dendrobium officinale tissue culture seedling culture |
| CN104429973A (en) * | 2014-12-25 | 2015-03-25 | 安徽同创现代农业投资发展有限公司 | Method of culturing dendrobium officinale plantlets |
| CN107251728A (en) * | 2017-05-09 | 2017-10-17 | 贵州济生农业科技有限公司 | A kind of extracting method of dendrobium candidum |
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Application publication date: 20130605 |