CN105493861A - Method for directly seeding bletilla striata protocorms - Google Patents
Method for directly seeding bletilla striata protocorms Download PDFInfo
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- CN105493861A CN105493861A CN201510989975.6A CN201510989975A CN105493861A CN 105493861 A CN105493861 A CN 105493861A CN 201510989975 A CN201510989975 A CN 201510989975A CN 105493861 A CN105493861 A CN 105493861A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a method for directly seeding bletilla striata protocorms, wherein bletilla striata seeds grow to be protocorms in the conditions of tissue culture, and then the protocorms are directly seed in a greenhouse after tissue culture. The method for directly seeding the bletilla striata protocorms is simple and easy, enables seeds to rapidly germinate in the conditions of tissue culture firstly, and then transplants the seeds in a later period in the nature when the protocorms need to transfer and culture, thereby improving germination rate, reducing production cost, being capable of obtaining a large number of bletilla striata seeds in short time, meeting the demands of large area and large-scale bletilla striata seeding, and having dual significances on production and economy.
Description
Technical field
The invention belongs to medicinal plant artificial planting technique field, be specifically related to a kind of live mating system of protocorm of national secondary endangered medicinal plant bletilla.
Background technology
Bletilla (Bletillastriata) belongs to herbaceos perennial for the orchid family bletilla, is country 2 grades of endangered plants, is mainly distributed in the areas such as the Guizhou on the south China Qinling Mountains, Hubei, Southern Shaanxi.In " Chinese Plants will ", bletilla platymiscium has four kinds, is respectively magnificent bletilla, little bletilla, bletilla and chrysanthemum bletilla." 2015 editions pharmacopeia " regulation bletilla is used as medicine with dry tuber, has the effect of astringing to arrest bleeding, nourishing generate fluid, myogenic pain relieving.Be widely used in the illnesss such as treatment hemoptysis, haematemesis, traumatism and bleeding, sore swollen toxin, pulmonary tuberculosis hemoptysis, ulcer haemorrhage clinically.
Have 3 ~ 50,000, seed in bletilla fruit pod, seed under field conditions (factors) germination rate is low, therefore, solves bletilla shortage of resources also mainly through tissue culture mode.Tissue culture technique is ripe, but it but also exists environmental requirement is strict, cost is high feature.The research group at inventor place is by related art method, and at present by bletilla live success under field conditions (factors), although greatly reduce production cost, needed for from seed to seedling, growth cycle is longer, the general time needing more than 10 months.
Summary of the invention
Technical problem to be solved by this invention is to overcome existing bletilla plantation Problems existing, provides that a kind of production cost is low, germination rate is high and can obtain the method for a large amount of bletilla seedling at short notice.
Solve the problems of the technologies described above adopted technical scheme to be made up of following step:
1, bletilla seed germination
Bletilla seed is sowed on the bletilla medium in tissue culture bottle, temperature be 25 ± 2 DEG C, the tissue culture room of intensity of illumination 2000 ~ 3000Lx, illumination 12h/12h cultivates 65 ~ 70 days, seed germination is made to become protocorm, then tissue culture bottle to be moved on greenhouse seedbed hardening process one week, protocorm is taken out, with water by clean for the medium treatment adhered to above.
2, seedbed process
Select the seedbed of intelligent greenhouse, seedbed height is 1 ~ 1.5m, and waddy baffle is used every 2 meters in seedbed, is laid in by plastic paper on seedbed, and the surrounding of plastic paper is fixed on edge, seedbed, guarantees seedbed can keep the water that 2cm is high.
3, matrix treatments
Be 150 ~ 250:150 ~ 250:1 mixing by German Klasmann raw fiber peat soil TS1, coconut palm chaff, tpn according to volume ratio, be mixed with matrix, the matrix prepared is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, square plate is placed on seedbed, waters to matrix, matrix is thoroughly irrigated.
4, protocorm sowing
Using the protocorm after the process of step 1 hardening as kind, according to line-spacing 2 ~ 3cm, spacing in the rows 1 ~ 2cm, press from both sides gently to stromal surface with tweezers, sow rear preservative film and cover square plate, the temperature controlled in booth is 20 ~ 25 DEG C, relative moisture is 40% ~ 60%, and keep ventilating in booth, watered once by the space between square plate and square plate every 3 ~ 5 days after sowing one week.
5, to topdress management
Treat that bletilla seedling grows two panels true leaf, throw off preservative film, sprayed blue precious nutrient solution and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days respectively.
Above-mentioned bletilla medium is in MS medium, add 6-benzyl aminoadenine, methyl α-naphthyl acetate, bananas juice, use distilled water constant volume, then NaOH aqueous solution adjust ph to 5.8 ~ 6.0 of 0.1mol/L are used, 121 DEG C of sterilizings 20 minutes, be mixed with bletilla medium, wherein said bananas juice 100g banana is added 100mL water in juice extractor beat obtained, and in bletilla medium, the concentration of 6-benzyl aminoadenine is 1.5 ~ 2.5mg/L, banana concentration is 75 ~ 120g/L, concentration of NAA is 0.3 ~ 0.8mg/L.
In above-mentioned steps 3, preferably by German Klasmann raw fiber peat soil TS1, coconut palm chaff, tpn according to volume ratio be 200:200:1 mixing, be mixed with matrix.
Bletilla condition of tissue culture is very ripe; to sow in tissue culture bottle about 70 days at seed, seed can be sprouted to protocorm, and root and the bud of part occur; the present invention passed through after by protocorm domestication in this stage; in conjunction with the ripe live system in greenhouse, it is live to carry out protocorm, both improves germination rate; and reduce production cost; a large amount of bletilla seedlings can be obtained at short notice, meet the needs of large area, the nursery of scale bletilla, there is the double meaning produced with economically.
Accompanying drawing explanation
Fig. 1 is the photo that in embodiment 1, bletilla protocorm sows bletilla seedling after 8 months.
Fig. 2 is the photo that in embodiment 2, bletilla protocorm sows bletilla seedling after 8 months.
Fig. 3 is the photo that in embodiment 3, bletilla protocorm sows bletilla seedling after 8 months.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in more detail, but protection scope of the present invention is not limited only to these embodiments.
Embodiment 1
1, bletilla seed germination
Bletilla seed is sowed on the bletilla medium in tissue culture bottle, temperature be 25 ± 2 DEG C, the tissue culture room of intensity of illumination 2500Lx, illumination 12h/12h cultivates 70 days, seed germination is made to become protocorm, then tissue culture bottle to be moved on greenhouse seedbed hardening process one week, part protocorm can produce fibrous root and bud, gently protocorm is taken out, with water by clean for the medium treatment adhered to above.Wherein bletilla medium is in MS medium, add 6-benzyl aminoadenine, methyl α-naphthyl acetate, bananas juice, use distilled water constant volume, then NaOH aqueous solution adjust ph to 5.8 ~ 6.0 of 0.1mol/L are used, 121 DEG C of sterilizings 20 minutes, be mixed with bletilla medium, described bananas juice 100g banana is added 100mL water in juice extractor beat obtained, and in bletilla medium, the concentration of 6-benzyl aminoadenine is 2.0mg/L, banana concentration is 100g/L, concentration of NAA is 0.5mg/L.
2, seedbed process
Select the seedbed of intelligent greenhouse, seedbed height is 1.5m, and seedbed is the square waddy baffle of 6cm every 2 meters with width, and be laid in by plastic paper on seedbed, the surrounding of plastic paper is fixed on edge, seedbed, guarantees seedbed can keep the water that 2cm is high.
3, matrix treatments
By German Klasmann raw fiber peat soil TS1 (being provided by Yan Jin flowers market, Xi'an rainbow matrix), coconut palm chaff, tpn according to volume ratio be 200:200:1 mixing, be mixed with matrix, the matrix prepared is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, square plate is placed on seedbed, waters to matrix, matrix is thoroughly irrigated.
4, protocorm sowing
Using the protocorm after the process of step 1 hardening as kind, according to line-spacing 2cm, spacing in the rows 1cm, press from both sides gently to stromal surface with tweezers, sow rear preservative film and cover square plate, the temperature controlled in booth is 20 ~ 25 DEG C, relative moisture is 40% ~ 60%, and keep ventilating in booth, watered once by the space between square plate and square plate every 5 days after sowing one week.
5, to topdress management
Sow after 1 month, the long full two panels true leaf of bletilla seedling, emergence rate, more than 95%, throws off preservative film, sprays blue precious nutrient solution and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days respectively.Sow after 5 months, bletilla plant all grows 3 ~ 4 leaves, plant height is generally more than 6cm, cultivate the standard of 8 ~ 10 months under having reached condition of tissue culture, as seen from Figure 1, sow after 8 months, bletilla plant is in down seedling stage, grow 4 ~ 5 leaves, plant height is generally more than 12cm, and bulb diameter generally can reach more than 2cm.
Embodiment 2
1, bletilla seed germination
Bletilla seed is sowed on the bletilla medium in tissue culture bottle, temperature be 25 ± 2 DEG C, the tissue culture room of intensity of illumination 3000Lx, illumination 12h/12h cultivates 70 days, seed germination is made to become protocorm, then tissue culture bottle to be moved on greenhouse seedbed hardening process one week, part protocorm can produce fibrous root and bud, gently protocorm is taken out, with water by clean for the medium treatment adhered to above.Wherein bletilla medium is in MS medium, add 6-benzyl aminoadenine, methyl α-naphthyl acetate, bananas juice, use distilled water constant volume, then NaOH aqueous solution adjust ph to 5.8 ~ 6.0 of 0.1mol/L are used, 121 DEG C of sterilizings 20 minutes, be mixed with bletilla medium, described bananas juice 100g banana is added 100mL water in juice extractor beat obtained, and in bletilla medium, the concentration of 6-benzyl aminoadenine is 2.5mg/L, banana concentration is 120g/L, concentration of NAA is 0.3mg/L.
2, seedbed process
Select the seedbed of intelligent greenhouse, seedbed height is 1m, and seedbed is the square waddy baffle of 6cm every 2 meters with width, and be laid in by plastic paper on seedbed, the surrounding of plastic paper is fixed on edge, seedbed, guarantees seedbed can keep the water that 2cm is high.
3, matrix treatments
By German Klasmann raw fiber peat soil TS1 (being provided by Yan Jin flowers market, Xi'an rainbow matrix), coconut palm chaff, tpn according to volume ratio be 250:150:1 mixing, be mixed with matrix, the matrix prepared is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, square plate is placed on seedbed, waters to matrix, matrix is thoroughly irrigated.
4, protocorm sowing
Using the protocorm after the process of step 1 hardening as kind, according to line-spacing 2cm, spacing in the rows 1cm, press from both sides gently to stromal surface with tweezers, sow rear preservative film and cover square plate, the temperature controlled in booth is 20 ~ 25 DEG C, relative moisture is 40% ~ 60%, and keep ventilating in booth, watered once by the space between square plate and square plate every 5 days after sowing one week.
5, to topdress management
Sow after 1 month, the long full two panels true leaf of bletilla seedling, emergence rate, more than 90%, throws off preservative film, sprays blue precious nutrient solution and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days respectively.Sow after 5 months, bletilla plant all grows 3 ~ 4 leaves, and plant height, generally more than 6cm, cultivates the standard of 8 ~ 10 months under having reached condition of tissue culture.As seen from Figure 2, sow after 8 months, bletilla plant is in down seedling stage, and part grows 4 ~ 5 leaves, and plant height is generally more than 12cm, and bulb diameter generally can reach more than 2cm.
Embodiment 3
1, bletilla seed germination
Bletilla seed is sowed on the bletilla medium in tissue culture bottle, temperature be 25 ± 2 DEG C, the tissue culture room of intensity of illumination 2000Lx, illumination 12h/12h cultivates 70 days, seed germination is made to become protocorm, then tissue culture bottle to be moved on greenhouse seedbed hardening process one week, part protocorm can produce fibrous root and bud, gently protocorm is taken out, with water by clean for the medium treatment adhered to above.Wherein bletilla medium is in MS medium, add 6-benzyl aminoadenine, methyl α-naphthyl acetate, bananas juice, use distilled water constant volume, then NaOH aqueous solution adjust ph to 5.8 ~ 6.0 of 0.1mol/L are used, 121 DEG C of sterilizings 20 minutes, be mixed with bletilla medium, described bananas juice 100g banana is added 100mL water in juice extractor beat obtained, and in bletilla medium, the concentration of 6-benzyl aminoadenine is 1.5mg/L, banana concentration is 75g/L, concentration of NAA is 0.8mg/L.
2, seedbed process
Select the seedbed of intelligent greenhouse, seedbed height is 1.5m, and seedbed is the square waddy baffle of 6cm every 2 meters with width, and be laid in by plastic paper on seedbed, the surrounding of plastic paper is fixed on edge, seedbed, guarantees seedbed can keep the water that 2cm is high.
3, matrix treatments
By German Klasmann raw fiber peat soil TS1 (being provided by Yan Jin flowers market, Xi'an rainbow matrix), coconut palm chaff, tpn according to volume ratio be 150:250:1 mixing, be mixed with matrix, the matrix prepared is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, square plate is placed on seedbed, waters to matrix, matrix is thoroughly irrigated.
4, protocorm sowing
Using the protocorm after the process of step 1 hardening as kind, according to line-spacing 2cm, spacing in the rows 1cm, press from both sides gently to stromal surface with tweezers, sow rear preservative film and cover square plate, the temperature controlled in booth is 20 ~ 25 DEG C, relative moisture is 40% ~ 60%, and keep ventilating in booth, watered once by the space between square plate and square plate every 5 days after sowing one week.
5, to topdress management
Sow after 1 month, the long full two panels true leaf of bletilla seedling, emergence rate, more than 85%, throws off preservative film, sprays blue precious nutrient solution and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days respectively.Sow after 5 months, bletilla plant all grows 3 ~ 4 leaves, and plant height, generally more than 6cm, cultivates the standard of 8 ~ 10 months under having reached condition of tissue culture.As seen from Figure 3, sow after 8 months, bletilla plant is in down seedling stage, and most plant grows 4 ~ 5 leaves, and plant height is commonly 10 ~ 12cm, and bulb diameter majority can reach more than 2cm.
Claims (3)
1. the method that bletilla protocorm is live, is characterized in that it is made up of following step:
(1) bletilla seed germination
Bletilla seed is sowed on the bletilla medium in tissue culture bottle, temperature be 25 ± 2 DEG C, the tissue culture room of intensity of illumination 2000 ~ 3000Lx, illumination 12h/12h cultivates 65 ~ 70 days, seed germination is made to become protocorm, then tissue culture bottle to be moved on greenhouse seedbed hardening process one week, protocorm is taken out, with water by clean for the medium treatment adhered to above;
(2) seedbed process
Select the seedbed of intelligent greenhouse, seedbed height is 1 ~ 1.5m, and waddy baffle is used every 2 meters in seedbed, is laid in by plastic paper on seedbed, and the surrounding of plastic paper is fixed on edge, seedbed, guarantees seedbed can keep the water that 2cm is high;
(3) matrix treatments
Be 150 ~ 250:150 ~ 250:1 mixing by German Klasmann raw fiber peat soil TS1, coconut palm chaff, tpn according to volume ratio, be mixed with matrix, the matrix prepared is loaded in the porose square plate in bottom, be filled to 2/3 place of the square plate degree of depth, flatten compacting, square plate is placed on seedbed, waters to matrix, matrix is thoroughly irrigated;
(4) protocorm sowing
Using the protocorm after step (1) hardening process as kind, according to line-spacing 2 ~ 3cm, spacing in the rows 1 ~ 2cm, press from both sides gently to stromal surface with tweezers, sow rear preservative film and cover square plate, the temperature controlled in booth is 20 ~ 25 DEG C, relative moisture is 40% ~ 60%, and keep ventilating in booth, watered once every 3 ~ 5 days by the space between square plate and square plate after sowing one week;
(5) to topdress management
Treat that bletilla seedling grows two panels true leaf, throw off preservative film, sprayed blue precious nutrient solution and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution every 3 days respectively.
2. the method that bletilla protocorm according to claim 1 is live, it is characterized in that: described bletilla medium is in MS medium, add 6-benzyl aminoadenine, methyl α-naphthyl acetate, bananas juice, use distilled water constant volume, then NaOH aqueous solution adjust ph to 5.8 ~ 6.0 of 0.1mol/L are used, 121 DEG C of sterilizings 20 minutes, be mixed with bletilla medium, wherein said bananas juice 100g banana is added 100mL water in juice extractor beat obtained, in bletilla medium, the concentration of 6-benzyl aminoadenine is 1.5 ~ 2.5mg/L, banana concentration is 75 ~ 120g/L, concentration of NAA is 0.3 ~ 0.8mg/L.
3. the method that bletilla protocorm according to claim 1 is live, is characterized in that: in described step (3), by German Klasmann raw fiber peat soil TS1, coconut palm chaff, tpn according to volume ratio be 200:200:1 mixing, be mixed with matrix.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993935A (en) * | 2016-04-28 | 2016-10-12 | 陕西师范大学 | Rhizome bletillae tissue culture medium, preparation method and application |
| CN107046851A (en) * | 2017-04-12 | 2017-08-18 | 西南林业大学 | Bletilla greenhouse direct sowing and seedling method by material of seed |
| CN107318653A (en) * | 2017-07-28 | 2017-11-07 | 成都大学 | A kind of method for inducing and cultivating of bletilla embryoid |
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
| CN108029555A (en) * | 2017-12-07 | 2018-05-15 | 湖南省农业环境生态研究所 | A kind of bletilla striata method for culturing seedlings |
| CN108739184A (en) * | 2018-06-13 | 2018-11-06 | 遵义湄潭天禾生物科技有限公司 | A kind of saddle-shape bletilla striata tissue culture seedling stem acclimation method |
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246695A (en) * | 2011-05-10 | 2011-11-23 | 云南植物药业有限公司 | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102907323A (en) * | 2012-11-01 | 2013-02-06 | 建始县颐泰生物科技有限公司 | Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103314855A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Bletilla seed tissue culture propagation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
-
2015
- 2015-12-24 CN CN201510989975.6A patent/CN105493861B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246695A (en) * | 2011-05-10 | 2011-11-23 | 云南植物药业有限公司 | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102907323A (en) * | 2012-11-01 | 2013-02-06 | 建始县颐泰生物科技有限公司 | Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103314855A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Bletilla seed tissue culture propagation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
Non-Patent Citations (2)
| Title |
|---|
| 崔瑞勤等: "基于组培快繁技术的白及种子萌发和幼苗形态观察", 《江苏农业科学》 * |
| 罗文秀等: "白及快速繁殖的研究概况", 《中国现代中药》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993935A (en) * | 2016-04-28 | 2016-10-12 | 陕西师范大学 | Rhizome bletillae tissue culture medium, preparation method and application |
| CN105993935B (en) * | 2016-04-28 | 2018-05-22 | 陕西师范大学 | A kind of bletilla tissue culture medium (TCM), preparation method and application |
| CN107046851A (en) * | 2017-04-12 | 2017-08-18 | 西南林业大学 | Bletilla greenhouse direct sowing and seedling method by material of seed |
| CN107046851B (en) * | 2017-04-12 | 2020-07-10 | 西南林业大学 | Bletilla striata greenhouse direct seeding seedling method taking seeds as materials |
| CN107318653A (en) * | 2017-07-28 | 2017-11-07 | 成都大学 | A kind of method for inducing and cultivating of bletilla embryoid |
| CN107318653B (en) * | 2017-07-28 | 2019-01-01 | 成都大学 | A kind of method for inducing and cultivating of bletilla embryoid |
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
| CN108029555A (en) * | 2017-12-07 | 2018-05-15 | 湖南省农业环境生态研究所 | A kind of bletilla striata method for culturing seedlings |
| CN108739184A (en) * | 2018-06-13 | 2018-11-06 | 遵义湄潭天禾生物科技有限公司 | A kind of saddle-shape bletilla striata tissue culture seedling stem acclimation method |
| CN108739184B (en) * | 2018-06-13 | 2020-09-01 | 贵州省农作物品种资源研究所(贵州现代中药材研究所) | Domestication method for tissue culture seed stems of bletilla striata |
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
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