CN105613234B - A kind of method of chrysanthemum bletilla protocorm live streaming - Google Patents
A kind of method of chrysanthemum bletilla protocorm live streaming Download PDFInfo
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- CN105613234B CN105613234B CN201510991468.6A CN201510991468A CN105613234B CN 105613234 B CN105613234 B CN 105613234B CN 201510991468 A CN201510991468 A CN 201510991468A CN 105613234 B CN105613234 B CN 105613234B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
The invention discloses a kind of method of chrysanthemum bletilla protocorm live streaming, chrysanthemum bletilla seed is first grown to serve as protocorm by this method in condition of tissue culture, then direct direct sowing and seedling on the seedbed by the protocorm after tissue culture in greenhouse.The method of the present invention is simple and practicable; first make seed quick-speed germination under condition of tissue culture; later stage is when protocorm needs to transfer culture; it is transplanted in nature; both germination percentage is improved, and reduces production cost, a large amount of chrysanthemum bletilla seedling can be obtained in a short time; meet large area, scale breeding needs, there is production and double meaning economically.
Description
Technical field
The invention belongs to medicinal plant artificial planting technique fields, and in particular to a kind of protocorm of medicinal plant chrysanthemum bletilla
Mating system is broadcast live in stem.
Background technology
Chrysanthemum bletilla (Bletilla ochracea) is orchid family bletilla category herbaceos perennial, is distributed mainly on China
Broad area on the south the Qinling Mountains, wherein in the majority with the Shangluo City in Shaanxi, Zhashui city and Hanzhong City.In recent years since chrysanthemum bletilla exists
Effect is remarkable in terms of whitening, moisturizing, is gradually concerned by people, and in addition it also has exploitation valence well in terms of chemical industry
Value.
There is 30,000 or so, seed in chrysanthemum bletilla fruit pod, but seed germination rate is low under field conditions (factors) or even does not sprout,
Therefore, it solves chrysanthemum bletilla shortage of resources and also mainly passes through tissue culture mode.Tissue culture technique in relation to chrysanthemum bletilla is ripe, but
It is that it requires the environment such as sterile, constant temperature and humidity, causes it to cultivate cost higher.Raising technology in relation to chrysanthemum bletilla, at present may be used
Success is broadcast live under field conditions (factors) in a manner of by generative propagation, although production cost is reduced, from seed to kind
Growth cycle needed for seedling is longer, generally requires 8~10 months time.
Invention content
Technical problem to be solved by the present invention lies in overcoming the problems, such as that the plantation of existing chrysanthemum bletilla exists, a kind of life is provided
Produce the method that at low cost, germination percentage is high and can obtain a large amount of chrysanthemum bletilla seedlings in a short time.
Technical solution is made of following step used by solving above-mentioned technical problem：
1, chrysanthemum bletilla seed is sprouted
Ripe chrysanthemum bletilla fruit pod is chosen, is disappeared successively with aqua sterilisa, mercuric chloride, the ethanol water that volume fraction is 75%
Then poison peels off fruit pod and takes out chrysanthemum bletilla seed, seed uniformly sowed on the chrysanthemum bletilla culture medium in tissue culture bottle,
Temperature is 25 ± 2 DEG C, the tissue culture room culture 65~70 days of 2000~3000Lx of intensity of illumination, illumination 12h/12h, makes seed
Protocorm is expanded and is sprouted into greening, then tissue culture bottle is moved to hardening treatment 5~7 days on greenhouse seedbed, and protocorm is taken out, and is used
Water rinses the culture medium adhered to above well.
2, seedbed is handled
It is 1~1.5m to select the seedbed of intelligent greenhouse, seedbed height, and with waddy barrier, plastic paper is put down every 2 meters for seedbed
It is layered on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, seedling medium is handled
By German Klasmann crude fibres peat soil TS1, coconut palm chaff, the pine bark powder to ferment, carbendazim according to volume ratio
It is 150~250:150~250:80~150:1 mixing, is configured to seedling medium, and hole is arranged at the seedling medium prepared loading bottom
Hole tray in, be filled at the 2/3 of hole tray depth, flatten compacting, hole tray is placed on seedbed, give seedling medium watering, make nursery
Matrix thoroughly irrigates.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to 1~2cm of line-spacing, 1~2cm of spacing in the rows, gently with tweezers
Folder covers hole tray after having sowed to seedling medium surface with preservative film, and it is 20~25 DEG C, relative humidity to control the temperature in greenhouse
It is 40%~60%, and keeps ventilation in greenhouse, sowing was watered every 3~5 days by the gap between hole tray and hole tray after a week
Once.
5, top dressing management
When chrysanthemum bletilla seedling grows two panels true leaf, throw off preservative film, week about respectively the blue precious nutrient solution of sprinkling and
Each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution.
Above-mentioned chrysanthemum bletilla culture medium is that 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice are added in MS culture mediums, is used
Distilled water constant volume, then use 0.1mol/L NaOH aqueous solutions adjust pH value to 5.8~6.0,121 DEG C sterilize 20 minutes, prepare
At chrysanthemum bletilla culture medium, wherein the bananas juice is to add 100mL water to beat obtained, chrysanthemum in juice extractor in 50g bananas
A concentration of 40~60g/L of a concentration of 1.5~2.5mg/L, bananas juice, the methyl α-naphthyl acetate of 6- benzyls aminoadenine are dense in bletilla culture medium
Degree is 0.3~0.8mg/L.
In above-mentioned steps 3, preferably by German Klasmann crude fibres peat soil TS1, coconut palm chaff, the pine bark powder to ferment,
Carbendazim is 200 according to volume ratio:200:100:1 mixing, is configured to seedling medium.
Chrysanthemum bletilla condition of tissue culture is highly developed, 70 days or so in seed sows tissue culture bottle, seed can sprout to
Protocorm, the root and bud of part have occurred, and the present invention is after this stage is by by protocorm domestication, in conjunction with ripe greenhouse
Live streaming system carries out protocorm live streaming, had both improved the germination percentage of chrysanthemum bletilla seed, and reduce production cost, can be
A large amount of chrysanthemum bletilla seedling is obtained in short time, meet large area, scale breeding needs, have production with economically
Double meaning.
Description of the drawings
Fig. 1 be in embodiment 1 chrysanthemum bletilla protocorm sow 8 months after seedling photo.
Fig. 2 be in embodiment 2 chrysanthemum bletilla protocorm sow 8 months after seedling photo.
Fig. 3 be in embodiment 3 chrysanthemum bletilla protocorm sow 8 months after seedling photo.
Specific implementation mode
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments.
Embodiment 1
1, chrysanthemum bletilla seed is sprouted
Ripe chrysanthemum bletilla fruit pod is chosen, is disappeared successively with aqua sterilisa, mercuric chloride, the ethanol water that volume fraction is 75%
Then poison peels off fruit pod and takes out chrysanthemum bletilla seed, seed uniformly sowed on the chrysanthemum bletilla culture medium in tissue culture bottle,
Temperature is 25 ± 2 DEG C, the tissue culture room culture 70 days of intensity of illumination 2500Lx, illumination 12h/12h, and seed greening is made to expand simultaneously
Protocorm is sprouted into, root and bud are will produce on the protocorm of part, then tissue culture bottle is moved into hardening treatment 7 days on greenhouse seedbed, gently
Gently protocorm is taken out, is rinsed well the culture medium adhered to above with water.Wherein chrysanthemum bletilla culture medium is in MS culture mediums
Then middle addition 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice use the NaOH aqueous solution tune of 0.1mol/L with distilled water constant volume
Save pH value to 5.8~6.0,121 DEG C sterilize 20 minutes, be configured to chrysanthemum bletilla culture medium, the bananas juice is by 50g bananas
In juice extractor plus 100mL water beats obtained, a concentration of 2.0mg/L, the perfume (or spice) of 6- benzyl aminoadenines in chrysanthemum bletilla culture medium
Any of several broadleaf plants juice concentration is 50g/L, concentration of NAA 0.5mg/L.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1.5m, seedbed every 2 meters with width be 6cm rectangular waddy every
Gear, plastic paper is laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, seedling medium is handled
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hair
Pine bark powder that ferment is crossed, carbendazim are 200 according to volume ratio:200:100:1 mixing, is configured to seedling medium, is educated what is prepared
Seedling matrix is fitted into the porose hole tray in bottom, is filled at the 2/3 of hole tray depth, flattens compacting, hole tray is placed on seedbed, to educating
Seedling matrix is watered, and seedling medium is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 1cm, spacing in the rows 1cm, gently pressed from both sides with tweezers to educating
Seedling stromal surface, after having sowed with preservative film cover hole tray, control greenhouse in temperature be 20~25 DEG C, relative humidity 40%
~60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between hole tray and hole tray primary after a week.
5, top dressing management
After sowing 1 month, chrysanthemum bletilla seedling grows full two panels true leaf, and emergence rate throws off preservative film 95% or more, every
One week blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, chrysanthemum bletilla plant grows 3~
4 leaves, plant height have reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm.As seen from Figure 1, it broadcasts
After kinds 8 months, the chrysanthemum bletilla plant height in the sprout tumble phase generally more than 12cm, bulb diameter can generally reach 2cm with
On.
Embodiment 2
1, chrysanthemum bletilla seed is sprouted
Ripe chrysanthemum bletilla fruit pod is chosen, is disappeared successively with aqua sterilisa, mercuric chloride, the ethanol water that volume fraction is 75%
Then poison peels off fruit pod and takes out chrysanthemum bletilla seed, seed uniformly sowed on the chrysanthemum bletilla culture medium in tissue culture bottle,
Temperature is 25 ± 2 DEG C, the tissue culture room culture 70 days of intensity of illumination 2000Lx, illumination 12h/12h, and seed greening is made to expand simultaneously
Protocorm is sprouted into, root and bud are will produce on the protocorm of part, then tissue culture bottle is moved into hardening treatment 7 days on greenhouse seedbed, gently
Gently protocorm is taken out, is rinsed well the culture medium adhered to above with water.Wherein chrysanthemum bletilla culture medium is in MS culture mediums
Then middle addition 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice use the NaOH aqueous solution tune of 0.1mol/L with distilled water constant volume
Save pH value to 5.8~6.0,121 DEG C sterilize 20 minutes, be configured to bletilla culture medium, the bananas juice is to squeeze 50g bananas
In juice machine plus 100mL water beats obtained, a concentration of 1.5mg/L of 6- benzyls aminoadenine, banana juice concentration in bletilla culture medium
For 60g/L, concentration of NAA 0.8mg/L.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1.5m, seedbed every 2 meters with width be 6cm rectangular waddy every
Gear, plastic paper is laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, seedling medium is handled
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hair
Pine bark powder that ferment is crossed, carbendazim are 150 according to volume ratio:250:150:1 mixing, is configured to seedling medium, is educated what is prepared
Seedling matrix is fitted into the porose hole tray in bottom, is filled at the 2/3 of hole tray depth, flattens compacting, hole tray is placed on seedbed, to educating
Seedling matrix is watered, and seedling medium is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 1cm, spacing in the rows 1cm, gently pressed from both sides with tweezers to educating
Seedling stromal surface, after having sowed with preservative film cover hole tray, control greenhouse in temperature be 20~25 DEG C, relative humidity 40%
~60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between hole tray and hole tray primary after a week.
5, top dressing management
After sowing 1 month, chrysanthemum bletilla seedling grows full two panels true leaf, and emergence rate throws off preservative film 90% or more, every
One week blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, chrysanthemum bletilla plant grows 3~
4 leaves, plant height have reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm.From Figure 2 it can be seen that broadcasting
After kinds 8 months, the chrysanthemum bletilla plant height that is partially in the sprout tumble phase is more than 8cm, bulb diameter can generally reach 2cm with
On.
Embodiment 3
1, chrysanthemum bletilla seed is sprouted
Ripe chrysanthemum bletilla fruit pod is chosen, is disappeared successively with aqua sterilisa, mercuric chloride, the ethanol water that volume fraction is 75%
Then poison peels off fruit pod and takes out chrysanthemum bletilla seed, seed uniformly sowed on the chrysanthemum bletilla culture medium in tissue culture bottle,
Temperature is 25 ± 2 DEG C, the tissue culture room culture 70 days of intensity of illumination 3000Lx, illumination 12h/12h, and seed greening is made to expand simultaneously
Protocorm is sprouted into, root and bud are will produce on the protocorm of part, then tissue culture bottle is moved into hardening treatment 7 days on greenhouse seedbed, gently
Gently protocorm is taken out, is rinsed well the culture medium adhered to above with water.Wherein chrysanthemum bletilla culture medium is in MS culture mediums
Then middle addition 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice use the NaOH aqueous solution tune of 0.1mol/L with distilled water constant volume
It saves pH value and sterilizes 20 minutes to 5.8~6.0,121 DEG C, bletilla culture medium is configured to, wherein the bananas juice is by 50g bananas
In juice extractor plus 100mL water beats obtained, a concentration of 2.5mg/L, the bananas juice of 6- benzyls aminoadenine in bletilla culture medium
A concentration of 40g/L, concentration of NAA 0.3mg/L.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1.5m, seedbed every 2 meters with width be 6cm rectangular waddy every
Gear, plastic paper is laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, seedling medium is handled
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hair
Pine bark powder that ferment is crossed, carbendazim are 250 according to volume ratio:150:80:1 mixing, is configured to seedling medium, the nursery that will be prepared
Matrix is fitted into the porose hole tray in bottom, is filled at the 2/3 of hole tray depth, flattens compacting, hole tray is placed on seedbed, gives nursery
Matrix is watered, and seedling medium is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 1cm, spacing in the rows 1cm, gently pressed from both sides with tweezers to educating
Seedling stromal surface, after having sowed with preservative film cover hole tray, control greenhouse in temperature be 20~25 DEG C, relative humidity 40%
~60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between hole tray and hole tray primary after a week.
5, top dressing management
After sowing 1 month, chrysanthemum bletilla seedling grows full two panels true leaf, and emergence rate throws off preservative film 75% or more, every
One week blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, chrysanthemum bletilla plant grows 3~
4 leaves, plant height have reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm.As seen from Figure 1, it broadcasts
After kinds 8 months, the chrysanthemum bletilla plant height for being partially in the sprout tumble phase reaches 7cm, part bulb diameter can reach 2cm with
On.
Claims (3)
1. a kind of method of chrysanthemum bletilla protocorm live streaming, it is characterised in that it is made of following step：
(1) chrysanthemum bletilla seed is sprouted
Ripe chrysanthemum bletilla fruit pod is chosen, aqua sterilisa, mercuric chloride, the ethanol water that volume fraction is 75% is used to sterilize successively,
Then it peels off fruit pod and takes out chrysanthemum bletilla seed, seed is uniformly sowed on the chrysanthemum bletilla culture medium in tissue culture bottle, in temperature
Degree is 25 ± 2 DEG C, the tissue culture room culture 65~70 days of 2000~3000Lx of intensity of illumination, illumination 12h/12h, and seed is made to become
It is green to expand and sprout into protocorm, then tissue culture bottle is moved into hardening treatment 5~7 days on greenhouse seedbed, protocorm is taken out, water is used
The culture medium adhered to above is rinsed well；
(2) seedbed is handled
It is 1~1.5m to select the seedbed of intelligent greenhouse, seedbed height, and with waddy barrier, plastic paper is laid in every 2 meters for seedbed
On seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed；
(3) seedling medium is handled
By German Klasmann crude fibres peat soil TS1, coconut palm chaff, the pine bark powder to ferment, carbendazim according to volume ratio be 150
~250:150~250:80~150:1 mixing, is configured to seedling medium, and the seedling medium prepared is packed into the porose cave in bottom
It in disk, is filled at the 2/3 of hole tray depth, flattens compacting, hole tray is placed on seedbed, water to seedling medium, make seedling medium
Thoroughly irrigate；
(4) protocorm is sowed
Using the protocorm after step (1) hardening treatment as kind, according to 1~2cm of line-spacing, 1~2cm of spacing in the rows, gently pressed from both sides with tweezers
To seedling medium surface, hole tray is covered with preservative film after having sowed, the temperature in control greenhouse is 20~25 DEG C, relative humidity is
40%~60%, and ventilation in greenhouse is kept, sowing is after a week every 3~5 days by the gap watering one between hole tray and hole tray
It is secondary；
(5) top dressing management
When chrysanthemum bletilla seedling grows two panels true leaf, preservative film is thrown off, sprays blue precious nutrient solution and phosphoric acid respectively week about
Each 1 time of two amine aqueous solutions.
2. the method for chrysanthemum bletilla protocorm live streaming according to claim 1, it is characterised in that：The chrysanthemum bletilla training
Foster base is to add 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice in MS culture mediums then to use 0.1mol/ with distilled water constant volume
The NaOH aqueous solutions of L adjust pH value and sterilize 20 minutes to 5.8~6.0,121 DEG C, chrysanthemum bletilla culture medium are configured to, wherein described
Bananas juice be by 50g bananas in juice extractor plus 100mL water beat obtained, 6- benzyl aminoadenines in chrysanthemum bletilla culture medium
A concentration of 1.5~2.5mg/L, a concentration of 40~60g/L of bananas juice, concentration of NAA be 0.3~0.8mg/L.
3. the method for chrysanthemum bletilla protocorm live streaming according to claim 1, it is characterised in that：It, will in the step (3)
German Klasmann crude fibres peat soil TS1, coconut palm chaff, the pine bark powder to ferment, carbendazim are 200 according to volume ratio:200:
100:1 mixing, is configured to seedling medium.
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| CN107046851B (en) * | 2017-04-12 | 2020-07-10 | 西南林业大学 | Bletilla striata greenhouse direct seeding seedling method taking seeds as materials |
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
| CN108124732A (en) * | 2017-12-01 | 2018-06-08 | 亳州学院 | Dabie Mountain wild bletilla striata nutrition sowing media configuration method |
| CN108739184B (en) * | 2018-06-13 | 2020-09-01 | 贵州省农作物品种资源研究所(贵州现代中药材研究所) | Domestication method for tissue culture seed stems of bletilla striata |
| CN109006292A (en) * | 2018-08-03 | 2018-12-18 | 遵义市龙驰生物科技有限公司 | A kind of quick seeding growing seedlings method of bletilla striata seeds simple plastic greenhouse |
| CN113951118B (en) * | 2021-10-18 | 2023-02-17 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
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| CN102283115B (en) * | 2011-06-28 | 2013-07-24 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN103348913B (en) * | 2013-06-28 | 2015-04-15 | 江苏茅山地道中药材种植有限公司 | Rapid propagation method for bletilla striata |
| CN103404341B (en) * | 2013-08-05 | 2015-03-04 | 陕西师范大学 | Direct seeding propagation method for bletilla striata in greenhouses |
| CN103404266B (en) * | 2013-08-05 | 2014-07-23 | 陕西师范大学 | Propagation method of bletilla striata |
| CN103766115B (en) * | 2014-01-07 | 2015-05-20 | 陕西师范大学 | Method for transplanting bletilla striata seedlings |
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Inventor after: Cao Xiaoyan Inventor after: Zhang Hui Inventor after: Wang Zhezhi Inventor after: Niu Junfeng Inventor after: Xiao Yaping Inventor after: Wang Shangping Inventor after: Wu Lingling Inventor before: Wang Zhezhi Inventor before: Zhang Hui Inventor before: Niu Junfeng Inventor before: Xiao Yaping Inventor before: Wang Shangping Inventor before: Wu Lingling |
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