CN105493861B - A kind of method of bletilla protocorm live streaming - Google Patents
A kind of method of bletilla protocorm live streaming Download PDFInfo
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- CN105493861B CN105493861B CN201510989975.6A CN201510989975A CN105493861B CN 105493861 B CN105493861 B CN 105493861B CN 201510989975 A CN201510989975 A CN 201510989975A CN 105493861 B CN105493861 B CN 105493861B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of method of bletilla protocorm live streaming, bletilla seed is first grown to serve as protocorm by this method under condition of tissue culture, then direct direct sowing and seedling on the seedbed by the protocorm after tissue culture in greenhouse.The method of the present invention is simple and practicable; first make seed quick-speed germination under condition of tissue culture; later stage is when protocorm needs to transfer culture; it is transplanted in nature; both germination percentage is improved, and reduces production cost, a large amount of bletilla seedling can be obtained in a short time; meet large area, scale bletilla nursery needs, there is production and double meaning economically.
Description
Technical field
The invention belongs to medicinal plant artificial planting technique fields, and in particular to a kind of national secondary endangered medicinal plant is white
And protocorm be broadcast live mating system.
Background technology
Bletilla (Bletilla striata) is orchid family bletilla category herbaceos perennial, main for national 2 grades of endangered plants
It is distributed in the areas such as Guizhou, Hubei, the Southern Shaanxi on the south China Qinling Mountains.《Chinese Plants will》On, bletilla platymiscium is shared
Four kinds, respectively magnificent bletilla, small bletilla, bletilla and chrysanthemum bletilla.《2015 editions pharmacopeia》Regulation bletilla is used as medicine with dry tuber,
There is the benefits of astringing to arrest bleeding, nourishing generate fluid, myogenic pain relieving.It is swollen to be clinically widely used in treatment hemoptysis, haematemesis, traumatic hemorrhage, sore
The illnesss such as poison, pulmonary tuberculosis hemoptysis, ulcer haemorrhage.
There are seed 3~50,000 in bletilla fruit pod, germination rate is low under field conditions (factors) for seed, therefore, solves bletilla resource
Shortage also mainly passes through tissue culture mode.Tissue culture technique is ripe, but its but there is environmental requirements spy stringent, of high cost
Point.By related art method success is broadcast live at present in bletilla by the research group where inventor under field conditions (factors), though
So greatly reduce production cost, but from seed to seedling needed for growth cycle it is longer, generally require 10 months or more
Time.
Invention content
Technical problem to be solved by the present invention lies in overcoming the problems, such as that the plantation of existing bletilla exists, one kind is provided and is produced into
The method that this is low, germination percentage is high and can obtain a large amount of bletilla seedlings in a short time.
Technical solution is made of following step used by solving above-mentioned technical problem：
1, bletilla seed is sprouted
Bletilla seed is sowed on the bletilla culture medium in tissue culture bottle, temperature be 25 ± 2 DEG C, intensity of illumination 2000~
The tissue culture room culture 65~70 days of 3000Lx, illumination 12h/12h make seed sprout into protocorm, then move tissue culture bottle
Hardening treatment one week, protocorm is taken out on to greenhouse seedbed, with water that the medium treatment adhered to above is clean.
2, seedbed is handled
It is 1~1.5m to select the seedbed of intelligent greenhouse, seedbed height, and with waddy baffle, plastic paper is put down every 2 meters for seedbed
It is layered on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, matrix treatments
By German Klasmann crude fibres peat soil TS1, coconut palm chaff, Bravo according to volume ratio be 150~250:150~
250:1 mixing, is configured to matrix, and the matrix prepared is fitted into the porose square plate in bottom, is filled at the 2/3 of square plate depth, pressure
Concora crush is real, and square plate is placed on seedbed, waters to matrix, matrix is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to 2~3cm of line-spacing, 1~2cm of spacing in the rows, gently with tweezers
Folder covers square plate, the temperature in control greenhouse is 20~25 DEG C, relative humidity is after having sowed to stromal surface with preservative film
40%~60%, and ventilation in greenhouse is kept, sowing is after a week every 3~5 days by the gap watering one between square plate and square plate
It is secondary.
5, top dressing management
It waits for that bletilla seedling grows two panels true leaf, throws off preservative film, sprayed blue precious nutrient solution and phosphoric acid diamines respectively every 3 days
Each 1 time of aqueous solution.
Above-mentioned bletilla culture medium is that 6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice are added in MS culture mediums, with distillation
Water constant volume, then use 0.1mol/L NaOH aqueous solutions adjust pH value to 5.8~6.0,121 DEG C sterilize 20 minutes, be configured to white
And culture medium, wherein the bananas juice be by 100g bananas in juice extractor plus 100mL water beat it is obtained, in bletilla culture medium
A concentration of 75~120g/L of a concentration of 1.5~2.5mg/L, banana of 6- benzyl aminoadenines, concentration of NAA be 0.3~
0.8mg/L。
In above-mentioned steps 3, preferably it is according to volume ratio by German Klasmann crude fibres peat soil TS1, coconut palm chaff, Bravo
200:200:1 mixing, is configured to matrix.
Bletilla condition of tissue culture is highly developed, and 70 days or so in seed sows tissue culture bottle, seed can be sprouted to protocorm
Stem, the root and bud of part have occurred, and the present invention is broadcast live after this stage is by by protocorm domestication in conjunction with ripe greenhouse
System carries out protocorm live streaming, both improves germination percentage, and reduce production cost, can obtain in a short time a large amount of
Bletilla seedling, meet large area, scale bletilla nursery needs, there is production and double meaning economically.
Description of the drawings
Fig. 1 be in embodiment 1 bletilla protocorm sow 8 months after bletilla seedling photo.
Fig. 2 be in embodiment 2 bletilla protocorm sow 8 months after bletilla seedling photo.
Fig. 3 be in embodiment 3 bletilla protocorm sow 8 months after bletilla seedling photo.
Specific implementation mode
The present invention is described in more detail with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited only to
These embodiments.
Embodiment 1
1, bletilla seed is sprouted
Bletilla seed is sowed on the bletilla culture medium in tissue culture bottle, is 25 ± 2 DEG C, intensity of illumination in temperature
The tissue culture room culture 70 days of 2500Lx, illumination 12h/12h make seed sprout into protocorm, tissue culture bottle are then moved to temperature
Hardening treatment one week on the seedbed of room will produce fibrous root and bud on the protocorm of part, gently take out protocorm, will be above with water
The medium treatment of attachment is clean.Wherein bletilla culture medium is that 6- benzyls aminoadenine, methyl α-naphthyl acetate, perfume (or spice) are added in MS culture mediums
Then any of several broadleaf plants juice uses the NaOH aqueous solutions of 0.1mol/L to adjust pH value to 5.8~6.0,121 DEG C of 20 points of sterilizings with distilled water constant volume
Clock, is configured to bletilla culture medium, and the bananas juice is to add 100mL water to beat obtained, bletilla in juice extractor in 100g bananas
A concentration of 100g/L of a concentration of 2.0mg/L, banana, the concentration of NAA 0.5mg/L of 6- benzyls aminoadenine in culture medium.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1.5m, seedbed every 2 meters with width be 6cm rectangular waddy every
Shelves, plastic paper are laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, matrix treatments
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hundred
Bacterium is clearly 200 according to volume ratio:200:1 mixing, is configured to matrix, and the matrix prepared is fitted into the porose square plate in bottom, dress
To the 2/3 of square plate depth, compacting is flattened, square plate is placed on seedbed, watered to matrix, matrix is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 2cm, spacing in the rows 1cm, gently pressed from both sides to base with tweezers
Matter surface covers square plate after having sowed with preservative film, the temperature for controlling in greenhouse is 20~25 DEG C, relative humidity be 40%~
60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between square plate and square plate primary after a week.
5, top dressing management
After sowing 1 month, bletilla seedling grows full two panels true leaf, and emergence rate threw off preservative film 95% or more, every 3 days
The blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, bletilla plant grows 3~4 leaves,
Plant height has reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm, as seen from Figure 1, sows 8 months
Afterwards, bletilla plant is in the sprout tumble phase, grows 4~5 leaves, and plant height is generally more than 12cm, and bulb diameter generally can be with
Reach 2cm or more.
Embodiment 2
1, bletilla seed is sprouted
Bletilla seed is sowed on the bletilla culture medium in tissue culture bottle, is 25 ± 2 DEG C, intensity of illumination in temperature
The tissue culture room culture 70 days of 3000Lx, illumination 12h/12h make seed sprout into protocorm, tissue culture bottle are then moved to temperature
Hardening treatment one week on the seedbed of room will produce fibrous root and bud on the protocorm of part, gently take out protocorm, will be above with water
The medium treatment of attachment is clean.Wherein bletilla culture medium is that 6- benzyls aminoadenine, methyl α-naphthyl acetate, perfume (or spice) are added in MS culture mediums
Then any of several broadleaf plants juice uses the NaOH aqueous solutions of 0.1mol/L to adjust pH value to 5.8~6.0,121 DEG C of 20 points of sterilizings with distilled water constant volume
Clock, is configured to bletilla culture medium, and the bananas juice is to add 100mL water to beat obtained, bletilla in juice extractor in 100g bananas
A concentration of 120g/L of a concentration of 2.5mg/L, banana, the concentration of NAA 0.3mg/L of 6- benzyls aminoadenine in culture medium.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1m, seedbed every 2 meters be 6cm with width rectangular waddy baffle,
Plastic paper is laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, matrix treatments
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hundred
Bacterium is clearly 250 according to volume ratio:150:1 mixing, is configured to matrix, and the matrix prepared is fitted into the porose square plate in bottom, dress
To the 2/3 of square plate depth, compacting is flattened, square plate is placed on seedbed, watered to matrix, matrix is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 2cm, spacing in the rows 1cm, gently pressed from both sides to base with tweezers
Matter surface covers square plate after having sowed with preservative film, the temperature for controlling in greenhouse is 20~25 DEG C, relative humidity be 40%~
60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between square plate and square plate primary after a week.
5, top dressing management
After sowing 1 month, bletilla seedling grows full two panels true leaf, and emergence rate threw off preservative film 90% or more, every 3 days
The blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, bletilla plant grows 3~4 leaves,
Plant height has reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm.From Figure 2 it can be seen that sowing 8 months
Afterwards, bletilla plant is in the sprout tumble phase, partly grows 4~5 leaves, plant height is generally more than 12cm, and bulb diameter is universal
2cm or more can be reached.
Embodiment 3
1, bletilla seed is sprouted
Bletilla seed is sowed on the bletilla culture medium in tissue culture bottle, is 25 ± 2 DEG C, intensity of illumination in temperature
The tissue culture room culture 70 days of 2000Lx, illumination 12h/12h make seed sprout into protocorm, tissue culture bottle are then moved to temperature
Hardening treatment one week on the seedbed of room will produce fibrous root and bud on the protocorm of part, gently take out protocorm, will be above with water
The medium treatment of attachment is clean.Wherein bletilla culture medium is that 6- benzyls aminoadenine, methyl α-naphthyl acetate, perfume (or spice) are added in MS culture mediums
Then any of several broadleaf plants juice uses the NaOH aqueous solutions of 0.1mol/L to adjust pH value to 5.8~6.0,121 DEG C of 20 points of sterilizings with distilled water constant volume
Clock, is configured to bletilla culture medium, and the bananas juice is to add 100mL water to beat obtained, bletilla in juice extractor in 100g bananas
A concentration of 75g/L of a concentration of 1.5mg/L, banana, the concentration of NAA 0.8mg/L of 6- benzyls aminoadenine in culture medium.
2, seedbed is handled
Select intelligent greenhouse seedbed, seedbed height be 1.5m, seedbed every 2 meters with width be 6cm rectangular waddy every
Shelves, plastic paper are laid on seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed.
3, matrix treatments
By German Klasmann crude fibres peat soil TS1 (being provided by the Xi'an flowers markets Yan Jin rainbow matrix), coconut palm chaff, hundred
Bacterium is clearly 150 according to volume ratio:250:1 mixing, is configured to matrix, and the matrix prepared is fitted into the porose square plate in bottom, dress
To the 2/3 of square plate depth, compacting is flattened, square plate is placed on seedbed, watered to matrix, matrix is made thoroughly to irrigate.
4, protocorm is sowed
Using the protocorm after step 1 hardening treatment as kind, according to line-spacing 2cm, spacing in the rows 1cm, gently pressed from both sides to base with tweezers
Matter surface covers square plate after having sowed with preservative film, the temperature for controlling in greenhouse is 20~25 DEG C, relative humidity be 40%~
60%, and ventilation in greenhouse is kept, sowing was watered every 5 days by the gap between square plate and square plate primary after a week.
5, top dressing management
After sowing 1 month, bletilla seedling grows full two panels true leaf, and emergence rate threw off preservative film 85% or more, every 3 days
The blue precious nutrient solution of sprinkling and each 1 time of di(2-ethylhexyl)phosphate amine aqueous solution respectively.After sowing 5 months, bletilla plant grows 3~4 leaves,
Plant height has reached the standard cultivated under condition of tissue culture 8~10 months generally more than 6cm.As seen from Figure 3, it sows 8 months
Afterwards, bletilla plant is in the sprout tumble phase, and most plant grow 4~5 leaves, and plant height is commonly 10~12cm, and ball
Stem diameter majority can reach 2cm or more.
Claims (3)
1. a kind of method of bletilla protocorm live streaming, it is characterised in that it is made of following step：
(1) bletilla seed is sprouted
Bletilla seed is sowed on the bletilla culture medium in tissue culture bottle, temperature be 25 ± 2 DEG C, intensity of illumination 2000~
The tissue culture room culture 65~70 days of 3000Lx, illumination 12h/12h make seed sprout into protocorm, then move tissue culture bottle
Hardening treatment one week, protocorm is taken out on to greenhouse seedbed, with water that the medium treatment adhered to above is clean；
(2) seedbed is handled
It is 1~1.5m to select the seedbed of intelligent greenhouse, seedbed height, and with waddy barrier, plastic paper is tiled every 2 meters for seedbed
On seedbed, the surrounding of plastic paper is fixed on seedbed edge, it is ensured that the water of 2cm high can be kept on seedbed；
(3) matrix treatments
By German Klasmann crude fibres peat soil TS1, coconut palm chaff, Bravo according to volume ratio be 150~250:150~250:1
Mixing is configured to matrix, and the matrix prepared is fitted into the porose square plate in bottom, is filled at the 2/3 of square plate depth, flattens pressure
It is real, square plate is placed on seedbed, waters to matrix, matrix is made thoroughly to irrigate；
(4) protocorm is sowed
Using the protocorm after step (1) hardening treatment as kind, according to 2~3cm of line-spacing, 1~2cm of spacing in the rows, gently pressed from both sides with tweezers
To stromal surface, after having sowed with preservative film cover square plate, control greenhouse in temperature be 20~25 DEG C, relative humidity 40%
~60%, and ventilation in greenhouse is kept, sowing was watered every 3~5 days by the gap between square plate and square plate primary after a week；
(5) top dressing management
It waits for that bletilla seedling grows two panels true leaf, throws off preservative film, the blue precious nutrient solution of sprinkling and di(2-ethylhexyl)phosphate aqueous amine were molten respectively every 3 days
Each 1 time of liquid.
2. the method for bletilla protocorm live streaming according to claim 1, it is characterised in that：The bletilla culture medium be
6- benzyls aminoadenine, methyl α-naphthyl acetate, bananas juice are added in MS culture mediums, with distilled water constant volume, then use the NaOH of 0.1mol/L
Aqueous solution adjusts pH value and sterilizes 20 minutes to 5.8~6.0,121 DEG C, bletilla culture medium is configured to, wherein the bananas juice is
By 100g bananas in juice extractor plus 100mL water beat it is obtained, a concentration of the 1.5 of 6- benzyls aminoadenine in bletilla culture medium~
A concentration of 75~120g/L of 2.5mg/L, banana, concentration of NAA are 0.3~0.8mg/L.
3. the method for bletilla protocorm live streaming according to claim 1, it is characterised in that：It, will be German in the step (3)
Klasmann crude fibre peat soils TS1, coconut palm chaff, Bravo are 200 according to volume ratio:200:1 mixing, is configured to matrix.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993935B (en) * | 2016-04-28 | 2018-05-22 | 陕西师范大学 | A kind of bletilla tissue culture medium (TCM), preparation method and application |
| CN107046851B (en) * | 2017-04-12 | 2020-07-10 | 西南林业大学 | Bletilla striata greenhouse direct seeding seedling method taking seeds as materials |
| CN107318653B (en) * | 2017-07-28 | 2019-01-01 | 成都大学 | A kind of method for inducing and cultivating of bletilla embryoid |
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
| CN108029555B (en) * | 2017-12-07 | 2019-11-29 | 湖南省农业环境生态研究所 | A kind of bletilla striata method for culturing seedlings |
| CN108739184B (en) * | 2018-06-13 | 2020-09-01 | 贵州省农作物品种资源研究所(贵州现代中药材研究所) | Domestication method for tissue culture seed stems of bletilla striata |
| CN108770668A (en) * | 2018-06-22 | 2018-11-09 | 遵义市播州区惠联生态农业发展有限公司 | A kind of purple dendrobium capsule seed seedling-raising technique |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246695A (en) * | 2011-05-10 | 2011-11-23 | 云南植物药业有限公司 | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102907323A (en) * | 2012-11-01 | 2013-02-06 | 建始县颐泰生物科技有限公司 | Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103314855A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Bletilla seed tissue culture propagation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
-
2015
- 2015-12-24 CN CN201510989975.6A patent/CN105493861B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102246695A (en) * | 2011-05-10 | 2011-11-23 | 云南植物药业有限公司 | Method for preparing common bletilla pseudobulb in culture vessel and special culture media thereof |
| CN102283115A (en) * | 2011-06-28 | 2011-12-21 | 陕西科技大学 | Method for quickly reproducing bletilla striata seedlings |
| CN102907323A (en) * | 2012-11-01 | 2013-02-06 | 建始县颐泰生物科技有限公司 | Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds |
| CN102972290A (en) * | 2012-11-15 | 2013-03-20 | 贵州师范大学 | Method for improving transplanting survival rate of rhizoma bletillae tissue culture seedling |
| CN103314855A (en) * | 2013-07-08 | 2013-09-25 | 重庆市秀山红星中药材开发有限公司 | Bletilla seed tissue culture propagation method |
| CN103404341A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Direct seeding propagation method for Bletilla Striata in greenhouses |
Non-Patent Citations (2)
| Title |
|---|
| 基于组培快繁技术的白及种子萌发和幼苗形态观察;崔瑞勤等;《江苏农业科学》;20150225;第43卷(第2期);第238-240页 * |
| 白及快速繁殖的研究概况;罗文秀等;《中国现代中药》;20071116;第9卷(第11期);第36-38页 * |
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