CN107646591A - A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix - Google Patents
A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix Download PDFInfo
- Publication number
- CN107646591A CN107646591A CN201710917429.0A CN201710917429A CN107646591A CN 107646591 A CN107646591 A CN 107646591A CN 201710917429 A CN201710917429 A CN 201710917429A CN 107646591 A CN107646591 A CN 107646591A
- Authority
- CN
- China
- Prior art keywords
- bletilla striata
- parts
- protocorm
- seedling
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
A kind of method cultivated the invention discloses bletilla striata direct sowing and seedling matrix and using the matrix, the matrix include 1~5 part of 20~50 parts of rice husk, 40~50 parts of peat soil, 20~30 parts of perlite, 30~40 parts of thin river sand and Mancozeb；Breeding method includes sowing bletilla striata seeds in the medium, cultivated under conditions of 23~26 DEG C, 1000~2000Lx of illumination, 8~12h/d of illumination, sprout protocorm, bletilla striata direct sowing and seedling matrix then is arrived into protocorm plantation, grown in 25~30 DEG C, relative humidity under the conditions of 50~70%, and carry out water and fertilizer management.The remarkable advantage of the present invention can effectively improve the planting percent of the live production seedling of protocorm for the bletilla striata direct sowing and seedling matrix, simultaneously, the present invention tissue culture and it is naturally live be combined by way of breed seedling, significantly reduce production cost, shorten the seeling industry cycle.
Description
Technical field
Field is cultivated the invention belongs to the bletilla striata, more particularly to a kind of bletilla striata direct sowing and seedling matrix and is trained using the matrix
The method educated.
Background technology
The bletilla striata (scientific name：Bletillastriata it is) herbaceos perennial, dry tuber is used as medicine, and has astringing to arrest bleeding,
Detumescence and promoting granulation and other effects.The bletilla striata has extensive medical value and Ornamental value.It is mainly used in astringing to arrest bleeding, detumescence and promoting granulation
Deng, flower has the colors such as purplish red, white, blue, yellow and powder, can potted plant indoor appreciation, can also intersperse in more cover flower stand, flower border or front yard
One jiao of institute.
The seed of the bletilla striata is superfine small, seemingly powdered, without endosperm, the embryo of the ateliosis of only several cellularities.
It is white loft at present substantially by the processed rear hyoscine of collection natural crude drugs.Due to the increase of demand, cause medicinal herb grower to wild
Resource is largely excavated, and wild resource is constantly being reduced, and bletilla striata seeds are tiny, germination rate is extremely low, natural propagation is difficult and grow
Slowly, wild bletilla striata is drastically reduced, endangered.
Bletilla striata shortage of resources is solved at present mainly by way of tissue cultures, but because tissue cultures require sterile, permanent
The environment such as temperature, constant humidity, growth cycle is also longer needed for from seed to seedling, generally requires the time of more than 8 months, causes its kind
Seedling cultivation cost is higher, and tissue-cultured seedling transplanting survival rate is low.
The content of the invention
Goal of the invention：The first object of the present invention, which is to provide one kind, can allow bletilla striata protocorm to form kind under field conditions (factors)
Seedling, improve the bletilla striata direct sowing and seedling matrix of the planting percent of the live production seedling of bletilla striata protocorm；
The second object of the present invention is to provide the method cultivated using the matrix, and this method effectively shortens seedling
Production cycle, and planting percent is high, is adapted to large-scale production seedling.
Technical scheme：Bletilla striata direct sowing and seedling matrix of the present invention, including the following raw material based on volume parts：Rice husk 20~50
Part, 40~50 parts of peat soil, 20~30 parts of perlite, 30~40 parts of thin river sand and 1~5 part of Mancozeb.
The present invention enables to tissue culture protocorm germination and growth shape in the matrix using the seedling medium of raw material composition
Into seedling.Preferably, 20~30 parts of rice husk, 30~40 parts of peat soil, 20~25 parts of perlite, 30~35 parts of thin river sand and for gloomy
3~5 parts of MnZn.
The method that the present invention is cultivated using bletilla striata direct sowing and seedling matrix, comprises the following steps：
(1) bletilla striata protocorm is cultivated：By bletilla striata seeds sow in the medium, 23~26 DEG C, illumination 1000~
Cultivated under conditions of 2000Lx, 8~12h/d of illumination, sprout protocorm；
Cultivating under this condition can be such that seed aseptically sprouts quickly, form protocorm.
(2) live protocorm：By protocorm plantation arrive bletilla striata direct sowing and seedling matrix, 25~30 DEG C, relative humidity 50~
Grown under the conditions of 70%, and carry out water and fertilizer management.
The present invention uses 25~30 DEG C, the condition of relative humidity 50~70%, is advantageous in the range of this temperature and humidity
Protocorm germination and growth goes out leaf, if temperature is too low protocorm can be caused to sprout slowly, temperature is too high to suppress protocorm sprouting；
And humidity it is too low can cause protocorm dehydration inactivation, humidity is too high, and protocorm can be caused to rot, and influences the planting percent of protocorm.
Furtherly, in step (1) of the present invention, culture medium may include to spend precious No. 1 10~15g/L, methyl α-naphthyl acetate by densimeter
0.2~0.5mg/L, 25~35g/L of sucrose, 50~80g/L of potato, 1~2mg/L of glycine, 0.5~1.0mg/L of nicotinic acid, dimension
Raw plain 0.5~1.0mg/L of B1,5.5~6g/L of 0.5~1.0mg/L of vitamin B6 and agar powder.
The present invention is a large amount of and micro- in the precious No. 1 MS or N6 culture medium replaced used in conventional bletilla striata axenic germination using spending
Secondary element, the culture medium can simplify operation, and cost is lower, and can faster form protocorm.
Further say, in step (1), a diameter of 0.2~0.8cm of protocorm.In step (2), the spacing in the rows of plantation is 2
~3cm, line-spacing are 3~5cm.Water and fertilizer management treats that bletilla striata protocorm is grown after planting to be sprayed water every 2~4 days to the bletilla striata
After two panels cotyledon, thunder power liquid seaweed extract and potassium dihydrogen phosphate were sprayed respectively every 4~7 days.The spraying concentration of liquid seaweed extract is
1500~1800 times.The spraying concentration of potassium dihydrogen phosphate is 2000~2500 times.
The present invention water and fertilizer management be able to ensure that protocorm imputrescibility or inactivation, in addition, add thunder power alga fertilizer and
Potassium dihydrogen phosphate ensure that the nutrition that protocorm is sprouted and growth is required.
Beneficial effect：Compared with prior art, remarkable advantage of the invention is：The bletilla striata direct sowing and seedling matrix can allow white
Splendid achnatherum protocorm forms seedling under field conditions (factors), because seedling is to be formed under field conditions (factors), the adaptability to environment to soil
Greatly improve, the kind transplantation of seedlings planting percent of production is high；Meanwhile the present invention tissue culture and it is naturally live be combined by way of breed
Seedling, production cost is significantly reduced, shorten the seeling industry cycle, and bletilla striata protocorm planting percent can reach 98%
More than, meet the requirement of large-scale production seedling.
Brief description of the drawings
Fig. 1 is gained bletilla striata protocorm microscope figure after bletilla striata seeds of the present invention are sowed 30 days；
Picture after Fig. 2 grows 4 months for bletilla striata protocorm of the present invention is live into matrix.
Embodiment
Below in conjunction with the accompanying drawings and embodiment is described in further details to technical scheme.
Embodiment 1
(1) bletilla striata seeds aseptic seeding obtains protocorm：
Bletilla striata culture medium is configured, specific formula is as follows：Spend treasured No. 1 10g/L, methyl α-naphthyl acetate 0.3mg/L, sucrose 25g/L, Ma Ling
Potato 60g/L, glycine 2mg/L, nicotinic acid 1.0mg/L, vitamin B1 1mg/L, vitamin B6 0.5mg/L, agar powder 5.5g/L.
The preparation method of the culture medium comprises the following steps：Weigh and spend treasured No. 1 10g, methyl α-naphthyl acetate 0.3mg, sucrose 25g, horse
Bell potato 60g, glycine 2mg, nicotinic acid 1.0mg, vitamin B1 1mg, vitamin B6 0.5mg, agar powder 5.5g, adds pure water extremely
1L, mix, regulation pH value to 5.8, the high-temperature sterilization under the conditions of 121 DEG C, wherein, potato is that peeling stirs into pureed.
Ripe bletilla striata capsule is chosen, is successively carried out bletilla striata capsule with 10% sodium hypochlorite, 0.1% mercuric chloride, sterilized water
Disinfect, aseptically cut capsule and take out bletilla striata seeds, seed is uniformly sowed in bletilla striata culture medium, in temperature
26 ± 3 DEG C, illumination 1500Lx, illumination 10h/d culturing room in cultivate 30 days, expand seed greening and sprout into diameter 0.2
~0.8cm protocorm, as shown in figure 1, the protocorm of tissue culture bottle is taken out, the culture medium adhered to above is rinsed with clear water and done
Only.
(2) preparation of direct sowing and seedling matrix：
By 20 parts of rice husks of volume ratio, 40 parts of peat soils, 25 parts of perlites, 35 thin river sands, 5 part of 80% active ingredient Dai Sen
MnZn mixing, which is mixed thoroughly, is configured to seedling medium, and the seedling medium prepared is fitted into bottom has in the hole tray of air-vent, and waters
It is impregnated with matrix, after configuration is good, places it in the shade greenhouse that shading rate is 80%, the planting percent of seedling is up to 94.5%.
(3) protocorm is live：
Using the protocorm obtained by step 1 as original seed, seedling medium surface described in step 2 is transplanted to, according to spacing in the rows 2cm,
Line-spacing 4cm is sowed, and after the completion of sowing, the thick thin river sands of one layer of 0.3cm is renovated on bletilla striata protocorm surface and are used for fixed original
Bulb so that protocorm half reveals in atmosphere, and controls the temperature in greenhouse in 25~30 DEG C, relative humidity 50~70%, and
Keep divulging information in greenhouse.
(4) water and fertilizer management
Water spray moisturizing after planting is carried out to the bletilla striata in hole tray every 2~3 days so that the humidity of seedling medium maintains all the time
Between 60~70%.After bletilla striata protocorm grows two panels cotyledon, start to spray the functional forms of Lei Li 2000 sea respectively every 5 days
Algae solution fertilizer and potassium dihydrogen phosphate are each once, and the functional marine alga liquid fertilizer spraying concentrations of Lei Li 2000 are 1500 times, potassium dihydrogen phosphate
Spraying concentration is 2000 times.After live 4 months, high 5~15cm bletilla striata seedling is produced, as shown in Figure 2.
Embodiment 2
(1) bletilla striata seeds aseptic seeding obtains protocorm：
Bletilla striata culture medium is configured, specific formula is as follows：Spend treasured No. 1 15g/L, methyl α-naphthyl acetate 0.5mg/L, sucrose 30g/L, Ma Ling
Potato 80g/L, glycine 2mg/L, nicotinic acid 1.0mg/L, vitamin B1 0.5mg/L, vitamin B6 1.0mg/L, agar powder 6g/L.
The preparation method of the culture medium comprises the following steps：Weigh and spend treasured No. 1 15g, methyl α-naphthyl acetate 0.5mg, sucrose 30g, horse
Bell potato 80g, glycine 2mg, nicotinic acid 1.0mg, vitamin B1 0.5mg, vitamin B6 1.0mg, agar powder 6g, adds pure water extremely
1L, mix, regulation pH value to 5.8, the high-temperature sterilization under the conditions of 121 DEG C, wherein, potato is that peeling stirs into pureed.
Ripe bletilla striata capsule is chosen, is successively carried out bletilla striata capsule with 10% sodium hypochlorite, 0.1% mercuric chloride, sterilized water
Disinfect, aseptically cut capsule and take out bletilla striata seeds, seed is uniformly sowed in bletilla striata culture medium, in temperature
26 ± 3 DEG C, illumination 1800Lx, illumination 10h/d culturing room in cultivate 40 days, expand seed greening and sprout into diameter 0.2
~0.8cm protocorm, the protocorm of tissue culture bottle is taken out, rinsed well the culture medium adhered to above with clear water.
(2) preparation of direct sowing and seedling matrix：
By 30 parts of rice husks of volume ratio, 35 parts of peat soils, 20 parts of perlites, 30 thin river sands, 4 part of 80% active ingredient Dai Sen
MnZn mixing, which is mixed thoroughly, is configured to seedling medium, and the seedling medium prepared is fitted into bottom has in the hole tray of air-vent, and waters
It is impregnated with matrix, after configuration is good, places it in the shade greenhouse that shading rate is 85%, the planting percent of seedling is up to 97.7%.
(3) protocorm is live：
Using the protocorm obtained by step 1 as original seed, seedling medium surface described in step 2 is transplanted to, according to spacing in the rows 3cm,
Line-spacing 3cm is sowed, and after the completion of sowing, the thick thin river sands of one layer of 0.3cm is renovated on bletilla striata protocorm surface and are used for fixed original
Bulb so that protocorm half reveals in atmosphere, and controls temperature in greenhouse in 25~30 DEG C, relative humidity 50~70%,
And keep divulging information in greenhouse.
(4) water and fertilizer management：
Water spray moisturizing after planting is carried out to the bletilla striata in hole tray every 2~3 days so that the humidity of seedling medium maintains all the time
Between 60~70%.After bletilla striata protocorm grows two panels cotyledon, start to spray the functional forms of Lei Li 2000 sea respectively every 5 days
Algae solution fertilizer and potassium dihydrogen phosphate are each once, and the functional marine alga liquid fertilizer spraying concentrations of Lei Li 2000 are 1800 times, potassium dihydrogen phosphate
Spraying concentration is 2500 times.After live 4 months, high 5~15cm bletilla striata seedling is produced.
Embodiment 3
(1) bletilla striata seeds aseptic seeding obtains protocorm：
Bletilla striata culture medium is configured, specific formula is as follows：Spend treasured No. 1 12g/L, methyl α-naphthyl acetate 0.5mg/L, sucrose 35g/L, Ma Ling
Potato 60g/L, glycine 2mg/L, nicotinic acid 1.0mg/L, vitamin B1 0.5mg/L, vitamin B6 1.0mg/L, agar powder 6g/L.
The preparation method of the culture medium comprises the following steps：Weigh and spend treasured No. 1 12g, methyl α-naphthyl acetate 0.5mg, sucrose 35g, horse
Bell potato 60g, glycine 2mg, nicotinic acid 1.0mg, vitamin B1 0.5mg, vitamin B6 1.0mg, agar powder 6g, adds pure water extremely
1L, mix, regulation pH value to 5.8, the high-temperature sterilization under the conditions of 121 DEG C, wherein, potato is that peeling stirs into pureed.
Ripe bletilla striata capsule is chosen, is successively carried out bletilla striata capsule with 10% sodium hypochlorite, 0.1% mercuric chloride, sterilized water
Disinfect, aseptically cut capsule and take out bletilla striata seeds, seed is uniformly sowed in bletilla striata culture medium, in temperature
26 ± 3 DEG C, illumination 2000Lx, illumination 10h/d culturing room in cultivate 45 days, expand seed greening and sprout into diameter 0.2
~0.8cm protocorm, the protocorm of tissue culture bottle is taken out, rinsed well the culture medium adhered to above with clear water.
(2) preparation of direct sowing and seedling matrix：
By 25 parts of rice husks of volume ratio, 35 parts of peat soils, 20 parts of perlites, 35 thin river sands, 3 part of 80% active ingredient Dai Sen
MnZn mixing, which is mixed thoroughly, is configured to seedling medium, and the seedling medium prepared is fitted into bottom has in the hole tray of air-vent, and waters
It is impregnated with matrix, after configuration is good, places it in the shade greenhouse that shading rate is 75%, the planting percent of seedling is up to 98.6%.
(3) protocorm is live：
Using the protocorm obtained by step 1 as original seed, seedling medium surface described in step 2 is transplanted to, according to spacing in the rows 4cm,
Line-spacing 4cm is sowed, and after the completion of sowing, the thick thin river sands of one layer of 0.4cm is renovated on bletilla striata protocorm surface and are used for fixed original
Bulb so that protocorm half reveals in atmosphere, and controls temperature in greenhouse in 25~30 DEG C, relative humidity 50~70%,
And keep divulging information in greenhouse.
(4) water and fertilizer management：
Water spray moisturizing after planting is carried out to the bletilla striata in hole tray every 2~3 days so that the humidity of seedling medium maintains all the time
Between 60~70%.After bletilla striata protocorm grows two panels cotyledon, start to spray the functional forms of Lei Li 2000 sea respectively every 4 days
Algae solution fertilizer and potassium dihydrogen phosphate are each once, and the functional marine alga liquid fertilizer spraying concentrations of Lei Li 2000 are 1800 times, potassium dihydrogen phosphate
Spraying concentration is 2500 times.After live 4 months, high 5~15cm bletilla striata seedling is produced.
Embodiment 4
(1) bletilla striata seeds aseptic seeding obtains protocorm：
Bletilla striata culture medium is configured, specific formula is as follows：Spend treasured No. 1 12g/L, methyl α-naphthyl acetate 0.2mg/L, sucrose 35g/L, Ma Ling
Potato 50g/L, glycine 1mg/L, nicotinic acid 1.0mg/L, vitamin B1 0.5mg/L, vitamin B6 1.0mg/L, agar powder 6g/L.
The preparation method of the culture medium comprises the following steps：Spend treasured No. 1 12g, methyl α-naphthyl acetate 0.2mg, sucrose 35g, potato
50g, glycine 1mg, nicotinic acid 1.0mg, vitamin B1 0.5mg, vitamin B6 1.0mg, agar powder 6g, add pure water to 1L,
Mix, regulation pH value to 5.8, the high-temperature sterilization under the conditions of 121 DEG C, wherein, potato is that peeling stirs into pureed.
Ripe bletilla striata capsule is chosen, is successively carried out bletilla striata capsule with 10% sodium hypochlorite, 0.1% mercuric chloride, sterilized water
Disinfect, aseptically cut capsule and take out bletilla striata seeds, seed is uniformly sowed in bletilla striata culture medium, in temperature
26 ± 3 DEG C, illumination 1000Lx, illumination 8h/d culturing room in cultivate 40 days, expand seed greening and sprout into diameter 0.2~
0.8cm protocorm, the protocorm of tissue culture bottle is taken out, rinsed well the culture medium adhered to above with clear water.
(2) preparation of direct sowing and seedling matrix：
By 50 parts of rice husks of volume ratio, 30 parts of peat soils, 30 parts of perlites, 40 thin river sands, 1 part of 80% active ingredient Dai Sen
MnZn mixing, which is mixed thoroughly, is configured to seedling medium, and the seedling medium prepared is fitted into bottom has in the hole tray of air-vent, and waters
It is impregnated with matrix, after configuration is good, places it in the shade greenhouse that shading rate is 75%, the planting percent of seedling is up to 92.5%.
(3) protocorm is live：
Using the protocorm obtained by step 1 as original seed, seedling medium surface described in step 2 is transplanted to, according to spacing in the rows 4cm,
Line-spacing 5cm is sowed, and after the completion of sowing, the thick thin river sands of one layer of 0.4cm is renovated on bletilla striata protocorm surface and are used for fixed original
Bulb so that protocorm half reveals in atmosphere, and controls temperature in greenhouse in 25~30 DEG C, relative humidity 50~70%,
And keep divulging information in greenhouse.
(4) water and fertilizer management：
Water spray moisturizing after planting is carried out to the bletilla striata in hole tray every 3~4 days so that the humidity of seedling medium maintains all the time
Between 60~70%.After bletilla striata protocorm grows two panels cotyledon, start to spray the functional forms of Lei Li 2000 sea respectively every 7 days
Algae solution fertilizer and potassium dihydrogen phosphate are each once, and the functional marine alga liquid fertilizer spraying concentrations of Lei Li 2000 are 1800 times, potassium dihydrogen phosphate
Spraying concentration is 2500 times.After live 4 months, high 5~15cm bletilla striata seedling is produced.
Embodiment 5
6 groups of parallel tests are designed, basic step is same as Example 1, and difference is the preparation of direct sowing and seedling matrix,
Shown in specific as follows：
1st, 20 parts of rice husks, 50 parts of peat soils, 20 parts of perlites, 40 thin river sands, the Mancozeb of 1 part of 80% active ingredient；
2nd, 20 parts of rice husks, 40 parts of peat soils, 20 parts of perlites, 35 thin river sands, the Mancozeb of 3 part of 80% active ingredient；
3rd, 30 parts of rice husks, 30 parts of peat soils, 25 parts of perlites, 30 thin river sands, the Mancozeb of 5 part of 80% active ingredient；
4th, 50 parts of rice husks, 40 parts of peat soils, 30 parts of perlites, 30 thin river sands, the Mancozeb of 5 part of 80% active ingredient；
5th, 15 parts of rice husks, 55 parts of peat soils, 15 parts of perlites, 45 thin river sands, the maneb of 0.5 part of 80% active ingredient
Zinc；
6th, 55 parts of rice husks, 35 parts of peat soils, 35 parts of perlites, 25 thin river sands, the Mancozeb of 6 part of 80% active ingredient；
Matrix prepared by embodiment 5 carries out cultivation discovery, can be allowed using the raw material components in the 1-4 group scope of the invention
Bletilla striata protocorm forms seedling under field conditions (factors), because seedling is to be formed under field conditions (factors), to adaptation of the environment to soil
Property greatly improve, and then improve the planting percent of kind of transplantation of seedlings, be more than 92.5%, and the planting percent of 2-3 groups is more excellent, reaches
97.7% and 98.6% or so；And the Feedstock Component content of the constituent content, i.e. 5-6 groups outside the scope of the invention is used, its seedling
Rate substantially reduces, and respectively 85.5% and 80.7%, this is due in above-mentioned component, and rice husk primarily serves loose ventilative work
With after band protocorm forms seedling, it can slowly rot to be formed organic matter supply bletilla striata growth of seedling, but rice husk is too in component
It is more, it is easily caused matrix space and increases, the moisture in matrix is easily lost in, so as to cause protocorm or seedling dehydration to influence into
Seedling rate, rice husk component planting percent at 20~30 parts is substantially higher than other in example 5, and the 6th group of experiment is in rice in example 5
When shell component is 55 parts, its planting percent is minimum, and component protocorm dehydration deactivation phenomenom is serious.
In addition, during protocorm is live, peat soil is mainly that protocorm provides organic matter nutrition, peat soil in component
Content is too high to cause matrix hardened, and moisture is high, ventilative poor, easily causes protocorm and rots, it is demonstrated from example
Component control at 30~40 parts be optimal, and in example 5 the 5th group of peat soil component for 55 parts when, its seedling planting percent is relatively low, original
Stem-bulb rot phenomenon is serious；
Because the present invention is to produce protocorm using aseptic seeding, seedling, mother are formed in matrix using protocorm is live
Originally it is to be transformed into collarium border from gnotobasis, therefore addition Mancozeb avoids pathogenic bacterial infection protocorm and causes to rot,
And after protocorm grows root system, root system meeting profitable probliotics invade to form mycorhiza, promote plant to absorb nutrition, Mancozeb
Amount is too small to cause matrix sterilization not thorough, cause protocorm to rot, too big some probiotics that can be killed again in matrix of its amount,
The follow-up growing way of seedling is influenceed, therefore the component of 80% Mancozeb is set in a zone of reasonableness in the present invention, is 1~5
Part, wherein 3~5 parts are optimal.
Claims (9)
1. a kind of bletilla striata direct sowing and seedling matrix, it is characterised in that including the following raw material based on volume parts：20~50 parts of rice husk, mud
1~5 part of 40~50 parts of charcoal soil, 20~30 parts of perlite, 30~40 parts of thin river sand and Mancozeb.
2. bletilla striata direct sowing and seedling matrix according to claim 1, it is characterised in that：20~30 parts of the rice husk, peat soil
3~5 parts of 30~40 parts, 20~25 parts of perlite, 30~35 parts of thin river sand and Mancozeb.
3. the method cultivated using the bletilla striata direct sowing and seedling matrix described in claim 1, it is characterised in that including following step
Suddenly：
(1) bletilla striata protocorm is cultivated：By bletilla striata seeds sow in the medium, 23~26 DEG C, 1000~2000Lx of illumination, light
According to being cultivated under conditions of 8~12h/d, protocorm is sprouted；
(2) live protocorm：Bletilla striata direct sowing and seedling matrix is arrived into protocorm plantation, in 25~30 DEG C, relative humidity 50~70%
Under the conditions of grow, and carry out water and fertilizer management.
4. the method according to claim 3 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：Step
(1) in, the culture medium is included spending No. 1 10~15g/L of treasured, 0.2~0.5mg/L of methyl α-naphthyl acetate, 25~35g/ of sucrose by densimeter
L, 50~80g/L of potato, 1~2mg/L of glycine, 0.5~1.0mg/L of nicotinic acid, 0.5~1.0mg/L of vitamin B1, dimension life
Plain 0.5~1.0mg/L of B6 and 5.5~6g/L of agar powder.
5. the method according to claim 3 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：Step
(1) in, a diameter of 0.2~0.8cm of the protocorm.
6. the method according to claim 3 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：Step
(2) in, the spacing in the rows of the plantation is 2~3cm, and line-spacing is 3~5cm.
7. the method according to claim 3 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：Step
(2) in, the water and fertilizer management treats that bletilla striata protocorm grows two panels cotyledon after planting to be sprayed water every 2~4 days to the bletilla striata
Afterwards, thunder power liquid seaweed extract and potassium dihydrogen phosphate were sprayed respectively every 4~7 days.
8. the method according to claim 7 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：The sea
The spraying concentration of algae solution fertilizer is 1500~1800 times.
9. the method according to claim 7 cultivated using bletilla striata direct sowing and seedling matrix, it is characterised in that：The phosphorus
The spraying concentration of acid dihydride potassium is 2000~2500 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710917429.0A CN107646591A (en) | 2017-09-30 | 2017-09-30 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710917429.0A CN107646591A (en) | 2017-09-30 | 2017-09-30 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107646591A true CN107646591A (en) | 2018-02-02 |
Family
ID=61116315
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710917429.0A Pending CN107646591A (en) | 2017-09-30 | 2017-09-30 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107646591A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220682A (en) * | 2018-08-03 | 2019-01-18 | 河南云帮农业科技有限公司 | A kind of management method after bletilla striata live streaming |
| CN113951118A (en) * | 2021-10-18 | 2022-01-21 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586164A (en) * | 2004-07-30 | 2005-03-02 | 中国科学院华南植物园 | Sterile seeding and tissue cultivating technology for Vanda |
| CN104255496A (en) * | 2014-09-23 | 2015-01-07 | 江苏农林职业技术学院 | Method for immediately propagating bletilla striata test-tube plantlet |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN105493861A (en) * | 2015-12-24 | 2016-04-20 | 陕西师范大学 | Method for directly seeding bletilla striata protocorms |
| CN105613234A (en) * | 2015-12-24 | 2016-06-01 | 陕西师范大学 | Direct seeding method of bletilla ochracea protocorm |
| CN106172005A (en) * | 2016-08-03 | 2016-12-07 | 济南麒麟花卉有限公司 | A kind of subculture medium and preparation method thereof |
| CN106358823A (en) * | 2016-08-29 | 2017-02-01 | 遵义联谷农业科技有限公司 | Bletilla striata culture medium and preparation method thereof |
| CN106417011A (en) * | 2016-08-30 | 2017-02-22 | 云南金碧制药有限公司 | Wild bletilla striata tissue culture rapid propagation method |
| CN106577002A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Bletilla striata culture medium, and preparation method and application thereof |
| CN107047316A (en) * | 2017-06-06 | 2017-08-18 | 郑州市农林科学研究所 | A kind of iris tissue culture method and culture medium |
| CN109329049A (en) * | 2018-09-28 | 2019-02-15 | 江苏农林职业技术学院 | A kind of method of bletilla tissue culture seedlings Mycorrhizal |
-
2017
- 2017-09-30 CN CN201710917429.0A patent/CN107646591A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1586164A (en) * | 2004-07-30 | 2005-03-02 | 中国科学院华南植物园 | Sterile seeding and tissue cultivating technology for Vanda |
| CN104255496A (en) * | 2014-09-23 | 2015-01-07 | 江苏农林职业技术学院 | Method for immediately propagating bletilla striata test-tube plantlet |
| CN105103928A (en) * | 2015-09-29 | 2015-12-02 | 江苏农林职业技术学院 | Method for acclimatizing and transplanting bletilla striata tissue culture seedlings |
| CN105493861A (en) * | 2015-12-24 | 2016-04-20 | 陕西师范大学 | Method for directly seeding bletilla striata protocorms |
| CN105613234A (en) * | 2015-12-24 | 2016-06-01 | 陕西师范大学 | Direct seeding method of bletilla ochracea protocorm |
| CN106172005A (en) * | 2016-08-03 | 2016-12-07 | 济南麒麟花卉有限公司 | A kind of subculture medium and preparation method thereof |
| CN106358823A (en) * | 2016-08-29 | 2017-02-01 | 遵义联谷农业科技有限公司 | Bletilla striata culture medium and preparation method thereof |
| CN106417011A (en) * | 2016-08-30 | 2017-02-22 | 云南金碧制药有限公司 | Wild bletilla striata tissue culture rapid propagation method |
| CN106577002A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Bletilla striata culture medium, and preparation method and application thereof |
| CN107047316A (en) * | 2017-06-06 | 2017-08-18 | 郑州市农林科学研究所 | A kind of iris tissue culture method and culture medium |
| CN109329049A (en) * | 2018-09-28 | 2019-02-15 | 江苏农林职业技术学院 | A kind of method of bletilla tissue culture seedlings Mycorrhizal |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109220682A (en) * | 2018-08-03 | 2019-01-18 | 河南云帮农业科技有限公司 | A kind of management method after bletilla striata live streaming |
| CN113951118A (en) * | 2021-10-18 | 2022-01-21 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
| CN113951118B (en) * | 2021-10-18 | 2023-02-17 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104719152B (en) | Rhizoma bletillae industrialized seedling method | |
| CN103314738B (en) | Dendrobium huoshanense greenhouse planting method | |
| CN104126397B (en) | Bletilla striata direct sowing and seedling method | |
| CN104429965B (en) | The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture | |
| CN104322257A (en) | Method for normatively planting dendrobium officinale in simple greenhouse | |
| CN108029431A (en) | Citrus grafting seedlings-growing method | |
| CN105594567A (en) | Planting method for Dendrobium officinale Kimura et Migo | |
| CN102668959A (en) | Rapid ex vitro rooting method for blueberry tissue culture seedlings and rooting culture matrix | |
| CN105123529A (en) | Rapid propagation and efficient cultivation method of Bletilla striata | |
| CN102630449A (en) | High-yield cultivation method of field pumpkin | |
| CN104221664B (en) | A kind of maca normalized planting method | |
| CN105191625A (en) | Pollution-free high-yield cultivation method for sweet corn | |
| CN105638371B (en) | A kind of green propagation method of Gu tea tree | |
| CN103098712A (en) | Davallia mariesii breeding method | |
| CN103430755A (en) | Method for developing healthy sugarcane seeds | |
| CN107646591A (en) | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix | |
| CN105027955A (en) | Planting method for yellow bell peppers | |
| CN102640634A (en) | Method for cultivating summer squash by using biogas manure | |
| CN105028213A (en) | Tissue-culturing rapid propagation method for dendrobium officinale | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN107155841A (en) | A kind of water planting propagation method of leek | |
| CN106818175A (en) | A kind of bletilla floating plate method for culturing seedlings | |
| CN104620801A (en) | Technical method for cowpea planting | |
| CN103583196A (en) | Method for cultivating greenhouse thin-skin melons | |
| CN101617596A (en) | Method for cutting culture of Chinese artichoke |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180202 |