CN106172005A - A kind of subculture medium and preparation method thereof - Google Patents
A kind of subculture medium and preparation method thereof Download PDFInfo
- Publication number
- CN106172005A CN106172005A CN201610627498.3A CN201610627498A CN106172005A CN 106172005 A CN106172005 A CN 106172005A CN 201610627498 A CN201610627498 A CN 201610627498A CN 106172005 A CN106172005 A CN 106172005A
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- China
- Prior art keywords
- subculture medium
- activated carbon
- peptone
- glycine
- medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of subculture medium, every liter of culture medium is made up of following components: spend precious 5 15g；Os Bovis seu Bubali peptone 0.8 1.2g；Activated carbon 1 3g；Rhizoma Solani tuber osi 40 60g；Fructus Musae 20 30g；Sucrose 3 6g；Glycine 80 120g；Nicotinic acid 23 μ gVB1 0.3 0.6vg.The subculture medium component comprehensive nutrition that the present invention provides, the Herba Dendrobii Sprouting rate of cultivation is fast, and gemmule is full, is effectively improved the quality sprouted.Meanwhile, each component is simple, and source is wide to be sent out, it is easy to obtain, cheap, is especially suitable for extensive tissue culture.
Description
Technical field
The present invention relates to a kind of subculture medium and preparation method thereof.
Background technology
Herba Dendrobii is a kind of medicinal plants extremely treasured, and has the medical value of uniqueness.Due to Seeds of Dendrobium Candidum
Germination percentage is low, and division propagation speed is slow, have impact on the process of large-scale production, and the transplanting survival rate of test tube Seedling is low in addition, causes
Tense situation between Herba Dendrobii medicine source supply and demand.In recent years, the tissue culture technique of Herba Dendrobii becomes the focus of research.At present
On market, the culture medium about candidum tissue culturing has a variety of, and wherein component content is also not quite similar, but Herba Dendrobii goes out
The speed of bud is undesirable, and gemmule is the fullest, and root system is insufficient, governs development and the iron sheet of the tissue culture technology of Herba Dendrobii
The market sale of Herba Dendrobii.
Summary of the invention
For drawbacks described above and deficiency present in prior art, the invention provides a kind of subculture medium and preparation thereof
Method.
The present invention is achieved by the following technical solution: a kind of subculture medium, every liter of culture medium is by following components
Make:
Spend a precious 5-15g；
Os Bovis seu Bubali peptone 0.8-1.2g；
Activated carbon 1-3g；
Rhizoma Solani tuber osi 40-60g；
Fructus Musae 20-30g；
Sucrose 3-6g；
Glycine 80-120g；
Nicotinic acid 2-3 μ g；
VB1 0.3-0.6vg。
As preferably, every liter of culture medium is made up of following components:
Spend a precious 10.0g；
Os Bovis seu Bubali peptone 1.0g；
Activated carbon 2.0g；
Rhizoma Solani tuber osi 50.0g；
Fructus Musae 25.0g；
Sucrose 5.0g；
Glycine 100.0g
Nicotinic acid 2.5 μ g；
VB1 0.5vg。
It also includes following preparation method:
1) each component in subculture medium city as described in configuration 10L；
2) Rhizoma Solani tuber osi, Fructus Musae stirring into mud respectively, activated carbon 100ml distilled water dissolves standby；
3) add distilled water 5L in pot, boiled after put into agar 5.5g/L, be heated up to agar dissolve, be then placed in mashed potato and perfume (or spice)
Any of several broadleaf plants mud, continues to heat, and puts into Os Bovis seu Bubali peptone；
4) put into yellow seed 5g/L, glycine, nicotinic acid, VB1, activated carbon after off the pot, be then dissolved to 10L；
5) regulation pH value is to 5.6；
6) it is dispensed in tissue culture bottle, every bottle of 100ml, tissue culture bottle is put in high-pressure sterilizing pot；
7) tissue culture bottle after autoclaving carries out ultraviolet disinfection after 20 minutes and to get final product.
As preferably, in described preparation method, the temperature of step 6) mesohigh autoclave is 210-220 DEG C.
The subculture medium component comprehensive nutrition that the present invention provides, the Herba Dendrobii Sprouting rate of cultivation is fast, and gemmule is full,
It is effectively improved the quality sprouted.Meanwhile, each component is simple, and source is wide to be sent out, it is easy to obtain, cheap, is especially suitable for extensive
Tissue culture.
Detailed description of the invention
Below by the embodiment of indefiniteness to invention further instruction:
Embodiment 1
A kind of subculture medium, every liter of culture medium is made up of following components:
Spend a precious 10.0g；
Os Bovis seu Bubali peptone 1.0g；
Activated carbon 2.0g；
Rhizoma Solani tuber osi 50.0g；
Fructus Musae 25.0g；
Sucrose 5.0g；
Glycine 100.0g
Nicotinic acid 2.5 μ g；
VB1 0.5vg。
Embodiment 2
A kind of subculture medium, every liter of culture medium is made up of following components:
Spend a precious 5g；
Os Bovis seu Bubali peptone 0.8g；
Activated carbon 1g；
Rhizoma Solani tuber osi 40g；
Fructus Musae 20g；
Sucrose 3g；
Glycine 80g；
Nicotinic acid 2 μ g；
VB1 0.3vg。
Embodiment 3
A kind of subculture medium, every liter of culture medium is made up of following components:
Spend a precious 15g；
Os Bovis seu Bubali peptone 1.2g；
Activated carbon 3g；
Rhizoma Solani tuber osi 60g；
Fructus Musae 30g；
Sucrose 6g；
Glycine 120g；
Nicotinic acid 3 μ g；
VB1 0.6vg。
It also includes following preparation method:
1) each component in subculture medium city as described in configuration 10L；
2) Rhizoma Solani tuber osi, Fructus Musae stirring into mud respectively, activated carbon 100ml distilled water dissolves standby；
3) add distilled water 5L in pot, boiled after put into agar 5.5g/L, be heated up to agar dissolve, be then placed in mashed potato and perfume (or spice)
Any of several broadleaf plants mud, continues to heat, and puts into Os Bovis seu Bubali peptone；
4) put into yellow seed 5g/L, glycine, nicotinic acid, VB1, activated carbon after off the pot, be then dissolved to 10L；
5) regulation pH value is to 5.6；
6) it is dispensed in tissue culture bottle, every bottle of 100ml, tissue culture bottle is put in high-pressure sterilizing pot；
7) tissue culture bottle after autoclaving carries out ultraviolet disinfection after 20 minutes and to get final product.
As preferably, in described preparation method, the temperature of step 6) mesohigh autoclave is 210-220 DEG C.
The above, only several embodiments of the present invention, it is impossible to limit protection scope of the present invention according to this, any
Rely on the equivalent transformation done by the present invention and modification, broadly fall in protection scope of the present invention.
Claims (4)
1. a subculture medium, it is characterised in that: every liter of culture medium is made up of following components:
Spend a precious 5-15g；
Os Bovis seu Bubali peptone 0.8-1.2g；
Activated carbon 1-3g；
Rhizoma Solani tuber osi 40-60g；
Fructus Musae 20-30g；
Sucrose 3-6g；
Glycine 80-120g；
Nicotinic acid 2-3 μ g；
VB1 0.3-0.6vg。
A kind of subculture medium the most according to claim 1, it is characterised in that: every liter of culture medium is made up of following components:
Spend a precious 10.0g；
Os Bovis seu Bubali peptone 1.0g；
Activated carbon 2.0g；
Rhizoma Solani tuber osi 50.0g；
Fructus Musae 25.0g；
Sucrose 5.0g；
Glycine 100.0g
Nicotinic acid 2.5 μ g；
VB1 0.5vg。
3. according to a kind of subculture medium described in claims 1, it is characterised in that: it also includes following preparation method:
1) each component in subculture medium city as described in configuration 10L；
2) Rhizoma Solani tuber osi, Fructus Musae stirring into mud respectively, activated carbon 100ml distilled water dissolves standby；
3) add distilled water 5L in pot, boiled after put into agar 5.5g/L, be heated up to agar dissolve, be then placed in mashed potato and perfume (or spice)
Any of several broadleaf plants mud, continues to heat, and puts into Os Bovis seu Bubali peptone；
4) put into yellow seed 5g/L, glycine, nicotinic acid, VB1, activated carbon after off the pot, be then dissolved to 10L；
5) regulation pH value is to 5.6；
6) it is dispensed in tissue culture bottle, every bottle of 100ml, tissue culture bottle is put in high-pressure sterilizing pot；
7) tissue culture bottle after autoclaving carries out ultraviolet disinfection after 20 minutes and to get final product.
A kind of subculture medium the most according to claim 3, it is characterised in that: high in step 6) in described preparation method
The temperature of pressure autoclave is 210-220 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610627498.3A CN106172005A (en) | 2016-08-03 | 2016-08-03 | A kind of subculture medium and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610627498.3A CN106172005A (en) | 2016-08-03 | 2016-08-03 | A kind of subculture medium and preparation method thereof |
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| CN106172005A true CN106172005A (en) | 2016-12-07 |
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| CN201610627498.3A Pending CN106172005A (en) | 2016-08-03 | 2016-08-03 | A kind of subculture medium and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
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| CN101213941A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication and in-vitro conservation method for dendrobium |
| CN101461328A (en) * | 2009-01-08 | 2009-06-24 | 中国科学院华南植物园 | High-efficient propagation method of seedlings of Dendrobium officinale |
| CN101569287A (en) * | 2009-06-02 | 2009-11-04 | 中国科学院华南植物园 | Method for test tube propagation of seedlings of zygopetalum spp. |
| CN102144536A (en) * | 2011-03-09 | 2011-08-10 | 中国科学院华南植物园 | Method for crossbreeding and aseptic sowing of renanthera coccinea |
| CN104145815A (en) * | 2014-07-25 | 2014-11-19 | 庄国钟 | Dendrobium candidum culture medium and preparation method thereof |
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2016
- 2016-08-03 CN CN201610627498.3A patent/CN106172005A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101213941A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication and in-vitro conservation method for dendrobium |
| CN101461328A (en) * | 2009-01-08 | 2009-06-24 | 中国科学院华南植物园 | High-efficient propagation method of seedlings of Dendrobium officinale |
| CN101569287A (en) * | 2009-06-02 | 2009-11-04 | 中国科学院华南植物园 | Method for test tube propagation of seedlings of zygopetalum spp. |
| CN102144536A (en) * | 2011-03-09 | 2011-08-10 | 中国科学院华南植物园 | Method for crossbreeding and aseptic sowing of renanthera coccinea |
| CN104145815A (en) * | 2014-07-25 | 2014-11-19 | 庄国钟 | Dendrobium candidum culture medium and preparation method thereof |
Non-Patent Citations (4)
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| 方香香等: "霍山石斛快速繁殖技术研究", 《中国现代中药》 * |
| 林俊达等: "铁皮石斛增殖培养培养基配方的优化", 《东南园艺》 * |
| 蔡朝晖等: "培养基中蔗糖浓度及添加氨基酸对组培暗紫贝母生长的影响", 《中国药科大学学报》 * |
| 陈美霞: "《植物组织培养》", 31 August 2012, 华中科技大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646591A (en) * | 2017-09-30 | 2018-02-02 | 江苏农林职业技术学院 | A kind of bletilla striata direct sowing and seedling matrix and the method cultivated using the matrix |
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Application publication date: 20161207 |