CN107056414A - A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two - Google Patents

A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two Download PDF

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Publication number
CN107056414A
CN107056414A CN201710058484.9A CN201710058484A CN107056414A CN 107056414 A CN107056414 A CN 107056414A CN 201710058484 A CN201710058484 A CN 201710058484A CN 107056414 A CN107056414 A CN 107056414A
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CN
China
Prior art keywords
culture medium
broad spectrum
fruit pod
spectrum activity
aseptic seeding
Prior art date
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Pending
Application number
CN201710058484.9A
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Chinese (zh)
Inventor
郑宝强
王雁
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Research Institute of Forestry of Chinese Academy of Forestry
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Research Institute of Forestry of Chinese Academy of Forestry
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Publication date
Application filed by Research Institute of Forestry of Chinese Academy of Forestry filed Critical Research Institute of Forestry of Chinese Academy of Forestry
Priority to CN201710058484.9A priority Critical patent/CN107056414A/en
Publication of CN107056414A publication Critical patent/CN107056414A/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two, include per L culture mediums：NH4NO3 120g、KNO3 34g、CaCl2·2H2O 440g、MgSO4·7H2O 370g、KH2PO490g, sucrose 20g, activated carbon 2g, agar 5.8g, remaining is 1/4MS trace element, organic principle and molysite, pH=5.7.Ripe Fruit pod period is taken to be cultivated：The Fruit pod that do not ftracture is taken, Fruit pod carpopodium position starts to take when turning yellow.24 to 26 DEG C of cultivation temperature, intensity of illumination is 60 μm of olm‑2s‑1, hour/day of light application time 12.Using the culture medium, Paphiopedilum seed germination rate is more than 70%, and Bowring cattleya category, Phalaenopsis, dendrobium seed germination rate are more than 90%, and other hot bandwidths sowing seed germination rate is also all more than 80%.

Description

A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two
Technical field
The present invention relates to a kind of flowers culture medium, more particularly to a kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two.
Background technology
Orchid is one of big famous flower of China ten, is also one of world's rare flower, occupies particularly significant in the production of flowers and plants Status.With it is economical develop rapidly, the quickening and the improvement of people's living standards of urbanization process, traditional orchid is Can not meet the growing aesthetic requirement of people, various hot bandwidths, due to cultivation easily, it is easily strange into flower, pattern Gorgeous, flower-shape is changeable, the florescence is permanent, various colors are colorful, and the favorite of market flowers is had become at present.
A large amount of seedling can be obtained by aseptic seeding, be the Main Means that new varieties are cultivated in crossbreeding.But at present There is the defect of broad spectrum activity difference through disclosed culture medium, a kind of general sowing culture medium of each hot bandwidth correspondence uses other The orchid sowing culture medium seed of species can not sprout or germination rate is low.
The content of the invention
It is an object of the invention to provide a kind of high broad spectrum activity hot bandwidth aseptic seeding culture medium two of seed germination rate.
The purpose of the present invention is achieved through the following technical solutions：
The broad spectrum activity hot bandwidth aseptic seeding culture medium two of the present invention, includes per L culture mediums：
NH4NO3120g、KNO334g、CaCl2·2H2O 440g、MgSO4·7H2O 370g、KH2PO490g, sucrose 20g, Activated carbon 2g, agar 5.8g, remaining is 1/4MS trace element, organic principle and molysite, pH=5.7.
As seen from the above technical solution provided by the invention, broad spectrum activity hot bandwidth provided in an embodiment of the present invention is sterile Culture medium two is sowed, makes Paphiopedilum seed germination rate more than 70%, Bowring cattleya category, Phalaenopsis, dendrobium seed are sprouted Rate is more than 90%, and other hot bandwidths sowing seed germination rate is also all more than 80%.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment Only a part of embodiment of the invention, rather than whole embodiments.Based on embodiments of the invention, ordinary skill The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to protection scope of the present invention.
The broad spectrum activity hot bandwidth aseptic seeding culture medium two of the present invention, its preferably embodiment is：
Include per L culture mediums：
NH4NO3120g、KNO334g、CaCl2·2H2O 440g、MgSO4·7H2O 370g、KH2PO490g, sucrose 20g, Activated carbon 2g, agar 5.8g, remaining is 1/4MS trace element, organic principle and molysite, pH=5.7.
Ripe Fruit pod period is taken to be cultivated：The Fruit pod that do not ftracture is taken, Fruit pod carpopodium position starts to take when turning yellow.
24 to 26 DEG C of cultivation temperature, intensity of illumination is 60 μm of olm-2s-1, hour/day of light application time 12.
The embodiment of the present invention will be described in further detail below.
Ripe Fruit pod picking time：Fruit pod does not ftracture, when Fruit pod carpopodium position starts to turn yellow.
Culture medium is：NH4NO3 120g/L+KNO334g/L+CaCl2·2H2O 440g/L+MgSO4·7H2O 370g/L+ KH2PO4120g/L+1/4MS trace elements, organic principle, molysite+sucrose 20g/L+ activated carbon 2g/L+ agar 5.8g/L, pH= 5.7。
25 DEG C or so of cultivation temperature, intensity of illumination is 60 μm of olm-2s-1, hour/day of light application time 12.
Specific embodiment：
(1) for aseptic seeding Fruit pod with it is just ripe but when not yet ftractureing effect preferably, general Fruit pod carpopodium position is opened When beginning to turn yellow preferably.At this moment seed can be separated from each other in capsule, easily sowing.One is all sticked in during immature seed sowing Rise, be unfavorable for late growing stage.Uncracked Fruit pod sterilizing is easy in addition, and only Fruit pod epidermis can be sterilized；After Fruit pod cracking, powder Shape Seed sterilization is more difficult, and Seed germinating ability is reduced.Preferably it is to adopt to broadcast after Fruit pod harvesting, has not enough time to sowing time, Ke Yiyong Sealed polybag is installed to be put into 4 DEG C of refrigerator and preserved, but the time should not too long, otherwise easy mildew or reduction germination percentage.
(2) aseptic seeding must be carried out on superclean bench, and all medicaments and instrument are got out before operation, be kept The germ-free condition of superclean bench.
(3) uncracked pod is removed into persitent perianth, stamen post, carpopodium, after being cleaned through running water, is placed in 10% hypochlorous acid Sterilized 20 minutes in sodium solution, with 75% alcohol surface sterilization 3 minutes, calcination removes pod surface wine directly on alcolhol burner Essence, can thus complete sterilization.
(4) Fruit pod is splitted, seed is uniformly sprinkling upon media surface.
(5) hot bandwidth seed sprouts very fast in 25 DEG C or so germination and growths, the too high easy Necrosis of temperature, the too low hair of temperature Bud is slow.When temperature is less than 10 DEG C, even if meeting nutritional condition can not sprout.Intensity of illumination is 60 μm of olm-2s-1, illumination Time 12 hour/day.
Culture medium more than use is sowed, Paphiopedilum seed germination rate is more than 70%, Bowring cattleya category, Phalaenopsis, the stem of noble dendrobium Cymbidium seed germination rate is more than 90%, and other hot bandwidths sowing seed germination rate is also all more than 80%.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto, Any one skilled in the art is in the technical scope of present disclosure, the change or replacement that can be readily occurred in, It should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims Enclose and be defined.

Claims (3)

1. a kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two, it is characterised in that include per L culture mediums：
NH4NO3 120g、KNO3 34g、CaCl2·2H2O 440g、MgSO4·7H2O 370g、KH2PO490g, sucrose 20g, work Property charcoal 2g, agar 5.8g, remaining is 1/4MS trace element, organic principle and molysite, pH=5.7.
2. broad spectrum activity hot bandwidth aseptic seeding culture medium two according to claim 1, it is characterised in that take ripe Fruit pod Period is cultivated：The Fruit pod that do not ftracture is taken, Fruit pod carpopodium position starts to take when turning yellow.
3. broad spectrum activity hot bandwidth aseptic seeding culture medium two according to claim 2, it is characterised in that cultivation temperature 24 to 26 DEG C, intensity of illumination is 60 μm of olm-2s-1, hour/day of light application time 12.
CN201710058484.9A 2017-01-23 2017-01-23 A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two Pending CN107056414A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710058484.9A CN107056414A (en) 2017-01-23 2017-01-23 A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710058484.9A CN107056414A (en) 2017-01-23 2017-01-23 A kind of broad spectrum activity hot bandwidth aseptic seeding culture medium two

Publications (1)

Publication Number Publication Date
CN107056414A true CN107056414A (en) 2017-08-18

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109328985A (en) * 2018-11-22 2019-02-15 安徽祥峰生物科技有限公司 It is a kind of promote Dendrobidium huoshanness seed sprout culture medium and type of seeding
CN110663549A (en) * 2019-09-24 2020-01-10 福建省农业科学院作物研究所 Sterile sowing and seedling raising method for dendrobium hybrid seeds
WO2020171786A1 (en) * 2019-02-19 2020-08-27 T.C. Tarim Ve Orman Bakanliği Doğu Akdeni̇z Geçi̇t Kuşaği Tarimsal Araştirma Ensti̇tüsü Müdürlüğü A new nutrient media and method ensuring the multiplication of anatolian orchids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101352117A (en) * 2008-08-15 2009-01-28 中国科学院植物研究所 Culture medium of germination of orchid seed
CN104186295A (en) * 2014-09-29 2014-12-10 佛山市顺德区今日景艺生物科技有限公司 Culture medium for paphiopedilum seed germination and culture method
CN105052750A (en) * 2015-09-08 2015-11-18 广西壮族自治区农业科学院花卉研究所 Crossbreeding and seedling rapid propagation method of paphiopedilum pacific shamrock

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101352117A (en) * 2008-08-15 2009-01-28 中国科学院植物研究所 Culture medium of germination of orchid seed
CN104186295A (en) * 2014-09-29 2014-12-10 佛山市顺德区今日景艺生物科技有限公司 Culture medium for paphiopedilum seed germination and culture method
CN105052750A (en) * 2015-09-08 2015-11-18 广西壮族自治区农业科学院花卉研究所 Crossbreeding and seedling rapid propagation method of paphiopedilum pacific shamrock

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
陈菁瑛等: "《兰花组织培养与快速繁殖技术》", 31 October 2004 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109328985A (en) * 2018-11-22 2019-02-15 安徽祥峰生物科技有限公司 It is a kind of promote Dendrobidium huoshanness seed sprout culture medium and type of seeding
WO2020171786A1 (en) * 2019-02-19 2020-08-27 T.C. Tarim Ve Orman Bakanliği Doğu Akdeni̇z Geçi̇t Kuşaği Tarimsal Araştirma Ensti̇tüsü Müdürlüğü A new nutrient media and method ensuring the multiplication of anatolian orchids
CN110663549A (en) * 2019-09-24 2020-01-10 福建省农业科学院作物研究所 Sterile sowing and seedling raising method for dendrobium hybrid seeds

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Application publication date: 20170818