CN105191797A - Snakegourd fruit tissues culture method - Google Patents
Snakegourd fruit tissues culture method Download PDFInfo
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- CN105191797A CN105191797A CN201510632543.XA CN201510632543A CN105191797A CN 105191797 A CN105191797 A CN 105191797A CN 201510632543 A CN201510632543 A CN 201510632543A CN 105191797 A CN105191797 A CN 105191797A
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Abstract
The invention discloses a snakegourd fruit tissues culture method, which comprises steps: environmental disinfection, selection and processing of explants, preparation of culture mediums, inoculation of the explants, cultivation of culture rooms, greenhouse and greenhouse seedling cultivation and transplant. The snakegourd fruit tissues culture method greatly reduces growing-seedling period and growing-seedling survival rate by culturing nakegourd fruit tissues. The snakegourd fruit tissues culture method has the beneficial effects that the snakegourd fruit tissues culture method is simple and effective, is excellent in disinfection effect of the explants, and can achieve above 95% sterility rate after the explants are disinfected. The explants sprout and grow in aseptic conditions, overcome the influence of environment, improve survival rate of seedling emergence, and are transplanted to plant in seeding trays and grow in a greenhouse after small seeding grows to a certain length, thereby enabling quality of snakegourd fruits to be excellent and healthy, and greatly improving survival rate of field production of the snakegourd fruits,.
Description
Technical field
The present invention relates to Plant Biotechnology and technical field of cultivation, specifically a kind of Snakegourd Fruit tissue culture method.
Background technology
Snakegourd Fruit, the perennial climbing herb of Curcurbitaceae snake gourd, length can reach 10 meters.Root-like stock is plump, cylindric, and crust is yellow.Stem multi-branched, without hair; Leaf alternate, subcircular or heart, dioecism; A male flower number raceme, rare Dan Sheng, corolla lobe obovate, female flower Dan Sheng, ovary is avette, and fruit is subsphaeroidal, orange red time ripe, 7 ~ November of flowering fruit bearing stage.
Snakegourd Fruit have antipyreticly to quench the thirst, diuresis, the effect such as kobadrin.Seed is containing fat flower fat oil; Fruit is containing triterpenoid saponin, organic acid, resin, carbohydrate, pigment; Root is containing protein, saponin(e, acids.Snakegourd Fruit rich in nutritive value, is rich in nutrient component, and wherein protein, fat, cellulose, trace element and alkaloid, flavonoids, infantile malnutrition due to digestive disturbances or intestinalparasites class and organic equal size are rich.There is efficiency in current trichosanthes fruit seedling planting process lower, mainly comprise following aspect: one, Snakegourd Fruit tissue culture technology is imperfect, not complete, and in production process, dead seedling, rotten seedling phenomenon are seriously, under production efficiency; Its two, hormone concentration improper use in tissue culture process, uses the too low Snakegourd Fruit reproductive efficiency that causes low, uses the too high Snakegourd Fruit that causes to produce malformed flower, not even Flowering Phenomenon; Its three, existing tissue culture method is taken root comparatively slow, and easily bad root phenomenon occurs.
Summary of the invention
The present invention aims to provide a kind of Snakegourd Fruit tissue culture method, and overcome the deficiencies in the prior art, Disinfection Effect of the present invention is good, and rootage duration is short, and growth time is fast, and seedling quality is high.
The invention provides following technical scheme:
A kind of Snakegourd Fruit tissue culture method, comprises the following steps:
(1) environment disinfected: by group training operating room, culturing room's sterilization, is then placed on HD-850 type table above formula horizontal blast clean bench in group training operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace;
(2) the choosing and processing of explant: choose bud or rattan shape stem that Snakegourd Fruit block root is sprouted as explant, by explant running water running water 20min, after adding washing agent soaking and washing 20min, use running water running water 30min again, explant after cleaning is moved in the beaker of super-clean bench, add 75% ethanol disinfection 30s, again with aqua sterilisa cleaning 2-3 time, explant is joined the mercuric chloride solution sterilization 8-15min of 0.1%, again with aqua sterilisa cleaning 6-10 time, blot surface moisture with sterile gauze, cut off stem section two ends a little;
(3) medium preparing: add 30g/L white sugar at MS culture medium prescription, 0.5mg/LNAA hormone, 6g/L agar, loads blake bottle, and regulate pH value 5.8-6.0 with NaOH or HCl, 121 DEG C of high-temp steam sterilizings cool for subsequent use after 20 minutes;
(4) explant inoculation: by the above-mentioned bud through sterilization or stem section, be seeded according to biology polar orientation and be equipped with in the blake bottle of medium, every bottle of bud or a stem section;
(6) culturing room cultivates: after sterilization in greenhouse, culturing room adopts fluorescent lamp to carry out illumination, control temperature is at 20-25 DEG C, humidity more than 60%, illumination 1500-2500lx, illumination 10-12h, cultivate one month, can grow up to 4-5 and save stem with leaves, need replaced medium to carry out sub-bottle after 2 months, continue to cultivate 3-4 after replaced medium and get final product bottle outlet hardening in individual month;
(6) booth hardening is cultivated: taken out from blake bottle by seedling, by 800-1000 times of carbendazol soaking and washing 2-3 time, is washed off by the medium of root, and then plantation is arrived in compost seedling dish; The seedling dish of having planted Snakegourd Fruit seedling is put into experienced seedling booth and cultivate 2-3 month, dish seedling keeps humidity more than 80%, temperature 20-30 DEG C in early days, later stage is stronger ventilation amount gradually, sprays water-soluble good compound fertilizer, notes preventing disease and pest, adopt tidal irrigation, and regularly spray foliage fertilizer;
(7) transplant: cultivate through booth hardening and be implanted into soil self-sow after two months.
Compared with prior art, the invention has the beneficial effects as follows: sterilization method of the present invention is simple, effectively, explant Disinfection Effect is good, after explant sterilization, aseptic rate can reach more than 95%; Carry out sprout growth in an aseptic environment, overcome the impact of environment, improve seedling emergence survival rate, shift out plantation after band seedling grows to certain length and grow in booth to seedling dish, the Snakegourd Fruit seedling stalwartness best in quality obtained, substantially increases the Production of Large Fields survival rate of Snakegourd Fruit.
Embodiment
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
A kind of Snakegourd Fruit tissue culture method, comprises the following steps:
(1) environment disinfected: by group training operating room, culturing room's sterilization, is then placed on HD-850 type table above formula horizontal blast clean bench in group training operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace;
(2) the choosing and processing of explant: choose bud or rattan shape stem that Snakegourd Fruit block root is sprouted as explant, by explant running water running water 20min, after adding washing agent soaking and washing 20min, use running water running water 30min again, explant after cleaning is moved in the beaker of super-clean bench, add 75% ethanol disinfection 30s, again with aqua sterilisa cleaning 2-3 time, explant is joined the mercuric chloride solution sterilization 8-15min of 0.1%, again with aqua sterilisa cleaning 6-10 time, blot surface moisture with sterile gauze, cut off stem section two ends a little;
(3) medium preparing: add 30g/L white sugar at MS culture medium prescription, 0.5mg/LNAA hormone, 6g/L agar, loads blake bottle, and regulate pH value 5.8-6.0 with NaOH or HCl, 121 DEG C of high-temp steam sterilizings cool for subsequent use after 20 minutes;
(4) explant inoculation: by the above-mentioned bud through sterilization or stem section, be seeded according to biology polar orientation and be equipped with in the blake bottle of medium, every bottle of bud or a stem section;
(7) culturing room cultivates: after sterilization in greenhouse, culturing room adopts fluorescent lamp to carry out illumination, control temperature is at 20-25 DEG C, humidity more than 60%, illumination 1500-2500lx, illumination 10-12h, cultivate one month, can grow up to 4-5 and save stem with leaves, need replaced medium to carry out sub-bottle after 2 months, continue to cultivate 3-4 after replaced medium and get final product bottle outlet hardening in individual month;
(6) booth hardening is cultivated: taken out from blake bottle by seedling, by 800-1000 times of carbendazol soaking and washing 2-3 time, is washed off by the medium of root, and then plantation is arrived in compost seedling dish; The seedling dish of having planted Snakegourd Fruit seedling is put into experienced seedling booth and cultivate 2-3 month, dish seedling keeps humidity more than 80%, temperature 20-30 DEG C in early days, later stage is stronger ventilation amount gradually, sprays water-soluble good compound fertilizer, notes preventing disease and pest, adopt tidal irrigation, and regularly spray foliage fertilizer;
(7) transplant: cultivate through booth hardening and be implanted into soil self-sow after two months.
Claims (1)
1. a Snakegourd Fruit tissue culture method, is characterized in that, comprises the following steps:
(1) environment disinfected: by group training operating room, culturing room's sterilization, is then placed on HD-850 type table above formula horizontal blast clean bench in group training operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace;
(2) the choosing and processing of explant: choose bud or rattan shape stem that Snakegourd Fruit block root is sprouted as explant, by explant running water running water 20min, after adding washing agent soaking and washing 20min, use running water running water 30min again, explant after cleaning is moved in the beaker of super-clean bench, add 75% ethanol disinfection 30s, again with aqua sterilisa cleaning 2-3 time, explant is joined the mercuric chloride solution sterilization 8-15min of 0.1%, again with aqua sterilisa cleaning 6-10 time, blot surface moisture with sterile gauze, cut off stem section two ends a little;
(3) medium preparing: add 30g/L white sugar at MS culture medium prescription, 0.5mg/LNAA hormone, 6g/L agar, loads blake bottle, and regulate pH value 5.8-6.0 with NaOH or HCl, 121 DEG C of high-temp steam sterilizings cool for subsequent use after 20 minutes;
(4) explant inoculation: by the above-mentioned bud through sterilization or stem section, be seeded according to biology polar orientation and be equipped with in the blake bottle of medium, every bottle of bud or a stem section;
(5) culturing room cultivates: after sterilization in greenhouse, culturing room adopts fluorescent lamp to carry out illumination, control temperature is at 20-25 DEG C, humidity more than 60%, illumination 1500-2500lx, illumination 10-12h, cultivate one month, can grow up to 4-5 and save stem with leaves, need replaced medium to carry out sub-bottle after 2 months, continue to cultivate 3-4 after replaced medium and get final product bottle outlet hardening in individual month;
(6) booth hardening is cultivated: taken out from blake bottle by seedling, by 800-1000 times of carbendazol soaking and washing 2-3 time, is washed off by the medium of root, and then plantation is arrived in compost seedling dish; The seedling dish of having planted Snakegourd Fruit seedling is put into experienced seedling booth and cultivate 2-3 month, dish seedling keeps humidity more than 80%, temperature 20-30 DEG C in early days, later stage is stronger ventilation amount gradually, sprays water-soluble good compound fertilizer, notes preventing disease and pest, adopt tidal irrigation, and regularly spray foliage fertilizer;
(7) transplant: cultivate through booth hardening and be implanted into soil self-sow after two months.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069021A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling |
| CN106962189A (en) * | 2017-03-13 | 2017-07-21 | 蚌埠清菲农业科技有限公司 | A kind of method for promoting Snakegourd Fruit tissue culture to break up |
| CN107047300A (en) * | 2017-03-20 | 2017-08-18 | 蚌埠清菲农业科技有限公司 | A kind of tissue culture method of Snakegourd Fruit |
| CN107079677A (en) * | 2017-03-13 | 2017-08-22 | 蚌埠清菲农业科技有限公司 | A kind of method of promotion Snakegourd Fruit root division |
| CN107593461A (en) * | 2017-11-14 | 2018-01-19 | 李正美 | A kind of tissue culture rapid propagating technology of root of Chinese trichosanthes |
| CN108812311A (en) * | 2018-06-06 | 2018-11-16 | 周庆友 | A kind of method of snakegourd quick reproduction technique |
| CN108967194A (en) * | 2018-08-07 | 2018-12-11 | 遵义华富生物科技有限公司 | A kind of Chinese medicine melon withers the method for tissue-culturing rapid propagation seedling |
| CN109089884A (en) * | 2018-08-31 | 2018-12-28 | 湖州德清玖沐农业科技有限公司 | A kind of quick-breeding method of snakegourd seedling |
| CN112586353A (en) * | 2020-12-14 | 2021-04-02 | 颐正源（天津）科技有限公司 | Snakegourd fruit tissue culture seedling method and planting equipment thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985582A (en) * | 2006-12-18 | 2007-06-27 | 江苏阳光生态农林开发股份有限公司 | Tissue culture seedling growing process for trichosanthes |
| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
| CN103598097A (en) * | 2013-11-18 | 2014-02-26 | 绍兴市农业科学研究院 | Culture method of virus-free snake gourd tissue culture seedling |
| CN104145816A (en) * | 2014-07-29 | 2014-11-19 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue-culture seedling raising method |
| CN104521756A (en) * | 2014-12-24 | 2015-04-22 | 广西大学 | Method for producing trichosanthes tissue culture seedlings |
-
2015
- 2015-09-29 CN CN201510632543.XA patent/CN105191797A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985582A (en) * | 2006-12-18 | 2007-06-27 | 江苏阳光生态农林开发股份有限公司 | Tissue culture seedling growing process for trichosanthes |
| CN101699989A (en) * | 2009-11-20 | 2010-05-05 | 杨保成 | Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim |
| CN103598097A (en) * | 2013-11-18 | 2014-02-26 | 绍兴市农业科学研究院 | Culture method of virus-free snake gourd tissue culture seedling |
| CN104145816A (en) * | 2014-07-29 | 2014-11-19 | 郎溪县紫兰药材种植专业合作社 | Bletilla striata tissue-culture seedling raising method |
| CN104521756A (en) * | 2014-12-24 | 2015-04-22 | 广西大学 | Method for producing trichosanthes tissue culture seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 张龙霏等: "栝楼组织培养研究进展", 《山东中医药大学学报》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069021A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling |
| CN106962189A (en) * | 2017-03-13 | 2017-07-21 | 蚌埠清菲农业科技有限公司 | A kind of method for promoting Snakegourd Fruit tissue culture to break up |
| CN107079677A (en) * | 2017-03-13 | 2017-08-22 | 蚌埠清菲农业科技有限公司 | A kind of method of promotion Snakegourd Fruit root division |
| CN107047300A (en) * | 2017-03-20 | 2017-08-18 | 蚌埠清菲农业科技有限公司 | A kind of tissue culture method of Snakegourd Fruit |
| CN107593461A (en) * | 2017-11-14 | 2018-01-19 | 李正美 | A kind of tissue culture rapid propagating technology of root of Chinese trichosanthes |
| CN108812311A (en) * | 2018-06-06 | 2018-11-16 | 周庆友 | A kind of method of snakegourd quick reproduction technique |
| CN108967194A (en) * | 2018-08-07 | 2018-12-11 | 遵义华富生物科技有限公司 | A kind of Chinese medicine melon withers the method for tissue-culturing rapid propagation seedling |
| CN109089884A (en) * | 2018-08-31 | 2018-12-28 | 湖州德清玖沐农业科技有限公司 | A kind of quick-breeding method of snakegourd seedling |
| CN112586353A (en) * | 2020-12-14 | 2021-04-02 | 颐正源（天津）科技有限公司 | Snakegourd fruit tissue culture seedling method and planting equipment thereof |
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