CN103931496A - Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method - Google Patents
Dendrobium flexicaule tissue culture and rapid propagation culture medium as well as tissue culture and rapid propagation method Download PDFInfo
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Abstract
The invention discloses a dendrobium flexicaule tissue culture and rapid propagation culture medium as well as a tissue culture and rapid propagation method. The culture medium consists of magnesium sulfate heptahydrate, monopotassium phosphate, ammonium sulfate, calcium nitrate terahydrate, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, molybdenum trioxide, ferrous sulfate heptahydrate, ethylenediaminetetraacetic acid disodium salt, 6-benzylaminopurine, 1-naphthylacetic acid, agar, sugarcane, white sugar and banana sauce; the pH value of the culture medium is 5.6-5.8; the tissue culture and rapid propagation method comprises steps of sterilization of the culture medium, seed germination and culture, proliferation, rooting culture, seedling hardening and transplanting, and is simple, convenient and easy to implement according to the characteristics of dendrobium flexicaule; the tissue-cultured seedling clusters are strong, free of malformation and deep green in leaf color; the propagation coefficient is increased; the propagation period is about 50 days; the culture effect is good; the survival rate is high.
Description
Technical field
The invention belongs to the tissue culture and rapid propagation method field of plant, particularly a kind of bent stem dendrobe tissue culture fast breeding culture medium and tissue culture and rapid propagation method.
Background technology
The bent stem stem of noble dendrobium, stem is cylindrical, slightly inflection sigmoid, 2～4 pieces, leaf, alternate is in the top of stem, nearly keratin, oval shape lanceolar, is about 3 centimetres, and inflorescence sends from the old stem top of the leaf that fallen, 1～2 flower of tool, the shallow white of petal sheet, ovum shape triangle, is about 3 millimeters, the anxious point of tip; The yellowish green colour band of bennet and ovary is pale purple, the flowers are in blossom exhibition, middle sepal back side yellow green, lavender is slightly with in upper end, Long Circle, petal bottom yellow green, the nearly lavender in top, ellipse, is about 25 millimeters, middle part is wide 13 millimeters, tip is blunt, 5 arteries and veins of tool, May at florescence, produce Henan (Funiu Shan Mountain district), Hubei (Shennongjia), east, Hunan (Southern Mountain, Heng Shan), South Sichuan (Ganluo), be born on the mountain valley rock of 1200～2000 meters of height above sea level.
The stem of noble dendrobium is that the Pharmacopoeia of the People's Republic of China records kind.Belong to conventional traditional Chinese medicine and Chinese patent medicine formula medicine in short supply.Its medical value head is loaded in Shennong's Herbal, classifies as top grade.The stem of noble dendrobium is used as medicine with stem, and there is reinforcing stomach reg fluid, nourishing Yin and clearing heat, pain relieving, hawk, the effect such as improving eyesight, reducing blood lipid, blood pressure, be the primary raw material of producing the products such as " Shiyu Yeguang Wan ", " the fresh dew of the stem of noble dendrobium ", " dendrobium stem beverage ", " capsule with dendrobium stem "." Mailuoning " series of products of exploitation taking the stem of noble dendrobium as primary raw material, are appointed as the indispensable good medicine of national each hospital angiocardiopathy by country, and the stem of noble dendrobium is also widely used in the fields such as food, beauty treatment, health care.The domestic pharmacy corporation taking the stem of noble dendrobium as raw material has family more than 160, more than ten thousand ton of year fresh stem of noble dendrobium l of demand.At present, supply falls short of demand for stem of noble dendrobium product, the development trend that price goes up in being surely year by year.
Record according to Compendium of Material Medica.Stem of noble dendrobium effect " except gas under numbness, tonifying five zang organs consumptive disease, reinforcing yin essence benefit essence, strengthens the bone, warming the kidney, kidney tonifying benefit power, makes light of one's life by commiting suicide and prolongs life." modern medicine study proves, the stem of noble dendrobium has enhancing immunity, and inhibition tumor cell is anti-ageing, and antifatigue is hypoglycemic, stimulates circulation, and strengthens the effects such as cardio-pulmonary function.The Wild Medicinal stem of noble dendrobium is grown nonparasitically upon another plant on large trunk or between cliff crack of stone more.The climatic environment of happiness warm and moist and half cloudy half light.Though it blooms many, result is few, plants carefully as dust, and only 0.3～0.4 microgram, need could germinate with mycosymbiosis.This just become its in wild environment regeneration capacity very a little less than.Because human lives's scope constantly expands.Add long-term excessive harvesting.Its production-goods source has been on the verge of exhaustion.List in " endangered species of wild fauna and flora international trade pact ".
In recent years as medicinal Dendrobium Sw day by day by extensive concern; the Henan stem of noble dendrobium, the bent stem stem of noble dendrobium are put into " Medicinal Dendrobium in China figure will " because medical value is high; list " Henan Province lays special stress on protecting plant register " in; the bent stem stem of noble dendrobium is for many years completely by the wild medicine that supplies; wild resource is more and more exhausted, and natural medicine source is endangered.
Xixia, Henan has advantageous climatic environment condition, is the He Yuan producing region, the genuine place of production of the bent stem stem of noble dendrobium.On implantation methods, local medicinal herb grower adopts traditional approach, excavates and plant division cultivation with field, consumes in a large number bent stem stem of noble dendrobium wild resource, causes germ plasm resource rare, and reproduction coefficient is low, and development speed is slow, and plantation scale cannot effectively expand.And because culture technique is backward, extensive management is simple, causing the survival rate of the bent stem stem of noble dendrobium low, growth cycle is long, yields poorly, of poor benefits.
Summary of the invention
The above-mentioned defect existing for solving prior art, the object of the present invention is to provide a kind of bent stem dendrobe tissue culture fast breeding culture medium.
Another object of the present invention is to provide a kind of bent stem dendrobe tissue culture quick-breeding method, the method is for the feature of the bent stem stem of noble dendrobium and design, and growth rate is fast, and the seedling cycle is short, and tissue-culturing rapid propagation is effective, and survival rate is high.
The technical solution used in the present invention is as follows:
A kind of bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 300～350 milligrams of epsom salts, 250～300 milligrams of potassium dihydrogen phosphates, 500～550 milligrams, ammonium sulfate, 800～1000 milligrams of four water-calcium nitrates, boric acid 0.05-0.15 milligram, four water manganese sulphate 7.0-8.0 milligrams, white vitriol 0.25-0.45 milligram, cupric sulfate pentahydrate 0.04-0.10 milligram, molybdenum trioxide 0.01-0.03 milligram, 28～56 milligrams of ferrous sulfate heptahydrates, disodium ethylene diamine tetraacetate 36-52 milligram, 0.2～3.0 milligram of 6-benzyladenine, 0.1～2.0 milligram of a-methyl α-naphthyl acetate, agar
5.0～5.2 grams, sucrose 15.0
～15.5gram, white granulated sugar 15.0
～15.5gram, 100～110 grams of banana puree, the pH value of described medium is 5.6～5.8.
A kind of bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 300 milligrams of epsom salts, 300 milligrams of potassium dihydrogen phosphates, 550 milligrams, ammonium sulfate, 1000 milligrams of four water-calcium nitrates, 0.10 milligram of boric acid, four 7.5 milligrams of water manganese sulphates, 0.33 milligram of white vitriol, 0.04 milligram of cupric sulfate pentahydrate, 0.016 milligram of molybdenum trioxide, 28 milligrams of ferrous sulfate heptahydrates, 37.3 milligrams of disodium ethylene diamine tetraacetates, 3.0 milligrams of 6-benzyladenines, 2.0 milligrams of a-methyl α-naphthyl acetates, 5.2 grams, agar, 15.0 grams of sucrose, 15.0 grams of white granulated sugars, 100 grams of banana puree, the pH value of described medium is 5.6.
A tissue culture and rapid propagation method for the bent stem stem of noble dendrobium, is characterized in that operating as follows:
(1) sterilizing of medium
Medium claimed in claim 1, at 120～125 DEG C, is carried out to sterilizing 20min under the pressure of 1.06～1.08kg/cm2;
(2) seed germination, the induction of stem section are cultivated with startup
The bent stem stem of noble dendrobium fruit that fresh maturation is not split, taking volumetric concentration is 13 seconds of alcohol sterilization treatment of 75%, mass concentration is that 10% calcium hypochlorite is processed 20-30 minute, be sown into and utilize described in claim 1 in bent stem dendrobe tissue culture fast breeding culture medium, carry out seed germination cultivation and stem section and start cultivation, cultivation temperature 22-24 DEG C, illumination every day 10-12 hour, intensity of illumination 1800-2500Lx, within 15-20 days, embryo starts to sprout, within 25-30 days, embryo expands, and stem segment base portion expands, and forms green Multiple Buds;
(3) propagation, culture of rootage
Sprout after the green Multiple Buds forming is cut and proceed to medium, every 25～30 days, cut apart Multiple Buds, repeated inoculation step is bred cultivation, and cutting apart Multiple Buds can not be too small, and need have 3-4 bud is one clump; Between propagation culture period, carry out dark place reason, described dark place is managed after inoculation and is carried out for the 1st day, and the dark processing time is 7～10 days; Breed after a certain amount of and the tissue culture plant inoculation of robust growth is carried out in medium to culture of rootage, the condition of described propagation, culture of rootage is 22 ± 2 DEG C of temperature, intensity of illumination 2000Lx, and light application time is 12 hours/day;
(4) hardening and transplanting
By the group training seedling of taking root in greenhouse through the half-open glass cap hardening of 1～2 day and the standard-sized sheet glass cap hardening of 3～5 days; Take out group training seedling wherein, clean with clear water the medium that root system adheres to, plant into cave dish, transplanting medium is that little bark, liver moss and handstone volume ratio are 2:3: 2 mixture; 10～15 days of transplanting initial stage, keep 24～27 DEG C of temperature, and relative moisture remains on more than 90%.
Bent stem dendrobe tissue culture fast breeding culture medium provided by the invention and tissue culture and rapid propagation method, for the feature of the bent stem stem of noble dendrobium, simple and easy to do, group training seedling bud clump stalwartness, without deformity, leaf look dark green, and reproduction coefficient improves, and the breeding cycle is short, approximately 50 days, culture effect was good, and survival rate is high.The present invention adopts unique bent stem dendrobe tissue culture seedling production technology and cultivation method, turns out seedlings more than 100,000 strains from a wild bent stem stem of noble dendrobium capsule in locality, has effectively solved the in short supply of seedling and has carried out source problem; Group training seedling white silk seedling, transplanting survival rate reach more than 97%, output improves more than 40% immense success, its advance occupies the prostatitis of domestic and international colleague's research and application, significant to resources of medicinal plant in short supply regeneration in imminent danger and sustainable utilization, make up the blank in domestic and international this field.Successfully solve bent stem stem of noble dendrobium seedling breeding technology, and according to bent stem stem of noble dendrobium place of production suitability optimization principles and its ecotope requirement, in Er Langping town, Xixia County, bent stem stem of noble dendrobium standardized planting and GAP experiment and demonstration base are built in choosing, and field management and maintenance survival rate is high.The inventive method is fostered and pseudo-wild cultivating herb resource protection; promote the sustainable use of herb resource; can be for production of crude drugs enterprise provides complete bent stem Rapid Propagation of Dendrobium and management technique specification, to promoting that bio-diversity conservation has important practical significance.
Embodiment
Embodiment 1
A kind of bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 300 milligrams of epsom salts, 300 milligrams of potassium dihydrogen phosphates, 550 milligrams, ammonium sulfate, 1000 milligrams of four water-calcium nitrates, 0.10 milligram of boric acid, four 7.5 milligrams of water manganese sulphates, 0.33 milligram of white vitriol, 0.04 milligram of cupric sulfate pentahydrate, 0.016 milligram of molybdenum trioxide, 28 milligrams of ferrous sulfate heptahydrates, 37.3 milligrams of disodium ethylene diamine tetraacetates, 3.0 milligrams of 6-benzyladenines, 2.0 milligrams of a-methyl α-naphthyl acetates, 5.2 grams, agar, 15.0 grams of sucrose, 15.0 grams of white granulated sugars, 100 grams of banana puree, the pH value of described medium is 5.6.
Utilize the tissue culture and rapid propagation method of this song stem dendrobe tissue culture fast breeding culture medium to the bent stem stem of noble dendrobium, carry out in the steps below:
It is 8.5 centimetres that the medium preparing is put into height, and bore is the vial of 5.5 centimetres, 50 milliliters every bottle, cover tightly bottle cap, and at 120～125 DEG C, 1.06～1.08kg/cm
2pressure under carry out sterilizing 20min, stand-by;
Bent stem stem of noble dendrobium seedling carries out artificial pollination mid-May, get by the end of November still uncracked fruit of ripe jaundice, first rinse with running water, scrub each gap with liquid detergent again, finally rinse 30 minutes with flowing water, take and on superclean bench, carry out sterilizing, the alcohol sterilizing that volumetric concentration is 75% 13 seconds, be that 10% calcium hypochlorite is processed 10-20 minute, aseptic water washing 3～4 times by mass concentration.
On superclean bench, the fruit after sterilization is cut, seed broadcasting is in aseptic culture medium, put it into that culturing room breeds and culture of rootage, condition of culture is 25 ± 2 DEG C of temperature, cultivating light source is 28W straight pipe type power saving fluorescent lamp, intensity of illumination 2000Lx, light application time is 12 hours/day, within 15-20 days, embryo starts to sprout, within 25-30 days, embryo expands, on medium, protocorm sprouts aseptic Multiple Buds, cutting a Multiple Buds 3-5 bud is one clump, be inoculated in medium, constantly shoot proliferation, between propagation culture period, carry out dark place reason, described dark place is managed after inoculation and is carried out for the 1st day, the dark processing time is 7～10 days, breed after a certain amount of and the tissue culture plant inoculation of robust growth is carried out in medium to culture of rootage, the condition of described propagation, culture of rootage is 22 ± 2 DEG C of temperature, intensity of illumination 2000Lx, and light application time is 12 hours/day.
Rise and emerge annual March, the group training seedling of taking root in greenhouse through the half-open glass cap hardening of 1～2 day and the standard-sized sheet glass cap hardening of 3～5 days, take out group training seedling wherein, clean the medium of root system adhesion with clear water, according to suitability optimization principles and its ecotope requirement, plant into cave bed, open country cool canopy, transplanting medium is that little bark, liver moss and handstone volume ratio are 2:3: 2 mixture, transplant 10～15 days of initial stage, keep 25～28 DEG C of temperature, relative moisture remains on more than 90%.Transplanting survival rate is 92%, hardening 1 year.After 1 year, seedlings are transferred on ophiolite+liver moss and are fixed with line card, press the management of the GAP specifications of quality under suitable temp. and humidity and illumination condition.
Employing the invention provides medium and tissue culture and rapid propagation method, and the newborn blade of bent stem dendrobe tissue culture seedling of turning out is leaf normal, and leaf look dark green, and attitude stretches.Group is trained seedling after acclimatization and transplants, robust growth, and cane enriches, and newborn Miao Qu stem stem of noble dendrobium feature is obvious, vigorous in the growth of original producton location pseudo-wild cultivating, solid various after artificial pollination.
Embodiment 2
A kind of bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 350 milligrams of epsom salts, 250 milligrams of potassium dihydrogen phosphates, 500 milligrams, ammonium sulfate, 800 milligrams of four water-calcium nitrates, 0.15 milligram of boric acid, 7.0 milligrams of four water manganese sulphates, 0.45 milligram of white vitriol, 0.10 milligram of cupric sulfate pentahydrate, 0.03 milligram of molybdenum trioxide, 56 milligrams of ferrous sulfate heptahydrates, 52 milligrams of disodium ethylene diamine tetraacetates, 0.2 milligram of 6-benzyladenine, 0.1 milligram of a-methyl α-naphthyl acetate, agar
5.0gram, sucrose
15.5gram, white granulated sugar
15.5gram, 105 grams of banana puree, the pH value of described medium is 5.8.
Utilize bent stem dendrobe tissue culture fast breeding culture medium to be undertaken numerous soon by the method in embodiment 1 to the tissue culture and rapid propagation method of the bent stem stem of noble dendrobium.
Embodiment 3
A kind of bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 330 milligrams of epsom salts, 270 milligrams of potassium dihydrogen phosphates, 520 milligrams, ammonium sulfate, 900 milligrams of four water-calcium nitrates, 0.05 milligram of boric acid, 8.0 milligrams of four water manganese sulphates, 0.25 milligram of white vitriol, 0.07 milligram of cupric sulfate pentahydrate, 0.01 milligram of molybdenum trioxide, 46 milligrams of ferrous sulfate heptahydrates, 36 milligrams of disodium ethylene diamine tetraacetates, 2.0 milligrams of 6-benzyladenines, 0.6 milligram of a-methyl α-naphthyl acetate, agar
5.1gram, 15.3 grams of sucrose, 15.3 grams of white granulated sugars, 110 grams of banana puree, the pH value of described medium is 5.7.
Utilize bent stem dendrobe tissue culture fast breeding culture medium to be undertaken numerous soon by the method in embodiment 1 to the tissue culture and rapid propagation method of the bent stem stem of noble dendrobium.
Claims (3)
1. a bent stem dendrobe tissue culture fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 300～350 milligrams of epsom salts, 250～300 milligrams of potassium dihydrogen phosphates, 500～550 milligrams, ammonium sulfate, 800～1000 milligrams of four water-calcium nitrates, boric acid 0.05-0.15 milligram, four water manganese sulphate 7.0-8.0 milligrams, white vitriol 0.25-0.45 milligram, cupric sulfate pentahydrate 0.04-0.10 milligram, molybdenum trioxide 0.01-0.03 milligram, 28～56 milligrams of ferrous sulfate heptahydrates, disodium ethylene diamine tetraacetate 36-52 milligram, 0.2～3.0 milligram of 6-benzyladenine, 0.1～2.0 milligram of a-methyl α-naphthyl acetate, agar
5.0～5.2 grams, sucrose 15.0
～15.5gram, white granulated sugar 15.0
～15.5gram, 100～110 grams of banana puree, the pH value of described medium is 5.6～5.8.
2. the bent stem dendrobe tissue culture of one according to claim 1 fast breeding culture medium, it is characterized in that by containing following material in every liter of distilled water formulated: 300 milligrams of epsom salts, 300 milligrams of potassium dihydrogen phosphates, 550 milligrams, ammonium sulfate, 1000 milligrams of four water-calcium nitrates, 0.10 milligram of boric acid, four 7.5 milligrams of water manganese sulphates, 0.33 milligram of white vitriol, 0.04 milligram of cupric sulfate pentahydrate, 0.016 milligram of molybdenum trioxide, 28 milligrams of ferrous sulfate heptahydrates, 37.3 milligrams of disodium ethylene diamine tetraacetates, 3.0 milligrams of 6-benzyladenines, 2.0 milligrams of a-methyl α-naphthyl acetates, 52 grams, agar, 150 grams of sucrose, 150 grams of white granulated sugars, 100 grams of banana puree, the pH value of described medium is 5.6.
3. a tissue culture and rapid propagation method for the bent stem stem of noble dendrobium, is characterized in that operating as follows:
(1) sterilizing of medium
By medium claimed in claim 1 at 120～125 DEG C, 1.06～1.08kg/cm
2pressure under carry out sterilizing 20min;
(2) seed germination, the induction of stem section are cultivated with startup
The bent stem stem of noble dendrobium fruit that fresh maturation is not split, taking volumetric concentration is 13 seconds of alcohol sterilization treatment of 75%, mass concentration is that 10% calcium hypochlorite is processed 20-30 minute, be sown into and utilize described in claim 1 in bent stem dendrobe tissue culture fast breeding culture medium, carry out seed germination cultivation and stem section and start cultivation, cultivation temperature 22-24 DEG C, illumination every day 10-12 hour, intensity of illumination 1800-2500Lx, within 15-20 days, embryo starts to sprout, within 25-30 days, embryo expands, and stem segment base portion expands, and forms green Multiple Buds;
(3) propagation, culture of rootage
Sprout after the green Multiple Buds forming is cut and proceed to medium, every 25～30 days, cut apart Multiple Buds, repeated inoculation step is bred cultivation, and cutting apart Multiple Buds can not be too small, and need have 3-4 bud is one clump; Between propagation culture period, carry out dark place reason, described dark place is managed after inoculation and is carried out for the 1st day, and the dark processing time is 7～10 days; Breed after a certain amount of and the tissue culture plant inoculation of robust growth is carried out in medium to culture of rootage, the condition of described propagation, culture of rootage is 22 ± 2 DEG C of temperature, intensity of illumination 2000Lx, and light application time is 12 hours/day;
(4) hardening and transplanting
By the group training seedling of taking root in greenhouse through the half-open glass cap hardening of 1～2 day and the standard-sized sheet glass cap hardening of 3～5 days; Take out group training seedling wherein, clean with clear water the medium that root system adheres to, plant into cave dish, transplanting medium is that little bark, liver moss and handstone volume ratio are 2:3: 2 mixture; 10～15 days of transplanting initial stage, keep 24～27 DEG C of temperature, and relative moisture remains on more than 90%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542287A (en) * | 2014-12-31 | 2015-04-29 | 安徽康久生物科技有限公司 | Preparation method of special tissue culture solution for dendrobium candidum |
| CN107360969A (en) * | 2017-07-26 | 2017-11-21 | 河南联源生物科技有限公司 | Culture medium and method suitable for tissue culture seedling raising of wild dendrobium candidum in Funiu mountain of Henan province |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| CN110100737A (en) * | 2019-06-21 | 2019-08-09 | 台州市椒江草之堂生物科技有限公司 | A kind of candidum tissue culturing rapid propagation method |
| CN110574686A (en) * | 2019-10-24 | 2019-12-17 | 永州职业技术学院 | In-vitro cultivation method of Dendrobium Nanyue |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548681A (en) * | 2013-10-31 | 2014-02-05 | 浙江农林大学天目学院 | Culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile |
-
2014
- 2014-04-02 CN CN201410129938.3A patent/CN103931496B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548681A (en) * | 2013-10-31 | 2014-02-05 | 浙江农林大学天目学院 | Culture medium for rapidly inducing adventitious buds in tissue culture of dendrobium nobile |
Non-Patent Citations (3)
| Title |
|---|
| 余晓丽等: ""野生曲茎石斛的离体培养及再生体系研究"", 《武汉工业学院学报》 * |
| 杨联河等: ""曲茎石斛快速繁殖研究"", 《中国中药杂志》 * |
| 高立献等: ""石斛无菌播种试管育苗试验简报"", 《河南林业科技》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542287A (en) * | 2014-12-31 | 2015-04-29 | 安徽康久生物科技有限公司 | Preparation method of special tissue culture solution for dendrobium candidum |
| CN104542287B (en) * | 2014-12-31 | 2017-11-21 | 蒙城县望槐信息科技有限责任公司 | A kind of preparation method of dendrobium candidum private organizations nutrient solution |
| CN107360969A (en) * | 2017-07-26 | 2017-11-21 | 河南联源生物科技有限公司 | Culture medium and method suitable for tissue culture seedling raising of wild dendrobium candidum in Funiu mountain of Henan province |
| CN107556119A (en) * | 2017-10-24 | 2018-01-09 | 山西梅芝园艺有限公司 | A kind of special nutrient fluid suitable for bottle plant thing |
| CN110100737A (en) * | 2019-06-21 | 2019-08-09 | 台州市椒江草之堂生物科技有限公司 | A kind of candidum tissue culturing rapid propagation method |
| CN110574686A (en) * | 2019-10-24 | 2019-12-17 | 永州职业技术学院 | In-vitro cultivation method of Dendrobium Nanyue |
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