CN107041302A - A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting - Google Patents
A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting Download PDFInfo
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- CN107041302A CN107041302A CN201710017188.4A CN201710017188A CN107041302A CN 107041302 A CN107041302 A CN 107041302A CN 201710017188 A CN201710017188 A CN 201710017188A CN 107041302 A CN107041302 A CN 107041302A
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- China
- Prior art keywords
- culture medium
- dendrobium
- aseptic seeding
- fruit pod
- sprouting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of dendrobium aseptic seeding and the culture medium of sprouting and rooting, culture medium is：MS+NAA0.1mg/L+6 BA2.0mg/L+ banana 10g/L+ mashed potatoes 100g/L+ sucrose 30g/L+ activated carbon 1g/L+ agar 4g/L, pH=5.7.The dendrobium in ripe Fruit pod period is taken to be cultivated：68 months after pollination, Fruit pod does not ftracture, when Fruit pod carpopodium position starts to turn yellow.24 to 26 DEG C of cultivation temperature, intensity of illumination is 60 μm of olm‑2s‑1, hour/day of light application time 12.Aseptic seeding germination percentage is high；Different kinds of culture medium need not be changed to the seedling stage by being sprouted from aseptic seeding；From starting, aseptic seeding is shorter to the strong sprout bottle outlet cycle.
Description
Technical field
The present invention relates to the culture of a kind of culture medium of flowers, more particularly to a kind of dendrobium aseptic seeding and sprouting and rooting
Base.
Background technology
Orchid is one of big famous flower of China ten, is also one of world's rare flower, occupies particularly significant in the production of flowers and plants
Status.With it is economical develop rapidly, the quickening and the improvement of people's living standards of urbanization process, traditional orchid is
The growing aesthetic requirement of people can not be met, as the dendrobium of one of " four big cattleyas ", because cultivation is easy, easily into
Flower, pattern is very gorgeous, and flower-shape is changeable, and the florescence is permanent, and various colors are colorful, and the favorite of market flowers is had become at present.
A large amount of seedling can be obtained by aseptic seeding, be the Main Means that new varieties are cultivated in crossbreeding.But at present
There is following defect through disclosed culture medium.
(1) aseptic seeding germination percentage is low；
(2) being sprouted from aseptic seeding to the seedling stage needs to change different kinds of culture medium, if desired for root media,
Strong seedling culture base etc.；
(3) it is long to the strong sprout bottle outlet cycle from aseptic seeding is started, general cycle 10-12 months or so.
The content of the invention
It is an object of the invention to provide a kind of dendrobium aseptic seeding and the culture medium of sprouting and rooting.
The purpose of the present invention is achieved through the following technical solutions：
The component of following amount is included in the dendrobium aseptic seeding of the present invention and the culture medium of sprouting and rooting, every L culture mediums：
NAA0.1mg+6-BA2.0mg+ banana 10g+ mashed potatoes 100g+ sucrose 30g+ activated carbon 1g+ agar 4g, remaining group
Part is MS, the pH=5.7 of culture medium.
As seen from the above technical solution provided by the invention, dendrobium aseptic seeding provided in an embodiment of the present invention and
The culture medium of sprouting and rooting, aseptic seeding germination percentage is high；Different trainings need not be changed to the seedling stage by being sprouted from aseptic seeding
Support base species；From starting, aseptic seeding is shorter to the strong sprout bottle outlet cycle.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on embodiments of the invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to protection scope of the present invention.
The dendrobium aseptic seeding of the present invention and the culture medium of sprouting and rooting, its preferably embodiment is：
The component of following amount is included in per L culture mediums：
NAA0.1mg+6-BA2.0mg+ banana 10g+ mashed potatoes 100g+ sucrose 30g+ activated carbon 1g+ agar 4g, remaining group
Part is MS, the pH=5.7 of culture medium.
The dendrobium in ripe Fruit pod period is taken to be cultivated, the ripe Fruit pod period refers to：6-8 months, fruit after pollination
Pod is not ftractureed, when Fruit pod carpopodium position starts to turn yellow.
24 to 26 DEG C of cultivation temperature, intensity of illumination is 60 μm of olm-2s-1, hour/day of light application time 12.
The dendrobium aseptic seeding of the present invention and the culture medium of sprouting and rooting, aseptic seeding germination percentage are high；From aseptic seeding
Different kinds of culture medium need not be changed by sprouting the seedling stage；From starting, aseptic seeding is shorter to the strong sprout bottle outlet cycle.
Specific embodiment：
Ripe Fruit pod picking time：6-8 months after pollination, Fruit pod does not ftracture, when Fruit pod carpopodium position starts to turn yellow.
Culture medium is：MS+NAA0.1mg/L+6-BA2.0mg/L+ banana 10g/L+ mashed potatoes 100g/L+ sucrose 30g/L+
Activated carbon 1g/L+ agar 4g/L, pH=5.7.
Specifically include step：
(1) for aseptic seeding Fruit pod with it is just ripe but when not yet ftractureing effect preferably, 6-8 after generally pollinating
Month, when Fruit pod carpopodium position starts to turn yellow preferably.At this moment, seed can be separated from each other in capsule, easily sowing.Immature seed
All it is sticked together during sowing, is unfavorable for late growing stage.Uncracked Fruit pod sterilizing is easy in addition, and only Fruit pod epidermis can be gone out
Bacterium；After Fruit pod cracking, powdered Seed sterilization is more difficult, and Seed germinating ability is reduced.Preferably it is to adopt to broadcast after Fruit pod harvesting, not
It is in time for sowing time, can be installed with sealed polybag and be put into preservation in 4 DEG C of refrigerator, but the time should not be otherwise easily long too long
Mould or reduction germination percentage.
(2) aseptic seeding must be carried out on superclean bench, and all medicaments and instrument are got out before operation, be kept
The germ-free condition of superclean bench.
(3) uncracked pod is removed into persitent perianth, stamen post, carpopodium, after being cleaned through running water, is placed in 10% hypochlorous acid
Sterilized 20 minutes in sodium solution, with 75% alcohol surface sterilization 3 minutes, calcination removes pod surface wine directly on alcolhol burner
Essence, can thus complete sterilization.
(4) Fruit pod is splitted, seed is uniformly sprinkling upon media surface.
(5) dendrobium seed sprouts very fast in 25 DEG C or so germination and growths, the too high easy Necrosis of temperature, the too low hair of temperature
Bud is slow.When temperature is less than 10 DEG C, even if meeting nutritional condition can not sprout.Intensity of illumination is 60 μm of olm-2s-1, illumination
Time 12 hour/day.
(6) protocorm can be formed after sowing 2 weeks, first leaf can be born within 4-6 weeks.Now carry out first time sub-bottle.12-14
Zhou Hou, after growing 2-3 pieces leaf and taking root, can carry out second of sub-bottle.If middle seedling growth is overstocked to carry out third time
Sub-bottle.After 22-24 weeks when dendrobium seedling grows to 10~15cm, there are 2~3 preferable roots, bottle outlet transplanting can be carried out.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art is in the technical scope of present disclosure, the change or replacement that can be readily occurred in,
It should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Enclose and be defined.
Claims (3)
1. the culture medium of a kind of dendrobium aseptic seeding and sprouting and rooting, it is characterised in that include following amount in per L culture mediums
Component：
NAA0.1mg+6-BA2.0mg+ banana 10g+ mashed potatoes 100g+ sucrose 30g+ activated carbon 1g+ agar 4g, remaining component is
MS, the pH=5.7 of culture medium.
2. the culture medium of dendrobium aseptic seeding according to claim 1 and sprouting and rooting, it is characterised in that take maturation
The dendrobium in Fruit pod period is cultivated, and the ripe Fruit pod period refers to：6-8 months after pollination, Fruit pod does not ftracture, Fruit pod carpopodium
When position starts to turn yellow.
3. the culture medium of dendrobium aseptic seeding according to claim 2 and sprouting and rooting, it is characterised in that cultivation temperature
24 to 26 DEG C, intensity of illumination is 60 μm of olm-2s-1, hour/day of light application time 12.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710017188.4A CN107041302A (en) | 2017-01-10 | 2017-01-10 | A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710017188.4A CN107041302A (en) | 2017-01-10 | 2017-01-10 | A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting |
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| Publication Number | Publication Date |
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| CN107041302A true CN107041302A (en) | 2017-08-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710017188.4A Pending CN107041302A (en) | 2017-01-10 | 2017-01-10 | A kind of culture medium of dendrobium aseptic seeding and sprouting and rooting |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328985A (en) * | 2018-11-22 | 2019-02-15 | 安徽祥峰生物科技有限公司 | It is a kind of promote Dendrobidium huoshanness seed sprout culture medium and type of seeding |
| CN115700074A (en) * | 2022-10-25 | 2023-02-07 | 海南大学 | Disposable seedling culture medium and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102499092A (en) * | 2011-11-17 | 2012-06-20 | 云南金九地生物科技有限公司 | Method for cultivating and breeding cell tissue of tissue culture seedling of Dendrobium candidum |
| CN104938331A (en) * | 2015-05-27 | 2015-09-30 | 广西健宝石斛有限责任公司 | Tissue culture propagation method of dendrobium officinale |
| CN105052753A (en) * | 2015-09-29 | 2015-11-18 | 江苏农林职业技术学院 | Rapid propagation method for dendrobii officmalis caulis |
| CN106804425A (en) * | 2016-12-23 | 2017-06-09 | 中国林业科学研究院林业研究所 | A kind of cultural method of Bowring cattleya high-efficiency tissue culture |
-
2017
- 2017-01-10 CN CN201710017188.4A patent/CN107041302A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499092A (en) * | 2011-11-17 | 2012-06-20 | 云南金九地生物科技有限公司 | Method for cultivating and breeding cell tissue of tissue culture seedling of Dendrobium candidum |
| CN104938331A (en) * | 2015-05-27 | 2015-09-30 | 广西健宝石斛有限责任公司 | Tissue culture propagation method of dendrobium officinale |
| CN105052753A (en) * | 2015-09-29 | 2015-11-18 | 江苏农林职业技术学院 | Rapid propagation method for dendrobii officmalis caulis |
| CN106804425A (en) * | 2016-12-23 | 2017-06-09 | 中国林业科学研究院林业研究所 | A kind of cultural method of Bowring cattleya high-efficiency tissue culture |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328985A (en) * | 2018-11-22 | 2019-02-15 | 安徽祥峰生物科技有限公司 | It is a kind of promote Dendrobidium huoshanness seed sprout culture medium and type of seeding |
| CN115700074A (en) * | 2022-10-25 | 2023-02-07 | 海南大学 | Disposable seedling culture medium and application thereof |
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Application publication date: 20170815 |