CN102283110A - Method for rapidly propagating moth orchids through inducing cluster buds by leaves - Google Patents
Method for rapidly propagating moth orchids through inducing cluster buds by leaves Download PDFInfo
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- CN102283110A CN102283110A CN 201010296193 CN201010296193A CN102283110A CN 102283110 A CN102283110 A CN 102283110A CN 201010296193 CN201010296193 CN 201010296193 CN 201010296193 A CN201010296193 A CN 201010296193A CN 102283110 A CN102283110 A CN 102283110A
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Abstract
The invention discloses a method for rapidly propagating moth orchids through inducing cluster buds by leaves, particularly a method comprising the following steps of: disinfecting and culturing moth orchid peduncle buds to obtain sterile materials; removing leaves of the sterile materials and germinating the sterile peduncle buds to obtain the cluster buds; propagating the cluster buds while utilizing the leaves of the sterile materials and/or the cluster buds to induce the cluster buds; and finally rapidly propagating the moth orchids by taking the cluster buds as culturing seedlings of a propagating object culturing group. The method provided by the invention has the advantages of fast propagating speed, short growth cycle and reduced production cost.
Description
Technical field
The present invention relates to a kind of method, the invention belongs to vegetative tissue culture method by the blade propagating quickly butterfly orchid.
Background technology
Moth orchid is the system famous flower, with its form graceful unique, flower is very large, pattern is gorgeous, color and luster is abundant, the florescence is long, be called as " after the orchid ", and be the single shaft stem flowers that grow nonparasitically upon another plant, plant seldom sprouts the lateral bud branch, conventional propagation method is difficult to meet the need of market, and tissue culture is the effective way of propagating quickly butterfly orchid.Nineteen sixty, Morel has proposed the method for isolated vegetative propagation orchid.Along with the popularization of Moth orchid, the research of the fast traditional font of Moth orchid system is also goed deep into gradually.Utilize the mellow fruit pod to carry out aseptic seeding, obtain tree seedling; Utilize the aseptic seeding seedling to induce and intend the protocorm or the bud of growing thickly; Explant carries out disinfection, and bud or plan protocorm obtain to grow thickly; Also have to cultivate by aseptic blade dedifferentiation to obtain protocorm, the further proliferation and differentiation of the protocorm bud that becomes to grow thickly is bred.The cultivation aspect of sprouting by the direct induced bundle of blade is not seen detailed report as yet at present.
Although the Moth orchid fast breeding technique is obtained certain progress, Moth orchid is grown thickly, and the bud reproduction coefficient is low, the plan protocorm need break up special culture media and intend protocorm dissociation rate height, and these all are restriction orchid in factory factors of production.
Summary of the invention
At the problems referred to above, the invention provides a kind of new method of propagating quickly butterfly orchid of sprouting by the blade induced bundle, this method has the advantage that reproduction speed is fast, growth cycle short, reduce production costs, and produces significant for the Moth orchid industrialization.
Method of the present invention is earlier to carry out disinfection, cultivate and obtain sterilizable material with Moth orchid bennet bud, the sterilizable material defoliation, obtain to grow thickly bud with aseptic bennet bud sprouting, when the bud of growing thickly is bred, utilize blade such as aseptic blade discarded in the conventional breeding and the bud-leaf sheet of growing thickly to carry out induced bundle and sprouts, blade carries out with the bud breeding subculture of growing thickly, and promptly the grow thickly blade of bud can both utilize again per generation, induce the new bud of growing thickly, cultivating with the bud of growing thickly at last is tissue cultivating seedling.
Obtain with Moth orchid bennet bud in the step of sterilizable material, Moth orchid bennet bud carries out wiping through 75% ethanol cotton balls, peel off bennet bud bract, the bud section that exposes the bennet bud was soaked 10-15 minute through 0.1% mercuric chloride, wash the back repeatedly with sterile water and insert inducing culture: 1/3MS+BA2-3mg/L+NAA0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.Cultivation through 45 days-60 days obtains aseptic seedling.
Aseptic seedling carries out in fast numerous process, aseptic blade is cut, the square of the about 1cm of diameter, the edge scratches with blade, and blade is tiled in the special culture media that induced bundle is sprouted: spend precious No. 1 2g/L+BA2-5mg/L+NAA0.1-0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Simultaneously, utilize the budlet of removing blade, carry out conventional inducing clumping bud 1/3MS+BA2-3mg/L+NAA0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.Through 45 days-60 days cultivation, obtain to grow thickly bud.In inducing process, blade is sprouted all around and to grow thickly bud, and maximum blades differentiate 13 buds of growing thickly.
The bud of growing thickly of inducing with blade, and with the bud of growing thickly that budlet is induced finish propagation, strong sprout, in the step of taking root, in the repeatedly subculture process of propagation, the bud of growing thickly that blade and budlet constantly induce differentiation to make new advances.Usually after finishing 10 shoot proliferations, the bud of will growing thickly carries out strong sprout, changes root media subsequently over to.
In this method, used medium is described below: MS is the minimal medium prescription, and 1/3MS represents that the consumption of macroelement is 1/3 of a positive usual amounts, and compositions such as other trace are constant; BA, NAA is plant hormone commonly used; Peptone is a beef peptone; Sucus Cocois is the liquid in the coconut; Spending precious No. 1 is import fertilizer, purchases in Hong Kong.
The phenomenon that the blade induced bundle is sprouted is specifically seen picture.About inductivity, statistics shows that handled 3 kinds all can induce the bud of growing thickly by blade.The ratio 30-70% that the blade induced bundle of different cultivars is sprouted.The quantity that the blade induced bundle of an about 1cm of diameter is sprouted is up to 13 buds, average 3 buds.
The present invention is sprouted by the blade induced bundle, reaches the purpose of propagating quickly butterfly orchid, and compared with prior art, this method has the following advantages.
(1) traditional method is to induce the plan protocorm by other organs such as blades, and this approach need be through intending protocorm differential period again, and step increases, and expends cost, and aberration rate is higher in the protocorm atomization.This invention advantage be to utilize Moth orchid conventional fast numerous in discarded blade, in same medium, directly induce the bud of growing thickly, can obtain the bud of growing thickly in a large number by this approach, reduced cost, shortened the cultivation period of new varieties widely, introduced to the market fast for new varieties and have breakthrough technological innovation.
The strain blade on average can downcut 4 blades at least, and a blade on average induces 3 buds, and the bud number that blade is induced is 12 buds.Consider and induce success rate 30-70%,, can induce 3.6 buds by 30% accounting.Be that appreciation rate has turned over nearly 3 times.Utilize the blade induced bundle application prospect of sprouting considerable, need for the new varieties of preciousness that short-term is fast numerous to go out a large amount of clone's seedlings, use fast numerous time that this method can effectively shorten Moth orchid, in the batch production of Moth orchid is produced, very big using value is arranged.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to scope of the present invention.
If do not specialize the conventional means that used technological means is well known to those skilled in the art among the embodiment.
Embodiment 1
No. 1 safflower Moth orchid of boat butterfly bennet bud carries out wiping through 75% ethanol cotton balls, peel off bennet bud bract, the bud section that exposes the bennet bud was soaked 12 minutes through 0.1% mercuric chloride, with inserting inducing culture behind the aseptic water washing: 1/3MS+BA3mg/L+NAA0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.Through 48 days cultivation, obtain aseptic seedling.
Utilize aseptic blade to carry out segment, be cut to the square of the about 1cm of diameter, blade edge scratches with blade, and 14 blades are tiled in the special culture media that induced bundle is sprouted: spend precious No. 1+BA5mg/L+NAA0.2mg/L++ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.Through 56 days cultivation, the blade edge bud of growing thickly.In inducing process, blade is sprouted all around and to grow thickly bud, amounts to and obtains 32 buds of growing thickly.
Claims (5)
1. the method by the blade propagating quickly butterfly orchid is characterized in that, utilizes the blade induced bundle to sprout, and cultivates the clonal tissue culture seedling with the bud of growing thickly as the breeding object at last.
2. a kind of method by the blade propagating quickly butterfly orchid according to claim 1 is characterized in that described blade is to obtain by following steps:
Step 1 is got Moth orchid bennet bud and is carried out disinfection, cultivates and obtain sterilizable material;
Step 2, the sterilizable material defoliation obtains to grow thickly bud with aseptic bennet bud sprouting;
Step 3, the bud tissue of growing thickly is bred, cut expand numerous;
Step 4, cut sterilizable material or/and the bud-leaf sheet of growing thickly as new breeding raw material.
3. a kind of method according to claim 2 by the blade propagating quickly butterfly orchid, it is characterized in that, in the described step 1, Moth orchid bennet bud carries out wiping through 75% ethanol cotton balls, peel off bennet bud bract, the bud section that exposes the bennet bud was soaked 10-15 minute through 0.1% mercuric chloride, with inserting inducing culture behind the aseptic water washing: 1/3MS+BA2-3mg/L+NAA0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.Condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.Cultivation through 45 days-60 days obtains aseptic seedling.
4. according to the arbitrary described a kind of method of claim 1-3 by the blade propagating quickly butterfly orchid, it is characterized in that, sprout in the process at the described Phalaenopsis leaves induced bundle that utilizes, under the aseptic condition, blade is cut into the square of the about 1cm of diameter, blade edge scratches with blade, and blade is tiled in the special culture media that induced bundle is sprouted: spend precious No. 1 2g/L+BA2-5mg/L+NAA0.1-0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L.
5. a kind of method according to claim 4 by the blade propagating quickly butterfly orchid, it is characterized in that, sprout in the process at the described Phalaenopsis leaves induced bundle that utilizes, simultaneously, utilize the budlet of removing blade, carry out conventional inducing clumping bud 1/3MS+BA2-3mg/L+NAA0.2mg/L+ peptone 2g/L+ Sucus Cocois 100ml/L+ sucrose 20g/L, condition of culture comprises 25 ± 2 ℃ of temperature, light application time 10-12 hour/day, intensity of illumination 1200-1500Lx.
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| CN 201010296193 CN102283110A (en) | 2010-09-29 | 2010-09-29 | Method for rapidly propagating moth orchids through inducing cluster buds by leaves |
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| CN 201010296193 CN102283110A (en) | 2010-09-29 | 2010-09-29 | Method for rapidly propagating moth orchids through inducing cluster buds by leaves |
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| CN102283110A true CN102283110A (en) | 2011-12-21 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN103004603A (en) * | 2012-12-27 | 2013-04-03 | 福建农林大学 | Plant regeneration method for butterfly orchid |
| CN103755461A (en) * | 2013-12-23 | 2014-04-30 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for culturing orchid tissue and culture method thereof |
| CN103988780A (en) * | 2014-05-23 | 2014-08-20 | 中国长江三峡集团公司 | Method for directly inducing adventitious buds of butterfly orchid |
| CN105941161A (en) * | 2016-06-25 | 2016-09-21 | 合肥隆扬农业科技有限公司 | Tissue culture method of phalaenopsis |
| CN106489461A (en) * | 2015-09-07 | 2017-03-15 | 湖南瑞利农业发展有限公司 | A kind of breeding method of iris bennet Seedling |
-
2010
- 2010-09-29 CN CN 201010296193 patent/CN102283110A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 《农业科技与信息》 20071231 宁书祥等 蝴蝶兰的组织培养快速繁殖技术 32-33 3-5 , 第7期 * |
| 《园艺学报》 20041231 李军等 蝴蝶兰组培工厂化生产技术 413-414 1-5 第31卷, 第3期 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN103004603A (en) * | 2012-12-27 | 2013-04-03 | 福建农林大学 | Plant regeneration method for butterfly orchid |
| CN103755461A (en) * | 2013-12-23 | 2014-04-30 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for culturing orchid tissue and culture method thereof |
| CN103755461B (en) * | 2013-12-23 | 2015-09-16 | 佛山市顺德区今日景艺生物科技有限公司 | The medium that Orchid Tissue is cultivated and cultural method thereof |
| CN103988780A (en) * | 2014-05-23 | 2014-08-20 | 中国长江三峡集团公司 | Method for directly inducing adventitious buds of butterfly orchid |
| CN103988780B (en) * | 2014-05-23 | 2016-04-27 | 中国长江三峡集团公司 | A kind of method of direct induction Moth orchid indefinite bud |
| CN106489461A (en) * | 2015-09-07 | 2017-03-15 | 湖南瑞利农业发展有限公司 | A kind of breeding method of iris bennet Seedling |
| CN105941161A (en) * | 2016-06-25 | 2016-09-21 | 合肥隆扬农业科技有限公司 | Tissue culture method of phalaenopsis |
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Application publication date: 20111221 |