CN111587784A - Rapid propagation method of hydrangea macrophylla - Google Patents
Rapid propagation method of hydrangea macrophylla Download PDFInfo
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- CN111587784A CN111587784A CN202010061211.1A CN202010061211A CN111587784A CN 111587784 A CN111587784 A CN 111587784A CN 202010061211 A CN202010061211 A CN 202010061211A CN 111587784 A CN111587784 A CN 111587784A
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- hydrangea
- axillary buds
- culture medium
- rapid propagation
- inoculating
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention belongs to the technical field of forestry asexual propagation, and particularly relates to a quick propagation method of hydrangea, which comprises the following steps: (1) taking the stem segment of hydrangea as an explant, and carrying out sterile disinfection treatment; (2) cutting the hydrangea stem segments treated in the step (1) into hydrangea stem segments with at least one axillary bud, inoculating the hydrangea stem segments into a culture medium, and germinating the axillary buds; (3) when the axillary buds in the step (2) extend to a certain length, cutting off and inoculating the axillary buds into a culture medium to proliferate the axillary buds to obtain a large number of axillary buds; (4) inoculating the axillary buds obtained in the step (3) to a culture medium for induction culture to form rooted seedlings; (5) transplanting the tissue culture seedling in the step (4), namely the rooted seedling, into a substrate for culturing to obtain the hydrangea aseptic seedling with strong roots and stems. The invention has the advantages that: has the advantages of high propagation speed and high propagation coefficient, and is not limited by seasons and propagation materials.
Description
Technical Field
The invention belongs to the technical field of forestry asexual propagation, and particularly relates to a rapid propagation method of hydrangea macrophylla.
Background
Hydrangea (Hydrangea macrophylla) also known as Hydrangea and Aster ageratoides is a deciduous shrub of the genus Hydrangea of the family Saxifragaceae. The small branches are thick, the leaves are large and thick, the leaves are opposite, the edges of the leaves are serrated, the flowers are large and beautiful, the flowers are white at the beginning, the flower color turns pink or blue gradually along with the change of the pH value of soil at the later stage, and the hydrangea is warm and moist and not drought-resistant.
The hydrangea is originally produced in Sichuan and Japan, is used for gardens in the south of the Yangtze river in the early, bright and clear periods of cultivating the hydrangea in China, and is popular in the fresh cut flower market at present due to the bright and beautiful colors and longer flowering period of the hydrangea in recent years and wide application in landscape greening and gardening design in various parts of China. The method for breeding the hydrangea macrophylla in the original place mainly comprises cuttage, layering, division and the like, although the methods are easy to survive, the breeding efficiency is low, the large demand of the market cannot be met, meanwhile, the hydrangea macrophylla mostly does not breed flowers, and the plants are difficult to obtain through the traditional seed breeding mode.
Disclosure of Invention
The invention aims to provide a quick propagation method of hydrangea, which takes hydrangea stem segments as explants, and utilizes MS culture medium and biological growth regulators such as 6-BA, KT, IBA, GA and the like with different concentrations to induce axillary buds to proliferate, induce root system to form and harden seedlings so as to realize the quick propagation of hydrangea.
The purpose of the invention is realized by the following technical scheme:
a quick propagation method of hydrangea macrophylla is characterized in that: the method comprises the following steps:
(1) taking the stem segment of hydrangea as an explant, and carrying out sterile disinfection treatment;
(2) cutting the hydrangea stem segments treated in the step (1) into hydrangea stem segments with at least one axillary bud, inoculating the hydrangea stem segments into a culture medium, and germinating the axillary buds;
(3) when the axillary buds in the step (2) extend to a certain length, cutting off and inoculating the axillary buds into a culture medium to proliferate the axillary buds to obtain a large number of axillary buds;
(4) inoculating the axillary buds obtained in the step (3) to a culture medium for induction culture to form rooted seedlings;
(5) transplanting the tissue culture seedling in the step (4), namely the rooted seedling, into a substrate for culturing to obtain the hydrangea aseptic seedling with strong roots and stems.
The components of the culture medium in the step (2) are as follows: MS 1.5-2.0mg/L, 6-BA1.5-2.0mg/L, KT 0-0.5mg/L, IBA 0.3.3-0.5 mg/L, sucrose 30g/L and agar 6g/L, and pH is controlled at 5.8-6.0.
The axillary buds are germinated by using the culture medium under the conditions that the temperature is 25 +/-1 ℃, the illumination intensity is 2500Lx and the illumination time is 12 h/d.
The components of the culture medium in the step (3) are as follows: MS 1.5-2.0mg/L, 6-BA1.5-2.0mg/L, GA 0-0.5mg/L, IBA 0.1.1-0.3 mg/L, sucrose 30g/L and agar 6g/L, and pH is controlled at 5.8-6.0.
The culture medium is used for carrying out the proliferation of axillary buds under the conditions of 25 +/-1 ℃, the illumination intensity of 2500Lx and the illumination time of 12 h/d.
The components of the culture medium in the step (4) are as follows: 1/2 MS 0.1-0.3mg/L, IBA 0.1-0.3mg/L, sucrose 30g/L, agar 6g/L, pH controlled at 5.8-6.0.
The culture medium is used for inducing the axillary buds to root under the conditions of the temperature of 23 +/-2 ℃, the illumination intensity of 1800Lx and the illumination time of 12 h/d.
The matrix in the step (5) comprises the following components: the ratio of the turfy soil to the vermiculite to the perlite is 1:1: 1.
The substrate is used for culturing under the conditions of the temperature of 23 +/-2 ℃, the illumination intensity of 1800Lx and the illumination time of 12 h/d.
And (3) cutting off and inoculating after the axillary buds in the step (2) stretch to 2-3 cm.
The invention has the advantages that: has the advantages of high propagation speed and high propagation coefficient, is not limited by seasons and propagation materials, and can obtain a large number of plants in a short time; the method can propagate the hydrangea in great quantity in an artificial control environment, provide a great amount of seedlings for northern potted flowers, fresh cut flowers and seasonal greening markets, reduce the cost of flower merchants, and provide theoretical and technical support for further research of the hydrangea.
Detailed Description
The features of the present invention and other related features are further described in detail below by way of examples to facilitate understanding by those skilled in the art:
example (b): the method for quickly propagating hydrangea in the embodiment uses hydrangea stem segments as explants, utilizes MS culture medium and biological growth regulators such as 6-BA, KT, IBA and GA with different concentrations to induce axillary buds, induce the axillary buds to proliferate, induce the root system to form and harden seedlings so as to realize the quick propagation of hydrangea, and specifically comprises the following steps:
(1) taking annual stem segments of hydrangea as explants, and removing the dirt on the surfaces of the selected stem segments by using a soft brush with a detergent solution before disinfection. Cutting the stem into about 6cm, placing into a beaker, adding two drops of Tween 2.0, covering the opening of the beaker with gauze, and washing with running water for 30 min. After draining, the explant is washed by sterile water for 3 times on a super clean workbench, each time is washed for 1min, then the explant is soaked in 75% alcohol for 30s, the explant is washed by the sterile water for 3 times, then the explant is soaked by 0.1% mercuric chloride for 7min, the bottle is continuously stirred in the soaking process, and then the explant is washed by the sterile water for 3-5 times, so that the explant is sterilized aseptically.
(2) Cutting the stem sections treated in the step (1) into stem sections with 1-2 axillary buds, and inoculating the stem sections in the following culture media: MS +2.0 mg/L, 6-BA +0.5 mg/L, KT +0.5 mg/L, IBA sucrose 30g/L and agar 6g/L, wherein the pH is controlled to be 5.8-6.0, the temperature is 25 +/-1 ℃, the illumination intensity is 2500Lx, the illumination time is 12h/d, and axillary buds begin to germinate after being cultured for 5 d.
(3) When the axillary bud is elongated to 2-3cm in step (2), the cut is inoculated in the following medium: MS +2.0 mg/L, 6-BA +0.5 mg/L, GA +0.2 mg/L, IBA + sucrose 30g/L and agar 6g/L, wherein the pH is controlled to be 5.8-6.0, the temperature is 25 +/-1 ℃, the illumination intensity is 2500Lx, and the illumination time is 12h/d, axillary buds are proliferated in the culture process, and a large amount of axillary buds can be obtained after 30 d.
(4) Inoculating the axillary buds in the step (3) into the following culture medium: 1/2 MS +0.3mg/L, IBA + 30g/L sucrose and 6g/L agar, and the pH is controlled to be 5.8-6.0, the temperature is 23 +/-2 ℃, the illumination intensity is 1800Lx, and the illumination time is 12 h/d.
(5) Transplanting the tissue culture seedlings, namely the rooting seedlings in the step (4) into the following substrates: turfy soil: vermiculite perlite =1:1:1, the temperature is 23 +/-2 ℃, the illumination intensity is 1800Lx, and the illumination time is 12h/d, and after the cultivation is carried out for 30d, the sterile hydrangea macrophylla seedling with strong roots and stems can be grown.
In the fast propagation method of hydrangea in the embodiment, the induction rate of the cluster buds is 85.83%, the multiplication coefficient is 11.92, the rooting rate is 92.16%, and the seedling hardening and transplanting survival rate can reach 83.3%. The propagation speed in the culture process is high, the propagation coefficient is large and is not limited by seasons and propagation materials, a large number of plants can be obtained in a short time, and the method has important significance for mass propagation production of hydrangea macrophylla.
Although the above embodiments have been described in detail with respect to the purpose of illustrating the invention, it will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the scope of the invention as defined in the appended claims.
Claims (10)
1. A quick propagation method of hydrangea macrophylla is characterized in that: the method comprises the following steps:
(1) taking the stem segment of hydrangea as an explant, and carrying out sterile disinfection treatment;
(2) cutting the hydrangea stem segments treated in the step (1) into hydrangea stem segments with at least one axillary bud, inoculating the hydrangea stem segments into a culture medium, and germinating the axillary buds;
(3) when the axillary buds in the step (2) extend to a certain length, cutting off and inoculating the axillary buds into a culture medium to proliferate the axillary buds to obtain a large number of axillary buds;
(4) inoculating the axillary buds obtained in the step (3) to a culture medium for induction culture to form rooted seedlings;
(5) transplanting the tissue culture seedling in the step (4), namely the rooted seedling, into a substrate for culturing to obtain the hydrangea aseptic seedling with strong roots and stems.
2. The method for rapid propagation of hydrangea macrophylla according to claim 1, comprising the steps of: the components of the culture medium in the step (2) are as follows: MS 1.5-2.0mg/L, 6-BA1.5-2.0mg/L, KT 0-0.5mg/L, IBA 0.3.3-0.5 mg/L, sucrose 30g/L and agar 6g/L, and pH is controlled at 5.8-6.0.
3. The method for rapid propagation of hydrangea macrophylla according to claim 2, wherein: the axillary buds are germinated by using the culture medium under the conditions that the temperature is 25 +/-1 ℃, the illumination intensity is 2500Lx and the illumination time is 12 h/d.
4. The method for rapid propagation of hydrangea macrophylla according to claim 1, comprising the steps of: the components of the culture medium in the step (3) are as follows: MS 1.5-2.0mg/L, 6-BA1.5-2.0mg/L, GA 0-0.5mg/L, IBA 0.1.1-0.3 mg/L, sucrose 30g/L and agar 6g/L, and pH is controlled at 5.8-6.0.
5. The method for rapid propagation of hydrangea macrophylla according to claim 4, wherein: the culture medium is used for carrying out the proliferation of axillary buds under the conditions of 25 +/-1 ℃, the illumination intensity of 2500Lx and the illumination time of 12 h/d.
6. The method for rapid propagation of hydrangea macrophylla according to claim 1, comprising the steps of: the components of the culture medium in the step (4) are as follows: 1/2 MS 0.1-0.3mg/L, IBA 0.1-0.3mg/L, sucrose 30g/L, agar 6g/L, pH controlled at 5.8-6.0.
7. The method for rapid propagation of hydrangea macrophylla according to claim 6, wherein: the culture medium is used for inducing the axillary buds to root under the conditions of the temperature of 23 +/-2 ℃, the illumination intensity of 1800Lx and the illumination time of 12 h/d.
8. The method for rapid propagation of hydrangea macrophylla according to claim 1, comprising the steps of: the matrix in the step (5) comprises the following components: the ratio of the turfy soil to the vermiculite to the perlite is 1:1: 1.
9. The method for rapid propagation of hydrangea macrophylla according to claim 8, wherein: the substrate is used for culturing under the conditions of the temperature of 23 +/-2 ℃, the illumination intensity of 1800Lx and the illumination time of 12 h/d.
10. The method for rapid propagation of hydrangea macrophylla according to claim 1, comprising the steps of: and (3) cutting off and inoculating after the axillary buds in the step (2) stretch to 2-3 cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010061211.1A CN111587784A (en) | 2020-01-19 | 2020-01-19 | Rapid propagation method of hydrangea macrophylla |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042540A (en) * | 2020-09-15 | 2020-12-08 | 内蒙古蒙荣园林绿化工程有限责任公司 | Rapid propagation method of hydrangea macrophylla |
| CN112273229A (en) * | 2020-10-28 | 2021-01-29 | 上海辰山植物园 | One-step seedling method for hydrangea |
-
2020
- 2020-01-19 CN CN202010061211.1A patent/CN111587784A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 王海娥: "朗姆系绣球的组织培养及植株再生体系研究", 《中国优秀硕士学位论文全文数据库（农业科技辑）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042540A (en) * | 2020-09-15 | 2020-12-08 | 内蒙古蒙荣园林绿化工程有限责任公司 | Rapid propagation method of hydrangea macrophylla |
| CN112273229A (en) * | 2020-10-28 | 2021-01-29 | 上海辰山植物园 | One-step seedling method for hydrangea |
| CN112273229B (en) * | 2020-10-28 | 2022-02-22 | 上海辰山植物园 | One-step seedling method for hydrangea |
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