CN102613076A - Vegetative propagation method for butterfly orchid - Google Patents
Vegetative propagation method for butterfly orchid Download PDFInfo
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- CN102613076A CN102613076A CN2012100810743A CN201210081074A CN102613076A CN 102613076 A CN102613076 A CN 102613076A CN 2012100810743 A CN2012100810743 A CN 2012100810743A CN 201210081074 A CN201210081074 A CN 201210081074A CN 102613076 A CN102613076 A CN 102613076A
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Abstract
The invention relates to a vegetative propagation method for cattleya, in particular to a vegetative propagation method for butterfly orchid. The vegetative propagation method for butterfly orchid, provided by the invention, comprises the following steps of: by taking pedicel and axillary bud of butterfly orchid as explant, inoculating the explant into a culture medium for culturing; and performing subculture, rooting culture and transplanting culture on the induced new buds in turn. According to the method provided by the invention, the tillering of the butterfly orchid is realized in an artificially prepared culture medium; the single-stalk reproduction feature of the butterfly orchid is broken through; an induced pedicel seedling can be obtained without hurting a butterfly orchid stock plant according to the method; and the pedicel of the same stock plant can be repeatedly used. The seedlings produced according to the method are robust, trim and consistent; the source problem of excellent butterfly orchid seedlings during production is solved; and more economic and social benefits can be created.
Description
Technical field
The present invention relates to the asexual reproduction method of the asexual reproduction method of a kind of cattleya, particularly Moth orchid.
Background technology
Moth orchid (Phalaenopsis sp.) is the orchid family butterfly Cymbidium perennial evergreen herbaceous plant that grows nonparasitically upon another plant, and belongs to tropical aerial orchid, easily cultivation; Flower likeness in form butterfly, form is graceful, rich color; Florescence is quite long, have the good reputation of " cattleya queen ", is one of most popular in recent years cattleya.Moth orchid is single stem property aerial orchid, and self does not tiller plant, seldom grows lateral bud, because the particularity of its floral organ structure can not be accomplished under the natural conditions and be pollinated, so is difficult to obtain seed; Carry out artificial pollination and obtain seed, the germination rate of seed also has only about 20%, on synthetic medium, induces its seed germination, and for new quick breeding approach has been opened up in the development of Moth orchid industrialization, but the seedling that breeds variation is many, and seedling early growth is inconsistent.The conventional vegetative propagation of Moth orchid can't be carried out basically; Therefore utilize the auxiliary Moth orchid of tissue culture means to carry out unconventional vegetative propagation (just bud is cut the bud breeding); Obtain high-quality Moth orchid seedling; Thereby the raising reproduction speed is to solve at present to produce the important means that goes up a large amount of Moth orchid seedlings that need.
Summary of the invention
To the problems referred to above, the invention provides a kind of asexual reproduction method of Moth orchid.
In order to realize above-mentioned purpose of the present invention; The present invention adopts following technical scheme; It, is inoculated into and carries out the bud inducing culture in the medium as explant with Moth orchid bennet axillalry bud, then the sprouting that induces is carried out successive transfer culture, culture of rootage and transplanting successively and cultivates.
Axillalry bud is the axillalry bud of Moth orchid bennet base portion 4～5 joints.
Concrete incubation step does,
(1) part of the wounded in the battle bud of Moth orchid bennet is cut, other part is carried out sterilization processing after cleaning; After the sterilization, upward the inserting of the bud point of bennet is seeded in the bud inducing culture, inoculum concentration is 0.1～0.2g/ml
Medium,, cultivated 30～40 days under intensity of illumination 2000～3000Lux condition 23～27 ℃ of temperature; Make it in the medium of manual work preparation, to realize tillering;
(2) bud that step (1) is induced downcuts from the bennet section, and 2～3 strains are one group, is inoculated into to carry out successive transfer culture in the inducing clumping bud medium, and inoculum concentration is 0.1～0.2g/ml
Medium, 23～27 ℃ of temperature, to cultivate under intensity of illumination 2000～3000Lux condition, subculture cycle is 40～50 days;
(3) bud with 9 generations of subculture is cut into independently individual plant, is seeded in and carries out culture of rootage in the root media, and inoculum concentration is 0.1～0.2g/ml
Medium, 23～27 ℃ of temperature, cultivate under intensity of illumination 2000～3000Lux condition, can transplant by bottle outlet after 2～3 months.
Moth orchid bennet described in the step (1) is during existing petal is not bloomed, bloomed or the bennet of the faded Moth orchid of flower.
Sterilization processing step described in the step (1) is that on superclean bench, the bennet that flushing is good cuts into the long segment of 5cm; Every section has an axillalry bud, and the bract on the axillalry bud is peelled off, and at first in the alcoholic solution of 75wt%, soaks; Blot alcohol with filter paper; Put into the mercuric chloride solution sterilization 8～15 minutes of 0.1wt% concentration then, use aseptic water washing at last 3～4 times, blot water with filter paper and be placed in the culture dish subsequent use.
Consisting of of bud inducing culture described in the step (1), 1/3MS or spend precious No. 1 2.5～3.5gl
-1+ BA 1～3mgl
-1+ NAA 0.1～0.3mgl
-1+ sucrose 20～30gl
-1+ banana 30～50gl
-1
Inducing clumping bud medium described in the step (2) consist of 1/2MS+BA0.1～0.5mgl
-1+ sucrose 20～30gl
-1
Consisting of of root media described in the step (3), 1/2MS or spend precious No. 1 2.5～3.5gl
-1+ NAA 0～1mgl
-1+ sucrose 20～30gl
-1+ active carbon 1～2gl
-1+ banana 100～200gl
-1
It is that 2～3 roots, the number of blade are arranged is 2～3 Moth orchid tissue cultivating seedling that bottle outlet described in the step (3) is transplanted what choose.
Beneficial effect of the present invention:
The present invention has announced a kind of asexual reproduction method of Moth orchid; Make Moth orchid in the medium of manual work preparation, realize tillering; The reproduction characteristics of Moth orchid list stem property have been broken; The method need not injure the Moth orchid maternal plant can obtain the bennet seedling that is used for inducing, and can use the bennet of same maternal plant repeatedly.The seedling that the present invention produces is healthy and strong, neat and consistent, can solve to produce the source problem that comes of going up high-quality Moth orchid seedling, and can create bigger economic and social benefit.
Embodiment:
Embodiment 1
Choose and just take out the bennet of the ripe maternal plant of Moth orchid " thousand favour roses " that colored arrow has petal, clean, be placed on then under the flowing water and washed 30～40 minutes with washing powder water as explant; On superclean bench, will wash good bennet and cut into the long segment of 5cm; Every section has an axillalry bud, and the bract on the axillalry bud is peelled off, and at first in the alcoholic solution of 75wt%, soaks; Blot alcohol with filter paper; Put into the mercuric chloride solution sterilization 8 minutes of 0.1wt% concentration then, use aseptic water washing at last 3 times, blot water with filter paper and be placed in the culture dish.
0.5cm is respectively downcut in the bennet both sides, and upward the inserting of bud point is seeded in the bud inducing culture then, and inoculum concentration is 0.1g/ml
Medium, 23 ℃ of temperature, cultivated under the condition of intensity of illumination 3000Lux 30 days, the bud inducing culture consist of 1/3MS+BA 1mgl
-1+ NAA0.1mgl
-1+ sucrose 20gl
-1+ banana 50gl
-1Each bud point can be induced 1 young plant, and only a few can induce 2 young plants.
The bud that induces is downcut from the bennet section, need not the cutting of 2 buds, connecting together is inoculated in the inducing clumping bud medium, and inoculum concentration is 0.1g/ml
Medium,, 27 ℃ of temperature, carry out successive transfer culture under the condition of intensity of illumination 3000Lux,, subculture cycle is 40 days, the inducing clumping bud medium consist of 1/2MS+BA 0.5mgl
-1+ sucrose 30gl
-1
The bud in 9 generations of subculture is cut into independently individual plant, is seeded in the root media, inoculum concentration is 0.1g/ml
Medium,, 27 ℃ of temperature, carry out culture of rootage under the intensity of illumination 2000Lux condition, root media consist of 1/2MS+NAA 1mgl
-1+ sucrose 30gl
-1+ active carbon 1gl
-1+ banana 100gl
-1What choose after 2 months is that 2～3 roots, the number of blade are arranged is that 2～3 Moth orchid tissue cultivating seedling carries out bottle outlet and transplants.
Embodiment 2
The bennet of choosing the ripe maternal plant of Moth orchid " huge precious red rose " that is blooming cleans with washing powder water as explant, is placed on then under the flowing water and washes 30～40 minutes; On superclean bench, will wash good bennet and cut into the long segment of 5cm; Every section has an axillalry bud, and the bract on the axillalry bud is peelled off, and at first in the alcoholic solution of 75wt%, soaks; Blot alcohol with filter paper; Put into the mercuric chloride solution sterilization 10 minutes of 0.1wt% concentration then, use aseptic water washing at last 3～4 times, blot water with filter paper and be placed in the culture dish.
0.5cm is respectively downcut in the bennet both sides, and upward the inserting of bud point is seeded in the bud inducing culture then, and inoculum concentration is 0.2g/ml
Medium,, 27 ℃ of temperature, to cultivate 40 days under the condition of intensity of illumination 3000Lux, precious No. 1 3.5gl is spent in consisting of of bud inducing culture
-1+ BA 3mgl
-1+ NAA 0.2mgl
-1+ sucrose 30gl
-1+ banana 30gl
-1Each bud point can be induced 1 young plant, and only a few can induce 2 young plants.
The bud that induces is downcut from the bennet section, need not the cutting of 2 buds, connecting together is inoculated in the inducing clumping bud medium, and inoculum concentration is 0.1g/ml
Medium,, be 25 ℃ in temperature, carry out successive transfer culture under the condition of intensity of illumination 3000Lux, subculture cycle is 40 days, the inducing clumping bud medium consist of 1/2MS+BA 0.1mgl
-1+ sucrose 20gl
-1
The bud in 9 generations of subculture is cut into independently individual plant, is seeded in the root media, inoculum concentration is 0.2g/ml
Medium,, be 25 ℃ in temperature, carry out culture of rootage under the condition of intensity of illumination 2000Lux, root media consist of 1/2MS+NAA 0.5mgl
-1+ sucrose 20gl
-1+ active carbon 1gl
-1+ banana 200gl
-1What choose after 3 months is that 2～3 roots, the number of blade are arranged is that 2～3 Moth orchid tissue cultivating seedling carries out bottle outlet and transplants.
Embodiment 3
The bennet of choosing the petal ripe maternal plant of wide-open Moth orchid " red crown prince " cleans with washing powder water as explant, is placed on then under the flowing water to wash 30～40 minutes; On superclean bench, will wash good bennet and cut into the long segment of 5cm; Every section has an axillalry bud, and the bract on the axillalry bud is peelled off, and at first in the alcoholic solution of 75wt%, soaks; Blot alcohol with filter paper; Put into the mercuric chloride solution sterilization 12 minutes of 0.1wt% concentration then, use aseptic water washing at last 3～4 times, blot water with filter paper and be placed in the culture dish.
0.5cm is respectively downcut in the bennet both sides, and upward the inserting of bud point is seeded in the bud inducing culture then, and inoculum concentration is 0.1g/ml
Medium,, 23 ℃ of temperature, to cultivate 30 days under the condition of intensity of illumination 3000Lux, precious No. 1 2.5gl is spent in consisting of of bud inducing culture
-1+ BA 2mgl
-1+ NAA 0.3mgl
-1+ sucrose 20gl
-1+ banana 30gl
-1Each bud point can be induced 1 young plant, and only a few can induce 2 young plants.
The bud that induces is downcut from the bennet section, and need not cutting of 2 buds connects together to be inoculated into and carries out successive transfer culture in the inducing clumping bud medium, and inoculum concentration is 0.2g/ml
Medium,, be 25 ℃ in temperature, carry out successive transfer culture under the condition of intensity of illumination 3000Lux, subculture cycle is 50 days, the inducing clumping bud medium consist of 1/2MS+BA 0.3mgl
-1+ sucrose 30gl
-1
The bud in 9 generations of subculture is cut into independently individual plant, is seeded in and carries out culture of rootage in the root media, inoculum concentration is 0.1g/ml
Medium,, be 27 ℃ in temperature, carry out successive transfer culture under the condition of intensity of illumination 3000Lux, precious No. 1 3.5gl is spent in consisting of of root media
-1+ NAA0.2mgl
-1+ sucrose 30gl
-1+ active carbon 1gl
-1+ banana 200gl
-1What choose after 3 months is that 2～3 roots, the number of blade are arranged is that 2～3 Moth orchid tissue cultivating seedling carries out bottle outlet and transplants.
Embodiment 4
The bennet of choosing the petal ripe maternal plant of faded Moth orchid " red dragon " cleans with washing powder water as explant, is placed on then under the flowing water to wash 30～40 minutes; On superclean bench, will wash good bennet and cut into the long segment of 5cm; Every section has an axillalry bud, and the bract on the axillalry bud is peelled off, and at first in the alcoholic solution of 75wt%, soaks; Blot alcohol with filter paper; Put into the mercuric chloride solution sterilization 15 minutes of 0.1wt% concentration then, use aseptic water washing at last 3～4 times, blot water with filter paper and be placed in the culture dish.
0.5cm is respectively downcut in the bennet both sides, and upward the inserting of bud point is seeded in the bud inducing culture then.Inoculum concentration is 0.1g/ml
Medium,, 27 ℃ of temperature, cultivated under the condition of intensity of illumination 3000Lux 40 days, the bud inducing culture consist of 1/3MS+BA 1mgl
-1+ NAA 0.3mgl
-1+ sucrose 20gl
-1+ banana 50gl
-1Each bud point can be induced 1 young plant, and only a few can induce 2 young plants.
The bud that induces is downcut from the bennet section, and need not cutting of 2 buds connects together to be inoculated into and carries out successive transfer culture in the inducing clumping bud medium.Inoculum concentration is 0.1g/ml
Medium,, 27 ℃ of temperature, carry out successive transfer culture under the condition of intensity of illumination 3000Lux, subculture cycle is 50 days, the inducing clumping bud medium consist of 1/2MS+BA 0.2mgl
-1+ sucrose 30gl
-1
The bud in 9 generations of subculture is cut into independently individual plant, is seeded in and carries out culture of rootage in the root media, inoculum concentration is 0.1g/ml
Medium,, 27 ℃ of temperature, carrying out culture of rootage under intensity of illumination 2000 conditions, precious No. 1 2.5gl is spent in consisting of of root media
-1+ sucrose 30gl
-1+ active carbon 2gl
-1+ banana 100gl
-1What choose after 3 months is that 2～3 roots, the number of blade are arranged is that 2～3 Moth orchid tissue cultivating seedling carries out bottle outlet and transplants.
" thousand favour roses " among the embodiment, " huge precious red rose ", " red crown prince ", " red dragon " are available from middle ancient cooking vessel biotechnology (Shanghai) Co., Ltd..
Claims (8)
1. the asexual reproduction method of a Moth orchid is characterized in that,, is inoculated into and carries out the bud inducing culture in the medium as explant with Moth orchid bennet axillalry bud, then the sprouting that induces is carried out successive transfer culture, culture of rootage and transplanting successively and cultivates.
2. the asexual reproduction method of Moth orchid according to claim 1 is characterized in that,
Concrete incubation step does,
(1) part of the wounded in the battle bud of Moth orchid bennet is cut, other part is carried out sterilization processing after cleaning; After the sterilization, upward the inserting of the bud point of bennet is seeded in the bud inducing culture, inoculum concentration is 0.1～0.2g/ml
Medium,, cultivated under the condition of intensity of illumination 2000～3000Lux 30～40 days 23～27 ℃ of temperature;
(2) bud that step (1) is induced downcuts from the bennet section, and 2～3 strains are one group, is inoculated into to carry out successive transfer culture in the inducing clumping bud medium, and inoculum concentration is 0.1～0.2g/ml
Medium, 23～27 ℃ of temperature, to cultivate under the condition of intensity of illumination 2000～3000Lux, subculture cycle is 40～50 days;
(3) bud with 9 generations of subculture is cut into independently individual plant, is seeded in and carries out culture of rootage in the root media, and inoculum concentration is 0.1～0.2g/ml
Medium, 23～27 ℃ of temperature, cultivate under the condition of intensity of illumination 2000～3000Lux, can transplant by bottle outlet after 2～3 months.
3. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, the Moth orchid bennet described in the step (1) is during existing petal is not bloomed, bloomed or the bennet of the faded Moth orchid of flower.
4. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, the sterilization processing step described in the step (1) does; On superclean bench, the bennet that flushing is good cuts into the long segment of 5cm, and every section has an axillalry bud; Bract on the axillalry bud is peelled off; At first in the alcoholic solution of 75wt%, soak, blot alcohol, put into the mercuric chloride solution sterilization 8～15 minutes of 0.1wt% concentration then with filter paper; Use aseptic water washing at last 3～4 times, blot water with filter paper and be placed in the culture dish subsequent use.
5. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, the consisting of of the bud inducing culture described in the step (1), 1/3MS or spend precious No. 1 2.5～3.5gl
-1+ BA 1～3mgl
-1+ NAA 0.1～0.3mgl
-1+ sucrose 20～30gl
-1+ banana 30～50gl
-1
6. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, the inducing clumping bud medium described in the step (2) consist of 1/2MS+BA 0.1～0.5mgl
-1+ sucrose 20～30gl
-1
7. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, the consisting of of the root media described in the step (3), 1/2MS or spend precious No. 1 2.5～3.5gl
-1+ NAA 0～1mgl
-1+ sucrose 20～30gl
-1+ active carbon 1～2gl
-1+ banana 100～200gl
-1
8. the asexual reproduction method of Moth orchid according to claim 2 is characterized in that, it is that 2～3 roots, the number of blade are arranged is 2～3 Moth orchid tissue cultivating seedling that bottle outlet described in the step (3) is transplanted what choose.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
| CN103782911A (en) * | 2014-02-07 | 2014-05-14 | 福建省亚热带植物研究所 | Regulating and controlling method for synchronization of somatic embryo of butterfly orchid |
| CN103798136A (en) * | 2013-12-11 | 2014-05-21 | 柳州赛特生物科技研发中心 | Special phalaenopsis tissue culture medium |
| CN104067943A (en) * | 2014-07-18 | 2014-10-01 | 内蒙古农业大学 | Phalaenopsis sterile root propagation method |
| CN104082148A (en) * | 2014-07-18 | 2014-10-08 | 内蒙古农业大学 | Method for performing regeneration propagation on sterile stalks of butterfly orchids |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN105941161A (en) * | 2016-06-25 | 2016-09-21 | 合肥隆扬农业科技有限公司 | Tissue culture method of phalaenopsis |
| CN106489461A (en) * | 2015-09-07 | 2017-03-15 | 湖南瑞利农业发展有限公司 | A kind of breeding method of iris bennet Seedling |
| CN106942062A (en) * | 2017-04-26 | 2017-07-14 | 江苏农林职业技术学院 | A kind of iris sterile culture strain butt tissue culture method |
| CN107278901A (en) * | 2017-07-31 | 2017-10-24 | 王生旭 | A kind of sterilization method of iris bennet bud |
| CN107581068A (en) * | 2017-10-13 | 2018-01-16 | 中国科学院华南植物园 | A kind of method for removing single-shaft orchid endophyte and the application in vanilla tissue-culturing quick-propagation |
| CN107996344A (en) * | 2017-12-10 | 2018-05-08 | 广西长耀隆农业科技有限公司 | A kind of cultural method of potted plant iris |
| CN115251057A (en) * | 2022-08-26 | 2022-11-01 | 郑州师范学院 | Method for inducing germination of phalaenopsis seedlings by utilizing phytohormone composition |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0800762A1 (en) * | 1996-04-03 | 1997-10-15 | Sapporo Breweries Ltd. | Method for producing phalaenopsis clone plants through root tip culture |
| CN1586165A (en) * | 2004-07-30 | 2005-03-02 | 中国科学院华南植物园 | Quick breeding method for butterfly orchid high quality sprout |
| CN1994068A (en) * | 2006-12-27 | 2007-07-11 | 李�杰 | Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel |
| CN101857496A (en) * | 2010-06-02 | 2010-10-13 | 福建新世景园艺有限公司 | Moth orchid culture medium |
| CN102283110A (en) * | 2010-09-29 | 2011-12-21 | 深圳市农科植物克隆种苗有限公司 | Method for rapidly propagating moth orchids through inducing cluster buds by leaves |
-
2012
- 2012-03-26 CN CN2012100810743A patent/CN102613076A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0800762A1 (en) * | 1996-04-03 | 1997-10-15 | Sapporo Breweries Ltd. | Method for producing phalaenopsis clone plants through root tip culture |
| CN1586165A (en) * | 2004-07-30 | 2005-03-02 | 中国科学院华南植物园 | Quick breeding method for butterfly orchid high quality sprout |
| CN1994068A (en) * | 2006-12-27 | 2007-07-11 | 李�杰 | Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel |
| CN101857496A (en) * | 2010-06-02 | 2010-10-13 | 福建新世景园艺有限公司 | Moth orchid culture medium |
| CN102283110A (en) * | 2010-09-29 | 2011-12-21 | 深圳市农科植物克隆种苗有限公司 | Method for rapidly propagating moth orchids through inducing cluster buds by leaves |
Non-Patent Citations (2)
| Title |
|---|
| 《宿州教育学院学报》 20111031 柳丽 蝴蝶兰的组织培养研究 第91页右栏2段，第92页左栏第2、5段，第93段左栏第1段 7 第14卷, 第5期 * |
| 柳丽等: "蝴蝶兰的组织培养研究", 《宿州教育学院学报》 * |
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| CN103798136A (en) * | 2013-12-11 | 2014-05-21 | 柳州赛特生物科技研发中心 | Special phalaenopsis tissue culture medium |
| CN103798136B (en) * | 2013-12-11 | 2016-04-13 | 柳州赛特生物科技研发中心 | A kind of butterfly orchid private organizations culture medium |
| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
| CN103734008B (en) * | 2013-12-26 | 2016-04-13 | 广西森仙源生物科技有限公司 | A kind of butterfly orchid tissue cultivating Fast-propagation liquid nutrient medium and corresponding cultural method |
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| CN103782911A (en) * | 2014-02-07 | 2014-05-14 | 福建省亚热带植物研究所 | Regulating and controlling method for synchronization of somatic embryo of butterfly orchid |
| CN104082148A (en) * | 2014-07-18 | 2014-10-08 | 内蒙古农业大学 | Method for performing regeneration propagation on sterile stalks of butterfly orchids |
| CN104067943A (en) * | 2014-07-18 | 2014-10-01 | 内蒙古农业大学 | Phalaenopsis sterile root propagation method |
| CN104082148B (en) * | 2014-07-18 | 2016-08-17 | 内蒙古农业大学 | The method of iris aseptic bennet regeneration expanding propagation |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN106489461A (en) * | 2015-09-07 | 2017-03-15 | 湖南瑞利农业发展有限公司 | A kind of breeding method of iris bennet Seedling |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN105941161A (en) * | 2016-06-25 | 2016-09-21 | 合肥隆扬农业科技有限公司 | Tissue culture method of phalaenopsis |
| CN106942062A (en) * | 2017-04-26 | 2017-07-14 | 江苏农林职业技术学院 | A kind of iris sterile culture strain butt tissue culture method |
| CN107278901A (en) * | 2017-07-31 | 2017-10-24 | 王生旭 | A kind of sterilization method of iris bennet bud |
| CN107581068A (en) * | 2017-10-13 | 2018-01-16 | 中国科学院华南植物园 | A kind of method for removing single-shaft orchid endophyte and the application in vanilla tissue-culturing quick-propagation |
| CN107996344A (en) * | 2017-12-10 | 2018-05-08 | 广西长耀隆农业科技有限公司 | A kind of cultural method of potted plant iris |
| CN115251057A (en) * | 2022-08-26 | 2022-11-01 | 郑州师范学院 | Method for inducing germination of phalaenopsis seedlings by utilizing phytohormone composition |
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