CN1586165A - Quick breeding method for butterfly orchid high quality sprout - Google Patents
Quick breeding method for butterfly orchid high quality sprout Download PDFInfo
- Publication number
- CN1586165A CN1586165A CN 200410050906 CN200410050906A CN1586165A CN 1586165 A CN1586165 A CN 1586165A CN 200410050906 CN200410050906 CN 200410050906 CN 200410050906 A CN200410050906 A CN 200410050906A CN 1586165 A CN1586165 A CN 1586165A
- Authority
- CN
- China
- Prior art keywords
- seedling
- bud
- bennet
- moth orchid
- high quality
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to the quick breeding method of high quality seedling of Phalaenopsis, known as 'queen orchid' and the culture medium therewith. The present invention uses the flower stem section of Phalaenopsis as explant and induces flower stem seedling with unique culture medium and performs proliferation with fascicular bud stem tip or stem section to produce high quality seedling of Phalaenopsis. The method is simple, practical, feasible and high in application value.
Description
Technical field
The present invention relates to the high quality seedling method for quickly breeding and the employed medium of Moth orchid.
Background technology
Moth orchid (Phalaenopsis) claim phalaenopsis again, is to cultivate one of extensive, the most universal kind in the orchid, is described as " orchid queen ".This platymiscium whole world has more than 40 initial species approximately; there are 6 initial specieses in China; but since people to wild resource over-exploitation; wherein most of wild initial species has been difficult to find now, and the Moth orchid initial species is also classified as one of the world's ten big protective plant species in imminent danger by world's conservation of wild plant tissue.At present, Moth orchid in different poses and with different expressions on the market, large flower and brilliant color is viewed and admired to plant and mostly is crossbreed, and can belong to hybridization formation intergeneric cross kind with other, as hybridizing formation intergeneric cross kind Doritaenopsis (being abbreviated as Dtps.) with Duo Lilan (Doritis).Because the crossbreed florescence is long, pattern is gorgeous, flower appearance gracefulness, and growth potential is strong, is subjected to liking of various countries people deeply, is a kind of very famous and precious indoor flower, and it not only can be made cut-flower but also can potted plantly view and admire, and is all the fashion in China in recent years.The large-scale commercial production of Moth orchid is quite successful on China Taiwan, Japan and other places, all has every year hundreds and thousands of new varieties to put goods on the market and exports.The industry of China mainland Moth orchid is started late, but unit or the company that has many production Moth orchides in all parts of the country now, yet, the Moth orchid seedling of China many from ground import such as Taiwan or Japan or these companies in China mainland production, it is less that China mainland has the butterfly orchid variety of self-produced intellectual property, and the Moth orchid seedling quality of production is relatively poor.
Moth orchid belongs to single stem aerial orchid, and lateral bud is seldom grown, and the division propagation coefficient is extremely low.Its main propagation method adopts aseptic seeding and passes through evoking adventive bud breeding again such as protocorm, callus.But when high-quality Moth orchid crossbreed utilized the aseptic seeding breeding, the offspring can separate, and can not keep the homogeneity of the merit and the seedling of parent.And adopt protocorm, callus etc. again during evoking adventive bud breeding seedling, can increase the coefficient of variation of seedling.Patent of the present invention is an explant with the bennet sections of Moth orchid, utilize unique medium to induce the bennet seedling, be propagation with the stem apex of bennet seedling or the stem section bud of growing thickly again, produce the Moth orchid seedling of high-quality, for the needs that satisfy market provide a valid approach.
At present, the aseptic seeding sowing and the tissue culture of Moth orchid have been carried out more research both at home and abroad, but the report that carries out quick breeding of Moth orchid high quality seedling and large-scale production by the bud approach of growing thickly has not been arranged.
Summary of the invention
Aseptic seeding and tissue culture are often adopted in the breeding of Moth orchid, but aseptic seeding and can not preserve parent's merit when breeding with the protocorm approach.The objective of the invention is by the bennet sections with Moth orchid is explant, utilize unique medium to induce the bennet seedling, be propagation with the stem apex of bennet seedling or the stem section bud of growing thickly again, produce the Moth orchid seedling of high-quality, carry out the cultivation of knitting of Moth orchid improved seeds with the bud approach of growing thickly, obtain the stable test-tube plantlet of a large amount of proterties, for the needs that satisfy Moth orchid high quality seedling market provide a valid approach, the present invention also provides grow thickly bud tissue culture propagation and the employed medium of multiple Moth orchid hybrid, reaches the selection to explant.
Moth orchid high quality seedling method for quickly breeding of the present invention comprises the steps:
1. material: the used material of the present invention has that red over the sky (Dtps.Queen Beer " Red Sky ", super red (Phalaenopsis Super-Red) etc., material is all taken from the South China Botanical Garden.
2. the sterilization of explant: the bennet of getting the Moth orchid bud, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, again with 0.1% mercuric chloride solution sterilization 15 minutes, behind the aseptic water washing 5 times, blot with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture.
3. inducing of bennet seedling: the inducing culture of bennet seedling is VW+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre, left and right sides sleeping bud started growth in 15 days, develop into bud when cultivation temperature (20 ± 2) ℃, multiform becomes the bennet seedling of band leaf when cultivation temperature (28 ± 2) ℃.Medium contains sucrose 15 grams per liters, pH5.5-5.8, agar 0.7%.Illuminance 1500～2000lx, illumination 12 hours/day.Add sodium citrate energy inhibition of browning, improve the growth rate of bennet seedling.
4. adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling, cut off partial blade and transfer on adventitious buds proliferation medium MS+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre+15% Sucus Cocois+sucrose 30 grams per liter medium the formation of can induced bundle sprouting, Sucus Cocois adds coconut milk and can obviously improve sprouting and growth rate of the bud of growing thickly by the juice in the coconut of fresh listing after filtration.The indefinite bud clump that induces is divided into one clump of single bud or several bud, is seeded on the same medium and breeds.If have protocorm to form in the breeding, need to reduce the concentration of 6-benzyl purine.
5. culture of rootage: adventitious buds proliferation is after many generations, the bud of growing thickly can be cut into single bud, be seeded in to contain on the root media of spending precious (Hyponex 1) 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+horse bell alpine yarrow 50-100 grams per liter+activated carbon 2 grams per liters and carry out culture of rootage.Can form plant height 3-4 centimetre whole plant about 40 days.Adding active carbon, to help plant strain growth sturdy and can prevent brown stain.
6. test-tube seedling transplanting: spring and autumn the favorable season for transplanting.During test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity, and the survival rate of transplanting all can reach 98%-100%.
The Moth orchid seedling that the present invention produces has the genetic stability height, and it is fast to emerge, and seedling is strong, advantages such as seedling quality better, resistance, well-grown.The present invention utilizes the bennet of Moth orchid to be explant, and the unique medium of employing carries out adventitious buds proliferation and obtains the method for high quality seedling, and technology is material benefit simply, and is practical, the using value height.Moth orchid is to cultivate one of extensive, the most universal kind in the orchid, is described as " orchid queen ".Its crossbreed has features such as the florescence is long, and pattern is gorgeous, spends the appearance gracefulness, and growth potential is strong, is subjected to liking of various countries people deeply, is a kind of very famous and precious indoor flower, and it not only can be made cut-flower but also can potted plantly view and admire, and is all the fashion in China in recent years, and the plantation amount is big.It is less that China mainland has the butterfly orchid variety of self-produced intellectual property, and the Moth orchid seedling quality of production is relatively poor.If produce the Moth orchid seedling of high-quality, market prospects are wide.
Embodiment
Embodiment 1
1. draw materials: butterfly orchid variety sunset (Phalaenopsis Taida Salu " sunset ")
2. the sterilization of explant: the bennet of getting safflower bud all over the sky, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, used 0.1% (percentage by weight) mercuric chloride solution sterilization again 15 minutes, and behind the aseptic water washing 5 times, blotted with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture.
3. inducing of bennet seedling: the inducing culture of bennet seedling is VW+6-benzyl purine 10 mg/litre+methyl 0.5 mg/litre+sodium citrate 150 mg/litre, and sleeping bud started growth in 15 days, and multiform becomes the bennet seedling of band leaf when cultivation temperature (28 ± 2) ℃.
4. adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling cuts off partial blade and transfers on adventitious buds proliferation medium MS+6-benzyl purine 10 mg/litre+methyl 0.5 mg/litre+150 sodium citrates+15% Sucus Cocois+sucrose 30 grams per liter medium the formation of can induced bundle sprouting.The indefinite bud clump that induces is divided into one clump of single bud or several bud, is seeded on the same medium and breeds.
5. culture of rootage: adventitious buds proliferation is after many generations, the bud of growing thickly can be cut into single bud, be seeded in to contain on the root media of spending precious (Hyponex 1) 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+horse bell alpine yarrow 100 grams per liters+activated carbon 2 grams per liters and carry out culture of rootage.Can form plant height 3-4 centimetre whole plant about 40 days.
6. test-tube seedling transplanting: spring and autumn the favorable season for transplanting.During test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity, and the survival rate of transplanting all can reach 98%-100%.
Taking from the main high butterfly orchid variety of breeding super red (Dtps Super-Red) of ornamental value is explant.The basic operation method is with embodiment 1 unanimity.But medium component is slightly different.The inducing culture of its bennet seedling is VW+6-benzyl purine 15 mg/litre+methyl 1.0 mg/litre+sodium citrate 100 mg/litre.The adventitious buds proliferation medium is MS+6 benzyl purine 15 mg/litre+methyl 0 mg/litre+sodium citrate 100 mg/litre+15% Sucus Cocois+sucrose 30 grams per liters.The survival rate of test-tube seedling transplanting can reach 100%.
Claims (7)
1, a kind of Moth orchid high quality seedling method for quickly breeding is characterized in that the bennet sections with Moth orchid is an explant, utilizes unique medium to induce the bennet seedling, is propagation with the stem apex of bennet seedling or the stem section bud of growing thickly again, and concrete steps comprise as follows:
1), the sterilization of explant: the bennet of getting the Moth orchid bud, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, again with percentage by weight 0.1% mercuric chloride solution sterilization 15 minutes, behind the aseptic water washing 5 times, blot with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture;
2), the inducing of bennet seedling: the bennet seedling is on inducing culture, and left and right sides sleeping bud started growth in 15 days, develops into bud when 20 ± 2 ℃ of cultivation temperature, multiform becomes the bennet seedling of band leaf when 28 ± 2 ℃ of cultivation temperature, illuminance 1500～2000lx, illumination 12 hours/day adds sodium citrate;
3), adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling cuts off partial blade and transfers on the adventitious buds proliferation medium, the formation of can induced bundle sprouting, the indefinite bud clump that induces is divided into one clump of single bud or several bud, is seeded on the same medium and breeds;
4), culture of rootage: adventitious buds proliferation can cut into single bud with the bud of growing thickly after many generations, is seeded in and carries out culture of rootage on the root media, can form plant height 3-4 centimetre whole plant in 40 days;
5), test-tube seedling transplanting: during test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity.
2, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, it is characterized in that 2) described in the inducing culture of bennet seedling be VW+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre, medium contains sucrose 15 grams per liters, pH5.5-5.8, agar 0.7%.
3, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, it is characterized in that 3) described in the adventitious buds proliferation medium be MS+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre+15% Sucus Cocois+sucrose 30 grams per liters.
4,, it is characterized in that described Sucus Cocois by the juice in the fresh coconut after filtration according to the Moth orchid high quality seedling method for quickly breeding described in the claim 3.
5, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, it is characterized in that 4) described in root media spend precious 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+horse bell alpine yarrow 50-100 grams per liter+activated carbon 2 grams per liters for containing.
6, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, it is characterized in that 5) described in test-tube plantlet be implanted in spring and autumn favorable season for transplanting.
7, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, the kind that it is characterized in that described Moth orchid is for red, super red over the sky.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410050906 CN1258321C (en) | 2004-07-30 | 2004-07-30 | Quick breeding method for butterfly orchid high quality sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410050906 CN1258321C (en) | 2004-07-30 | 2004-07-30 | Quick breeding method for butterfly orchid high quality sprout |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1586165A true CN1586165A (en) | 2005-03-02 |
| CN1258321C CN1258321C (en) | 2006-06-07 |
Family
ID=34602308
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410050906 Expired - Fee Related CN1258321C (en) | 2004-07-30 | 2004-07-30 | Quick breeding method for butterfly orchid high quality sprout |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1258321C (en) |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112175B (en) * | 2007-08-23 | 2010-05-26 | 浙江省农业科学院 | Tissue cultivation quick breeding method of arachnis labrosa |
| CN101584298B (en) * | 2009-06-02 | 2011-09-14 | 中国科学院华南植物园 | Test-tube breeding method for Nothodoritis germchit |
| CN102273408A (en) * | 2011-06-23 | 2011-12-14 | 中国科学院华南植物园 | Method for quickly breeding high-quality seedling of pschopsis |
| CN101874470B (en) * | 2009-11-12 | 2012-07-04 | 大连大学 | Method for improving reproduction coefficient of Phalaenopsis hybrid |
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN102893851A (en) * | 2012-06-19 | 2013-01-30 | 漳州钜宝生物科技有限公司 | Butterfly orchid breeding method |
| CN103172433A (en) * | 2011-12-23 | 2013-06-26 | 漳州钜宝生物科技有限公司 | Preparation method of proliferation medium for raising phalaenopsis amabilis seedlings |
| CN103202233A (en) * | 2013-04-28 | 2013-07-17 | 安徽农业大学 | Butterfly orchid anti-browning tissue culture method and anti-browning culture medium |
| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
| CN103755461A (en) * | 2013-12-23 | 2014-04-30 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for culturing orchid tissue and culture method thereof |
| CN103798136A (en) * | 2013-12-11 | 2014-05-21 | 柳州赛特生物科技研发中心 | Special phalaenopsis tissue culture medium |
| CN104067943A (en) * | 2014-07-18 | 2014-10-01 | 内蒙古农业大学 | Phalaenopsis sterile root propagation method |
| CN104082148A (en) * | 2014-07-18 | 2014-10-08 | 内蒙古农业大学 | Method for performing regeneration propagation on sterile stalks of butterfly orchids |
| CN104255494A (en) * | 2014-09-19 | 2015-01-07 | 郎溪庆林生态特色农业观光园有限公司 | Culture medium for butterfly orchid tissue culture |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN104686324A (en) * | 2013-04-28 | 2015-06-10 | 安徽农业大学 | Phalaenopsis amabilis anti-browning tissue culture method taking lemon extract as anti-browning culture medium |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN106922432A (en) * | 2017-01-23 | 2017-07-07 | 柳州市方艺种养场 | A kind of orchid cultivation method |
| CN106922512A (en) * | 2017-03-23 | 2017-07-07 | 苏州农业职业技术学院 | A kind of orchid cultural method |
| CN108077010A (en) * | 2017-12-15 | 2018-05-29 | 佛山科学技术学院 | A kind of hormone treatment method for improving early blossoming chalk Flowering quality |
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
| CN109349105A (en) * | 2018-10-10 | 2019-02-19 | 广西生态工程职业技术学院 | A kind of iris tissue-cultured seedling mating system |
| CN109392711A (en) * | 2018-11-20 | 2019-03-01 | 广东省农业科学院作物研究所 | A kind of fast breeding method of glycan seedling |
| CN110036910A (en) * | 2019-04-15 | 2019-07-23 | 中国医学科学院药用植物研究所海南分所 | A kind of blood aspidistra quick breeding by group culture method |
| CN110463556A (en) * | 2019-09-19 | 2019-11-19 | 漳州新镇宇生物科技有限公司 | A kind of iris bottle seedling refinery method |
| CN110896856A (en) * | 2019-11-07 | 2020-03-24 | 郑州师范学院 | Method for inducing complete inflorescence in vitro by axillary bud of inflorescence shaft of phalaenopsis |
-
2004
- 2004-07-30 CN CN 200410050906 patent/CN1258321C/en not_active Expired - Fee Related
Cited By (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101112175B (en) * | 2007-08-23 | 2010-05-26 | 浙江省农业科学院 | Tissue cultivation quick breeding method of arachnis labrosa |
| CN101584298B (en) * | 2009-06-02 | 2011-09-14 | 中国科学院华南植物园 | Test-tube breeding method for Nothodoritis germchit |
| CN101874470B (en) * | 2009-11-12 | 2012-07-04 | 大连大学 | Method for improving reproduction coefficient of Phalaenopsis hybrid |
| CN102273408A (en) * | 2011-06-23 | 2011-12-14 | 中国科学院华南植物园 | Method for quickly breeding high-quality seedling of pschopsis |
| CN102273408B (en) * | 2011-06-23 | 2016-06-08 | 中国科学院华南植物园 | Butterfly high quality germchit rapid breeding method of OncidiumLuridum |
| CN103172433A (en) * | 2011-12-23 | 2013-06-26 | 漳州钜宝生物科技有限公司 | Preparation method of proliferation medium for raising phalaenopsis amabilis seedlings |
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN102893851A (en) * | 2012-06-19 | 2013-01-30 | 漳州钜宝生物科技有限公司 | Butterfly orchid breeding method |
| CN104686325A (en) * | 2013-04-28 | 2015-06-10 | 安徽农业大学 | Phalaenopsis amabilis anti-browning tissue culture method taking rosmarinus officinalis extract as anti-browning culture medium |
| CN104686324A (en) * | 2013-04-28 | 2015-06-10 | 安徽农业大学 | Phalaenopsis amabilis anti-browning tissue culture method taking lemon extract as anti-browning culture medium |
| CN103749297A (en) * | 2013-04-28 | 2014-04-30 | 安徽农业大学 | Browning-prevention Phalaenopsis amabilis tissue culture method and browning-prevention culture medium |
| CN104686325B (en) * | 2013-04-28 | 2016-06-01 | 安徽农业大学 | A kind of butterfly orchid anti-browning tissue culture method taking Rosmarinus officinalis extracting solution as anti-browning substratum |
| CN103749297B (en) * | 2013-04-28 | 2015-08-05 | 安徽农业大学 | A kind of Moth orchid anti-browning tissue culture method taking tomato extract solution as anti-browning group and train medium |
| CN103202233A (en) * | 2013-04-28 | 2013-07-17 | 安徽农业大学 | Butterfly orchid anti-browning tissue culture method and anti-browning culture medium |
| CN103798136A (en) * | 2013-12-11 | 2014-05-21 | 柳州赛特生物科技研发中心 | Special phalaenopsis tissue culture medium |
| CN103798136B (en) * | 2013-12-11 | 2016-04-13 | 柳州赛特生物科技研发中心 | A kind of butterfly orchid private organizations culture medium |
| CN103755461A (en) * | 2013-12-23 | 2014-04-30 | 佛山市顺德区今日景艺生物科技有限公司 | Culture medium for culturing orchid tissue and culture method thereof |
| CN103755461B (en) * | 2013-12-23 | 2015-09-16 | 佛山市顺德区今日景艺生物科技有限公司 | The medium that Orchid Tissue is cultivated and cultural method thereof |
| CN103734008A (en) * | 2013-12-26 | 2014-04-23 | 柳州赛特生物科技研发中心 | Liquid culture medium for tissue culture intermediate propagation of phalaenopsis and corresponding culture method |
| CN103734008B (en) * | 2013-12-26 | 2016-04-13 | 广西森仙源生物科技有限公司 | A kind of butterfly orchid tissue cultivating Fast-propagation liquid nutrient medium and corresponding cultural method |
| CN104082148B (en) * | 2014-07-18 | 2016-08-17 | 内蒙古农业大学 | The method of iris aseptic bennet regeneration expanding propagation |
| CN104082148A (en) * | 2014-07-18 | 2014-10-08 | 内蒙古农业大学 | Method for performing regeneration propagation on sterile stalks of butterfly orchids |
| CN104067943A (en) * | 2014-07-18 | 2014-10-01 | 内蒙古农业大学 | Phalaenopsis sterile root propagation method |
| CN104255494A (en) * | 2014-09-19 | 2015-01-07 | 郎溪庆林生态特色农业观光园有限公司 | Culture medium for butterfly orchid tissue culture |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN106922432A (en) * | 2017-01-23 | 2017-07-07 | 柳州市方艺种养场 | A kind of orchid cultivation method |
| CN106922512A (en) * | 2017-03-23 | 2017-07-07 | 苏州农业职业技术学院 | A kind of orchid cultural method |
| CN108077010A (en) * | 2017-12-15 | 2018-05-29 | 佛山科学技术学院 | A kind of hormone treatment method for improving early blossoming chalk Flowering quality |
| CN108419676A (en) * | 2018-03-30 | 2018-08-21 | 内蒙古自治区生物技术研究院 | Iris tissue culture medium (TCM) and preparation method thereof |
| CN109349105A (en) * | 2018-10-10 | 2019-02-19 | 广西生态工程职业技术学院 | A kind of iris tissue-cultured seedling mating system |
| CN109392711A (en) * | 2018-11-20 | 2019-03-01 | 广东省农业科学院作物研究所 | A kind of fast breeding method of glycan seedling |
| CN110036910A (en) * | 2019-04-15 | 2019-07-23 | 中国医学科学院药用植物研究所海南分所 | A kind of blood aspidistra quick breeding by group culture method |
| CN110463556A (en) * | 2019-09-19 | 2019-11-19 | 漳州新镇宇生物科技有限公司 | A kind of iris bottle seedling refinery method |
| CN110896856A (en) * | 2019-11-07 | 2020-03-24 | 郑州师范学院 | Method for inducing complete inflorescence in vitro by axillary bud of inflorescence shaft of phalaenopsis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1258321C (en) | 2006-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1258321C (en) | Quick breeding method for butterfly orchid high quality sprout | |
| CN102870680B (en) | Efficient rapid propagation technique appropriate for detoxified rabbiteye blueberries | |
| CN102301952B (en) | Method for breeding chamomile | |
| CN101647392B (en) | Tissue-culture rapid-propagation method of double-happiness cuckoo variety and special culture medium thereof | |
| CN105104203A (en) | Efficient propagation method for African daisy virus-free seedlings | |
| CN103380730A (en) | Tissue-culture rapid propagation method for pyrus betulaefolia bunge | |
| CN102657088A (en) | Tissue culture method for Ormosia hosiei et Wils | |
| CN103222425A (en) | Efficient and rapid propagation technology suitable for southern highbush blueberry | |
| CN105104202A (en) | Germplasm preservation method for African daisy tissue culture and propagation | |
| CN1284448C (en) | High quality germchit tissure culturing and rapid breeding method of Dendrobium sp. | |
| CN102217551A (en) | Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips | |
| CN101637123B (en) | Organic-culturing quick-reproducing method of curcuma | |
| CN101766123B (en) | Method for rapid propagation of zephyr lily | |
| CN106069748A (en) | The commercial tissue culture of sprout mating system of American red-maple splendidness in October | |
| CN1631108A (en) | High quality germchit rapid breeding method of OncidiumLuridum | |
| CN108391591B (en) | Tissue culture and rapid propagation method for tabebuia flavedo | |
| CN103931499B (en) | A kind of quick breeding method for tissue culture of callistemon rigidus | |
| CN109156350A (en) | A kind of method of the numerous bud of wind resistance paulownia and root media and promotion wind resistance paulownia Vitro Quick Reproduction | |
| CN1248571C (en) | Tamarix chinensis arboriculture technique | |
| CN102499087B (en) | Isolated culture and plant regeneration method of silver chain | |
| CN107047203A (en) | A kind of trident maple cuttage seeding cultivating method | |
| CN107466859A (en) | The rapid propagation method of Grevillea banksii R. Br seedling | |
| KR20110064365A (en) | The mass producing method of regenerated plant from the leaf segment of calanthe discolor | |
| CN104054429B (en) | A kind of spring cuckoo seedling-cultivating method | |
| CN1139319C (en) | Young banian in vitro quick propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |