CN1258321C - Quick breeding method for butterfly orchid high quality sprout - Google Patents
Quick breeding method for butterfly orchid high quality sprout Download PDFInfo
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- CN1258321C CN1258321C CN 200410050906 CN200410050906A CN1258321C CN 1258321 C CN1258321 C CN 1258321C CN 200410050906 CN200410050906 CN 200410050906 CN 200410050906 A CN200410050906 A CN 200410050906A CN 1258321 C CN1258321 C CN 1258321C
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- phalaenopsis
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Abstract
The present invention relates to a quick propagation method for high quality germchits of phalaenopsis and a used culture medium. The phalaenopsis is named as butterfly orchid, is one of the widest and the most popular cultivation kinds of an orchid and is called as 'orchid empress'. The phalaenopsis is a very rare indoor flower, and phalaenopsis varieties with a self-production intellectual property are fewer in a mainland of China. The quality of the germchit of produced phalaenopsis is poorer. The phalaenopsis has a present main propagation method that offsprings are separated, and the fine trait of a mother body and the identity of the germchit can not be maintained or a variation coefficient of the germchit can be increased. The present invention uses a peduncle subsection of the phalaenopsis as an explant, and peduncle seedlings are induced by a unique culture medium. Stem tips or stem segments of the peduncle seedlings pullulate gain and proliferate to produce good quality phalaenopsis germchits. The method has the advantages of simple and economical technique, practicality and high application value and provides an effective approach for satisfying the need of a market.
Description
Technical field
The present invention relates to the high quality seedling method for quickly breeding and the employed medium of Moth orchid.
Background technology
Moth orchid (Phalaenopsis) claim phalaenopsis again, is to cultivate one of extensive, the most universal kind in the orchid, is described as " orchid queen ".This platymiscium whole world has more than 40 initial species approximately; there are 6 initial specieses in China; but since people to wild resource over-exploitation; wherein most of wild initial species has been difficult to find now, and the Moth orchid initial species is also classified as one of the world's ten big protective plant species in imminent danger by world's conservation of wild plant tissue.At present, Moth orchid in different poses and with different expressions on the market, large flower and brilliant color is viewed and admired to plant and mostly is crossbreed, and can belong to hybridization formation intergeneric cross kind with other, as hybridizing formation intergeneric cross kind Doritaenopsis (being abbreviated as Dtps.) with Duo Lilan (Doritis).Because the crossbreed florescence is long, pattern is gorgeous, flower appearance gracefulness, and growth potential is strong, is subjected to liking of various countries people deeply, is a kind of very famous and precious indoor flower, and it not only can be made cut-flower but also can potted plantly view and admire, and is all the fashion in China in recent years.The large-scale commercial production of Moth orchid is quite successful on China Taiwan, Japan and other places, all has every year hundreds and thousands of new varieties to put goods on the market and exports.The industry of China mainland Moth orchid is started late, but unit or the company that has many production Moth orchides in all parts of the country now, yet, the Moth orchid seedling of China many from ground import such as Taiwan or Japan or these companies in China mainland production, it is less that China mainland has the butterfly orchid variety of self-produced intellectual property, and the Moth orchid seedling quality of production is relatively poor.
Moth orchid belongs to single stem aerial orchid, and lateral bud is seldom grown, and the division propagation coefficient is extremely low.Its main propagation method adopts aseptic seeding and passes through evoking adventive bud breeding again such as protocorm, callus.But when high-quality Moth orchid crossbreed utilized the aseptic seeding breeding, the offspring can separate, and can not keep the homogeneity of the merit and the seedling of parent.And adopt protocorm, callus etc. again during evoking adventive bud breeding seedling, can increase the coefficient of variation of seedling.The present invention is an explant with the bennet sections of Moth orchid, utilize unique medium to induce the bennet seedling, be propagation with the stem apex of bennet seedling or the stem section bud of growing thickly again, produce the Moth orchid seedling of high-quality, for the needs that satisfy market provide a valid approach.
At present, the aseptic seeding and the tissue culture of Moth orchid have been carried out more research both at home and abroad, but the report that carries out quick breeding of Moth orchid high quality seedling and large-scale production by the bud approach of growing thickly has not been arranged.
Summary of the invention
Aseptic seeding and tissue culture are often adopted in the breeding of Moth orchid, but aseptic seeding and can not preserve parent's merit when breeding with the protocorm approach.The objective of the invention is by the bennet sections with Moth orchid is explant, utilize unique medium to induce the bennet seedling, carry out adventitious buds proliferation with the stem apex or the stem section of bennet seedling again, produce the Moth orchid seedling of high-quality, carry out the cultivation of knitting of Moth orchid improved seeds with the bud approach of growing thickly, obtain the stable test-tube plantlet of a large amount of proterties, for the needs that satisfy Moth orchid high quality seedling market provide a valid approach, the present invention also provides grow thickly bud tissue culture propagation and the employed medium of multiple Moth orchid hybrid, reaches the selection to explant.
Moth orchid high quality seedling method for quickly breeding of the present invention comprises the steps:
1. material: the used material of the present invention has that red over the sky (Dtps.Queen Beer " Red Sky ", super red (Phalaenopsis Super-Red) etc., material is all taken from the South China Botanical Garden.
2. the sterilization of explant: the bennet of getting the Moth orchid bud, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, again with 0.1% mercuric chloride solution sterilization 15 minutes, behind the aseptic water washing 5 times, blot with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture.
3. inducing of bennet seedling: the inducing culture of bennet seedling is VW+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre, left and right sides sleeping bud started growth in 15 days, when cultivation temperature (20 ± 2) ℃, develop into bud, multiform becomes the bennet seedling of band leaf when cultivation temperature (28 ± 2) ℃, medium contains sucrose 15 grams per liters, pH5.5-5.8, agar 0.7%, illuminance 1500～2000lx, illumination 12 hours/day.Add sodium citrate energy inhibition of browning, improve the growth rate of bennet seedling.
4. adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling, cut off partial blade and transfer on adventitious buds proliferation medium MS+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre+15% Sucus Cocois+sucrose 30 grams per liter medium the formation of can induced bundle sprouting, Sucus Cocois by the juice in the coconut of fresh listing after filtration, add coconut milk and can obviously improve sprouting and growth rate of the bud of growing thickly, the indefinite bud clump that induces is divided into one clump of single bud or several bud, is seeded on the same medium and breeds.If have protocorm to form in the breeding, need to reduce the concentration of 6-benzyl purine.
5. culture of rootage: adventitious buds proliferation is after many generations, the bud of growing thickly can be cut into single bud, be seeded in to contain on the root media of spending precious (Hyponex 1) 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+potato 50-100 grams per liter+activated carbon 2 grams per liters and carry out culture of rootage, can form plant height 3-4 centimetre whole plant about 40 days, adding active carbon, to help plant strain growth sturdy and can prevent brown stain.
6. test-tube seedling transplanting: spring and autumn the favorable season for transplanting.During test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity, and the survival rate of transplanting all can reach 98%-100%.
The Moth orchid seedling that the present invention produces has the genetic stability height, and it is fast to emerge, and seedling is strong, advantages such as seedling quality better, resistance, well-grown.The present invention utilizes the bennet of Moth orchid to be explant, and the unique medium of employing carries out adventitious buds proliferation and obtains the method for high quality seedling, and technology is material benefit simply, and is practical, the using value height.Moth orchid is to cultivate one of extensive, the most universal kind in the orchid, is described as " orchid queen ".Its crossbreed has features such as the florescence is long, and pattern is gorgeous, spends the appearance gracefulness, and growth potential is strong, is subjected to liking of various countries people deeply, is a kind of very famous and precious indoor flower, and it not only can be made cut-flower but also can potted plantly view and admire, and is all the fashion in China in recent years, and the plantation amount is big.It is less that China mainland has the butterfly orchid variety of self-produced intellectual property, and the Moth orchid seedling quality of production is relatively poor.If produce the Moth orchid seedling of high-quality, market prospects are wide.
Embodiment
Embodiment 1
1. draw materials: butterfly orchid variety sunset (Phalaenopsis Taida Salu " sunset ")
2. the sterilization of explant: the bennet of getting the sunset bud, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, used 0.1% (percentage by weight) mercuric chloride solution sterilization again 15 minutes, and behind the aseptic water washing 5 times, blotted with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture.
3. inducing of bennet seedling: the inducing culture of bennet seedling is VW+6-benzyl purine 10 mg/litre+methyl 0.5 mg/litre+sodium citrate 150 mg/litre, and sleeping bud started growth in 15 days, and multiform becomes the bennet seedling of band leaf when cultivation temperature (28 ± 2) ℃.
4. adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling, cut off partial blade and transfer on adventitious buds proliferation medium MS+6-benzyl purine 10 mg/litre+methyl 0.5 mg/litre+150 mg/litre sodium citrate+15% Sucus Cocois+sucrose 30 grams per liter medium the formation of can induced bundle sprouting, the indefinite bud clump that induces is divided into one clump of single bud or several bud, is seeded on the same medium and breeds.
5. culture of rootage: adventitious buds proliferation is after many generations, the bud of growing thickly can be cut into single bud, be seeded in to contain on the root media of spending precious (Hyponex 1) 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+potato 100 grams per liters+activated carbon 2 grams per liters and carry out culture of rootage, can form plant height 3-4 centimetre whole plant about 40 days.
6. test-tube seedling transplanting: spring and autumn the favorable season for transplanting.During test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity, and the survival rate of transplanting all can reach 98%-100%.
Embodiment 2
Taking from the main high butterfly orchid variety of breeding super red (Dtps Super-Red) of ornamental value is explant, and the basic operation method is with embodiment 1 unanimity.But medium component is slightly different.The inducing culture of its bennet seedling is VW+6-benzyl purine 15 mg/litre+methyl 1.0 mg/litre+sodium citrate 100 mg/litre, the adventitious buds proliferation medium is MS+6-benzyl purine 15 mg/litre+methyl 0 mg/litre+sodium citrate 100 mg/litre+15% Sucus Cocois+sucrose 30 grams per liters, and the survival rate of test-tube seedling transplanting can reach 100%.
Claims (4)
1, a kind of Moth orchid high quality seedling method for quickly breeding is characterized in that the bennet sections with Moth orchid is an explant, utilizes unique medium to induce the bennet seedling, is propagation with the stem apex of bennet seedling or the stem section bud of growing thickly again, and concrete steps comprise as follows:
1), the sterilization of explant: the bennet of getting the Moth orchid bud, after rinsing well with running water, clip has sections 2-3 centimetre of sleeping bud, on superclean bench with 70% alcohol-pickled 1 minute, again with percentage by weight 0.1% mercuric chloride solution sterilization 15 minutes, behind the aseptic water washing 5 times, blot with aseptic blotting paper, cut off each 0.5 centimetre at otch two ends, the segment of being cut into long 1-2 centimetre of band sleeping bud is seeded on the bennet seedling inducing culture;
2), inducing of bennet seedling: the bennet seedling is on inducing culture, left and right sides sleeping bud started growth in 15 days, when 20 ± 2 ℃ of cultivation temperature, developed into bud, when 28 ± 2 ℃ of cultivation temperature, multiform becomes the bennet seedling of band leaf, illuminance 1500～2000lx, illumination 12 hours/day, the inducing culture of bennet seedling is VW+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre, medium contains sucrose 15 grams per liters, pH 5.5-5.8, agar 0.7%;
3), adventitious buds proliferation: when cultivating 40 days, 1-2 centimetre of bennet height of seedling, cutting off partial blade transfers on the adventitious buds proliferation medium, the formation of can induced bundle sprouting, the indefinite bud clump that induces is divided into one clump of single bud or several bud, be seeded on the same medium and breed, the adventitious buds proliferation medium is MS+6-benzyl purine 5-20 mg/litre+methyl 0.5-1.0 mg/litre+sodium citrate 50-200 mg/litre+15% Sucus Cocois+sucrose 30 grams per liters;
4), culture of rootage: adventitious buds proliferation is after many generations, the bud of growing thickly can be cut into single bud, be seeded in and carry out culture of rootage on the root media, can form plant height 3-4 centimetre whole plant in 40 days, root media is spent precious 3 grams per liters+sucrose 15 grams per liters+bananas juice 100 grams per liters+potato 50-100 grams per liter+activated carbon 2 grams per liters for containing;
5), test-tube seedling transplanting: during test-tube seedling transplanting, the Moth orchid seedling is taken out from vial, clean the medium of root, in 0.1% liquor potassic permanganate, soak airing after 5 minutes, with little plastic basin is cultivating container, with the fresh fountain moss of soaking is cultivation matrix, keeps suitably ventilating and enough humidity.
2,, it is characterized in that described Sucus Cocois by the juice in the fresh coconut after filtration according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1.
3, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, it is characterized in that 5) described in test-tube plantlet be implanted in spring and autumn favorable season for transplanting.
4, according to the Moth orchid high quality seedling method for quickly breeding described in the claim 1, the kind that it is characterized in that described Moth orchid is for red, super red over the sky.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410050906 CN1258321C (en) | 2004-07-30 | 2004-07-30 | Quick breeding method for butterfly orchid high quality sprout |
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| CN 200410050906 CN1258321C (en) | 2004-07-30 | 2004-07-30 | Quick breeding method for butterfly orchid high quality sprout |
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| CN1586165A CN1586165A (en) | 2005-03-02 |
| CN1258321C true CN1258321C (en) | 2006-06-07 |
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- 2004-07-30 CN CN 200410050906 patent/CN1258321C/en not_active Expired - Fee Related
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