CN109349105A - A kind of iris tissue-cultured seedling mating system - Google Patents
A kind of iris tissue-cultured seedling mating system Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of iris tissue-cultured seedling mating systems, method includes the following steps: 1. iris bennet segment band resting bud is seeded on inducing clumping bud culture medium；2. being seeded in proliferated culture medium after Multiple Buds are cut, it is transferred under nature radiant and cultivates after LED red-light source culture；3. Multiple Buds are divided into simple bud to be seeded in ape xification culture medium, it is placed under LED composite light source and cultivates；4. moving on to natural light hardening in the greenhouse with sunshade net；It is cultivated 5. being wrapped up iris seedling root system with sphagna and being placed in greenhouse；6. starting to apply fertilizer when thering is new root to grow；7. root system changes 3.5 cun of nutritive cubes into when coiling full in sphagna；8. fertilising in every 10 days is primary after changing basin 10 days.This method breeds iris tissue-cultured seedling, can effectively overcome that adventitious buds proliferation rate is low, rooting rate is low and transplanting back root part is perishable that Phalaenopsis plants is led to problems such as to turn to be yellow, while iris tissue-cultured seedling production cost can be reduced.
Description
Technical field
The invention belongs to flower plants and nursery stocks to breed field, especially a kind of iris tissue-cultured seedling mating system.
Background technique
Phalaenopsis seldom develops side shoot in single stem aerial orchid, it is difficult to carry out conventional vegetative propagation；In addition iris
It cannot complete to pollinate under field conditions (factors), hardly result in seed, even if carrying out artificial pollination obtains seed, be free of embryo in seed
Cream is also difficult to sprout under natural conditions, and germination percentage is extremely low, therefore iris is difficult to be utilized seed and carries out sexual propagation.It is main at present
The quick breeding of iris is carried out using method for tissue culture, the tissue cultures of iris can obtain a large amount of excellent in a short time
Matter, neat iris young plant, have that proliferation rate is high, speed is fast, be not subject to seasonal restrictions, whole year production and can easily remove
The advantages that viral is the high effective way of plant modification.
But existing iris tissue-cultured seedling breeding has the following problems: first, iris is using approach such as callus
When evoking adventive bud is bred, character variation can occur for offspring, lead to the merit for not being able to maintain parent.Second, traditional butterfly
Phalaenopsis tissue culture light source is white fluorescent lamp, and calorific value is big, largely uses fluorescent lamp as light source, increases the control of room temperature
This is made；And light utilization efficiency is low, wastes resource significantly.Third, white fluorescent lamp is as iris tissue culture light source, Multiple Buds
Proliferation rate and quantity of taking root are lower.4th, iris tissue-cultured seedling be enclosed sterile, nutrition supply is abundant, illumination is suitable, temperature
It is grown in degree and the humidity environment of perseverance, when bottle outlet transplanting front and back environmental condition gap is larger, test tube seedling is to new environment
Adaptability is poor, causes transplanting survival rate low.5th, cultivation matrix is also one for influencing the large-scale production of iris tissue-cultured seedling
Key factor.At present using Chilean import sphagna as the Main Cultivation matrix of iris tissue-cultured seedling, Chilean import sphagna can be preferable
The gas permeability for solving the problems, such as cultivation matrix, but increase with the time is cultivated, the simple cultivation base constituted with Chilean import sphagna
Matter will appear acidification and the phenomenon that addles, and easily cause iris tissue-cultured seedling root rot；Simultaneously as Chilean import sphagna price one
Road is risen, so that iris single plant seeling industry cost price is high.
Summary of the invention
The purpose of the present invention: being to provide a kind of iris tissue-cultured seedling mating system, and iris tissue-culture container seedling is made to keep excellent
Benign shape promotes light source utilization efficiency, improves adventitious buds proliferation rate and rooting rate, increases iris tissue-cultured seedling to environment after transplanting
Adaptability, filter out the cultivation matrix of more conducively iris tissue-cultured seedling plant modification, finally obtain a kind of iris group
Train height of seedling effect, the mating system of low cost.
In order to achieve the above objectives, it the technical scheme is that a kind of iris tissue-cultured seedling mating system, including walks as follows
It is rapid:
(1) using the bennet segment of iris as explant, long 1-2 centimetres cutting with resting bud inducing clumping bud: is cut into after disinfection
Section is seeded on inducing clumping bud culture medium；The first dark culture in the induced medium；It moves under nature radiant and cultivates again,
Obtain Multiple Buds；
(2) adventitious buds proliferation: the Multiple Buds that step (1) is obtained, cutting is divided into simple bud or cluster bud is seeded in proliferated culture medium,
And place it in be transferred under nature radiant after culture under LED red-light source and cultivate, obtain the Multiple Buds of proliferation of propagation；
(3) ape xification: the Multiple Buds of step (2) proliferation of propagation are divided into simple bud and are seeded in ape xification culture medium, by it
It is placed under LED composite light source and cultivates, obtain iris tissue-cultured seedling；
(4) hardening: when the iris tissue-cultured seedling base portion that step (3) obtains has the 2-3 tip of a root, the greenhouse with sunshade net is moved on to
Interior hardening, the tissue-cultured seedling after obtaining hardening；
(5) iris tissue culture transplantation of seedlings: the tissue-cultured seedling after step (4) hardening grows 3-4 piece leaf, 2-4 item root, root long 2-3cm
When, iris seedling is taken out from vial, cleans the culture medium of root, impregnates iris seedling root with carbendazim solution
Afterwards, then with the sphagna that sterilized iris seedling root system is wrapped up, be placed in 1.5 cun of nutritive cubes, and with Masson Pine Bark fragment, mud
The first matrix that charcoal soil and coco bran mix fills up nutritive cube, is placed in the greenhouse with sunshade net and cultivates；
(6) apply fertilizer after transplanting: iris tissue-cultured seedling starts to apply the first compound fertilizer when having new root to grow；
(7) iris tissue-cultured seedling changes basin: the root system to iris tissue-cultured seedling in (6) 1.5 cun of nutritive cubes of step coils in sphagna
Man Shi, the Masson Pine Bark block 3-4cm after 3.5 cun of nutritive cube bottom pad fermentations is thick, is put into after having shelled 1. 5 cun of nutritive cubes
Iris tissue-cultured seedling, then nutritive cube is filled up with the second matrix that Masson Pine Bark block, peat soil and coco bran mix；Continue to put
It is cultivated in the greenhouse with sunshade net；
(8) apply fertilizer after changing basin: after step (7) iris tissue-cultured seedling changes basin 10 days, natural lighting is enhanced to intensity of illumination and is
10000-15000lux applies the second compound fertilizer；
Complete the breeding of iris tissue-cultured seedling.
Preferably, the inducing clumping bud culture medium as described in step (1) is MS+6-BA(6- benzyl aminoadenine)
3mg/L+NAA(methyl α-naphthyl acetate) 0.2mg/L+ sucrose 20g/L+ coconut juice 100mL/L+ agar 6.0 g/L, pH value 5.6-6.0.
Preferably, step (1) temperature is 25 ± 2 DEG C, is cultivated 5-10 days；Step (1) the natural light light
Incubation time is 15-20 days, intensity of illumination 1500-2000lux, light application time 12h/d under source.
Preferably, adventitious buds proliferation culture medium described in the step (2) is MS+KT(kinetin) 1.0mg/L+6-
BA 2.0mg/L+NAA 0.5mg/L+ coconut juice 100mL/L+ sucrose 30g/L+agar 6.0g/L, pH value 5.6-6.0.
Preferably, the wavelength of LED red-light source described in the step (2) is 630nm, light application time 12h/d, training
Supporting the time is 5-10 days.
Preferably, the intensity of illumination cultivated under natural radiant in the step (2) is 1500-2000lux, light application time
For 12h/d, incubation time is 20-30 days.
Preferably, ape xification culture medium described in the step (3) is 1/4MS+NAA 0.1mg/L+ banana puree
30g/L+ mashed potatoes 40g/L+ sucrose 5g/L+ agar 6.0g/L, pH value 5.6-6.0.The effect of ape xification culture medium is to lure
Lead iris tissue culture seedling rooting.
Preferably, LED composite light source described in the step (3) is that two kinds of monochromatic light of LED feux rouges and LED blue light are combined into
LED composite light source, wherein the wavelength of red-light LED is 630nm；The wavelength of blue-ray LED is 450-480nm, feux rouges and blue light proportion
R:B(light quantity ratio)=5:1.
Preferably, the intensity of illumination cultivated under step (3) the LED composite light source is 1500-2000lux, light application time
For 12h/d, incubation time is 15-20 days.
Preferably, step (4) the hardening light source is natural light, and the control of hardening temperature is in 16-30 DEG C, intensity of illumination
4000-8000lux, relative air humidity 70%-90%, hardening time are 20-30 days.
Preferably, Masson Pine Bark fragment described in the step (5), a length of 1-2cm, width 0.5-1cm pass through
PH value is 5.5-6.5 after decomposed, and EC value is 0.2-0.4ms/cm；
First matrix described in the step (5) includes: 60-70 parts of Masson Pine Bark fragment by weight, 15-20 parts
Peat soil, 15-20 parts of coco bran；
In the step (5), after being impregnated iris seedling root 30-60 seconds with 500-1000 times of carbendazim solution, with disinfection
The sphagna package iris seedling root system crossed, is placed in 1.5 cun of nutritive cubes, and with Masson Pine Bark fragment, peat soil and coco bran
The first matrix mixed fills up nutritive cube, is placed in the greenhouse with sunshade net and cultivates, 22-28 DEG C of cultivation temperature, natural
Light illumination, intensity of illumination 4000-8000lux, relative air humidity 70%-90%.
Preferably, the Masson Pine Bark block after fermentation described in the step (7), a length of 3-8cm, width 1-3cm,
Make pH value 5.5-6.5, EC value 0.2-0.4ms/cm after decomposed；Second matrix described in the step (7) is by weight
It include: 40-48 parts of Masson Pine Bark block, 26-30 parts of peat soil, 26-30 parts of coco bran.
Preferably, it is 20-30 DEG C that condition of culture, which is cultivation temperature, in the greenhouse of the step (7), natural lighting, illumination
Intensity is 8000-10000lux, relative air humidity 60%-80%.
Preferably, in the step (6) the first compound fertilizer be dilute 4000 times N:P:K=10:30:20 compound fertilizer, often
It applies within 15 days primary；(7) second compound fertilizer of step be apply dilution 1000 times N:P:K=25:10:10 compound fertilizer, every 10 days
It applies primary.
The present invention has the beneficial effect that:
1. the present invention uses the bennet segment of iris for explant, directly formation Multiple Buds are protected without callus phase
The merit of parent is held.
2. having used natural radiant in three phases such as inducing clumping bud, adventitious buds proliferation, hardenings, it is therefore an objective to improve
Iris tissue-cultured seedling carries out photosynthetic ability using nature radiant, significantly increases to the adaptability of natural lighting,
Production cost is reduced simultaneously.
3. LED light source has been used in the adventitious buds proliferation stage, to improve the proliferation rate of iris tissue-cultured seedling.
4. LED composite light source has been used in the ape xification stage, to improve the tip of a root quantity of iris tissue-cultured seedling.
5. the use of 1/4MS being that iris tissue-cultured seedling is made quickly to form the tip of a root in the ape xification stage；And it is used cooperatively simultaneously
5g/L sucrose (is far below normal sucrose concentration 30g/L), it is therefore an objective to and it is only short-term to provide carbon source for the growth of iris tissue-cultured seedling, it is refining
The seedling stage can make iris tissue-cultured seedling that natural light more be used to carry out photosynthesis, provide the carbon source of volunteer growth.
6. Masson Pine Bark, peat soil and coco bran etc. are mixed in proportion as cultivation base in terms of transplanting medium
Matter, the Masson Pine Bark content of organic matter is abundant, and cultivation matrix can be made to keep higher gas permeability, promotes iris tissue-cultured seedling root
System's growth, effectively inhibits the generation of iris root rot；Furthermore use Masson Pine Bark and coco bran opposite as the cost of matrix
Import sphagna wants much lower, and production cost can be effectively reduced.
Specific embodiment
Below with reference to embodiment, the present invention will be further described.
Embodiment 1: it was carried out in 2016 in one iris tissue-cultured seedling Breeding base of Liuzhou Liubei District, is with iris
" big capsicum " kind is embodiment.Specific step is as follows:
(1) inducing clumping bud: using the bennet segment of " big capsicum " iris as explant, it is cut into long 1-2 centimetres of band after disinfection and stops
The dissection of dormancy bud is seeded on inducing clumping bud culture medium, and inducing clumping bud culture medium is MS+6-BA 3mg/L+NAA 0.2mg/
L+ sucrose 20g/L+ coconut juice 100mL/L+ agar 6.0g/L, pH value 5.6-6.0；The first dark culture 7 in the induced medium
It, cultivation temperature is 25 ± 2 DEG C；It moves under nature radiant and cultivates 18 days, intensity of illumination 1500-2000lux again, when illumination
Between be 12h/d；
(2) adventitious buds proliferation: after inducing clumping bud 25 days, at 1-2 centimetres of height of seedling, Multiple Buds cutting is divided into simple bud or cluster bud connects
For kind into proliferated culture medium, adventitious buds proliferation culture medium is MS+KT 1.0mg/L+6-BA 2.0mg/L+NAA 0.5mg/L+ coconut palm
Juice 100mL/L+ sucrose 30g/L+agar 6.0g/L, pH value 5.6-6.0, and place it in the LED feux rouges that wavelength is 630nm
It is cultivated under light source, light application time 12h/d, cultivation temperature is 25 ± 2 DEG C；The culture of LED red-light source was transferred to natural light light after 7 days
It is cultivated under source, cultivation temperature is 25 ± 2 DEG C, intensity of illumination 1500-2000lux, light application time 12h/d, natural radiant
Lower culture 23 days；
(3) ape xification: the Multiple Buds that proliferation of propagation is obtained are divided into simple bud and are seeded in ape xification culture medium, ape xification
Culture medium is 1/4MS+NAA 0.1mg/L+ banana puree 30g/L+ mashed potatoes 40g/L+ sucrose 5g/L+ agar 6.0g/L, pH
Value 5.6-6.0 is placed it under LED composite light source and is cultivated, and LED composite light source is by two kinds of monochromatic light of LED feux rouges and LED blue light
It is combined into, wherein the wavelength of red-light LED is 630nm, and the wavelength of blue-ray LED is 450-480nm, and feux rouges and blue light match R:B(light
Amount ratio)=5:1, cultivation temperature is 25 ± 2 DEG C, intensity of illumination 1500-2000lux, light application time 12h/d, multiple in LED
It is cultivated 15 days under light combination source；
(4) when iris tissue-cultured seedling base portion has the 2-3 tip of a root, hardening in the greenhouse with sunshade net, hardening light hardening: are moved on to
Source is natural light, and hardening temperature controls within the scope of 16-30 DEG C, intensity of illumination 4000-8000lux, and relative air humidity is
70%-90%, the hardening time are 25 days；
(5) iris tissue culture transplantation of seedlings: growing 3-4 piece leaf to tissue-cultured seedling, 2-4 item root, when root long about 2-3cm, iris is small
Seedling takes out from vial, cleans the culture medium of root, impregnates iris seedling root with 500-1000 times of carbendazim solution
After 30-60 seconds, iris seedling root system is wrapped up with the sphagna sterilized, is placed in 1.5 cun of nutritive cubes；And with the size after decomposed
The matrix mixed for Masson Pine Bark fragment, peat soil and the coco bran of 1-2cm(long) × 0.5-1cm(wide) fills up nutrition
Alms bowl；The mixed-matrix is to be according to mass percent meter, 65 parts of Masson Pine Bark fragment, 15 parts of peat soil, 20 parts of coconut palm
Chaff mixes；1.5 cun of nutritive cubes are filled up after matrix to be placed in the greenhouse with sunshade net and be cultivated, and cultivation temperature is 22-28 DEG C,
Natural lighting, intensity of illumination 4000-8000lux, relative air humidity 70%-90%；
(6) apply fertilizer after transplanting: iris tissue-cultured seedling starts to apply fertilizer when having new root to grow, and applies N:P:K=10:30 of 4000 times of dilution:
20 compound fertilizer applies primary for every 15 days；
(7) iris tissue-cultured seedling changes basin: when the root system of 1.5 cun of seedlings coils full in sphagna, in 3.5 cun of nutritive cube bottom pad hairs
Masson Pine Bark block about 3-4cm after ferment is thick, is put into the iris tissue-cultured seedling after having shelled 1. 5 cun of nutritive cubes, place into it is decomposed after
Size be 3-8cm(long) × 1-3cm(wide) and the matrix that mixes of Masson Pine Bark block, peat soil and coco bran fill up nutrition
Alms bowl, the mixed-matrix are to be according to mass percent meter, 45 parts of Masson Pine Bark block, 27 parts of peat soil, 28 parts of coco bran
It mixes；3.5 cun of nutritive cubes are filled up after matrix to continue to be placed in the greenhouse with sunshade net and be cultivated, cultivation temperature 20-30
DEG C, natural lighting, intensity of illumination 8000-10000lux, relative air humidity 60%-80%；
(8) apply fertilizer after changing basin: after iris tissue-cultured seedling changes basin 10 days, illumination is gradually increased, intensity of illumination 10000-
15000lux applies the compound fertilizer of N:P:K=25:10:10 of 1000 times of dilution, applies within every 10 days primary.
This method is used alternatingly using LED light source and natural light, 10,000 plants of " big capsicum " iris tissue-cultured seedling is produced, in clump
Sprout induction period, adventitious buds proliferation stage and ape xification stage consume altogether electricity be 3200 degree；And use ordinary white glimmering
Light source of the light lamp as inducing clumping bud stage, adventitious buds proliferation stage and ape xification stage produces 10,000 plants of " big capsicum " butterflies
Consumption electricity is 4500 degree to phalaenopsis tissue-cultured seedling altogether at this stage.Wherein, air-conditioning saves 900 degree of electricity, and illumination saves electricity and saves 400
Degree adds up to and saves 1300 degree of electricity.As it can be seen that being used alternatingly using LED light source and natural light, " big capsicum " iris group can be reduced
Train the electric cost of seedling.
This method is used alternatingly using LED light source and natural light, and " big capsicum " iris adventitious buds proliferation rate is 3.2 times；
And ordinary white fluorescent lamp is used, " big capsicum " iris adventitious buds proliferation rate is 2.9 times.Root is induced using LED composite light source
Point simultaneously uses natural light hardening, and " big capsicum " iris is taken root quantity average out to 3.5；And ordinary white fluorescent lamp is used, it is " big
Capsicum " iris is taken root quantity average out to 3.1.As it can be seen that being used alternatingly using LED light source and natural light, can improve " big peppery
The adventitious buds proliferation rate of green pepper " iris tissue-cultured seedling and quantity of taking root.
This method makes " big capsicum " iris tissue-cultured seedling in inducing clumping bud stage, adventitious buds proliferation stage using LED light
Source and natural light are used alternatingly, and are put into greenhouse using natural light hardening, in the hardening stage after LED composite light source induces the tip of a root
Reach hardening progress synchronous with root growth is promoted, " big capsicum " Transplantation Survival Rate of Phalaenopsis hybrimycin Tissue Culture Seedlings that the method is cultivated reaches
98.2%；And inducing clumping bud stage, adventitious buds proliferation stage and the stage of taking root use ordinary white fluorescent lamp as light source, and
It is carried out in tissue culture room, " big capsicum " Transplantation Survival Rate of Phalaenopsis hybrimycin Tissue Culture Seedlings of cultivation only 87.1%.As it can be seen that being lured in Multiple Buds
Lead the stage, the adventitious buds proliferation stage is used alternatingly using LED light source and natural light, and put after LED composite light source induces the tip of a root
Enter greenhouse using natural light hardening, makes hardening is synchronous with root growth is promoted to carry out in the hardening stage, " big capsicum " butterfly can be improved
Blue tissue-cultured seedling transplanting survival rate.
This method wraps up " big capsicum " iris seedling root system with sphagna, and is placed in 1.5 cun of nutritive cubes；And with after decomposed
Size be 1-2cm(long) × 0.5-1cm(wide) the matrix conduct that mixes of Masson Pine Bark fragment, peat soil and coco bran
" big capsicum " iris tissue-cultured seedling cultivation matrix；And used in 3.5 cun of nutritive cubes it is decomposed after size be 3-8cm(long) ×
1-3cm(wide) the matrix that mixes of Masson Pine Bark block, peat soil and coco bran planted as " big capsicum " iris tissue-cultured seedling
Matrix is trained, is bloomed after withering in " big capsicum " iris tissue-cultured seedling, statistics " big capsicum " iris tissue-cultured seedling root rot rate is only
0.8%, and root rot is slight；Furthermore the matrix cost of single plant " big capsicum " iris is 0.12 yuan.And all using water
It when tongue fur is as " big capsicum " iris tissue-cultured seedling cultivation matrix, blooms after withering, counts " big capsicum " in " big capsicum " iris
Iris root rot rate be 11.7%, wherein have 18% it is withered because of root rot；Furthermore the matrix of single plant " big capsicum " iris
Cost is 0.17 yuan.As it can be seen that transplanting medium of the invention, can significantly reduce the root rot of " big capsicum " iris tissue-cultured seedling, together
When also reduce culture substrate cost.
The preferred embodiment of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, several deformations can also be made, improves and substitutes, these belong to this hair
Bright protection scope.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of iris tissue-cultured seedling mating system, which comprises the steps of:
(1) using the bennet segment of iris as explant, long 1-2 centimetres cutting with resting bud inducing clumping bud: is cut into after disinfection
Section is seeded on inducing clumping bud culture medium；The first dark culture in the induced medium；It moves under nature radiant and cultivates again,
Obtain Multiple Buds；
(2) adventitious buds proliferation: the Multiple Buds that step (1) is obtained, cutting is divided into simple bud or cluster bud is seeded in proliferated culture medium,
And place it in be transferred under nature radiant after culture under LED red-light source and cultivate, obtain the Multiple Buds of proliferation of propagation；
(3) ape xification: the Multiple Buds of step (2) proliferation of propagation are divided into simple bud and are seeded in ape xification culture medium, by it
It is placed under LED composite light source and cultivates, obtain iris tissue-cultured seedling；
(4) hardening: when the iris tissue-cultured seedling base portion that step (3) obtains has the 2-3 tip of a root, the greenhouse with sunshade net is moved on to
Interior hardening, the tissue-cultured seedling after obtaining hardening；
(5) iris tissue culture transplantation of seedlings: the tissue-cultured seedling after step (4) hardening grows 3-4 piece leaf, 2-4 item root, root long 2-3cm
When, iris seedling is taken out from vial, cleans the culture medium of root, impregnates iris seedling root with carbendazim solution
Afterwards, then with the sphagna that sterilized iris seedling root system is wrapped up, be placed in 1.5 cun of nutritive cubes, and with Masson Pine Bark fragment, mud
The first matrix that charcoal soil and coco bran mix fills up nutritive cube, is placed in the greenhouse with sunshade net and cultivates；
(6) apply fertilizer after transplanting: iris tissue-cultured seedling starts to apply the first compound fertilizer when having new root to grow；
(7) iris tissue-cultured seedling changes basin: the root system to iris tissue-cultured seedling in (6) 1.5 cun of nutritive cubes of step coils in sphagna
Man Shi, the Masson Pine Bark block 3-4cm after 3.5 cun of nutritive cube bottom pad fermentations is thick, is put into after having shelled 1. 5 cun of nutritive cubes
Iris tissue-cultured seedling, then nutritive cube is filled up with the second matrix that Masson Pine Bark block, peat soil and coco bran mix；Continue to put
It is cultivated in the greenhouse with sunshade net；
(8) apply fertilizer after changing basin: after step (7) iris tissue-cultured seedling changes basin 10 days, natural lighting is enhanced to intensity of illumination and is
10000-15000lux applies the second compound fertilizer；
Complete the breeding of iris tissue-cultured seedling.
2. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (1)
The inducing clumping bud culture medium stated is MS+6-BA 3mg/L+NAA 0.2mg/L+ sucrose 20g/L+ coconut juice 100mL/L+ agar 6.0
G/L, pH value 5.6-6.0；
Step (1) temperature is 25 ± 2 DEG C, is cultivated 5-10 days；Incubation time under the natural radiant of the step (1)
It is 15-20 days, intensity of illumination 1500-2000lux, light application time 12h/d.
3. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (2)
The adventitious buds proliferation culture medium stated is MS+KT 1.0mg/L+6-BA 2.0mg/L+NAA 0.5mg/L+ coconut juice 100mL/L+ sugarcane
Sugared 30g/L+agar 6.0g/L, pH value 5.6-6.0.
4. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (2)
The wavelength for the LED red-light source stated is 630nm, and light application time 12h/d, incubation time is 5-10 days；In the step (2) certainly
The intensity of illumination cultivated under right radiant is 1500-2000lux, and light application time 12h/d, incubation time is 20-30 days.
5. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (3)
The ape xification culture medium stated is 1/4MS+NAA 0.1mg/L+ banana puree 30g/L+ mashed potatoes 40g/L+ sucrose 5g/L+ fine jade
Rouge 6.0g/L, pH value 5.6-6.0.
6. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (3)
The LED composite light source stated is that two kinds of monochromatic light of LED feux rouges and LED blue light are combined into LED composite light source, wherein the wave of red-light LED
A length of 630nm；The wavelength of blue-ray LED is 450-480nm, and feux rouges and blue light match R:B(light quantity ratio)=5:1；The step
(3) intensity of illumination cultivated under LED composite light source is 1500-2000lux, light application time 12h/d, incubation time 15-20
It.
7. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: step (4) hardening
Light source is natural light, and hardening temperature is controlled at 16-30 DEG C, intensity of illumination 4000-8000lux, relative air humidity 70%-
90%, the hardening time is 20-30 days.
8. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (5)
The Masson Pine Bark fragment stated, a length of 1-2cm, width 0.5-1cm, pH value is 5.5-6.5 after decomposed, and EC value is 0.2-
0.4ms/cm；
First matrix described in the step (5) includes: 60-70 parts of Masson Pine Bark fragment by weight, 15-20 parts
Peat soil, 15-20 parts of coco bran；
In the step (5), after being impregnated iris seedling root 30-60 seconds with 500-1000 times of carbendazim solution, with disinfection
The sphagna package iris seedling root system crossed, is placed in 1.5 cun of nutritive cubes, and with Masson Pine Bark fragment, peat soil and coco bran
The first matrix mixed fills up nutritive cube, is placed in the greenhouse with sunshade net and cultivates, 22-28 DEG C of cultivation temperature, natural
Light illumination, intensity of illumination 4000-8000lux, relative air humidity 70%-90%.
9. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: institute in the step (7)
Masson Pine Bark block after the fermentation stated, a length of 3-8cm, width 1-3cm make pH value 5.5-6.5, EC value after decomposed
0.2-0.4ms/cm；Second matrix described in the step (7) includes: 40-48 parts of Masson Pine Bark block by weight,
26-30 parts of peat soil, 26-30 parts of coco bran；
It is 20-30 DEG C that condition of culture, which is cultivation temperature, in the greenhouse of the step (7), natural lighting, intensity of illumination 8000-
10000lux, relative air humidity 60%-80%.
10. a kind of iris tissue-cultured seedling mating system according to claim 1, it is characterised in that: in the step (6)
One compound fertilizer is the compound fertilizer of N:P:K=10:30:20 of 4000 times of dilution, is applied within every 15 days primary；
(7) second compound fertilizer of step is the compound fertilizer for applying N:P:K=25:10:10 of 1000 times of dilution, is applied within every 10 days primary.
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