CN1994068A - Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel - Google Patents
Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel Download PDFInfo
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- CN1994068A CN1994068A CNA200610161523XA CN200610161523A CN1994068A CN 1994068 A CN1994068 A CN 1994068A CN A200610161523X A CNA200610161523X A CN A200610161523XA CN 200610161523 A CN200610161523 A CN 200610161523A CN 1994068 A CN1994068 A CN 1994068A
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- bennet
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- nodal
- butterfly orchid
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Abstract
The invention relates to a method for using bud to induce the stem to cultivate iris, wherein it comprises that: 1, cultivating the iris until stem; 2, disinfecting the cut stem, cutting into sections with bud, removing package, inserting into cultivate medium; 3, adjusting condition, 4, cultivating in bottle; 5, cutting new stem into section with bud, inserting into cultivate medium, repeating steps 4, 5; repeating steps 5, until there is stem with node bud; 7, cutting stem into section with node bud, inserting into cultivate medium, adjusting condition, inducing bud into nourishment bud; 8, using it as external plant, gaining or separating cultivating; 9, cultivating it on cultivate medium into iris plant.
Description
Technical field
The present invention relates to a kind of method of inducing the bennet propagating quickly butterfly orchid by the bennet nodal bud.
Background technology
Moth orchid (Phalaenopsis) is described as " orchid queen consort ", and high ornamental value and commercial value are arranged.At present after determining improved seeds, generally be after setting up sterile system by bennet, directly the vegetative bud of inducing with bennet is bred, reproduction coefficient generally had only about 2.0 in per two months, especially in the initial culture process, need very very long breeding, have a strong impact on improved seeds and form scale output within a short period of time, thereby lose the first market opportunities.
Summary of the invention
The invention provides and a kind ofly induce the method for bennet propagating quickly butterfly orchid by the bennet nodal bud, it has solved the problem that famous and precious butterfly orchid variety is difficult to form scale output within a short period of time.
The present invention has adopted following technical scheme: a kind ofly induce the method for bennet propagating quickly butterfly orchid by the bennet nodal bud, it adopts following steps: step 1, and will need propagating quickly butterfly orchid to cultivate to extracting bennet out, if take out bennet, directly cut with scalpel; Step 2 after the bennet sterilization that cuts, is cut into the segment with a nodal bud, and peels off the outer luxuriant clothing of nodal bud, presses morphologic principle forward oblique cutting on the suitable culture base; Step 3 is adjusted condition of culture, directly induces nodal bud to be divided into new bennet; Step 4 continues to cultivate bennet in blake bottle; Step 5 with the bennet of newly extracting out, directly is cut into the segment with a nodal bud respectively, on the suitable culture base, repeats above-mentioned steps four, step 5 by morphologic forward oblique cutting; Step 6, repeating step five is until producing band nodal bud bennet; Step 7 is cut into segment with a nodal bud with the bennet that obtains, and oblique cutting is regulated condition of culture on appropriate media, nodal bud is induced be divided into vegetative bud; Step 8 is an explant with the vegetative bud, breeds according to a conventional method or differentiation culture; Step 9 becomes the Moth orchid plantlet with the vegetative bud differentiation culture on the suitable culture base that obtains.
The butterfly orchid variety of described quick breeding need be taken pictures with digital camera, files to preserve parent's image document.The process of the sterilization described in the step 2 is cut a circumference wound for after being stained with 75% alcohol disinfecting with cotton with sharp keen scalpel along the periphery of bennet, fracture with tweezers then.Bennet sections after will rolling over is again inserted in the vial after the sterilization, pours 1.0% liquor natrii hypochloritis sterilization into, with scalpel strip off bract, pours among 0.75% the liquor natrii hypochloritis sterilization again into 5 minutes, takes out with aseptic water washing clean then.Condition of culture described in the step 3 is that temperature condition is at 18 ℃-23 ℃.Bennet needs to be long to 6cm in the step 4.Be cut in the step 5 and look the nodal bud number with the quantity of the segment of a nodal bud and how much decide, can be cut into 5 to 6 segments.Condition of culture in the step 7 is 25 ℃, and illumination is 14001x.
Adopt as above technical scheme, the present invention utilizes the plant physiology principle, make that Moth orchid is fast numerously directly to carry out in the bennet mode, the improved seeds of Moth orchid initial culture during the stage reproduction coefficient bring up to about 5.0-6.0, the initial culture cycle also shortened to about 20 days from two months, be equivalent to efficient and improve 10 times, production cost can reduce by 50% at least, has solved the problem that famous and precious butterfly orchid variety is difficult to form scale output within a short period of time.
Embodiment
The present invention is a kind of method of inducing the bennet propagating quickly butterfly orchid by the bennet nodal bud, at first selecting to need the butterfly orchid variety of breeding fast, take pictures with digital camera, file and preserve parent's image document, carry out following operation then: step 1, to need propagating quickly butterfly orchid to cultivate,, directly cut with scalpel if take out bennet to extracting bennet out; Step 2, the bennet that cuts is stained with 75% alcohol disinfecting with cotton after, cut a circumference wound along the periphery of bennet with sharp keen scalpel, fracture with tweezers then, bennet sections after will rolling over is again inserted in the vial after the sterilization, pour 1.0% liquor natrii hypochloritis sterilization into, with scalpel strip off bract, poured among 0.75% the liquor natrii hypochloritis sterilization again into 5 minutes, clean with aseptic water washing then, be cut into the segment with a nodal bud at about 1-1.5cm place, distance nodal bud two ends, and peel off the outer luxuriant clothing of nodal bud, the forward oblique cutting of pressing the morphology principle is on the suitable culture base; Step 3 is adjusted condition of culture, condition of culture be temperature condition at 18 ℃-23 ℃, directly induce nodal bud to be divided into new bennet; Step 4, in blake bottle, continue to cultivate bennet, the new bennet of extracting out needs to be long to 6cm, step 5 with the bennet of newly extracting out, directly is cut into the segment with a nodal bud respectively, how much look the nodal bud number with the quantity of the segment of a nodal bud decides, generally can be cut into 5 to 6 segments, also press morphologic forward oblique cutting on the suitable culture base, repeat above-mentioned steps four, step 5; Step 6, repeating step five is until the band nodal bud bennet that produces sufficient amount; Step 7 is cut into segment with a nodal bud with the bennet that obtains, and oblique cutting is regulated condition of culture on appropriate media, and condition of culture is 25 ℃, and illumination is 14001x, nodal bud is induced be divided into vegetative bud; Step 8 is an explant with the vegetative bud, breeds according to a conventional method or differentiation culture; Step 9 becomes the Moth orchid plantlet with vegetative bud differentiation culture on the suitable culture base of the sufficient amount that obtains, at last the Moth orchid seedling is carried out normal management, cultivates to the finished product flower.
Claims (7)
1, a kind ofly induce the method for bennet propagating quickly butterfly orchid by the bennet nodal bud, it adopts following steps:
Step 1 will need propagating quickly butterfly orchid to cultivate to extracting bennet out, if take out bennet, directly cut with scalpel;
Step 2 after the bennet sterilization that cuts, is cut into the segment with a nodal bud, and peels off the outer luxuriant clothing of nodal bud, presses morphologic principle forward oblique cutting on the suitable culture base;
Step 3 is adjusted condition of culture, directly induces nodal bud to be divided into new bennet;
Step 4 continues to cultivate bennet in blake bottle;
Step 5 with the bennet of newly extracting out, directly is cut into the segment with a nodal bud respectively, on the suitable culture base, repeats above-mentioned steps four, step 5 by morphologic forward oblique cutting;
Step 6, repeating step five is until producing band nodal bud bennet;
Step 7 is cut into segment with a nodal bud with the bennet that obtains, and oblique cutting is regulated condition of culture on appropriate media, nodal bud is induced be divided into vegetative bud;
Step 8 is an explant with the vegetative bud, breeds according to a conventional method or differentiation culture;
Step 9 becomes the Moth orchid plantlet with the vegetative bud differentiation culture on the suitable culture base that obtains.
2, according to claim 1ly induce the method for bennet propagating quickly butterfly orchid, it is characterized in that the butterfly orchid variety of described quick breeding need be taken pictures with digital camera, file and preserve parent's image document by the bennet nodal bud.
3, method of inducing the bennet propagating quickly butterfly orchid by the bennet nodal bud according to claim 1, the process that it is characterized in that the sterilization described in the step 2 is for after being stained with 75% alcohol disinfecting with cotton, cut a circumference wound along the periphery of bennet with sharp keen scalpel, fracture with tweezers then.Bennet sections after will rolling over is again inserted in the vial after the sterilization, pours 1.0% liquor natrii hypochloritis sterilization into, with scalpel strip off bract, pours among 0.75% the liquor natrii hypochloritis sterilization again into 5 minutes, takes out with aseptic water washing clean then.
4, according to claim 1ly induce the method for bennet propagating quickly butterfly orchid, it is characterized in that the condition of culture described in the step 3 is that temperature condition is at 18 ℃-23 ℃ by the bennet nodal bud.
5, according to claim 1ly induce the method for bennet propagating quickly butterfly orchid, it is characterized in that bennet needs to be long to 6cm in the step 4 by the bennet nodal bud.
6, according to claim 1ly induce the method for bennet propagating quickly butterfly orchid, it is characterized in that being cut in the step 5 and look the nodal bud number with the quantity of the segment of a nodal bud and how much decide, can be cut into 5 to 6 segments by the bennet nodal bud.
7, according to claim 1ly induce the method for bennet propagating quickly butterfly orchid by the bennet nodal bud, it is characterized in that the condition of culture in the step 7 is 25 ℃, illumination is 14001x.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200610161523XA CN1994068A (en) | 2006-12-27 | 2006-12-27 | Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200610161523XA CN1994068A (en) | 2006-12-27 | 2006-12-27 | Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel |
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| CN1994068A true CN1994068A (en) | 2007-07-11 |
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| CNA200610161523XA Pending CN1994068A (en) | 2006-12-27 | 2006-12-27 | Method for quickly breeding butterfly orchid by using pedicel nodal bud to induct pedicel |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN103004603A (en) * | 2012-12-27 | 2013-04-03 | 福建农林大学 | Plant regeneration method for butterfly orchid |
| CN103202233A (en) * | 2013-04-28 | 2013-07-17 | 安徽农业大学 | Butterfly orchid anti-browning tissue culture method and anti-browning culture medium |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN106942062A (en) * | 2017-04-26 | 2017-07-14 | 江苏农林职业技术学院 | A kind of iris sterile culture strain butt tissue culture method |
| CN107278901A (en) * | 2017-07-31 | 2017-10-24 | 王生旭 | A kind of sterilization method of iris bennet bud |
| CN109601386A (en) * | 2019-01-24 | 2019-04-12 | 北京农业职业学院 | The sterilization method of bud explant is cut in a kind of state orchid stem tip tissue culture |
| CN113575421A (en) * | 2021-08-27 | 2021-11-02 | 中德润萌生态科技(青岛)有限公司 | Aseptic processing method for tissue culture material of orchidaceae plant |
| CN113973713A (en) * | 2021-10-28 | 2022-01-28 | 北京市昌平职业学校 | Butterfly orchid tissue culture method |
-
2006
- 2006-12-27 CN CNA200610161523XA patent/CN1994068A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613076A (en) * | 2012-03-26 | 2012-08-01 | 吴海红 | Vegetative propagation method for butterfly orchid |
| CN103004603A (en) * | 2012-12-27 | 2013-04-03 | 福建农林大学 | Plant regeneration method for butterfly orchid |
| CN103202233A (en) * | 2013-04-28 | 2013-07-17 | 安徽农业大学 | Butterfly orchid anti-browning tissue culture method and anti-browning culture medium |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105104212A (en) * | 2015-10-15 | 2015-12-02 | 山西梅芝园艺有限公司 | Method for controlling high propagation qualified rate of phalaenopsis by utilizing content of hormone |
| CN106942062A (en) * | 2017-04-26 | 2017-07-14 | 江苏农林职业技术学院 | A kind of iris sterile culture strain butt tissue culture method |
| CN107278901A (en) * | 2017-07-31 | 2017-10-24 | 王生旭 | A kind of sterilization method of iris bennet bud |
| CN109601386A (en) * | 2019-01-24 | 2019-04-12 | 北京农业职业学院 | The sterilization method of bud explant is cut in a kind of state orchid stem tip tissue culture |
| CN113575421A (en) * | 2021-08-27 | 2021-11-02 | 中德润萌生态科技(青岛)有限公司 | Aseptic processing method for tissue culture material of orchidaceae plant |
| CN113973713A (en) * | 2021-10-28 | 2022-01-28 | 北京市昌平职业学校 | Butterfly orchid tissue culture method |
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