CN102144566A - Method for culturing test tube plantlet of Xingren Anoectochilus - Google Patents
Method for culturing test tube plantlet of Xingren Anoectochilus Download PDFInfo
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- CN102144566A CN102144566A CN 201110078382 CN201110078382A CN102144566A CN 102144566 A CN102144566 A CN 102144566A CN 201110078382 CN201110078382 CN 201110078382 CN 201110078382 A CN201110078382 A CN 201110078382A CN 102144566 A CN102144566 A CN 102144566A
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- 241000719837 Anoectochilus Species 0.000 title claims abstract description 25
- 239000008619 Xingren Substances 0.000 title claims abstract description 18
- 238000012258 culturing Methods 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title abstract description 7
- 238000012360 testing method Methods 0.000 title abstract description 7
- 229920001817 Agar Polymers 0.000 claims abstract description 8
- 239000008272 agar Substances 0.000 claims abstract description 8
- 239000012882 rooting medium Substances 0.000 claims abstract description 5
- 239000002609 medium Substances 0.000 claims description 14
- 238000005728 strengthening Methods 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- 230000001939 inductive effect Effects 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 abstract 3
- CQEYYJKEWSMYFG-UHFFFAOYSA-N butyl acrylate Chemical compound CCCCOC(=O)C=C CQEYYJKEWSMYFG-UHFFFAOYSA-N 0.000 abstract 3
- 230000006698 induction Effects 0.000 abstract 2
- 239000012883 rooting culture medium Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241000233855 Orchidaceae Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000195940 Bryophyta Species 0.000 description 2
- 235000003140 Panax quinquefolius Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003415 peat Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 206010001557 Albinism Diseases 0.000 description 1
- 241000719836 Anoectochilus formosanus Species 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 241000732800 Cymbidium Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010018367 Glomerulonephritis chronic Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000000496 cardiotonic agent Substances 0.000 description 1
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for culturing a test tube plantlet of Xingren Anoectochilus, which comprises the steps of: selecting a stem section with an axillary bud as an explant, carrying out collateral bud induction and enrichment culture, grafting the collateral bud into a strong seedling rooting culture medium for strong seedling rooting cultivation, and obtaining the test tube plantlet, wherein the culture medium used for collateral bud induction and enrichment culture is MS+(2-5)mg/l 6-BA (butyl acrylate)+(0.2-0.5)mg/l NAA (naphthyl acetic acid)+8g/l agar+30g/l sugar pH: 5.7-6.0; and the strong seedling rooting medium is (1/2MS-MS)+(0.01-0.05)mg/l 6-BA+(0.05-0.10)mg/l NAA+8g/l agar+(15-25)g/l sugar pH: 5.7-6.0. The Xingren Anoectochilus has the propagation coefficient of more than 10 times, so that the test tube plantlet is thick and strong and has the transplant survival rate of more than 95%.
Description
Technical field
The present invention relates to plant tissue and cultivate technical field, particularly relate to the breeding method of Xingren roxburgh anoectochilus terminal bud test-tube plantlet.
Background technology
The Xingren roxburgh anoectochilus terminal bud (
A.xingrenensisZ.H.Tsi et X.H.Jin) be the orchid family (Orchidaceae), open the lip Cymbidium (
Anoectochilus) herbaceos perennial, open general designation roxburgh anoectochilus terminal buds such as lip orchid, anoectochilus formosanus, Kaohsiung roxburgh anoectochilus terminal bud with floral leaf, among the people do medicinal, but anti-inflammatory, detoxifcation, hemostasis step-down, cardiac stimulant diuresis, oxyhepatitis, chronic hepatitis, acute and chronic nephritis, kidney failure, hypertension, diabetes, gynecological disease, cancer etc. there is very significantly curative effect, therefore at the title that " king of medicine, refreshing medicine, gold grass " arranged among the people.According to reports, amino acid composition, composition, content and activity of fighting against senium content of elements all are higher than American Ginseng and wild ginseng in the roxburgh anoectochilus terminal bud.Roxburgh anoectochilus terminal bud is as a kind of precious medicine, and price is very high always, and bright at home careless procurement price reached 400-600 unit/kg in 2010, and the hay price is more up to 1500-2000 unit/kg.Roxburgh anoectochilus terminal bud plant type in Xingren is small and exquisite in addition, and blade profile is attractive in appearance, has high ornamental value.
Xingren roxburgh anoectochilus terminal bud seed only could be sprouted under the situation of mycosymbiosis, and germination rate is low.At natural world, roxburgh anoectochilus terminal bud is the plant of happiness foods such as many worms, bird, mouse, snake.Because mankind's activity, ecotope is damaged, and orders about down in interests, suffers destructive collection again, now has been in critically endangered state.
Summary of the invention
Technical problem to be solved by this invention is to overcome the existing low and endangered defective of wild Xingren roxburgh anoectochilus terminal bud seed germination rate, the kind breeding method of a kind of Xingren roxburgh anoectochilus terminal bud test-tube plantlet is provided, this method is through the screening to medium, optimize and be fit to the medium that Xingren roxburgh anoectochilus terminal bud test-tube plantlet is cultivated, reproduction coefficient reaches more than 10 times, seedling is sturdy, and transplanting survival rate is more than 95%.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme:
The breeding method of Xingren of the present invention roxburgh anoectochilus terminal bud test-tube plantlet: the stem section of select tape axillalry bud is made explant, carries out inducing and enrichment culture from sprouting, and will be transferred to from sprouting then to carry out the strengthening seedling and rooting cultivation the strengthening seedling and rooting medium, promptly gets test-tube plantlet, wherein:
Induce the medium with enrichment culture to be from sprouting:
MS+(2～5) mg/l 6-BA+(0.2～0.5) mg/l NAA+8g/l agar+30g/l sugar pH:5.7～6.0;
The strengthening seedling and rooting medium is:
(mg/l 6-BA+(0.05～0.10) mg/l NAA+8g/l agar of 1/2MS～MS)+(0.01～0.05)+(15～25) g/l sugar pH:5.7～6.0.
In the breeding method of above-mentioned Xingren roxburgh anoectochilus terminal bud test-tube plantlet, when carrying out the strengthening seedling and rooting cultivation, its condition of culture is illumination 16h/d, intensity of illumination 500～1200lux, and 23～27 ℃ of temperature were carried out 1 time to culturing room and sterilized in per 2 months.
Compared with prior art, the screening of the medium that the present invention is adopted when test-tube plantlet is cultivated, optimized the medium that suitable Xingren roxburgh anoectochilus terminal bud test-tube plantlet is cultivated, reproduction coefficient is reached more than 10 times, and the gained test-tube plantlet is sturdy, and the transplanting survival rate of test-tube plantlet can reach more than 95%, in addition, what the present invention adopted is conventional test-tube plantlet breeding method, and this method is simple, ripe, is fit to very much apply.
Embodiment
Describe technical scheme of the present invention in detail below in conjunction with embodiment, the breeding method of Xingren roxburgh anoectochilus terminal bud test-tube plantlet:
(1) foundation of explant selection and sterile system: the plant of selecting robust growth, get the stem section of the long band axillalry bud of 1cm and make explant, the stem section is cleaned out, put into washing powder water rinsing 3～5 minutes earlier, then with flowing water flushing 30 minutes, in superclean bench, with 75% alcohol moistening 5～8 seconds, take out after putting into 0.1% HgCl solution and sterilizing 9～13 minutes, with aseptic water washing 5～6 times;
(2) induce and enrichment culture from sprouting: the explant after the aseptic process is inoculated in from the inducing culture of sprouting, carry out from the inducing culture of sprouting, general inoculation began to sprout in 7～10 days, when sprouting 0.5cm length from sprouting, it is cut open, be forwarded to and make further enrichment culture on the proliferated culture medium, the albefaction seedling that occurs when removing enrichment culture during switching;
Induce screening with the enrichment culture medium from sprouting:
Test is by with the improved culture medium of MS, 1/2MS, MS (macroelement reduces by half, trace element double), and the consumption of 6-BA is from 1～6mg/L, and the consumption of NAA passes through a large amount of screening tests such as orthogonal experiment from 0.1～1.5mg/L.Draw following comparatively ideal medium combination:
MS+(2～5) mg/l 6-BA+(0.2～0.5) mg/l NAA+8g/l agar+30g/l sugar pH:5.7～6.0;
(3) strengthening seedling and rooting is cultivated: from the process of growth of sprouting, while is president's adventive root also, when growing up to the high seedling of 1-2cm from sprouting, be forwarded to and carry out strong plantlets and rootage in the strong plantlets and rootage medium and cultivate, culturing room carried out 1 sterilization in per 2 months, if illumination is strong excessively, the albinism of test-tube plantlet is serious, and the Xingren roxburgh anoectochilus terminal bud is contaminated easily, so per 2 months carry out disinfection to culturing room, can adopt formaldehyde fumigation, culturing room also can adopt ozonization equipment to carry out sterilization;
The screening of strong plantlets and rootage medium:
The strong plantlets and rootage medium is from MS, 1/2MS, 1/4MS, and the consumption of sugar is from 15～30g, and the consumption of 6-BA is from 0.01～0.1, and the NAA consumption filters out following comparatively ideal medium combination from 0.01～0.5mg/L by test of many times such as orthogonal experiments:
(mg/l 6-BA+(0.05～0.10) mg/l NAA+8g/l agar of 1/2MS～MS)+(0.01～0.05)+(15～25) g/l sugar pH:5.7～6.0.
(4) successive transfer culture: in time carry out successive transfer culture behind the strengthening seedling and rooting, guarantee the nutrient supply of medium,, in time change if polluting appears in medium.
(5) acclimatization and transplants: place the normal temperature lower refining seedling after 1 week on blake bottle, take out seedling, clean medium, with plantation comparative trials such as the peat composed of rotten mosses, perlite, wood chip, leaves, wherein the peat composed of rotten mosses is planted roxburgh anoectochilus terminal bud, and survival rate is the highest, can reach more than 95%.
Condition of culture in the above-mentioned cultural method is illumination 16h/d, intensity of illumination 500～1200lux, 23～27 ℃ of temperature.
Claims (2)
1. the breeding method of an Xingren roxburgh anoectochilus terminal bud test-tube plantlet, it is characterized in that: the stem section of select tape axillalry bud is made explant, carries out inducing and enrichment culture from sprouting, and will be transferred to from sprouting then to carry out the strengthening seedling and rooting cultivation the strengthening seedling and rooting medium, promptly get test-tube plantlet, wherein:
Induce the medium with enrichment culture to be from sprouting:
MS+(2～5) mg/l 6-BA+(0.2～0.5) mg/l NAA+8g/l agar+30g/l sugar pH:5.7～6.0;
The strengthening seedling and rooting medium is:
(mg/l 6-BA+(0.05～0.10) mg/l NAA+8g/l agar of 1/2MS～MS)+(0.01～0.05)+(15～25) g/l sugar pH:5.7～6.0.
2. according to the breeding method of the described Xingren of claim 1 roxburgh anoectochilus terminal bud test-tube plantlet, it is characterized in that: the condition of culture that strengthening seedling and rooting is cultivated is illumination 16h/d, intensity of illumination 500～1200lux, and 23～27 ℃ of temperature were carried out 1 time to culturing room and sterilized in per 2 months.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110078382 CN102144566A (en) | 2011-03-30 | 2011-03-30 | Method for culturing test tube plantlet of Xingren Anoectochilus |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511388A (en) * | 2011-11-25 | 2012-06-27 | 贵州师范大学 | Method for breeding anti-reversible tissue culture seedlings of anoectochilus roxburghii |
| CN103120126A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method for carrying out anoectochilus formosanus tissue culture propagation by using intermittent immersion bioreactor |
| CN103688854A (en) * | 2013-12-06 | 2014-04-02 | 四川省自然资源科学研究院 | Tissue culture rapid propagation method of Emei anoectochilus formosanus |
| CN104904600A (en) * | 2015-06-03 | 2015-09-16 | 吴华球 | Tissue-culturing and rapid-propagating method of anoectochilus formosanus |
| CN105475129A (en) * | 2015-05-29 | 2016-04-13 | 三明市农业科学研究院 | Tissue-culture rapid propagation method for arundina graminifolia |
| CN105660400A (en) * | 2016-01-25 | 2016-06-15 | 宁波易中禾生物技术有限公司 | Strengthening and weight increasing method for anoectochilus roxburghii tissue cultured seedlings |
| CN105766647A (en) * | 2016-04-05 | 2016-07-20 | 福建农林大学 | Tissue culture method for club moss |
| CN106386487A (en) * | 2016-09-08 | 2017-02-15 | 福建虎伯寮生物集团有限公司 | Anoectochilus roxburghii tissue culturing method |
| CN106613995A (en) * | 2017-01-18 | 2017-05-10 | 福建农林大学 | Tissue culture method for culturing anoectochilus roxburghii by bacteriostatic culture medium |
| CN109042341A (en) * | 2018-10-24 | 2018-12-21 | 云南善源生物科技发展有限公司 | A kind of roxburgh anoectochilus terminal bud culture medium and preparation method thereof |
-
2011
- 2011-03-30 CN CN 201110078382 patent/CN102144566A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《植物生理学通讯》 19981231 陈穗云 等 台湾金线莲的离体快速繁殖 443 1-2 第34卷, 第6期 * |
| 《现代园艺》 20081031 陈永快 等 福建金线莲和台湾金线莲的组培快繁技术 9-12 1-2 , 第10期 * |
| 《贵州科学》 20070630 张以忠 等 金线莲研究的现状与展望 81-84 1-2 第25卷, 第2期 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511388A (en) * | 2011-11-25 | 2012-06-27 | 贵州师范大学 | Method for breeding anti-reversible tissue culture seedlings of anoectochilus roxburghii |
| CN103120126A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method for carrying out anoectochilus formosanus tissue culture propagation by using intermittent immersion bioreactor |
| CN103120126B (en) * | 2013-02-04 | 2014-05-07 | 南京工业大学 | Method for carrying out anoectochilus formosanus tissue culture propagation by using intermittent immersion bioreactor |
| CN103688854B (en) * | 2013-12-06 | 2016-03-30 | 四川省自然资源科学研究院 | The tissue culture and rapid propagation method of high eyebrow roxburgh anoectochilus terminal bud |
| CN103688854A (en) * | 2013-12-06 | 2014-04-02 | 四川省自然资源科学研究院 | Tissue culture rapid propagation method of Emei anoectochilus formosanus |
| CN105475129A (en) * | 2015-05-29 | 2016-04-13 | 三明市农业科学研究院 | Tissue-culture rapid propagation method for arundina graminifolia |
| CN104904600A (en) * | 2015-06-03 | 2015-09-16 | 吴华球 | Tissue-culturing and rapid-propagating method of anoectochilus formosanus |
| CN105660400A (en) * | 2016-01-25 | 2016-06-15 | 宁波易中禾生物技术有限公司 | Strengthening and weight increasing method for anoectochilus roxburghii tissue cultured seedlings |
| CN105660400B (en) * | 2016-01-25 | 2018-02-13 | 宁波易中禾生物技术有限公司 | A kind of strong sprout weight increasing method of roxburgh anoectochilus terminal bud tissue-cultured seedling |
| CN105766647A (en) * | 2016-04-05 | 2016-07-20 | 福建农林大学 | Tissue culture method for club moss |
| CN105766647B (en) * | 2016-04-05 | 2018-01-12 | 福建农林大学 | A kind of hectolitre pine method for tissue culture |
| CN106386487A (en) * | 2016-09-08 | 2017-02-15 | 福建虎伯寮生物集团有限公司 | Anoectochilus roxburghii tissue culturing method |
| CN106613995A (en) * | 2017-01-18 | 2017-05-10 | 福建农林大学 | Tissue culture method for culturing anoectochilus roxburghii by bacteriostatic culture medium |
| CN109042341A (en) * | 2018-10-24 | 2018-12-21 | 云南善源生物科技发展有限公司 | A kind of roxburgh anoectochilus terminal bud culture medium and preparation method thereof |
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Application publication date: 20110810 |