CN105993952A - Rapid breeding method of Euryodendron excelsum cultivation seedlings - Google Patents
Rapid breeding method of Euryodendron excelsum cultivation seedlings Download PDFInfo
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- CN105993952A CN105993952A CN201610368524.5A CN201610368524A CN105993952A CN 105993952 A CN105993952 A CN 105993952A CN 201610368524 A CN201610368524 A CN 201610368524A CN 105993952 A CN105993952 A CN 105993952A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention provides a rapid breeding method of Euryodendron excelsum cultivation seedlings. The method comprises the steps of explant selection and disinfection, aseptic seedling culture, clustered bud induction, rooting culture, seedling strengthening culture and seedbed culture. Compared with traditional seedling growing methods of Euryodendron excelsum, the method provided by the invention has the advantages of small amount of needed Euryodendron excelsum seeds, realization of short-time mass propagation, keeping of excellent hereditary features of Euryodendron excelsum, good quality of seedlings, low malformed seedling rate, high survival rate of the seedlings reaching 90%, high survival rate of cultivated seedlings reaching 80%, good growth vigor of transplanted nursery stocks, no runt seedlings, effective solving of the problems of increasing reduction of wild resources and difficult natural population updating of Euryodendron excelsum, and provision of technical support for effective protection of the rare and endangered plant Euryodendron excelsum.
Description
Technical field
The invention belongs to technical field of plant propagation, be specifically related to a kind of Sanguis sus domestica wood cultivation Seedling fast breeding side
Method.
Background technology
Sanguis sus domestica wood (Euryodendron excelsum H.T.Chang) belongs to Theaceae (Theaceae)
Sanguis sus domestica wood belongs to perennial woody plant, for the rare or endangered species of the peculiar monotypic genus of China, has been listed in state
Family second class protection plant.The Systematic position of Sanguis sus domestica wood is special, is research Theaceae phylogeny and form
The important materials developed, meanwhile, its trunk is straight and upright, and heartwood is attractive in appearance, and timber is the hardest, is excellent
Construction timber and furniture woods, have certain economic worth.At present, Sanguis sus domestica wood is only distributed in Guangdong Province
Area, county Ba Jia in spring town, and only one population of remaining, individual amount only more than 100 strain in population, it is raw
Long environment is affected seriously by artificial disturbance, and Natural Population faces the danger of extinction.
Sanguis sus domestica wood is unique modes of reproduction with sexual propagation under natural situation, but its seed germination and seedling
Grow affected by environment seriously, Natural population update difficulty.Population quantity is expanded by artificial breeding seedling
And to carry out species population back to nature be the important channel effectively protected Endangered species, but traditional
Seeding growing seedlings method is big to seed demand amount, the protection of serious restriction Sanguis sus domestica these rare and endangered species of wood and
Amount reproduction, and tradition method for culturing seedlings death of seedling rate is up to more than 80%, growth extremely slowly or stops
" little old man's Seedling " phenomenon of growth is serious.Tissue culture is a plant quick breeding technology, needed for it
Plant raw material is few, is not limited by the season of growth, and cultivation cycle is short, makes a variation low, can keep the excellent of female parent
Good inherited character, can be used for saving endangered plants, and solving it cannot the most prolific difficult problem.But mesh
The front Study on tissue culture about Sanguis sus domestica wood have not been reported.
Summary of the invention
It is an object of the invention to solve at least the above and/or defect, and provide after at least and will say
Bright advantage.
It is a still further object of the present invention to provide a kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, utilize Sanguis sus domestica
Wood seed carries out tissue culture, it is thus achieved that Sanguis sus domestica wood seedling, and obtains continuing cultivation in seedling replanting to seedbed
Obtaining Sanguis sus domestica wood cultivation Seedling, this mating system can be effectively improved the coefficient of breeding of Sanguis sus domestica wood, reservation Sanguis sus domestica wood
Excellent inherited character, seedling percent is up to 90%, after cultivation shoot survival percent is up to 80%, and transplanting,
Nursery stock growing way is good, produces without " little old man's Seedling " phenomenon, solves Sanguis sus domestica wood wild resource and day by day reduce,
Natural population updates effective protection of the problem of difficulty, beneficially these rare or endangered species of Sanguis sus domestica wood.
It is a still further object of the present invention to provide a kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, including following
Step:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 24-36h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood
Seed puts into immersion 30min in natural disinfectant liquid, finally by described Sanguis sus domestica wood seed aseptic water washing 3-5
Secondary and remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 10-12 weight portion from generation to generation, 8-10 weight portion white thousand
Layer, 8-10 weight portion Eucalyptus globulus Labill, 7-9 weight portion Bulbus Allii, 4-6 parts by weight of lemon grass and 3-5 weight portion Flos Caryophylli
Being crushed to 50-100 mesh, add immersion 1h under the hydroecium temperature of 500 weight portions, at 60 DEG C, vacuum is returned
Stream extracts 2-4h, filters, and obtains filtrate, then the Bamboo vinegar solution of addition 15-18 weight portion in described filtrate,
Described natural disinfectant liquid is i.e. obtained after mix homogeneously；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 30-35 DEG C,
Cultivate 15-18 days under one illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 1.5-2.0mg/L
GA3(gibberellins), 1.0-1.5mg/L ZT (zeatin), 1.0-1.5mg/L NAA (α-naphthaleneacetic acid),
8-10g/L Fici hispidae juice, 30g/L sucrose and 6g/L agar, pH value is 5.8-6.0；
When cultivating under the first illumination condition, intensity of illumination is 1800-2000lux, and light application time is 3-4h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into the segment of 0.8-1cm also
It is transferred to respectively in inducing clumping bud culture medium, in 25-28 DEG C, after cultivating 2 days under dark condition, proceeds to
Cultivate 28-30 days under second illumination condition, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes
3.0-3.5mg/L ZT, 2.0-2.5mg/L NAA, 1.0-1.5mg/L 6-KT (kinetins), 4-6g/L is big
Bean peptide, 30g/L sucrose and 8g/L agar, pH value is 5.8-6.0；
When cultivating under the second illumination condition, intensity of illumination 1800-2000lux, light application time is 10-12h/
My god；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 26-28 DEG C, dark
Under the conditions of cultivate after 6 days, proceed to cultivate 18-20 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes that 1.5-1.8mg/L is many
Effect azoles, 1.0-1.5mg/L NAA, 0.5-1.0mg/L IBA (indolebutyric acid), 35g/L sucrose and 8g/L
Agar, pH value is 5.8-6.0；
When cultivating under the 3rd illumination condition, intensity of illumination 2000-2200lux, light application time is 10-12h/
My god；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 28-30 days under the 4th illumination condition, obtain young
Seedling, wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.0-1.5mg/L NAA,
1.0-1.5mg/L ZT, 0.5-1.0mg/L paclobutrazol, 3-5g/L activated carbon, 30g/L sucrose and 10g/L fine jade
Fat, pH value is 5.8-6.0；
When cultivating under the 4th illumination condition, intensity of illumination 2000-2200lux, light application time is 10-12h/
My god, during illumination, cultivation temperature is 22-26 DEG C, and during non-illumination, cultivation temperature is 16-20 DEG C；
F, by the described seedling replanting that obtains in step E to seedbed continuing cultivate 5-6 month, obtain Sanguis sus domestica
Wood cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, when seedbed is cultivated, cultivate temperature
Degree is 34-37 DEG C, humidity is 80-90%, the moon degree of covering is 70-80%.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, described seedbed is by bed body and substrate
Composition, wherein, described bed body, comprising:
Bedstead, it is the hollow cuboid of upper surface open, its a length of 3-9m, a width of 1-1.8m, a height of
15-20cm；The right flank being parallel to length direction of described bedstead and the leading flank being adjacent, trailing flank
And bottom surface removably connects；It is 30-60 ° downward-sloping that the lower section of described right flank is provided with vertical direction
Chute, described chute and described bottom edge are fixing to be connected；
Sprayer unit, it inner upper including being disposed on described bedstead the length with described bedstead
The parallel multiple water inlet pipes in direction and the multiple rotary nozzles being disposed on described water inlet pipe；
Attemperating unit, its be the lower inside being arranged on described bedstead undulate arrangement heat-exchange tube；
Automatic control system, it includes the multiple Temperature Humidity Sensors being arranged on the inside of described bedstead and sets
Put the control panel on the outer surface of described leading flank.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, described substrate, comprising:
First hypothallus, it is by 15-20 weight portion rice husk, 10-12 weight portion sawdust, 5-8 weight portion grass
Wood ash and 5-8 weight portion perlite prepare through high temperature sterilize, and its thickness is 6-8cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 20-25 weight portion yellow soil,
15-20 weight portion molasses liquid, 10-15 weight portion bean dregs, 10-15 weight portion straw, 5-10 weight portion matting
Fertile and 0.3-0.5 part enzymatic microorganism is fermented to be prepared, and its thickness is 8-10cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is rotten for the top layer that thickness is 1-2cm
Grow soil.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, GA in described Aseptic seedling culture base3
Concentration be 1.8mg/L, ZT concentration be 1.2mg/L, NAA concentration be 1.2mg/L, Fici hispidae juice
9g/L, pH value is 5.8.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, described inducing clumping bud culture medium
Middle ZT concentration be 3.2mg/L, NAA concentration be 2.2mg/L, 6-KT concentration be 1.2mg/L, greatly
Bean peptide concentration is 5g/L, and pH value is 5.8.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, multiple-effect in described root media
Azoles concentration be 1.6mg/L, NAA concentration be 1.2mg/L, IBA concentration be 0.8mg/L, pH value is 5.8.
Preferably, described Sanguis sus domestica wood cultivation Seedling fast breeding method, NAA in described strong seedling culture base
Concentration be 1.2mg/L, ZT concentration be 1.2mg/L, paclobutrazol concentration is 0.8mg/L, concentration of activated carbon
For 4g/L, pH value is 5.8.
The present invention at least includes following beneficial effect:
The first, compared with Sanguis sus domestica wood tradition method for culturing seedlings, needed for the method for the present invention, Sanguis sus domestica wood seed is few,
Can amount reproduction keep the Sanguis sus domestica excellent hereditary character of wood, seedling quality better in short-term, deformity Seedling rate is low,
Seedling percent is up to 90%, and after cultivation shoot survival percent is up to 80%, and transplanting, nursery stock growing way is good, nothing
" little old man's Seedling " phenomenon produces, and efficiently solves Sanguis sus domestica wood wild resource and day by day reduces, and Natural population is more
The problem of new difficulty, the effectively protection for Sanguis sus domestica these rare or endangered species of wood provides technical support；
The second, Sanguis sus domestica wood seed Fici hispidae juice is soaked, on the one hand Sanguis sus domestica wood seed can be made fully to inhale
Water, promotes seed recovery, on the other hand the active component in Fici hispidae juice can excite Sanguis sus domestica wood seed
Enzyme and the activity of growth hormone, induce Sanguis sus domestica wood seed quick-speed germination；Utilize natural disinfectant liquid to Sanguis sus domestica wood
Seed carries out disinfection, and can avoid traditional ethanol and the infringement to Sanguis sus domestica wood seed of the mercuric chloride sterilization, reduces
Tissue culture procedures produces the probability of deformity Seedling；
3rd, by by GA3, ZT, NAA and Fici hispidae juice adds MS with suitable ratio and cultivates
Base is prepared Aseptic seedling culture base, GA3Synergism can be produced with Fici hispidae juice, collectively promote Sanguis sus domestica
The sprouting of wood seed, protein, aminoacid and the mineral in Fici hispidae juice can be also the life of aseptic seedling
Long providing necessary nutrient substance, ZT and NAA can promote root system and stem and leaf after Sanguis sus domestica wood seed germination
Growth or stretching, extension, so that the aseptic seedling robust plant obtained；
4th, by ZT, NAA, 6-KT and soybean peptide are added in MS culture medium with suitable proportion
Preparing inducing clumping bud culture medium, ZT, NAA and 6-KT combine, and can promote pig in aseptic seedling segment
The propagation of blood wood cell, divide and break up, the formation that induced bundle is sprouted, improves cultivation effect, soybean peptide
In the composition such as small-molecular peptides, free amino acid, saccharide and inorganic salt further promote Sanguis sus domestica wood cell
Growth, breeding, cultivate 28-30 days i.e. available healthy and strong Multiple Buds；
5th, by paclobutrazol, NAA and IBA are added in 3/4MS culture medium with suitable ratio
Prepare root media, the apical dominance of Multiple Buds can be eliminated, stimulate the cell division of Multiple Buds base portion,
Root induction, rooting rate is up to 98%, and well developed root system；
6th, by NAA, ZT, paclobutrazol and activated carbon are added MS culture medium with suitable ratio
Middle preparation strong seedling culture base, NAA, ZT and paclobutrazol can effectively facilitate plant root and the growth of stem and leaf or
Stretch, the addition of activated carbon, growth regulator NAA, ZT and paclobutrazol can be made in strong seedling culture process
In slowly discharge, make plant equal robust growth within the strong seedling culture phase；
7th, by bed body automatic control system, the temperature and humidity in seedbed is controlled at optimum range, can
The problem that in avoiding tradition booth to cultivate, Temperature and Humidity Control is uneven, improves the survival rate of seedling, it is to avoid " little
Old man's Seedling " generation；
8th, growth characteristic and nutritional need according to Sanguis sus domestica wood seedling prepare special culture matrix, base
The first hypothallus in matter is prepared through sterilization by rice husk, sawdust, plant ash and perlite, its loose ventilation,
Water-absorbing-retaining and without pathogen；Second hypothallus is by yellow soil, molasses liquid, bean dregs, straw, barnyard manure
Fermented with enzymatic microorganism prepared, it can be that growth of seedling provides the abundant nutrition being prone to absorb, and fermentation is gone back
Can make the second hypothallus produces substantial amounts of beneficial bacteria, the diseases and insect pests resistance of seedling can be strengthened；3rd
Hypothallus is top layer fertile soil, can be that growth of seedling provides certain nutrition and slows down nutrient in the second hypothallus
With scattering and disappearing of moisture, it is ensured that seedling growing way thereon is good, without pest and disease damage, gained cultivation Seedling is healthy and strong, by it
After carrying out field transplanting, survival rate is high, and growing way is good.
Part is embodied by other advantages, target and the feature of the present invention by description below, and part also will
By the research of the present invention and practice are understood by the person skilled in the art.
Accompanying drawing explanation
Fig. 1 is the structural representation of bed body of the present invention.
Wherein, 1, bedstead；11, right flank；12, leading flank；13, trailing flank；14, bottom surface；15、
Chute；2, water inlet pipe；3, rotary nozzle；4, heat-exchange tube；5, Temperature Humidity Sensor；6, control
Panel.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, to make art technology
Personnel can implement according to this with reference to description word.
It should be noted that experimental technique described in following embodiment, if no special instructions, it is routine
Method, described reagent and material, if no special instructions, the most commercially obtain；The present invention's
In description, term " laterally ", " longitudinally ", " on ", D score, "front", "rear", "left", "right", " perpendicular
Directly ", orientation or the position relationship of the instruction such as " level ", " top ", " end ", " interior ", " outward " is based on attached
Orientation shown in figure or position relationship, be for only for ease of the description present invention and simplify description, not implying that
Show or imply the device of indication or element must have specific orientation, with specific azimuth configuration and operation,
Therefore it is not considered as limiting the invention.
Embodiment 1:
A kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, comprises the following steps:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 24h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood kind
Son puts in natural disinfectant liquid immersion 30min, finally by described Sanguis sus domestica wood seed aseptic water washing 3 times
And remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 10 weight portions from generation to generation, 8 weight portion Cortex Melaleucae leucadendraes, 8
Weight portion Eucalyptus globulus Labill, 7 weight portion Bulbus Alliis, 4 parts by weight of lemon grass and 3 weight portion Flos Caryophylli powder are broken to 50 mesh,
Adding immersion 1h under the hydroecium temperature of 500 weight portions, at 60 DEG C, vacuum back-flow extracts 2h, filters,
Filtrate, then in described filtrate, add the Bamboo vinegar solution of 15 weight portions, i.e. obtain described natural after mix homogeneously
Disinfectant solution；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 30 DEG C, first
Cultivate 15 days under illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 1.5mg/L GA3,
1.0mg/L ZT, 1.0mg/L NAA, 8g/L Fici hispidae juice, 30g/L sucrose and 6g/L agar, pH
Value is 5.8；
When cultivating under the first illumination condition, intensity of illumination is 1800lux, and light application time is 3h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into the segment of 0.8cm also
It is transferred to respectively in inducing clumping bud culture medium, in 25 DEG C, cultivates after 2 days under dark condition, proceed to the
Cultivate 28 days under two illumination conditions, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes 3.0mg/L
ZT, 2.0mg/L NAA, 1.0mg/L 6-KT, 4g/L soybean peptide, 30g/L sucrose and 8g/L agar,
PH value is 5.8；
When cultivating under the second illumination condition, intensity of illumination 1800lux, light application time is 10h/ days；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 26 DEG C, dark bar
After cultivating 6 days under part, proceed to cultivate 18 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes 1.5mg/L multiple-effect
Azoles, 1.0mg/L NAA, 0.5mg/L IBA, 35g/L sucrose and 8g/L agar, pH value is 5.8；
When cultivating under the 3rd illumination condition, intensity of illumination 2000lux, light application time is 10h/ days；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 28 days under the 4th illumination condition, obtain seedling,
Wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.0mg/L NAA,
1.0mg/L ZT, 0.5mg/L paclobutrazol, 3g/L activated carbon, 30g/L sucrose and 10g/L agar, pH value
It is 5.8；
When cultivating under the 4th illumination condition, intensity of illumination 2000lux, light application time is 10h/ days, during illumination
Cultivation temperature is 22 DEG C, and during non-illumination, cultivation temperature is 16 DEG C；
F, the described seedling replanting that will obtain in step E to seedbed continue to cultivate 5 months, obtains Sanguis sus domestica wooden
Cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, when cultivating in seedbed, cultivation temperature is 34 DEG C,
Humidity is 80%, the moon degree of covering is 70%.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described seedbed is made up of bed body and substrate, wherein,
Described bed body as it is shown in figure 1, comprising:
Bedstead 1, it is the hollow cuboid of upper surface open, its a length of 3m, a width of 1m, a height of 15cm；
The right flank 11 being parallel to length direction of described bedstead 1 and the leading flank 12 being adjacent, trailing flank
13 and bottom surface 14 removably connect；The lower section of described right flank 11 be provided with vertical direction be 60 ° to
The chute 15 of lower inclination, described chute 15 is fixing with edge, described bottom surface 14 to be connected；Sprayer unit, its
Including be disposed on described bedstead inner upper and parallel with the length direction of described bedstead multiple enter
Water pipe 2 and the multiple rotary nozzles 3 being disposed on described water inlet pipe；Attemperating unit, it is for arranging
At the heat-exchange tube 4 that the undulate of the lower inside of described bedstead arranges；Automatic control system, its bag
Include multiple Temperature Humidity Sensors 5 of the inside being arranged on described bedstead and be arranged on the appearance of described leading flank
Control panel 6 on face.
The wet operation principle of the temperature automatically controlled control of described bedstead is, the humiture that Temperature Humidity Sensor will collect
Information passes to control panel, and control panel is contrasted with setting value by alignment programs, works as measured value
Time unequal with setting value, control panel is then worked by control programme-control heat-exchange tube and sprayer unit,
During until measured value is equal with setting value, control panel is then by controlling programme-control heat-exchange tube and spraying
Device quits work.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described substrate, comprising:
First hypothallus, it is by 15 weight portion rice husks, 10 weight portion sawdusts, 5 weight portion plant ash and 5
Weight portion perlite prepares through high temperature sterilize, and its thickness is 6cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 20 weight portion yellow soils, 15
Weight portion molasses liquid, 10 weight portion bean dregs, 10 weight portion straws, 5 weight portion barnyard manure and 0.3 part of ferment
Bacterium is fermented to be prepared, and its thickness is 8cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is top layer humic that thickness is 1cm
Soil.
Embodiment 2:
A kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, comprises the following steps:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 30h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood kind
Son puts in natural disinfectant liquid immersion 30min, finally by described Sanguis sus domestica wood seed aseptic water washing 4 times
And remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 11 weight portions from generation to generation, 9 weight portion Cortex Melaleucae leucadendraes, 9
Weight portion Eucalyptus globulus Labill, 8 weight portion Bulbus Alliis, 5 parts by weight of lemon grass and 4 weight portion Flos Caryophylli powder are broken to 80 mesh,
Adding immersion 1h under the hydroecium temperature of 500 weight portions, at 60 DEG C, vacuum back-flow extracts 3h, filters,
Filtrate, then in described filtrate, add the Bamboo vinegar solution of 16 weight portions, i.e. obtain described natural after mix homogeneously
Disinfectant solution；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 32 DEG C, first
Cultivate 16 days under illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 1.8mg/L GA3,
1.2mg/L ZT, 1.2mg/L NAA, 9g/L Fici hispidae juice, 30g/L sucrose and 6g/L agar, pH
Value is 5.8；
When cultivating under the first illumination condition, intensity of illumination is 1900lux, and light application time is 3.5h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into the segment of 0.9cm also
It is transferred to respectively in inducing clumping bud culture medium, in 26 DEG C, cultivates after 2 days under dark condition, proceed to the
Cultivate 29 days under two illumination conditions, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes 3.2mg/L
ZT, 2.2mg/L NAA, 1.2mg/L 6-KT, 5g/L soybean peptide, 30g/L sucrose and 8g/L agar,
PH value is 5.8；
When cultivating under the second illumination condition, intensity of illumination 1900lux, light application time is 11h/ days；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 27 DEG C, dark bar
After cultivating 6 days under part, proceed to cultivate 19 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes 1.6mg/L multiple-effect
Azoles, 1.2mg/L NAA, 0.8mg/L IBA, 35g/L sucrose and 8g/L agar, pH value is 5.8；
When cultivating under the 3rd illumination condition, intensity of illumination 2100lux, light application time is 11h/ days；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 29 days under the 4th illumination condition, obtain seedling,
Wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.2mg/L NAA,
1.2mg/L ZT, 0.8mg/L paclobutrazol, 4g/L activated carbon, 30g/L sucrose and 10g/L agar, pH value
It is 5.8；
When cultivating under the 4th illumination condition, intensity of illumination 2100lux, light application time is 11h/ days, during illumination
Cultivation temperature is 25 DEG C, and during non-illumination, cultivation temperature is 18 DEG C；
F, the described seedling replanting that will obtain in step E to seedbed continue to cultivate 6 months, obtains Sanguis sus domestica wooden
Cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, when cultivating in seedbed, cultivation temperature is 35 DEG C,
Humidity is 85%, the moon degree of covering is 75%.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described seedbed is made up of bed body and substrate, wherein,
Described bed body as it is shown in figure 1, comprising:
Bedstead 1, it is the hollow cuboid of upper surface open, its a length of 6m, a width of 1.5m, a height of
18cm；The right flank 11 being parallel to length direction of described bedstead 1 and the leading flank 12 being adjacent,
Trailing flank 13 and bottom surface 14 removably connect；The lower section of described right flank 11 be provided with vertical direction in
The downward-sloping chute 15 of 60 °, described chute 15 is fixing with edge, described bottom surface 14 to be connected；Spraying
Device, it inner upper including being disposed on described bedstead is the most parallel with the length direction of described bedstead
Multiple water inlet pipes 2 and multiple rotary nozzles 3 of being disposed on described water inlet pipe；Attemperating unit,
It is the lower inside being arranged on described bedstead undulate arrangement heat-exchange tube 4；Automatically control and be
System, it includes the multiple Temperature Humidity Sensors 5 being arranged on the inside of described bedstead and is arranged on described front side
Control panel 6 on the outer surface in face.
The wet operation principle of the temperature automatically controlled control of described bedstead is, the humiture that Temperature Humidity Sensor will collect
Information passes to control panel, and control panel is contrasted with setting value by alignment programs, works as measured value
Time unequal with setting value, control panel is then worked by control programme-control heat-exchange tube and sprayer unit,
During until measured value is equal with setting value, control panel is then by controlling programme-control heat-exchange tube and spraying
Device quits work.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described substrate, comprising:
First hypothallus, it is by 18 weight portion rice husks, 11 weight portion sawdusts, 6 weight portion plant ash and 7
Weight portion perlite prepares through high temperature sterilize, and its thickness is 7cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 23 weight portion yellow soils, 17
Weight portion molasses liquid, 12 weight portion bean dregs, 12 weight portion straws, 8 weight portion barnyard manure and 0.4 part of ferment
Bacterium is fermented to be prepared, and its thickness is 9cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is top layer humic that thickness is 2cm
Soil.
Embodiment 3:
A kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, comprises the following steps:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 36h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood kind
Son puts in natural disinfectant liquid immersion 30min, finally by described Sanguis sus domestica wood seed aseptic water washing 5 times
And remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 12 weight portions from generation to generation, 10 weight portion Cortex Melaleucae leucadendraes,
10 weight portion Eucalyptus globulus Labill, 9 weight portion Bulbus Alliis, 6 parts by weight of lemon grass and 5 weight portion Flos Caryophylli powder are broken to 100
Mesh, adds immersion 1h under the hydroecium temperature of 500 weight portions, and at 60 DEG C, vacuum back-flow extracts 4h, mistake
Filter, obtains filtrate, then adds the Bamboo vinegar solution of 18 weight portions in described filtrate, i.e. obtains described after mix homogeneously
Natural disinfectant liquid；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 35 DEG C, first
Cultivate 18 days under illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 2.0mg/L GA3,
1.5mg/L ZT, 1.5mg/L NAA, 10g/L Fici hispidae juice, 30g/L sucrose and 6g/L agar, pH
Value is 6.0；
When cultivating under the first illumination condition, intensity of illumination is 2000lux, and light application time is 4h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into 1cm segment and point
It is not transferred in inducing clumping bud culture medium, in 28 DEG C, after cultivating 2 days under dark condition, proceeds to second
Cultivate 30 days under illumination condition, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes 3.5mg/L
ZT, 2.5mg/L NAA, 1.5mg/L 6-KT, 6g/L soybean peptide, 30g/L sucrose and 8g/L agar,
PH value is 6.0；
When cultivating under the second illumination condition, intensity of illumination 2000lux, light application time is 12h/ days；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 28 DEG C, dark bar
After cultivating 6 days under part, proceed to cultivate 20 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes 1.8mg/L multiple-effect
Azoles, 1.5mg/L NAA, 1.0mg/L IBA, 35g/L sucrose and 8g/L agar, pH value is 6.0；
When cultivating under the 3rd illumination condition, intensity of illumination 2200lux, light application time is 12h/ days；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 30 days under the 4th illumination condition, obtain seedling,
Wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.5mg/L NAA,
1.5mg/L ZT, 1.0mg/L paclobutrazol, 5g/L activated carbon, 30g/L sucrose and 10g/L agar, pH value
It is 6.0；
When cultivating under the 4th illumination condition, intensity of illumination 2200lux, light application time is 12h/ days, illumination
Time cultivation temperature be 26 DEG C, during non-illumination, cultivation temperature is 20 DEG C；
F, the described seedling replanting that will obtain in step E to seedbed continue to cultivate 6 months, obtains Sanguis sus domestica wooden
Cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, when cultivating in seedbed, cultivation temperature is 37 DEG C,
Humidity is 90%, the moon degree of covering is 80%.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described seedbed is made up of bed body and substrate, wherein,
Described bed body as it is shown in figure 1, comprising:
Bedstead 1, it is the hollow cuboid of upper surface open, its a length of 9m, a width of 1.8m, a height of
20cm；The right flank 11 being parallel to length direction of described bedstead 1 and the leading flank 12 being adjacent,
Trailing flank 13 and bottom surface 14 removably connect；The lower section of described right flank 11 be provided with vertical direction in
The downward-sloping chute 15 of 60 °, described chute 15 is fixing with edge, described bottom surface 14 to be connected；Spraying
Device, it inner upper including being disposed on described bedstead is the most parallel with the length direction of described bedstead
Multiple water inlet pipes 2 and multiple rotary nozzles 3 of being disposed on described water inlet pipe；Attemperating unit,
It is the lower inside being arranged on described bedstead undulate arrangement heat-exchange tube 4；Automatically control and be
System, it includes the multiple Temperature Humidity Sensors 5 being arranged on the inside of described bedstead and is arranged on described front side
Control panel 6 on the outer surface in face.
The wet operation principle of the temperature automatically controlled control of described bedstead is, the humiture that Temperature Humidity Sensor will collect
Information passes to control panel, and control panel is contrasted with setting value by alignment programs, works as measured value
Time unequal with setting value, control panel is then worked by control programme-control heat-exchange tube and sprayer unit,
During until measured value is equal with setting value, control panel is then by controlling programme-control heat-exchange tube and spraying
Device quits work.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described substrate, comprising:
First hypothallus, it is by 20 weight portion rice husks, 12 weight portion sawdusts, 8 weight portion plant ash and 8
Weight portion perlite prepares through high temperature sterilize, and its thickness is 8cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 25 weight portion yellow soils, 20
Weight portion molasses liquid, 15 weight portion bean dregs, 15 weight portion straws, 10 weight portion barnyard manure and 0.5 part of ferment
Bacterium is fermented to be prepared, and its thickness is 10cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is top layer humic that thickness is 2cm
Soil.
Embodiment 4:
A kind of Sanguis sus domestica wood cultivation Seedling fast breeding method, comprises the following steps:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 24h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood kind
Son puts in natural disinfectant liquid immersion 30min, finally by described Sanguis sus domestica wood seed aseptic water washing 5 times
And remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 12 weight portions from generation to generation, 8 weight portion Cortex Melaleucae leucadendraes, 8
Weight portion Eucalyptus globulus Labill, 7 weight portion Bulbus Alliis, 6 parts by weight of lemon grass and 5 weight portion Flos Caryophylli powder are broken to 100 mesh,
Adding immersion 1h under the hydroecium temperature of 500 weight portions, at 60 DEG C, vacuum back-flow extracts 4h, filters,
Filtrate, then in described filtrate, add the Bamboo vinegar solution of 18 weight portions, i.e. obtain described natural after mix homogeneously
Disinfectant solution；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 35 DEG C, first
Cultivate 15 days under illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 2.0mg/L GA3,
1.5mg/L ZT, 1.0mg/L NAA, 10g/L Fici hispidae juice, 30g/L sucrose and 6g/L agar, pH
Value is 5.8；
When cultivating under the first illumination condition, intensity of illumination is 2000lux, and light application time is 3h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into 1cm segment and point
It is not transferred in inducing clumping bud culture medium, in 25 DEG C, after cultivating 2 days under dark condition, proceeds to second
Cultivate 30 days under illumination condition, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes 3.5mg/L
ZT, 2.5mg/L NAA, 1.5mg/L 6-KT, 6g/L soybean peptide, 30g/L sucrose and 8g/L agar,
PH value is 5.8；
When cultivating under the second illumination condition, intensity of illumination 2000lux, light application time is 10h/ days；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 26 DEG C, dark bar
After cultivating 6 days under part, proceed to cultivate 20 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes 1.8mg/L multiple-effect
Azoles, 1.0mg/L NAA, 0.8mg/L IBA, 35g/L sucrose and 8g/L agar, pH value is 5.8；
When cultivating under the 3rd illumination condition, intensity of illumination 2000lux, light application time is 10h/ days；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 28 days under the 4th illumination condition, obtain seedling,
Wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.5mg/L NAA,
1.5mg/L ZT, 0.5mg/L paclobutrazol, 4g/L activated carbon, 30g/L sucrose and 10g/L agar, pH value
It is 5.8；
When cultivating under the 4th illumination condition, intensity of illumination 2000lux, light application time is 10h/ days, during illumination
Cultivation temperature is 26 DEG C, and during non-illumination, cultivation temperature is 16 DEG C；
F, the described seedling replanting that will obtain in step E to seedbed continue to cultivate 6 months, obtains Sanguis sus domestica wooden
Cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, when cultivating in seedbed, cultivation temperature is 34 DEG C,
Humidity is 90%, the moon degree of covering is 80%.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described seedbed is made up of bed body and substrate, wherein,
Described bed body as it is shown in figure 1, comprising:
Bedstead 1, it is the hollow cuboid of upper surface open, its a length of 6m, a width of 1.5m, a height of
20cm；The right flank 11 being parallel to length direction of described bedstead 1 and the leading flank 12 being adjacent,
Trailing flank 13 and bottom surface 14 removably connect；The lower section of described right flank 11 be provided with vertical direction in
The downward-sloping chute 15 of 60 °, described chute 15 is fixing with edge, described bottom surface 14 to be connected；Spraying
Device, it inner upper including being disposed on described bedstead is the most parallel with the length direction of described bedstead
Multiple water inlet pipes 2 and multiple rotary nozzles 3 of being disposed on described water inlet pipe；Attemperating unit,
It is the lower inside being arranged on described bedstead undulate arrangement heat-exchange tube 4；Automatically control and be
System, it includes the multiple Temperature Humidity Sensors 5 being arranged on the inside of described bedstead and is arranged on described front side
Control panel 6 on the outer surface in face.
The wet operation principle of the temperature automatically controlled control of described bedstead is, the humiture that Temperature Humidity Sensor will collect
Information passes to control panel, and control panel is contrasted with setting value by alignment programs, works as measured value
Time unequal with setting value, control panel is then worked by control programme-control heat-exchange tube and sprayer unit,
During until measured value is equal with setting value, control panel is then by controlling programme-control heat-exchange tube and spraying
Device quits work.
Described Sanguis sus domestica wood cultivation Seedling fast breeding method, described substrate, comprising:
First hypothallus, it is by 20 weight portion rice husks, 10 weight portion sawdusts, 5 weight portion plant ash and 8
Weight portion perlite prepares through high temperature sterilize, and its thickness is 8cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 25 weight portion yellow soils, 20
Weight portion molasses liquid, 10 weight portion bean dregs, 10 weight portion straws, 10 weight portion barnyard manure and 0.5 part of ferment
Bacterium is fermented to be prepared, and its thickness is 10cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is top layer humic that thickness is 2cm
Soil.
Comparative example 1:
The beneficial effect of natural disinfectant liquid of the present invention is further illustrated by comparative example 1.Wherein, comparison
The outer implant sterilization of group uses tradition ethanol and mercuric chloride sterilization, and remaining operating condition of matched group is with implementing
Example 2, experimental result is shown in Table 1.
The different sterilization method impact on Sanguis sus domestica wood seedling of table 1
| Sterilization method | Growth coefficient (again) | Deformity Seedling rate (%) |
| Natural disinfectant liquid disinfectant | 5.0 | 0.2 |
| Ethanol and mercuric chloride sterilization | 4.8 | 9 |
As shown in Table 1, the growth coefficient of Sanguis sus domestica wood seedling is affected not notable by sterilization method, but to deformity
Seedling rate has a significant impact, and uses the Sanguis sus domestica wood seedling deformity Seedling rate of natural disinfectant liquid to be only 0.2%, and uses
The Sanguis sus domestica wood seedling abnormal rate of ethanol and mercuric chloride sterilization is up to 9%, illustrates that natural disinfectant liquid disinfectant can be notable
Reduce the lopsided Seedling rate of Sanguis sus domestica wood seedling.
Comparative example 2:
The beneficial effect in seedbed of the present invention is further illustrated by comparative example 2.Wherein, matched group is in institute
Stating uses husky bed to cultivate when seedling in step F is cultivated, and controls temperature by booth, manually adds water management wet
Degree, remaining operating condition of matched group is with embodiment 2, and experimental result is shown in Table 2.
The different training method impact on Sanguis sus domestica wood seedling of table 2
| Training method | Survival rate (%) | Average plant height (cm) | Average leaf number (sheet) |
| Seedbed | 90 | 15.3 | 13 |
| Husky bed | 32 | 8.2 | 9 |
As shown in Table 2, seedbed is used to cultivate, the survival rate of Sanguis sus domestica wood seedling, average plant height and average leaf
Sheet number is all remarkably higher than husky bed and cultivates, and wherein, survival rate improves 181.3%, and average plant height improves 86.6%,
Average leaf number improves 44.4%, illustrates to use seedbed of the present invention to cultivate the survival rate that can not only improve seedling,
Growth of seedling can also be promoted.
Although embodiment of the present invention are disclosed as above, but it is not restricted to description and embodiment party
Listed utilization in formula.It can be applied to various applicable the field of the invention completely.For being familiar with ability
For the personnel in territory, it is easily achieved other amendment.Therefore without departing substantially from claim and etc. homotype
Enclosing under limited general concept, the present invention is not limited to specific details and shown here as the reality with description
Example.
Claims (8)
1. a Sanguis sus domestica wood cultivation Seedling fast breeding method, it is characterised in that comprise the following steps:
A, take maturation Sanguis sus domestica wood seed, be placed in the aqueous solution of the Fici hispidae juice containing 100g/L
After soaking 24-36h at 35 DEG C, take out, after rinsing well with wire tap water, then by described Sanguis sus domestica wood
Seed puts into immersion 30min in natural disinfectant liquid, finally by described Sanguis sus domestica wood seed aseptic water washing 3-5
Secondary and remove its surface moisture with sterilized filter paper, obtain outer implant, wherein,
The preparation method of described natural disinfectant liquid is: by 10-12 weight portion from generation to generation, 8-10 weight portion white thousand
Layer, 8-10 weight portion Eucalyptus globulus Labill, 7-9 weight portion Bulbus Allii, 4-6 parts by weight of lemon grass and 3-5 weight portion Flos Caryophylli
Being crushed to 50-100 mesh, add immersion 1h under the hydroecium temperature of 500 weight portions, at 60 DEG C, vacuum is returned
Stream extracts 2-4h, filters, and obtains filtrate, then the Bamboo vinegar solution of addition 15-18 weight portion in described filtrate,
Described natural disinfectant liquid is i.e. obtained after mix homogeneously；
Described Fici hispidae juice be the bark of Fici hispidae is cut after, the white juice obtained；
B, the described outer implant obtained in step A is placed in Aseptic seedling culture base, in 30-35 DEG C,
Cultivate 15-18 days under one illumination condition, obtain aseptic seedling, wherein,
Described Aseptic seedling culture base is with MS culture medium as minimal medium, also includes 1.5-2.0mg/L
GA3, 1.0-1.5mg/L ZT, 1.0-1.5mg/L NAA, 8-10g/L Fici hispidae juice, 30g/L sucrose
With 6g/L agar, pH value is 5.8-6.0；
When cultivating under the first illumination condition, intensity of illumination is 1800-2000lux, and light application time is 3-4h/ days；
C, the described aseptic seedling sterile scalpel obtained in step B is cut into the segment of 0.8-1cm also
It is transferred to respectively in inducing clumping bud culture medium, in 25-28 DEG C, after cultivating 2 days under dark condition, proceeds to
Cultivate 28-30 days under second illumination condition, obtain Multiple Buds, wherein,
Described inducing clumping bud culture medium is with MS culture medium as minimal medium, also includes
3.0-3.5mg/L ZT, 2.0-2.5mg/L NAA, 1.0-1.5mg/L 6-KT, 4-6g/L soybean peptide, 30g/L
Sucrose and 8g/L agar, pH value is 5.8-6.0；
When cultivating under the second illumination condition, intensity of illumination 1800-2000lux, light application time is 10-12h/
My god；
D, it is transferred to take root in foster base by the described Multiple Buds obtained in step C, in 26-28 DEG C, dark
Under the conditions of cultivate after 6 days, proceed to cultivate 18-20 days under the 3rd illumination condition, obtain whole plant, wherein,
Described root media is with 3/4MS culture medium as minimal medium, also includes that 1.5-1.8mg/L is many
Effect azoles, 1.0-1.5mg/L NAA, 0.5-1.0mg/L IBA, 35g/L sucrose and 8g/L agar, pH value
For 5.8-6.0；
When cultivating under the 3rd illumination condition, intensity of illumination 2000-2200lux, light application time is 10-12h/
My god；
E, in described root media, add the thick sterilized water of 0.5cm, after uncovered cultivation 7 days, by institute
State whole plant to take out, proceed in strong seedling culture base, cultivate 28-30 days under the 4th illumination condition, obtain young
Seedling, wherein,
Described strong seedling culture base is with MS culture medium as minimal medium, also includes 1.0-1.5mg/L NAA,
1.0-1.5mg/L ZT, 0.5-1.0mg/L paclobutrazol, 3-5g/L activated carbon, 30g/L sucrose and 10g/L fine jade
Fat, pH value is 5.8-6.0；
When cultivating under the 4th illumination condition, intensity of illumination 2000-2200lux, light application time is 10-12h/
My god, during illumination, cultivation temperature is 22-26 DEG C, and during non-illumination, cultivation temperature is 16-20 DEG C；
F, by the described seedling replanting that obtains in step E to seedbed continuing cultivate 5-6 month, obtain Sanguis sus domestica
Wood cultivation Seedling, finally carries out field transplanting by described Sanguis sus domestica wood cultivation Seedling.
2. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 1, it is characterised in that seedbed
Middle when cultivating, cultivation temperature is 34-37 DEG C, humidity is 80-90%, the moon degree of covering is 70-80%.
3. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 2, it is characterised in that described
Seedbed is made up of bed body and substrate, wherein, and described bed body, comprising:
Bedstead, it is the hollow cuboid of upper surface open, its a length of 3-9m, a width of 1-1.8m, a height of
15-20cm；The right flank being parallel to length direction of described bedstead and the leading flank being adjacent, trailing flank
And bottom surface removably connects；It is 30-60 ° downward-sloping that the lower section of described right flank is provided with vertical direction
Chute, described chute and described bottom edge are fixing to be connected；
Sprayer unit, it inner upper including being disposed on described bedstead the length with described bedstead
The parallel multiple water inlet pipes in direction and the multiple rotary nozzles being disposed on described water inlet pipe；
Attemperating unit, its be the lower inside being arranged on described bedstead undulate arrangement heat-exchange tube；
Automatic control system, it includes the multiple Temperature Humidity Sensors being arranged on the inside of described bedstead and sets
Put the control panel on the outer surface of described leading flank.
4. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 3, it is characterised in that described
Substrate, comprising:
First hypothallus, it is by 15-20 weight portion rice husk, 10-12 weight portion sawdust, 5-8 weight portion grass
Wood ash and 5-8 weight portion perlite prepare through high temperature sterilize, and its thickness is 6-8cm；
Second hypothallus, it is positioned at above described first hypothallus, its by 20-25 weight portion yellow soil,
15-20 weight portion molasses liquid, 10-15 weight portion bean dregs, 10-15 weight portion straw, 5-10 weight portion matting
Fertile and 0.3-0.5 part enzymatic microorganism is fermented to be prepared, and its thickness is 8-10cm；
3rd hypothallus, it is positioned at above described second hypothallus, and it is rotten for the top layer that thickness is 1-2cm
Grow soil.
5. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 1, it is characterised in that described
GA in Aseptic seedling culture base3Concentration be 1.8mg/L, ZT concentration be that 1.2mg/L, NAA concentration is
1.2mg/L, Fici hispidae juice 9g/L, pH value is 5.8.
6. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 1, it is characterised in that described
In inducing clumping bud culture medium ZT concentration be 3.2mg/L, NAA concentration be that 2.2mg/L, 6-KT are dense
Degree is 1.2mg/L, and soybean peptide concentration is 5g/L, and pH value is 5.8.
7. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 1, it is characterised in that described
In root media paclobutrazol concentration be 1.6mg/L, NAA concentration be that 1.2mg/L, IBA concentration is
0.8mg/L, pH value is 5.8.
8. Sanguis sus domestica wood cultivation Seedling fast breeding method as claimed in claim 1, it is characterised in that described
In strong seedling culture base NAA concentration be 1.2mg/L, ZT concentration be 1.2mg/L, paclobutrazol concentration is
0.8mg/L, concentration of activated carbon is 4g/L, and pH value is 5.8.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110809934A (en) * | 2019-08-26 | 2020-02-21 | 中国科学院华南植物园 | Method for germinating and raising seedlings of haematemesis suis seeds |
| CN112690215A (en) * | 2021-01-14 | 2021-04-23 | 中国科学院华南植物园 | Tissue culture method for Pixumu |
| CN112690214A (en) * | 2021-01-14 | 2021-04-23 | 中国科学院华南植物园 | Breeding method for dracaena cochinchinensis seeds |
| CN114402938A (en) * | 2021-11-26 | 2022-04-29 | 颜培学 | Large-scale breeding and forestation method for Chinese chestnuts |
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| CN110809934A (en) * | 2019-08-26 | 2020-02-21 | 中国科学院华南植物园 | Method for germinating and raising seedlings of haematemesis suis seeds |
| CN112690215A (en) * | 2021-01-14 | 2021-04-23 | 中国科学院华南植物园 | Tissue culture method for Pixumu |
| CN112690214A (en) * | 2021-01-14 | 2021-04-23 | 中国科学院华南植物园 | Breeding method for dracaena cochinchinensis seeds |
| CN112690214B (en) * | 2021-01-14 | 2022-07-08 | 中国科学院华南植物园 | Breeding method of haemateia suis seeds |
| CN114402938A (en) * | 2021-11-26 | 2022-04-29 | 颜培学 | Large-scale breeding and forestation method for Chinese chestnuts |
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| CN105993952B (en) | 2018-07-03 |
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