CN103766135A - New high-yield stable-yield gastrodia elata cultivation technology - Google Patents
New high-yield stable-yield gastrodia elata cultivation technology Download PDFInfo
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- CN103766135A CN103766135A CN201210414838.6A CN201210414838A CN103766135A CN 103766135 A CN103766135 A CN 103766135A CN 201210414838 A CN201210414838 A CN 201210414838A CN 103766135 A CN103766135 A CN 103766135A
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Abstract
The invention discloses a new high-yield stable-yield gastrodia elata cultivation technology. The technology comprises the first step of cultivating first-level mother strains of halimasch, the second step of preparing a second-level primary strain culture medium of the halimasch, the third step of conducting sterilization under atmospheric pressure and inoculated cultivation are conducted on the culture medium, the fourth step of cultivating third-level cultivation strains of the halimasch, the fifth step of cultivating gastrodia elata with the cooperation of the halimasch and new bars, and the sixth step of cultivating gastrodia elata sexual strains with the cooperation of the halimasch strains and germination mushrooms and the new bars. The new high-yield stable-yield gastrodia elata cultivation technology has the advantages that the passage number of the halimasch is reduced and controlled to be two to three, and strong vitality of the halimasch is kept; the strains are cultivated under the indoor condition without infectious microbes, and the stains are not affected by the infectious microbes before the cooperated cultivation between the strains and the gastrodia elata is conducted; rhizomorph breeding is omitted, and therefore a large amount of labor is saved; the cultivation strains of the halimasch are cultivated according to the production requirement and are not affected by external natural environment factors; cultivation can be achieved indoors and outdoors, the yield is high and stable, and two batches of gastrodia elata can be harvested through one-time seeding.
Description
One, technical field
The present invention relates to a kind of culture technique of traditional Chinese medicine, the new technology of especially a kind of high yield, stable yields cultivation rhizoma Gastrodiae.
Two, technical background
Rhizoma Gastrodiae is the conventional rare Chinese medicine of doctor at all times, and the clinical practice history of existing more than 3,000 year so far cures mainly headache, insomnia, dizzy, extremity numbness, epilepsy, tic, child convulsion, high fat of blood, hypertension, tonifying liver, brain tonic; To brain tonic, increasing intelligence, improving eyesight, fixed dizzy, anti-silly, analgesia, to have special efficacy, rhizoma Gastrodiae be the higher medicinal material kinds of present stage economic worth potentiality.
The culture technique that the halimasch companion who promotes the use of now plants rhizoma Gastrodiae is; The female cultivation of planting of one-level halimasch, the cultivation of secondary original seed, the cultivation of three grades of cultivated speciess, the cultivation of bacterium skill, the cultivation of bacterium material, bacterium material add new bar companion and plant rhizoma Gastrodiae, this cultivation method, the one, output is unstable, hohlraum rate is high, output is generally low, its reason is that halimasch passage number is too much, reach more than 5 times, (passage number is best at 2-3 times) mycelium produces and degenerates, the ability of contaminating rhizoma Gastrodiae declines, and Gastrodia that many kinds go down is rotted because of the nutrition supply of failing to obtain in time halimasch; The 2nd, easily bring miscellaneous bacteria and damage by disease and insect into new rhizoma Gastrodiae Jiao Chang, affect Output of Gastrodia elata; The 3rd, efficiency is low, cultivates bacterium material and then bacterium material is taken out to planting rhizoma gastrodiae again, needs a large amount of labours of cost and time.Therefore, invent a kind of high yield, stable yields cultivation rhizoma Gastrodiae new technology and have more using value.
Three, summary of the invention
The object of the invention is: overcome the defect of above-mentioned culture technique, a kind of high yield, stable yields are provided, save time, labour-saving gastrodin cultivation new technology of breeding.
In order to achieve the above object, technical scheme of the present invention is: the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae, the one, halimasch one-level Mother culture, the 2nd, the making of halimasch secondary pedigree seed culture medium, the 3rd, medium is carried out to normal-pressure sterilization and inoculated and cultured, the 4th, three grades of cultivated speciess of halimasch are cultivated, and the 5th, halimasch adds new bar companion and plants rhizoma Gastrodiae, and the 6th, Armillaria mellea and Germination Strain add new bar and cultivate the sexual seed of rhizoma Gastrodiae.
Described halimasch one-level Mother culture, first makes that halimasch is female to be planted, and adopts tissue isolation, or host's partition method obtains bacterial classification, is placed in test tube take potato on main nutrition base, test tube is put into incubator and cultivate.
The making of described halimasch secondary pedigree seed culture medium, by wood sawdust 50%, corn flour 30%, wheat bran 17%, sucrose 2%, land plaster 1%, water damping, mix and make medium, then pack in vial, every bottled carrying capacity is that 4/5ths of bottle carrying capacity is the best, middle with slotting 1 hole of wooden stick, hole depth, to the bottle ends 1/2nd place, is put into sterilization pond after installing.
Described medium is carried out to normal-pressure sterilization and inoculated and cultured, on sterilization pool, resistant to elevated temperatures film is built, surrounding is real with sandbag heap, heat with three steam coppers simultaneously, start to heat with big fire, in 2 hours, make medium temperature reach 100 degree, three steam coppers can add cold water in turn, but must have at least 2 steam coppers producing steam, keep the temperature of medium in pond more than 100 degree, sterilization time is 20～30 hours, medium after sterilizing takes out puts into inoculating hood, being down to 22～27 until temperature inoculates while spending, the secondary original seed connecting kind is placed on cultivation in the room of shading, temperature remains between 20～25 degree.
The cultivation of three grades of cultivated speciess of described halimasch, be first timber to be sawn into a section of 12 centimeter length, be split into the big or small slip of finger, remove without the core of skin and the branch of bifurcated, by wood sawdust, wheat bran, corn flour, sucrose, water furnishing composts or fertilisers of cultivating, with straight angle polypropylene bag splendid attire, upper and lower layer is put composts or fertilisers of cultivating, and spile is put in centre, sealing collapses after installing, carry out normal-pressure sterilization sterilization, then put into inoculating hood and connect bacterium, then take out and put into indoor heat insulating and cultivate.
Can be by directly planting rhizoma Gastrodiae with new bar companion shown in Fig. 1 after growing halimasch silk and shoestring on wood chip, when cultivation, new bar must be cut fish scale mouth, long 30～40 centimetres of material is respectively put 1 wood chip bacterial classification by two place between two green woods, and seeds of Gastrodia elata is near two, respectively put 1, be placed between bacterial classification and new bar, outwardly, seed is put rear covering earth and leaf heat and moisture preserving well to seeds of Gastrodia elata head.
Described Armillaria mellea and sprouting mattress add new bar and cultivate the sexual seed of rhizoma Gastrodiae, its method as shown in Figure 5, two new bars are close to, intermediate space covers in reality, then respectively put 1 wood chip bacterial classification near new bar two, field cultivating, the length at cellar for storing things is depending on landform, after setting bacterial classification, again the Germination Strain leaf that spills the sexual seed of rhizoma Gastrodiae is evenly sprinkled upon to cellar for storing things bed above, and covers earth and leaf heat and moisture preserving.After first rhizoma Gastrodiae is collected, then cover earth and leaf, Second Year can dig out second batch rhizoma Gastrodiae.
The invention has the beneficial effects as follows: the one, reduce the passage number of halimasch, be controlled at 2-3 times, make halimasch keep stronger vigor; The 2nd, bacterial classification is cultivated under without miscellaneous bacteria condition indoor, makes bacterial classification not contaminated by miscellaneous bacteria before planting with rhizoma Gastrodiae companion; The 3rd, save this link of breeding bacterium material, save a large amount of labours; The 4th, cultivate halimasch cultivated species according to need of production, not affected by extraneous factor of natural environment; The 5th, indoor and outdoor all can be cultivated, and output is high and steady, and sowing once can be gathered in the crops two batches of rhizoma Gastrodiaes.
Four, accompanying drawing explanation
Be further described below in conjunction with accompanying drawing and enforcement the present invention:
Fig. 1 is that Armillaria mellea of the present invention adds new bar companion cultivation rhizoma Gastrodiae schematic diagram
Fig. 2 is halimasch one-level Mother culture schematic diagram of the present invention
Fig. 3 is halimasch secondary Primary spawn schematic diagram of the present invention
Fig. 4 is the cultivation schematic diagram of three grades of cultivated speciess of halimasch of the present invention
Fig. 5 is that Armillaria mellea of the present invention and Germination Strain add the sexual seed schematic diagram of new bar cultivation rhizoma Gastrodiae
Five, specific implementation method
The new technology of a kind of high yield of the present invention, stable yields cultivation rhizoma Gastrodiae, as shown in Figures 1 to 5: the one, halimasch one-level Mother culture, the 2nd, the making of halimasch secondary pedigree seed culture medium, the 3rd, medium is carried out to normal-pressure sterilization and inoculated and cultured, the 4th, three grades of cultivated speciess of halimasch are cultivated, the 5th, halimasch adds new bar companion and plants rhizoma Gastrodiae, and the 6th, Armillaria mellea and Germination Strain add bar and cultivate the sexual seed of rhizoma Gastrodiae.
As shown in Figure 2: halimasch one-level Mother culture, first make that halimasch is female to be planted, adopt tissue isolation, or host's partition method obtains bacterial classification, then bacterial classification is placed in test tube take potato on main nutrition base, then test tube is put into incubator and cultivate.After mother's kind is carried out, will carry out halimasch secondary Primary spawn, as shown in Figure 3, first prepare medium, by wood sawdust 50%, corn flour 30%, wheat bran 17%, sucrose 2%, land plaster 1%, water damping, (grab a lot compound with hand and put forth one's strength to pinch and make to expect agglomerating and do not drip, after loosing one's grip i.e. loose being advisable) mixes and makes medium humidity 60% being advisable.Then pack in vial, every bottled carrying capacity is 4/5ths the bests of bottle carrying capacity, middle with slotting 1 hole of wooden stick, hole depth is to the bottle ends 1/2nd place, after installing, put into sterilization pond, carry out normal-pressure sterilization and inoculated and cultured, on sterilization pool, build with resistant to elevated temperatures film, surrounding is real with sandbag heap, heat with three steam coppers simultaneously, start to heat with big fire, in 2 hours, make medium temperature reach 100 degree, three steam coppers can add cold water in turn, but must have at least 2 steam coppers producing steam, keep the temperature of the medium in pond more than 100 degree, sterilization time is 20～30 hours, medium after sterilizing takes out puts into inoculating hood, being down to 22～27 until temperature inoculates while spending, the secondary original seed connecting kind is placed on cultivation in the room of shading, temperature remains between 20～25 degree.Then carry out the cultivation of three grades of cultivated speciess of halimasch, as shown in Figure 4: first timber is sawn into a section of 12 centimeter length, is split into the big or small slip of finger, remove without the core of skin and the branch of bifurcated, by wood sawdust, wheat bran, corn flour, sucrose, water furnishing composts or fertilisers of cultivating, with straight angle polypropylene bag splendid attire, upper and lower layer is put composts or fertilisers of cultivating, and spile is put in centre, sealing collapses after installing, carry out again normal-pressure sterilization sterilization, then put into inoculating hood inoculation, then take out and put into indoor heat insulating cultivation.
Key technology of the present invention, can be by directly planting rhizoma Gastrodiae with new bar companion shown in Fig. 1 after growing halimasch mycelia and shoestring on wood chip, when cultivation, new bar must be cut fish scale mouth, long 30～40 centimetres of material, between two green woods, respectively put 1 wood chip bacterial classification by two place, seeds of Gastrodia elata, near two, is respectively put 1, is placed between bacterial classification and new bar, outwardly, seed is put rear covering earth and leaf heat and moisture preserving well to seeds of Gastrodia elata head.After first rhizoma Gastrodiae is collected, cover earth and leaf, Second Year can take second batch rhizoma Gastrodiae.
Armillaria mellea of the present invention and Germination Strain add new bar and cultivate the sexual seed of rhizoma Gastrodiae, its method as shown in Figure 5, two new bars are close to, intermediate space covers in reality, then respectively put 1 wood chip bacterial classification near new bar two, field cultivating, the length at cellar for storing things is depending on landform, after setting bacterial classification, again the Germination Strain leaf that spills the sexual seed of rhizoma Gastrodiae is evenly sprinkled upon to cellar for storing things bed above, and covers earth and leaf heat and moisture preserving.After first rhizoma Gastrodiae takes out, cover earth and leaf, Second Year can take second batch rhizoma Gastrodiae.
Claims (7)
1. the new technology of a high yield, stable yields cultivation rhizoma Gastrodiae, the one, halimasch one-level Mother culture, the 2nd, the making of halimasch secondary pedigree seed culture medium, the 3rd, medium is carried out to normal-pressure sterilization and inoculated and cultured, the 4th, three grades of cultivated speciess of halimasch are cultivated, the 5th, halimasch adds new bar companion and plants rhizoma Gastrodiae, and the 6th, Armillaria mellea and Germination Strain add new bar and cultivate the sexual seed of rhizoma Gastrodiae.
2. the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae according to claim 1, it is characterized in that: described halimasch one-level Mother culture, first make the female kind of halimasch, adopt tissue isolation, or host's partition method is obtained bacterial classification, be placed in test tube take potato on main nutrition base, test tube put into incubator and cultivate.
3. according to the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae described in right 1, it is characterized in that: the making of described halimasch secondary pedigree seed culture medium, by wood sawdust 50%, corn flour 30%, wheat bran 17%, sucrose 2%, land plaster 1%, water damping, mixes and makes medium, then packs in vial, every bottled carrying capacity is that 4/5ths of bottle carrying capacity is the best, middle with slotting 1 hole of wooden stick, hole depth, to the bottle ends 1/2nd place, is put into sterilization pond after installing.
4. a kind of high yield according to claim 1, the new technology of stable yields cultivation rhizoma Gastrodiae, it is characterized in that: described medium is carried out to normal-pressure sterilization and inoculated and cultured, on sterilization pool, resistant to elevated temperatures film is built, surrounding is real with sandbag heap, heat with three steam coppers simultaneously, start to heat with big fire, in 2 hours, make medium temperature reach 100 degree, three steam coppers can add cold water in turn, but have at least 2 steam coppers producing steam, keep the temperature of medium in pond more than 100 degree, sterilization time is 20～30 hours, medium after sterilizing takes out puts into inoculating hood, being down to 22～27 until temperature inoculates while spending, the secondary original seed connecting kind is placed on cultivation in the room of shading, temperature remains between 20～25 degree.
5. according to the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae described in claim 1, it is characterized in that: the cultivation of three grades of cultivated speciess of described halimasch, first timber to be sawn into a section of 12 centimeter length, be split into the big or small slip of finger, remove without the core of skin and the branch of bifurcated, by wood sawdust, wheat bran, corn flour, sucrose, water furnishing composts or fertilisers of cultivating, with straight angle polypropylene bag splendid attire, upper and lower layer is put composts or fertilisers of cultivating, spile is put in centre, the sealing that collapses after installing, then carry out normal-pressure sterilization sterilization, put into again inoculating hood and connect bacterium, then take out and put into indoor heat insulating cultivation.
6. the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae according to claim 1, it is characterized in that: after growing halimasch silk and shoestring on wood chip, can directly plant rhizoma Gastrodiae with new bar companion, when cultivation, new bar must be cut fish scale mouth, long 30～40 centimetres of material, between two green woods, respectively put 1 wood chip bacterial classification by two place, seeds of Gastrodia elata, near two, is respectively put 1, is placed between bacterial classification and new bar, outwardly, seed is put rear covering earth and leaf heat and moisture preserving well to seeds of Gastrodia elata head.
7. the new technology of a kind of high yield, stable yields cultivation rhizoma Gastrodiae according to claim 1, it is characterized in that: described Armillaria mellea and Germination Strain add new bar and cultivate the sexual seed of rhizoma Gastrodiae, its method is that two new bars are close to, intermediate space covers in reality, then respectively put 1 wood chip bacterial classification near new bar two, field cultivating, the length at cellar for storing things is depending on landform, after setting bacterial classification, again the Germination Strain leaf that spills the sexual seed of rhizoma Gastrodiae is evenly sprinkled upon to cellar for storing things bed above, and covers earth and leaf.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379536A (en) * | 2015-11-20 | 2016-03-09 | 宁书昌 | Gastrodiaelata newly grown tuber expansion period nutrition supply cultivation method |
| CN105646063A (en) * | 2015-04-22 | 2016-06-08 | 储甲松 | Honey fungus mother strain culture medium, culture method of honey fungus strain, method using cotton straws to produce culture medium, and method using culture medium to cultivate rhizoma gastrodiae |
| CN105961008A (en) * | 2016-06-03 | 2016-09-28 | 范金明 | Planting method for Gastrodia elata f. glauca and G. elata. BI. f elata hybrid gastrodia elata |
| CN106045630A (en) * | 2016-06-21 | 2016-10-26 | 德江县绿通天麻发展有限公司 | Rhizoma gastrodiae armillariella mellea culture medium and preparation method thereof |
| CN110122236A (en) * | 2019-05-27 | 2019-08-16 | 王曼 | A kind of gastrodin cultivation method |
| CN111019835A (en) * | 2019-11-01 | 2020-04-17 | 汉中市汉台区汉麓生物科技有限公司 | Method for separating Armillaria mellea from ambary endophytic fungi |
| CN112470841A (en) * | 2020-11-28 | 2021-03-12 | 吉林农业大学 | Method for cultivating gastrodia elata in non-forest land by using straw and mushroom material |
| CN115161204A (en) * | 2022-07-22 | 2022-10-11 | 陕西森盛菌业科技有限公司 | Culture method and culture medium of armillaria mellea for gastrodia elata planting symbiosis |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105646063A (en) * | 2015-04-22 | 2016-06-08 | 储甲松 | Honey fungus mother strain culture medium, culture method of honey fungus strain, method using cotton straws to produce culture medium, and method using culture medium to cultivate rhizoma gastrodiae |
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| CN106045630A (en) * | 2016-06-21 | 2016-10-26 | 德江县绿通天麻发展有限公司 | Rhizoma gastrodiae armillariella mellea culture medium and preparation method thereof |
| CN110122236A (en) * | 2019-05-27 | 2019-08-16 | 王曼 | A kind of gastrodin cultivation method |
| CN110122236B (en) * | 2019-05-27 | 2021-07-13 | 王曼 | Gastrodia elata cultivation method |
| CN111019835A (en) * | 2019-11-01 | 2020-04-17 | 汉中市汉台区汉麓生物科技有限公司 | Method for separating Armillaria mellea from ambary endophytic fungi |
| CN112470841A (en) * | 2020-11-28 | 2021-03-12 | 吉林农业大学 | Method for cultivating gastrodia elata in non-forest land by using straw and mushroom material |
| CN115161204A (en) * | 2022-07-22 | 2022-10-11 | 陕西森盛菌业科技有限公司 | Culture method and culture medium of armillaria mellea for gastrodia elata planting symbiosis |
| CN115161204B (en) * | 2022-07-22 | 2024-04-05 | 陕西森盛菌业科技有限公司 | Culture method and culture medium of armillaria mellea for gastrodia elata planting symbiosis |
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Application publication date: 20140507 |